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1. Exacerbation Burden in COPD and Occurrence of Mortality in a Cohort of Italian Patients: Results of the Gulp Study

Author

Santus P, Di Marco F, Braido F, Contoli M, Corsico AG, Micheletto C, Pelaia G, Radovanovic D, Rogliani P, Saderi L, Scichilone N, Tanzi S, Vella M, Boarino S, Sotgiu G, and Solidoro P

Subjects
pulmonary disease chronic obstructive, heart failure, ischaemic heart disease, respiratory medicine, public health, Diseases of the respiratory system, RC705-779
Abstract

Pierachille Santus,1 Fabiano Di Marco,2 Fulvio Braido,3 Marco Contoli,4 Angelo Guido Corsico,5 Claudio Micheletto,6 Girolamo Pelaia,7 Dejan Radovanovic,1 Paola Rogliani,8 Laura Saderi,9 Nicola Scichilone,10 Silvia Tanzi,11 Manlio Vella,11 Silvia Boarino,11 Giovanni Sotgiu,9 Paolo Solidoro12 1Department of Biomedical and Clinical Sciences (DIBIC), Università degli Studi di Milano, Division of Respiratory Diseases, Ospedale L. Sacco, ASST Fatebenefratelli-Sacco, Milano, Italy; 2Department of Health Sciences, Università degli Studi di Milano Pneumology, ASST Papa Giovanni XXIII, Bergamo, Italy; 3Department of Internal Medicine (DiMI), Respiratory Unit for Continuity of Care, IRCCS Ospedale Policlinico San Martino, University of Genova, Genova, Italy; 4Department of Translational Medicine, Respiratory Section, University of Ferrara, Ferrara, Italy; 5Department of Medical Sciences and Infective Diseases, Unit of Respiratory Diseases, IRCCS Policlinico San Matteo Foundation and University of Pavia Medical School, Pavia, Italy; 6Cardio-Thoracic Department, Respiratory Unit, University Integrated Hospital, Verona, Italy; 7Dipartimento di Scienze della Salute, Università Magna Graecia, Catanzaro, Italy; 8Department of Experimental Medicine, Unit of Respiratory Medicine, University of Rome ”Tor Vergata”, Division of Respiratory Medicine, University Hospital ”Tor Vergata”, Rome, Italy; 9Department of Medicine, Surgery and Pharmacy, University of Sassari, Sassari, Italy; 10Biomedical Department of Internal and Specialist Medicine, University of Palermo, Palermo, Italy; 11AstraZeneca Italia, Milan, Italy; 12Department of Medical Sciences, University of Turin, S.C. Pneumologia, Azienda Ospedaliero Universitaria Città della Salute e della Scienza, Torino, ItalyCorrespondence: Pierachille Santus, Università degli Studi di Milano, Via G.B. Grassi 74, Milano, 20157, Italy, Tel +39 0239042801, Fax +39 0239042473, Email pierachille.santus@unimi.itObjective: To describe the burden of moderate to severe exacerbations and all-cause mortality; the secondary objectives were to analyze treatment patterns and changes over follow-up.Design: Observational, multicenter, retrospective, cohort study with a three year follow-up period.Setting: Ten Italian academic secondary- and tertiary-care centers.Participants: Patients with a confirmed diagnosis of COPD referring to the outpatient clinics of the participating centers were retrospectively recruited.Primary and Secondary Outcome Measures: Annualized frequency of moderate and severe exacerbations stratified by exacerbation history prior to study enrollment. Patients were classified according to airflow obstruction, GOLD risk categories, and divided in 4 groups: A = no exacerbations; B = 1 moderate exacerbation; C = 1 severe exacerbation; D = ≥ 2 moderate and/or severe exacerbations. Overall all-cause mortality stratified by age, COPD category, and COPD therapy. A logistic regression model assessed the association of clinical characteristics with mortality.Results: 1111 patients were included (73% males), of which 41.5% had a history of exacerbations. As expected, the proportion of patients experiencing ≥ 1 exacerbation during follow-up increased according to pre-defined study risk categories (B: 79%, C: 84%, D: 97.4%). Overall, by the end of follow-up, 45.5% of patients without a history of exacerbation experienced an exacerbation (31% of which severe), and 13% died. Deceased patients were significantly older, more obstructed and hyperinflated, and more frequently active smokers compared with survivors. Severe exacerbations were more frequent in patients that died (23.5%, vs 10.2%; p-value: 0.002). Chronic heart failure and ischemic heart disease were the only comorbidities associated with a higher odds ratio (OR) for death (OR: 2.2, p-value: 0.001; and OR: 1.9, p-value: 0.007). Treatment patterns were similar in patients that died and survivors.Conclusion: Patients with a low exacerbation risk are exposed to a significant future risk of moderate/severe exacerbations. Real life data confirm the strong association between mortality and cardiovascular comorbidities in COPD.Keywords: pulmonary disease chronic obstructive, heart failure, ischaemic heart disease, respiratory medicine, public health

Published
2024

3. Infections in lung transplanted patients: A review

Author

Dettori, M., Riccardi, N., Canetti, D., Antonello, R.M., Piana, A.F., Palmieri, A., Castiglia, P., Azara, A.A., Masia, M.D., Porcu, A., Ginesu, G.C., Cossu, M.L., Conti, M., Pirina, P., Fois, A., Maida, I., Madeddu, G., Babudieri, S., Saderi, L., and Sotgiu, G.

Published
2024
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4. Clinical standards for the assessment, management and rehabilitation of post-TB lung disease

Author

Migliori, GB, Marx, FM, Ambrosino, N, Zampogna, E, Schaaf, HS, van der Zalm, MM, Allwood, B, Byrne, AL, Mortimer, K, Wallis, RS, Fox, GJ, Leung, CC, Chakaya, JM, Seaworth, B, Rachow, A, Marais, BJ, Furin, J, Akkerman, OW, Al Yaquobi, F, Amaral, AFS, Borisov, S, Caminero, JA, Carvalho, ACC, Chesov, D, Codecasa, LR, Teixeira, RC, Dalcolmo, MP, Datta, S, Dinh-Xuan, A-T, Duarte, R, Evans, CA, García-García, J-M, Günther, G, Hoddinott, G, Huddart, S, Ivanova, O, Laniado-Laborín, R, Manga, S, Manika, K, Mariandyshev, A, Mello, FCQ, Mpagama, SG, Muñoz-Torrico, M, Nahid, P, Ong, CWM, Palmero, DJ, Piubello, A, Pontali, E, Silva, DR, Singla, R, Spanevello, A, Tiberi, S, Udwadia, ZF, Vitacca, M, Centis, R, D Ambrosio, L, Sotgiu, G, Lange, C, and Visca, D

Subjects
Lung, Clinical Research, 7.3 Management and decision making, Management of diseases and conditions, Good Health and Well Being, Humans, Consensus, Lung Diseases, Quality of Life, Tuberculosis, tuberculosis, post-TB lung disease, se-quelae, pulmonary rehabilitation, clinical standards, Cardiorespiratory Medicine and Haematology, Microbiology
Abstract

BACKGROUND: Increasing evidence suggests that post-TB lung disease (PTLD) causes significant morbidity and mortality. The aim of these clinical standards is to provide guidance on the assessment and management of PTLD and the implementation of pulmonary rehabilitation (PR).METHODS: A panel of global experts in the field of TB care and PR was identified; 62 participated in a Delphi process. A 5-point Likert scale was used to score the initial ideas for standards and after several rounds of revision the document was approved (with 100% agreement).RESULTS: Five clinical standards were defined: Standard 1, to assess patients at the end of TB treatment for PTLD (with adaptation for children and specific settings/situations); Standard 2, to identify patients with PTLD for PR; Standard 3, tailoring the PR programme to patient needs and the local setting; Standard 4, to evaluate the effectiveness of PR; and Standard 5, to conduct education and counselling. Standard 6 addresses public health aspects of PTLD and outcomes due to PR.CONCLUSION: This is the first consensus-based set of Clinical Standards for PTLD. Our aim is to improve patient care and quality of life by guiding clinicians, programme managers and public health officers in planning and implementing adequate measures to assess and manage PTLD.

