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265 results on '"Stühler, K."'

1. New definition of the “growing teratoma syndrome” based on molecular subtyping and novel biomarkers

Author

Pongratanakul, P., primary, Bremmer, F., additional, Pauls, S., additional, Poschmann, G., additional, Kresbach, C., additional, Stephan, A., additional, Parmaksiz, F., additional, Skowron, M.A., additional, Paffenholz, P., additional, Stühler, K., additional, Schüller, U., additional, Ströbel, P., additional, Heidenreich, A., additional, Che, Y., additional, Albers, P., additional, and Nettersheim, D., additional

Published
2024
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3. S153: DECRYPTING THE ROLE OF HSP90Α AND Β ISOFORMS TO OVERCOME RESISTANCE IN BCR-ABL1 LEUKEMIA

Author

Vogt, M., primary, Dienstbier, N., additional, Schliehe-Diecks, J., additional, Scharov, K., additional, Tu, J.-W., additional, Gebing, P., additional, Hogenkamp, J., additional, Picard, D., additional, Lenz, T., additional, Stühler, K., additional, Wagener, R., additional, Pandyra, A., additional, Hauer, J., additional, Fischer, U., additional, Borkhardt, A., additional, and Bhatia, S., additional

Published
2022
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4. Die Untersuchungen zum Einfluss des Tumor-Mikromilieus auf Keimzelltumoren entschl��sseln neue bona fide Faktoren der Cisplatin-Resistenz

Author

Skowron, M, Eul, K, Ludwig, GF, Wakileh, G, Stephan, A, Söhngen, C, Bister, A, Raba, K, Petzsch, P, Poschmann, G, Stühler, K, Köhrer, K, Albers, P, and Nettersheim, D

Subjects
ddc: 610, Medicine and health
Abstract

Einleitung: Die Zusammensetzung der Tumor-Mikromilieus (TM), bestehend aus der extrazellul��ren Matrix (EZM), Fibroblasten, Endothel- und Immunzellen, kann die Effizienz der konventionellen Chemotherapie beeinflussen. Auch das Ansprechen auf eine Immuntherapie wird durch die dynamischen Interaktionen [zum vollst��ndigen Text gelangen Sie ��ber die oben angegebene URL]

Published
2022
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6. Die molekulare und epigenetische Funktion des Signalmoleküls CD24 in Keimzelltumoren und dessen therapeutischer Angriff über Antikörper und NK-CAR-Zellen

Author

Skowron, M, Becker, T, Kurz, L, Jostes, S, Bremmer, F, Fronhoffs, F, Funke, K, Wakileh, G, Müller, M, Burmeister, A, Lenz, T, Stefanski, A, Stühler, K, Petzsch, P, Köhrer, K, Altevogt, P, Albers, P, Kristiansen, G, Schorle, H, Nettersheim, D, Skowron, M, Becker, T, Kurz, L, Jostes, S, Bremmer, F, Fronhoffs, F, Funke, K, Wakileh, G, Müller, M, Burmeister, A, Lenz, T, Stefanski, A, Stühler, K, Petzsch, P, Köhrer, K, Altevogt, P, Albers, P, Kristiansen, G, Schorle, H, and Nettersheim, D

Published
2022

7. The molecular and epigenetic function of the signaling molecule CD24 in germ cell tumors and its suitability as a therapeutic target using antibodies and NK-CAR cells

Author

Skowron, M.A., primary, Thomas, D.J., additional, Becker, T.K., additional, Kurz, L., additional, Jostes, S., additional, Bremmer, F., additional, Fronhoffs, F., additional, Funke, K., additional, Wakileh, G.A., additional, Müller, M.R., additional, Burmeister, A., additional, Lenz, T., additional, Stefanski, A., additional, Stühler, K., additional, Petzsch, P., additional, Köhrer, K., additional, Altevogt, P., additional, Albers, P., additional, Kristiansen, G., additional, Schorle, H., additional, and Nettersheim, D., additional

Published
2022
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8. Studying the interaction of germ cell tumors with the microenvironment reveals novel factors involved in cisplatin resistance

Author

Skowron, M.A., primary, Eul, K., additional, Stephan, A., additional, Wakileh, G.A., additional, Ludwig, G.F., additional, Söhngen, C., additional, Bister, A., additional, Raba, K., additional, Petzsch, P., additional, Poschmann, G., additional, Stühler, K., additional, Köhrer, K., additional, Albers, P., additional, and Nettersheim, D., additional

Published
2022
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14. Impaired integrin α5/β1‐mediated hepatocyte growth factor release by stellate cells of the aged liver

Author

Rohn, F., Kordes, C., Buschmann, T., Reichert, D., Wammers, M., Poschmann, G., Stühler, K., Benk, A., Geiger, F., Spatz, J., and Häussinger, D.

