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The biochar (BC) pyrolyzed from corn straw (CS), spent mushroom substrate (SMS) and hardwood sawdust (HS), which are activated by K2CO3, were utilized for lead (Pb) and cadmium (Cd) sorption in solution. The effects of different pyrolysis factors on the Pb and Cd sorption capacities of three types of K2CO3-activated biochar (ABC) were analyzed. A series of characterization analyses were applied to compare the differences between various ABCs and to explore the Pb and Cd removal mechanisms. The results showed that the pyrolysis temperature, residence time and K2CO3 concentration obviously affect the sorption capacity of all ABCs. According to characterization, SMS had a loosely porous structure, which might be more favorable for K2CO3 activation. Furthermore, SMS possessed a rich elemental composition. Therefore, the pyrolysis product derived from SMS exhibited a larger specific surface area and higher polar functional group content, which might be the reason why ABCs synthesized from SMS exhibited better Pb and Cd sorption capacities than did ABCs synthesized from CS or HS. Moreover, the contributions of different Pb or Cd sorption mechanisms by different ABCs decreased as follows: mineral precipitation > cation exchange > complexation of oxygen-containing functional groups > π-bonding or physical sorption. Overall, the ABC synthesized from SMS was more suitable for Pb or Cd removal in solution, which also provided a new approach for the comprehensive utilization of SMS. [ABSTRACT FROM AUTHOR]

Spent mushroom substrate (SMS) is a by-product remaining after harvesting mushrooms. We evaluated the effect of substituting chicken feed with 0–100% of Pleurotus eryngii and Lentinula edodes SMS at different stocking densities (200–1000 larvae/box) on development, composition, and substrate reduction of black soldier fly (Hermetia illucens) larvae. Although the survival rate was not significantly different, feeding pure SMS led to a low growth rate. The substitution level of SMS negatively correlated with individual larval weight, total harvested biomass, larval growth rate (LGR), feed conversion ratio (FCR), substrate reduction, and waste reduction index (WRI) except for the 20% substitution. Feeding 40% SMS resulted in the highest number of prepupae. In the density experiment, the heaviest larvae (220–239 mg fresh weight) were obtained at 200 larvae/box in the 0% SMS group. The frass residue and FCR decreased with increased density. Remarkably, when feeding 20% SMS at 250 larvae/box, the harvested biomass, LGR, and FCR did not differ significantly from the 0% SMS control, whereas some of the higher densities led to a deterioration. In fact, the frass residue, substrate reduction, and WRI were even improved at 250 larvae/box in the 20% SMS group. The chemical analyses of larvae reared on 20% SMS at 250 larvae/box showed comparable ash and fat contents and a higher protein content compared to the 0% SMS group. Accordingly, up to 20% of a standard diet such as chicken feed can be replaced by low-cost SMS without disadvantages for breeding. [ABSTRACT FROM AUTHOR]

Simple Summary: Pleurotus ostreatus spent mushroom substrate has high production but low utilization, and it is rarely fed to ruminants. This study evaluated the effects of the substrate on feed utilization and liver serum indices from the perspective of duodenal microorganisms. The results showed that the substrate had a complex effect on apparent digestibility, with the highest digestibility observed at the 10% replacement level. The addition of the substrate also influenced serum alanine aminotransferase levels. Although it did not affect the richness and diversity of duodenal microorganisms, the substrate was susceptible to contamination by Trichoderma, which could alter the intestinal microbiota structure. In conclusion, Pleurotus ostreatus spent mushroom substrate can be used in ruminant feeding, but its proportion should be controlled, with the optimal replacement level identified as 10% in this study. This study aimed to evaluate the effects of Pleurotus ostreatus spent mushroom substrate (P.SMS), which is characterized by high production but low utilization, on feed utilization and liver serum indices from the perspective of duodenal microorganisms. Forty-five 3-month-old Hu sheep were randomly assigned to five groups and fed diets in which whole-plant corn silage (WPCS) was substituted with P.SMS at levels of 0% (Con), 5% (PSMS5), 10% (PSMS10), 15% (PSMS15), or 20% (PSMS20). The results indicated that the addition of P.SMS complexly influenced the apparent digestibility of dry matter, organic matter, and crude protein, with PSMS10 showing the highest digestibility of these nutrients. P.SMS inclusion significantly affected serum alanine aminotransferase levels, with PSMS5 showing higher levels than both the Con and PSMS20 groups (p < 0.05). Importantly, the inclusion of P.SMS did not affect the richness and diversity of duodenal microorganisms. Significant differences in the phyla Verrucomicrobiota and Spirochaetota were observed between the Con and PSMS20 groups. The observed trend towards an increase in the genus Trichoderma (p = 0.057) suggests that P.SMS is susceptible to contamination by this genus, which in turn affects the structure of the intestinal flora. Furthermore, functional gene predictions indicated differences in amino acid metabolism among the groups (p < 0.05). In conclusion, feeding with 10% P.SMS resulted in the highest digestibility without adversely affecting the structure of the duodenal community or liver function. [ABSTRACT FROM AUTHOR]

Spent mushroom substrate (SMS), a lignocellulosic waste after mushroom production is generally discarded without proper management. There is increasing interest in the sustainable transformation of lignocellulosic waste into high-value products. Within this context, the present study investigated the potential of the SMS from the cultivation of Pleurotus pulmonarius and Auricularia auricula on rubber tree wood sawdust as substrates for xylooligosaccharides (XOS) production. SMS samples from these two edible mushrooms were extracted using alkaline xylan extraction, producing maximum true recovery amounts of xylan in the range 34.61%–37.49% using 18% NaOH at 70 °C for 3 h. Production of XOS from alkaline-extracted xylan from the SMS samples of both mushroom species using economically crude xylanases from Aspergillus flavus KUB2 resulted in XOS (X2–X5) production of 241.47–249.04 mg/g, with X3 as the predominant XOS product. The produced XOS had excellent prebiotic activity and 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and contained high total phenolic contents. The combined beneficial bioactivities in terms of prebiotic and antioxidant properties suggested that the XOS produced from sawdust-based SMS samples of P. pulmonarius and A. auricula could be promising ingredients for both food and pharmaceutical applications. [ABSTRACT FROM AUTHOR]

Simple Summary: This study explored the effects of incorporating spent mushroom substrate (SMS) from Flammulina velutipes as an exogenous enzyme in broiler diets. The objectives of this study were to evaluate the impact of being fed varying levels of SMS on the growth performance, carcass characteristics, meat quality, blood chemistry, cecal microbiota, and small intestinal morphology of broilers. The findings indicate that dietary supplementation with SMS significantly enhances growth performance, improves carcass characteristics, reduces serum cholesterol, and positively influences gut health. Supplementation with spent mushroom substrate (SMS) as an exogenous enzyme in broiler diets represents a promising nutritional strategy to enhance production efficiency and reduce costs. To assess the effects of SMS enzyme products on various parameters, including growth performance, blood chemistry, carcass and meat quality, and gut health, a study was conducted with one-day-old broilers (n = 500). The broilers were separated into five groups: a control diet (CON) and an antibiotic group (AGP) which were added to the diet at 0.25 g kg−1, and SMS from Flammulina velutipes supplementation groups which were added to the diet at 0.5 g kg−1 (SMS0.5), 1.0 g kg−1 (SMS1.0), and 2.0 g kg−1 (SMS2.0). Final body weight and average daily gain in broilers which were fed the AGP diet were higher than in broilers which were fed the CON, SMS0.5, and SMS1.0 diets. Broilers in the CON group had a decreased average daily feed intake compared to other groups. The addition of AGP and SMS2.0 diets improved the feed conversion ratio and reduced the feed cost per gain in broilers. Broilers that were fed the CON diet had the highest serum cholesterol, while the AGP diet increased triglyceride. Dietary supplementation of SMS improved some carcass characteristics and ceacum microbial content in broilers, especially with the SMS2.0 diet. Broiler fed CON and SMS0.5 worsened the villus height/crypt depth ratio of duodenum histology. In conclusion, SMS supplementation at 1.0 and 2.0 g kg−1 can improve productive performance and health in broiler diets; however, SMS2.0 had the best efficiency. [ABSTRACT FROM AUTHOR]

