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The equilibrium solubility of amrinone in solvent mixtures of methanol + water, ethanol + water, N,N-dimethylformamide (DMF,) + water, and ethylene glycol (EG) + water was determined experimentally...

miR-139-3p exerts tumor-suppressing functions in various cancers. We analyzed and identified that miR-139-3p expression was notably low in gastric cancer (GC) via edgeR differential analysis based on The Cancer Genome Atlas database and quantitative real-time polymerase chain reaction (qRT-PCR) assay. The binding relationship between Kinesin Family Member 18B (KIF18B) and miR-139-3p was predicted by bioinformatics databases, and verified through dual-luciferase assay. Western blot and qRT-PCR results also indicated that miR-139-3p restrained KIF18 expression at mRNA and protein levels. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, wound healing, transwell, flow cytometry assays were introduced to evaluate cell proliferation, migration, invasion, and cell cycle, respectively, where the results indicated that upregulating miR-139-3p inhibited proliferative, migratory, and invasive abilities of GC cells, while caused cell-cycle arrest. Moreover, the results of rescue experiments illustrated that miR-139-3p hampered the progression of GC cells by targeting and suppressing KIF18B. To sum up, we concluded that miR-139-3p suppressed GC progression by targeting KIF18B.

Deoxynivalenol (DON) is a secondary metabolite produced by Fusarium graminearum in grains, food and contaminated feed, which can lead to many adverse health effects to humans and livestock. The degradation of DON in different contaminated grains oxidated by saturated aqueous ozone (80 mg L−1) was monitored by ultra-high-performance liquid chromatography, tandem-quadrupole-detection mass spectrometry (UPLC–TQD-MS). Results suggest that ozone has a significant effect on DON reduction in solution. When 80 mg L−1 gaseous ozone was used to treat 10 mg L−1 of DON solution, the degradation rate of DON reached 83% within 7 min, while the respective detoxification rates of contaminated wheat, corn and bran by saturated aqueous ozone (80 mg L−1) were 74.86%, 70.65% and 76.21 in 10 min. Ozone at 80 mg L−1 was applied on various DON solution concentrations at 1 mg L−1, 10 mg L−1 and 20 mg L−1 in ultra-pure water. In this paper, the degradation procedure for DON is calculated and described by a first-order kinetic equation. At lower levels (20 mg L−1) of aqueous ozone, intermediate degradation products were observed by ultra-ne quickly and effectively degrades DON and toxicity in various contaminated grains in a matter of minutes. Therefore, ozonation is projected to be an effective, fast, and safe method for DON degradation.

Thinking innovation is one of important driving forces to promote scientific and technological progress. Information technology can help humans to brainstorm as many potential ideas and methods as possible for given problems so as to inspire their creative thinking. Knowledge graph provides a structural method to storage human prior knowledge described by concepts and relationships between them in a graph way. The frame and models of Probability Construction Space Theory(PCST) explained the mechanism of human creative thinking and procedure of new ideas generation. Our research work explores generation of new potential methods of detecting and perception geophysical information through simulating the creative thinking of new ideas of aircraft guidance based on the combination of theory and technology of knowledge graph and PCST. One experiment software system has been built to verify feasibility of this novel cognitive model and simulation method presented in our research work.

P3HT-g-C3N4 photocatalysts with high activity have been fabricated by assembling p-type P3HT particles on n-type g-C3N4 nanoplates via a ball milling method. The photocatalytic activity of the P3HT-g-C3N4 photocatalysts for the degradation of MB was 2 times higher than that of pure g-C3N4. The formation of a heterojunction interface of P3HT-g-C3N4 photocatalysts enhanced the separation efficiency of photogenerated electron–hole pairs and resulted in the enhancement of photocatalytic performance. The potential difference in the heterojunction is the main driving force for efficient charge separation and transfer.

Tailored nanostructures offer a new way to facilitate electron–hole separation and offer additional opportunities to generate unique photocatalysts that demonstrate novel light absorption, thermodynamic and kinetic properties. A simple and efficient approach to the synthesis of a large variety of g-C3N4 tailored nanostructures is reported. Herein, NH3 and H2O2 were used as controllable chemical scissors to tailor bulk g-C3N4 to a large variety of g-C3N4 nanostructures, which include exfoliated porous, quantum dot, nanomites and nanospindles. The tailored g-C3N4 shows a photoreactivity of H2 evolution 3.0 (pure water) and 4.1 (saturated KCl solution) times higher than bulk g-C3N4 under λ > 420 nm. We believe this strategy affords new opportunities for structural tuning of X-doped (X = N, S, P, and O) carbon materials, as well as their exploration in catalysis, organic synthesis, nanomedicine and energy storage.

