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The effect of exploitation and adrenergic system drugs on sexual reflexes and properties of the semen in somatically mature Polish Large White boars has been presented. At the first stage 8 males were exploited twice a week. Then, previous to semen collection, the animals were given beta-adrenolytic (Propranolol--Polfa), after 30 minutes followed by the preparation Levonor--Polfa (alpha-adrenomimetic). 15 minutes after last injection the semen was collected, this procedure having been repeated twice a week. At the second stage of the experiment the same males were exploited once a week. Like in the first stage, the animals were given the same doses of the drugs in the same sequence. 15 minutes after last injection the semen was collected once a week. At the third stage of the experiment the boars were exploited every day. Again, like in the previous stages, the animals were given the above mentioned beta-adrenolytic and alpha-adrenomimetic to collect the semen 15 minutes after last injection. In all the stages of the experiment 6-8 ejaculates were collected from each boar. Apart from preliminary assessment of concentration and morphology of spermatozoons there was determined the level of protein and activity of selected enzymes in the semen. The sexual drive as well as the time of searching reflex and ejaculation were observed. The drugs applied have been proved to be able to prolong the time of ejaculation reflex. Simultaneous application of beta-adrenolytic and alpha-adrenomimetic to animals exploited twice a week appeared to increase the number of spermatozoons in the whole ejaculate, the percentage of spermatozoons with proper movement and the activity of GGT and fucosidase. Intensive exploitation of a boar with parallel application of the preparations described bring about a gradual decrease in spermatozoons and protein in the ejaculate as well as in the activity of hyaluronidase in its plasma and in conversion to mg protein of semen plasma.

The experiments were carried out on 15 dogs and 15 cats of both sexes. All animals received ketamine intramuscularly in doses of 10 mg/kg of body weight (dogs) and 25 mg/kg (cats). After the ketamine injection operations were performed following laparotomy and then the animals were killed by exsanguination 90 min after the injection of ketamine. For histoenzymatic examinations fragments of organs were taken (liver, kidneys, spleen, lungs and heart) and histochemical examinations were done for acid phosphatase (AP), alkaline phosphatase (AIP) and non-specific esterase (NE). It was found that ketamine anaesthesia in dogs and cats causes a slight reversible damage to the liver and kidneys and increases the activity or reticuloendothelial cells in the organism.

The investigations were carried out on 6 boars aged from 10 to 12 months. Before operation, 5 ejaculates were collected from each animal. In the second stage of the investigations, the boars were subjected to the following procedures: the deferent ducts were cut and ligated, and the bulbo-urethral glands and vesicular glands were removed. Four to 28 ejaculates were collected after each operation. The ejaculates obtained at the individual stages of the experiments were subjected to the following tests: introductory assessment, concentration and morphology of spermatozoa, and biochemical investigations concerning the concentration of Mn, Co, Cd, Fe, Cr, Ni, Sr, Ag and Li. The authors proved that the secretions from the individual segments of the reproductive organ of the boar are different in respect of their biochemical composition. The secretion of the testicles and epididymides determines the concentration of Mn, Cd and Cr in the ejaculate of the animals. The level of Mn and Fe seems to be largely influenced by the secretion from the vesicular glands and deferent ducts. The secretion of the prostate and urethral glands is responsible for the level of Cr and Ni in semen plasma, and also for the concentration of Mn, Ca and Fe. The concentration of Fe in the semen of boars distinctly depends on the presence of spermatozoa and of the secretion of the bulbo-urethral glands. The AAS method failed to detect traces of Ag and Li in the ejaculates of these boars; Co was present in vestigial amounts only.