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182 results on '"Stabile, Anna Maria"'

4. Liposome-based nanoparticles impact on regulatory and effector phenotypes of macrophages and T cells in multiple Sclerosis patients

Author

Tredicine, Maria, Ria, Francesco, Poerio, Noemi, Lucchini, Matteo, Bianco, Assunta, De Santis, Federica, Valentini, Mariagrazia, De Arcangelis, Valeria, Rende, Mario, Stabile, Anna Maria, Pistilli, Alessandra, Camponeschi, Chiara, Nociti, Viviana, Mirabella, Massimiliano, Fraziano, Maurizio, and Di Sante, Gabriele

Published
2023
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6. The unknown and misunderstood life of Ruggero Oddi, the pioneer of biliary system physiology.

Author

Maovaz, Marco, Di Sante, Gabriele, Bartolini, Desirée, Pistilli, Alessandra, Stabile, Anna Maria, and Rende, Mario

Abstract

Ruggero Oddi was a talented scientist who initiated the modern era of biliary system physiology, not only with the anatomical discovery of the hepatopancreatic sphincter, but also with the detailed description of its spinal center and nerve regulation. However, his personal and scientific life were determined by an incredible series of unfortunate circumstances. Until now most of these events have been unknown, while the few known have produced biographies distorted by fake interpretations. The purpose of this article is to document Oddi's biography and scientific production in detail, comprehensively framing the scientific environment in which his discoveries occurred. It clears many misinterpretations about events in Oddi's life and academic career, bringing to a new light his figure as scientist in gastroenterological field. [ABSTRACT FROM AUTHOR]

Published
2024
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9. Randomised controlled trial combining vitamin E-functionalised chocolate with physical exercise to reduce the risk of protein–energy malnutrition in predementia aged people: study protocol for Choko-Age

Author

Pedrinolla, Anna, primary, Isanejad, Masoud, additional, Antognelli, Cinzia, additional, Bartolini, Desirée, additional, Borras, Consuelo, additional, Cavedon, Valentina, additional, Di Sante, Gabriele, additional, Migni, Anna, additional, Mas-Bargues, Cristina, additional, Milanese, Chiara, additional, Baschirotto, Claudia, additional, Modena, Roberto, additional, Pistilli, Alessandra, additional, Rende, Mario, additional, Schena, Federico, additional, Stabile, Anna Maria, additional, Telesa, Nicola Vincenzo, additional, Tortorella, Sara, additional, Hemmings, Kay, additional, Vina, Jose, additional, Wang, Eivind, additional, McArdle, Anne, additional, Jackson, Malcolm J, additional, Venturelli, Massimo, additional, and Galli, Francesco, additional

Published
2023
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14. A Possible Role for Nerve Growth Factor and Its Receptors in Human Sperm Pathology.

Author

Stabile, Anna Maria, Pistilli, Alessandra, Moretti, Elena, Bartolini, Desirée, Ruggirello, Mariangela, Rende, Mario, Castellini, Cesare, Mattioli, Simona, Ponchia, Rosetta, Tripodi, Sergio Antonio, and Collodel, Giulia