Introduction: The study aimed to evaluate the effects of Pleurotus Spent Mushroom Substrate (P.SMS) on the rumen microbiota, encompassing bacteria and fungi, as well as their interactions in Hu sheep. Methods: A total of forty-five 3-month-old Hu sheep were randomly assigned to five groups. Each group was fed diets in which whole-plant corn silage (WPCS) was substituted with P.SMS at varying levels: 0% (CON), 5% (PSMS5), 10% (PSMS10), 15% (PSMS15), or 20% (PSMS20). Results: The results indicated that higher proportions of P.SMS during the experimental period might have a detrimental effect on feed utilization efficiency, kidney function, and blood oxygen-carrying capacity. Notably, moderate levels of P.SMS, specifically below 15%, were associated with improvements in rumen NH3-N levels and absorption capacity. The results indicated that (1) PSMS20 exhibited a significantly higher feed-to-gain ratio compared to CON (P < 0.05); (2) PSMS15 showed a significantly higher NH3-N content than CON, PSMS5, and PSMS20. Additionally, PSMS10 and PSMS20 had elevated concentrations of NH3-N compared to CON and PSMS5 (P < 0.05); (3) The length and width of rumen papillae were significantly greater in PSMS20 compared to CON and PSMS5 (P < 0.05); (4) Creatinine levels were significantly higher in PSMS20 than in CON, PSMS5, and PSMS10 (P < 0.05); (5) By the conclusion of the experiment, hemoglobin concentration in PSMS20 showed a significant increase compared to CON (P < 0.05). Furthermore, the addition of P.SMS influenced microorganisms at both the phylum and genus levels: (1) At the phylum level, the prevalence of Patescibacteria was significantly lower in PSMS20 compared to the other groups; (2) PSMS15 exhibited significantly higher relative abundances of Basidiomycota compared to CON and PSMS10, while PSMS20 also demonstrated significantly higher relative abundances compared to CON (P < 0.05); (3) At the genus level, the prevalence of Candidatus_Saccharimonas in PSMS20 was significantly lower than in PSMS5, PSMS10, and PSMS15. Conversely, the prevalence of Phanerochaete in PSMS15 was notably higher than in CON and PSMS10, and it was also significantly elevated in PSMS20 compared to CON (P < 0.05); (4) Correlation analysis indicated no significant correlation between changes in the structure of bacterial and fungal communities. Discussion: Considering these findings, a high percentage of P.SMS negatively impacted feed utilization efficiency, blood oxygen carrying capacity, and kidney function, while a moderate percentage of P.SMS promotes rumen absorption capacity, indicating that feeding 10% P.SMS is optimal. [ABSTRACT FROM AUTHOR]

This study is the first to comprehensively investigate the potential of circular economy (CE) principles to enhance the sustainability of mushroom production, focusing on resource efficiency and waste reduction. A systematic literature review conducted in 2024 assessed the global state of CE applications in mushroom cultivation, emphasizing eight commonly cultivated species in Vietnam: Lentinula edodes, Ganoderma lucidum, Trametes versicolor, Hericium erinaceus, Cordyceps militaris, Auricularia heimuer, Pleurotus ostreatus, and Volvariella volvacea. The systematic literature review, covering 42 articles from Scopus and 46 from Web of Science, revealed a predominant focus on spent mushroom substrate (SMS) utilization, with limited attention to water recirculation, renewable energy, plastic recycling, and circular business models. In addition, we conducted 20 in-depth interviews with stakeholders across the mushroom production chain in the Central Highlands of Vietnam, identifying existing CE practices and opportunities for optimization. The study's novelty lies in its pioneering integration of systematic review and field study in the mushroom industry in the area. We propose CE recommendations for enhancing resource utilization and reducing environmental impact and greenhouse gas emissions in the agricultural sector. Our analysis showed that research on mushroom byproduct transformation largely focuses on SMS utilization for composting, ignoring other production processes such as the development of mushroom co-products at the output phase and plastic and water reuse during the production phase. Application of CE principles during the entire mushroom value chain and diversification of the use of mushroom waste and by-products are therefore necessary to provide additional benefits to mushroom production. [ABSTRACT FROM AUTHOR]

Abstract Spent mushroom substrate (SMS) is a by-product remaining after harvesting mushrooms. We evaluated the effect of substituting chicken feed with 0–100% of Pleurotus eryngii and Lentinula edodes SMS at different stocking densities (200–1000 larvae/box) on development, composition, and substrate reduction of black soldier fly (Hermetia illucens) larvae. Although the survival rate was not significantly different, feeding pure SMS led to a low growth rate. The substitution level of SMS negatively correlated with individual larval weight, total harvested biomass, larval growth rate (LGR), feed conversion ratio (FCR), substrate reduction, and waste reduction index (WRI) except for the 20% substitution. Feeding 40% SMS resulted in the highest number of prepupae. In the density experiment, the heaviest larvae (220–239 mg fresh weight) were obtained at 200 larvae/box in the 0% SMS group. The frass residue and FCR decreased with increased density. Remarkably, when feeding 20% SMS at 250 larvae/box, the harvested biomass, LGR, and FCR did not differ significantly from the 0% SMS control, whereas some of the higher densities led to a deterioration. In fact, the frass residue, substrate reduction, and WRI were even improved at 250 larvae/box in the 20% SMS group. The chemical analyses of larvae reared on 20% SMS at 250 larvae/box showed comparable ash and fat contents and a higher protein content compared to the 0% SMS group. Accordingly, up to 20% of a standard diet such as chicken feed can be replaced by low-cost SMS without disadvantages for breeding.

Lead (Pb) and cadmium (Cd)-contaminated wastewater treatment has become a major worldwide problem. The use of hydroxyapatite (HAP)-based composites may be an eco-friendly method for removing Pb and Cd from aqueous solutions. Based on the concept of treating waste with waste, we supported HAP particles on spent mushroom-substrate biochar (SMSBC), creating a new composite (HAP/BC) for removing Pb and Cd from solution. The sorption of Pb and Cd by HAP/BC was systematically studied in single and binary heavy metal systems. A series of characterization analyses were applied to investigate the properties of HAP/BC and explain the Pb and Cd removal mechanisms. The results revealed that the HAP content, reaction time, initial Pb and Cd concentrations, initial pH of the solution and competing cations affected Pb and Cd removal by HAP/BC. SMSBC effectively reduced HAP agglomeration, thereby resulting in HAP/BC with outstanding Pb and Cd removal capacities. The presence of Pb was not conducive to Cd removal, while the Pb removal efficiency was barely affected by the presence of Cd in the binary Pb–Cd system. Combining the results of the bath experiments with the characterization analyses, the reaction between HAP/BC and Pb might be primarily a dissolution–precipitation process, whereas the reaction with Cd was primarily an ion exchange. Additionally, oxygen-containing functional groups and cation-π interactions might also play a role in Pb and Cd sorption. Overall, the eco-friendly HAP/BC demonstrated significant potential for Pb and Cd decontamination and provided insights for the efficient use of SMS resources.Highlights: SMSBC use as a carrier could clearly reduce the clustering effect of HAP particles. HAP/BC exhibited superior Pb and Cd removal capacities in solution. Dissolution–precipitation dominated the reaction process between HAP/BC and Pb. The Cd removal process by HAP/BC was primarily controlled by ion exchange. [ABSTRACT FROM AUTHOR]

Spent mushroom substrate (SMS), a lignocellulosic waste after mushroom production is generally discarded without proper management. There is increasing interest in the sustainable transformation of lignocellulosic waste into high-value products. Within this context, the present study investigated the potential of the SMS from the cultivation of Pleurotus pulmonarius and Auricularia auricula on rubber tree wood sawdust as substrates for xylooligosaccharides (XOS) production. SMS samples from these two edible mushrooms were extracted using alkaline xylan extraction, producing maximum true recovery amounts of xylan in the range 34.61%–37.49% using 18% NaOH at 70 °C for 3 h. Production of XOS from alkaline-extracted xylan from the SMS samples of both mushroom species using economically crude xylanases from Aspergillus flavus KUB2 resulted in XOS (X2–X5) production of 241.47–249.04 mg/g, with X3 as the predominant XOS product. The produced XOS had excellent prebiotic activity and 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and contained high total phenolic contents. The combined beneficial bioactivities in terms of prebiotic and antioxidant properties suggested that the XOS produced from sawdust-based SMS samples of P. pulmonarius and A. auricula could be promising ingredients for both food and pharmaceutical applications.

