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Soil microbial biomass and activity strongly depend on land use, vegetation cover, climate, and soil physicochemical properties. In most cases, this dependence was assessed by one-to-one correlations while by employing network analysis, information about network robustness and the balance between stochasticity and determinism controlling connectivity, was revealed. In this study, we further elaborated on the hypothesis of Smith et al. (2021) that cropland soils depended more on climate variables and therefore are more vulnerable to climate change. We used the same dataset with that of Smith et al. (2021) that contains seasonal microbial, climate and soil variables collected from 881 soil points representing the main land uses in Europe: forests, grassland, cropland. We examined complete (both direct and indirect relationships) and incomplete networks (only direct relationships) and recorded higher robustness in the former. Partial Least Square results showed that on average more than 45% of microbial attributes' variability was predicted by climate and habitat drivers denoting medium to strong effect of habitat filtering. Network architecture slightly affected by season or land use type; it followed the core/periphery structure with positive and negative interactions and no hub nodes. Microbial attributes (biomass, activity and their ratio) mostly belong to core block together with Soil Organic Carbon (SOC), while climate and soil variables to periphery block with the exception of cropland networks, denoting the higher dependence between microbial and climate variables in these latter. All complete networks appeared robust except for cropland and forest in summer, a finding that disagrees with our initial hypothesis about cropland. Networks' connectivity was controlled stronger by stochasticity in forest than in croplands. The lack of human interventions in forest soils increase habitat homogeneity enhancing the influence of stochastic agents such as microbial unlimited dispersal and/or stochastic extinction. The increased stochasticity implies the necessity for proactive management actions., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Fires are common in Mediterranean soils and constitute an important driver of their evolution. Although fire effects on vegetation dynamics are widely studied, their influence on the assembly rules of soil prokaryotes in a small-scale environment has attracted limited attention. In the present study, we reanalyzed the data from Aponte et al. (2022) to test whether the direct and/or indirect effects of fire are reflected in the network of relationships among soil prokaryotes in a Chilean sclerophyllous ecosystem. We focused on bacterial (genus and species level) co-occurrence patterns in the rhizospheres and bulk soils in burned and unburned plots. Four soils were considered: bulk-burnt (BB), bulk-unburnt (BU), rhizosphere-burnt (RB), and rhizosphere-unburnt (RU). The largest differences in network parameters were recorded between RU and BB soils, while RB and BU networks exhibited similar values. The network in the BB soil was the most compact and centralized, while the RU network was the least connected, with no central nodes. The robustness of bacterial communities was enhanced in burnt soils, but this was more pronounced in BB soil. The mechanisms mainly responsible for bacterial community structure were stochastic in all soils, whether burnt or unburnt; however, communities in RB were much more stochastic than in RU.

Network analysis was used to show changes in network attributes by analyzing the relations among the main soil microbial groups in a potted tomato soil inoculated with arbuscular mycorrhizal fungus, treated with low doses of Mentha spicata essential oil, or both, and then exposed to tenfold higher oil addition (stress pulse). Pretreatments were chosen since they can induce changes in the composition of the microbial community. Cellular phospholipid fatty acids (PLFAs) and the activity of six soil enzymes, mainly involved in the N-cycle were measured. Networks were constructed based on correlated changes in PLFA abundances. The values of all parameters were significantly different from those of random networks indicating modular architecture. Networks ranked from the lowest to highest modularity: control, non-pretreated and stressed, inoculated and stressed, oil treated and stressed, inoculated and treated with oil and stressed. The high values of network density and 1st/2nd eigenvalue ratio are related to arylamidase activity while N-acetyl-glucosaminidase, acid phosphomoesterase, and asparaginase activities related to high values of the clustering coefficient index. We concluded that modularity may be an efficient indicator of changes in the network of interactions among the members of the soil microbial community and the modular structure of the network may be related to the activity of specific enzymes. Communities that were stressed without a pretreatment were relatively resistant but prone to sudden transition towards instability, while oil or inoculation pretreatments gave networks which could be considered adaptable and susceptible to gradual change.