Published
2021

9. Alpha-1 deficiency in severe asthma patients

Author

Zappa, M., primary, Grossi, S., additional, Pignatti, P., additional, Pini, L., additional, Centis, R., additional, Migliori, G.B., additional, Ardesi, F., additional, Sotgiu, G., additional, Corsico, A. G., additional, Spanevello, A., additional, and Visca, D., additional

Published
2024
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11. Outcome of treatment of MDR-TB or drug-resistant patients treated with bedaquiline and delamanid: Results from a large global cohort

Author

Koirala, S., Borisov, S., Danila, E., Mariandyshev, A., Shrestha, B., Lukhele, N., Dalcolmo, M., Shakya, S.R., Miliauskas, S., Kuksa, L., Manga, S., Aleksa, A., Denholm, J.T., Khadka, H.B., Skrahina, A., Diktanas, S., Ferrarese, M., Bruchfeld, J., Koleva, A., Piubello, A., Koirala, G.S., Udwadia, Z.F., Palmero, D.J., Munoz-Torrico, M., GC, R., Gualano, G., Grecu, V.I., Motta, I., Papavasileiou, A., Li, Y., Hoefsloot, W., Kunst, H., Mazza-Stalder, J., Payen, M.-C., Akkerman, O.W., Bernal, E., Manfrin, V., Matteelli, A., Mustafa Hamdan, H., Nieto Marcos, M., Cadiñanos Loidi, J., Cebrian Gallardo, J.J., Duarte, R., Escobar Salinas, N., Gomez Rosso, R., Laniado-Laborín, R., Martínez Robles, E., Quirós Fernandez, S., Rendon, A., Solovic, I., Tadolini, M., Viggiani, P., Belilovski, E., Boeree, M.J., Cai, Q., Davidavičienė, E., Forsman, L.D., De Los Rios, J., Drakšienė, J., Duga, A., Elamin, S.E., Filippov, A., Garcia, A., Gaudiesiute, I., Gavazova, B., Gayoso, R., Gruslys, V., Jonsson, J., Khimova, E., Madonsela, G., Magis-Escurra, C., Marchese, V., Matei, M., Moschos, C., Nakčerienė, B., Nicod, L., Palmieri, F., Pontarelli, A., Šmite, A., Souleymane, M.B., Vescovo, M., Zablockis, R., Zhurkin, D., Alffenaar, J.-W., Caminero, J.A., Codecasa, L.R., García-García, J.-M., Esposito, S., Saderi, L., Spanevello, A., Visca, D., Tiberi, S., Pontali, E., Centis, R., D'Ambrosio, L., van den Boom, M., Sotgiu, G., and Migliori, G.B.

Published
2021
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15. Diagnostic Accuracy of DNA-Methylation in Detection of Cervical Dysplasia: Findings from a Population-Based Screening Program

Author

Muresu, N, Puci, M, Sotgiu, G, Sechi, I, Usai, M, Cossu, A, Martinelli, M, Cocuzza, C, Piana, A, Muresu, Narcisa, Puci, Mariangela V., Sotgiu, Giovanni, Sechi, Illari, Usai, Manuela, Cossu, Andrea, Martinelli, Marianna, Cocuzza, Clementina Elvezia, Piana, Andrea, Muresu, N, Puci, M, Sotgiu, G, Sechi, I, Usai, M, Cossu, A, Martinelli, M, Cocuzza, C, Piana, A, Muresu, Narcisa, Puci, Mariangela V., Sotgiu, Giovanni, Sechi, Illari, Usai, Manuela, Cossu, Andrea, Martinelli, Marianna, Cocuzza, Clementina Elvezia, and Piana, Andrea

Abstract

Background: Epigenetic biomarkers in cancer have emerged as promising tools for early detection, prognosis, and treatment response prediction. In cervical cells, hypermethylation of the host and viral HPV-genome increases with the severity of lesions, providing a useful biomarker in the triage of hr-HPV-positive women and during treatment. The present study focuses on evaluating the clinical performance of the FAM19A4/miR124-2 methylation test in a population-based cervical screening program. Methods: Previously collected cervical samples, after bisulfite-converted DNA, were analyzed by PreCursor-M+ kit (distributed by Fujirebio Europe), for DNA methylation. The sensitivity, specificity, and negative/positive predictive values of DNA methylation were compared to histology, colposcopy, the HPV-DNA test, and cytology results. Results: Among the 61-sample set, the specificity of methylation vs. positive histology (≥CIN2) and colposcopy (≥G2) were 87% and 90%, whereas the sensitivity was 50% and 33.3%, respectively. The combination of methylation analysis with standard methods increases diagnostic accuracy. Conclusions: Overall, we found a good specificity of DNA methylation in comparison to currently used techniques. Further larger studies could support the use of FAM19A4/miR124-2 as reliable biomarkers in the prevention of cervical cancer as triage in the screening protocol.

Published
2024

16. Diagnostic delay in bronchiectasis: an Italian perspective

Author

Chessarí, C, Simonetta, E, Amati, F, Nigro, M, Stainer, A, Sotgiu, G, Puci, M, Gramegna, A, Blasi, F, Morlacchi, L, Buscemi, A, Conio, V, Sanci, V, Corsico, A, Faverio, P, Michalak, W, Luppi, F, Crimi, C, Vancheri, C, Campisi, R, Vulpi, M, Carpagnano, G, Cicchetti, M, Sekretna, K, Scichilone, N, Battaglia, S, Aliberti, S, Morlacchi, LC, Buscemi, AAMD, Corsico, AG, Vulpi, MR, Carpagnano, GE, Chessarí, C, Simonetta, E, Amati, F, Nigro, M, Stainer, A, Sotgiu, G, Puci, M, Gramegna, A, Blasi, F, Morlacchi, L, Buscemi, A, Conio, V, Sanci, V, Corsico, A, Faverio, P, Michalak, W, Luppi, F, Crimi, C, Vancheri, C, Campisi, R, Vulpi, M, Carpagnano, G, Cicchetti, M, Sekretna, K, Scichilone, N, Battaglia, S, Aliberti, S, Morlacchi, LC, Buscemi, AAMD, Corsico, AG, Vulpi, MR, and Carpagnano, GE

Abstract

It takes ∼3.5 years to reach a diagnosis of bronchiectasis from onset of symptoms: the long patient's journey in Italy https://bit.ly/46XMWAz.

Published
2024

17. Evaluating Cefiderocol in the Treatment of Multidrug-Resistant Gram-Negative Bacilli: A Review of the Emerging Data

Author

Giacobbe DR, Ciacco E, Girmenia C, Pea F, Rossolini GM, Sotgiu G, Tascini C, Tumbarello M, Viale P, and Bassetti M

Subjects
cefiderocol, siderophore, pseudomonas, acinetobacter, enterobacterales, antimicrobial resistance, Infectious and parasitic diseases, RC109-216
Abstract

Daniele Roberto Giacobbe,1,2 Eugenio Ciacco,3 Corrado Girmenia,4 Federico Pea,5,6 Gian Maria Rossolini,7,8 Giovanni Sotgiu,9 Carlo Tascini,10 Mario Tumbarello,11,12 Pierluigi Viale,5,6 Matteo Bassetti1,2 On behalf of the ISGRI-SITA (Italian Study Group on Resistant Infections of the Italian Society of Anti-infective Therapy)1Clinica Malattie Infettive, Ospedale Policlinico San Martino – IRCCS, Genoa, Italy; 2Department of Health Sciences, University of Genoa, Genoa, Italy; 3Pharmacy Unit, S. Salvatore Hospital, ASL1 Abruzzo, L’Aquila, Italy; 4Hematology, Dipartimento Medicina Traslazionale e di Precisione, AOU Policlinico Umberto I, Sapienza University of Rome, Rome, Italy; 5Department of Medical and Surgical Sciences, Alma Mater Studiorum, University of Bologna, Bologna, Italy; 6University Hospital IRCCS Policlinico Sant’Orsola Bologna, Bologna, Italy; 7Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy; 8Clinical Microbiology and Virology Unit, Florence Careggi University Hospital, Florence, Italy; 9Department of Medical, Surgical and Experimental Sciences, University of Sassari, Sassari, Italy; 10SOC Malattie Infettive, Azienda Sanitaria Integrata, University of Udine, Udine, Italy; 11Dipartimento di Scienze di Laboratorio e Infettivologiche, Fondazione Policlinico Universitario A. Gemelli IRCCS, Rome, Italy; 12Dipartimento di Sicurezza e Bioetica, Università Cattolica del Sacro Cuore, Rome, ItalyCorrespondence: Daniele Roberto GiacobbeClinica Malattie Infettive, Ospedale Policlinico San Martino – IRCCS, L.go R. Benzi 10, Genoa 16132, ItalyTel +390105554658Email daniele.roberto.giacobbe@gmail.comAbstract: Infections due to multidrug-resistant Gram-negative bacteria (MDR-GNB), especially when carbapenem resistant, have been very difficult to manage in the last fifteen years, owing to the paucity of dependable therapeutic options. Cefiderocol is a siderophore cephalosporin recently approved by the Food and Drug Administration (FDA) and European Medicines Agency (EMA) that may have the potential to fill some of the remaining gaps in the treatment of MDR-GNB infections. Among others, cefiderocol demonstrated in vitro activity against carbapenem-resistant Acinetobacter baumannii and metallo-β-lactamases producers. Clinical data from both registrative studies and post-marketing experiences are essential to confirm whether these promises from in vitro studies could readily translate into clinical practice, as well as to delineate the precise place in therapy for cefiderocol for the treatment of MDR-GNB in the near future. Because of its unique potential, it is essential to provide both randomized controlled trials (RCT) and real-life data to improve the ability of clinicians to exploit its benefit in both empirical and targeted treatment of MDR-GNB infections. In this narrative review, we discuss the emerging data from pivotal RCT and initial real-life experiences on the use of cefiderocol for the treatment of MDR-GNB infections.Keywords: cefiderocol, siderophore, Pseudomonas, Acinetobacter, Enterobacterales, antimicrobial resistance

