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Perkecambahan biji merupakan fase kritis yang menentukan kelangsungan hidup dan toleransi tanaman pada lingkungan tercemar logam berat. Krom heksavalen (Cr6+) adalah salah satu logam berat yang bersifat toksik bagi tumbuhan. Tujuan penelitian mengetahui efek Cr6+ terhadap perkecambahan biji dan pertumbuhan kecambah Tagetes erecta. Uji perkecambahan biji dan pertumbuhan kecambah dilakukan secara eksperimental menggunakan rancangan acak lengkap dengan 4 perlakuan konsentrasi Cr6+ (K2CrO4) meliputi 0 (kontrol), 5, 25, dan 50 mg/L. Jumlah biji berkecambah diamati setiap hari selama 10 hari dan digunakan untuk menentukan persentase perkecambahan dan indeks vigor kecambah. Pertumbuhan kecambah ditentukan berdasarkan panjang radikula dan panjang epikotil, dan bobot kering kecambah pada akhir penelitian. Cr6+ mempengaruhi secara signifikan perkecambahan dan pertumbuhan kecambah T. erecta. Konsentrasi Cr6+ sebesar 5, 25 dan 50 mg/L menurunkan secara signifikan (p

Euphorbiaceae is the most diverse angiosperm plant with one of the largest number of species widely used in various industries. Exploration and inventory of the Euphorbiaceae family has not been maximized, including in Salatiga City. Its great potential requires an initial step, namely plant breeding, but knowledge of its phenetic relationships is needed so that plant breeding produces superior individuals. In addition to morphological characters, chemical properties such as milk clotting activity are important in understanding the phenetic relationships. This study aims to analyze the phenetic relationships of Euphorbiaceae members in Salatiga City based on their morphological and chemical characteristics as well as from the milk clotting activity. Twenty five individual members of the Euphorbiaceae were analyzed for their morphological and chemical characteristics, and 30 characters were obtained. The results of the study using Cluster Analysis method showed that 25 individuals formed cluster A which was divided into 2 parts (clusters C and D) and cluster B formed cluster G (consists of individuals of Codiaeum variegatum (L.) Bl.). The closest phenetic relationships between Jatropha multifida Sidomukti C09 and Jatropha multifida Sidorejo C18 with a dissimilarity index of 0.03% or the equation 99.97%. The most distant phenetic relationships in Codiaeum variegatum (L.) Bl. Sidorejo C11 and Codiaeum variegatum (L.) Bl. Sidomukti C03 with a dissimilarity index of 19.13% or the equivalent percentage of 80.87%. The genus Euphorbia formed cluster F. The genus Manihot and Jatropha formed cluster C, the genus Codiaeum formed clusters E and B. All individuals, except Codiaeum variegatum (L.) Bl. Sidorejo C11, can coagulate milk so that it has potential as a protease enzyme in various industries. The highest protease enzyme activity value was in Codiaeum variegatum (L.) Bl. Sidorejo C11 (15.84 U/mg) and the lowest was in Euphorbia tirucalli Sidomukti C07 (2.34 U/mg).

The introduction of coronavirus disease (COVID-19) into the human population represents a tremendous medical and economical crisis. The immune system plays a central role in protecting against this novel virus. This activity aimed to empower young generation using participatory learning and action approach to improve their knowledge about medicinal plants that grow in Indonesia and whose anti-inflammatory, antioxidant, immunomodulatory, and antiviral properties. Organoleptic test results showed that Kunyit Asam (consisted of turmeric and ginger) and Tealang (consisted of butterfly pea and lemongrass) were highly favored by panelists. Our results provide valuable information on the medicinal plants used as an immunity booster within the studied community.

