39 results on '"Stamenov, Dragana"'
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2. Biostimulatory activity of root-associated bacillus isolates from nettle (Urtica dioica L.)
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Stamenov Dragana R., Hajnal-Jafari Timea I., Đurić Simonida S., and Hasanagić Dino I.
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inoculation ,medicinal plants ,microorganisms ,plant-growth-promoting rhizobacteria ,Science (General) ,Q1-390 - Abstract
The objective of this research was to isolate bacteria belonging to the genus Bacillus from the nettle rhizosphere, conduct biochemical and plant-growth-promoting (PGP) characterization of isolates, and examine their biostimulatory effects on the seeds of medicinal plants. Microorganisms were isolated using the agar plate method for bacterial isolation. Biochemical characterization involved evaluating the isolates’ ability to produce enzymes such as lipase, amylase, pectinase, protease, cellulose, urease and gelatinase. Plant-growth-promoting (PGP) characterization included evaluation of indole-3-acetic acid (IAA), siderophore and hydrogen cyanide (HCN) production, as well as the ability to mineralize organic phosphorus compounds and solubilize phosphate. The impact of isolates on seed germination and plant growth was studied using summer savory (Satureja hortensis L.) and parsley (Petroselinum crispum L.) seeds. The experiment was conducted under laboratory conditions. The number of germinated seeds, the shoot and root length of seedlings, vigor index and root and shoot length of the plant were measured. According to the morphological description of the colony and cells, five Bacillus bacteria (denoted as Bac4, Bac5, Bac6, Bac7, Bac8) were selected for further examination. All isolates showed good PGP potential. The isolates that stand out are isolates Bac5, Bac7 and Bac8. The best effect on the seed germination was exerted by B5 and Bac8 isolates. Isolates Bac7 and Bac8 had the greatest stimulatory effect on seedling and plant growth. Having great PGP traits, these isolates may represent a biological alternative for the application of harmful pesticides and chemical fertilizers in the production of summer savory and parsley.
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- 2024
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3. Plant growth promoting potential of Bacillus, Azotobacter and Streptomyces bacteria from nettle rhizospheric soil
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Stamenov Dragana, Đurić Simonida, Hajnal Jafari Timea, and Hasanagić Dino
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medicinal herbs ,pgp bacteria ,inoculation ,germination ,Agriculture - Abstract
The significance of employing Plant Growth Promoting (PGP) microorganisms holds immense value in the cultivation of medicinal plants, where the attainment of high-quality plant biomass is indispensable. Therefore, it becomes imperative to isolate and identify a diverse array of microorganisms from the rhizosphere of various plants and assess their efficacy in enhancing the growth of medicinal plants. The primary aim of this research was to isolate and characterize bacteria belonging to the Bacillus, Streptomyces, and Azotobacter genera from the rhizosphere of nettle (Urtica dioica L.). Additionally, the study explored the influence of the bacterial isolates on the germination of oregano (Origanum vulgare L.) and marjoram (Origanum majorana L.) seeds. Isolation, physiological characterization (the growth of isolates at different temperatures, levels of acidity and concentrations of NaCl, and resistance of isolates to Cd and Pb), biochemical characterization (the production of lipase, amylase, pectinase, and cellulase), and PGP characterization of isolates were performed. The impact of isolates on seed germination was monitored under controlled conditions. The count of sprouted seeds was assessed at 7-and 10-day intervals. The results of this study reveal that the isolated rhizospheric bacteria of nettle have multiple physiological, biochemical and PGP properties. All isolates showed good PGP potential, but the isolates Azotobacter A1 and Streptomyces Ac1 stood out. The applied isolates had a positive effect on the seed germination of oregano and marjoram, the best effect being exhibited by Bacillus B2 and Azotobacter A1 on the seed germination of oregano and by Bacillus B1 and Streptomyces Ac1 on that of marjoram.
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- 2024
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4. Hydroponic systems: exploring the balance between co-cultivation of Chlorella vulgaris and Swiss chard (Beta vulgaris L. subsp. cicla)
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Žunić, Vladimira, Jafari, Timea Hajnal, Grabić, Jasna, Đurić, Simonida, and Stamenov, Dragana
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- 2022
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5. Mitigating effect of PGPR on abiotic stress in basil (Ocimum basilicum L.).
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Stamenov, Dragana, Jafari, Timea Hajnal, Djurić, Simonida, Kiprovski, Biljana, and Aćimović, Milica
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PLANT growth-promoting rhizobacteria , *TECHNOLOGICAL innovations , *BACILLUS (Bacteria) , *SUPEROXIDE dismutase , *AZOTOBACTER , *BASIL - Abstract
Having in mind food security and the fact that basil (Ocimum basilicum L.) is used as fresh seasoning, the development of innovative technologies for its cultivation is required. This study aimed to isolate and characterize plant growth-promoting rhizobacteria (PGPR) isolates from basil rhizospheric soil and monitor the effects of their application on basil growth under different water stress conditions. Isolation, determination of isolates biochemical and PGP properties, evaluation of isolates influences on seed germination and parameters of growth of basil plant, growing under well-watered conditions (70% water holding capacity, WHC), under water deficit stress (35% WHC), and flooded conditions (95% WHC), were done. A total of five representative bacterial isolates were selected: Two Azotobacter isolates (A13, A14), one Pseudomonas isolate (P57), and two Bacillus isolates (B79, B82). The results of this experiment revealed that rhizospheric bacteria of O. basilicum L. var. minimum have multiple biochemical and PGP properties. The most intensive reactions on tested abiotic stressors - drought (higher total phenolics, reduced glutathione, and malondialdehyde content) and flooding (higher superoxide dismutase activity) - were from basil plants inoculated with Pseudomonas sp. P57 isolate, yet along with Azotobacter sp. isolate A13. The highest number of germinated seeds was obtained with the A13 isolate (96.0%), while the highest response for vigour index was observed with Azotobacter isolates (7200.0% and 5628.0%). In well-watered conditions, basil mass inoculated with P57 was 34.2% higher than control. In drought-stressed conditions, plant mass inoculated with A13 isolate was 90% higher than control. In flooded-stressed conditions, the plant mass inoculated with Azotobacter and Pseudomonas isolates increased by more than 100%. [ABSTRACT FROM AUTHOR]
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- 2024
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6. Biostimulatory Potential of Microorganisms from Rosemary (Rosmarinus officinalis L.) Rhizospheric Soil
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Stamenov Dragana, Đurić Simonida, and Jafari Timea Hajnal
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medicinal plants ,plant-growth promoting rhizobacteria ,siderophore ,iaa ,Agriculture - Abstract
The objective of the present paper was to isolate microorganisms (Pseudomonas sp., Bacillus sp. and Azotobacter sp.) from the rhizospheric soil of rosemary (Rosmarinus officinalis L.) and investigate their biostimulatory (plant growth-promoting – PGP) and biocontrol potential. The bacteria isolated from the rhizosphere of rosemary included 15 bacteria of the genus Pseudomonas, 20 of the genus Bacillus, and 11 of the genus Azotobacter. Based on the morphological characteristics of colonies and cells, representative isolates of each genus were chosen (marked as Pseudomonas sp. P42, P43, P44; for Bacillus isolates B83, B84, B85, B92, B93; and for Azotobacter isolates A15 and A16) for different physiological and biochemical examination. The study included in vitro screening of the bacterial isolates for their PGP and biocontrol properties. Pseudomonas isolates showed the ability to live at low temperature (10ºC) and high pH (9), and to use different sources of carbon. All Pseudomonas isolates produced lipase, siderophore, hydrogen cyanide, and utilized organic and inorganic phosphorus, while only isolate P42 produced amylase, pectinase and cellulase. Only Bacillus isolates could grow at 45 ºC (all Bacillus isolates), pH 5 (isolates B83), and on a medium containing NaCl 5 and 7% (all isolates). Isolates denoted as B83 and B93 produced lipase, amylase, and pectinase. All isolates had the ability to solubilize phosphate, produce siderophores (except B85) and hydrogen cyanide, while only two isolates (B84 and B85) produced IAA. Azotobacter isolates had the optimal growth at 37ºC and minimal growth on a medium with pH 6 and 9. All Azotobacter isolates used all carbohydrates as a source of carbon and produced lipase, amylase, and hydrogen cyanide. The best result in suppressing the growth of pathogenic fungi Fusarium oxysporum was achieved by using isolate B92. Application of isolate B83 led to the greatest growth suppression of Sclerotinium sclerotiorum.
