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12 results on '"Souza, Eduardo V."'

2. Profiling mouse brown and white adipocytes to identify metabolically relevant small ORFs and functional microproteins

Author

Martinez, Thomas F, Lyons-Abbott, Sally, Bookout, Angie L, De Souza, Eduardo V, Donaldson, Cynthia, Vaughan, Joan M, Lau, Calvin, Abramov, Ariel, Baquero, Arian F, Baquero, Karalee, Friedrich, Dave, Huard, Justin, Davis, Ray, Kim, Bong, Koch, Ty, Mercer, Aaron J, Misquith, Ayesha, Murray, Sara A, Perry, Sakara, Pino, Lindsay K, Sanford, Christina, Simon, Alex, Zhang, Yu, Zipp, Garrett, Bizarro, Cristiano V, Shokhirev, Maxim N, Whittle, Andrew J, Searle, Brian C, MacCoss, Michael J, Saghatelian, Alan, and Barnes, Christopher A

Subjects
Genetics, Diabetes, Nutrition, Obesity, Metabolic and endocrine, Humans, Animals, Mice, Adipocytes, White, Adipose Tissue, Brown, Open Reading Frames, Adipose Tissue, White, Adipocytes, Brown, Ribo-seq, brown adipose tissue, data-independent acquisition mass spectrometry, diet-induced obesity, microproteins, ribosome profiling, secreted microproteins, small ORFs, white adipose tissue, Biochemistry and Cell Biology, Medical Biochemistry and Metabolomics, Endocrinology & Metabolism
Abstract

Microproteins (MPs) are a potentially rich source of uncharacterized metabolic regulators. Here, we use ribosome profiling (Ribo-seq) to curate 3,877 unannotated MP-encoding small ORFs (smORFs) in primary brown, white, and beige mouse adipocytes. Of these, we validated 85 MPs by proteomics, including 33 circulating MPs in mouse plasma. Analyses of MP-encoding mRNAs under different physiological conditions (high-fat diet) revealed that numerous MPs are regulated in adipose tissue in vivo and are co-expressed with established metabolic genes. Furthermore, Ribo-seq provided evidence for the translation of Gm8773, which encodes a secreted MP that is homologous to human and chicken FAM237B. Gm8773 is highly expressed in the arcuate nucleus of the hypothalamus, and intracerebroventricular administration of recombinant mFAM237B showed orexigenic activity in obese mice. Together, these data highlight the value of this adipocyte MP database in identifying MPs with roles in fundamental metabolic and physiological processes such as feeding.

Published
2023

6. Handling the Hurdles on the Way to Anti-tuberculosis Drug Development

Author

Dalberto, Pedro F., primary, de Souza, Eduardo V., additional, Abbadi, Bruno L., additional, Neves, Christiano E., additional, Rambo, Raoní S., additional, Ramos, Alessandro S., additional, Macchi, Fernanda S., additional, Machado, Pablo, additional, Bizarro, Cristiano V., additional, and Basso, Luiz A., additional

Published
2020
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11. The symmetrical differential Buck–Boost DC‐DC converter with partial power processing applied to photovoltaic systems.

Author

Schmitz, Lenon, Gonçalves, Christopher F., Schlingmann, André, Souza, Eduardo V., Eyng, Juliana, Martins, Denizar C., and Coelho, Roberto F.

Subjects
*MAXIMUM power point trackers, *DC-to-DC converters, *PHOTOVOLTAIC power systems, *COMPUTER performance
Abstract

Summary This paper presents the symmetric differential Buck–Boost converter, obtained from the differential connection between two Buck–Boost converters. The topology is characterized by presenting low‐voltage ripple, partial power processing, ability to provide intermediate voltage gains (1 < M < 10) and design simplicity. In this configuration, the output voltage is composed of the sum of the input voltage and the output voltages of both converters differentially connected. The topology description along with its main equations and waveforms in continuous conduction mode with phase‐shift modulation are presented in this paper. Moreover, a mathematical analysis that demonstrates its capability of performing with partial power processing and a comparative analysis with similar topologies are also presented. In addition, experimental results extracted from a prototype are presented and discussed. Because the proposed converter is applied to photovoltaic energy processing, it is validated in open loop and as maximum power point tracker, in which the obtained CEC efficiency is 97.8%. [ABSTRACT FROM AUTHOR]

Published
2024
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12. The Integration of Proteogenomics and Ribosome Profiling Circumvents Key Limitations to Increase the Coverage and Confidence of Novel Microproteins.

Author

de Souza EV, Bookout AL, Barnes CA, Miller B, Machado P, Basso LA, Bizarro CV, and Saghatelian A

Abstract

There has been a dramatic increase in the identification of non-conical translation and a significant expansion of the protein-coding genome and proteome. Among the strategies used to identify novel small ORFs (smORFs), Ribosome profiling (Ribo-Seq) is the gold standard for the annotation of novel coding sequences by reporting on smORF translation. In Ribo-Seq, ribosome-protected footprints (RPFs) that map to multiple sites in the genome are computationally removed since they cannot unambiguously be assigned to a specific genomic location, or to a specific transcript in the case of multiple isoforms. Furthermore, RPFs necessarily result in short (25-34 nucleotides) reads, increasing the chance of ambiguous and multi-mapping alignments, such that smORFs that reside in these regions cannot be identified by Ribo-Seq. Here, we show that the inclusion of proteogenomics to create a Ribosome Profiling and Proteogenomics Pipeline (RP3) bypasses this limitation to identify a group of microprotein-encoding smORFs that are missed by current Ribo-Seq pipelines. Moreover, we show that the microproteins identified by RP3 have different sequence compositions from the ones identified by Ribo-Seq-only pipelines, which can affect proteomics identification. In aggregate, the development of RP3 maximizes the detection and confidence of protein-encoding smORFs and microproteins.

Published
2023
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