Artemisia cina is a wild herbaceous plant that has secondary metabolites as a source of antioxidants. The addition of mycorrhizal species Glomus mosseae can increase the antioxidant activity of plants Artemisia cina. This study aims to determine the effect of mycorrhizal Glomus mosseae on the growth and content of antioxidant compounds in Artemisia cina. The study used a completely randomized design (CRD), with four levels of treatment, namely: media without G. mosseae (control), media with G. mosseae 5,10 and 15 g with 5 replications for each treatment. Parameters observed were plant height, number of leaves, 5th leaf length and width, wet and dry weight, root length, percentage of mycorrhizal colonization, chlorophyll content, carotenoids, flavonoids, phenols were also observed. Antioxidant activity was determined by the DPPH method. The results showed that the treatment Glomus mosseae with a dose of 15 g was significantly different from the control on plant growth parameters, number of leaves, root length, wet weight and dry weight of plants, and percentage of mycorrhizal colonization in plants. Treatment of Glomus mosseae 15 g on the test results of antioxidant compounds also showed the highest content of chlorophyll, carotenoids, phenols and flavonoids from the statistical test results found in the 15 g treatment, respectively, namely 6,405 g/ml; 0.766 g/ml; 70.9 g/ml; and 335 g/ml had a significant difference to the control treatment. While the results of the antioxidant activity test using the DPPH method showed that the control treatment had the highest antioxidant activity with an ICvalue50 of 9,494 g/ml. The results showed that the mycorrhizal species of G. mosseae increased the growth and content of antioxidant compounds inplants A. cina.

Many studies show the antioxidant ability of the parasite plant. This study aims to determine the capacity of Dendrophthoe pentandra (L.) Miq and Scurrula ferruginea stem from Syzygium aqueum to use as a natural antioxidant source. The sample was taken from the host Syzygium aqueum. The sampels were exraction with ethanol using maceration. The parameters determined spectrophotometrically using specific reagents that were antioxidant capacity using 1,1-diphenyl-2-picrylhydrazyl, phenolics compounds using Folin-Ciocalteu, flavonoids using AlCl3, ascorbic acid using sulfosalicylic acid, while chlorophyll and carotenoid using dimethyl sulfoxide. The result of D. pentandra (L.) Miq and S. ferruginea extract showed that IC50 were 15.09±1.02 and 20.53±1.77 mg/ml, content of phenolics compounds were 557.45±19.77 and 433.99±20.89 mg GAE/ml extract, flavonoids were 9.72±0.23 and 3.02±0.71 mg QE/ml extract, chlorophylls-a were 2.67± 0.15 and 2.77±0,08 mg/g extract, chlorophylls-b were 3.34±0.06 and 3.44±0.04 mg/g extract, total chlorophylls were 6.01±0.21 mg/g extract, carotenoids were 1.71±0.01 and 2.10±0.01 mg/g extract respectively. The stems of both D. pentandra (L.) Miq and S. ferruginea from Syzygium aqueum have very strong antioxidant abilities so that they are potential to use as a source of natural antioxidants. The compounds support the antioxidant activity were phenolic, flavonoids, and ascorbic acids. The results of this study will open up opportunities to obtain new sources of antioxidants that can be utilized in human health management.

Meniran ( Phyllanthus niruri ), patikan kebo ( Euphorbia hirta ), and benalu ( Loranthus sp.) have often been used by people as medicinal plants. This research aimed to measure the levels of flavonoids, phenolics, and ascorbic acid compounds, as well as the Free Radical Scavenging (FRS) activity of ethyl acetate extract from the mixture of P. niruri , E. hirta , and Loranthus sp. The FRS activity was measured with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The level of compounds was measured by using the spectrophotometry method with specific reagents. The result of the FRS activity in ethyl acetate extract from the mixture of P. niruri , E. hirta , and Loranthus sp. varied depending on its dose. The measure of FRS in the P. niruri : E. hirta : Loranthus sp 0:0:1 (K-OOL) composition showed a strong result with a value of IC 50 97.2 ± 2.1 ppm, while in the 0:0.5:0.5 (K-OEL) composition it was moderate with a value of IC 50 147.6 ± 6.5 ppm. The other compositions showed weak and inactive results. The K-OOL composition had the highest flavonoid and phenolic content that were 298.8±0.00 mg QE/g extract and 141.5±2.85 mg GAE/g extract respectively. The composition with the highest ascorbic acid content (298.8±0.00 mg/g extract) was K-OEO composition. So far, research on medicinal plants is still limited to one type of plant. The combination of several types of plants in several formulations allows obtaining a composition that can produce maximum antioxidant capacity. Therefore, this research is expected to produce a combination formulation of various types of medicinal plants that have the K-OOL composition very strong antioxidant activity and can be used as herbal medicines.

