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5. Real-world effectiveness of original BNT162b2 mRNA COVID-19 against symptomatic Omicron infection among children 5–11 years of age in Brazil: A prospective test-negative design study

Author

Rodrigues, Cristina de Oliveira, Spinardi, Julia, Rosa, Regis Goulart, Falavigna, Maicon, de Souza, Emanuel Maltempi, Manfio, Josélia Larger, de Souza, Ana Paula, de Araujo, Cintia Laura Pereira, Cohen, Mírian, Barbosa, Gynara Rezende Gonzalez do Valle, Silva, Fernanda Kelly Romeiro, Sganzerla, Daniel, da Silva, Mariana Motta Dias, Ferreira, Diogo, Kunkel, Nicolas Taciano, Camargo, Nathan Iori, Sarturi, Jean Carlos, Guilhem, Márcia Cristina, de Oliveira, Jaqueline Carvalho, Lopes, Caroline Cardoso, Widmar, Fernanda, Barufi, Letícia Killes, da Silva, Gabrielle Nunes, Gradia, Daniela Fiori, Brandalize, Ana Paula Carneiro, Royer, Carla Adriane, Luiz, Rafael Messias, Baura, Valter Antonio, Abreu, Hellen, Poitevin, Carolina Gracia, Kucharski, Gabriela Almeida, Pedrotti, Fernando, Valluri, Srinivas Rao, Srivastava, Amit, Julião, Viviane Wal, Melone, Olga Chameh, Allen, Kristen E., Kyaw, Moe H, Castillo, Graciela del Carmen Morales, and McLaughlin, John M

Published
2024
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9. The use of Arduino as a methodological tool for teaching physics in high school

Author

de Lima, Ítalo M., de Souza, Emanuel V., and de Araújo, Francisco D. V.

Subjects
Physics - Physics Education
Abstract

In view of the difficulties encountered in science teaching, specifically in the teaching of physics, studies aim to develop methodologies that help science teaching. In this context, the present work proposes the use of the Arduino platform as a methodological tool to improve the teaching of physics in high school. For this purpose, the objective of the work was to produce physics experiments with the aid of Arduino that can be used in the classroom and thus we investigated that the experiments provided students with a dynamic and close to ideal class in physics teaching. For the development of the research, two experiments were presented, in the areas of thermology and optics, for students of the second grade of high school from two public schools in the city of Picos (PI). After the development of the experimental activity, a simple questionnaire was applied that aimed to support whether the experiments used with Arduino contributed to the teaching of physics. The results obtained were discussed based on the questionnaire applied, indicating that the experiments produced with Arduino contributed significantly to teaching physics learning, indicating that it is an excellent methodological tool for teaching physics and science in general, Comment: 15 pages, 21 figures, language portuguese

Published
2020

12. Diverse Bacterial Genes Modulate Plant Root Association by Beneficial Bacteria

Author

do Amaral, Fernanda Plucani, Tuleski, Thalita Regina, Pankievicz, Vania Carla Silva, Melnyk, Ryan A, Arkin, Adam P, Griffitts, Joel, Tadra-Sfeir, Michelle Zibetti, de Souza, Emanuel Maltempi, Deutschbauer, Adam, Monteiro, Rose Adele, and Stacey, Gary

Subjects
Microbiology, Biological Sciences, Zero Hunger, Arabidopsis, Azoarcus, Bacterial Proteins, Herbaspirillum, Iron, Plant Roots, Rhizosphere, Setaria Plant, Soil Microbiology, bacterium-root colonization, beneficial bacteria, gene functionality, transposon mutagenesis, Biochemistry and cell biology, Medical microbiology
Abstract