Subjects
NERVE growth factor, SPERMATOZOA, SEMEN
Abstract

Nerve growth factor (NGF) signalling affects spermatogenesis and mature sperm traits. In this paper, we aimed to evaluate the distribution and the role of NGF and its receptors (p75NTR and TrKA) on the reproductive apparatus (testis and epididymis) and sperm of fertile men (F) and men with different pathologies, namely varicocele (V) and urogenital infections (UGIs). We collected semen samples from 21 individuals (31–40 years old) subdivided as follows: V (n = 7), UGIs (n = 7), and F (n = 7). We submitted the semen samples to bacteriological analysis, leucocyte identification, and analysis of sperm parameters (concentration, motility, morphology, and viability). We determined the seminal plasma levels of NGF, interleukin 1β (IL-1β), and F2-isoprostanes (F2-IsoPs), and the gene and protein expression of NGF receptors on sperm. We also used immunofluorescence to examine NGF receptors on ejaculated sperm, testis, and epididymis. As expected, fertile men showed better sperm parameters as well as lower levels of NGF, F2-IsoPs, and IL-1β compared with men with infertility. Notably, in normal sperm, p75NTR and TrKA were localised throughout the entire tail. TrKA was also found in the post-acrosomal sheath. This localisation appeared different in patients with infertility: in particular, there was a strong p75NTR signal in the midpiece and the cytoplasmic residue or coiled tails of altered ejaculated sperm. In line with these findings, NGF receptors were intensely expressed in the epididymis and interstitial tissue of the testis. These data suggest the distinctive involvement of NGF and its receptors in the physiology of sperm from fertile men and men with infertility, indicating a possible role for new targeted treatment strategies. [ABSTRACT FROM AUTHOR]

Published
2023
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17. Zinc restores functionality in porcine prepubertal Sertoli cells exposed to subtoxic cadmium concentration via regulating the Nrf2 signaling pathway

Author

Mancuso, Francesca, primary, Arato, Iva, additional, Bellucci, Catia, additional, Lilli, Cinzia, additional, Eugeni, Elena, additional, Aglietti, Maria Chiara, additional, Stabile, Anna Maria, additional, Pistilli, Alessandra, additional, Brancorsini, Stefano, additional, Gaggia, Francesco, additional, Calvitti, Mario, additional, Baroni, Tiziano, additional, and Luca, Giovanni, additional

Published
2023
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19. Zinc restores functionality in porcine prepubertal Sertoli cells exposed to subtoxic cadmium concentration via regulating the Nrf2 signaling pathway

Author

Mancuso, Francesca, Arato, Iva, Bellucci, Catia, Lilli, Cinzia, Eugeni, Elena, Aglietti, Maria Chiara, Stabile, Anna Maria, Pistilli, Alessandra, Brancorsini, Stefano, Gaggia, Francesco, Calvitti, Mario, Baroni, Tiziano, and Luca, Giovanni

Subjects
Nrf2 signaling pathway, cadmium, Endocrinology, Diabetes and Metabolism, Sertoli cells, zinc, oxidative stress
Abstract

IntroductionAmong substances released into the environment by anthropogenic activities, the heavy metal cadmium (Cd) is known to induce severe testicular injury causing male subfertility/infertility. Zinc (Zn) is another heavy metal that, unlike Cd, is physiologically present in the testis, being essential for spermatogenesis. We aimed to examine the possibility that 50 µM ZnCl2 could counteract the toxic effects induced by Cd in an in vitro model of porcine prepubertal Sertoli cells (SCs) exposed to both subtoxic (5 μM) and toxic (10 μM) concentrations of CdCl2 for 48 h.Materials and MethodsApoptosis, cell cycle, and cell functionality were assessed. The gene expression of Nrf2 and its downstream antioxidant enzymes, ERK1/2, and AKT kinase signaling pathways were evaluated.Materials and ResultsWe found that Zn, in co-treatment with subtoxic and toxic Cd concentration, increased the number of metabolically active SCs compared to Cd exposure alone but restored SC functionality only in co-treatment with subtoxic Cd concentration with respect to subtoxic Cd alone. Exposure of Cd disrupted cell cycle in SCs, and Zn co-treatment was not able to counteract this effect. Cd alone induced SC death through apoptosis and necrosis in a dose-dependent manner, and co-treatment with Zn increased the pro-apoptotic effect of Cd. Subtoxic and toxic Cd exposures activated the Nrf2 signaling pathway by increasing gene expression of Nrf2 and its downstream genes (SOD, HO-1, and GSHPx). Zn co-treatment with subtoxic Cd attenuated upregulation on the Nrf2 system, while with toxic Cd, the effect was more erratic. Studying ERK1/2 and AKT pathways as a target, we found that the phosphorylation ratio of p-ERK1/2 and p-AKT was upregulated by both subtoxic and toxic Cd exposure alone and in co-treatment with Zn.DiscussionOur results suggest that Zn could counteract Cd effects by increasing the number of metabolically active SCs, fully or partially restoring their functionality by modulating Nrf2, ERK1/2, and AKT pathways. Our SC model could be useful to study the effects of early Cd exposure on immature testis, evaluating the possible protective effects of Zn.