Novel substrates consisted of different fresh agro-industrial residues, their corresponding and commercial spent mushroom substrates (i.e. SMS deriving from laboratory-scale experiments and SMS deriving from industrial-scale experiments by Green Zin S.A. - SMS GZ) and Pleurotus waste (PW; stipes/mishappen mushrooms) were used in blends for a new cultivation cycle of Pleurotus ostreatus and P. eryngii mushrooms in bags. Their impact on the biochemical properties (intra-cellular polysaccharides - IPSs, protein, lipid, total phenolic compounds - TPCs, individual carbohydrates composition of the IPSs) in the first- and second-flush whole mushrooms, pilei and stipes, as well as the fatty acids composition, the antioxidant activity (in the first-flush mushroom parts) and glucan content of stipes were examined. Both species produced satisfactory IPSs quantities in all substrates (28.69–46.38 %, w/w) and significant protein amounts (18.37–26.80 %, w/w). The further SMS addition (80 %, w/w instead of 40 %, w/w) in the cultivation substrates affected positively the mushroom IPSs values, whereas the highest protein content was detected in mushroom's parts cultivated on substrates consisted of fresh agro-industrial residues. Mushroom's lipid content was affected differently by the various substrate combinations, with SMS presence resulting in mushrooms with a lower fat content than those produced in substrates with PW addition. Fresh substrates with PW and those with coffee residue were the most favorable for TPCs production. Regarding production flushes, the nutritional value of mushrooms was comparable between them, only a slight increase in TPCs of second-flush carposomes was detected. Glucose was the predominant monosaccharide of the produced IPSs, combined with a significant production of total and β- glucans. SMSs and PW addition had a positive impact on antioxidant activity, too. A higher quantity of lipids, TPCs and significant antioxidant activity were detected in all Pleurotus pilei than stipes, whereas the latter were richer in IPSs. Both pilei and stipes had a significant protein amount. Hence, the data obtained by this study support the positive effect of different types of SMS and mushroom waste on P. ostreatus and P. eryngii nutritional value.

The high global market value of fresh mushrooms implies high importance of spent mushroom substrate (SMS) as a by-product that poses environmental challenges, if not properly treated. Reported studies demonstrate the positive effects of SMS on earthworm growth and reproduction, particularly when combined with other substrates such as cow dung (CD). This study utilized SMS and CD as substrates in varying ratios. A total of 375 young non-clitellated Eisenia fetida were randomly assigned to plastic containers, ensuring similar average individual worm weight, and maintained at 50% to 60% moisture. Weekly observations were made on earthworm average weight, cocoon production, and hatchling count. The growth and reproduction of earthworms differed significantly among treatments, with Treatment 3 (T3) (50:50 SMS and CD) showing the highest growth and Treatment 5 (T5) (0:100 SMS and CD) the lowest. Changes in pH, EC, total Kjeldahl nitrogen (TKN), total organic carbon (TOC), C/N ratio, and total available phosphorus (TAP) were observed over the experimental period, indicating dynamic nutrient dynamics within the substrates. The exchangeable potassium decreased with increasing proportions of CD, while the exchangeable sodium content showed variation across treatments. The observed variations can be attributed to differences in initial substrate composition and microbial activity during vermicomposting. [ABSTRACT FROM AUTHOR]

Besides other lignocellulose waste resulting from agriculture, spent mushroom substrate is a major concern due to the high amount of waste, usually improper disposed. Also, sand-based mortars require the use of this non-renewable and exhaustive aggregate and problems of environment protection arise. The study aims to analyze the possibilities of sand replacement in mortars composition with grounded substrate. The ratio between cement and sand in the studied compositions was 1:1, 1:2, and 1:4. The hardened mortars are analyzed in terms of colorimetric changes, density decrease, mechanical strength, hydrophobic properties, and freeze-frost resistance. Thus, medium-light mortars for applications that requires moderate strengths, with ecological implications results when up to 15% of sand is replaced with spent mushroom substrate. The results can be used for a deepen research, considering also a detailed life cycle assessment of the waste and, secondly, combining the replacement material with other waste types. [ABSTRACT FROM AUTHOR]

Novel substrates consisted of different fresh agro-industrial residues, their corresponding and commercial spent mushroom substrates (i.e. SMS deriving from laboratory-scale experiments and SMS deriving from industrialscale experiments by Green Zin S.A. - SMS GZ) and Pleurotus waste (PW; stipes/mishappen mushrooms) were used in blends for a new cultivation cycle of Pleurotus ostreatus and P. eryngii mushrooms in bags. Their impact on the biochemical properties (intra-cellular polysaccharides - IPSs, protein, lipid, total phenolic compounds - TPCs, individual carbohydrates composition of the IPSs) in the first- and second-flush whole mushrooms, pilei and stipes, as well as the fatty acids composition, the antioxidant activity (in the first-flush mushroom parts) and glucan content of stipes were examined. Both species produced satisfactory IPSs quantities in all substrates (28.69-46.38 %, w/w) and significant protein amounts (18.37-26.80 %, w/w). The further SMS addition (80 %, w/w instead of 40 %, w/w) in the cultivation substrates affected positively the mushroom IPSs values, whereas the highest protein content was detected in mushroom's parts cultivated on substrates consisted of fresh agroindustrial residues. Mushroom's lipid content was affected differently by the various substrate combinations, with SMS presence resulting in mushrooms with a lower fat content than those produced in substrates with PW addition. Fresh substrates with PW and those with coffee residue were the most favorable for TPCs production. Regarding production flushes, the nutritional value of mushrooms was comparable between them, only a slight increase in TPCs of second-flush carposomes was detected. Glucose was the predominant monosaccharide of the produced IPSs, combined with a significant production of total and ß- glucans. SMSs and PW addition had a positive impact on antioxidant activity, too. A higher quantity of lipids, TPCs and significant antioxidant activity were detected in all Pleurotus pilei than stipes, whereas the latter were richer in IPSs. Both pilei and stipes had a significant protein amount. Hence, the data obtained by this study support the positive effect of different types of SMS and mushroom waste on P. ostreatus and P. eryngii nutritional value. [ABSTRACT FROM AUTHOR]

