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Abstract. The rainbow trout (Oncorhynchus mykiss), an important species in Bulgarian aquaculture, is farmed in different production systems as raceways, net cages etc. The aim of the present study was to evaluate the effect of two different rearing systems on fish production, survival rate, meat chemical, mineral composition and farming economic efficiency of culticated rainbow trout (Oncorhynchus mykiss). The information from a survey on fish production and economic efficiency traits was collected from two national rainbow trout farms using the two commonest fish farming systems: in raceways, Happy Fish Ltd fish farm and in net cages, Forest Group Ltd fish farm. The average individual weight gain (g), total weight gain (kg), feed conversion ratio, production costs of 1 kg fish and the economic efficiency coefficients were determined for the two studied fish farms. The final live weight of rainbow trout in net cages was 0.30 kg vs 0.35 kg in raceways. The average individual weight gain was higher in raceways farming system (0.30 kg) than in net cages farm (0.26 kg). The mortality rate of fish in raceways was considerably lower: only 1% as compared to that in net cages (4%). The feed conversion ratio at the end of the experiment showed identical values for trout in both farms 1.01. The meat water content of fish reared in raceways and net cages was 77.46±0.65% and 74.52±0.52% respectively (P≤0.01).The protein content of fish meat was higher in fish farmed in net cages 18.84±0.29% as compared to fish cultivated in raceways 17.60±0.49% (P 0.05). Meat fat content of rainbow trout, reared in the net cage system was also considerably higher (5.26±0.30%) than the respective parameter in fish farmed in ponds (3.60±0.15%) (P≤0.001). The content of Ca and P was higher in rainbow trouts reared in raceways (138.96±1.12 mg.kg and 2844.32±39.31 mg.kg 1 -1 -1 ) compared with the values of these parameters of fish in net cages (134.46±1.96 mg.kg and 2690.31±42.81 mg.kg ) (P≤0.05). The K and Na content -1 -1 exhibited the opposite tendency with substantially higher values in trouts farmed in net cages (2658.26±48.75 mg.kg and 671.31±16.16 mg.kg ) than in fish in -1 -1 raceways (2552.90±39.93 mg.kg and 569.32±13.75 mg.kg )(P≤0.05, P≤0.001). The production costs of 1 kg rainbow trout were by 17.27% lower in the Forest Group farm than in the Happy Fish farm. The coefficient of economic efficiency in the net cage farm was by 23.44% higher than that of the raceways production system.

Abstract. Water pollution from industrial production and developing agriculture is a serious problem in aquaculture. The aim of this study was to determine the content of heavy metals Zn (zink), Pb(lead), Ni (nickel) and Cd (cadmium) in the muscles of common carp (Cyprinus carpio L.) and rainbow trout (Oncorhynchus mykiss W.), grown under different technologies. In the current study were investigated common carp (Cyprinus carpio) and rainbow trout (Oncorhynchus mykiss), cultivated in net cages, earthen ponds and raceways. The concentration of heavy metals in the muscles of fish was determined by the methods of AAS in the Scientific laboratory of the Faculty of Agriculture. The influence of different production technologies on the bioaccumulation of Zn, Pb, Ni and Cd (in the flesh of common carp and rainbow trout) was found. The Ni content in muscles was 31.25% higher in common carp, cultured at earthen ponds, compared with its content in the flesh of the fish raised in net cages. The concentration of Pd and Ni in rainbow trout, raised in raceways was higher than that determinated for rainbow trout cultivated in net cages, by 25.0% and 7.14%, respectively. The concentration of Cd and Zn of these species, grown in raceways were lower by 33.33% and 2.14%, respectively, compared with their concentration in rainbow trout, cultivated in net cages.

Background: When diagnosing and treating male infertility it is important to determine whether there are defects in the maturation process of sperm nuclei. Using nutritional supplements can improve the morphological and physiological condition of the spermatozoa. In recent years there has been an increase in the usage of supplements with different compositions which strives to determine the best combination and avoid side effects. Aim: To study the effect of PAPA nutritional supplement on the levels of DNA fragmentation of sperm cells tested with acridine orange test (single stranded DNA against double stranded DNA) in men with sub/infertility. Materials and methods: 48 men with confirmed sub/infertility underwent treatment for three months with nutritional supplement PAPA containing 9 micronutrients. The differences in levels of DNA fragmentation were determined with acridine orange test, which was conducted before and after the treatment. Results: The results were statistically significant (p