[Objective] The effects of quinoa sowing date and density on soil nutrients, enzyme activities and microbial community structure were analyzed, and the optimal sowing date and sowing density of quinoa in Gannan area were determined, aiming to provide a theoretical basis and scientific reference for the adjustment of quinoa cultivation system and the promotion of quinoa industry. [Method] Using quinoa variety 'Longquinoa No. 5' as the material, a two-factor random block design was adopted, and two factors were set, including early sowing (sowing on May 15, E) and late sowing (sowing on June 24, L), and low density (1.2X104 plants/hm², D1), medium density (1.6X104 plants/hm², D2) and high density (2.0X104 plants/hm², D3), a total of 6 treatments, namely early sowing low density (ED1), early sowing medium density (ED2), early sowing high density (ED3), late sowing low density (LD1), late sowing medium density (LD2) and late sowing low density (LD3), the yield and its constituent factors of different treatments of quinoa were analyzed, and the rhizosphere soil pH of different treatments was determined. Nutrient content and soil enzyme activity, Illumina-HiSeq high-throughput sequencing technology was used to analyze the composition and diversity of bacterial community in rhizosphere soil of quinoa under different treatments, and finally the correlation between soil pH and nutrient content, relative abundance of bacterial community and enzyme activity was analyzed. [Result] Sowing date and density had significant effects on quinoa yield and its constituent factors. There was no significant difference in main panicle length among the treatments. Among the six treatments, the effective branch number and 1 000-grain weight of quinoa in LD1 treatment were the highest, and the total yield of ED2 and ED3 treatments was higher. Sowing date and density had no significant effect on soil pH in the rhizosphere of quinoa, but had a significant effect on soil nutrient content and enzyme activity. At the same density, the contents of total phosphorus, total potassium, total nitrogen and organic matter in the rhizosphere soil of late sowing quinoa were generally higher than those in the early sowing treatment, both at the branching stage and at the grain-filling stage. At the same density, the activities of catalase, sucrase and urease in the rhizosphere soil of late sowing quinoa at the branching stage were generally higher than those in the early sowing treatment, while the alkaline phosphatase in the early sowing treatment was lower than that in the late sowing treatment, and the catalase and alkaline phosphatase activities in the rhizosphere soil of late sowing quinoa at the grain filling stage were generally lower than those in the early sowing treatment, and the sucrase activities in the late sowing treatment were higher than those in the early sowing treatment. Sowing date and density had significant effects on the bacterial community structure of quinoa rhizosphere soil.In the branching stage, the average Shannon index and Simpson index of ED2 treatment were the highest, and the Chao1 index of LD2 treatment was the highest, and the coverage index of ED3 treatment was the highest. The results of correlation analysis showed that soil nutrient content and enzyme activity were significantly correlated with soil bacterial community structure, among which soil urease activity was significantly positively correlated with available phosphorus content, catalase activity was significantly positively correlated with available potassium content, soil sucrase was significantly positively correlated with total nitrogen content, catalase activity was significantly positively correlated with actinomycetes, urease activity was significantly positively correlated with Proteobacteria, and sucrase activity was significantly positively correlated with Blastomonas. [Conclusion] In the high-altitude cold areas of Gansu Province, the sowing date and density had significant effects on the yield of quinoa, its rhizosphere soil nutrient content, enzyme activity and bacterial community, and the sowing time of quinoa in this area was mid-May and the sowing density was (1.6-2.0)X104 plants/hm². [ABSTRACT FROM AUTHOR]