The plant rhizosphere harbors a diverse population of microorganisms, including beneficial plant growth-promoting bacteria (PGPB), that colonize plant roots and enhance growth and productivity. In order to specifically define bacterial traits that contribute to this beneficial interaction, we used high-throughput transposon mutagenesis sequencing (TnSeq) in two model root-bacterium systems associated with Setaria viridis: Azoarcus olearius DQS4T and Herbaspirillum seropedicae SmR1. This approach identified ∼100 significant genes for each bacterium that appeared to confer a competitive advantage for root colonization. Most of the genes identified specifically in A. olearius encoded metabolism functions, whereas genes identified in H. seropedicae were motility related, suggesting that each strain requires unique functions for competitive root colonization. Genes were experimentally validated by site-directed mutagenesis, followed by inoculation of the mutated bacteria onto S. viridis roots individually, as well as in competition with the wild-type strain. The results identify key bacterial functions involved in iron uptake, polyhydroxybutyrate metabolism, and regulation of aromatic metabolism as important for root colonization. The hope is that by improving our understanding of the molecular mechanisms used by PGPB to colonize plants, we can increase the adoption of these bacteria in agriculture to improve the sustainability of modern cropping systems.IMPORTANCE There is growing interest in the use of associative, plant growth-promoting bacteria (PGPB) as biofertilizers to serve as a sustainable alternative for agriculture application. While a variety of mechanisms have been proposed to explain bacterial plant growth promotion, the molecular details of this process remain unclear. The current research supports the idea that PGPB use in agriculture will be promoted by gaining more knowledge as to how these bacteria colonize plants, promote growth, and do so consistently. Specifically, the research seeks to identify those bacterial genes involved in the ability of two, PGPB strains, Azoarcus olearius and Herbaspirillum seropedicae, to colonize the roots of the C4 model grass Setaria viridis. Applying a transposon mutagenesis (TnSeq) approach, we assigned phenotypes and function to genes that affect bacterial competitiveness during root colonization. The results suggest that each bacterial strain requires unique functions for root colonization but also suggests that a few, critical functions are needed by both bacteria, pointing to some common mechanisms. The hope is that such information can be exploited to improve the use and performance of PGPB in agriculture.

Published
2020

13. Shed Light in the DaRk LineagES of the Fungal Tree of Life-STRES.

Author

Selbmann, Laura, Benkő, Zsigmond, Coleine, Claudia, de Hoog, Sybren, Donati, Claudio, Druzhinina, Irina, Emri, Tamás, Ettinger, Cassie L, Gladfelter, Amy S, Gorbushina, Anna A, Grigoriev, Igor V, Grube, Martin, Gunde-Cimerman, Nina, Karányi, Zsolt Ákos, Kocsis, Beatrix, Kubressoian, Tania, Miklós, Ida, Miskei, Márton, Muggia, Lucia, Northen, Trent, Novak-Babič, Monika, Pennacchio, Christa, Pfliegler, Walter P, Pòcsi, Istvàn, Prigione, Valeria, Riquelme, Meritxell, Segata, Nicola, Schumacher, Julia, Shelest, Ekaterina, Sterflinger, Katja, Tesei, Donatella, U'Ren, Jana M, Varese, Giovanna C, Vázquez-Campos, Xabier, Vicente, Vania A, Souza, Emanuel M, Zalar, Polona, Walker, Allison K, and Stajich, Jason E

Subjects
Dothideomycetes, Eurotiomycetes, adaptation, black fungi, extremophiles, genomics, metabolomics, secondary metabolites, stress conditions, transcriptomics
Abstract

The polyphyletic group of black fungi within the Ascomycota (Arthoniomycetes, Dothideomycetes, and Eurotiomycetes) is ubiquitous in natural and anthropogenic habitats. Partly because of their dark, melanin-based pigmentation, black fungi are resistant to stresses including UV- and ionizing-radiation, heat and desiccation, toxic metals, and organic pollutants. Consequently, they are amongst the most stunning extremophiles and poly-extreme-tolerant organisms on Earth. Even though ca. 60 black fungal genomes have been sequenced to date, [mostly in the family Herpotrichiellaceae (Eurotiomycetes)], the class Dothideomycetes that hosts the largest majority of extremophiles has only been sparsely sampled. By sequencing up to 92 species that will become reference genomes, the "Shed light in The daRk lineagES of the fungal tree of life" (STRES) project will cover a broad collection of black fungal diversity spread throughout the Fungal Tree of Life. Interestingly, the STRES project will focus on mostly unsampled genera that display different ecologies and life-styles (e.g., ant- and lichen-associated fungi, rock-inhabiting fungi, etc.). With a resequencing strategy of 10- to 15-fold depth coverage of up to ~550 strains, numerous new reference genomes will be established. To identify metabolites and functional processes, these new genomic resources will be enriched with metabolomics analyses coupled with transcriptomics experiments on selected species under various stress conditions (salinity, dryness, UV radiation, oligotrophy). The data acquired will serve as a reference and foundation for establishing an encyclopedic database for fungal metagenomics as well as the biology, evolution, and ecology of the fungi in extreme environments.