Published
2020

20. Advantages and Challenges of Tailored Regimens for Drug-Resistant Tuberculosis: A StopTB Italia Look into the Future

Author

Riccardi N, Villa S, Alagna R, Giacomelli A, Saderi L, Cirillo DM, Besozzi G, Sotgiu G, and Codecasa L

Subjects
tuberculosis, drug-resistance, treatment, tailored, stoptb., Infectious and parasitic diseases, RC109-216
Abstract

Niccolò Riccardi,1,2,* Simone Villa,1,3,* Riccardo Alagna,1,4 Andrea Giacomelli,1,5 Laura Saderi,1,6 Daniela Maria Cirillo,1,4 Giorgio Besozzi,1 Giovanni Sotgiu,1,6 Luigi Codecasa1,7 1StopTB Italia Onlus, Milan 20159, Italy; 2Department of Infectious - Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital, Negrar di Valpolicella, Verona 37024, Italy; 3Centre for Multidisciplinary Research in Health Science, University of Milan, Milan 20122, Italy; 4IRCCS San Raffaele Scientific Institute, Milan 20132, Italy; 5III Infectious Diseases Unit, ASST Fatebenefratelli Sacco, Milan 20157, Italy; 6Clinical Epidemiology and Medical Statistics Unit, Department of Medical, Surgical and Experimental Sciences, University of Sassari, Sassari 07100, Italy; 7Regional TB Reference Centre, Istituto Villa Marelli, Niguarda Hospital, Milan 20159, Italy*These authors contributed equally to this workCorrespondence: Niccolò RiccardiDepartment of Infectious - Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital, Via Don A. Sempreboni N.5, 37024, Negrar di Valpolicella, Verona, ItalyTel +39 045 601 6420Email niccolo.riccardi@yahoo.itAbstract: The emerge of drug-resistant tuberculosis (TB) strain in recent decades is hampering the efforts of the international community to eliminate the disease worldwide. The World Health Organization (WHO) has drafted many strategies to achieve this ambitious goal. In the very beginning, the aim was to standardize inadequate regimens used in many countries and, thereafter, evolved to tackle the social determinants which hinder TB elimination. However, following the path of narrowing the clinical vision to deal with TB, there is an increased need to personalize the treatment considering both patients and pathogen unique characteristics. In our narrative review, we report the advantages and the backwards in developing a method to implement the concept of precision medicine to the treatment of TB. In this dissertation, we highlight the importance to address different aspects of the diseases encompassing the host and pathogen features, as well as the needs to further implement an adequate follow-up based on the available resources. Nevertheless, many things may hamper the vision of precision medicine in TB, such as the complexity and the costs to develop novel compounds and the costs related to global-scale implementation of patient-centered follow-up. To achieve the ambitious goal of TB elimination, a radical change in TB treatment is needed in order to give a more comprehensive approach based both on patients’ peculiarities and driven by drug susceptibility tests and whole-genome sequencing.Keywords: tuberculosis, drug-resistance, treatment, tailored, StopTB

Published
2020

21. Cancer Pain Management: An Italian Delphi Survey from the Rational Use of Analgesics (RUA) Group

Author

Varrassi G, De Conno F, Orsi L, Puntillo F, Sotgiu G, Zeppetella J, and Zucco F

Subjects
cancer, pain, breakthrough cancer pain, neuropathic pain, delphi survey, Medicine (General), R5-920
Abstract

Giustino Varrassi,1 Franco De Conno,2 Luciano Orsi,3 Filomena Puntillo,4 Giovanni Sotgiu,5 John Zeppetella,6 Furio Zucco7 1Paolo Procacci Foundation, Rome, Italy; 2Palliative Care, Pain Therapy and Rehabilitation Unit, Fondazione IRCCS Istituto Nazionale Tumori, Milan, Italy; 3SC Cure Palliative ASST Carlo Poma, Mantova, Italy; 4Anesthesia and Intensive Care Unit, Department of Emergency and Organ Transplants, University Hospital of Bari, Bari, Italy; 5Clinical Epidemiology and Medical Statistics Unit, Department of Medical, Surgical and Experimental Sciences, University of Sassari, Sassari, Italy; 6St Claire’s Hospice, Hastingwood, UK; 7Azienda Ospedaliera Salvini, Garbagnate Milanese, ItalyCorrespondence: Giustino VarrassiPaolo Procacci Foundation, Via Tacito 7, Rome 00193, ItalyEmail giuvarr@gmail.comBackground: In patients with cancer, the prevalence of pain is high, and pain management is often challenging despite the wide availability of drugs and guidelines.Methods: This Delphi survey was organized within the Rational Use of Analgesics (RUA) Group projects to reach a consensus among Italian palliative care specialists on pain assessment and management. Items were identified from recent publications on cancer pain and guidelines.Results: This survey included input from 190 palliative care specialists representing all Italian territory. A consensus was reached on 17 statements. Items concerning pain assessment achieved over 70% agreement amongst the participants. Items on principles of pain management and management according to type of pain, including breakthrough cancer-related pain and neuropathic pain also achieved high levels of agreement.Conclusion: Results from the RUA project showed that Italian palliative care specialists had a particular interest in items related to pain challenges, in addition to the evaluation and control of pain associated with cancer. However, some discrepancies between current guidelines and clinical practice were observed.Keywords: cancer, pain, breakthrough cancer pain, neuropathic pain, Delphi survey

Published
2020

24. Anticancer activity of paclitaxel-loaded keratin nanoparticles in two-dimensional and perfused three-dimensional breast cancer models

Author

Foglietta F, Spagnoli GC, Muraro MG, Ballestri M, Guerrini A, Ferroni C, Aluigi A, Sotgiu G, and Varchi G

Subjects
keratin, nanoparticles, paclitaxel, breast cancer, perfused three dimensional models, Medicine (General), R5-920
Abstract

Federica Foglietta,1 Giulio C Spagnoli,2,3 Manuele Giuseppe Muraro,2 Marco Ballestri,4 Andrea Guerrini,4 Claudia Ferroni,4 Annalisa Aluigi,4 Giovanna Sotgiu,4 Greta Varchi4 1Department of Drug Science & Technology, University of Torino, Torino, Italy; 2Department of Biomedicine, University of Basel and University Hospital of Basel, Basel, Switzerland; 3Institute of Translational Pharmacology, CNR, Rome, Italy; 4Institute for Organic Synthesis & Photoreactivity, National Research Council, Bologna, Italy Purpose: Taxanes are highly effective cytotoxic drugs for progressing breast cancer treatment. However, their poor solubility and high toxicity urge the development of innovative formulations of potential clinical relevance. Materials and methods: By using a simple and straightforward aggregation method, we have generated paclitaxel (PTX) loaded in keratin nanoparticles (KER-NPs-PTX). Their activities were tested against human breast cancer MCF-7 and MDA MB 231 cell lines in conventional two-dimensional (2D) cultures and in a dynamic three-dimensional (3D) model with perfused bioreactor (p3D). Moreover, KER-NPs-PTX activity was compared to free PTX and to PTX loaded in albumin nanoparticles (HSA-NPs-PTX). Cell viability, induction of apoptosis, and gene expression analysis were used as readouts. Results: In 2D cultures, KER-NPs-PTX was able to inhibit tumor cell viability and to induce apoptosis similarly to PTX and HSA-NPs-PTX. In the p3D model, a lower sensitivity of tumor cells to treatments was observed. Importantly, only KER-NPs-PTX was able to induce a statistically significant increase in apoptotic cell percentages following 24 h treatment for MCF-7 (16.7±4.0 early and 11.3±4.9 late apoptotic cells) and 48 h treatment for MDA MB 231 (21.3±11.2 early and 10.5±1.8 late apoptotic cells) cells. These effects were supported, at least for MCF-7 cells, by significant increases in the expression of proapoptotic BAX gene (5.8±0.5) 24 h after treatment and of cleaved caspase 3 (CC3) protein. Conclusion: KER-NPs-PTX, generated by a simple procedure, is characterized by high water solubility and enhanced PTX-loading ability, as compared to HSA-NPs-PTX. Most importantly, it appears to be able to exert effective anticancer activities on breast cancer cells cultured in 2D or in p3D models. Keywords: keratin, nanoparticles, paclitaxel, breast cancer, perfused three-dimensional models

Published
2018

26. High-Risk Clone of Klebsiella pneumoniae Co-Harbouring Class A and D Carbapenemases in Italy

Author

Del Rio, A, Muresu, N, Sotgiu, G, Saderi, L, Sechi, I, Cossu, A, Usai, M, Palmieri, A, Are, B, Deiana, G, Cocuzza, C, Martinelli, M, Calaresu, E, Piana, A, Del Rio A., Muresu N., Sotgiu G., Saderi L., Sechi I., Cossu A., Usai M., Palmieri A., Are B. M., Deiana G., Cocuzza C., Martinelli M., Calaresu E., Piana A. F., Del Rio, A, Muresu, N, Sotgiu, G, Saderi, L, Sechi, I, Cossu, A, Usai, M, Palmieri, A, Are, B, Deiana, G, Cocuzza, C, Martinelli, M, Calaresu, E, Piana, A, Del Rio A., Muresu N., Sotgiu G., Saderi L., Sechi I., Cossu A., Usai M., Palmieri A., Are B. M., Deiana G., Cocuzza C., Martinelli M., Calaresu E., and Piana A. F.