Meitiniarti VI, Putri EK, Runtu AE, Nugroho RA, Kasmiyati S. 2022. Isolation and identification of Cr-resistant bacteria from the rhizosphere of Tagetes sp. and their ability to reduce Cr(VI). Biodiversitas 23: 4118-4124. Bioremediation involving the interaction of plants with microorganisms is a technique for improving the environment polluted with chromium hexavalent [Cr(VI)]. Marigolds (Tagetes sp.) are known to be a hyperaccumulator plant for Cr(VI) and there are microbes in its rhizosphere that enable the plant tolerant to heavy metals. The aims of this study were to isolate and identify indigenous rhizobacteria resistant to Cr(VI) from Tagetes sp. and to test the ability of these bacterial isolates to reduce Cr(VI). Three isolates of Cr(VI) resistant bacteria were obtained in this study, namely Micrococcus luteus RT-9, Stenotrophomonas maltophilia RT-12, and Brevundimonas sp. RT-23. M. luteus RT-9 and S. maltophilia RT-12 which were resistant to Cr(VI) 100 mg L-1 and their mixture were tested for their ability to reduce Cr(VI) in a liquid medium. Microbacterium sp. SpR3 obtained by the previous study was used as a reference. The results showed that the isolate of S. maltophilia RT-12 had a higher ability (2.2 mg L-1 h-1) than M. luteus RT-9 (0.9 mg L-1 h-1). S. maltophilia RT-12 had the ability to reduce Cr(VI) similar to Microbacterium sp. SpR3. The mixed inoculation of M. luteus RT-9, S. maltophilia RT-12, and Microbacterium sp. SpR3 did not show a difference in the reduction ability of Cr (VI) compared to pure cultures.

The medicinal plant-derived bioactive compounds have a potential for many biological activities, including antimicrobial activity. Artemisia cina is a medicinal plant from the Compositae family with the potential of having antitumor, antifungal, and antibacterial activity. This study aimed to determine the antibacterial activity and the flavonoid content of A. Cina ’s ethyl acetate extract. Plants samples were extracted by ethyl acetate maceration method. Antibacterial activity was tested against Gram-negative bacteria ( Escherichia coli and Pseudomonas aeruginosa ) and Gram-positive bacteria ( Bacillus subtilis and Staphylococcus aureus ) by a disk diffusion method using 25, 50, and 100 mg/l extract concentrations. The flavonoid contents (quercetin and kaempferol) were measured using High-Performance Liquid Chromatography. The extracts of diploid and polyploid A. cina displayed some antibacterial activity, with the Gram-negative bacteria being more resistant than the Gram-positive counterpart. However, no significant difference was observed between the diploid and polyploid extracts. As for the flavonoid content, the highest quercetin content (0.5501 mg/ml) was found in the polyploid A. cina (J), while the highest kaempferol content (0.5818 mg/ml) was observed in the diploid A. cina (KJT). Although A. cina is widely grown in Indonesia, compared to other Artemisia species, A. cina has not been widely studied, especially its antibacterial potential and in related to its flavonoid content and the use of ethyl acetate as the extraction solvent.  This study reveals the potential of A. cina as a natural antibacterial agent.Â

Agricultural activity can’t be separated from synthetic herbicide. Some synthetic herbicide use metsulfuron methyl as active ingredient that works to change chemical materials in weed’s tissues so able to exterminate the tissue or disordering physiological system that needed for survivability of weeds. Because of that effect a question arises whether this compound also can affect non target organisms like periphyton algae. Periphyton is a group of microscopic organisms that grows attached to surface of objects or water plants, not penetrate the substrate, staying or moving on the substrate surfaces. One of main compilers of periphyton community is algae. Periphyton algae in water has significant role to determining primary productivity and as bioindicator of water quality. Some research that has been done shows if some active ingredient in particular concentration significantly affects toward primary productivity of periphyton algae and able to inhibit growth of certain algae species. This research was conducted to find out effects of metsulfuron methyl herbicide on periphyton algae community structure. Parameters measured are concentration of chlorophyll a, density, richness, diversity, evenness, and domination indices also periphyton algae species identification. Research conducted in laboratory scale use samples taken from Rawa Pening Lakes, Semarang District, Central Java. Observation time is 21 days with herbicide exposure at 0, 0.05, 0.1, 0.2, and 0.4 mg/L. Results show that periphyton algae community structure more impacted by exposure period of herbicide actve ingredient metsulphuron methyl than concentration that used in research. Keywords: Periphyton Algae, Metsulfuron Methyl, Community Structure