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- 2021
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7. The Capacity of Soil Microalgae to Improve Germination and Initial Growth of White Radish and Kohlrabi
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Seman Vladimira, Hajnal-Jafari Timea, Đurić Simonida, and Stamenov Dragana
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chlorella sp. ,dictyosphaerium sp. ,white radish ,kohlrabi ,germination ,Agriculture - Abstract
Biofertilizers and biostimulators have become alternative sources of plant nutrients. Biofertilizers derived from microalgae represent a new approach in plant production. The aim of the study was to examine the capacity of the microalgal cell suspension to improve germination and initial growth (root and shoot length) of white Icicle Radish (Raphanus sativus) and Kohlrabi (Brassica oleracea var. gongylodes). By applying soil microalgal biofertilizer, initial growth was improved. Compared to the control, the root length of white radish was increased by 64.24% and 41.32%, using Chlorella sp. and Dictyosphaerium sp. cell suspension, respectively. Kohlrabi root growth was stimulated by application of Chlorella sp. and Dictyosphaerium sp. suspension by 60.97% and 55.02%, respectively. A significant difference in the shoot length was recorded, as well.
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- 2021
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8. New Record of Scenedesmus Vacuolatus From Soil In Vojvodina, Serbia
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Jafari, Timea Hajnal, primary, Žunić, Vladimira, additional, Djurić, Simonida, additional, and Stamenov, Dragana, additional
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- 2023
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9. Characteristics of agricultural organic matter degrading bacterial isolates from different types of soil
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Najvirt Biljana D., Đurić Simonida S., Hajnal-Jafari Timea I., and Stamenov Dragana R.
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bacteria ,characterization ,degradation of aom ,isolation ,soil ,Science (General) ,Q1-390 - Abstract
A large amount of agricultural organic matter (AOM) comes into soil every day, through organic remains, and it is decomposed by bacteria, fungi and actinomycetes (Ugarković et al, 2011). The aim of this research was to select isolates of bacteria with the most organic matter degrading potential, by isolating the bacteria from five different types of soil. Isolation of bacteria was conducted from five types of soil - luvisol, cambisol, chernozem, forest land and meadow. Characterization of bacterial isolates was conducted based on morphological, physiological and biochemical features. Isolates with the most organic matter degrading potential could be used in the near future for conceptualizing microbiological preparation. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. TR 31027]
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- 2019
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10. Microbiological Activity in the Soil of Various Agricultural Crops in Organic Production
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Stamenov Dragana, Đurić Simonida, Jafari Timea Hajnal, Ćirić Vladimir, and Manojlović Maja
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soil ,microorganisms ,rhizosphere ,organic production ,Agriculture - Abstract
The purpose of this study is to investigate the microbial activity and the number of different groups of microorganisms in the soil under organic agricultural systems.
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- 2018
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11. Selektivna izolacija i karakterizacija poljoprivredno korisnih bakterija iz rizosfernog zemljišta Cannabis sativa L., Vojvodina, Srbija
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Stamenov, Dragana, Stamenov, Dragana, Đurić, Simonida, Hajnal Jafari, Timea, Kiprovski, Biljana, Aćimović, Milica, Stamenov, Dragana, Stamenov, Dragana, Đurić, Simonida, Hajnal Jafari, Timea, Kiprovski, Biljana, and Aćimović, Milica
- Abstract
Biljke, uključujući i konoplju (Cannabis sativa L), su domaćini različitih korisnih mikrobnih zajednica. Ovi mikroorganizmi se mogu naći u rizosfernom zemljištu, na biljci ili unutar biljnih tkiva, i svi zajedno čine biljnu mikrobnu zajednicu. Mikroorganizmi označeni kao biljna mikrobna zajednica se sistematski proučavaju dugi niz godina, i velika većina naučne literature se slaže oko njihove centralne uloge u podržavanju rasta, razvoja i opšteg zdravlja biljke. Cilj ovog istraživanja bio je da se iz rizosfernog zemljišta konoplje (Cannabis sativa L) izoluju bakterije roda Pseudomonas, Bacillus i Azotobacter i ispita njihov biostimulatorni (PGP od plant growth-promoting) i biokontrolni potencijal. Ova studija je potvrdila da autohtone rizosferne bakterije konoplje imaju različite sposobnosti tolerancije na abiotičke faktore i više različitih svojstava kojima mogu podstaći rast biljaka. Izolati roda Pseudomonas su pokazali sposobnost da žive na niskim (10ºC) i visokim (37 ºC) temperaturama. Svi izolati su imali minimalan rast na pH 9. Dva izolata označena kao P37 i P39 su tolerisala visoke koncentracije NaCl. Izolati su imali sposobnost da koriste različite izvore ugljenika. Svi Pseudomonas izolati produkovali su lipazu, ureazu, siderofor, cijanovodonik i razlagali jedinjenja organskog i neorganskog fosfora., Plants, including hemp (Cannabis sativa L), host different beneficial microbial communities. These microorganisms can be found in rhizospheric soil, on and inside plants tissues, designated as the plant microbiota or plant-associated microorganisms. The plant-associated microorganisms have been studied systematically for many years, and the vast majority of the scientific literature agrees upon their central role in supporting plant growth, development and overall health. The aim of this study was to isolate bacteria of Pseudomonas, Bacillus, and Azotobacter from the rhizospheric soil of hemp (Cannabis sativa L.) and investigate their biostimulatory (plant growth-promoting – PGP) and biocontrol potential. This study confirmed that indigenous rhizospheric bacteria of hemp have different abiotic stress tolerance abilities and multiple plant growth-promoting (PGP) properties. Pseudomonas isolates showed the ability to live at low (10ºC) and high (37 ºC) temperatures. All isolates had minimal growth on pH 9. Two isolates denoted as P37 and P39 tolerated high concentrations of NaCl. Isolates had the ability to use different sources of carbon. All Pseudomonas isolates produced lipase, urease, siderophore, hydrogen cyanide, and utilized organic and inorganic phosphorus.