Abstract

Background: Carbapenem-resistant Klebsiella pneumoniae (CR-Kp) is endemic globally, causing severe infections in hospitalized patients. Surveillance programs help monitor and promptly identify the emergence of new clones. We reported the rapid spread of a novel clone of K. pneumoniae co-harbouring class A and D carbapenemases in colonized patients, and the potential risk factors involved in the development of infections. Methods: Rectal swabs were used for microbiological analyses and detection of the most common carbapenemase encoding genes by real-time PCR (i.e., blaKPC, blaOXA-48, blaNDM, blaVIM, and blaIMP). All strains co-harbouring KPC and OXA-48 genes were evaluated. For each patient, the following variables were collected: age, sex, length and ward of stay, device use, and outcome. Clonality of CR-Kp was assessed by preliminary pulsed field gel electrophoresis (PFGE), followed by multi-locus sequence typing (MLST) analyses. Results: A total of 127 isolates of K. pneumoniae co-harbouring KPC and OXA-48 were collected between September 2019 and December 2020. The median age (IQR) of patients was 70 (61–77). More than 40% of patients were admitted to intensive care unit (ICU). Around 25% of patients developed an invasive infection, the majority of which were respiratory tract infections (17/31; 54.8%). ICU stay and invasive infection increased the risk of mortality (OR: 5.39, 95% CI: 2.42–12.00; OR 6.12, 95% CI: 2.55–14.69, respectively; p-value ≤ 0.001). The antibiotic susceptibility test showed a resistance profile for almost all antibiotics considered. Monoclonal origin was confirmed by PFGE and MLST showing a similar restriction pattern and belonging to ST-512. Conclusions: We report the spread and the marked antibiotic resistance profiles of K. pneumoniae strains co-producing KPC and OXA-48. Further study could clarify the roles of clinical and microbiological variables in the development of invasive infection and increasing risk of mortality, in colonized

Published
2022

27. A systematic review of the costs of diagnosis for multidrug-resistant/extensively drug-resistant TB in different settings

Author

Saderi, L., primary, Cabibbe, A. M., additional, Puci, M., additional, Di Lorenzo, B., additional, Centis, R., additional, Pontali, E., additional, van den Boom, M., additional, Chakaya, J. M., additional, D´Ambrosio, L., additional, Denholm, J. T., additional, Ferrara, G., additional, Silva, D. R., additional, Solovic, I., additional, Spanevello, A., additional, Visca, D., additional, Sotgiu, G., additional, and Migliori, G. B., additional

Published
2023
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28. A systematic review of the costs of diagnosis for multidrug-resistant/extensively drug-resistant TB in different settings.

Author

Saderi, L, Cabibbe, AM, Puci, M, Di Lorenzo, B, Centis, R, Pontali, E, van den Boom, M, Chakaya, JM, D Ambrosio, L, Denholm, JT, Ferrara, G, Silva, DR, Solovic, I, Spanevello, A, Visca, D, Sotgiu, G, Migliori, GB, Saderi, L, Cabibbe, AM, Puci, M, Di Lorenzo, B, Centis, R, Pontali, E, van den Boom, M, Chakaya, JM, D Ambrosio, L, Denholm, JT, Ferrara, G, Silva, DR, Solovic, I, Spanevello, A, Visca, D, Sotgiu, G, and Migliori, GB

Abstract

BACKGROUND: We performed an analysis of the cost and relative merits of different strategies for the diagnosis of multidrug-resistant/extensively drug-resistant TB (MDR/XDR-TB) in different settings.METHODS: We systematically reviewed the published evidence on cost/cost-effectiveness of rapid MDR/pre-XDR-TB and other methods for XDR-TB testing up to September 2022. PRISMA guidelines were followed. Collected data were analysed using Stata v17 software. Cost data were reported in USD ($) and summarised by mean, standard deviation, and range. Country income level was defined according to the World Bank country classification. Three simplified scenarios were also used to explore testing implications, based on low, intermediate and high TB incidence.RESULTS: Of 157 records, 25 studies were included with 24 reporting the cost of Xpert/RIF and two that evaluated the implementation of the MTBDRplus test. The total rapid test cost ranged from $12.41-$218, including $1.13-$74.60 for reagents/consumables and $0.40-$14.34 for equipment.CONCLUSION: The cost of MDR/XDR-TB diagnostics is lower in low resource settings. However, the cost-effective implementation of MDR/XDR-TB diagnostic algorithms requires careful consideration of local resources to avoid missed identification and the use of inappropriate regimen.

Published
2023

29. Preliminary Results of Feasibility and Acceptability of Self-Collection for Cervical Screening in Italian Women

Author

Sechi, I, Muresu, N, Puci, M, Saderi, L, Del Rio, A, Cossu, A, Muroni, M, Castriciano, S, Martinelli, M, Cocuzza, C, Sotgiu, G, Piana, A, Sechi, Illari, Muresu, Narcisa, Puci, Mariangela V., Saderi, Laura, Del Rio, Arcadia, Cossu, Andrea, Muroni, Maria R., Castriciano, Santina, Martinelli, Marianna, Cocuzza, Clementina E., Sotgiu, Giovanni, Piana, Andrea, Sechi, I, Muresu, N, Puci, M, Saderi, L, Del Rio, A, Cossu, A, Muroni, M, Castriciano, S, Martinelli, M, Cocuzza, C, Sotgiu, G, Piana, A, Sechi, Illari, Muresu, Narcisa, Puci, Mariangela V., Saderi, Laura, Del Rio, Arcadia, Cossu, Andrea, Muroni, Maria R., Castriciano, Santina, Martinelli, Marianna, Cocuzza, Clementina E., Sotgiu, Giovanni, and Piana, Andrea

Abstract

Background: Given the diagnostic accuracy of HPV-DNA tests in terms of self-collected samples, in order to implement self-sampling in cervical screening programs, the standardization of the pre-analytical phase, including decisions concerning the choice of medium, the volume of elution, and storage conditions, are necessary, in addition to understanding the potential factors involved in acceptability by women. On this basis, we carried out a cross-sectional study to assess (i) the stability of dry vaginal self-collected samples stored at room temperature for up to 4 weeks after elution in 2 mL of eNat® (Copan) medium, and (ii) the acceptability of self-collection in enrolled women. Methods: 185 women were enrolled in the LILT (Italian League Against Tumors) regional project. A self-sampling kit, including a dry FLOQSwab® (Copan), instructions for use, and a satisfaction questionnaire, were supplied for each woman and sent by mail to the laboratory. The HPV-DNA test was carried out using the AnyplexTM II HPV HR (Seegene) kit. To evaluate the specimen’s stability, 185 dry vaginal swabs were eluted in eNat®, a lyses-based molecular medium and tested for HPV detection at two different time points (<6 days and 1 month after elution). The Cohen’s Kappa coefficients and McNemar test were used to assess the agreement of HPV-DNA at different times. Results: We found high agreement in terms of HPV-DNA results among the samples tested at two different time points (Cohen K = 0.98; p < 0.0001). Moreover, most of the women found it easy to use self-collection devices and the pictorial instructions clear to understand. Approximately half of the enrolled women declared preferring self-sampling to clinician-collected methods. Conclusion: Our results display the high reliability and accuracy of HPV-DNA tests using dry vaginal self-collection FLOQSwabs® devices eluted in 2 mL of molecular medium. The analysis of the questionnaire showed a high acceptability of self-collection am

Published
2023

30. Evaluation of Human Papilloma Virus (HPV) Genotyping and Viral Load Determination as Diagnostic Biomarkers of Cervical Cancer Risk

Author

Martinelli, M, Giubbi, C, Saderi, L, Musumeci, R, Perdoni, F, Leone, B, Fruscio, R, Landoni, F, Piana, A, Sotgiu, G, Cocuzza, C, Martinelli, Marianna, Giubbi, Chiara, Saderi, Laura, Musumeci, Rosario, Perdoni, Federica, Leone, Biagio Eugenio, Fruscio, Robert, Landoni, Fabio, Piana, Andrea, Sotgiu, Giovanni, Cocuzza, Clementina Elvezia, Martinelli, M, Giubbi, C, Saderi, L, Musumeci, R, Perdoni, F, Leone, B, Fruscio, R, Landoni, F, Piana, A, Sotgiu, G, Cocuzza, C, Martinelli, Marianna, Giubbi, Chiara, Saderi, Laura, Musumeci, Rosario, Perdoni, Federica, Leone, Biagio Eugenio, Fruscio, Robert, Landoni, Fabio, Piana, Andrea, Sotgiu, Giovanni, and Cocuzza, Clementina Elvezia

Abstract

HPV testing in cervical cancer screening programs offers the possibility of introducing molecular standardized biomarkers for the triage of HPV-positive women. This study aimed to evaluate the role of HPV genotyping and viral load as possible diagnostic biomarkers of high-grade cervical lesions (CIN2+) by performing a preliminary evaluation of a new HPV test. Cervical specimens were obtained from 200 women referred for a colposcopy. Samples were tested using both AnyplexTM II HR-HPV as well as OncoPredict HPV® Screening (SCR) and quantitative typing (QT). Using a cycle threshold cutoff (Ct) of 36.8 for the SCR assay and 1.27 log10 (viral copies/104 cells) for the QT assay, relative clinical sensitivity for CIN2+ and relative clinical specificity for CIN2− as compared to AnyplexTM II HR-HPV were, respectively, 0.92 and 1.00 for SCR and 1.35 and 1.24 for QT. The distribution of high-risk HPV (HR-HPV) genotypes (p = 0.009) as well as the viral copy numbers (CIN2−: 3.7 log10 (viral copies/104 human cells); CIN2+: 4.3 log10 (viral copies/104 human cells); p = 0.047) were found to differ in women with high- and low-grade cervical lesions, suggesting a possible role of HPV genotyping and normalized viral load as potential biomarkers to identify women at increased risk of cervical lesions.

