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32 results on '"Spyratou, Ellas"'

1. Advanced Biophotonics techniques for cell and molecules manipulation associated with cancer and autoimmune diseases: the role of optical tweezers

Author

Spyratou, Ellas

Subjects
Physics - Medical Physics
Abstract

Biophotonic techniques are growing in rapid rhythms enabling the monitoring of subcellular structures and non-invasive theranostic interventions in cancer and autoimmune diseases. The integration of Biophotonics with nanotechnology and biosensors brings a revolution in the micro- and nano-world with new optical tools. Among them, optical tweezers revive as a potential tool for tracking cells behavior and probing interactions forces between cells, cells-biomolecules and cells-nanoparticles. In this review we aim to exhibit the state-of the art advances of the Biophotonics in the diagnostic and therapeutic field, and the role of optical tweezers., Comment: 14 pages, 5 Figures

Published
2021

2. Nanoparticle-Mediated Radiotherapy: Unraveling Dose Enhancement and Apoptotic Responses in Cancer and Normal Cell Lines

Author

Kouri, Maria Anthi, primary, Spyratou, Ellas, additional, Kalkou, Maria-Eleni, additional, Patatoukas, Georgios, additional, Angelopoulou, Evangelia, additional, Tremi, Ioanna, additional, Havaki, Sophia, additional, Gorgoulis, Vassilis G., additional, Kouloulias, Vassilis, additional, Platoni, Kalliopi, additional, and Efstathopoulos, Efstathios P., additional

Published
2023
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3. Extended Analysis of Raman Spectra Using Artificial Intelligence Techniques for Colorectal Abnormality Classification

Author

Kalatzis, Dimitris, primary, Spyratou, Ellas, additional, Karnachoriti, Maria, additional, Kouri, Maria Anthi, additional, Stathopoulos, Ioannis, additional, Danias, Nikolaos, additional, Arkadopoulos, Nikolaos, additional, Orfanoudakis, Spyros, additional, Seimenis, Ioannis, additional, Kontos, Athanassios G., additional, and Efstathopoulos, Efstathios P., additional

Published
2023
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4. Shedding Light on Colorectal Cancer: An in Vivo Raman Spectroscopy Approach Combined with Deep Learning Analysis

Author

Kouri, Maria Anthi, primary, Karnachoriti, Maria, additional, Spyratou, Ellas, additional, Orfanoudakis, Spyros, additional, Kalatzis, Dimitris, additional, Kontos, Athanassios G., additional, Seimenis, Ioannis, additional, Efstathopoulos, Efstathios P., additional, Tsaroucha, Alexandra, additional, and Lambropoulou, Maria, additional

Published
2023
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6. Advanced Raman Spectroscopy Based on Transfer Learning by Using a Convolutional Neural Network for Personalized Colorectal Cancer Diagnosis

Author

Kalatzis, Dimitris, primary, Spyratou, Ellas, additional, Karnachoriti, Maria, additional, Kouri, Maria Anthi, additional, Orfanoudakis, Spyros, additional, Koufopoulos, Nektarios, additional, Pouliakis, Abraham, additional, Danias, Nikolaos, additional, Seimenis, Ioannis, additional, Kontos, Athanassios G., additional, and Efstathopoulos, Efstathios P., additional

Published
2023
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12. FDXR Gene Expression after in Vivo Radiation Exposure of Pediatric Patients Undergoing Interventional Cardiology Procedures

Author

Spyratou, Ellas, primary, Ploussi, Agapi, primary, Alafogiannis, Panagiotis, primary, Katifelis, Hector, primary, Apostolopoulou, Sotiria, primary, Bagenakis, Georgios, primary, Rammos, Spyridon, primary, Papagiannis, Ioannis, primary, Gazouli, Maria, primary, Seimenis, Ioannis, primary, Georgakilas, Alexandros G., primary, and Efstathopoulos, Efstathios P., primary

Published
2022
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13. Probing the effects of ionizing radiation on young's modulus of human erythrocytes cytoskeleton using atomic force microscopy

Author

Spyratou, Ellas, Dilvoi, Maria, Patatoukas, George, Platoni, Kalliopi, Makropoulou, Mersini, and Efstathopoulos, Efstathios

Subjects
Atomic force microscopy, Ionizing radiation, Anticoagulants, Microscopy, Radiation (Physics), Company legal issue, Health, International Atomic Energy Agency -- Investigations
Abstract

Byline: Ellas. Spyratou, Maria. Dilvoi, George. Patatoukas, Kalliopi. Platoni, Mersini. Makropoulou, Efstathios. Efstathopoulos Purpose/Aim: In this work, we examined the possible effects of ionizing radiation (IR) on biomechanical properties of [...]

