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7. Further epidemiological survey for atovaquone resistant related gene of Babesia gibsoni in Japan during 2015–2018

Author

IGUCHI, Aiko, SOMA, Takehisa, and XUAN, Xuenan

Subjects
allele-specific real-time PCR, drug resistance, Dogs, Japan, M121I variant population, Babesia gibsoni, Babesiosis, Internal Medicine, Animals, Babesia, Dog Diseases, Note, Atovaquone
Abstract

A single-nucleotide polymorphism causing the replacement of methionine with isoleucine (M121I) in cytochrome b of Babesia gibsoni has been reported to reduce the susceptibility to atovaquone (ATV) in B. gibsoni infection. In our previous study, B. gibsoni with M121I was suggested to exist in nature. Thus, further examinations were performed. In total, 105 genomic DNA samples from B. gibsoni-infected dogs were collected from western (98 samples from 15 prefectures) and eastern areas (7 samples from 4 prefectures) in Japan. The M121I variant population was identified using allele-specific real-time PCR: it was then detected in nine samples (8.57%), which was higher than that in the previous study (4.11%). Although there are unclear points, such as the history of ATV usage, careful attention should be given to emerging ATV resistance.

Published
2020

12. Development and evaluation of an enzyme-linked immunosorbent assay based on recombinant TgSRS2 for serodiagnosis of Toxoplasma gondii infection in cats

Author

GAO, Yang, primary, GUO, Huanping, additional, MOUMOUNI, Paul Franck Adjou, additional, LIU, Mingming, additional, LI, Jixu, additional, EFSTRATIOU, Artemis, additional, GALON, Eloiza May, additional, YBANEZ, Rochelle Haidee, additional, MASATANI, Tatsunori, additional, SOMA, Takehisa, additional, and XUAN, Xuenan, additional

Published
2020
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14. Distribution of infectious endogenous retroviruses in mixed-breed and purebred cats

Author

Ngo, Minh Ha, primary, Soma, Takehisa, additional, Youn, Hwa-Young, additional, Endo, Taiji, additional, Makundi, Isaac, additional, Kawasaki, Junna, additional, Miyake, Ariko, additional, Nga, Bui Thi To, additional, Nguyen, Huyen, additional, Arnal, MaríaCruz, additional, Fernández de Luco, Daniel, additional, Deshapriya, R. M. C., additional, Hatoya, Shingo, additional, and Nishigaki, Kazuo, additional

Published
2019
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17. Feline Coronavirus RT-PCR Assays for Feline Infectious Peritonitis Diagnosis

Author

Soma, Takehisa

Subjects
viruses, Diagnosis, RT-PCR, Feline coronavirus, Feline infectious peritonitis, Article
Abstract

Feline infectious peritonitis (FIP) is a highly fatal systemic disease in cats, caused by feline coronavirus (FCoV) infection. FCoV usually has little clinical significance; however, a mutation of this avirulent virus (feline enteric coronavirus) to a virulent type (FIP virus) can lead to FIP incidence. It is difficult to diagnose FIP, since the viruses cannot be distinguished using serological or virological methods. Recently, genetic techniques, such as RT-PCR, have been conducted for FIP diagnosis. In this chapter, the reliability of RT-PCR and procedures used to determine FCoV infection as part of antemortem FIP diagnosis is described.

Published
2015

18. The epidemiological survey for atovaquone resistant related gene of Babesia gibsoni in Japan

Author

Iguchi, Aiko, Soma, Takehisa, Suzuki, Hiroshi, and Xuan, Xuenan

Subjects
allele-specific real-time PCR, drug resistance, M121I variant population, Babesia gibsoni
Abstract

application/pdf, In 73 gDNA samples from Babesia gibsoni-infected dogs, the M121I variant population was measured by using allele-specific real-time PCR. Although the mechanism of atovaquone against B. gibsoni has not been clearly identified, it is reported that the mitochondria cytochrome b gene of the atovaquone-resistant B. gibsoni had a single-nucleotide substitution at nt363 (G to T), which resulted in the substitution of methionine with isoleucine (M121I). In this study, 3/73 samples showed over 5% M121I variant population. Although the M121I variant population is a low percentage, it runs the risk of spreading drug-resistant parasites. It is important to prevent the spread of drug-resistance, so we need to gather information about this at regular intervals.