Published
2023

31. Intracellular Nanosensing and Nanodelivery by PMMA Nanoparticles

Author

Giannetti, A., Baldini, F., Ballestri, M., Ghini, G., Giambastiani, G., Guerrini, A., Sotgiu, G., Tombelli, S., Trono, C., Tuci, G., Varchi, G., Baldini, Francesco, editor, D’Amico, Arnaldo, editor, Di Natale, Corrado, editor, Siciliano, Pietro, editor, Seeber, Renato, editor, De Stefano, Luca, editor, Bizzarri, Ranieri, editor, and Andò, Bruno, editor

Published
2014
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32. Factors influencing delayed discharge after day-surgery laparoscopic cholecystectomy: the DeDiLaCo study protocol.

Author

CILLARA, N., FRANSVEA, P., SECHI, R., CICALÒ, E., SOTGIU, G., PROVENZANO, M., POILLUCCI, G., AGNES, A., and PODDA, M.

Abstract

OBJECTIVE: Laparoscopic cholecystectomy (LC) is the gold standard for most benign gallbladder diseases. Early discharge (<24 hours) has the same outcomes as longer (>24 hours) hospital stay. Nevertheless, the rate of delayed discharge >24 hours range from 4.6% to 37%. The primary endpoint of this Italian nationwide study is to analyze the prevalence of patients undergoing elective LC who experienced a delayed discharge >24 hours and identify potential limiting factors of early discharge. Results from these analyses will be used to select patients who can be safely discharged on the same day after surgery. Secondary endpoints will be to evaluate the patient's quality of life (QoL), assess the direct health costs associated with late discharge, and quantify the patient's involvement in the treatment process. PATIENTS AND METHODS: This prospective, observational study was conducted following a resident-led model and the Standard Protocol Items: Recommendations for Interventional Trials (SPIRIT) guidelines. All patients were treated according to the local hospital protocol and received routine care as standard therapy. RESULTS: We expected to obtain the enrollment of at least 500 patients based on an assumed difference in discharge delay between the reference and the recruitable population of 6% and the identification of factors related to discharge failure within 24 h. Early discharge after LC leads to advantages both in terms of clinical outcomes and quality of life of the patient, and it is highly effective in terms of health costs and shortening the waiting list. However, clinical reality differs from the results of randomized studies by a complex series of non-objectionable real-world data influencing treatment plans. Therefore, we expected to identify independent predictors and factors of failure of early discharge. CONCLUSIONS: Clinical reality often differs from randomized trial results. In Italy, the vast majority of delayed discharges after LC may not be related to surgery and can be prevented both with logistical reorganization and with a readjustment of the trust reimbursement policies. [ABSTRACT FROM AUTHOR]

Published
2023

35. Role of Asthma School in disease management

Author

Zappa, M, primary, Visca, D, additional, Pignatti, P, additional, Ardesi, F, additional, Centis, R, additional, Zampogna, E, additional, Cherubino, F, additional, Oliva, F M, additional, Saderi, L, additional, Sotgiu, G, additional, Migliori, G B, additional, and Spanevello, A, additional

Published
2022
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39. Aspiration Risk Factors, Microbiology, and Empiric Antibiotics for Patients Hospitalized With Community-Acquired Pneumonia