Rennet, a milk coagulant exhibiting proteolytic activity, is a crucial component in cheese industries. Its price and availability have discouraged the growth of some small scale cheese industries. Therefore, an alternative for rennet will be beneficial for the industries. Among other sources, plant proteases offer some advantages as rennet alternatives. This study aimed to investigate the potential of plant proteases obtained from the latex as potential rennet alternatives. A total of six plants from the genus Ficus  and Artocarpus  were screened for their proteolytic activity and milk coagulating ability. The screening indicated that all six tested plants displayed proteolytic activity at various levels, but only Ficus benjamina  and Artocarpus heterophyllus  produced a firm milk curd. Hence, both F. benjamina  and A. heterophyllus  were determined to be the most potential. Further characterizations suggested that F. benjamina  and A. heterophyllus  protease were optimum at pH 7.0 also at 50°C and 40°C, respectively. At their optimum conditions, both proteases  exhibited a lower MCA/PA ratio than that of the rennet. This study contributed to scientific knowledge development by becoming the first to characterize the optimum conditions of F. benjamina and A. heterophyllus’ proteases, investigate their MCA/PA ratio, and compare their activity against commercial rennet. The examination of their potentials as rennet alternatives could benefit small cheese industries and the communities.

Kristiani EBE, Kasmiyati S, Herawati MM. 2021. The cytotoxic and apoptotic effects of wild and polyploidy genotype of Artemisia cina extracts on the WiDr colon and HTB-183 lung cancer cell lines. Biodiversitas 22: 2844-2852. Artemisia belongs to the Asteraceae family, usually used for traditional treatments of various diseases in China. Artemisia cina Berg ex Poljakov, which lots found in Indonesia, has not been studied much like a cancer drug. The research aims were (i) to compare the bioactive content of hexane and ethyl acetate extract of wild (TWN and KJT) and polyploidy type (J and M) of A. cina and its cytotoxicity on WiDr and HTB-183 cancer cells, (ii) to evaluate the cytotoxicity mechanism of the most substantial extract of it. All of the extracts were prepared by maceration methods using hexane and ethyl acetate separately. The research method used was quantitative with experimental design. The determination of quercetin, kaempferol, and artemisinin used HPLC. The cytotoxicity of the extract determined using the MTT method. The assay of the specific protein related to apoptosis using ICC assay. The content of three bioactive compounds on ethyl acetate extracts higher than hexane extract, in which the extract of J and KJT genotypes had higher levels than M and TWN genotypes. The IC50 value (µg/mL) of extracts against cancer cell lines tested was about 400-700 hexane extracts and 200-500 ethyl acetate extracts. The BCl-2 expressions of both cancer cell lines decreased by treating TWN-EA and M-EA, while the P53 expressions increased. The TWN-EA induced apoptosis of HTB-183 cell lines through caspase-8 and caspase-9 pathways, but M-EA induced caspase-9 pathway only. The TWN and M genotypes were potential to use as anticancer agents on colon and lung cancer.

The effects of mioinositol and yeast extract were studied to assess their influence on growth of plantlets of Artemisia vulgaris by shoot culture. The plants regeneration of A. vulgaris were established by removing the nodes of stem and growing in MS multiplication medium with 1 ppm kinetin and 1 ppm NAA for 4 weeks. Shoots were induced for roots on MS treatment medium supplemented with mioinositol and yeast extract, added with 2 ppm IBA. Combination of four levels mioinositol concentration (mg/l): 100, 200, 300, and 400, and four levels of yeast extract concentration (mg/l): 0, 200, 300, and 400 were simultaneously added. Plantlets (2 weeks) were sub cultured on semi liquid MS medium. Plantlets were harvested on 6 weeks old. Measured parameters were fresh weight of plantlets. Data were analyzed using ANOVA followed by HSD test (p=95%). The results showed that the treatment of mioinositol and yeast extract were not significantly influenced on fresh weight of plantlets. Yeast extract was not influenced the growth of plantlets. The growth and morphogenesis of plantlets A. vulgaris were induced in treatment 100 ppm mioinositol, and addition mioinositol were higher than 100 ppm not significantly influenced the growth of plantlets.