Published
2020

14. BNT162b2 mRNA COVID-19 against symptomatic Omicron infection following a mass vaccination campaign in southern Brazil: A prospective test-negative design study

Author

Rosa, Regis Goulart, Falavigna, Maicon, Manfio, Josélia Larger, de Araujo, Cintia Laura Pereira, Cohen, Mírian, do Valle Barbosa, Gynara Rezende Gonzalez, de Souza, Ana Paula, Romeiro Silva, Fernanda Kelly, Sganzerla, Daniel, da Silva, Mariana Motta Dias, Ferreira, Diogo, de Oliveira Rodrigues, Cristina, de Souza, Emanuel Maltempi, de Oliveira, Jaqueline Carvalho, Gradia, Daniela Fiori, Brandalize, Ana Paula Carneiro, Royer, Carla Adriane, Luiz, Rafael Messias, Kucharski, Gabriela Almeida, Pedrotti, Fernando, Valluri, Srinivas Rao, Srivastava, Amit, Julião, Viviane Wal, Melone, Olga Chameh, Allen, Kristen E., Kyaw, Moe H., Spinardi, Julia, del Carmen Morales Castillo, Graciela, and McLaughlin, John M.

Published
2023
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17. Oral Administration of Lactobacillus acidophilus LA5 Prevents Alveolar Bone Loss and Alters Oral and Gut Microbiomes in a Murine Periodontitis Experimental Model

Author

Cataruci, Amalia C. S., primary, Kawamoto, Dione, additional, Shimabukuro, Natali, additional, Ishikawa, Karin H., additional, Ando-Suguimoto, Ellen S., additional, Ribeiro, Rodolfo A., additional, Nicastro, Gianlucca G., additional, Albuquerque-Souza, Emanuel, additional, de Souza, Robson F., additional, and Mayer, Marcia P. A., additional

Published
2024
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19. Características hidrogeomorfométricas da microbacia Igarapé Branco, Amazônia Ocidental, Brasil

Author

Alves, Keitielen dos Santos Ferreira, primary, Santos Junior, Nilson Reinaldo Fernades dos, additional, Cavalheiro, Wanderson Cleiton Schmidt, additional, Souza, Renato Francisco da Silva, additional, Souza, Emanuel Fernando Maia de, additional, Fulan, João Ânderson, additional, Stachiw, Rosalvo, additional, Santos, Miquele Araújo dos, additional, Ferreira, Elvino, additional, Rodrigues, Antônio Augusto Marques, additional, Nascimento, João Marcelo Silva do, additional, Macedo, Rodrigo Santana, additional, and Vendruscolo, Jhony, additional

Published
2024
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20. Infection of SARS-CoV-2 in domestic dogs associated with owner viral load

Author

de Souza Barbosa, Altina Bruna, Kmetiuk, Louise Bach, de Carvalho, Otávio Valério, Brandão, Ana Pérola Drulla, Doline, Fernando Rodrigo, Lopes, Sibele Rejane Rangel Silveira, Meira, Douglas Adamoski, de Souza, Emanuel Maltempi, da Silva Trindade, Edvaldo, Baura, Valter, Barbosa, David Soeiro, Brandespim, Daniel Friguglietti, Galhardo, Juliana Arena, Pettan-Brewer, Christina, de Sá Guimarães, Ana Marcia, Dutra, Valéria, de Morais, Hélio Autran, Huergo, Luciano, dos Santos, Andrea Pires, and Biondo, Alexander Welker

Published
2022
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22. Large-Scale Screening of Asymptomatic Persons for SARS-CoV-2 Variants of Concern and Gamma Takeover, Brazil

Author

Adamoski, Douglas, de Oliveira, Jaqueline Carvalho, Bonatto, Ana Claudia, Wassem, Roseli, Nogueira, Meri Bordignon, Raboni, Sonia Mara, Trindade, Edvaldo da Silva, de Souza, Emanuel Maltempi, and Gradia, Daniela Fiori

Subjects
Carrier state (Communicable diseases) -- Statistics -- Diagnosis, Health
Abstract

Ongoing screening for active severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections, coupled with contact tracing, can efficiently reduce viral transmission within the community (1). However, as new and more [...]

Published
2021
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23. Antigen production and development of an indirect ELISA based on the nucleocapsid protein to detect human SARS-CoV-2 seroconversion

Author

Conzentino, Marcelo S., Forchhammer, Karl, Souza, Emanuel M., Pedrosa, Fábio O., Nogueira, Meri B., Raboni, Sônia M., Rego, Fabiane G. M., Zanette, Dalila L., Aoki, Mateus N., Nardin, Jeanine M., Fornazari, Bruna, Morales, Hugo M. P., Celedon, Paola A. F., Lima, Carla V. P., Mattar, Sibelle B., Lin, Vanessa H., Morello, Luis G., Marchini, Fabricio K., Reis, Rodrigo A., and Huergo, Luciano F.

