Search

Background: Postoperative anxiety is a common surgical complication in older patients. Research has recently linked excessive autophagy to several neurological disorders, including anxiety. This study aimed to determine whether 3-Methyladenine (3-MA) administration reduced anxiety-like behaviors in a mouse model following exploratory abdominal laparotomy. Methods: An abdominal exploratory laparotomy model of postoperative anxiety was established using male C57BL/6 mice aged 20 months. 3-MA (6, 30 and 150 mg/kg) was administered via intracerebroventricular immediately following surgery. The mice were assessed 14 days after surgery using the marble burying, elevated plus maze tests, and local field potential recording in the amygdala. The levels of expression of phosphorylated-Akt, Beclin-1, LC3B, nuclear factor erythroid 2-related factor 2 (Nrf2)-occupied regions in NeuN-positive cells, superoxide dismutase (SOD) activity, malondialdehyde (MDA) and glutathione (GSH) were measured at 24 h after surgery. Results: The injection of 3-MA reversed the increased number of marbles buried, decreased time spent in the open arm, and enhanced θ oscillation power after 14 days of abdominal exploratory laparotomy. In addition, administration of 3-MA reduced the ratio of phosphorylated- to total-Akt, decreased expression in Beclin-1 and LC3B, attenuated MDA levels, and increased the ratio of Nrf2-occupied areas in NeuN-positive cells, SOD activity, and GSH levels under abdominal exploratory laparotomy conditions. Conclusions: 3-MA improved anxiety-like behaviors in aged mice undergoing abdominal exploratory laparotomy by inhibiting excessive autophagy-induced oxidative stress. These results suggest that 3-MA could be an effective treatment for postoperative anxiety.

Smilax china L. is a well-known traditional medicinal plant. In China, it is a common anti-cancer drug that has been inherited for thousands of years. Some in vitro and in vivo studies have confirmed its potential lipid-lowering, anti-inflammatory and anti-ovarian cancer effects. However, there is no research on the material basis and mechanism of the rhizome of Smilax china L. against hepatocellular carcinoma.To explore the material basis and mechanism of scopolin from Smilax china L. against hepatocellular carcinoma.The potential targets and active components of Smilax china L. against hepatocellular carcinoma were screened by transcriptomics, network pharmacology and molecular docking. Microscale Thermophoresis (MST) detection was used to verify the affinity of small molecule compounds with potential proteins and protein-protein interaction. The Extract from HepG2 cells was used to measure the expression of glycolysis-related proteins, glucose consumption and lactate production. The expression of apoptosis-related factors and glycolysis-related proteins in vivo was detected by immunohistochemistry.The glycolysis-related proteins glucose-6-phosphate isomerase (GPI), glycerol-3-phosphate dehydrogenase, mitochondrial (GPD2) and phosphoglycerate kinase 2 (PGK2) screened by transcriptomics, network pharmacology showed strongly binding with scopolin by molecular docking. MST detection has also verified the affinity of scopolin with GPI and GPD2. It was the first time found that Heat shock protein HSP 90-alpha (Hsp90α) bound strongly to GPI and GPD2 in the worldwide, while scopolin was able to affect the interaction between Hsp90α and GPD2. In vitro and in vivo experiments further demonstrated that scopolin may play an anti-cancer role by affecting the stability of tumor-associated proteins. The results showed that scopolin obtained from Smilax china L. could regulate the expression of GPI, GPD2 and PGK2 and inhibit the interaction of protein-protein, reduce the energy metabolism of tumor tissue, thereby inhibit tumor growth.Scopolin obtained from Smilax china L. plays the role of anti-hepatocellular carcinoma by regulating the expression of glycolysis proteins GPI, GPD2 and PGK2. Scopolin could affect the interaction between Hsp90α and GPD2 may provide a novel potential treatment direction for hepatocellular carcinoma.

Subtrochanteric osteotomy of the femur (STO) is a valuable corrective procedure in hip surgeries. However, STO in traditional posterolateral approach usually encounters complications such as postoperative dislocation, bone non-union, and prosthesis failure. Some relevant pathologies and mechanisms have been identified, but there is sparse evidence for verification. The aim of this video in orthopaedic technique is to test our hypothesis of STO in direct anterior approach to total hip arthroplasty in a complicated hip surgery, and to further illustrate the rationality, reproducibility, and superiority of STO in this minimally invasive and enhanced-recovery approach by presenting a standardized and systemic protocol, as well as operational pearls and pitfalls.