Published
2019

14. Raman Spectroscopy: A Personalized Decision-Making Tool on Clinicians’ Hands for In Situ Cancer Diagnosis and Surgery Guidance

Author

Kouri, Maria Anthi, primary, Spyratou, Ellas, additional, Karnachoriti, Maria, additional, Kalatzis, Dimitris, additional, Danias, Nikolaos, additional, Arkadopoulos, Nikolaos, additional, Seimenis, Ioannis, additional, Raptis, Yannis S., additional, Kontos, Athanassios G., additional, and Efstathopoulos, Efstathios P., additional

Published
2022
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16. Requirements for Designing an Effective Metallic Nanoparticle (NP)-Boosted Radiation Therapy (RT)

Author

Tremi, Ioanna Spyratou, Ellas Souli, Maria Efstathopoulos, Efstathios P. Makropoulou, Mersini Georgakilas, Alexandros G. and Sihver, Lembit

Abstract

Simple Summary Recent advances in nanotechnology gave rise to trials with various types of metallic nanoparticles (NPs) to enhance the radiosensitization of cancer cells while reducing or maintaining the normal tissue complication probability during radiation therapy. This work reviews the physical and chemical mechanisms leading to the enhancement of ionizing radiation’s detrimental effects on cells and tissues, as well as the plethora of experimental procedures to study these effects of the so-called “NPs’ radiosensitization”. The paper presents the need to a better understanding of all the phases of actions before applying metallic-based NPs in clinical practice to improve the effect of IR therapy. More physical and biological experiments especially in vivo must be performed and simulation Monte Carlo or mathematical codes based on more accurate models for all phases must be developed. Many different tumor-targeted strategies are under development worldwide to limit the side effects and improve the effectiveness of cancer therapies. One promising method is to enhance the radiosensitization of the cancer cells while reducing or maintaining the normal tissue complication probability during radiation therapy using metallic nanoparticles (NPs). Radiotherapy with MV photons is more commonly available and applied in cancer clinics than high LET particle radiotherapy, so the addition of high-Z NPs has the potential to further increase the efficacy of photon radiotherapy in terms of NP radiosensitization. Generally, when using X-rays, mainly the inner electron shells are ionized, which creates cascades of both low and high energy Auger electrons. When using high LET particles, mainly the outer shells are ionized, which give electrons with lower energies than when using X-rays. The amount of the produced low energy electrons is higher when exposing NPs to heavy charged particles than when exposing them to X-rays. Since ions traverse the material along tracks, and therefore give rise to a much more inhomogeneous dose distributions than X-rays, there might be a need to introduce a higher number of NPs when using ions compared to when using X-rays to create enough primary and secondary electrons to get the desired dose escalations. This raises the questions of toxicity. This paper provides a review of the fundamental processes controlling the outcome of metallic NP-boosted photon beam and ion beam radiation therapy and presents some experimental procedures to study the biological effects of NPs’ radiosensitization. The overview shows the need for more systematic studies of the behavior of NPs when exposed to different kinds of ionizing radiation before applying metallic-based NPs in clinical practice to improve the effect of IR therapy.

Published
2021

20. High predictive values of RBC membrane-based diagnostics by biophotonics in an integrated approach for Autism Spectrum Disorders

Author

Giacometti, Giorgia, primary, Ferreri, Carla, additional, Sansone, Anna, additional, Chatgilialoglu, Chryssostomos, additional, Marzetti, Carla, additional, Spyratou, Ellas, additional, Georgakilas, Alexandros G., additional, Marini, Marina, additional, Abruzzo, Provvidenza M., additional, Bolotta, Alessandra, additional, Ghezzo, Alessandro, additional, Minguzzi, Renato, additional, Posar, Annio, additional, and Visconti, Paola, additional

Published
2017
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21. Characterization of new drug delivery nanosystems using atomic force microscopy

Author

Spyratou, Ellas Mourelatou, Elena A. Demetzos, C. and Makropoulou, Mersini Serafetinides, A. A.

Abstract

Liposomes are the most attractive lipid vesicles for targeted drug delivery in nanomedicine, behaving also as cell models in biophotonics research. The characterization of the micro-mechanical properties of drug carriers is an important issue and many analytical techniques are employed, as, for example, optical tweezers and atomic force microscopy. In this work, polyol hyperbranched polymers (HBPs) have been employed along with liposomes for the preparation of new chimeric advanced drug delivery nanosystems (Chi-aDDnSs). Aliphatic polyester HBPs with three different pseudogenerations G2, G3 and G4 with 16, 32, and 64 peripheral hydroxyl groups, respectively, have been incorporated in liposomal formulation. The atomic force microscopy (AFM) technique was used for the comparative study of the morphology and the mechanical properties of Chi-aDDnSs and conventional DDnS. The effects of both the HBPs architecture and the polyesters pseudogeneration number in the stability and the stiffness of chi-aDDnSs were examined. From the force-distance curves of AFM spectroscopy, the Young’s modulus was calculated.