Subjects
Male, Hepatocyte Growth Factor, aging, Original Articles, mechanobiology, Rats, Liver, integrins, Animals, Original Article, hepatic stellate cells, Rats, Wistar, laminins, Cells, Cultured, Cellular Senescence, Integrin alpha5beta1
Abstract

Hepatic blood flow and sinusoidal endothelial fenestration decrease during aging. Consequently, fluid mechanical forces are reduced in the space of Disse where hepatic stellate cells (HSC) have their niche. We provide evidence that integrin α5/β1 is an important mechanosensor in HSC involved in shear stress‐induced release of hepatocyte growth factor (HGF), an essential inductor of liver regeneration which is impaired during aging. The expression of the integrin subunits α5 and β1 decreases in liver and HSC from aged rats. CRISPR/Cas9‐mediated integrin α5 and β1 knockouts in isolated HSC lead to lowered HGF release and impaired cellular adhesion. Fluid mechanical forces increase integrin α5 and laminin gene expression whereas integrin β1 remains unaffected. In the aged liver, laminin β2 and γ1 protein chains as components of laminin‐521 are lowered. The integrin α5 knockout in HSC reduces laminin expression via mechanosensory mechanisms. Culture of HSC on nanostructured surfaces functionalized with laminin‐521 enhances Hgf expression in HSC, demonstrating that these ECM proteins are critically involved in HSC function. During aging, HSC acquire a senescence‐associated secretory phenotype and lower their growth factor expression essential for tissue repair. Our findings suggest that impaired mechanosensing via integrin α5/β1 in HSC contributes to age‐related reduction of ECM and HGF release that could affect liver regeneration., Hepatic blood flow and sinusoidal endothelial fenestration decrease during aging. Consequently, fluid mechanical forces are reduced in the space of Disse where hepatic stellate cells (HSC) reside. During aging, hepatocyte growth factor (HGF), integrins, and extracellular matrix proteins are significantly reduced in the rat liver and in HSC. The release of HGF by HSC is triggered by mechanical forces via an integrin α5/β1‐mediated mechanism. Thus, less HGF is provided by HSC to support regenerative processes of the aged liver.

Published
2020

19. Aging impairs hepatic stellate cell functions

Author

Kordes, C, additional, Rohn, F, additional, Buschmann, T, additional, Reichert, D, additional, Wammers, M, additional, Poschmann, G, additional, Stühler, K, additional, Benk, AS, additional, Geiger, F, additional, Spatz, JP, additional, and Häussinger, D, additional

Published
2021
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21. A0549 - The molecular and epigenetic function of the signaling molecule CD24 in germ cell tumors and its suitability as a therapeutic target using antibodies and NK-CAR cells

Author

Skowron, M.A., Thomas, D.J., Becker, T.K., Kurz, L., Jostes, S., Bremmer, F., Fronhoffs, F., Funke, K., Wakileh, G.A., Müller, M.R., Burmeister, A., Lenz, T., Stefanski, A., Stühler, K., Petzsch, P., Köhrer, K., Altevogt, P., Albers, P., Kristiansen, G., Schorle, H., and Nettersheim, D.

Published
2022
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22. Quantitative insights into the cyanobacterial cell economy

Author

Zavřel, T, Faizi, M, Loureiro, C, Poschmann, G, Stühler, K, Sinetova, M, Zorina, A, Steuer, R, Červený, J, Zavřel, T, Faizi, M, Loureiro, C, Poschmann, G, Stühler, K, Sinetova, M, Zorina, A, Steuer, R, and Červený, J

Abstract

© Zavřel et al. Phototrophic microorganisms are promising resources for green biotechnology. Compared to heterotrophic microorganisms, however, the cellular economy of phototrophic growth is still insufficiently understood. We provide a quantitative analysis of light-limited, light-saturated, and light-inhibited growth of the cyanobacterium Synechocystis sp. PCC 6803 using a reproducible cultivation setup. We report key physiological parameters, including growth rate, cell size, and photosynthetic activity over a wide range of light intensities. Intracellular proteins were quantified to monitor proteome allocation as a function of growth rate. Among other physiological acclimations, we identify an upregulation of the translational machinery and downregulation of light harvesting components with increasing light intensity and growth rate. The resulting growth laws are discussed in the context of a coarse-grained model of phototrophic growth and available data obtained by a comprehensive literature search. Our insights into quantitative aspects of cyanobacterial acclimations to different growth rates have implications to understand and optimize photosynthetic productivity.