The development of mushrooms may be hampered by municipal (MW) and garden wastes (GW) containing spent mushroom substrates (SMS) with high cellulose and lignin contents. In comparison to controls, Pleurotus flabellatus growth patterns were generally enhanced by SMS and/or GM treatments. Inductively coupled plasma-optical emission spectrometry (ICP-OES) and gas chromatography-mass spectrometry (GC-MS) were performed to characterize and identify 30 metabolites from P. flabellatus ethanolic extract. The synthesis of bioactive silver nanoparticles (PFE-AgNPs) from P. flabellatus was characterized using different instrumental methods. Spectral analysis revealed that biogenic PFE-AgNPs were spherically shaped and well-dispersed nanostructures with typical particle sizes ranging from 5 to 20 nm. Selective bacterial strains (25–100 μg/ml) were inhibited by PFE-AgNPs. The IC50 values for the antioxidant activity of facile PFE-AgNPs against DPPH and NOD were substantial (56.62 and 53.93 μg/ml). Furthermore, the anticancer effects of PFE-AgNPs at 10–100 μg/ml were tested on A549 cells after 24 and 48 h of exposure. The DNA damage assay revealed that A549 had more tail creation than the non-cancerous cell line. As a result, our study shows that PFE-AgNPs can be used to manage bacterial illnesses as well as lung cancer. The results indicated similar biological composition, and there is no aggregation of unwanted toxic and biological components that could be drawn from the waste-formulated substrate, ensuring that it is safe to use for sustainable mushroom growth. Additionally, the new and ecologically friendly PFE-AgNPs are enhanced by a technique that transforms municipal wastes into compounds with potential anti-bacterial and anti-cancer properties. [ABSTRACT FROM AUTHOR]

Organic fertilizers, such as spent mushroom substrate (SMS), improve soil fertility, but studies comparing their effects on different agricultural soils are limited. In this study, the effects of standard, SMS and composed fertilizers on soils from conventional–integrated, organic and biodynamic farming were investigated. Soil samples were analyzed for microorganisms and the activity of β-glucosidase (β-GLU), β-1,4-N-acetylglucosaminidase (NAG), urease (URE), arylamidase (ARN), phosphatase (PHOS), acid phosphatase (PAC), alkaline phosphatase (PAH) and arylsulphatase (ARS). Biodynamic soil showed the highest microbial counts and enzyme activities, followed by organic and conventional soils. SMS significantly increased the number of microorganisms and enzyme activities, especially in biodynamic and organic soils. Seasonal variations affected all microorganisms and most enzymes in all soils, except NAG in conventional and organic soils. Biodynamic soil showed stable activity of enzymes and microorganisms throughout the year, indicating greater stability. This study concludes that soil microorganisms and enzyme activities respond differently to fertilization depending on the soil type, with SMS demonstrating beneficial effects in all tested soils. [ABSTRACT FROM AUTHOR]

The study examined the impact of adding cattle manure to the composting process of Agaricus bisporus mushroom substrate on compost humification. A control group CK comprised entirely of Agaricus bisporus mushroom substrate, while the experimental group CD (70 percent Agaricus bisporus mushroom substrate and 30 percent cattle manure) comprised the two composting treatments that were established. The study determined that the addition of cow dung has promoted the formation of humus components. Particularly, humic substance (HS-C) and humic acid (HA) increased by 41.3 and 74.7%, respectively, and the ratio of humic acid to fulvic acid (HA/FA) also increased by 2.78. It showed that the addition of cow dung accelerated the synthesis and decomposition of precursors, such as polysaccharides, polyphenols, and reducing sugars. Thereby promoting the formation of humic acid. Network analysis revealed that adding cow dung promoted microbial interactions increased the complexity and stability of the bacterial and fungal symbiotic network, enhanced cooperation and reciprocity among microbes, and assisted in transforming fulvic acid (FA) components. Structural equation modeling (SEM) is a multivariate data analysis method for analyzing complex relationships among constructs and core indicators. SEM illustrated that introducing cattle manure into the composting process resulted in alterations to the correlation between physicochemical parameters and the microbial community, in addition to humus formation. Polysaccharides are the primary precursors for polymerization to form HA, which is an essential prerequisite for the conversion of fulvic acid to humic acid. Additionally, microbes affected the formation of humus, with bacteria substantially more influential than fungi. These findings provide new ideas for regulating the degree of humification in the composting process and have important practical implications for optimizing mushroom cultivation and composting techniques today. [ABSTRACT FROM AUTHOR]

Spent mushroom substrates (SMS) have been increasingly applied as organic fertilizer worldwide. However, the effects of various SMS on the accumulation and speciation characteristics of soil heavy metal(loid)s (HMs) are generally overlooked. Three types of SMS, including Flammulina velutipes residue (FVR), Agaricus bisporus residue (ABR), and Auricularia auricula residue (AAR), were applied to replace 25 % and 50 % of chemical fertilizer (based on nitrogen application) used in maize-wheat farmland. Compared to chemical fertilizer, the soil Cd, Pb, and As concentrations were decreased by 20.41 %, 5.97 %, and 10.09 %, respectively. And the residual fractions of soil HMs were increased through the application of SMS, indicating a reduction in their bioavailability. Notably, 50 % ABR replacement significantly increased the proportion of residual fraction in soil Cd, Pb, and As by 23.03 %, 15.15 %, and 4.85 %, respectively (P

Purpose:The inappropriate disposal of agricultural residues including spent mushroom substrate (SMS) after harvesting leads to serious environmental issues. Microbial composting is a widely recognized method for the management of such residues in a sustainable manner. Therefore, the present study aims to examine the efficacy of lignocellulolytic fungal consortium (Pseudoplagiostoma eucalypti strain LLF10 and Purpureocillium lilacinum strain LLF22) on the composting of SMS. Method: The experiment consisted of two treatments- T0: only SMS, and T1: SMS+ fungal accelerator solution (FAS) + Fungal consortium. The composting process lasted for 42 days and physicochemical parameters were assessed. Results: The results displayed that the fungal consortium accelerated SMS composting as evidenced by the decrease in the C:N ratio, an increase in macronutrient contents (TN, TK, TP, TCa, TMg), coarseness index (CI), and germination index (GI) of the final compost (T1) over the control (T0). Fourier transform infrared (FTIR) analysis revealed the breakdown of aromatic and organic compounds in the final compost suggesting humification of SMS. Furthermore, the inoculation of the fungal consortium effectively induced a faster rise in temperature and pH. Conclusion: This study revealed that the lignocellulolytic fungal consortium can be a potential candidate for the bioconversion of agro-waste into high-quality compost for application in sustainable agricultural practices. Research Highlighta • Fungal consortium manifested quality composting of spent mushroom substrate (SMS). • Fungal consortium induced an early increase in temperature and pH in T1 than in T0. • The compost of T1 showed C:N reduction and an increase in macronutrient contents. • FTIR analysis revealed the breakdown of lignocellulosic properties of the SMS. • Coarseness index (CI), and germination index (GI) were higher in T1 over the T0.

Currently, large quantities of spent mushroom substrate (SMS) are produced annually. Because SMS has high water retention and nutrients, it has great potential to replace traditional topsoil for raising seedlings in agricultural production. However, few studies have examined the effects of substituting SMS for paddy soil on rice seedling growth and soil nutrients. SMS was mixed with rice soil in different proportions (20%, 50%, and 80%), and chemical fertilizer, organic fertilizer, and peat substrate were added in addition to equivalent nitrogen as a traditional seedling nursery method for comparison. Compared to traditional paddy soil (CK), the seedling qualities of the three SMS ratio treatments were all higher. Adding SMS at different ratios promoted rice seedling root growth, elevated the soluble protein concentration, and amplified the superoxide dismutase (SOD) enzymatic action in rice seedlings. Total porosity and aeration porosity of the soil increased by 17.40% and 32.90%, respectively. Soil organic carbon (SOC), total nitrogen (TN), and total phosphorus (TP) increased by 21.26–118.48%, 50.44–71.68%, and 23.08–80.17%, respectively. Besides, the relative abundance of Bacillus, Bacteroidetes, and other bacteria as well as the abundance of Ascomycota were all significantly increased. Adding 50% SMS increased the abundance of Pseudomonas by 8.42 times. The seedling quality of the 50% SMS treatment was even higher than chemical fertilizer and organic fertilizer treatments, only second to the peat substrate treatment. In summary, partial substitution of paddy soil with SMS can ameliorate substrate properties, improve seedling quality, and increase microbial diversity, indicating the suitability of SMS as a replacement for rice soil in seedling substrates. The 50% SMS ratio is the best. This study provides a basis for SMS to replace traditional rice soil in seedling cultivation. [ABSTRACT FROM AUTHOR]

