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5 results on '"Spitsyna, Anna S."'

1. Stability of ZIF-8 Nanoparticles in Most Common Cell Culture Media

Author

Spitsyna, Anna S., primary, Poryvaev, Artem S., additional, Sannikova, Natalya E., additional, Yazikova, Anastasiya A., additional, Kirilyuk, Igor A., additional, Dobrynin, Sergey A., additional, Chinak, Olga A., additional, Fedin, Matvey V., additional, and Krumkacheva, Olesya A., additional

Published
2022
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2. Methanethiosulfonate Derivative of OX063 Trityl: A Promising and Efficient Reagent for Side‐Directed Spin Labeling of Proteins

Author

Tormyshev, Victor M., primary, Chubarov, Alexey S., additional, Krumkacheva, Olesya A., additional, Trukhin, Dmitry V., additional, Rogozhnikova, Olga Yu., additional, Spitsyna, Anna S., additional, Kuzhelev, Andrey A., additional, Koval, Vladimir V., additional, Fedin, Matvey V., additional, Godovikova, Tatyana S., additional, Bowman, Michael K., additional, and Bagryanskaya, Elena G., additional

Published
2020
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3. Protein modification by thiolactone homocysteine chemistry: a multifunctionalized human serum albumin theranostic

Author

Popova, Tatyana V., primary, Krumkacheva, Olesya A., additional, Burmakova, Anna S., additional, Spitsyna, Anna S., additional, Zakharova, Olga D., additional, Lisitskiy, Vladimir A., additional, Kirilyuk, Igor A., additional, Silnikov, Vladimir N., additional, Bowman, Michael K., additional, Bagryanskaya, Elena G., additional, and Godovikova, Tatyana S., additional

Published
2020
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4. Protein modification by thiolactone homocysteine chemistry: a multifunctionalized human serum albumin theranosticElectronic supplementary information (ESI) available: Spectroscopic materials and methods, additional spectra (UV-vis absorption, fluorescence, NMR), preparation of HSA modified by sulfo-Cy5 dye (HSA-Cy5), size exclusion chromatography (SEC), and in vitrocytotoxicity assay. See DOI: 10.1039/c9md00516a

Author

Popova, Tatyana V., Krumkacheva, Olesya A., Burmakova, Anna S., Spitsyna, Anna S., Zakharova, Olga D., Lisitskiy, Vladimir A., Kirilyuk, Igor A., Silnikov, Vladimir N., Bowman, Michael K., Bagryanskaya, Elena G., and Godovikova, Tatyana S.

Abstract

As the most abundant protein with a variety of physiological functions, albumin has been used extensively for the delivery of therapeutic molecules. Thiolactone chemistry provides a powerful tool to prepare spin-labeled albumin-based multimodal imaging probes and therapeutic agents. We report the synthesis of a tamoxifen homocysteine thiolactone derivative and its use in thiol-‘click’ chemistry to prepare multi-functionalized serum albumin. The released sulfhydryl group of the homocysteine functional handle was labeled with a nitroxide reagent to prepare a spin-labeled albumin–tamoxifen conjugate confirmed by MALDI-TOF-MS, EPR spectroscopy, UV-vis and fluorescent emission spectra. This is the basis for a novel multimodal tamoxifen–albumin theranostic with a significant (dose-dependent) inhibitory effect on the proliferation of malignant cells. The response of human glioblastoma multiforme T98G cells and breast cancer MCF-7 cells to tamoxifen and its albumin conjugates was different in tumor cells with different expression level of ERα in our experiments. These results provide further impetus to develop a serum protein for delivery of tamoxifen to cancer cells.

Published
2020
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5. Methanethiosulfonate Derivative of OX063 Trityl: A Promising and Efficient Reagent for Side-Directed Spin Labeling of Proteins.

Author

Tormyshev VM, Chubarov AS, Krumkacheva OA, Trukhin DV, Rogozhnikova OY, Spitsyna AS, Kuzhelev AA, Koval VV, Fedin MV, Godovikova TS, Bowman MK, and Bagryanskaya EG

Subjects
Electron Spin Resonance Spectroscopy, Hydrophobic and Hydrophilic Interactions, Molecular Structure, Spin Labels, Structure-Activity Relationship, Temperature, Indicators and Reagents chemistry, Mesylates chemistry, Serum Albumin, Human chemistry, Sulfhydryl Compounds chemistry
Abstract

Trityl radicals (TAMs) have recently appeared as an alternative source of spin labels for measuring long distances in biological systems. Finland trityl radical (FTAM) served as the basis for this new generation of spin labels, but FTAM is rather lipophilic and susceptible to self-aggregation, noncovalent binding with lipophilic sites of proteins, and noncovalent docking at the termini of duplex DNA. In this paper the very hydrophilic OX063 TAM with very low toxicity and little tendency for aggregation is used as the basis for a spin label. Human serum albumin (HSA) labeled with OX063 has an intense narrow line typical of TAM radicals in solution, whereas HSA labeled with FTAM shows broad lines and extensive aggregation. In pulse EPR measurements, the measured phase memory time T M for HSA labeled with OX063 is 6.3 μs at 50 K, the longest yet obtained with a TAM-based spin label. The lowered lipophilicity also decreases side products in the labeling reaction., (© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Published
2020
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