Published
2023

22. Abnormalities of the cellular redox associated with the cytopathic and proinflammatory effects of SARS-CoV2 infection

Author

Bartolini, Désirée, primary, Giustarini, Daniela, additional, Marcantonini, Giada, additional, Rossi, Ranieri, additional, Stabile, Anna Maria, additional, Rende, Mario, additional, Gioiello, Antimo, additional, Zatini, Linda, additional, Mencacci, Antonella, additional, Cruciani, Gabriele, additional, and Galli, Francesco, additional

Published
2022
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24. The role of hypercortisolism, inflammation and modified redox on the functional loss of muscle with age: protective effects of vitamin E and cocoa polyphenols

Author

Hemmings, Kay, primary, Prokopidis, Konstantino, additional, Migni, Anna, additional, Cinzia, Antognelli, additional, Bartolini, Desirée, additional, Borrás, Consuelo, additional, Cruciani, Gabriele, additional, Di Sante, Gabriele, additional, Giada, Marcantonini, additional, Bargues, Cristina Mas, additional, Modena, Roberto, additional, Pedrinolla, Anna, additional, Piroddi, Marta, additional, Pistilli, Alessandra, additional, Rende, Mario, additional, Shena, Federico, additional, Bartolomeo, Sebastiani, additional, Stabile, Anna Maria, additional, Vincenzo, Nicola Talesa, additional, Tortorella, Sara, additional, Venturelli, Massimo, additional, Vina, Jose, additional, Wang, Eivind, additional, Zatini, Linda, additional, Galli, Francesco, additional, Isanejad, Masoud, additional, Jackson, Malcolm, additional, and McArdle, Anne, additional

Published
2022
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25. Melatonin modulates Nrf2 activity to protect porcine pre‐pubertal Sertoli cells from the abnormal H 2 O 2 generation and reductive stress effects of cadmium

Author

Bartolini, Desirée, primary, Arato, Iva, additional, Mancuso, Francesca, additional, Giustarini, Daniela, additional, Bellucci, Catia, additional, Vacca, Carmine, additional, Aglietti, Maria Chiara, additional, Stabile, Anna Maria, additional, Rossi, Ranieri, additional, Cruciani, Gabriele, additional, Rende, Mario, additional, Calafiore, Riccardo, additional, Luca, Giovanni, additional, and Galli, Francesco, additional

Published
2022
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27. The Effect of Interaction NGF/p75NTR in Sperm Cells: A Rabbit Model

Author

Castellini, Cesare, primary, Mattioli, Simona, additional, Cotozzolo, Elisa, additional, Pistilli, Alessandra, additional, Rende, Mario, additional, Bartolini, Desirée, additional, Di Sante, Gabriele, additional, Menchetti, Laura, additional, Dal Bosco, Alessandro, additional, and Stabile, Anna Maria, additional

Published
2022
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31. High molecular weight uremic retention solutes stimulate GSTP protein expression and oxidative damage of peripheral blood leukocytes

Author

Bartolini, Desirée, primary, Stabile, Anna Maria, additional, Grignano, Maria Antonietta, additional, Focaia, Riccardo, additional, Torquato, Pierangelo, additional, Di Cristina, Manlio, additional, Piro, Federica, additional, Rende, Mario, additional, Libetta, Carmelo, additional, and Galli, Francesco, additional

Published
2021
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32. SARS-CoV2 infection impairs the metabolism and redox function of cellular glutathione

