Search

Introduction. Researchers around the world are in search of new sources and drivers of economic development and growth. In this regard, digital economy is becoming one of the main internal factors stimulating the economic activity of the country and its regions. Therefore, its emergence, development and measurement are becoming a priority at present. Theoretical analysis. It is important to note that the formation and development of digital economy is associated with the main trends in economic development that have developed as a result of the third and fourth industrial revolutions. In this way, digital economy reflects the transition from the third to the fourth industrial revolution. Industrial revolutions are associated with a radical change in old technological modes and the emergence of completely new technologies that have a huge impact and contribute to the transformation of the economy and other areas. Empirical analysis. In the course of the study, the process of digital transformation was considered not only at the national level, but also at the level of the regions of the Russian Federation. It was revealed that digitalization within the country, in some regions, is extremely uneven. There is a small group of leading cities with a high level of digital development, and there are many regions that lag behind, in which the population has limited access to modern technologies. In addition to the gap in access to digital technologies and the Internet, there is an inequality in digital competencies, knowledge and skills of users, and ways of using technology. Results. In current reality, in order to ensure their development, competitiveness on the world stage, maintain sovereignty and national security, as well as to improve the standard of living and well-being of the population, states need to stimulate the digitalization of the economy and other spheres of society, approaching this comprehensively and systematically, develop roadmaps and national programs for the development of digital economy, follow their transition to digital economy and use foreign countries experience in the formation of its ecosystem. Their analysis will make it possible to compare and assess the level of digitalization in Russia, to develop recommendations for faster and higher quality digital development.

Dose-intensive cytostatic therapy and antibiotic treatment in allogeneic hematopoietic stem cell transplantation (allo-HSCT) cause severe abnormalities in a composition of gut microbiota as well as the emergence of antibiotic resistance. The data on the longitudinal recovery of major bacterial phyla and the expansion of genes associated with antibiotic resistance are limited. We collected regular stool samples during the first year after allo-HSCT from 12 adult patients with oncohematological disorders after allo-HSCT and performed 16SrRNA sequencing, multiplex PCR, conventional bacteriology and CHROMagar testing. We observed a decline in Shannon microbiota diversity index as early as day 0 of allo-HSCT (p = 0.034) before any administration of antibiotics, which persisted up to 1 year after transplantation, when the Shannon index returned to pre-transplant levels (p = 0.91). The study confirmed the previously shown decline in Bacillota (Firmicutes) genera and the expansion of E. coli/Shigella, Klebsiella and Enterococci. The recovery of Firmicutes was slower than that of other phyla and occurred only a year post-transplant. A positive correlation was observed between the expansion of E. coli/Shigella genera and blaKPC, blaCTX-M-1 and blaTEM (p < 0.001), Klebsiella spp. and blaOXA-48-like, blaNDM, blaCTX-M-1, blaTEM, and blaSHV (p < 0.001), Pseudomonas spp. and blaNDM (p = 0.002), Enterococcus spp. and blaOXA-48-like, blaNDM, blaCTX-M-1, blaSHV (p < 0.01). The correlation was observed between the expansion of Enterobacterales and and carbapenemase-positive CHROMagar samples (p < 0.001). Samples positive for carbapenem-resitant bacteria were at their maximum levels on day +30, and were gradually diminishing one year after allo-HSCT. From day +30 to +60, all isolated K. pneumoniae strains in fecal samples proved to be resistant to the main antibiotic groups (carbapenems, aminoglycosides, fluoroquinolones, third-generation cephalosporins). One year after HSCT, we documented the spontaneous decolonization of K. pneumoniae. The sensitivity of molecular biology techniques in the search for total and antibiotic-resistant Klebsiella seems to be superior to common bacteriological cultures. Future studies should be focused on searching for novel approaches to the efficient reconstitution and/or maintenance of strictly anaerobic microbiota in oncological patients. [ABSTRACT FROM AUTHOR]