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- 2022
12. Nematophagous Activity of Duddingtonia Flagrans MUCL 9827 Against Sheep Gastrointestinal Nematodes
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Simin Stanislav, Đurić Simonida, Kuruca Ljiljana, Hajnal-Jafari Timea, Stamenov Dragana, and Lalošević Vesna
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duddingtonia flagrans ,mucl 9827 ,sheep ,gastro-intestinal nematodes ,nematophagous activity ,Agriculture - Abstract
Gastro-intestinal nematodes (GIN) of sheep are one of major constraints in grazing production systems worldwide. Control is commonly achieved using anthelmintics, but global occurrence of anthelmintic resistance to different drugs and the emergence of multi-resistant GIN species seriously limit the efficiency of their use. Therefore, integrated parasite management is widely recommended, with nematophagous fungi as one of control tools. Duddingtonia flagrans is one of the most used species, with various effect of different isolates. In previously performed coproculture assay, we showed low efficacy of D. flagrans MUCL 9827 against infective larvae (L3) of sheep GIN. The aim of current experiment was to reevaluate its nematophagous potential, using the medium where direct interaction between the fungus and L3 could be observed. Nematophagous activity was tested on 2% water agar with addition of chloramphenicol on three series of plates seeded with 500 and 1000 chlamidospores and agar blocks with 7 days old mycelium. At Days 0 and 5, 500 L3 of sheep GIN were added to test the trapping activity. The cultures, including control plates with only L3, were incubated at 25°C for 10 days, followed by evaluation of their number and reduction percentage. Nematophagous activity of D. flagrans MUCL 9827 against L3 was clearly demonstrated. However, the overall efficacy was poor since trapping was observed only in one out of nine plates containing fungal material. Potential reasons for such poor performance of the isolate of fungal species, otherwise known as successful in trapping animal parasitic nematodes, are discussed.
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- 2016
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13. Microbial Activity in Forest Soil Under Beech, Spruce, Douglas Fir and Fir
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Hajnal-Jafari Timea, Đurić Simonida, Stamenov Dragana, Vasić Verica, and Hackenberger Davorka
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microorganisms ,forest soil ,dehydrogenase ,ß-glucosidase activity ,Agriculture - Abstract
The aim of this research was to investigate the microbial activity in forest soil from different sites under deciduous and coniferous trees in Serbia. One site on Stara planina was under beech trees (Fagus sp.) while another under mixture of spruce (Picea sp.) and Douglas fir (Pseudotsuga sp.). The site on Kopaonik was under mixture of beech (Fagus sp.) and spruce (Picea sp.) trees. The site on Tara was dominantly under fir (Abies sp.), beech (Fagus sp.) and spruce (Picea sp.). The total number of bacteria, the number of actinobacteria, fungi and microorganisms involved in N and C cycles were determined using standard method of agar plates. The activities of dehydrogenase and ß-glucosidase enzymes were measured by spectrophotometric methods. The microbial activity was affected by tree species and sampling time. The highest dehydrogenase activity, total number of bacteria, number of actinobacteria, aminoheterotrophs, amylolytic and cellulolytic microorganisms were determined in soil under beech trees. The highest total number of fungi and number of pectinolytic microorganisms were determined in soil under spruce and Douglas fir trees. The correlation analyses proved the existence of statistically significant interdependency among investigated parameters.
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- 2016
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14. Influence of green algae Chlorella vulgaris on initial growth of different agricultural crops
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Hajnal-Jafari Timea I., Đurić Simonida S., and Stamenov Dragana R.
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green algae ,foliar treatment ,stimulation of growth ,Science (General) ,Q1-390 - Abstract
The aim of this research was to evaluate the effect of green algae Chlorella vulgaris on the initial growth of wheat, maize, bean and lettuce and the microbiological activity of rhizospheric soil. The experiment was conducted in controlled conditions. The inocula were applied as foliar fertilizer by spraying. Plant material was taken 30 days after plant emergance. Chlorella vulgaris affected positively the length (28.5% increase) and fresh mass (17.9% increase) of maize root, stem length of wheat (24.2% increase) and stem mass of lettuce (56.34% increase). Application of Chlorella vulgaris led to the increase of the total number of bacteria and the number of aminoheterotrophs in the maize rhizosphere, total bacterial number in the wheat rhizosphere, and the number of fungi in the rhizosphere of bean. The number of other investigated groups of microorganisms did not change significantly. The activity of dehydrogenase enzyme was not affected by inoculation with green algae.
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- 2016
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15. Bioremediation potential of five strains of Pseudomonas sp.
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Stamenov Dragana R., Đurić Simonida S., and Hajnal-Jafari Timea I.
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bioremediation ,degradation ,petroleum acids ,Pseudomonas sp. ,Science (General) ,Q1-390 - Abstract
Because of their huge biodiversity and metabolic capabilities, the application of microorganisms as bioremediation agents is a way to enhance pollutant degradation. The aim of this research was to investigate the potential of five strains of Pseudomonas sp. as possible bioremediation agents. Strains are from the Collection of the Microbiology Department, Faculty of Agriculture, Novi Sad. Bacterial strains were cultivated in King’s B liquid medium and incubated in shaker at 28°C. Starter culture was obtained after 24h, CFU 108. This 24h old bacterial culture was used for the analysis of influence of five different natural naphthenic acids. Bacterial growth was determined spectrophotometrically through optical density, after 24h and 48h of growth. Our results showed that two bacterial strains (PS V1 and PS2) had better growth after 48h as they used C from the petroleum derivates. The growth of these strains was increased by 72% and 25% with derivates concentration of 10-5 mol/cm3 and 10-6 mol/cm3, respectively. The results of this research showed the potential of certain bacterial strains as bioremediators. [Projekat Ministarstva nauke Republike Srbije, br. TD 31027 i br. III 043002]
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- 2015
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16. Azotobacter, Pseudomonas and Bacillus isolates stimulate the germination and seedling growth of Mellisa officinalis
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Stamenov, Dragana, Stamenov, Dragana, Đurić, Simonida, Hajnal Jafari, Timea, Kiprovski, Biljana, Seman, Vladimira, Stamenov, Dragana, Stamenov, Dragana, Đurić, Simonida, Hajnal Jafari, Timea, Kiprovski, Biljana, and Seman, Vladimira
- Abstract
The objective of this study was the isolation and characterization of Azotobacter, Pseudomonas and Bacillus species from the rhizospheric soil of Mellisa officinalis and to examine the effect of isolates application on the seed germination and seedling growth. Isolation, physiological, biochemical, and plant-growth-promoting activity characterization of isolates were done. Monitoring the effects of isolates application on seed germination and seedling growth were evaluated in controlled conditions. The number of germinated seeds, the shoot and root length of seedlings, vigour index and biochemical stress markers (lipid peroxidation intensity and total phenols) were measured. From the rhizosphere of plant 2 Azotobacter (denoted as A5 and A6), 5 Pseudomonas (denoted as P27, P28, P29, P55 and P56) and 6 Bacillus (denoted as B64, B65, B66, B67, B68 and B69) bacteria were isolated. Azotobacter isolates showed the ability to live in the condition of low (5) and high (9) pH. The isolates varied in terms of the utilization of carbon sources. Both isolates produced pectinase, hydrogen cyanide (HCN) and utilized organic and inorganic phosphorus. Pseudomonas isolates had optimal growth at 10 ºC and 37 ºC, and on medium with pH 5 and 9. All Pseudomonas isolates could produce pectinase, lipase, amylose, IAA, siderophores and HCN. All Bacillus isolates could grow on a medium containing 7% NaCl.
