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Mouse bone marrow stromal stem/progenitor cells (BMSCs, also known as bone marrow-derived mesenchymal stem cells) and Hematopoietic Stem and Progenitor Cells (HSPCs) with differential proliferative potentials were investigated for identifying epigenetic signals that can modulate their growth. In the present study, immunodepletion of granulo-monocytic (CD11b) and erythroid (Ter119) population yielded CD11b−/Ter119− cells, capable of differentiating into chondrogenic, osteogenic and adipogenic cells. Enrichment of the CD11b+ population by positive selection of multipotent stem/progenitor marker (CD133) yielded CD11b+/CD133+ cells, efficiently differentiated into hematopoietic lineages. Molecular characterization revealed the expression of BMSC and HSPC markers in CD11b−/Ter119− and CD11b+/CD133+ sorted populations, respectively. Cell expansion studies depicted a higher growth rate and percentage of proliferating cells in G2/M phase of cell cycle in BMSCs (13.9 ± 2.9%) as compared with HSPCs (5.8 ± 0.8%). Analysis of the HDACs gene expression revealed a differential expression pattern in BMSCs and HSPCs that modulates the cell cycle genes. Trichostatin A (TSA)-mediated HDAC inhibition led to an increased level of AcH3 and AcH4 along with cyclins B1 and D2. Chromatin immunoprecipitation revealed alleviation of HDAC2 and HDAC3 binding by TSA on cyclins B1 and D2 promoter, thereby enhancing cell proliferation. This study identifies epigenetic modulation on the proliferative potential of BMSCs and HSPCs for stem cell transplantation therapies.

An OTP, or One-Time Password, is an extra security mechanism for online transactions that involves two-step authentication. For most financial transactions, this damn time OTP has become a very popular option. Because of its increasing popularity, it is becoming more accessible to fraudsters who try to steal your OTP in order to compromise your account or perform illegal financial transactions. The Proposed system provides a solution and works well as a multiple authentication security mechanism and has a lot of configuration options built in.This protects against a range of social, personalized, and opportunistic attacks. Outsiders are unable to gain access to the cloud infrastructure unless the customer has correctly authenticated by the cloud service provider. The cloud offers four priority levels of security that would provide a strong barrier against unauthorized access to the user account. The Created Cloud Id, or UID, will play an important role in gaining access to the user's cloud account and to the applications utilized by the User. In terms of experience, professionals, resources, and firmware, the proposed framework allows businesses to spend very little on OTP - based TFA transfer. It also allows the user to manage multiple accounts from one location, and via unlikable profiles. It is thought that outsourcing OTP service to the cloud will make it easier for many cloud service providers to enforce bundle OTP because it does not demand extra investment. [ABSTRACT FROM AUTHOR]

The key for understanding the behavior and vitality of an insect is to study the effect of food, temperature and changing factors responsible for insect development. The effect on prey intake on the development of predator varies distinctly with the prey consumed. The study was conducted to understand the impact of different hosts on development of anthocorid predator, Blaptostethus pallescens under laboratory conditions. The studywas carried out on the sucking pests like thrips, red spider mites, nymphs of papaya mealybug and eggs of rice moth. The results showed that incubation period lasted for 5.57±0.37 days in thrips followed by papaya mealy bug crawlers (5.14±0.26 days) which was the longest duration among the hosts evaluated. Papaya mealybug nymphs had longest average nymphal period (23 days) whereas shortest was observed in rice moth eggs (17 days). Adult longevity was highest in rice moth eggs (47 days)compared over other prey. The total number of instars remained constant among all the host i.e., 5 nymphal instars during the complete development of the predator. The experimental results indicated that alternate hosts containing thrips and mites can be incorporated in field for maintaining predator population in absence of primary hosts. [ABSTRACT FROM AUTHOR]