Kasmiyati S, Kristiani EBE, Herawati MM, Rondonuwu FS. 2021. Exploring anticancer activity of wild and polyploid mutant of Artemisia cina. Biodiversitas 22: 1227-1234. The research aims were to explore the anticancer compounds in wild type (W) and polyploid mutant (P) of Artemisia cina Berg ex Poljakov using NIR and compare their cytotoxicity and selectivity on WiDR colon cancer and HTB183 lung cancer cell lines and Vero normal cell line. The W was obtained from B2P2TOOT Tawangmangu, Indonesia while P was by inducing A. cina shoot culture with 100 mg/l colchicine for 48 hours. They were extracted with hexane using the soxhletation method for 6 hours. The anticancer compounds were detected using NIR. The cytotoxic activity was determined using MTT assay, with Doxorubicin (D) as a control. The calculation of IC50 value used SPSS16 with a Probit analysis. The P contained the determined four compounds while the W contained no rutin. The IC50 values of W, P, and D were 295.5, 84.1, and 0.5 µg/mL on WiDr, 322.4, 128.6, and 39.9 µg/mL on HTB 183, whereas on Vero were 104, 315.6, and 295.5 µg/mL respectively. The selectivity indexes of W, P, and D were 34, 4, and 91 on WiDr, while HTB 183 was 31, 3, and 7, respectively. The P contained artemisinin, quercetin, kaemferol, and rutin, while the W contained no rutin. The cytotoxicity of both plants was less than doxorubicin. Both plants were selective on WiDR and HTB 183.

One of heavy metal pollutants in the soil that can be absorbed by the sorghum is chromium (Cr). The study was conducted to determine the growth response of Cr3+ stress of sorghum cultivars. Two chemical compounds Cr3+ and 3 level concentrations were exposed to sorghum cultivars. The research was conducted in two separate experiments i.e. during seed germination and early seedling development stages. The parameters measured were radicle/root length, seedling length, fresh weight, dry weight, and stress tolerance index (STI) value. The results showed that Cr3+ either in form of CrCl3 or KCr(SO4)2 significantly reduced the seedling growth of sorghum cultivars. The growth responses of sorghum cultivars toward Cr3+ stress showed differences both on stage of the germination and early seedling. Based on the average of STI value, four sorghum cultivars (Badik, Keris, Keris M3 and Numbu) were classified as very strong tolerant, 4 cultivars (Hegari, Mandau, Sangkur and Gambela) were categorized as moderate tolerant, two cultivars (UPCA and Selayer) were weak tolerant, and 2 cultivars (Kawali and Batari) were sensitive ones, under stress condition of Cr3+.The results of this study are expected to provide the scientific basis of the physiological and tolerance responses of sorghum cultivars toward Cr3+ stress condition.How to CiteKasmiyati, S., Santosa, S., Priyambada, I., Dewi, K., Sucahyo, S., & Sandradewi, R. (2016). Growth Response of Sorghum bicolor (L.) Moench. Cultivars to Trivalent Chromium Stress. Biosaintifika: Journal of Biology & Biology Education, 8(1), 71-84.