The high expression of fatty acid synthase (FAS) in tumor cells is consistent with their elevated requirement for fatty acids for cell membrane synthesis and energy supply to support their almost unlimited proliferation. The expression levels of FAS in tumor cells are related to their proliferation, invasion, and metastasis. This study investigated the possible bioactive ingredients (fraxin, esculetin, scopolin et al.) of Cortex Fraxini and their effects on the interaction between specific proteins. We used microscale thermophoresis (MST) to show that our target protein, FAS (screened by combining transcriptome and network pharmacology), bound to the active compounds in Cortex Fraxini. It was found that FAS bound strongly to Glucose-6-phosphate isomerase (GPI), and that scopolin could affect this interaction by proteomics and MST. The results of this study demonstrate that the active compounds in Cortex Fraxini could play an anti-tumor role by binding to FAS and inhibiting the interactions between FAS and GPI to affect glucose and lipid metabolism, and that the protein pathway is a potential novel target for tumor treatment.

Albiziae Flos (AF) has been experimentally proven to have an antidepressant effect. However, due to the complexity of botanical ingredients, the exact pharmacological mechanism of action of AF in depression has not been completely deciphered. This study used the network pharmacology method to construct a component-target-pathway network to explore the active components and potential mechanisms of action of AF. The methods included collection and screening of chemical components, prediction of depression-associated targets of the active components, gene enrichment, and network construction and analysis. Quercetin and 4 other active components were found to exert antidepressant effects mainly via monoaminergic neurotransmitters and cAMP signaling and neuroactive ligand-receptor interaction pathways. DRD2, HTR1A, and SLC6A4 were identified as important targets of the studied bioactive components of AF. This network pharmacology analysis provides guidance for further study of the antidepressant mechanism of AF.

This study explored the possible bioactive ingredients and target protein of Rostellularia procumbens (L.) Nees. The results of optical turbidimetry revealed that the ethyl acetate extraction obtained from R. procumbens (L.) Nees could inhibit platelet aggregation. Gene chip was used to investigate differentially expressed genes. According to the results of the gene chip, the targets of compounds isolated from the ethyl acetate extraction were predicted by network pharmacology. Computational studies revealed that chinensinaphthol methyl ether and neojusticin B may target the integrin αIIbβ3 protein. The results of Prometheus NT.48 and microscale thermophoresis suggested that the molecular interactions between the two compounds with purified integrin αIIbβ3 protein in the optimal test conditions were coherent with the docking results. To our best knowledge, this is the first report to state that chinensinaphthol methyl ether and neojusticin B target the integrin αIIbβ3 protein.

High-dislocated hip dysplasia is challenging to treat with total hip arthroplasty via the direct anterior approach (DAA). The DAA has potential advantages including optimizing component positioning, enhanced hip stability, and a more rapid postoperative recovery. We present a surgical technique for DAA total hip arthroplasty for hip dysplasia that includes preoperative planning, soft tissue releases, subtrochanteric osteotomy, component placement, and intraoperative nerve monitoring and imaging. This technique provides detailed technical instructions, specifically including pearls and pitfalls, and complication prevention strategies.

On the International Thermonuclear Experimental Reactor superconducting fusion device, the Nb3Sn composite strands have been applied to cable-in-conduit conductor to meet the impact of the magnet field about 12 T. The strain of the Nb3Sn-based conductor main due to Lorentz forces leads to the change of coupling loss time constant. However, the effect exploration of contact properties considering electromagnetic load cycle on coupling loss time constant is insufficient. In order to calculate coupling loss accurately and quickly, a new calculation model of coupling loss time constant was presented in this paper, which is expressed in three parameters, i.e., cabling sequence ratio, contact resistance, and void fraction with electromagnetic force cycle. In this model, not only cabling sequence ratio was established, but also contact resistance and void fraction considering strain resulted from electromagnetic load cycle were obtained. Compared with numerical calculation using Gandalf and traditional method, the error of coupling loss calculated with the combination of cabling sequence ratio, contact resistance, and void fraction under electromagnetic load cycle is smaller, which is closer to the engineering measured value.