There is an overlooked problem which increasing microbial abundance while reducing nitrogen loss during composting. This study investigated the viability of spent mushroom substrate (SMS) as conditioners in the aerobic composting of kitchen waste (KW) with cattle manure (CM). The variation of temperature, pH, C/N, organic matter, cellulose, hemicelluloses, germination index (GI), and microflora structure were explored to evaluate the potential in accelerating maturity and nitrogen fixation by SMS addition. The results showed that the addition of SMS accelerated the heating rate, prolonged the high temperature time, and decreased organic matter, hemicellulose, and cellulose by 17.49%, 23.61%, and 18.62%, respectively. The GI reached 105.86% with SMS addition, while 74.17% was found in control treatment after composting. SMS changed the microbial community composition and increased the species abundance. Proteiniclasticum, Clostridium XI and Azomonas were dominant bacteria, which increased the retention of nitrogen, promoted organic matter degradation and reduce compost time. The study can provide a feasible strategy for nitrogen fixation in the field of organic waste recycling. [ABSTRACT FROM AUTHOR]

Spent mushroom substrate (SMS), often disregarded as waste despite its abundance in organic matter and mineral micronutrients, has emerged as a valuable resource for diverse applications. While Europe and Asia have witnessed extensive research in this field over the past decade, Ukraine's exploration remains limited. This study conducts a thorough investigation into SMS recycling and reutilization over a 4-year period. Employing experimental and comparative methods, this research unveils compelling insights into the potential of SMS for reintroduction into the primary production cycle and secondary activities. The main conclusions reveal the success of SMS valorization in the production of additives, fertilizers, and alternative fuels. Furthermore, the application of SMS in agroecosystems significantly enhances the soil biological activity. The integration of these methods into production chains not only yields economic benefits for companies but also fosters environmental stewardship, aligning with waste-free practices and the principles of bioeconomy and sustainability. [ABSTRACT FROM AUTHOR]

The aim of the present study was to investigate the effects of different dietary levels of spent mushroom substrate (SMS) at the expense of wheat straw (WS) on milk characteristics in dairy sheep. Thirty ewes at their final stage of lactation (145 ± 5 days after parturition) were randomly assigned into three groups; control (C), provided with a diet consisting of concentrates, alfalfa hay and WS, and SMS1 and SMS2, where WS was replaced by SMS at 50 or 100%, respectively. The experiment lasted for 28 days, and milk yield, composition, somatic cell count (SCC) and oxidative stability were monitored weekly, while milk fatty acid and immune cell profile were also determined on day 28. No significant differences were found in produced milk quantity, fat, protein, lactose, total solids non-fat, SCC and fatty acid profile between the experimental groups. However, milk oxidative stability was significantly improved as an effect of SMS (p < 0.001). At the same time, milk polymorphonuclear leukocyte percentage was decreased in SMS2 group (p < 0.05). As indicated, SMS seems to be a promising agro-industrial by-product for ewes' diet that could improve milk oxidative stability, without negatively affecting milk yield, composition and ewe health status, contributing in the context of sustainability, circular economy and protection of natural resources. [ABSTRACT FROM AUTHOR]

While spent mushroom substrate (SMS) has shown promise in increasing soil organic carbon (SOC) and improving soil quality, research on the interplay between SOC components and microbial community following the application of diverse SMS types remains scant. A laboratory soil incubation experiment was conducted with application of two types of SMSs from cultivation of Pleurotus eryngii (PE) and Agaricus bisporus (AB), each at three application rates (3, 5.5, and 8%). Advanced techniques, including solid-state 13C nuclear magnetic resonance (NMR) and high-throughput sequencing, were employed to investigate on SOC fractions and chemical structure, microbial community composition and functionality. Compared to SMS-AB, SMS-PE application increased the relative abundances of carbohydrate carbon and O-alkyl C in SOC. In addition, SMS-PE application increased the relative abundance of the bacterial phylum Proteobacteria and those of the fungal phyla Basidiomycota and Ascomycota. The relative abundances of cellulose-degrading bacterial (e.g., Flavisolibacter and Agromyces) and fungal genera (e.g., Myceliophthora, Thermomyces, and Conocybe) were increased as well. The application of SMS-AB increased the aromaticity index of SOC, the relative abundance of aromatic C, and the contents of humic acid and heavy fraction organic carbon. In addition, SMSAB application significantly increased the relative abundances of the bacterial phyla Firmicutes and Actinobacteria. Notably, the genera Actinomadura, Ilumatobacter, and Bacillus, which were positively correlated with humic acid, experienced an increase in relative abundance. Functional prediction revealed that SMS-PE application elevated carbohydrate metabolism and reduced the prevalence of fungal pathogens, particularly Fusarium. The application of highrate SMS-AB (8%) enhanced bacterial amino acid metabolism and the relative abundances of plant pathogenic fungi. Our research provides strategies for utilizing SMS to enrich soil organic carbon and fortify soil health, facilitating the achievement of sustainable soil management. [ABSTRACT FROM AUTHOR]

【Objective】The study aimed to investigate effects of rotating combined with spent mushroom substrate returning of Morchella esculenta (L.) Pers.on improving soil environment, yield and quality of flue-cured tobacco, and further rationally optimize planting system.【Method】Using the flue-cured tobacco variety Yunyan 87 as material, field experiments were carried out with two kinds of planting patterns including flue-cured tobacco-M.esculenta-flue-cured tobacco rotation (A1), flue-cured tobacco-winter leisure-flue-cured tobacco rotation (A2). In every rotation pattern, three application methods of M.esculenta spent mushroom substrate (AMMR) were used, including no AMMR (B1), mechanical rotary tillage (B2), pond (B3). Rhizosphere soil physical and chemical properties, microorganisms, plant growth, yield and chemical composition of flue-cured tobacco under the above various condition were compared.【Result】Soil physicochemical properties and leaf chemical composition could be controlled by planting pattern and AMMR. Of which, the interaction effect between the planting pattern and AMMR was the largest, followed by the single application of AMMR. Among them, the combination of A1B2, A1B3, A2B2 and A2B3 could effectively improve soil bulk density, porosity and large particle size aggregate content, and regulate soil CEC, pH and nutrients, additionally, A1B2 showed the most obvious effect. Compared with A1B1, the contents of soil organic matter, available N, P and K of A1B2 significantly increased by 22.71%, 5.07%, 20.94% and 32.55%, respectively. Moreover, comparing to A1B1 and A2B1, the combination of A1B2, A1B3, A2B2 and A2B3 significantly increased number of soil fungi, bacteria and actinomyces by 1.13-2.93 times. The contents of carbon, nitrogen, and ratio of carbon and nitrogen of microbial biomass increased by 42.19%-77.46%, 25.68%-59.97% and 3.55%-28.99%, respectively. It could significantly improve leaf area, proportion of high-grade tobacco, yield and output value. Among them, the output value of A1B2 had the largest increase, significantly increasing by 15.05% and 29.12% respectively compared with the output value of A1B1 and A2B1.The regulatory effect of each factor on the availability index of chemical components in tobacco leaves was as follows: A×B interaction＞AMMR＞planting pattern. Compared with A1B1 and A2B1, A1B2, A1B3, A2B2 and A2B3 significantly reduced total sugar and total nitrogen, and increased potassium content. Moreover, the contents of total nitrogen and nicotine decreased, while the contents of potassium increased. The planting pattern mainly affected total nitrogen and potassium content of tobacco leaves.【Conclusion】Using the planting mode of flue-cured tobacco-M.esculenta-flue-cured tobacco combing with mechanical rotary tillage application of AMMR is beneficial to improve soil permeability and aggregate structure, enhance soil fertility, increase soil microbial community abundance, promote the leaf growth, yield, output value of flue-cured tobacco, and the leaf chemical composition is more coordinated. [ABSTRACT FROM AUTHOR]