INTISARI Dewasa ini kegiatan pertanian tidak terlepas dari penggunaan herbisida kimia. Beberapa herbisida komersial menggunakan bahan aktif berupa metil metsulfuron (C14H15N5O6S) yang pada dasarnya bekerja mengubah pengaruh bahan kimia di dalam jaringan gulma yang dapat mematikan jaringan atau merusak sistem fisiologis yang dibutuhkan untuk kelangsungan hidup gulma. Karena pengaruh tersebut maka muncul pertanyaan apakah senyawa ini juga dapat mempengaruhi organisme non target seperti alga perifiton. Perifiton merupakan sekelompok organisme mikroskopis yang tumbuh menempel pada permukaan benda atau tumbuhan air, tidak menembus substrat, diam atau bergerak di permukaan substrat tersebut. Salah satu penyusun utama komunitas perifiton adalah alga. Alga perifiton di perairan memiliki peran yang besar dalam menentukan produktivitas primer dan sebagai bioindikator kualitas perairan. Beberapa penelitian yang telah dilakukan sebelumnnya menunjukkan jika beberapa bahan aktif pada konsentrasi tertentu berpengaruh signifikan terhadap produktivitas primer alga perifiton dan dapat menghambat pertumbuhan spesies alga tertentu. Penelitian ini dilakukan untuk mengetahui pengaruh herbisida berbahan aktif metil metsulfuron terhadap struktur komunitas alga perifiton. Parameter yang diukur antara lain kandungan klorofil a, nilai kepadatan, nilai kekayaan, indeks keanekaragaman, indeks keseragaman, dan indeks dominansi serta identifikasi spesies alga penyusun perifiton. Penelitian dilakukan dalam skala laboratorium menggunakan sampel yang diambil dari Danau Rawa Pening, Kab. Semarang. Pengamatan dilakukan selama 21 hari dengan pemaparan herbisida dengan konsentrasi 0; 0,05; 0,1; 0,2; dan 0,4 mg/l. Hasil penelitian menunjukkan bahwa konsentrasi metil metsulfuron dan lama pemaparan berpengaruh signifikan terhadap kandungan klorofil a (p0,05). Kata kunci: Alga Perifiton, Metil Metsulfuron, Struktur Komunitas

Artemisinin is a sesquiterpene lactone found in Artemisia cina having many medicinal properties. Artemisinin produced and stored in Glandular trichomes The purpose of this study was to determine the effect of shade 0%, 50% and 75% on the density and size of glandular trichomes and the content of artemisinin in A.cina tetraploid plants. The shade treatment significantly affected the density and size of glandular trichomes and the content of artemisinin in Artemisia cina tetraploid. 50% shade treatment showed the highest density of Glandular trichome, the width of glandular trichomes, and Artemisinin content. The results of the correlation analysis show that there is a very significant relationship between the density of glandular trichomes and the content of artemisinin.

Increased production of reactive oxygen species or ROS is one of the common responses to a wide range of biotic and abiotic stresses. Increased production of ROS is outstripping endogenous antioxidant defense systems has been referred to as oxidative stress. Heavy metals are known to initiate ROS generation which is implicated as a oxidative stress. Cr is a toxic heavy metal that can generate ROS like H2O2 and O2 - which cause oxidative stress. In this study, chromium toxicity was studied to detect the oxidative stress on Sonchus oleraceus weed plants by the detection of superoxide anion and H2O2. Superoxide anion was detected by staining techniques with nitroblue tetrazolium (NBT) and hydrogen peroxide by Diaminobenzidine tetrahydrochloride (DAB) staining. Results indicated that the plants were grown in sand media generate the highest (0.89 A/g FW and 3.23 mol/g FW) than in soil media (0.23 A/g FW and 2.11 mol/g FW) superoxide anion (*O- 2) and H2O2 and soil containing textile sludge (0.18 A/g FW and 2.66 mol/g FW), respectively. At application of 10 mg Cr6+/L and 250 mg Cr3+/L, the production of *O- 2 and H2O2 in leaves of sonchus plants were significantly increased compared with the control plants. The highest production of H2O2 and *O- 2 were showed in the leaves of sonchus plants grown in sand media with Cr6+ application. In this study, either Cr3+ or Cr6+ caused oxidative stress in Sonchus oleraceus weed plants. The result also showed that sonchus plants esposed to toxic Cr can suffer from oxidative stress leading to reduction of its fresh and dry plants biomass. NBT and DAB in an appropriate probe and significant value for monitoring the formation of *O- 2 and H2O2 in plants.

The objectives of this reserch were to determine the antibacterial activity of hexane-petroleum ether (1 : 1 v/v) extract of Artemisia cinaBerg. ex Poljakov on Escherichia coli and Staphylococcus aureus and its compounds. The design of the research was using completely Randomized Design in five concentration of extract that were 0, 50, 75, 100, 150, dan 200 mg/ml with four repliceted. Ethanol 60% was used as negative control and tetracycline as positive control. There were three steps of research. First step was extracted the plant using soxhlet method with hexane-petroleum ether (1:1 v/v). Second step was determined the antibacterial activity of hexane-petroleum ether (1:1 v/v) extract in various concentration of that extracts on E. Coli and S. aureus using agar diffusion method. Analysis of Variance (ANOVA) and was used to determined the significan different of diameter of inhibition between the treatments. Thirdsteps was phytochemical analysis of extract. The highest antibactrial activity on E. Coli was at 100 mg/ml whereas on S. Aureus at 150 mg/ml. That extract was contained flavonoid, alkaloid, essential oils, saponin, sterol, tritepene, hydrolized tannin, and coumarin.