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- 2021
17. The effect of microbial inocula on the growth of black locust, Siberian elm and silver maple seedlings
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Hajnal-Jafari Timea, Jarak Mirjana, Đurić Simonida, Stamenov Dragana, and Orlović Saša
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black locust ,Siberian elm ,silver-leaf maple ,Azotobacterchroococcum ,Streptomyces sp. ,seedling growth ,microorganisms ,Science (General) ,Q1-390 - Abstract
Growth and development of forest plants depend mostly on the soil microbial activity since no mineral or organic fertilizers are applied. Microbial processes can be activated and conditions for plants development improved with the introduction of selected microorganisms in the soil. With the aim of obtaining quality planting material in a shorter period of time, the effects of Azotobacter chroococcum and Streptomyces sp. on the early growth of black locust (Robinia pseudoacacia), Siberian elm (Ulmus pumila) and silver-leaf maple (Acer dasycarpum) were investigated in this study. Microorganisms were applied individually and in a mixture (1:1). Plant height was measured on the 90th, 120th and 180th day after planting. Plant diameter, as well as the number of actinomycetes and azotobacters was measured at the end of the vegetation period (180 days after planting). Applied microorganisms had a positive effect on the seedling height in all three plant species, with the best effect found in the black locust. Effectiveness of applied microorganisms on seedling diameter was the highest in the silver-leaf maple. The largest number of azotobacters was found in the rhizosphere of black locust. Number of microorganisms from both groups was increased in the inoculated variants. [Projekat Ministarstva nauke Republike Srbije, br. III 43002]
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- 2014
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18. Microbiological transformations of phosphorus and sulphur compounds in acid soils
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Stamenov Dragana, Jarak Mirjana, Đurić Simonida, Jafari Hajnal Timea, and Bjelić Dragana
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acid soil ,microorganisms ,phosphorus ,sulphur ,Science (General) ,Q1-390 - Abstract
The dynamics of phosphorus and sulphur in soil is closely related to the dynamics of the biological cycle in which microorganisms play a central role. There is not much microbiological activity in acid soils because aerobes are scarce, rhizosphere is restricted to the shallow surface layer, and the biomass of microorganisms decreases with higher acidity. The aim of the research was to investigate the number of microorganisms, which decompose organic and inorganic phosphorus compounds and organic sulphur compounds in calcocambisol, luvisol, and pseudogley. The following parameters were determined in the soil samples: pH in H2O and in 1MKCl; the content of CaCO3 (%); humus content (%), nitrogen content (%); the content of physiologically active phosphorus and potassium (mg P2O5/100g of soil; mg K2O/100g of soil). The number of microorganisms was determined by the method of agar plates on appropriate nutrient media: the number of microorganisms solubilizing phosphates on a medium by Muramcov; the number of microorganisms that decompose organic phosphorus compounds on a medium with lecithin; and the number of microorganisms that transform organic sulphur compounds on a medium by Baar. All three types of soil are acid non-carbonate soils with a low level of available phosphorus and a more favorable amount of potassium, nitrogen, and humus. The largest number of bacteria, which transform organic phosphorus compounds, was found in calcocambisol. The largest number of phosphate solubilizing bacteria was recorded in pseudogley, whereas the largest number of phosphate solubilizing fungi was recorded in calcocambisol. The largest number of bacteria, which transform organic sulphur compounds, was recorded in pseudogley.
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- 2012
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19. Effect of co-inoculation with different groups of beneficial microorganisms on the microbiological properties of soil and yield of maize (Zea mays L.)
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Hajnal-Jafari Timea, Jarak Mirjana, Đurić Simonida, and Stamenov Dragana
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arbuscular mycorrhizae ,enzymatic activity ,inoculation ,microorganisms ,maize ,PGPB ,PSB ,soils ,yield ,Plant culture ,SB1-1110 ,Biotechnology ,TP248.13-248.65 - Abstract
The effect of co-inoculation on the microbiological properties of rhizospheric soil and yield of maize was investigated in field conditions. The total number of microorganisms (TNM) was largest in variant where Plant Growth Promoting Bacteria (PGPB), Phosphorus Solubilizing Bacteria (PSB) and Arbuscular Mycorrhiza (AM) were applied. TNM was 418.10x107/g soil. The largest number of fungi was achieved in the variant with PGPM (29.65 x104/g soil). The joint use of PGPB, PSB and AM resulted in the largest number of phosphomineralizers (PM) (31.67x104/g soil). Dehydrogenase activity (DHA) increased only in the variant where all the microorganisms were introduced (924μg TPF/10 g soil). The co-inoculation led on average to the increased activity of acid phosphatase (ACP) and alkaline phosphatase (ALP). The highest yield of maize was achieved in the variant with PGPB (15.33 t/ha). Correlation analysis proved a high degree of interdependence between microbiological activity and maize yield.
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- 2012
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20. Autochthonous plant growth-promoting rhizobacteria enhance Thymus vulgaris growth in well-watered and drought-stressed conditions
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Stamenov, Dragana, primary, Djuric, Simonida, additional, Hajnal-Jafari, Timea, additional, and Andjelković, Snežana, additional
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- 2021
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21. Proizvodnja i primena biopreparata
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Timea Hajnal Jafari, Stamenov, Dragana, and Simonida Đurić
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agriculture - Abstract
Udžbenik ‘Proizvodnja i primena biopreparata’’ namenjen je studentima master studija Poljoprivrednog fakulteta u Novom Sadu na studijskim programima Zemljište i ishrana biljaka i Organska poljoprivreda. Iz tog razloga udžbenik je napisan prema postojećem kurikulumu za predmet Prozvodnja i primena biopreparata. Zbog nedostatka literature iz ove oblasti na srpskom jeziku, ovaj udžbenik mogu koristiti i studenti osnovnih studija svih studijsih programa biljne proizvodnje, kao i studenti doktorskih studija Agronomije. Želja nam je bila da na pristupačan, a ipak akademski način studentima približimo tajne života korisnih mikroorganizama iz zemljišta koji se u današnjoj modernoj, organskoj, ekolološki prihvatljivoj poljoprivrednoj proizvodnji u svetu uveliko koriste.
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- 2020
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22. Effect of Chlorella vulgaris on Growth and Photosynthetic Pigment Content in Swiss Chard (Beta vulgaris L. subsp. cicla)
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HAJNAL-JAFARI, TIMEA, primary, SEMAN, VLADIMIRA, additional, STAMENOV, DRAGANA, additional, and ĐURIĆ, SIMONIDA, additional
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- 2020
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23. A COMPARATIVE ANALYSIS OF PLANT GROWTH-PROMOTING TRAITS OF Pseudomonas AND Bacillus STRAINS ISOLATED FROM Lolium perenne RHIZOSPHERIC SOIL IN VOJVODINA (SERBIA) AND THEIR EFFECT ON THE PLANT YIELD
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Stamenov, Dragana, primary, Hajnal-Jafari, Timea I., primary, Najvirt, Biljana, primary, Anđelković, Snežana, primary, Tomić, Jelena, primary, and Đurić, Simonida, primary
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- 2020
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24. The ability of human pathogenic bacteria Escherichia coli O157:H7 to colonize plant tissue
- Author
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Seman, Vladimira, primary, Hajnal-Jafari, Timea, additional, Đurić, Simonida, additional, and Stamenov, Dragana, additional
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- 2020
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25. Response of Microorganisms in Alfalfa Rhizosphere to Microbial Inoculation.