Pleurotus ostreatus (Jacq. ex Fr.) P. Kumm mushrooms are cultivated on diverse by-products based on substrates that hold promise for mitigating antibiotic usage in the poultry industry and reducing environmental pollution. By incorporating agricultural by-products into mushroom cultivation, the functionality of the mushroom products can be increased, then the final product can be a more effective feed supplement. After mushroom cultivation, spent mushroom substrate (SMS) can be valorized, due to the presence of huge amounts of bioactive compounds like β-glucan, chitin, polyphenols, and flavonoids related to mycelia. As a prebiotic and antimicrobial feed supplement, these mushrooms positively influence gut microbiota, intestinal morphology, and thus overall poultry well-being. This article underscores the potential of solid-state fermentation (SSF) to enhance the bioactivity of oyster mushrooms and their derivatives, offering a cost-effective and efficient strategy for transforming unconventional feeding materials. Moreover, it emphasizes broader implications, including the reduction of antibiotic dependence in poultry farming, highlighting the promising integration of oyster mushrooms and their derivatives for sustainable and environmentally conscious poultry production. [ABSTRACT FROM AUTHOR]

In this work we explore the use of brewers' spent grain to produce biogas after it was used to produce the edible mushroom Pleurotus pulmonarius within the concept of circular economy. We compare the production of biomethane obtained using: (i) spent mushroom substrate and (ii) brewers' spent grain to feed the digester. We produced the mushroom using a mixture of sawdust and brewers' spent grain and the resulting spent mushroom substrate was used to feed the digester. The mono-digestion of both substrates was carried out in 19 l semi-continuos biodigesters, in mesophilic conditions (35 °C), constant stirring of 200 rpm and a hydraulic retention time of 30 days. The start-up of biodigesters was carried out in stages. PH, volume and biogas composition were recorded daily. Accumulated biogas volumes of 59.97 l and 465.73 l were obtained for the spent mushroom substrate and brewers' spent grain respectively. The yield and average methane composition produced were 35.95 l CH4/kgVS and 42% for spent mushroom substrate; while for brewers' spent grain 265.495 l CH4/kgVS and 55% were obtained. The use of brewers' spent grain, first as substrate for mushroom production, and then, using spent mushroom substrate to produce biogas would not be a recommended practice if the main objective were the production of biogas. Since it is much more efficient to produce biomethane when brewers' spent grain is used directly. The results obtained from the use of brewers' spent grain show that this substrate improves the yield of mushroom production and can be used for the production of biogas in semi-continuos biodigesters with a high performance. Statement of Novelty: Brewery spent grain (BSG) significantly increased the production of P. pulmonarius. The SMS formulated with BSG produced low biogas volumes in a semi continuous reactor. The methane yield with BSG was high compare to the substrates used nowadays to produce biogas. [ABSTRACT FROM AUTHOR]

IntroductionThe study aimed to evaluate the effects of Pleurotus Spent Mushroom Substrate (P.SMS) on the rumen microbiota, encompassing bacteria and fungi, as well as their interactions in Hu sheep.MethodsA total of forty-five 3-month-old Hu sheep were randomly assigned to five groups. Each group was fed diets in which whole-plant corn silage (WPCS) was substituted with P.SMS at varying levels: 0% (CON), 5% (PSMS5), 10% (PSMS10), 15% (PSMS15), or 20% (PSMS20).ResultsThe results indicated that higher proportions of P.SMS during the experimental period might have a detrimental effect on feed utilization efficiency, kidney function, and blood oxygen-carrying capacity. Notably, moderate levels of P.SMS, specifically below 15%, were associated with improvements in rumen NH3-N levels and absorption capacity. The results indicated that (1) PSMS20 exhibited a significantly higher feed-to-gain ratio compared to CON (P

This study aimed to evaluate the effects of Pleurotus ostreatus spent mushroom substrate (P.SMS), which is characterized by high production but low utilization, on feed utilization and liver serum indices from the perspective of duodenal microorganisms. Forty-five 3-month-old Hu sheep were randomly assigned to five groups and fed diets in which whole-plant corn silage (WPCS) was substituted with P.SMS at levels of 0% (Con), 5% (PSMS5), 10% (PSMS10), 15% (PSMS15), or 20% (PSMS20). The results indicated that the addition of P.SMS complexly influenced the apparent digestibility of dry matter, organic matter, and crude protein, with PSMS10 showing the highest digestibility of these nutrients. P.SMS inclusion significantly affected serum alanine aminotransferase levels, with PSMS5 showing higher levels than both the Con and PSMS20 groups (p < 0.05). Importantly, the inclusion of P.SMS did not affect the richness and diversity of duodenal microorganisms. Significant differences in the phyla Verrucomicrobiota and Spirochaetota were observed between the Con and PSMS20 groups. The observed trend towards an increase in the genus Trichoderma (p = 0.057) suggests that P.SMS is susceptible to contamination by this genus, which in turn affects the structure of the intestinal flora. Furthermore, functional gene predictions indicated differences in amino acid metabolism among the groups (p < 0.05). In conclusion, feeding with 10% P.SMS resulted in the highest digestibility without adversely affecting the structure of the duodenal community or liver function.

Supplementation with spent mushroom substrate (SMS) as an exogenous enzyme in broiler diets represents a promising nutritional strategy to enhance production efficiency and reduce costs. To assess the effects of SMS enzyme products on various parameters, including growth performance, blood chemistry, carcass and meat quality, and gut health, a study was conducted with one-day-old broilers (n = 500). The broilers were separated into five groups: a control diet (CON) and an antibiotic group (AGP) which were added to the diet at 0.25 g kg−1, and SMS from Flammulina velutipes supplementation groups which were added to the diet at 0.5 g kg−1 (SMS0.5), 1.0 g kg−1 (SMS1.0), and 2.0 g kg−1 (SMS2.0). Final body weight and average daily gain in broilers which were fed the AGP diet were higher than in broilers which were fed the CON, SMS0.5, and SMS1.0 diets. Broilers in the CON group had a decreased average daily feed intake compared to other groups. The addition of AGP and SMS2.0 diets improved the feed conversion ratio and reduced the feed cost per gain in broilers. Broilers that were fed the CON diet had the highest serum cholesterol, while the AGP diet increased triglyceride. Dietary supplementation of SMS improved some carcass characteristics and ceacum microbial content in broilers, especially with the SMS2.0 diet. Broiler fed CON and SMS0.5 worsened the villus height/crypt depth ratio of duodenum histology. In conclusion, SMS supplementation at 1.0 and 2.0 g kg−1 can improve productive performance and health in broiler diets; however, SMS2.0 had the best efficiency.

