1. Visceral and Subcutaneous Adipose Tissue Diacylglycerol Acyltransferase Activity in Humans
- Author
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Xinguo Hou, Sonja Moser, Geoffrey B. Thompson, Michael G. Sarr, Michael D. Jensen, and Florencia G. Que
- Subjects
Adult ,Male ,medicine.medical_specialty ,Intra-Abdominal Fat ,Endocrinology, Diabetes and Metabolism ,Subcutaneous Fat ,Medicine (miscellaneous) ,Adipose tissue ,Fatty Acids, Nonesterified ,Article ,Gene Expression Regulation, Enzymologic ,chemistry.chemical_compound ,Cytosol ,Endocrinology ,Microsomes ,Adipocyte ,Internal medicine ,Adipocytes ,medicine ,Humans ,Diacylglycerol O-Acyltransferase ,Obesity ,RNA, Messenger ,Aged ,Cell Size ,chemistry.chemical_classification ,Nutrition and Dietetics ,biology ,Triglyceride ,Fatty acid ,Middle Aged ,medicine.disease ,Enzyme assay ,Kinetics ,chemistry ,Microsome ,biology.protein ,Female - Abstract
Diacylglycerol acyltransferase (DGAT) could be a rate limiting step in triglyceride (TG) synthesis as it is the final step in this pathway. As such, between depot differences in DGAT activity could influence regional fat storage. DGAT activity and in vitro rates of direct free fatty acid (FFA) storage were measured in abdominal subcutaneous and omental adipose tissue samples from 12 non-obese (BMI < 30kg/m2) and 23 obese men and women (BMI > 30kg/m2) undergoing elective surgery. DGAT activity was greater in omental than in abdominal subcutaneous adipose tissue from non-obese patients (2.0 ± 0.9 vs. 0.9 ± 0.3 pmol/min/mg lipid, respectively, P = 0.003), but not from obese patients (1.4 ± 0.6 vs. 1.7 ± 0.7 pmol/min/mg lipid, respectively, P = 0.10). DGAT activity per unit adipose weight was negatively correlated with adipocyte size (p
- Published
- 2009
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