Author

Bartolini, Desirée, primary, Stabile, Anna Maria, additional, Bastianelli, Sabrina, additional, Giustarini, Daniela, additional, Pierucci, Sara, additional, Busti, Chiara, additional, Vacca, Carmine, additional, Gidari, Anna, additional, Francisci, Daniela, additional, Castronari, Roberto, additional, Mencacci, Antonella, additional, Di Cristina, Manlio, additional, Focaia, Riccardo, additional, Sabbatini, Samuele, additional, Rende, Mario, additional, Gioiello, Antimo, additional, Cruciani, Gabriele, additional, Rossi, Ranieri, additional, and Galli, Francesco, additional

Published
2021
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35. The Choko-Age project: development and analytical characterization of a new chocolate product functionalized with vitamin E to combine with physical exercise in preventing malnutrion in pre-dementia eldery subjects

Author

Migni, Anna, Galli, Francesco, Antognelli, Cinzia, Bartolini, Desiree, Borras, Consuelo, Cruciani, Gabriele, Gabriele Di Sante, Hemmings, Kay, Jackson, Malcom, Mcardle, Anne, Marcantonini, Giada, Mas-Bargues, Cristina, Masoud, Pedrinolla, Anna, Modena, Roberto, Piroddi, Marta, Pistilli, Alessandra, Rende, Mario, Schena, Federico, Sebastiani, Bartolomeo, Stabile, Anna Maria, Talesa, Vincenzo Nicola, Tortorella, Sara, Venturelli, Massimo, Vina, Jose, Wang, Eivind, and Zatini, Linda

Subjects
Physiology (medical), Biochemistry
Published
2022

38. Melatonin modulates Nrf2 activity to protect porcine pre‐pubertal Sertoli cells from the abnormal H2O2 generation and reductive stress effects of cadmium.

Author

Bartolini, Desirée, Arato, Iva, Mancuso, Francesca, Giustarini, Daniela, Bellucci, Catia, Vacca, Carmine, Aglietti, Maria Chiara, Stabile, Anna Maria, Rossi, Ranieri, Cruciani, Gabriele, Rende, Mario, Calafiore, Riccardo, Luca, Giovanni, and Galli, Francesco

Subjects
SERTOLI cells, NUCLEAR factor E2 related factor, TESTIS physiology, CADMIUM, MELATONIN
Abstract

Melatonin (MLT) is a cytoprotective agent holding potential to prevent cadmium (Cd) toxicity and its impact in testicular function and fertility. In this study, we explored such potential in porcine pre‐pubertal Sertoli cells (SCs). Cd toxicity resulted in impaired SC viability and function, abnormal cellular H2O2 generation and efflux, and induction of reductive stress by the upregulation of Nrf2 expression and activity, cystine uptake and glutathione biosynthesis, glutathione‐S‐transferase P (GSTP) expression, and protein glutathionylation inhibition. Cd toxicity also stimulated the activity of cellular kinases (MAPK‐ERK1/2 and Akt) and NFkB transcription factor, and cJun expression was increased. MLT produced a potent cytoprotective effect when co‐administered with Cd to SCs; its efficacy and the molecular mechanism behind its cytoprotective function varied according to Cd concentrations. However, a significant restoration of cell viability and function, and of H2O2 levels, was observed both at 5 and 10 μM Cd. Mechanistically, these effects of MLT were associated with a significant reduction of the Cd‐induced activation of Nrf2 and GSTP expression at all Cd concentrations. CAT and MAPK‐ERK1/2 activity upregulation was associated with these effects at 5 μM Cd, whereas glutathione biosynthesis and efflux were involved at 10 μM Cd together with an increased expression of the cystine transporter xCT, of cJun and Akt and NFkB activity. MLT protects SCs from Cd toxicity reducing its H2O2 generation and reductive stress effects. A reduced activity of Nrf2 and the modulation of other molecular players of MLT signaling, provide a mechanistic rational for the cytoprotective effect of this molecule in SCs. [ABSTRACT FROM AUTHOR]