In the present study, biological indicators were used to assess the impact of applying spent mushroom substrate and manure on the soil environment. The use of spent mushroom substrate had a varied effect on the microorganisms. Stimulation was recorded in the abundance of copiotrophic bacteria and fungi, but only in the first year of the study. In the case of cellulolytic bacteria, this effect was visible only in single plots. Similar observations were also noted regarding the relative DNA content (in relation to the control), which increased for both bacteria and fungi after applying spent mushroom substrate. In the soil fertilized with spent mushroom substrate, a decrease in DNA concentration was observed, but only in the first and second year. For enzymatic activity, the use of spent mushroom substrate alone proved to be more favorable, but this effect was again observed only in the first year of the study. The application of manure caused similar changes as observed with the use of spent mushroom substrate. These observations indicate a similar impact of spent mushroom substrate and manure on the parameters tested. The research presented suggests the use of both classical methods and methods based on the analysis of DNA extracted from soil to study the impact of spent mushroom substrate on the activity of soil microbial populations. [ABSTRACT FROM AUTHOR]

Introduction: Spent mushroom substrate (SMS) is a solid waste in agricultural production that contains abundant lignocellulosic fibers. The indiscriminate disposal of SMS will lead to significant resource waste and pollution of the surrounding environment. The isolation and screening of microorganisms with high cellulase degradation capacity is the key to improving SMS utilization. Methods: The cellulose-degrading microbial consortiums were constructed through antagonism and enzyme activity test. The effect of microbial consortiums on lignocellulose degradation was systematically evaluated by SMS liquid fermentation experiments. Results: In this study, four strains of cellulose-degrading bacteria were screened, and F16, F, and F7 were identified as B. amyloliquefaciens, PX1 identified as B. velezensis. At the same time, two groups of cellulose efficient degrading microbial consortiums (PX1 + F7 and F16 + F) were successfully constructed. When SMS was used as the sole carbon source, their carboxymethyl cellulase (CMCase) activities were 225.16 and 156.63 U/mL, respectively, and the filter paper enzyme (FPase) activities were 1.91 and 1.64 U/mL, respectively. PX1 + F7 had the highest degradation rate of hemicellulose and lignin, reaching 52.96% and 52.13%, respectively, and the degradation rate of F16 + F was as high as 56.30%. Field emission scanning electron microscopy (FESEM) analysis showed that the surface microstructure of SMS changed significantly after microbial consortiums treatment, and the change of absorption peak in Fourier transform infrared spectroscopy (FTIR) and the increase of crystallinity in X-ray diffraction (XRD) confirmed that the microbial consortiums had an actual degradation effect on SMS. The results showed that PX1 + F7 and F16 + F could effectively secrete cellulase and degrade cellulose, which had practical significance for the degradation of SMS. Discussion: In this study, the constructed PX1 + F7 and F16 + F strains can effectively secrete cellulase and degrade cellulose, which holds practical significance in the degradation of SMS. The results can provide technical support for treating highcellulose solid waste and for the comprehensive utilization of biomass resources. [ABSTRACT FROM AUTHOR]

Commercial cultivation of edible mushrooms utilizes a large amount of lignocellulosic material as a substrate. After harvest, the leftover substrate, referred to as a spent mushroom substrate (SMS), largely remains biochemically unaltered. In light of the global rise in the number of edible mushroom production facilities, it is pertinent to sustainably manage the by‐products of the mushroom production process, particularly SMS. Following the principles of the circular economy, SMS has shown potential for a variety of applications: SMS can be used as substrate for a new cycle of mushroom cultivation or as animal feed, fertilizer, soil amendment, bioremediation agent or a substrate for renewable energy production such as biogas, bioethanol, biohydrogen, solid biofuel, bio‐crude and bio‐oil. This article summarizes the current state of knowledge in various applications, highlights recent developments in the field, discusses potential barriers and offers suggestions for the long‐term growth of the mushroom industry worldwide. [ABSTRACT FROM AUTHOR]

Spent mushroom substrate (SMS) is an important by-product of the mushroom industry having tremendous potential as a bio-fertilizer, soil amendment, plant protectant, animal feed, and renewable energy source. An investigation was performed to examine its microbial diversity and potentiality in sesame root rot management in association with antagonistic microbial inoculants in vitro. The investigation revealed that SMS gathers different fungal genera such as Penicillium, Rhizopus, Aspergillus, Trichoderma, and Pleurotus which were found antagonists to Macrophomina phaseolina in dual culture. In pot culture, Trichoderma-enriched SMS promoted germination (86.67%), suppressed the disease severity up to an appreciable limit (68.86%), and promoted plant growth such as plant heights (45.67 cm), fresh weight (4.02 g), dry weight (1.18 g), number of capsules (9.0) as compared to others. In addition, GC–MS analysis of ethyl acetate fractions of culture filtrates of Trichoderma asperellum showed the abundant presence of antifungal volatile compound 6-Pentyl-2 H-pyran-2-one (22.02%). FTIR spectra of SMS in comparison to unused straw substrate revealed the presence of different functional groups like water, alcohols, phenols, alkynes, amide, amines, and alkyl halides having different stretching. These findings indicate that SMS has the potential to suppress root rot of sesame and augment plant growth in association with the fungal antagonist T. asperellum. [ABSTRACT FROM AUTHOR]

Lignocellulose biomasses (LCB), including spent mushroom substrate (SMS), pose environmental challenges if not properly managed. At the same time, these renewable resources hold immense potential for biofuel and chemicals production. With the mushroom market growth expected to amplify SMS quantities, repurposing or disposal strategies are critical. This study explores the use of SMS for cultivating microbial communities to produce carbohydrate-active enzymes (CAZymes). Addressing a research gap in using anaerobic digesters for enriching microbiomes feeding on SMS, this study investigates microbial diversity and secreted CAZymes under varied temperatures (37 °C, 50 °C, and 70 °C) and substrates (SMS as well as pure carboxymethylcellulose, and xylan). Enriched microbiomes demonstrated temperature-dependent preferences for cellulose, hemicellulose, and lignin degradation, supported by thermal and elemental analyses. Enzyme assays confirmed lignocellulolytic enzyme secretion correlating with substrate degradation trends. Notably, thermogravimetric analysis (TGA), coupled with differential scanning calorimetry (TGA-DSC), emerged as a rapid approach for saccharification potential determination of LCB. Microbiomes isolated at mesophilic temperature secreted thermophilic hemicellulases exhibiting robust stability and superior enzymatic activity compared to commercial enzymes, aligning with biorefinery conditions. PCR-DGGE and metagenomic analyses showcased dynamic shifts in microbiome composition and functional potential based on environmental conditions, impacting CAZyme abundance and diversity. The meta-functional analysis emphasised the role of CAZymes in biomass transformation, indicating microbial strategies for lignocellulose degradation. Temperature and substrate specificity influenced the degradative potential, highlighting the complexity of environmental–microbial interactions. This study demonstrates a temperature-driven microbial selection for lignocellulose degradation, unveiling thermophilic xylanases with industrial promise. Insights gained contribute to optimizing enzyme production and formulating efficient biomass conversion strategies. Understanding microbial consortia responses to temperature and substrate variations elucidates bioconversion dynamics, emphasizing tailored strategies for harnessing their biotechnological potential. [ABSTRACT FROM AUTHOR]

The remediation of mixed contaminated soil is challenging as it often requires actions to minimize metal-induced risks while degrading organic contaminants. Here, the effectiveness of different bioremediation strategies, namely, rhizoremediation with native plant species, mycoremediation with Pleurotus ostreatus spent mushroom substrate, and biostimulation with organic by-products (i.e., composted sewage sludge and spent mushroom substrate), for the recovery of a mixed contaminated soil from an abandoned gravel pit was studied. The combination of biostimulation and rhizoremediation led to the most significant increase in soil health, according to microbial indicator values. The application of composted sewage sludge led to the highest reduction in anthracene and polychlorinated biphenyls concentrations. None of the strategies managed to decrease contamination levels below regulatory limits, but they did enhance soil health. It was concluded that the biological remediation treatments improved soil functioning in a short time, before the concentration of soil contaminants was significantly reduced. [ABSTRACT FROM AUTHOR]