Author

Marin-Corral, J, Pascual-Guardia, S, Amati, F, Aliberti, S, Masclans, J, Soni, N, Rodriguez, A, Sibila, O, Sanz, F, Sotgiu, G, Anzueto, A, Dimakou, K, Petrino, R, van de Garde, E, Restrepo, M, Aruj, P, Attorri, S, Barimboim, E, Caeiro, J, Garzon, M, Cambursano, V, Adrian Ceccato, V, Chertcoff, J, Lascar, F, Di Tulio, F, Diaz, A, de Vedia, L, Ganaha, M, Lambert, S, Lopardo, G, Luna, C, Malberti, A, Morcillo, N, Tartara, S, Pensotti, C, Pereyra, B, Scapellato, P, Stagnaro, J, Shah, S, Lotsch, F, Thalhammer, F, Anseeuw, K, Francois, C, Van Braeckel, E, Vincent, J, Djimon, M, Bashi, J, Dodo, R, Nouer, S, Chipev, P, Encheva, M, Miteva, D, Petkova, D, Balkissou, A, Pefura Yone, E, Mbatchou Ngahane, B, Shen, N, Xu, J, Bustamante Rico, C, Buitrago, R, Pereira Paternina, F, Kayembe Ntumba, J, Carevic, V, Jakopovic, M, Jankovic, M, Matkovic, Z, Mitrecic, I, Bouchy Jacobsson, M, Christensen, A, Heitmann Bodtger, U, Meyer, C, Jensen, A, Baunbaek-knudsen, G, Petersen, P, Andersen, S, El-Said Abd El-Wahhab, I, Morsy, N, Shafiek, H, Sobh, E, Abdulsemed, K, Bertrand, F, Brun-Buisson, C, de Montmollin, E, Fartoukh, M, Messika, J, Tattevin, P, Khoury, A, Ebruke, B, Dreher, M, Kolditz, M, Meisinger, M, Pletz, M, Hagel, S, Rupp, J, Schaberg, T, Spielmanns, M, Creutz, P, Suttorp, N, Siaw-Lartey, B, Papapetrou, D, Tsigou, E, Ampazis, D, Kaimakamis, E, Bhatia, M, Dhar, R, D'Souza, G, Garg, R, Koul, P, Mahesh, P, Jayaraj, B, Narayan, K, Udnur, H, Krishnamurthy, S, Kant, S, Swarnakar, R, Limaye, S, Salvi, S, Golshani, K, Keatings, V, Martin-Loeches, I, Maor, Y, Strahilevitz, J, Faverio, P, Battaglia, S, Carrabba, M, Ceriana, P, Confalonieri, M, Monforte, A, Del Prato, B, De Rosa, M, Fantini, R, Fiorentino, G, Gammino, M, Menzella, F, Milani, G, Nava, S, Palmiero, G, Gabrielli, B, Rossi, P, Sorino, C, Steinhilber, G, Zanforlin, A, San Luca, O, Franzetti, F, Carugati, M, Morosi, M, Monge, E, Carone, M, Patella, V, Scarlata, S, Comel, A, Kurahashi, K, Bacha, Z, Ugalde, D, Zuniga, O, Villegas, J, Medenica, M, Mihsra, D, Shrestha, P, Ridgeon, E, Awokola, B, Adefuye Bolanle Olufunlola, O, Olumide, S, Ukwaja, K, Irfan, M, Minarowski, L, Szymon, S, Froes, F, Leuschner, P, Meireles, M, Ferrao, C, Neves, J, Abel, S, Ravara, S, Brocovschii, V, Rusu, D, Toma, C, Chirita, D, Dorobat, C, Birkun, A, Kaluzhenina, A, Almotairi, A, Ali Bukhary, Z, Edathodu, J, Fathy, A, Abdulaziz Enani, A, Mohamed, N, Memon, J, Bella, A, Bogdanovic, S, Milenkovic, B, Pesut, D, Borderias, L, Bordon Garcia, N, Alarcon, H, Cilloniz, C, Torres, A, Diaz-Brito, V, Casas, X, Gonzalez, A, Fernandez-Almira, M, Interna, M, Gallego, M, Gaspar-GarcIa, I, Gonzalez del Castillo, J, Victoria, P, Martinez, E, Malo de Molina, R, Marcos, P, Menendez, R, Pando-Sandoval, A, Aymerich, C, Lacoma de la Torre, A, Garcia-Olive, I, Rello, J, Moyano, S, Rodrigo-Troyano, A, Sole-Violan, J, Uranga, A, van Boven, J, Torra, E, Pujol, J, Feldman, C, Yum, H, Arnauld Attannon Fiogbe, I, Yangui, F, Bilaceroglu, S, Levent Dalar, I, Yilmaz, U, Bogomolov, A, Elahi, N, Dhasmana, D, Feneley, A, Hill, A, Rudran, B, Ruiz-Buitrago, S, Campbell, M, Whitaker, P, Youzguin, A, Singanayagam, A, Hancock, C, Villafuerte, D, Allen, K, Brito, V, Dietz, J, Dysart, C, Kellie, S, Ricardo, A, Meier, G, Gaga, M, Holland, T, Bergin, S, Kheir, F, Landmeier, M, Lois, M, Nair, G, Patel, H, Reyes, K, Rodriguez-Cintron, W, Saito, S, Noda, J, Hinojosa, C, Levine, S, Reyes, L, Angel, L, Whitlow, K, Hipskind, J, Sukhija, K, Totten, V, Wunderink, R, Shah, R, Mateyo, K, Noriega, L, Alvarado, E, Aman, M, Labra, L, Marin-Corral J., Pascual-Guardia S., Amati F., Aliberti S., Masclans J. R., Soni N., Rodriguez A., Sibila O., Sanz F., Sotgiu G., Anzueto A., Dimakou K., Petrino R., van de Garde E., Restrepo M. I., Aruj P. K., Attorri S., Barimboim E., Caeiro J. P., Garzon M. I., Cambursano V. H., Adrian Ceccato V. H. D. C. A., Chertcoff J., Lascar F., Di Tulio F., Diaz A. C., de Vedia L., Ganaha M. C., Lambert S., Lopardo G., Luna C. M., Malberti A. G., Morcillo N., Tartara S., Pensotti C., Pereyra B., Scapellato P. G., Stagnaro J. P., Shah S., Lotsch F., Thalhammer F., Anseeuw K., Francois C. A., Van Braeckel E., Vincent J. L., Djimon M. Z., Bashi J., Dodo R., Nouer S. A., Chipev P., Encheva M., Miteva D., Petkova D., Balkissou A. D., Pefura Yone E. W., Mbatchou Ngahane B. H., Shen N., Xu J. -F., Bustamante Rico C. A., Buitrago R., Pereira Paternina F. J., Kayembe Ntumba J. -M., Carevic V. V., Jakopovic M., Jankovic M., Matkovic Z., Mitrecic I., Bouchy Jacobsson M. -L., Christensen A. B., Heitmann Bodtger U. C., Meyer C. N., Jensen A. V., Baunbaek-knudsen G., Petersen P. T., Andersen S., El-Said Abd El-Wahhab I., Morsy N. E., Shafiek H., Sobh E., Abdulsemed K. A., Bertrand F., Brun-Buisson C., de Montmollin E., Fartoukh M., Messika J., Tattevin P., Khoury A., Ebruke B., Dreher M., Kolditz M., Meisinger M., Pletz M. W., Hagel S., Rupp J., Schaberg T., Spielmanns M., Creutz P., Suttorp N., Siaw-Lartey B., Papapetrou D., Tsigou E., Ampazis D., Kaimakamis E., Bhatia M., Dhar R., D'Souza G., Garg R., Koul P. A., Mahesh P. A., Jayaraj B. S., Narayan K. V., Udnur H. B., Krishnamurthy S. B., Kant S., Swarnakar R., Limaye S., Salvi S., Golshani K., Keatings V. M., Martin-Loeches I., Maor Y., Strahilevitz J., Faverio P., Battaglia S., Carrabba M., Ceriana P., Confalonieri M., Monforte A. D., Del Prato B., De Rosa M., Fantini R., Fiorentino G., Gammino M. A., Menzella F., Milani G., Nava S., Palmiero G., Gabrielli B., Rossi P., Sorino C., Steinhilber G., Zanforlin A., San Luca O., Franzetti F., Carugati M., Morosi M., Monge E., Carone M., Patella V., Scarlata S., Comel A., Kurahashi K., Bacha Z. A., Ugalde D. B., Zuniga O. C., Villegas J. F., Medenica M., Mihsra D. R., Shrestha P., Ridgeon E., Awokola B. I., Adefuye Bolanle Olufunlola O. N. O., Olumide S., Ukwaja K. N., Irfan M., Minarowski L., Szymon S., Froes F., Leuschner P., Meireles M., Ferrao C., Neves J., Abel Salazar, Ravara S. B., Brocovschii V., Rusu D., Toma C., Chirita D., Dorobat C. M., Birkun A., Kaluzhenina A., Almotairi A., Ali Bukhary Z. A., Edathodu J., Fathy A., Abdulaziz Enani A. M., Mohamed N. E., Memon J. U., Bella A., Bogdanovic S. N., Milenkovic B., Pesut D., Borderias L., Bordon Garcia N. M., Alarcon H. C., Cilloniz C., Torres A., Diaz-Brito V., Casas X., Gonzalez A. E., Fernandez-Almira M. L., Interna M., Gallego M., Gaspar-GarcIa I., Gonzalez del Castillo J., Victoria P. J., Martinez E. L., Malo de Molina R., Marcos P. J., Menendez R., Pando-Sandoval A., Aymerich C. P., Lacoma de la Torre A., Garcia-Olive I., Rello J., Moyano S., Rodrigo-Troyano A., Sole-Violan J., Uranga A., van Boven J. F., Torra E. V., Pujol J. A., Feldman C., Yum H. K., Arnauld Attannon Fiogbe I. U., Yangui F., Bilaceroglu S., Levent Dalar I. D., Yilmaz U., Bogomolov A., Elahi N., Dhasmana D. J., Feneley A., Hill A. T., Rudran B., Ruiz-Buitrago S., Campbell M., Whitaker P., Youzguin A., Singanayagam A., Hancock C., Villafuerte D., Allen K. S., Brito V., Dietz J., Dysart C. E., Kellie S. M., Ricardo A. Franco-Sadud C. J., Meier G., Gaga M., Holland T. L., Bergin S. P., Kheir F., Landmeier M., Lois M., Nair G. B., Patel H., Reyes K., Rodriguez-Cintron W., Saito S., Noda J., Hinojosa C. I., Levine S. M., Reyes L. F., Angel L. F., Whitlow K. S., Hipskind J., Sukhija K., Totten V., Wunderink R. G., Shah R. D., Mateyo K. J., Noriega L., Alvarado E., Aman M., Labra L., Marin-Corral, J, Pascual-Guardia, S, Amati, F, Aliberti, S, Masclans, J, Soni, N, Rodriguez, A, Sibila, O, Sanz, F, Sotgiu, G, Anzueto, A, Dimakou, K, Petrino, R, van de Garde, E, Restrepo, M, Aruj, P, Attorri, S, Barimboim, E, Caeiro, J, Garzon, M, Cambursano, V, Adrian Ceccato, V, Chertcoff, J, Lascar, F, Di Tulio, F, Diaz, A, de Vedia, L, Ganaha, M, Lambert, S, Lopardo, G, Luna, C, Malberti, A, Morcillo, N, Tartara, S, Pensotti, C, Pereyra, B, Scapellato, P, Stagnaro, J, Shah, S, Lotsch, F, Thalhammer, F, Anseeuw, K, Francois, C, Van Braeckel, E, Vincent, J, Djimon, M, Bashi, J, Dodo, R, Nouer, S, Chipev, P, Encheva, M, Miteva, D, Petkova, D, Balkissou, A, Pefura Yone, E, Mbatchou Ngahane, B, Shen, N, Xu, J, Bustamante Rico, C, Buitrago, R, Pereira Paternina, F, Kayembe Ntumba, J, Carevic, V, Jakopovic, M, Jankovic, M, Matkovic, Z, Mitrecic, I, Bouchy Jacobsson, M, Christensen, A, Heitmann Bodtger, U, Meyer, C, Jensen, A, Baunbaek-knudsen, G, Petersen, P, Andersen, S, El-Said Abd El-Wahhab, I, Morsy, N, Shafiek, H, Sobh, E, Abdulsemed, K, Bertrand, F, Brun-Buisson, C, de Montmollin, E, Fartoukh, M, Messika, J, Tattevin, P, Khoury, A, Ebruke, B, Dreher, M, Kolditz, M, Meisinger, M, Pletz, M, Hagel, S, Rupp, J, Schaberg, T, Spielmanns, M, Creutz, P, Suttorp, N, Siaw-Lartey, B, Papapetrou, D, Tsigou, E, Ampazis, D, Kaimakamis, E, Bhatia, M, Dhar, R, D'Souza, G, Garg, R, Koul, P, Mahesh, P, Jayaraj, B, Narayan, K, Udnur, H, Krishnamurthy, S, Kant, S, Swarnakar, R, Limaye, S, Salvi, S, Golshani, K, Keatings, V, Martin-Loeches, I, Maor, Y, Strahilevitz, J, Faverio, P, Battaglia, S, Carrabba, M, Ceriana, P, Confalonieri, M, Monforte, A, Del Prato, B, De Rosa, M, Fantini, R, Fiorentino, G, Gammino, M, Menzella, F, Milani, G, Nava, S, Palmiero, G, Gabrielli, B, Rossi, P, Sorino, C, Steinhilber, G, Zanforlin, A, San Luca, O, Franzetti, F, Carugati, M, Morosi, M, Monge, E, Carone, M, Patella, V, Scarlata, S, Comel, A, Kurahashi, K, Bacha, Z, Ugalde, D, Zuniga, O, Villegas, J, Medenica, M, Mihsra, D, Shrestha, P, Ridgeon, E, Awokola, B, Adefuye Bolanle Olufunlola, O, Olumide, S, Ukwaja, K, Irfan, M, Minarowski, L, Szymon, S, Froes, F, Leuschner, P, Meireles, M, Ferrao, C, Neves, J, Abel, S, Ravara, S, Brocovschii, V, Rusu, D, Toma, C, Chirita, D, Dorobat, C, Birkun, A, Kaluzhenina, A, Almotairi, A, Ali Bukhary, Z, Edathodu, J, Fathy, A, Abdulaziz Enani, A, Mohamed, N, Memon, J, Bella, A, Bogdanovic, S, Milenkovic, B, Pesut, D, Borderias, L, Bordon Garcia, N, Alarcon, H, Cilloniz, C, Torres, A, Diaz-Brito, V, Casas, X, Gonzalez, A, Fernandez-Almira, M, Interna, M, Gallego, M, Gaspar-GarcIa, I, Gonzalez del Castillo, J, Victoria, P, Martinez, E, Malo de Molina, R, Marcos, P, Menendez, R, Pando-Sandoval, A, Aymerich, C, Lacoma de la Torre, A, Garcia-Olive, I, Rello, J, Moyano, S, Rodrigo-Troyano, A, Sole-Violan, J, Uranga, A, van Boven, J, Torra, E, Pujol, J, Feldman, C, Yum, H, Arnauld Attannon Fiogbe, I, Yangui, F, Bilaceroglu, S, Levent Dalar, I, Yilmaz, U, Bogomolov, A, Elahi, N, Dhasmana, D, Feneley, A, Hill, A, Rudran, B, Ruiz-Buitrago, S, Campbell, M, Whitaker, P, Youzguin, A, Singanayagam, A, Hancock, C, Villafuerte, D, Allen, K, Brito, V, Dietz, J, Dysart, C, Kellie, S, Ricardo, A, Meier, G, Gaga, M, Holland, T, Bergin, S, Kheir, F, Landmeier, M, Lois, M, Nair, G, Patel, H, Reyes, K, Rodriguez-Cintron, W, Saito, S, Noda, J, Hinojosa, C, Levine, S, Reyes, L, Angel, L, Whitlow, K, Hipskind, J, Sukhija, K, Totten, V, Wunderink, R, Shah, R, Mateyo, K, Noriega, L, Alvarado, E, Aman, M, Labra, L, Marin-Corral J., Pascual-Guardia S., Amati F., Aliberti S., Masclans J. R., Soni N., Rodriguez A., Sibila O., Sanz F., Sotgiu G., Anzueto A., Dimakou K., Petrino R., van de Garde E., Restrepo M. I., Aruj P. K., Attorri S., Barimboim E., Caeiro J. P., Garzon M. I., Cambursano V. H., Adrian Ceccato V. H. D. C. A., Chertcoff J., Lascar F., Di Tulio F., Diaz A. C., de Vedia L., Ganaha M. C., Lambert S., Lopardo G., Luna C. M., Malberti A. G., Morcillo N., Tartara S., Pensotti C., Pereyra B., Scapellato P. G., Stagnaro J. P., Shah S., Lotsch F., Thalhammer F., Anseeuw K., Francois C. A., Van Braeckel E., Vincent J. L., Djimon M. Z., Bashi J., Dodo R., Nouer S. A., Chipev P., Encheva M., Miteva D., Petkova D., Balkissou A. D., Pefura Yone E. W., Mbatchou Ngahane B. H., Shen N., Xu J. -F., Bustamante Rico C. A., Buitrago R., Pereira Paternina F. J., Kayembe Ntumba J. -M., Carevic V. V., Jakopovic M., Jankovic M., Matkovic Z., Mitrecic I., Bouchy Jacobsson M. -L., Christensen A. B., Heitmann Bodtger U. C., Meyer C. N., Jensen A. V., Baunbaek-knudsen G., Petersen P. T., Andersen S., El-Said Abd El-Wahhab I., Morsy N. E., Shafiek H., Sobh E., Abdulsemed K. A., Bertrand F., Brun-Buisson C., de Montmollin E., Fartoukh M., Messika J., Tattevin P., Khoury A., Ebruke B., Dreher M., Kolditz M., Meisinger M., Pletz M. W., Hagel S., Rupp J., Schaberg T., Spielmanns M., Creutz P., Suttorp N., Siaw-Lartey B., Papapetrou D., Tsigou E., Ampazis D., Kaimakamis E., Bhatia M., Dhar R., D'Souza G., Garg R., Koul P. A., Mahesh P. A., Jayaraj B. S., Narayan K. V., Udnur H. B., Krishnamurthy S. B., Kant S., Swarnakar R., Limaye S., Salvi S., Golshani K., Keatings V. M., Martin-Loeches I., Maor Y., Strahilevitz J., Faverio P., Battaglia S., Carrabba M., Ceriana P., Confalonieri M., Monforte A. D., Del Prato B., De Rosa M., Fantini R., Fiorentino G., Gammino M. A., Menzella F., Milani G., Nava S., Palmiero G., Gabrielli B., Rossi P., Sorino C., Steinhilber G., Zanforlin A., San Luca O., Franzetti F., Carugati M., Morosi M., Monge E., Carone M., Patella V., Scarlata S., Comel A., Kurahashi K., Bacha Z. A., Ugalde D. B., Zuniga O. C., Villegas J. F., Medenica M., Mihsra D. R., Shrestha P., Ridgeon E., Awokola B. I., Adefuye Bolanle Olufunlola O. N. O., Olumide S., Ukwaja K. N., Irfan M., Minarowski L., Szymon S., Froes F., Leuschner P., Meireles M., Ferrao C., Neves J., Abel Salazar, Ravara S. B., Brocovschii V., Rusu D., Toma C., Chirita D., Dorobat C. M., Birkun A., Kaluzhenina A., Almotairi A., Ali Bukhary Z. A., Edathodu J., Fathy A., Abdulaziz Enani A. M., Mohamed N. E., Memon J. U., Bella A., Bogdanovic S. N., Milenkovic B., Pesut D., Borderias L., Bordon Garcia N. M., Alarcon H. C., Cilloniz C., Torres A., Diaz-Brito V., Casas X., Gonzalez A. E., Fernandez-Almira M. L., Interna M., Gallego M., Gaspar-GarcIa I., Gonzalez del Castillo J., Victoria P. J., Martinez E. L., Malo de Molina R., Marcos P. J., Menendez R., Pando-Sandoval A., Aymerich C. P., Lacoma de la Torre A., Garcia-Olive I., Rello J., Moyano S., Rodrigo-Troyano A., Sole-Violan J., Uranga A., van Boven J. F., Torra E. V., Pujol J. A., Feldman C., Yum H. K., Arnauld Attannon Fiogbe I. U., Yangui F., Bilaceroglu S., Levent Dalar I. D., Yilmaz U., Bogomolov A., Elahi N., Dhasmana D. J., Feneley A., Hill A. T., Rudran B., Ruiz-Buitrago S., Campbell M., Whitaker P., Youzguin A., Singanayagam A., Hancock C., Villafuerte D., Allen K. S., Brito V., Dietz J., Dysart C. E., Kellie S. M., Ricardo A. Franco-Sadud C. J., Meier G., Gaga M., Holland T. L., Bergin S. P., Kheir F., Landmeier M., Lois M., Nair G. B., Patel H., Reyes K., Rodriguez-Cintron W., Saito S., Noda J., Hinojosa C. I., Levine S. M., Reyes L. F., Angel L. F., Whitlow K. S., Hipskind J., Sukhija K., Totten V., Wunderink R. G., Shah R. D., Mateyo K. J., Noriega L., Alvarado E., Aman M., and Labra L.