Published
2015

22. Structure–properties investigations in hydrophilic nanocomposites based on polyurethane/poly(2–hydroxyethyl methacrylate) semi‐interpenetrating polymer networks and nanofiller densil for biomedical application

Author

Klonos, Panagiotis, primary, Chatzidogiannaki, Vasileia, additional, Roumpos, Konstantinos, additional, Spyratou, Ellas, additional, Georgiopoulos, Panayiotis, additional, Kontou, Evagelia, additional, Pissis, Polycarpos, additional, Gomza, Yuriy, additional, Nesin, Stanislav, additional, Bondaruk, Oksana, additional, and Karabanova, Lyudmyla, additional

Published
2015
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26. Structure-properties investigations in hydrophilic nanocomposites based on polyurethane/poly(2-hydroxyethyl methacrylate) semi-interpenetrating polymer networks and nanofiller densil for biomedical application.

Author

Klonos, Panagiotis, Chatzidogiannaki, Vasileia, Roumpos, Konstantinos, Spyratou, Ellas, Georgiopoulos, Panayiotis, Kontou, Evagelia, Pissis, Polycarpos, Gomza, Yuriy, Nesin, Stanislav, Bondaruk, Oksana, and Karabanova, Lyudmyla

Subjects
POLYMERIC nanocomposites, POLYURETHANES, HYDROPHILIC compounds, NANOPARTICLE synthesis, DIFFERENTIAL scanning calorimetry
Abstract

Nanocomposites based on sequential semi-interpenetrating polymer networks (semi-IPNs) of crosslinked polyurethane and linear poly(2-hydroxyethyl methacrylate) filled with 1-15 wt % of nanofiller densil were prepared and investigated. Nanofiller densil used in an attempt to control the microphase separation of the polymer matrix by polymer-filler interactions. The morphology (SAXS, AFM), mechanical properties (stress-strain), thermal transitions (DSC) and polymer dynamics (DRS, TSDC) of the nanocomposites were investigated. Special attention has been paid to the raising of the hydration properties and the dynamics of water molecules in the nanocomposites in the perspective of biomedical applications. Nanoparticles were found to aggregate partially for higher than 3 and 5 wt % filler loading in semi-IPNs with 17 and 37 wt % PHEMA, respectively. The results show that the good hydration properties of the semi-IPN matrix are preserved in the nanocomposites, which in combination with results of thermal and dielectric techniques revealed also the existence of polymer-polymer and polymer-filler interactions. These interactions results also in the improvement of physical and mechanical properties of the nanocomposites in compare with the neat matrix. The improvement of mechanical properties in combination with hydrophilicity and biocompatibility of nanocomposites are promising for use these materials for biomedical application namely as surgical films for wound treatment and as material for producing the medical devises. [ABSTRACT FROM AUTHOR]

Published
2016
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30. Measurements of liposome biomechanical properties by combining line optical tweezers and dielectrophoresis.

Author

Spyratou, Ellas, Cunaj, Efrosini, Tsigaridas, George, Mourelatou, Elena A., Demetzos, Costas, Serafetinides, Alexander A., and Makropoulou, Mersini

Subjects
*LIPOSOMES, *OPTICAL tweezers, *DIELECTROPHORESIS, *HUMAN physiology, *ELASTICITY
Abstract

Liposomes are well-known cell simulators and are currently studied as drug delivery systems, for a targeted delivery of higher drug concentrations, in specific cells. Novel biophotonic techniques for manipulation and characterization of liposomes have been developed; among which are optical tweezers. In our work, we demonstrate a novel use of line optical tweezers to manipulate and cause liposome deformations. Optical forces induce tension on liposomes, which are stretched along the line optical trap. The method of dielectrophoresis, combined with optical tweezers, was used to measure the exerted optical forces. As a consequence, in the case of reversible liposome deformations, the value of the shear and bending moduli of liposomes was calculated. We anticipate that the selective manipulation of liposomes will help us toward a better understanding of the cellular–liposome interactions. Studying the biomechanical properties of liposomes will provide an insight into the mechanical behavior of individual living cells, which have recently been implicated in many aspects of human physiology and patho-physiology. The biomechanical properties of cells (i.e. deformability, stiffness and elasticity) can be useful biomarkers for various disease processes and changes of the cell state. [ABSTRACT FROM AUTHOR]

Published
2015
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