Simple Summary: Spent Hypsizygus marmoreus substrate (SHMS) is a byproduct of the Hypsizygus marmoreus harvest and contains numerous nutrients and active substances. It is a new type of high-quality feed material for livestock and poultry, with a certain application value. The objective of this study was to investigate the effects of composite alkali-stored SHMS on carcass quality, rumen fermentation, and rumen microbial diversity in goats. The results showed that adding it to the diet improved the carcass quality. It also increased the content of the total volatile fatty acids in rumen and had a positive impact on rumen fermentation. Furthermore, it altered the rumen microbial community structure of the goats. These research findings can provide scientific references for the utilization of SHMS as feed in the goat industry. The objective of this study was to investigate the effects of composite alkali-stored spent Hypsizygus marmoreus substrate (SHMS) on carcass quality, rumen fermentation, and rumen microbial diversity in goats. Twenty-four 6-month-old Chuanzhong black goats with similar body weights (20 ± 5 kg) were selected and randomly divided into four groups (n = 6 per group) and received four treatments: 0% (control group, CG); 20% (low-addition group, LG); 30% (moderate-addition group, MG); and 40% (high-addition group, HG) of SHMS-replaced silage corn and oat hay. The experiment lasted for 74 days (including a 14 d adaptation period and a 60 d treatment period). The results of this study showed that MG and HG significantly improved the marble score of goat meat (p < 0.05). The flesh color score significantly increased in each group (p < 0.05). The fat color scores significantly increased in LG and MG (p < 0.05). There were no significant effects on the pH value or shear force of the longissimus dorsi in each group (p > 0.05). The cooking loss in MG was higher than that in CG (p < 0.05). The histidine and tyrosine contents in each group of muscles significantly increased (p < 0.05), with no significant effect on fatty acids (p > 0.05). The rumen pH of MG significantly decreased (p < 0.05), while the total volatile fatty acids (TVFAs) and ammoniacal nitrogen (NH3-N) increased by 44.63% and 54.50%, respectively. The addition of the SHMS altered both the alpha and beta diversities of the rumen microbiota and significant differences in the composition and structure of the four microbial communities. The dominant bacterial phylum in each group were Firmicutes and Bacteroidetes, with Prevotella 1 as the dominant bacterial genus. Correlation analysis revealed that rumen bacteria are closely related to the animal carcass quality and rumen fermentation. In the PICRUSt prediction, 21 significantly different pathways were found, and the correlation network showed a positive correlation between the Prevotella 1 and 7 metabolic pathways, while the C5-branched dibasic acid metabolism was positively correlated with nine bacteria. In summary, feeding goats with an SHMS diet can improve the carcass quality, promote rumen fermentation, and alter the microbial structure. The research results can provide a scientific reference for the utilization of SHMS as feed in the goat industry. [ABSTRACT FROM AUTHOR]

The global market for mushrooms is growing due to its nutritional enrichment, potential usage as a bioremediation, enzyme production, and functional food development. However, the leftover post-harvest mushroom substrate (SMS) generates certain environmental concerns. This study aimed to investigate the potential of SMS obtained from two oyster mushroom species—Pleurotus ostreatus and Pleurotus djamor. These were examined regarding sustainability by analyzing their lignocellulosic enzyme production, cellulose yield, antimicrobial properties, and proximate composition. The findings for both P. ostreatus and P. djamor showed higher activity of amylase, that is, 0.3 U (μmol/min) and 0.7 U (μmol/min), respectively, compared to activity of cellulase, which showed 0.3 U (μmol/min) and 0.5 U (μmol/min), respectively. SMS showed the highest activity of lignocellulosic enzymes, compared to non-SMCs and controls at p ≤ 0.00 and ≤0.01), proving fungual mycelia as the precursor of enzymes activity, as no mushroom is cultivated due to least enzymatic activity. The results for proximate analysis of SMCs showed a significant difference from non-SMCs. The findings for P. djmor revealed protein (1.23%), fats (1.3%), and ash (8.11), which were significantly higher than in P. ostreatus. A positive co-relation of 52% was established between SMCs with amylase, while a correlation of 20% was observed with cellulase, depicting an impact of mycelia in the breakdown of protein for amylase production. The SMC samples were also subjected to antibacterial analysis against Staphylococcus aureus, E. coli, and Xanthomonas. A higher minimum inhibition concentration (MIC) was recorded for P. djamor, that is, 8.80 mm, 11.66 mm, and 9.04 mm, compared to P. ostreatus, which showed its highest MIC as 9.18 mm, 9.30 mm, and 9.28 mm for S. aureus, E. coli, and Xanthomonas, respectively. It was evident from the study that SMC has a potential of being utilized for bioremediation, as it is therapeutically active against pathogens. Additionally, Pleurotus spp. is of great interest because of its ability to produce high nutritive value, cellulose yield, and a vast amount of lignocellulosic enzymes. The current experiment recommends the use of distilled water for mushroom farming, as enzymatic activities can significantly be affected by pH and buffers. Furthermore, the spent compost, being rich nutritionally, can be used for soil enrichment or as a biofertilizer. [ABSTRACT FROM AUTHOR]

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This article provides an overview of the global mushroom market and its potential for growth. It highlights China as a major player in the market, with their low-priced mushrooms posing challenges for Brazilian producers. The article suggests that Brazil could increase its competitiveness by integrating the mushroom industry with other agri-food industries, particularly through the use of spent mushroom substrate (SMS) in a circular economy model. It also discusses the increasing cultivation of Shiitake, Shimeji, and Black ear mushrooms, and the opportunities for Brazil in the "In natura" segment. The article emphasizes the importance of reducing production costs and minimizing environmental impacts in order to succeed in the mushroom market. [Extracted from the article]

Lentinula edodes, commonly known as shiitake mushroom, is renowned for its potential health advantages. This research delves into the often-overlooked by-product of shiitake cultivation, namely spent mushroom substrate (SMS), to explore its nutraceutical properties. The SMS samples were collected and subjected to different extraction methods, namely short or long agitation, and ultrasound-assisted extractions using different temperatures and distilled water or a 50% (v/v) ethanol as solvents. The extracts were tested for phenolic content (total phenols, ortho-diphenols, and flavonoids), antioxidant capacity (DPPH, 2,2-diphenyl-1 picrylhydrazyl; ABTS, 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid; and FRAP, ferric reducing antioxidant power), and antibacterial activity. The different extraction methods revealed substantial variations (p < 0.05) in phenolic composition and antioxidant capacity. The highest phenolic content and antioxidant capacity were achieved using 24 h extraction, agitation, 50 °C, and ethanol as the solvent. Furthermore, the extracted compounds displayed antibacterial activity in specific tested bacterial strains. This study highlights the nutraceutical potential of L. edodes' SMS, positioning it as a valuable dietary supplement for animal nutrition, with emphasis on its prebiotic properties. Hence, this research unveils the promising health benefits of SMS in both human and animal nutrition. [ABSTRACT FROM AUTHOR]

Nowadays, agricultural by-product disposal is a major concern. The mushroom by-products could be used as an alternative feed source in rabbit diets. Therefore, partial replacement of berseem hay (BH) with spent mushroom substrate (SMS) was conducted in four experimental groups as follows: 0, 20, 40, and 60% of SMS. Forty weaned New Zealand White (NZW) rabbits at 6th weeks of age with an initial body weight of 520.25 ± 70.01 g were fed the experimental diet for 8 weeks. The results showed that ash content and cell wall constituents in SMS were higher than in BH, while the other nutrient compounds in SMS were lower than in BH. Dietary SMS at a level of 60% increased the final body weight (p = 0.05) and feed conversion ratio (p ≤ 0.05). However, average daily gain and total feed intake were not affected by treatments. The replacement of SMS at levels of 40 and 60% significantly improved nutrient digestibility and total digestible nutrient value. SMS 60% decreased (p = 0.040) faecal N and improved (p = 0.006) retained nitrogen. The SMS replacement increased caecum length (p = 0.001), and full and empty caecum weight (p = 0.001 and 0.021, respectively) compared to the control. The SMS inclusion caused a decrease (p = 0.021 and 0.007) in the pH and NH3-N concentrations, respectively. Total VFA, acetic acid, butyric acid, and propionic acid proportions increased with the dietary inclusion of SMS in a level-dependent manner. Using SMS as a replacement for BH in growing rabbit diets reduced the total feed cost, and consequently improved net revenue, economic efficiency, and relative economic efficiency. [ABSTRACT FROM AUTHOR]