Published
2021
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27. VULVOVAGINITES

Author

SOUZA, EMANUEL CORDEIRO, primary, SILVA, LUCAS CARVALHO, additional, and BRAGA, LUCAS GABRIEL COUTO, additional

Published
2022
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44. Análise da cobertura do solo como ferramenta para o planejamento e a gestão ambiental na microbacia do Rio Professora Cleci, Amazônia Ocidental, Brasil

Author

Barros, Afonso Henrique Rodrigues de Oliveira, primary, Simão, Beatriz Aparecida Nunes, additional, Veríssimo, Erleide Paula Santos, additional, Da Frota, Diana Brito, additional, De Souza, Emanuel Fernando Maia, additional, Da Rocha, Karen Janones, additional, Fulan, João Ânderson, additional, Carneiro, Kalline de Almeida Alves, additional, Cavalheiro, Wanderson Cleiton Schmidt, additional, Rodrigues, João Batista Belarmino, additional, Do Nascimento, João Marcelo Silva, additional, and Vendruscolo, Jhony, additional

Published
2023
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45. M\'etodo de BPHZ para Pontos de Lifshitz m-Axiais Anisotr\'opicos

Author

Souza, Emanuel V.

Subjects
Condensed Matter - Other Condensed Matter, Condensed Matter - Statistical Mechanics, High Energy Physics - Theory
Abstract

In this work we investigate the critical behavior of physical systems with competing interactions that present points Lifshitz m-axial. For this study we used the techniques of Quantum Field Theory with Massive Scalar interactions of type {\lambda}{\phi}^4 in order to obtain a perturbative expansion for the two-point vertex part up to the 3-loop order and four-point vertex function up to 2-loop level. These vertex functions were regularized using the method of dimensional regularization and renormalized using the minimal subtraction of dimensional poles method.In particular, counterterms have been added to the original Lagrangian, which is the main feature of the method BPHZ (Bogoliubov-Parasiuk-Hepp-Zimmermann). Through the renormalization group ideas, we defined the Wilson functions which shall produce nontrivial fixed points, and from these functions and fixed points, we calculate the anisotropic critical exponents {\eta}_{\tau}, to the order of three in number loops, and {\nu}_{\tau} to the number of two in order loops, which characterize the critical behavior of the Lifshitz type m-axial. The exponents calculated using this technique are in perfect agreement with results obtained using other methods, thus confirming the known and important hypothesis of universality., Comment: 95 pages, 8 figures, Thesis Master, language Portuguese

Published
2014

46. Massive minimal subtraction scheme and 'partial-$p$' in anisotropic Lifshitz space(time)s

Author

Souza, Emanuel V., Carvalho, Paulo R. S., and Leite, Marcelo M.

Subjects
High Energy Physics - Theory, Condensed Matter - Statistical Mechanics, General Relativity and Quantum Cosmology, High Energy Physics - Phenomenology, Mathematical Physics
Abstract

We introduce the "partial-$p$" operation in a massive Euclidean $\lambda\phi^{4}$ scalar field theory describing anisotropic Lifshitz critical behavior. We then develop a minimal subtraction a la $Bogoliubov-Parasyuk-Hepp-Zimmermann$ renormalization scheme. As an application we compute critical exponents diagrammatically using the orthogonal approximation at least up to two-loop order and show their equivalence with other renormalization techniques. We discuss possible applications of the method in other field-theoretic contexts., Comment: RevTex, 6 pages, 16 figures

Published
2014

49. Re-emergence of Gamma-like-II and emergence of Gamma-S:E661D SARS-CoV-2 lineages in the south of Brazil after the 2021 outbreak

Author

Oliveira, Mauro M., Schemberger, Michelle O., Suzukawa, Andreia A., Riediger, Irina N., do Carmo Debur, Maria, Becker, Guilherme, Resende, Paola Cristina, Gräf, Tiago, Balsanelli, Eduardo, de Baura, Valter Antônio, de Souza, Emanuel M., Pedrosa, Fábio O., Alves, Lysangela R., Blanes, Lucas, Nardelli, Sheila Cristina, Aguiar, Alessandra M., Albrecht, Letusa, Zanette, Dalila, Ávila, Andréa R., Morello, Luis Gustavo, Marchini, Fabricio K., dos Santos, Hellen G., Passetti, Fabio, Dallagiovanna, Bruno, and Faoro, Helisson

Published
2021
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