Published
2022
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39. SARS-CoV-2 Survival on Surfaces and the Effect of UV-C Light

Author

Gidari, Anna, primary, Sabbatini, Samuele, additional, Bastianelli, Sabrina, additional, Pierucci, Sara, additional, Busti, Chiara, additional, Bartolini, Desirée, additional, Stabile, Anna Maria, additional, Monari, Claudia, additional, Galli, Francesco, additional, Rende, Mario, additional, Cruciani, Gabriele, additional, and Francisci, Daniela, additional

Published
2021
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40. Alpha-Tocopherol Metabolites (The Vitamin E Metabolome) and Their Interindividual Variability during Supplementation

Author

Bartolini, Desirée, primary, Marinelli, Rita, additional, Giusepponi, Danilo, additional, Galarini, Roberta, additional, Barola, Carolina, additional, Stabile, Anna Maria, additional, Sebastiani, Bartolomeo, additional, Paoletti, Fabiola, additional, Betti, Michele, additional, Rende, Mario, additional, and Galli, Francesco, additional

Published
2021
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41. The Effect of Interaction NGF/p75 NTR in Sperm Cells: A Rabbit Model.

Author

Castellini, Cesare, Mattioli, Simona, Cotozzolo, Elisa, Pistilli, Alessandra, Rende, Mario, Bartolini, Desirée, Di Sante, Gabriele, Menchetti, Laura, Dal Bosco, Alessandro, and Stabile, Anna Maria

Subjects
NEUROTROPHIN receptors, SPERMATOZOA, NERVE growth factor, GENITALIA, CELL motility, RABBITS, FROZEN semen
Abstract

Background: Nerve Growth Factor (NGF) plays an important role in the reproductive system through its receptor's interaction (p75NTR). This paper aims to analyze the impact of NGF p75NTR in epididymal and ejaculated rabbit semen during in vitro sperm storage. Methods: Semen samples from 10 adult rabbit bucks were collected four times (n = 40) and analyzed. NGF was quantified in seminal plasma, and the basal expression of p75NTR in sperm was established (time 0). Moreover, we evaluated p75NTR, the apoptotic rates, and the main sperm parameters, at times 2–4 and 6 h with or without the administration of exogenous NGF. Results: Based on the level of p75NTR, we defined the threshold value (25.6%), and sperm were divided into High (H) and Normal (N). During sperm storage, p75NTR of H samples significantly modulated some relevant sperm parameters. Specifically, comparing H samples with N ones, we observed a reduction in motility and non-capacitated cell number, together with an increased percentage of dead and apoptotic cells. Notably, the N group showed a reduction in dead and apoptotic cells after NGF treatment. Conversely, the NGF administration on H sperm did not change either the percentage of dead cells or the apoptotic rate. Conclusion: The concentration of p75NTR on ejaculated sperm modulates many semen outcomes (motility, apoptosis, viability) through NGF interaction affecting the senescence of sperm. [ABSTRACT FROM AUTHOR]

Published
2022
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42. Effects of nicotine on porcine pre-pupertal sertoli cells: An in vitro study

Author

Marinucci, Lorella, primary, Balloni, Stefania, additional, Bellucci, Catia, additional, Lilli, Cinzia, additional, Stabile, Anna Maria, additional, Calvitti, Mario, additional, Aglietti, Maria Chiara, additional, Gambelunghe, Angela, additional, Muzi, Giacomo, additional, Rende, Mario, additional, Luca, Giovanni, additional, Mancuso, Francesca, additional, and Arato, Iva, additional

Published
2020
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43. Endoplasmic reticulum stress and NF‐kB activation in SARS‐CoV‐2 infected cells and their response to antiviral therapy.