Moringa oleifera and Muntingia calabura leaves have been reported to have an antibacterial activity that could inhibit the growth of gram positive and negative bacteria. However, the antibacterial activity of mixed extracts of M. oleifera seeds and M. calabura leaves has not been widely reported. The purpose of this study was to test antibacterial activity of the mixed extract of M. oleifera seeds and M. calabura leaves on the growth of Pseudomonas aeruginosa and Bacillus subtilis . The experiment was conducted by agar disc diffusion method using three groups of extract treatments i.e. M.oleifera seeds extract, M.calabura leaves extract, and mixed extracts of M. oleifera seeds and M. calabura leaves with a ratio of 1: 1 (v / v). The extraction of M. oleifera seeds and M. calabura leaves was conducted by soxhlation method and using ethanol as solvent. The three groups of extract treatments with a concentration of 400 ppm, 800 ppm, 1200 ppm, and 1600 ppm were tested on P. aeruginosa . The antibacterial activity test of M. oleifera seed extract against B. subtilis carried out at the level of concentrations i.e. 150 ppm, 300 ppm, 450 ppm, 600 ppm, and 750 ppm, meanwhile, M. calabura leaves extract was done at concentration 1500 ppm, 3000 ppm, 4500 ppm, 6000 ppm, and 7500 ppm. The result showed that the three groups of extract treatments possess antibacterial activity against P. aeruginosa . The mixed extracts of M. oleifera seeds and M. calabura leaves with a ratio of 1: 1 (v / v) at level concentration of 400 ppm and 800 ppm were tested against P. aeruginosa significantly increased, and at concentrations of 1200 ppm and 1600 ppm significantly decreased the inhibition diameter of bacterial growth than the other extracts treatments. The antibacterial test results of M.oleifera seeds extract and M.calabura leaves extract against B. subtilis shows that increased concentrations of the extract significantly increase the inhibition diameter of bacterial growth especially at high concentrations ( 600 ppm and 750 ppm) on M. oleifera seeds extract, as well as 6000 ppm and 7500 ppm in M. calabura leaves extract.

In contrast to other toxic trace metals, Cr has received little attention. Since valence level of chromium determines its toxicity, chromium is categorized as unique heavy metal. Chromium hexavalent (Cr 6+ ) has the biggest toxicity among other valence levels. Seed germination and seedling growth are sensitive to heavy metal stresses. This research aimed to find out the responses of seed germination and seedling growth of 12 sorghum varieties toward Cr 6+ . Seed germination and seedling growth experiment was done on 12 sorghum varieties (Badik, UPCA-1, Keris, Keris M3, hegari Genjah, Gambela, Selayer, Sangkur, Mandau, Batari, Kawali, dan Numbu), planted in petridishes with Cr 6+ treatment in form of chromate (K 2 CrO 4 ) and dichromate (K 2 Cr 2 O 7 ) compounds with 0, 50 and 500 mg of Cr/l concentration for a week. The parameters measured were the number of seeds germinate each day; the length of radicle and plumule, and fresh and dry weight at the end of experiment. The results showed that higher concentration of Cr 6+ both in form of dichromate and chromate, significantly decreased the length of radicle and plumule, fresh and dry weight, and SVI (seedling vigor index) value. However, index germination (GI) value and percentage of germination of the 12 varieties sorgum significantly increased in the treatment of 50 mg Cr/l Cr 6+ in form of dichromate and chromate. The treatment of dichromate compound showed bigger effect than chromate toward variables of seed germination and seedling growth of sorghum. It was noticed that 12 sorghum varieties possessed an integrated complex of adaptation to cope with the range of form of compound and concentration of Cr 6+ . Based on the responses of seed germination and seedling growth, Kawali, Hegari, Keris, Keris M3, Mandau, and Selayer varieties was more susceptible toward Cr 6+ toxicity, and Sangkur, Selayer, Batari, and Numbu was more tolerant than other varieties. Keywords : chromate, dichromate, Sorghum bicolor, seedling, hexavalent chromium