Alveolar epithelial type II (AEII) cells are a key structure and defender in the lung but also are the targets in many lung diseases, including acute respiratory distress syndrome, ventilator-induced lung injury, and pulmonary fibrosis. We sought to establish an optimized method for high yielding and long maintenance of characteristics of primary human AEII cells to facilitate the investigation of the mechanisms of lung diseases at the cellular and molecular levels. Adult human peripheral normal lung tissues of oncologic patients undergoing lung resection were collected. The AEII cells were isolated and identified by the expression of pro-surfactant protein (SP)C, epithelial sodium channel (αENaC) and cytokeratin (CK)-8, the lamellar bodies specific for AEII cells, and confirmed by the histology using electron microscopy. The phenotype of AEII cells was characterized by the expression of surfactant proteins (SP-A, SP-B, SP-C, SP-D), CK-8, KL-6, αENaC, and aquaporin (AQP)-3, which was maintained over 20 days. The biological activity of the primary human AEII cells producing SP-C, cytokines, and intercellular adhesion molecule-1 was vigorous in response to stimulation with tumor necrosis factor-α. We have modified previous methods and optimized a method for isolation of high purity and long maintenance of the human AEII cell phenotype in primary culture. This method provides an important tool for studies aiming at elucidating the molecular mechanisms of lung diseases exclusively in AEII cells.

A new lateral flow immunoassay (LFA) is proposed for qualitative and/or semiquantitative determination of aflatoxin B1 (AFB1), zearalenone (ZEA), deoxynivalenol (DON), and their analogues (AFs, ZEAs, DONs) in cereal samples. Each of the mycotoxin specific antibody was class specific and there was no cross reactivity to other groups of compounds. The visual limits of detection (vLOD) of the strip were 0.03, 1.6, and 10 μg/kg for AFB1, ZEA and DON, respectively. The calculated limits of detection (cLOD) were 0.05, 1, and 3 μg/kg, respectively. Meanwhile the cutoff values were achieved at 1, 50, and 60 μg/kg for AFB1, ZEA and DON, respectively. Recoveries ranged from 80% to 122% and RSD from 5% to 20%. Both the vLOD and cLOD for the three mycotoxins were lower than the EU maximum levels. Analysis of naturally contaminated maize samples resulted in a good agreement between the multiplex LFA and LC-MS/MS (100% for DONs and AFs, and 81% for ZEAs). Careful analysis of the results further explained the general overestimation of LFA compared to chromatographic methods for quantification of mycotoxins.

Two species of Xorides (Moerophora) collected in the northeastern part of China, in which one is new to science, namely Xorides (Moerophora)tuqiangensis Sheng sp. nov., and one, X. (M.) jakovlevi (Kokujev) 1903, is a Chinese new record, are reported. A key is provided to distinguish the Chinese species of this subgenus.

The effectiveness of Norplant implants over a seven year period of continuous use was studied in a multicenter trial. Pregnancy rates were 0.4 per 100 in both year six and year seven. More than 3,600 women completed 6 years and more than 2,400 women completed 7 years. Pregnancy rates increased with weight (p.05) and decreased with age, but in years 6 and 7 combined, the pregnancy rate neither reached nor exceeded 1 per 100 woman years in any 5 year age group or in any 10 kg weight group.The effectiveness of Norplant implants containing a total of 216 mg of levonorgestrel over a 7-year period of continuous use was studied in a multicenter trial. Enrollment continued from 1984 to 1987 with a cut-off in May 1994, by which time all women had completed 7 years of use. The subjects were 18-40 years old with at least 1 living child. More than 3600 women completed 6 years and more than 2400 women completed 7 years. There were 18 pregnancies in the 6th year and 12 in the 7th year. One of these was ectopic. The annual gross pregnancy rates were 0.40 per 100 in both the 6th and 7th year. For both years there was a significant difference in pregnancy rates among women in the different weight groupings (p 0.05), but not in the different age groupings. In years 6 and 7 combined, the pregnancy rate neither reached nor exceeded 1 per 100 woman years in any 5 year age group or in any 10 kg weight group. Only women under age 30 at admission who then weighed 65 or more kg had a pregnancy rate of 1.31 +or- 0.76 per 100 in the 6th and 7th years. No age-weight group had a pregnancy rate as high as 2.0 per 100 in the 2-year period beyond normal use. Cumulative life table pregnancy rates at the end of 5 years had been 1.53 per 100 for all subjects. At the end of 7 years the cumulative rate had increased by less than 1 per 100 to 2.32 per 100. The highest cumulative pregnancy rates were found among the women under age 25 and those weighing 70 or more kg at acceptance. The former group had a cumulative failure rate of 4.7 and the latter a cumulative rate of 6.6 per 100, respectively. This study provides evidence that Norplant implants remain a highly effective contraceptive method for a period of 7 years of continuous use.