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Andjelković, Snežana, Radović, Jasmina, Babić, Snežana, Vasić, Tanja, Djurić, Simonida, Stamenov, Dragana, and Jafari, Timea Hajnal
- Subjects
ALFALFA ,RHIZOSPHERE ,RHIZOBACTERIA ,DENITRIFYING bacteria ,MICROORGANISMS ,NITROGEN-fixing bacteria ,NITRIFYING bacteria - Abstract
Background: Various microorganisms that can have a positive or negative effect on plant development are present in the rhizospheric soil of alfalfa. The research aimed to investigate the impact of two nitrogen-fixing bacteria and two species of the phytopathogen fungus Colletotrichum on the abundance of aminoheterotrophs, nitrifying and denitrifying bacteria in the rhizosphere of three cultivars of alfalfa. Methods: The experiment was carried in vegetation pots as three factorial, where the first factor was alfalfa cultivar (Affinity+Z, K-28 and Perry), the second was the isolate of phytopathogen fungus Colletotrichum: C. trifolii (isolate Coll 4) and C. destructivum (two isolates: Coll-11 and Coll 657); and the third was the variant of bacterial inoculation (Azotobacter chroococcum and Sinorhizobium meliloti). The number of microorganisms was determined by introducing a diluted soil suspension into proper media and counted per one gram of absolutely dry soil. Result: According to the Fisher test applied inoculation microbial inoculation with nitrogen-fixing bacteria and phytopathogen fungus had different effects on the abundance of examined microorganisms in rizospheric soil of different alfalfa cultivars. [ABSTRACT FROM AUTHOR]
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- 2020
- Full Text
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26. The influence of lead acetate and actinomycetes on germination and growth of vetch plant (Vicia sativa L.)
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Trajkovic, Radmila, primary, Kostic, Milica, additional, Jaksic, Tatjana, additional, Vasic, Predrag, additional, Andjelkovic, Snezana, additional, Babic, Snezana, additional, and Stamenov, Dragana, additional
- Published
- 2018
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27. Influence of Pseudomonas and Bacillus Strains Isolated from Lolium perenne Rhizospheric Soil in Vojvodina (Serbia) on Planth Growth and Soil Microbial Communities
- Author
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STAMENOV, DRAGANA R., primary, DJURIC, SIMONIDA, additional, HAJNAL-JAFARI, TIMEA, additional, and ANDJELKOVIC, SNEŽANA, additional
- Published
- 2017
- Full Text
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28. Microbiological transformations of phosphorus and sulphur compounds in acid soils
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Stamenov, Dragana, Stamenov, Dragana, Jarak, Mirjana, Đurić, Simonida, Jafari, Hajnal Timea, Bjelić, Dragana, Stamenov, Dragana, Stamenov, Dragana, Jarak, Mirjana, Đurić, Simonida, Jafari, Hajnal Timea, and Bjelić, Dragana
- Abstract
The dynamics of phosphorus and sulphur in soil is closely related to the dynamics of the biological cycle in which microorganisms play a central role. There is not much microbiological activity in acid soils because aerobes are scarce, rhizosphere is restricted to the shallow surface layer, and the biomass of microorganisms decreases with higher acidity. The aim of the research was to investigate the number of microorganisms, which decompose organic and inorganic phosphorus compounds and organic sulphur compounds in calcocambisol, luvisol, and pseudogley. The following parameters were determined in the soil samples: pH in H2O and in 1MKCl; the content of CaCO3 (%); humus content (%), nitrogen content (%); the content of physiologically active phosphorus and potassium (mg P2O5/100g of soil; mg K2O/100g of soil). The number of microorganisms was determined by the method of agar plates on appropriate nutrient media: the number of microorganisms solubilizing phosphates on a medium by Muramcov; the number of microorganisms that decompose organic phosphorus compounds on a medium with lecithin; and the number of microorganisms that transform organic sulphur compounds on a medium by Baar. All three types of soil are acid non-carbonate soils with a low level of available phosphorus and a more favorable amount of potassium, nitrogen, and humus. The largest number of bacteria, which transform organic phosphorus compounds, was found in calcocambisol. The largest number of phosphate solubilizing bacteria was recorded in pseudogley, whereas the largest number of phosphate solubilizing fungi was recorded in calcocambisol. The largest number of bacteria, which transform organic sulphur compounds, was recorded in pseudogley., Dinamika fosfora i sumpora u zemljištu tesno je povezana sa dinamikom biološkog ciklusa u kojem mikroorganizmi imaju centralnu ulogu. Mikrobiološka aktivnost u kiselim zemljištima nije velika, jer su potisnuti aerobi, rizosfera je ograničena na plitku površinsku zonu, a sa povećanjem kiselosti smanjuje se i biomasa mikroorganizama. Cilj ovih istraživanja je bio da se ispita zastupljenost mikroorganizama koji razlažu organska i neorganska jedinjenja fosfora i organska jedinjenja sumpora u kalkokambisolu, luvisolu i pseudogleju. U uzorcima zemljišta određeni su sledeći parametri: reakcija zemljišta (pH) u H2O i u 1MKCl; sadržaj CaCO3 (%); sadržaj humusa (%); sadržaj azota (%); sadržaj fiziološki aktivnog fosfora i kalijuma (mg P2O5/100g zemljišta; mg K2O/100g zemljišta). Broj mikroorganizama određivan je metodom agarnih ploča na odgovarajućim selektivnim hranjivim podlogama: broj mikroorganizama koji razlažu fosfate na podlozi po: Muramcov, broj mikroorganizama koji razlažu organska fosforna jedinjenja na podlozi sa lecitinom, a broj mikroorganizama koji transformišu organska jedinjenja sumpora na podlozi po Baar-u. Sva tri tipa zemljišta spadaju u grupu kiselih beskarbonatnih zemljišta, sa niskim sadržajem pristupačnog fosfora i povoljnijim sadržajem kalijuma, azota i humusa. Najveći broj bakterija koje transformišu organska jedinjenja fosfora utvrđen je u kalkokambisolu. Najveći broj bakterija koje razlažu fosfate utvrđen je u pseudogleju, a najveći broj gljiva koje razlažu fosfate u kalkokambisolu. Najveći broj bakterija koje transformišu organska jedinjenja sumpora utvrđen je u pseudogleju.
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- 2012
29. Effect of Chlorella vulgarison Growth and Photosynthetic Pigment Content in Swiss Chard (Beta vulgaris L. subsp. cicla)
- Author
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HAJNAL-JAFARI, TIMEA, SEMAN, VLADIMIRA, STAMENOV, DRAGANA, and ĐURIĆ, SIMONIDA
- Abstract
Microalgae application in agriculture is an alternative measure that could be highly beneficial to plants. The application of microalgae Chlorella vulgarisS45 and its effect on plant growth and pigment content in Swiss chard were investigated. In the treatments, 5% and 10% algal suspensions were applied by spraying on plants and in soil, respectively. C. vulgarisS45 affected the initial growth of Swiss chard and the content of photosynthetic pigments positively. The correlation analysis proved the existence of statistically significant interdependency between chlorophyll a (Chl a) content and leaf number (r = 0.876 at p< 0.05), and chlorophyll b (Chl b) content and fresh leaf weight (r = 0.783 at p< 0.05).
