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186 results on '"Srinivas, Sangly P."'

6. Transcorneal Kinetics of Topical Drugs and Nanoparticles

Author

Srinivas, Sangly P., Chaiyasan, Wanachat, Burli, Anirudh, Guidoboni, Giovanna, Sacco, Riccardo, Anand, Abhishek, Siraj, Amreen H., Daima, Hemant Kumar, Kompella, Uday B., Perrie, Yvonne, Series Editor, Neervannan, Seshadri, editor, and Kompella, Uday B., editor

Published
2021
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10. Transcorneal Kinetics of Topical Drugs and Nanoparticles

Author

Srinivas, Sangly P., primary, Chaiyasan, Wanachat, additional, Burli, Anirudh, additional, Guidoboni, Giovanna, additional, Sacco, Riccardo, additional, Anand, Abhishek, additional, Siraj, Amreen H., additional, Daima, Hemant Kumar, additional, and Kompella, Uday B., additional

Published
2021
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14. Anterior chamber inflammation grading methods: A critical review

Author

Oo, Hnin Hnin, primary, Mohan, Madhuvanthi, additional, Song, Wenjun, additional, Rojas-Carabali, William, additional, Tsui, Edmund, additional, de-la-Torre, Alejandra, additional, Cifuentes-González, Carlos, additional, Rousselot, Andres, additional, Srinivas, Sangly P., additional, Aslam, Tariq, additional, Gupta, Vishali, additional, and Agrawal, Rupesh, additional

Published
2023
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41. Efficacy of Natural Blue Dye from Butterfly Pea Flower for Anterior Capsule Staining during Cataract Surgery: An Ex Vivo Study.

Author

THONGSUK, WANACHAT, KHOTCHARRAT, ROSSUKON, PAENSUWAN, PUSSADEE, PEJCHANG, DARAWAN, and SRINIVAS, SANGLY P.

Subjects
NATURAL dyes & dyeing, CENTROSEMA, CATARACT surgery, DEIONIZATION of water, CELL-mediated cytotoxicity
Abstract

This study aims to investigate the efficacy of natural blue dye from the butterfly pea flower to be a candidate blue vital dye for staining the anterior capsule of the crystalline lens. The blue dye was extracted from the butterfly pea flower with warm deionized water and sterilized by filtration. The stability of the extracted blue dye solution was performed for 12 months. The efficacy of staining the anterior capsule of the lens was performed using a cataract-induced porcine eyeball ex vivo model. Cataract was induced either by microwave radiation or injection of solvents. Histological examination of the corneal endothelial cells was evaluated after exposure to the extracted blue dye for 5 min. For the present study, the blue dye of the butterfly pea flowers could be successfully extracted. The dye was found to be stable at 12 months when stored at 4oC in the dark and was appeared to be stable even when stored at room temperatures with high concentration. Both microwave radiation and solvents injection led to a loss of lens transparency. The former resulted in a whitish haze while the latter led to a yellowish appearance. The capsule showed significant blue-stains when exposed to an aqueous solution of the blue dye (500 mg/ml). Histology revealed normal corneal endothelium structure, indicate that there is no notable cytotoxicity after blue dye staining. A blue dye from the butterfly pea flowers, extracted into an aqueous solution, exhibited potential for staining the anterior capsule of the crystalline lens. [ABSTRACT FROM AUTHOR]

Published
2021
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44. Elevated cAMP opposes (TNF-α)-induced loss in the barrier integrity of corneal endothelium

Author

Shivanna, Mahesh and Srinivas, Sangly P.

Subjects
Adenosine, Tumor Necrosis Factor-alpha, Colforsin, Endothelium, Corneal, Membrane Proteins, Epithelial Cells, Actomyosin, Adenosine-5'-(N-ethylcarboxamide), Cadherins, Phosphoproteins, Microtubules, p38 Mitogen-Activated Protein Kinases, Permeability, Enzyme Activation, Intercellular Junctions, Blood-Retinal Barrier, Cyclic AMP, Electric Impedance, Zonula Occludens-1 Protein, Animals, Cattle, Rolipram, Research Article
Abstract

Purpose Elevated cyclic adenosine monophosphate (cAMP) enhances the barrier integrity of the corneal endothelium and thereby facilitates stromal hydration control, which is necessary for corneal transparency. This study investigates whether elevated cAMP is effective against the tumor necrosis factor-alpha (TNF-α)-induced loss of barrier integrity in monolayers of bovine corneal endothelial cells (BCEC). Methods BCEC in primary culture were used for the study. Trans-endothelial electrical resistance (TER), a measure of barrier integrity, was determined by electrical cell-substrate impedance sensing. The changes were also ascertained by measuring paracellular permeability to fluorescein isothiocyanate (FITC)-dextran (10 kDa) across cells grown on porous culture inserts, and by immunofluorescence imaging of the apical junctional complex (AJC). The activation of p38 MAP kinase was assessed using western blotting. Results Co-treatment with forskolin, which activates adenylate cyclase, and rolipram, which inhibits cAMP-dependent phosphodiesterase PDE4, reduced the TNF-α-induced increase in the flux of FITC-dextran. Similar co-treatment also prevented the TNF-α-induced disorganization of zona occludens-1 (ZO-1) and cadherins at the AJC. Co-treatment, as well pre-treatment, with forskolin plus rolipram prevented the TNF-α-induced decrease in TER. The influence of the agents was significant after 12 h of exposure to the cytokine. This effect was also mimicked by A2B agonists, adenosine and 5′-N-ethylcarboxamidoadenosine (NECA), which are known to mobilize cAMP in BCEC. Elevated cAMP also inhibited the cytokine-induced activation of p38 MAP kinase, and further blocked the disassembly of microtubules as well as the disruption of the PAMR (peri-junctional actomyosin ring) at the AJC. Conclusions These results suggest that elevated cAMP opposes the TNF-α-induced loss in barrier integrity of the corneal endothelium. This effect follows inhibition of the cytokine-induced activation of p38 MAP kinase and its downstream signaling involved in the disruption of AJC and PAMR, as well as the disassembly of microtubules.

Published
2010

45. Targeted Intraceptor Nanoparticle Therapy Reduces Angiogenesis and Fibrosis in Primate and Murine Macular Degeneration

Author

Luo, Ling, primary, Zhang, Xiaohui, additional, Hirano, Yoshio, additional, Tyagi, Puneet, additional, Barabás, Péter, additional, Uehara, Hironori, additional, Miya, Tadashi R., additional, Singh, Nirbhai, additional, Archer, Bonnie, additional, Qazi, Yureeda, additional, Jackman, Kyle, additional, Das, Subrata K., additional, Olsen, Thomas, additional, Chennamaneni, Srinivas R., additional, Stagg, Brian C., additional, Ahmed, Faisal, additional, Emerson, Lyska, additional, Zygmunt, Kristen, additional, Whitaker, Ross, additional, Mamalis, Christina, additional, Huang, Wei, additional, Gao, Guangping, additional, Srinivas, Sangly P., additional, Krizaj, David, additional, Baffi, Judit, additional, Ambati, Jayakrishna, additional, Kompella, Uday B., additional, and Ambati, Balamurali K., additional

Published
2013
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