1. Sensory neuron and substance P involvement in symptoms of a zymosan-induced rat model of acute bowel inflammation
- Author
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Landau, AM, Yashpal, K, Cahill, CM, St. Louis, M, Ribeiro-da-Silva, A, and Henry, JL
- Subjects
Pharmacology and Pharmaceutical Sciences ,Biomedical and Clinical Sciences ,Neurosciences ,Clinical Sciences ,Substance Misuse ,Digestive Diseases ,Alcoholism ,Alcohol Use and Health ,Aetiology ,2.1 Biological and endogenous factors ,Acute Disease ,Animals ,Anti-Inflammatory Agents ,Non-Steroidal ,Biphenyl Compounds ,Colitis ,Colon ,Disease Models ,Animal ,Enteric Nervous System ,Ethanol ,Inflammation Mediators ,Male ,Neurogenic Inflammation ,Neurokinin-1 Receptor Antagonists ,Neurons ,Afferent ,Oligonucleotides ,Antisense ,Pain ,Posterior Horn Cells ,RNA ,Messenger ,Rats ,Rats ,Sprague-Dawley ,Receptors ,Neurokinin-1 ,Substance P ,Sympathetic Nervous System ,Zymosan ,Psychology ,Cognitive Sciences ,Neurology & Neurosurgery ,Biological psychology - Abstract
Intestinal inflammation is a painful syndrome with multiple symptoms, including chronic pain. This study examined the possible role of sensory neurons and substance P in symptoms of an animal model of acute intestinal inflammation. The model was induced by injecting ethanol and zymosan into the colon of anesthetized male rats. Three hours later, sections of the colon were stained with hematoxylin and eosin. To determine the role of substance P, 5 mg/kg of the neurokinin-1 receptor (NK-1r) antagonist, CP-96,345, or 300 microg/kg of an antisense oligonucleotide targeted at NK-1r mRNA was administered. Spinal cord sections were examined for internalization of NK-1r, as an indicator of substance P release. Sections of colon revealed infiltration of inflammatory cells following ethanol and zymosan treatment. Plasma extravasation in rats given ethanol and zymosan was significantly greater than in controls given saline only (P
- Published
- 2007