Introduction: Lichenoid tissue reaction or interface dermatitis embraces several clinical conditions, the prototype of which is lichen planus and its variants. Others include drug induced lichenoid dermatitis, special forms of lichenoid dermatitis, lichenoid dermatitis in lupus erythematosus, and other disorders all of which have salient clinical and histological features. The aim of this study was to correlate the clinical features with histopathological findings in all clinically suspected cases of lichen planus and assess the clinical diagnostic accuracy. Materials and methods: This study had been carried out over a period of 2 years from July 2015 to June 2017 in the Department of Pathology, Gandhi Hospital. Histopathological sections of skin biopsies from 85 patients were studied after routine H and E staining and morphological changes were noted. The patients' clinical findings were then correlated with the histological changes. The results were then analysed. Results: Total numbers of biopsies inclusive of LP and LP like lesions were 85. Among them, the number of patients having LP was 50. The age ranged from 18 to 65 years (mean 37.1 years). The mean duration of the lesion was 12.02 months (approx 1 year). Males were affected more than females. Out of the 85 cases, 50 were Lichen planus and 35 were lichenoid eruptions. Of the Lichen planus, 31 (61.6%) cases were of classical lichen planus, 6 (13.3%) cases were of hypertrophic lichen planus, 4 (8.3%) cases were of lichen planus pigmentosus, 4 (6.6%) cases were of actinic lichen planus, 3 (5%) cases were of eruptive lichen planus, 1 (1.6%) case each of atrophic lichen planus, annular lichen planus. Conclusion: In the present study, maximum number of cases occurred in age group of 18-28 years. Though pruritis is a common symptom, it is nonspecific. Therefore, histopathology remains the most important diagnostic tool for proper evaluation of Lichenoid eruptions/ Lichen planus. [ABSTRACT FROM AUTHOR]

The cardiovascular dis-orders are increasing day by day. Coronary artery disease (CAD) is currently a leading cause of death worldwide. Drug-eluting stents (DESs) have been dominant for the treatment of CAD in the interventional cardiology world owing to their efficacy in significantly reducing restenosis as compare to metallic stents. However, late stage stent thrombosis has become a major concern. Stent platform, drug delivery vehicle and types of drugs are three parts of DES and each part affects the performance of the DES. This review gives real answer about safety control of restenosis and thrombosis in CAD by using drug eluting stent. [ABSTRACT FROM AUTHOR]

Polymer electrolyte membrane fuel cells (PEMFCs) suffer from severe performance degradation when operating under harsh conditions such as fuel starvation, shut-down/start-up, and open circuit voltage. A fundamental solution to these technical issues requires an integrated approach rather than condition-specific solutions. In this study, an anode catalyst based on Pt nanoparticles encapsulated in a multifunctional carbon layer (MCL), acting as a molecular sieve layer and protective layer is designed. The MCL enabled selective hydrogen oxidation reaction on the surface of the Pt nanoparticles while preventing their dissolution and agglomeration. Thus, the structural deterioration of a membrane electrode assembly can be effectively suppressed under various harsh operating conditions. The results demonstrated that redesigning the anode catalyst structure can serve as a promising strategy to maximize the service life of the current PEMFC system., (© 2024 The Authors. Advanced Science published by Wiley‐VCH GmbH.)

Intriguing cationic defects with hollow nano-/microstructures are a critical challenge but a potential strategy to discover electrochemical energy conversion and storage devices with improved electrochemical performances. Herein, we successfully produced a highly porous, and large surface area of self-templated CuCo 2 O 4 hollow spheres (CCOHSs) with cationic defects via a solvothermal route. We hypothesized that the inside-out Ostwald ripening mechanism of the template-free strategy was the framework for forming the CCOHSs. Cationic defects (Cu) within the CCOHSs were identified by employing various analytical techniques, including energy-dispersive X-ray spectroscopy analysis of both scanning and transmission electron microscopy, X-ray photon spectroscopy, and inductively coupled plasma-atomic emission spectroscopy. The resulting CCOHSs had significant properties, such as a high specific surface area of 98.32 m 2 g -1 , rich porosity, and battery-type electrode behavior in supercapacitor applications. Notably, the CCOHSs demonstrated an outstanding specific capacity of 1003.7 C g -1 at 1 A g -1 , with excellent structural integrity and cycle stability. Moreover, the fabricated asymmetric CCOHS//activated carbon device exhibited a high energy density of 65.2 Wh kg -1 at a power density of 777.8 W kg -1 .