Abstract

Background: Aspiration community-acquired pneumonia (ACAP) and community-acquired pneumonia (CAP) in patients with aspiration risk factors (AspRFs) are infections associated with anaerobes, but limited evidence suggests their pathogenic role. Research Question: What are the aspiration risk factors, microbiology patterns, and empiric anti-anaerobic use in patients hospitalized with CAP? Study Design and Methods: This is a secondary analysis of GLIMP, an international, multicenter, point-prevalence study of adults hospitalized with CAP. Patients were stratified into three groups: (1) ACAP, (2) CAP/AspRF+ (CAP with AspRF), and (3) CAP/AspRF- (CAP without AspRF). Data on demographics, comorbidities, microbiological results, and anti-anaerobic antibiotics were analyzed in all groups. Patients were further stratified in severe and nonsevere CAP groups. Results: We enrolled 2,606 patients with CAP, of which 193 (7.4%) had ACAP. Risk factors independently associated with ACAP were male, bedridden, underweight, a nursing home resident, and having a history of stroke, dementia, mental illness, and enteral tube feeding. Among non-ACAP patients, 1,709 (70.8%) had CAP/AspRF+ and 704 (29.2%) had CAP/AspRF-. Microbiology patterns including anaerobes were similar between CAP/AspRF-, CAP/AspRF+ and ACAP (0.0% vs 1.03% vs 1.64%). Patients with severe ACAP had higher rates of total gram-negative bacteria (64.3% vs 44.3% vs 33.3%, P =.021) and lower rates of total gram-positive bacteria (7.1% vs 38.1% vs 50.0%, P <.001) when compared with patients with severe CAP/AspRF+ and severe CAP/AspRF-, respectively. Most patients (>50% in all groups) independent of AspRFs or ACAP received specific or broad-spectrum anti-anaerobic coverage antibiotics. Interpretation: Hospitalized patients with ACAP or CAP/AspRF+ had similar anaerobic flora compared with patients without aspiration risk factors. Gram-negative bacteria were more prevalent in patients with severe ACAP. Despite having similar