Mesangial proliferative glomerulonephritis (MsPGN) is characterized by mesangial cell proliferation, inflammation, and extracellular matrix deposition in the mesangial area, which develops into glomerulosclerosis and contributes to end-stage renal disease. Justicidin B is a bioactive compound isolated from Justicia procumbens L., a traditional herbal remedy that reduces proteinuria in nephritis. However, the mechanism of Justicidin B’s therapeutic effect on MsPGN remains unclear. This study was aimed to explore the positive effect of Justicidin B on MsPGN. The results showed that Justicidin B attenuated the proliferation induced by platelet-derived growth factor-BB (PDGF-BB) in MCs and blocked cell cycle progression. Likewise, inflammatory factors, including monocyte chemotactic protein 1 (MCP-1) and tumor necrosis factor alpha (TNF-α), in MCs were decreased after treatment with Justicidin B. In addition, Justicidin B exhibited antioxidant activity in PDGF-BB-induced MCs, shown by the decreased production of malondialdehyde and T-AOC, and increased the expression of superoxide dismutase. Besides, Justicidin B suppressed extracellular matrix (ECM) deposition by reducing the protein levels of collagen IV and fibronectin. Furthermore, we found that Justicidin B significantly inhibited activation of the Akt/mammalian target of rapamycin (mTOR) signaling pathway in MCs induced by PDGF-BB, but enhanced the levels of proteins in the nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase 1 (HO-1) pathway. Taken together, Justicidin B prevented PDGF-BB-induced proliferation, inflammation, oxidative stress, and ECM accumulation via regulating the activation of the Nrf2/HO-1 pathway and the Akt/mTOR signaling pathway.

Salivary analysis can be used to assess the severity of caries. Of the known salivary proteins, a paucity of information exists concerning the role of proteinase 3 (PR3), a serine protease of the chymotrypsin family, in dental caries. Whole, unstimulated saliva was collected from children with varying degrees of active caries and tested using a Human Protease Array Kit and an enzyme-linked immunosorbent assay. A significantly decreased concentration of salivary PR3 was noted with increasing severity of dental caries (P

China Fusion Engineering Test Reactor (CFETR) is a tokamak reactor; one design option under the consideration of the China National Integration Design Group employs superconducting magnets. The fusion power is at the range of 50-200 MW and the duty cycle (or burning time) was envisioned as 30%-50%. The plasma current will be 10 MA and the major and minor radii are 5.7 and 1.6 m, respectively. The concept engineering design including the magnet system, vacuum vessel (VV) system, and maintenance method has been carried out in the past years. The toroidal magnetic field strength at R0 is 5 T and the maximum flux swing provided by central solenoid winding will be 160 VS. The main design work, including the electromagnetic analysis of the magnet system, has already been carried out. This paper mainly probes into the VV design and optimization based on three types of maintenance ports. Furthermore, the maintenance method, counted as one of the most important design studies, is presented specifically in this paper. It includes the design of the maintenance ports and the remote handling system design, and so on. The next design stage will mainly include mechanical design, conductor stability, different types of divertor system design, and so on.

This paper studied the health risk of air pollutants SO2, NO2 and PM10 in all the districts and counties of Beijing with exposure-response analysis. Different ecological risk areas based on health risk of air pollutants are recommended in Beijing. The result shows that SO2 and NO2 brought a relatively low health risk, while PM10 brought a relatively high health risk. Air pollutants health risk exists in all the districts and counties of Beijing, and most ecological conservation development zones are low risk areas, while urban functional center zones, urban functional development zones and new urban development zones are mainly in medium risk level.

Based on two different methods, the ecological risk of heavy metals in soils of Beijing was assessed. With the assess method of PaPant ecological risk index, the Rapant ecological risk index of center region is 1.365, which indicates a medium ecological risk, while the other districts of Beijing are in low ecological risk level and the main influential factor is Hg. Based on the assess result by the method of Hakanson potential ecological risk index, The highest value of RI is 141.95, which is still in the low ecological risk level but also close to the lower limit of medium level; the main influential factors is Hg and Cd. Therefore, to focus on the monitoring and management of Cd and Hg in center region is necessary in Beijing.

The soil heavy metal pollution have be paid more attention because the sources of soil heavy metals become multiple and complicated with the accelerated urbanization. According to this research, the order of spatial distribution of soil heavy metal Cu, Zn and Cd pollution in Beijing shows that: the center district > suburb > outer suburb. The soil pollution by Zn is more serious in Beijing and the grade of the soil Zn pollution has reached highest in the central district. By comparison, the soil pollution by Cu and Cd is slight, the soil in central district, Chaoyang, Changping and Miyun has caught light Cu pollution, and only in center district, Chaoyang and Fengtai has caught light Cd pollution.

This paper introduces the composition of pump and valve control system of parallel connection and the output flux of pump and valve is distributed optimally. Then, establish the state-space model of pump and valve system, the system is simulated by Simlink and AMESim software, the simulation results obtained.

In the process of lead-electrolysis, when we take off the cathode lead plate from electrolyte, we should take out the cathode collector bar. Next cathode lead plate will be melted and make into ingot. The cathode collector bar can be used next time. The design is a new kind of appliance of taking out the cathode collector bar. The appliance, which limits the cathode plate horizontally, uses the way of single taking out. It is proved that this way is steady and efficient.