The development of non-Pt or carbon-based catalysts for anion exchange membrane fuel cells (AEMFCs) requires identification of the active sites of the catalyst. Since not only metals but also carbon materials exhibit oxygen reduction reaction (ORR) activity in alkaline conditions, the contribution of carbon-based materials to ORR performance should also be thoroughly analyzed. However, the conventional CN - poisoning experiments, which are mainly used to explain the main active site of M-N-C catalysts, are limited to only qualitative discussions, having the potential to make fundamental errors. Here, we report a modified electrochemical analysis to quantitatively investigate the contribution of the metal and carbon active sites to ORR currents at a fixed potential by sequentially performing chronoamperometry with two reaction inhibitors, CN - and benzyl trimethylammonium (BTMA + ). As a result, we discover how to quantify the individual contributions of two active sites (Pt nanoparticles and carbon support) of carbon-supported Pt (Pt/C) nanoparticles as a model catalyst. This study is expected to provide important clues for the active site analysis of carbon-supported non-Pt catalysts, such as M-N-C catalysts composed of heterogeneous elements.

Graphene is an intriguing two-dimensional honeycomb-like carbon material with a unique basal plane structure, charge carrier mobility, thermal conductivity, wide electrochemical spectrum, and unusual physicochemical properties. Therefore, it has attracted considerable scientific interest in the field of nanoscience and bionanotechnology. The high specific surface area of graphene allows it to support high biomolecule loading for good detection sensitivity. As such, graphene, graphene oxide (GO), and reduced GO are excellent materials for the fabrication of new nanocomposites and electrochemical sensors. Graphene has been widely used as a chemical building block and/or scaffold with various materials to create highly sensitive and selective electrochemical sensing microdevices. Over the past decade, significant advancements have been made by utilizing graphene and graphene-based nanocomposites to design electrochemical sensors with enhanced analytical performance. This review focus on the synthetic strategies, as well as the structure-to-function studies of graphene, electrochemistry, novel multi nanocomposites combining graphene, limit of detection, stability, sensitivity, assay time. Finally, the review describes the challenges, strategies and outlook on the future development of graphene sensors technology that would be usable for the internet of things are also highlighted., (Copyright © 2022 Elsevier B.V. All rights reserved.)

Herein, we describe a high-performance electrochemical sensor for the detection of regorafenib (REG) using bimetallic Pd-Ru nanoparticles anchored on pomegranate peel extract (PPE) derived reduced graphene oxide (Pd-Ru/rGO). PPE was employed to neutralize the extremely acidic graphene then cast-off along with the metal precursor for the duration of the chemical reduction to accomplish well dispersed Pd-Ru nanoparticles. Bimetallic Pd-Ru/rGO nanocomposites were synthesized using a facile chemical reduction method. Under optimal conditions, based on the differential pulse voltammetric studies it has been confirmed that the fabricated sensors has good electrocatalytic activity toward the detection of REG, spanning over the linear dynamic range of 0.5-300 nM. Moreover, the sensor exhibited a low limit of detection of 1.6 nM and a limit of quantification of 4.8 nM. The electrochemical sensor unveiled admirable selectivity and sensitivity, reproducibility, and repeatability. The fabricated sensor was suitable for real sample analysis (pharmaceutical tablet, human blood plasm, wastewater) with satisfactory recovery. The strategy presented herein can be employed in the development of electrochemical sensors for other target analytes., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Use of Pt-based electro-catalysts for the oxygen reduction reaction (ORR) is a major hindrance in large-scale application of proton exchange membrane fuel cells (PEMFCs). Hence, new, cost-effective and high performance electro-catalysts are required for the commercial success of PEMFCs. In this work, a Pt-free magnesium oxide (MgO) decorated multi-layered reduced graphene oxide (MLGO) composite is tested as an electro-catalyst for the ORR. The ORR activity of MgO/MLGO in terms of diffusion-controlled current density is found to be superior (6.63 mA per cm2-geo) than that of in-house prepared Pt/rGO (5.96 mA per cm2-geo) and commercial Pt/C (5.02 mA per cm2-geo). The applicability of less expensive MgO/MLGO not only provides a new electro-catalyst but also provides a new direction in exploring metal oxide-based electro-catalysts for the ORR.