Published
2021

43. Chemical CeO2-Based Buffer Layers for Fe(Se,Te) Films

Author

Piperno, L., primary, Vannozzi, A., additional, Pinto, V., additional, Augieri, A., additional, Armenio, A. Angisani, additional, Rizzo, F., additional, Mancini, A., additional, Rufoloni, A., additional, Celentano, G., additional, Braccini, V., additional, Cialone, M., additional, Iebole, M., additional, Manca, N., additional, Martinelli, A., additional, Putti, M., additional, Sotgiu, G., additional, and Meledin, A., additional

Published
2022
Full Text
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44. Infections in lung transplanted patients: A review

Author

Dettori, M., primary, Riccardi, N., additional, Canetti, D., additional, Antonello, R.M., additional, Piana, A.F., additional, Palmieri, A., additional, Castiglia, P., additional, Azara, A.A., additional, Masia, M.D., additional, Porcu, A., additional, Ginesu, G.C., additional, Cossu, M.L., additional, Conti, M., additional, Pirina, P., additional, Fois, A., additional, Maida, I., additional, Madeddu, G., additional, Babudieri, S., additional, Saderi, L., additional, and Sotgiu, G., additional

Published
2022
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45. Travel-Related Typhoid Fever: Narrative Review of the Scientific Literature

Author

Muresu, N, Sotgiu, G, Are, B, Cossu, A, Cocuzza, C, Martinelli, M, Babudieri, S, Are, R, Dettori, M, Azara, A, Saderi, L, Piana, A, Muresu N., Sotgiu G., Are B. M., Cossu A., Cocuzza C., Martinelli M., Babudieri S., Are R., Dettori M., Azara A., Saderi L., Piana A., Muresu, N, Sotgiu, G, Are, B, Cossu, A, Cocuzza, C, Martinelli, M, Babudieri, S, Are, R, Dettori, M, Azara, A, Saderi, L, Piana, A, Muresu N., Sotgiu G., Are B. M., Cossu A., Cocuzza C., Martinelli M., Babudieri S., Are R., Dettori M., Azara A., Saderi L., and Piana A.

Abstract

Enteric fever is a foodborne infectious disease caused by Salmonella enterica serotypes Typhi and Paratyphi A, B and C. The high incidence in low income countries can increase the risk of disease in travelers coming from high income countries. Pre-travel health advice on hygiene and sanitation practices and vaccines can significantly reduce the risk of acquiring infections. Although the majority of the cases are self-limiting, life-threatening complications can occur. Delayed diagnosis and cases of infections caused by multi-drug resistant strains can complicate the clinical management and affect the prognosis. More international efforts are needed to reduce the burden of disease in low income countries, indirectly reducing the risk of travelers in endemic settings. Surveillance activities can help monitor the epidemiology of cases caused by drug-susceptible and resistant strains.

Published
2020

46. Utility and safety of bronchoscopy during the SARS-CoV-2 outbreak in Italy: A retrospective, multicentre study

Author

Mondoni, M, Papa, G, Rinaldo, R, Faverio, P, Marruchella, A, D'Arcangelo, F, Pesci, A, Pasini, S, Henchi, S, Cipolla, G, Tarantini, F, Giuliani, L, Di Marco, F, Saracino, L, Tomaselli, S, Corsico, A, Gasparini, S, Bonifazi, M, Zuccatosta, L, Saderi, L, Pellegrino, G, Centanni, M, Sotgiu, G, Carlucci, P, Mondoni M., Papa G. F. S., Rinaldo R., Faverio P., Marruchella A., D'Arcangelo F., Pesci A., Pasini S., Henchi S., Cipolla G., Tarantini F., Giuliani L., Di Marco F., Saracino L., Tomaselli S., Corsico A., Gasparini S., Bonifazi M., Zuccatosta L., Saderi L., Pellegrino G., Centanni M. D. S., Sotgiu G., Carlucci P., Mondoni, M, Papa, G, Rinaldo, R, Faverio, P, Marruchella, A, D'Arcangelo, F, Pesci, A, Pasini, S, Henchi, S, Cipolla, G, Tarantini, F, Giuliani, L, Di Marco, F, Saracino, L, Tomaselli, S, Corsico, A, Gasparini, S, Bonifazi, M, Zuccatosta, L, Saderi, L, Pellegrino, G, Centanni, M, Sotgiu, G, Carlucci, P, Mondoni M., Papa G. F. S., Rinaldo R., Faverio P., Marruchella A., D'Arcangelo F., Pesci A., Pasini S., Henchi S., Cipolla G., Tarantini F., Giuliani L., Di Marco F., Saracino L., Tomaselli S., Corsico A., Gasparini S., Bonifazi M., Zuccatosta L., Saderi L., Pellegrino G., Centanni M. D. S., Sotgiu G., and Carlucci P.

Published
2020

47. Distribution of hpv genotypes in patients with a diagnosis of anal cancer in an Italian region

Author

Muresu, N, Sotgiu, G, Saderi, L, Sechi, I, Cossu, A, Marras, V, Meloni, M, Martinelli, M, Cocuzza, C, Tanda, F, Piana, A, Muresu N., Sotgiu G., Saderi L., Sechi I., Cossu A., Marras V., Meloni M., Martinelli M., Cocuzza C., Tanda F., Piana A., Muresu, N, Sotgiu, G, Saderi, L, Sechi, I, Cossu, A, Marras, V, Meloni, M, Martinelli, M, Cocuzza, C, Tanda, F, Piana, A, Muresu N., Sotgiu G., Saderi L., Sechi I., Cossu A., Marras V., Meloni M., Martinelli M., Cocuzza C., Tanda F., and Piana A.

Abstract

Objectives: Anal cancer is a rare disease. However, its incidence is increasing in some population groups. Infection caused by Human Papillomavirus (HPV) is strongly associated with the risk of anal cancer, whose variability depends on samples, histology, and HPV detection methods. The aim of the study was to assess prevalence and distribution of HPV genotypes in patients diagnosed with anal carcinoma. Methods: An observational, retrospective study was carried out in a tertiary care hospital in North Sardinia, Italy. Specimens of anal cancer diagnosed from 2002–2018 were selected. Demographic, epidemiological, and clinical variables were collected to assess their relationship with the occurrence of anal cancer. Results: The overall HPV positivity was 70.0% (21/30), with HPV-16 being the predominant genotype (~85%). The highest prevalence of anal cancer was in patients aged ≥55 years. HPV positivity was higher in women (p-value > 0.05) and in moderately differentiated samples (G2) (p-value < 0.05). p16INK4a and E6-transcript positivity were found in 57% and 24% of the HPV positive samples, respectively. The OS (overall survival) showed a not statistically significant difference in prognosis between HPV positive sand negatives (10, 47.6%, vs. 4, 44.4%; p-value = 0.25). Conclusions: HPV-DNA and p16INK4a positivity confirmed the role of HPV in anal carcinoma. Our findings could support the implementation and scale-up of HPV vaccination in males and females to decrease the incidence of HPV-associated cancers. Further studies are needed to better clarify the prognostic role of HPV/p16 status.

Published
2020

50. Effect on Durability and Electrochemical Response of the Addition of a Non-noble Transition Metal in Mixed Ruthenium and Manganese Oxide Thin-film Electrodes

Author

Sotgiu, G., Orsini, M., de Santis, S., Porcelli, F., Petrucci, E., Sotgiu, G., Orsini, M., de Santis, S., Porcelli, F., and Petrucci, E.

Subjects
TK7885-7895, Computer engineering. Computer hardware, accelerated tests, Chemical engineering, electrochemical performance, TP155-156, ruthenium-based ternary mixtures, thin-film electrodes
Abstract

Mixed metal oxide (MMOx) thin films-based electrodes have been widely used in the treatment of polluted solutions. To limit costs while exploring new electrocatalytic potentials, novel mixtures are being proposed. In this paper, the electrochemical behavior of thin films obtained by co-deposition of Ruthenium, Manganese, and a third transition metal among Copper, Iron, and Cobalt was investigated with the scope of finding optimal compositions and long stability for applications as the anodes in environmental treatments. The binary and ternary oxides coated electrodes were prepared by thermal decomposition on titanium foils from alcoholic solutions. The morphological properties of the thin films were analyzed through scanning electron microscopy (SEM) while the electrochemical performance was evaluated by potentiodynamic polarization curves and cyclic voltammetry (CV). The service time was assessed by accelerated life tests. The results show that the combination of Ruthenium with one or two different metals results in evident modifications of the morphology, both kinetic and thermodynamic electrochemical properties as well as the service lifetime. In particular, a combination of Iron and Manganese was to be excluded because of the poor solubility which affected both the coating homogeneity and electrochemical response. The Cobalt-containing electrodes outperformed the others in terms of stability. The addition of Copper implied a reduced susceptibility to corrosion and higher charge storage.

Published
2021

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