Author

Bartolini, Desirée, Stabile, Anna Maria, Vacca, Carmine, Pistilli, Alessandra, Rende, Mario, Gioiello, Antimo, Cruciani, Gabriele, and Galli, Francesco

Subjects
*ENDOPLASMIC reticulum, *NF-kappa B, *UNFOLDED protein response, *SARS-CoV-2, *GENETIC transcription regulation
Abstract

Unfolded protein response (UPR) and endoplasmic reticulum (ER) stress are aspects of SARS‐CoV‐2‐host cell interaction with proposed role in the cytopathic and inflammatory pathogenesis of this viral infection. The role of the NF‐kB pathway in these cellular processes remains poorly characterized. When investigated in VERO‐E6 cells, SARS‐CoV‐2 infection was found to markedly stimulate NF‐kB protein expression and activity. NF‐kB activation occurs early in the infection process (6 hpi) and it is associated with increased MAPK signaling and expression of the UPR inducer IRE‐1α. These signal transduction processes characterize the cellular stress response to the virus promoting a pro‐inflammatory environment and caspase activation in the host cell. Inhibition of viral replication by the viral protease inhibitor Nelfinavir reverts all these molecular changes also stimulating c‐Jun expression, a key component of the JNK/AP‐1 pathway with important role in the IRE‐1α‐mediated transcriptional regulation of stress response genes with anti‐inflammatory and cytoprotection function. The present study demonstrates that UPR signaling and its interaction with cellular MAPKs and the NF‐kB activity are important aspects of SARS‐CoV‐2‐host cell interaction that deserve further investigation to identify more efficient therapies for this viral infection. [ABSTRACT FROM AUTHOR]

Published
2022
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44. Non-Aβ-Dependent Factors Associated with Global Cognitive and Physical Function in Alzheimer’s Disease: A Pilot Multivariate Analysis

Author

Pedrinolla, Anna, primary, Venturelli, Massimo, additional, Tamburin, Stefano, additional, Fonte, Cristina, additional, Stabile, Anna Maria, additional, Galazzo, Ilaria Boscolo, additional, Ghinassi, Barbara, additional, Venneri, Mary Anna, additional, Pizzini, Francesca Benedetta, additional, Muti, Ettore, additional, Smania, Nicola, additional, Baldassarre, Angela Di, additional, Naro, Fabio, additional, Rende, Mario, additional, and Schena, Federico, additional

Published
2019
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46. The S100B protein as a therapeutic target for multiple sclerosis processes.

Author

Di Sante, Gabriele, Camponeschi, Chiara, De Carluccio, Maria, Amadio, Susanna, Clementi, Maria Elisabetta, Sampaolese, Beatrice, Volonté, Cinzia, Stabile, Anna Maria, Bartolini, Desirée, Pistilli, Alessandra, Tredicine, Maria, Ria, Francesco, Rende, Mario, and Michetti, Fabrizio

Subjects
MULTIPLE sclerosis, PROTEINS, CALCIUM-binding proteins
Abstract

The article presents a study on S100B protein as a therapeutic target for multiple sclerosis processes. Topics include information on calcium-binding protein mainly concentrated in astrocytes; how biological fluids are recognized as a reliable, even predictive, biomarker of active neural distress; and how S100B high levels may play a promoting role in multiple sclerosis (MS).

Published
2022

50. Multiomic deconstruction of (teno)synovial tissue: application of innovative technologies for the prevention of rheumatoid arthritis.

Author

Di Sante, Gabriele, Coletto, Lavinia Agra, Di Mario, Clara, Tolusso, Barbara, Stabile, Anna Maria, Bartolini, Desirée, Pistilli, Alessandra, Kurowska-Stolarska, Mariola, Gremese, Elisa, Rende, Mario, and Alivernini, Stefano

Abstract

The article focuses on the use of innovative technologies to deconstruct (teno)synovial tissue and identify potential biomarkers for the prevention and management of rheumatoid arthritis in individuals at risk.

Published
2023

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