Published
2019

25. Identification of interaction networks of mutant and wild-type IDH1 in glioma cell lines

Author

Overbeck, N, Stefanski, A, Knühmann, M, Knobbe-Thomsen, CB, and Stühler, K

Subjects
ddc: 610, 610 Medical sciences, Medicine
Abstract

Diffuse gliomas are the most frequent primary brain tumors and have an unpredictable rate of progression. It has been shown that malignant transformation of glial progenitor cells is associated with a number of genetic events, including p53 mutations, 1p/19q codeletion/translocation, as well[for full text, please go to the a.m. URL], Joint-Meeting of the German Society for Neuropathology and Neuroanatomy (DGNN) and the Scandinavian Neuropathological Society (SNS)

Published
2016
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29. MicroRNA-138 promotes acquired alkylator resistance in glioblastoma by targeting the Bcl-2-interacting mediator BIM

Author

Stojcheva, Nina, Schechtmann, G, Sass, S, Roth, P, Florea, A M, Stefanski, Artur; https://orcid.org/0000-0002-5412-1014, Stühler, K, Wolter, M, Müller, N S, Theis, F J, Weller, M, Reifenberger, G, Happold, C, Stojcheva, Nina, Schechtmann, G, Sass, S, Roth, P, Florea, A M, Stefanski, Artur; https://orcid.org/0000-0002-5412-1014, Stühler, K, Wolter, M, Müller, N S, Theis, F J, Weller, M, Reifenberger, G, and Happold, C

Abstract

Glioblastoma is the most aggressive brain tumor in adults with a median survival below 12 months in population-based studies. The main reason for tumor recurrence and progression is constitutive or acquired resistance to the standard of care of surgical resection followed by radiotherapy with concomitant and adjuvant temozolomide (TMZ/RT→TMZ). Here, we investigated the role of microRNA (miRNA) alterations as mediators of alkylator resistance in glioblastoma cells. Using microarray-based miRNA expression profiling of parental and TMZ-resistant cultures of three human glioma cell lines, we identified a set of differentially expressed miRNA candidates. From these, we selected miR-138 for further functional analyses as this miRNA was not only upregulated in TMZ-resistant versus parental cells, but also showed increased expression in vivo in recurrent glioblastoma tissue samples after TMZ/RT→TMZ treatment. Transient transfection of miR-138 mimics in glioma cells with low basal miR-138 expression increased glioma cell proliferation. Moreover, miR-138 overexpression increased TMZ resistance in long-term glioblastoma cell lines and glioma initiating cell cultures. The apoptosis regulator BIM was identified as a direct target of miR-138, and its silencing mediated the induced TMZ resistance phenotype. Altered sensitivity to apoptosis played only a minor role in this resistance mechanism. Instead, we identified the induction of autophagy to be regulated downstream of the miR-138/BIM axis and to promote cell survival following TMZ exposure. Our data thus define miR-138 as a glioblastoma cell survival-promoting miRNA associated with resistance to TMZ therapy in vitro and with tumor progression in vivo.

Published
2016

32. Proteome-wide analysis reveals an age-associated cellular phenotype of in situ aged human fibroblasts

Author

Waldera-Lupa, D.M., Kalfalah, F., Florea, A.M., Sass, S., Kruse, F.E., Rieder, V., Tigges, J., Fritsche, E., Krutmann, J., Busch, H., Boerries, M., Meyer, H.E., Boege, F., Theis, F.J., Reifenberger, G., and Stühler, K.

Abstract

We analyzed anex vivo model of in situ aged human dermal fibroblasts, obtained from 15 adult healthy donors from three different age groups using an unbiased quantitative proteome-wide approach applying label-free mass spectrometry. Thereby, we identified 2409 proteins, including 43 proteins with an age-associated abundance change. Most of the differentially abundant proteins have not been described in the context of fibroblasts' aging before, but the deduced biological processes confirmed known hallmarks of aging and led to a consistent picture of eight biological categories involved in fibroblast aging, namely proteostasis, cell cycle and proliferation, development and differentiation, cell death, cell organization and cytoskeleton, response to stress, cell communication and signal transduction, as well as RNA metabolism and translation. The exhaustive analysis of protein and mRNA data revealed that 77 % of the age-associated proteins were not linked to expression changes of the corresponding transcripts. This is in line with an associated miRNA study and led us to the conclusion that most of the age-associated alterations detected at the proteome level are likely caused post-transcriptionally rather than by differential gene expression. In summary, our findings led to the characterization of novel proteins potentially associated with fibroblast aging and revealed that primary cultures of in situ aged fibroblasts are characterized by moderate age-related proteomic changes comprising the multifactorial process of aging.