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- 2020
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30. Microbiological analysis of water in the canal Danube-Tisa-Danube
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Stamenov, Dragana, Simonida Đurić, Timea Hajnal-Jafari, and Vojislava Bursić
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Echerichia coli ,water ,canal Danube-Tisa-Danube ,Clostridia sp ,Salmonella sp - Abstract
Contaminated water has a very negative impact on the entire agroecosystem. Surface water is usually polluted through the discharge of pollutants of various origins such as wastewater from industries and households, wastewater from livestock, washing harmful chemicals from the soil into the groundwater, oil spills and more. In this water, there are a variety of harmful and toxic substances, as well as pathogenic microorganisms. The consequences can be disastrous and large-scale. One way of removing unwanted and harmful substances from the wastewater is biological. Biological control meansseparation of harmful substances through the metabolism of microorganisms. The aim of this work was to estimate the microbiological quality of water in the canal Danube-Tisa-Danube (DTD) at two differant locations and the possibility of using this water in agricultural production. Water samples for microbiological analysis were taken from one locality near the village Čelarevo, and two lokalities near Vrbas, outside of direct influence of wastewater flows and its tributaries. Water samples were taken according to the guidelines for taking samples of surface water from rivers and streams ISO 5667-6. Time up period was from January to June 2013. Each sample containing 100ml of water was transferred in the laboratory. Microbiological analysis included the determination of total number of bacteria, the number of Salmonella sp., Echerichia coli and the number of sulphite reducing clostridia (genus Clostridia). Large number of saprophytic bacteria as well as the fluorescent Pseudomonas sp. were found at both lokalities in the investigated time period.The number of Salmonella spp. and E. coli was high indicating the presence of water pollution. Sulphite reducing clostridia were recorded in small numbers, only at the end of the test period, which points to a lack of anaerobic conditions in the canal water. The presented results suggest that the water from the tested localities need to be treated before it is used in agricultural purpose.
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- 2014
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31. Karakterizacija mikoroorganizama promotora rasta i njihovo preživljavanje u rizosferi engleskog ljulja
- Author
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Stamenov, Dragana, Jarak, Mirjana, Jošić, Dragana, and Ćupina, Branko
- Subjects
PGP mikroorganizmi ,PGP microorganisms ,perennial ryegrass ,karakterizacija ,inoculation ,prinos ,characterization ,inokulacija ,yield ,PGP microorganisms, characterization, inoculation, yield, perennial ryegrass ,PGP mikroorganizmi, karakterizacija, inokulacija, prinos, engleski ljulj ,engleski ljulj - Abstract
PGP mikroorganizmi (Plant Growth Promoting) su predmet mnogobrojnih istraživanja, čiji je glavni cilj pronalaženje adekvatnog načina njihove primene u poljoprivredi, hortikulturi, šumarstvu i zaštititi životne sredine. Cilj ovih istraživanja bio je izolacija i karakterizacija mikroorganizama sa PGP svojstvima iz rizosfere engleskog ljulja, ispitivanje sposobnosti njihovog preživljavanja nakon unošenja u zemljište, kao i praćenje uticaja njihove primene na parametre prinosa i mikrobiološku aktivnost u rizosferi biljke. Karakterizacija izolata roda Pseudomonas, Bacillus i Streptomyces obuhvatila je određivanje fizioloških, biohemijskih i PGP osobina. Uticaj introdukcije odabranih izolata i gljive Trichoderma asperellum na brojnost i mikrobiološku aktivnost u rizosferi engleskog ljulja, određivan je standardnim metodama na selektivnim hranljivim podlogama, a dehidrogenazna aktivnost spektrofotometrijskom metodom. U laboratorijskim uslovima ispitivan je efekat primene izolata na klijavost, dužinu korenka i stabaoca klice semena engleskog ljulja. U toku godine, uzimana su tri otkosa i pri tome su određivani dužina nadzemnog dela i korena biljke (cm) i prinos zelene i suve materije nadzemnog dela biljke po otkosu (t/ha). Na osnovu morfoloških, fiziološko-biohemijskih, kao i PGP osobina koje su izolati pokazali, te na osnovu rezultata mnogobrojnih dosadašnjih istraživanja, može se zaključiti da izolati P1 i P9 pripadaju vrsti Pseudomonas putida, izolat P12 Pseudomonas fluorescens, izolati B1, B3 i B6 vrsti Bacillus subtilis, a izolati A1, A2, A3 rodu Streptomyces. Brojnost pojedinih sistematskih i fizioloških grupa mikroorganizama kao i enzimatska aktivnost u rizosferi engleskog ljulja, zavisila je od primenjenih inokulanata. Primena izolata Pseudomonas sp. P12 pozitivno je uticala na povećanje ukupnog broja mikroorganizama, brojnost gljiva, aminoheterotrofa i aktinomiceta. Izolat Bacillus sp. B1 uticao je na povećanje ukupnog broja mikroorganizama, brojnosti gljiva i azotobaktera. Primena izolata Streptomyces sp. A3 dovela je do povećanja broja aktinomiceta i oligonitrofila, dok je primena Trichoderma asperellum uticala pozitivno na povećanje broja aminoheterotrofa i azotobaktera. Primena izolata Pseudomonas sp. P12 i Streptomyces sp. A3 imala je najveći efekat na dehidrogenaznu aktivnost. Inokulacija je imala pozitivan uticaj na klijavost, svežu i suvu masu biljke, visinu i dužinu korena biljaka. Primena gljive Trichoderma asperellum i izolata Streptomyces sp. A3 delovala je pozitivno na klijavost, dužinu korenka i stabaoca klice. U proseku, najbolji efekat na prinos sveže i suve materije, kao i na visinu nadzemnog dela i dužinu korena biljke, imala je primena izolata Pseudomonas sp.P12 i Bacillus sp. B1.U proizvodnji krmnih trava mikroorganizmi još nisu našli značajniju praktičnu primenu iako je veći i kvalitetniji prinos jedan od ciljeva stočarske proizvodnje. Rezultati ovih istraživanja su pokazali da se primenom mikroorganizama mogu postići pozitivni efekti i u proizvodnji engleskog ljulja. Zbog toga je veoma značajno da se vrše dalja ispitivanja uzajamnog odnosa primenjenih mikroorganizama i engleskog ljulja u poljskim uslovima, kako bi se optimizirao način i vreme primene inokulanata, PGP microorganisms (Plant Growth Promoting) have been the subject of many research projects, whose main goal is to find appropriate methods of their use in agriculture, horticulture, forestry and environmental protection. The aim of this study is the isolation and characterization of microorganisms with PGP characteristics from the rhizosphere of perennial ryegrass, testing their ability to survive after entering the soil, and monitoring the impact of their application on yield parameters and microbial activity in the rhizosphere of plants. Characterization of Pseudomonas, Bacillus and Streptomyces included determination of the physiological, biochemical and PGP characteristics. Impact of the introduction of selected isolates and fungi Trichoderma asperellum on quantity and microbial activity in the rhizosphere of ryegrass was determined by using the standard method of selective media; dehydrogenase activity by the spectrophotometric method. The effects of the implementation of isolates on germination, seedling length sprouts seeds of perennial ryegrass were studied under laboratory conditions. During the year, three cuttings were taken. At each of the three cuttings, length of stem and roots of plants (cm) and yields of fresh and dry matter of the plant (t / ha) were measured.Based on morphological, physiological and biochemical characteristics and PGP characteristics observed in the isolates, and based on the results of many previous studies, it can be concluded that (1) P1 and P9 isolates belong to the species Pseudomonas putida, (2)Pseudomonas fluorescens P12 isolate, isolates of B1, B3, B6, belong to the species Bacillus subtilis, and (3) A1, A2 and A3 isolates belong to the ordo Streptomyces. The quantity of systematic and physiological groups of microorganisms and enzymatic activity in the rhizosphere of ryegrass depended on the applied inoculants. Application of Pseudomonas sp. P12 had positive effects on increasing the total number of microorganisms, fungi, aminoheterotrophs and actinomycetes. Isolate Bacillus sp. B1 affected the increase of the total number of micro-organisms in the fungi and Azotobacter. Application of Streptomyces sp. isolates A3 led to an increase in the number of actinomycetes and oligonitrophyls, while the application of Trichoderma asperellum had positive impact on increasing the number aminoheterotrophs and Azotobacter. Application of Pseudomonas sp. P12 and Streptomyces sp. A3 had the greatest effect on dehydrogenase activity. Inoculation had a positive effect on germination, fresh and dry weight of plant, height and rootlength of plants. Introduction of fungus Trichoderma asperellum and Streptomyces sp. A3 isolate acted positively on germination, length of seedling of germs. On average, the best effects on the yield of fresh and dry matter, the height of the stem of the plant and the length of the root were attained by the application of Pseudomonas sp.P12 and Bacillus sp. B1 isolates. Microorganisms have not yet found significant practical use in the production of forage grasses, even though more qualitative yield has been sought in livestock production. The results of this study demonstrate that positive results in the production of perennial ryegrass can be achieved by the use of microorganisms. Therefore, it is very important to carry out further studies of the relationship between applied microorganisms and ryegrass under field conditions in order to optimize the method and time of application of inoculants.