The arbuscular mycorrhizal fungi (AMF) and the essential oils are both agents of sustainable agriculture, and their independent effects on the community of free-living soil microbes have been explored. In a tomato pot experiment, conducted in a sandy loam mixture, we examined the independent and joint effects of inoculation with the fungus Rhizophagous irregularis and the addition of Mentha spicata essential oil on the structure of the soil microbial community and the activity of soil enzymes involved in the N-cycle, during the pre-symbiosis phase. Plants were grown for 60 days and were inoculated with R. irregularis. Then pots were treated with essential oil (OIL) weekly for a period of a month. Two experimental series were run. The first targeted to examine the effect of inoculation on the microbial community structure by the phospholipid fatty acids analysis (PLFAs), and enzyme activity, and the second to examine the effects of inoculation and essential oil addition on the same variables, under the hypothesis that the joint effect of the two agents would be synergistic, resulting in higher microbial biomass compared to values recorded in singly treated pots. In the AMF pots, N-degrading enzyme activity was dominated by the activity of urease while in the non-inoculated ones by the activities of arylamidase and glutaminase. Higher microbial biomass was found in singly-treated pots (137 and 174% higher in AMF and OIL pots, respectively) compared with pots subjected to both treatments. In these latter pots, higher activity of asparaginase (202 and 162% higher compared to AMF and OIL pots, respectively) and glutaminase (288 and 233% higher compared to AMF and OIL pots, respectively) was found compared to singly-treated ones. Soil microbial biomasses and enzyme activity were negatively associated across all treatments. Moreover, different community composition was detected in pots only inoculated and pots treated only with oil. We concluded that the two treatments produced diverging than synergistic effects on the microbial community composition whereas their joint effect on the activity of asparaginase and glutaminase were synergistic., Competing Interests: Conflict of Interest: All authors declare no conflicts of interest in this paper.

The representation of a complex but stable, self-regulated and, finally, harmonious nature penetrates the whole history of Ecology, thus contradicting the core of the Darwinian evolution. Originated in the pre-Darwinian Natural History, this representation defined theoretically the various schools of early ecology and, in the context of the cybernetic synthesis of the 1950s, it assumed a typical mathematical form on account of α positive correlation between species diversity and community stability. After 1960, these two aforementioned concepts and their positive correlation were proposed as environmental management tools, in the face of the ecological crisis arising at the time. In the early 1970s, and particularly after May's evolutionary arguments, the consensus around this positive correlation collapsed for a while, only to be promptly restored for the purpose of attaching an ecological value on biodiversity. In this paper, we explore the history of the diversity-stability hypothesis and we review the successive terms that have been used to express community stability. We argue that this hypothesis has been motivated by the nodal ideological presuppositions of order and harmony and that the scientific developments in this field largely correspond to external social pressures. We conclude that the conflict about the diversity-stability relationship is in fact an ideological debate, referring mostly to the way we see nature and society rather than to an autonomous scientific question. From this point of view, we may understand why Ecology's concepts and perceptions may decline and return again and again, forming a pluralistic scientific history.

The conflicts around the scientific status of the concept of diversity are considered here as symptoms of hidden, socially originated, ideological representations inherent in the theoretical context of western ecology. Species diversity was coined in the 1940s, as a constant in the statistical models that described the distribution of individuals into different species and, therefore, as the expression of all the parameters that determine ecologically this distribution. The assumption of such a regular distribution is attributed to the influence of organicism and the correlated presuppositions of harmony and homeostasis. Nevertheless, as species diversity was the only unknown parameter in these models, it reversed the direction of the functions and established itself as the main variable under question. After the 1950s, the concept of species diversity was empowered by the strong impact of cybernetics and systems theories; in this context, diversity was considered as a self-regulating mechanism that assures overall stability. Diversity emerges as a natural and one-dimensional measure of community complexity, maturity, and stability. In the perspective of the arising ecological crisis, diversity--because of its property to compare and evaluate--arises as the nodal point of the new scientific/ideological fields of nature conservation and ecosystem management.

In a series of articles, three system ecologists, B. Patten, S. Jørgensen and M. Straskraba attempt to construct a new ecosystem ecology. In this effort, an innovative ecological definition of life that originates from the cognitive domain is prevalent. To be more specific, living entities are defined as those who make models of reality. Through the exploration of this definition, we will try to point out the ontological and the epistemological characteristics of the new ecology. These characteristics facilitate the integration of physicochemical, biological and human systems into a common framework. Indeed, system ecologists attempt to unify the inorganic and the organic world, including human beings, under a universal evolutionary scheme. This scheme is organized around an innovative scientific hypothesi, namely the exergy storage hypothesis.

We examine the emergence of the field of life-history strategies during the 1950s. (We consider a 'field' an area of scientific activity consisting of a theoretical core, a subject of research, a vocabulary and research tools). During the late 1940s and early 1950s, population ecology faced many problems, concerning its conceptual framework, its mathematical models, experimental deficiencies, etc. Research on life-history characteristics remained descriptive, lacking explanations about the causes and significance of phenomena. This was due to the deficiencies of the theoretical framework of population ecology up to the 1950s. The catalyzing factor for the emergence of the new field was the interdisciplinary impacts, and especially the impact of neodarwinism. The elaboration of a new theoretical core, invoking also methodological shifts, was the triggering factor, conditioning the emergence of the new field.