Published
2014

33. Misassembly of full-length Disrupted-in-Schizophrenia 1 protein is linked to altered dopamine homeostasis and behavioral deficits

Author

Trossbach, S V, primary, Bader, V, additional, Hecher, L, additional, Pum, M E, additional, Masoud, S T, additional, Prikulis, I, additional, Schäble, S, additional, de Souza Silva, M A, additional, Su, P, additional, Boulat, B, additional, Chwiesko, C, additional, Poschmann, G, additional, Stühler, K, additional, Lohr, K M, additional, Stout, K A, additional, Oskamp, A, additional, Godsave, S F, additional, Müller-Schiffmann, A, additional, Bilzer, T, additional, Steiner, H, additional, Peters, P J, additional, Bauer, A, additional, Sauvage, M, additional, Ramsey, A J, additional, Miller, G W, additional, Liu, F, additional, Seeman, P, additional, Brandon, N J, additional, Huston, J P, additional, and Korth, C, additional

Published
2016
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38. Detection of novel biomarkers of liver cirrhosis by high performance proteomics

Author

Meyer, Helmut E., Mölleken, C., Sitek, B., Henkel, C., Poschmann, G., Sipos, B., Wiese, S, Warscheid, B., Broelcsh, C., Reiser, M., Friedman, S.L., Tornoe, I., Schlosser, A., Schmiegel, W., Holmskov, V., and Stühler, K.

Subjects
Sistemas biológicos, Biotecnología, Proteómica
Abstract

Comunicaciones a congresos

Published
2009

42. How stemlike are sphere cultures from long-term cancer cell lines? Lessons from mouse glioma models

Author

Ahmad, M, Frei, K, Willscher, E, Stefanski, A, Kaulich, K, Roth, P, Stühler, K, Reifenberger, G, Binder, H, Weller, M, Ahmad, M, Frei, K, Willscher, E, Stefanski, A, Kaulich, K, Roth, P, Stühler, K, Reifenberger, G, Binder, H, and Weller, M

Abstract

Cancer stem cells may mediate therapy resistance and recurrence in various types of cancer, including glioblastoma. Cancer stemlike cells can be isolated from long-term cancer cell lines, including glioma lines. Using sphere formation as a model for cancer cell stemness in vitro, we derived sphere cultures from SMA-497, SMA-540, SMA-560, and GL-261 glioma cells. Gene expression and proteomics profiling demonstrated that sphere cultures uniformly showed an elevated expression of stemness-associated genes, notably including CD44. Differences in neural lineage marker expression between nonsphere and sphere cultures were heterogeneous except for a uniform reduction of β-III-tubulin in sphere cultures. All sphere cultures showed slower growth. Self-renewal capacity was influenced by medium conditions but not nonsphere versus sphere culture phenotype. Sphere cultures were more resistant to irradiation, whereas both nonsphere and sphere cultures were highly resistant to temozolomide. Nonsphere cells formed more aggressive tumors in syngeneic mice than sphere cells in all models except SMA-560. There were no major differences in vascularization or infiltration by T cells or microglia/macrophages between nonsphere and sphere cell-derived tumors implanted in syngeneic hosts. Together, these data indicate that mouse glioma cell lines may be induced in vitro to form spheres that acquire features of stemness, but they do not exhibit a uniform biologic phenotype, thereby challenging the view that they represent a superior model system.

Published
2014

47. Downregulation of PRDX1 by promoter hypermethylation is frequent in 1p/19q-deleted oligodendroglial tumours and increases radio- and chemosensitivity of Hs683 glioma cells in vitro

Author

Dittmann, L M, primary, Danner, A, additional, Gronych, J, additional, Wolter, M, additional, Stühler, K, additional, Grzendowski, M, additional, Becker, N, additional, Bageritz, J, additional, Goidts, V, additional, Toedt, G, additional, Felsberg, J, additional, Sabel, M C, additional, Barbus, S, additional, Reifenberger, G, additional, Lichter, P, additional, and Tews, B, additional

Published
2011
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