- Published
- 2014
32. Fertilization and crop rotation effects on the number of different groups of microorganisms
- Author
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Stamenov, Dragana, primary, Đurić, Simonida, additional, Hajnal, Jafari Timea, additional, and Šeremešić, Srđan, additional
- Published
- 2016
- Full Text
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33. The occurrence of nitrogen-fixing cyanobacteria, algae and bacteria in different types of soil
- Author
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Jarak, Mirjana, Fojkar, Oliver, Hajnal-Jafari, Timea, Đurić, Simonida, Stamenov, Dragana, and Čolo, Josip
- Subjects
number ,algea ,bacteria ,complex mixtures ,cyanobacteria ,soil - Abstract
The objectives of this study were to determine the frequency of nitrogen-fixing cyanobacteria, algea and total number of bacteria in different types of soil of Vojvodina province. The presence of these groups of microorganisms in the vertical profile of each soil type was also determined. Microorganisms were studied in five soil types: chernozem, fluvisol, arenosol, solonetz and humogley. The highest number of nitrogen- fixing cyanobacteria and bacteria was obtained in solonetz while the highest number of algae was determined in fluvisol. The number of investigated groups of microorganisms in the analyzed soils were found to be depended on soil type and soil profile depth. The numbers of the microorganisms under study typically decreased with soil depth. The obtained results show us that the strains of nitrogen-fixing cyanobacteria isolated from local soil may be used through biotechnological application in the development of microbiological inocula which are effective in temperate climatic conditions.
- Published
- 2012
34. Karakterizacija mikoroorganizama promotora rasta i njihovo preživljavanje u rizosferi engleskog ljulja
- Author
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Jarak, Mirjana, Jošić, Dragana, Ćupina, Branko, Stamenov, Dragana, Jarak, Mirjana, Jošić, Dragana, Ćupina, Branko, and Stamenov, Dragana
- Abstract
PGP mikroorganizmi (Plant Growth Promoting) su predmet mnogobrojnih istraživanja, čiji je glavni cilj pronalaženje adekvatnog načina njihove primene u poljoprivredi, hortikulturi, šumarstvu i zaštititi životne sredine. Cilj ovih istraživanja bio je izolacija i karakterizacija mikroorganizama sa PGP svojstvima iz rizosfere engleskog ljulja, ispitivanje sposobnosti njihovog preživljavanja nakon unošenja u zemljište, kao i praćenje uticaja njihove primene na parametre prinosa i mikrobiološku aktivnost u rizosferi biljke. Karakterizacija izolata roda Pseudomonas, Bacillus i Streptomyces obuhvatila je određivanje fizioloških, biohemijskih i PGP osobina. Uticaj introdukcije odabranih izolata i gljive Trichoderma asperellum na brojnost i mikrobiološku aktivnost u rizosferi engleskog ljulja, određivan je standardnim metodama na selektivnim hranljivim podlogama, a dehidrogenazna aktivnost spektrofotometrijskom metodom. U laboratorijskim uslovima ispitivan je efekat primene izolata na klijavost, dužinu korenka i stabaoca klice semena engleskog ljulja. U toku godine, uzimana su tri otkosa i pri tome su određivani dužina nadzemnog dela i korena biljke (cm) i prinos zelene i suve materije nadzemnog dela biljke po otkosu (t/ha). Na osnovu morfoloških, fiziološko-biohemijskih, kao i PGP osobina koje su izolati pokazali, te na osnovu rezultata mnogobrojnih dosadašnjih istraživanja, može se zaključiti da izolati P1 i P9 pripadaju vrsti Pseudomonas putida, izolat P12 Pseudomonas fluorescens, izolati B1, B3 i B6 vrsti Bacillus subtilis, a izolati A1, A2, A3 rodu Streptomyces. Brojnost pojedinih sistematskih i fizioloških grupa mikroorganizama kao i enzimatska aktivnost u rizosferi engleskog ljulja, zavisila je od primenjenih inokulanata. Primena izolata Pseudomonas sp. P12 pozitivno je uticala na povećanje ukupnog broja mikroorganizama, brojnost gljiva, aminoheterotrofa i aktinomiceta. Izolat Bacillus sp. B1 uticao je na povećanje ukupnog broja mikroorganizama, brojnosti gljiva i azot, PGP microorganisms (Plant Growth Promoting) have been the subject of many research projects, whose main goal is to find appropriate methods of their use in agriculture, horticulture, forestry and environmental protection. The aim of this study is the isolation and characterization of microorganisms with PGP characteristics from the rhizosphere of perennial ryegrass, testing their ability to survive after entering the soil, and monitoring the impact of their application on yield parameters and microbial activity in the rhizosphere of plants. Characterization of Pseudomonas, Bacillus and Streptomyces included determination of the physiological, biochemical and PGP characteristics. Impact of the introduction of selected isolates and fungi Trichoderma asperellum on quantity and microbial activity in the rhizosphere of ryegrass was determined by using the standard method of selective media; dehydrogenase activity by the spectrophotometric method. The effects of the implementation of isolates on germination, seedling length sprouts seeds of perennial ryegrass were studied under laboratory conditions. During the year, three cuttings were taken. At each of the three cuttings, length of stem and roots of plants (cm) and yields of fresh and dry matter of the plant (t / ha) were measured. Based on morphological, physiological and biochemical characteristics and PGP characteristics observed in the isolates, and based on the results of many previous studies, it can be concluded that (1) P1 and P9 isolates belong to the species Pseudomonas putida, (2)Pseudomonas fluorescens P12 isolate, isolates of B1, B3, B6, belong to the species Bacillus subtilis, and (3) A1, A2 and A3 isolates belong to the ordo Streptomyces. The quantity of systematic and physiological groups of microorganisms and enzymatic activity in the rhizosphere of ryegrass depended on the applied inoculants. Application of Pseudomonas sp. P12 had positive effects on increasing the total number of microorganisms, fungi
- Published
- 2014
35. Plant Growth Promotion Rhizobacteria in Onion Production
- Author
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ČOLO, JOSIP, primary, HAJNAL-JAFARI, TIMEA I., additional, ĐURIĆ, SIMONIDA, additional, STAMENOV, DRAGANA, additional, and HAMIDOVIĆ, SAUD, additional
- Published
- 2014
- Full Text
- View/download PDF
36. Effects of plant growth promoting rhizobacteria on maize in greenhouse and field trial
- Author
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Jarak, Mirjana, Jarak, Mirjana, Mrkovački, Nastasija, Bjelić, Dragana, Jošić, Dragana, Hajnal-Jafari, Timea, Stamenov, Dragana, Jarak, Mirjana, Jarak, Mirjana, Mrkovački, Nastasija, Bjelić, Dragana, Jošić, Dragana, Hajnal-Jafari, Timea, and Stamenov, Dragana
- Abstract
The aim of the study was to investigate ability of Pseudomonas sp. Q4b, Bacillus sp. Q5a and Azotobacter chroococcum strain 8 to enrich a population of plant growth promoting rhizobacteria (PGPR) in rhizosphere and thus enhance maize growth (Zea mays L.). Experiments were carried out under greenhouse and field conditions, on a chernozem soil. In vitro screening for PGP properties showed significant difference between indole-3-acetic acid (IAA) and siderophores production and phosphosolubilization between Pseudomonas sp. Q4b and Bacillus sp. Q5a. Pseudomonas was estimated as a better producer of hydrogen cyanide (HCN) and siderophores than Bacillus. Bacterial treatments had a significant effect on the number of investigated microbial groups in rhizosphere, and maize growth and yield. Co-inoculation had an advantage compared to single inoculation, while in case of single strains, better effects were achieved in Pseudomonas and Azotobacter treatments. Inoculation increased the number of azotobacters (103.25%), pseudomonads (82.29%) and aerobic spore-forming bacteria (52.65%) as well as height (17.15%) and dry weight (35.48%) of maize plants. The highest plant height was recorded in treatment with Pseudomonas and Bacillus. The highest dry weight and yield were recorded in the treatment with mixture of these three strains.
- Published
- 2012
37. YIELD RESPONSE OF BEAN TO DIFFERENT AGRICULTURAL PRACTICES AND MICROBIAL ACTIVITY IN ITS RHIZOSPHERE.
- Author
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Đurić, Simonida, Stamenov, Dragana, Hajnal-Jafari, Timea, Petrović, Bojana, and Vasić, Mirjana
- Subjects
- *
KIDNEY bean yields , *RHIZOSPHERE , *BIOFERTILIZERS , *ORGANIC farming , *CROP management - Abstract
The aim of this study was to compare the effect of organic and conventional production on yield parameters of bean (Phaseolus vulgaris) as well as on the number of rhizospheric microorganisms. Studies were conducted at two sites on chernosem soil. Organic production was conducted on the field in the village Pivnice, while conventional production was conducted in the village Čurug, Serbia during 2014. Varieties of Balkan, Zlatko, Belko, Sremac, Slavonac, Maksa, and 20-tica were used. Before sowing, the bean seeds were inoculated by biofertilizers NS-Nitragin for beans and faba beans. NS-Nitragin contains a mixture of selected strains of symbiotic bacteria Rhizobium leguminosarum bv. phaseoli. Laboratory measurements were performed in microbiological laboratories of Faculty of Agriculture, Novi Sad and in the Institute of Field and Vegetable Crops, Novi Sad. The height and mass of the plant, the number and weight of the bean seeds per plant as well as the number of microorganisms in its rhizosphere (the total number, number of azotobacter, actinomycetes and fungi) were determined. In this research, the average values of all examined parameters of plant yields were lower in the organic production compared to conventional, except for variety Slavonac. In this case it was obtained a statistically significant increas of plant mass, number and weight of seeds per plant in organic production compared to conventional. Those results indicate that the variety Slavonac should be grown in organic production. The results showed that inoculation can have a positive effect on the number of microorganisms in the rhizosphere of the bean. The number of the investigated groups of microorganisms, apart from actinomycetes, increased in the organic production, where microbiological fertilizers were applied. In average, statistical analises showed that inoculation did not have a statistically significant impact on the number of the investigated groups of microorganisms. [ABSTRACT FROM AUTHOR]
- Published
- 2016
38. THE USE OF STREPTOMYCES ISOLATE WITH PLANT GROWTH PROMOTING TRAITS IN THE PRODUCTION OF ENGLISH RYEGRASS.
- Author
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Stamenov, Dragana, Simonida, Đurić, Hajnal-Jafari, Timea, Jošić, Dragana, and Manojlović, Maja
- Subjects
STREPTOMYCES ,PLANT regulators ,RYEGRASSES ,AGRICULTURAL productivity ,RHIZOSPHERE - Abstract
The aim of this study was the isolation and characterization of actinomycetes (Streptomyces sp.) with PGP traits in the rhizosphere of perennial ryegrass, and monitoring the impact of their use on the parameters of the English ryegrass (Lolium perenne) yield and microbiological activity in its rhizosphere. The isolates had different growth characteristics. All three isolates produced a greater amount of indole-acetic acid (IAA) in the medium with added L-tryptophan. Isolate A3 produced siderophors and hydrogen cyanide, while isolates A1 and A2 utilized organic phosphorus. All isolates were produced cellulase, urease and gelatinase but not amylase. Isolates A1 and A3 produced lipase while A2 produced a protease. Isolate A3 was chosen for the examination of the isolate impact on plant yield. When evaluated on English Ryegrass in the field, during the first year of experiment, the isolate A3 affected positively the yield of fresh and dry mass, root and stem length. During the second year, the isolate A3 statistically significant enhanced the measured parameters over the control. In relation to Trichoderma asperellum, application of isolate A3 had a better effect on the plant yield. The number of investigated group of microorganisms and dehydrogenase activity in the inoculated variant increased in comparison with the control. [ABSTRACT FROM AUTHOR]
- Published
- 2016
39. Influence of Pseudomonasand BacillusStrains Isolated from Lolium perenneRhizospheric Soil in Vojvodina (Serbia) on Planth Growth and Soil Microbial Communities
- Author
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STAMENOV, DRAGANA R., DJURIC, SIMONIDA, HAJNAL-JAFARI, TIMEA, and ANDJELKOVIC, SNEŽANA
- Abstract
The aim of this study was the isolation of Pseudomonassp. and Bacillussp. strains from rhizospheric soil and monitoring the impact of two isolates denoted as P12 (Pseudomonassp.) and B1 (Bacillussp.) on the parameters of English ryegrass (Lolium perenne) yield and activity of the soil microbial communities. During 2012–2014, a plot experiment was set up following the randomized block system. Better effect on the plant growth was recorded with the use of Pseudomonassp. P12 isolate than with Bacillussp. B1. Positive effect on the increase in the total number of microorganisms, aminoheterotrophs and azotobacter was also achieved. Bacillussp. B1 increased only the number of actimycetes. Both isolates positively affected dehydrogenase activity (DHA).
- Published
- 2017
- Full Text
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