Published
2016

19. Babesia gibsoni internal transcribed spacer 1 region is highly conserved amongst isolates from dogs across Japan

Author

Liu, Mingming, Cao, Shinuo, Vudriko, Patrick, Suzuki, Hiroshi, Soma, Takehisa, and Xuan, Xuenan

Subjects
Japan, conserved gene, Babesia gibsoni, internal transcribed spacer 1 (ITS1)
Abstract

application/pdf, Babesia gibsoni is a tick-borne apicomplexan parasite of dogs that often causes fever and hemolytic anemia with highly variable clinical outcome. In this study, we sequenced the 254bp Internal Transcribed Spacer 1 region (ITS1) of 54 B. gibsoni isolates from 14 different geographical regions of Japan. The 54 isolates shared high sequence identity with each other and with B. gibsoni isolates reported in GenBank database (97.2–100%). Consistent with previous reports, phylogenetic analysis showed that B. gibsoni isolates from Japan formed the same clade with those from U.S.A., Australia, India and Taiwan. Our finding indicates that B. gibsoni ITS1 region is highly conserved among isolates from dogs in Japan, making it a useful genetic marker for molecular epidemiology of the parasite.

Published
2016

23. Expression of truncated Babesia gibsoni thrombospondin-related adhesive proteins in Escherichia coli and evaluation of their diagnostic potential by enzyme-linked immunosorbent assay

Author

Sandagdorj, Narantsatsral, Goo, Youn-Kyoung, Terkawi, Mohamad Alaa, Soma, Takehisa, Luo, Yuzi, Li, Yan, Cao, Shinuo, Aboge, Gabriel Oluga, Nishikawa, Yoshifumi, Badgar, Battsetseg, and Xuan, Xuenan

Subjects
diagnosis, Babesia gibsoni, enzyme-linked immunosorbent assay (ELISA), thrombospondin-related adhesive protein (TRAP)
Abstract

application/pdf, Among the previously established enzyme-linked immunosorbent assays (ELISAs), an ELISA using the full length of recombinant thrombospondin-related adhesive protein of Babesia gibsoni (rBgTRAPf) is considered as the most sensitive diagnostic method for the detection of antibody to B. gibsoni in dogs. However, the expression of rBgTRAPf in high concentration is poor and thus limits its usefulness as diagnostic antigen. To improve its expression level, we have truncated BgTRAPf into two fragments having either N- or C-terminus (BgTRAPn or BgTRAPc). The expression of BgTRAPc protein in Escherichia coli yielded adequate recombinant protein. The specificity and sensitivity of ELISAs with the truncated proteins were determined using B. gibsoni-experimentally infected dog sera and specific pathogen-free (SPF) dog sera. A total of 254 field dog sera were examined by the ELISA with rBgTRAPn, rBgTRAPc and rBgTRAPf as well as by indirect fluorescent antibody test (IFAT). The specificity of rBgTRAPc was highest (97.15%) and its kappa value was more (0.8003) than rBgTRAPn (0.7083). With sufficient level of expression as well as higher specificity and reliable sensitivity, rBgTRAPc appears to be a potential candidate antigen for serodiagnosis of B. gibsoni infection in dogs.

Published
2011

25. Prevalence of microorganisms associated with feline gingivostomatitis.

Author

Nakanishi, Hitoshi, Furuya, Masaru, Soma, Takehisa, Hayashiuchi, Yoshiki, Yoshiuchi, Ryusaku, Matsubayashi, Makoto, Tani, Hiroyuki, and Sasai, Kazumi

Abstract

Objectives Feline gingivostomatitis (FGS) is a painful chronic inflammatory disease of the oral cavity. The purpose of this study was to examine the frequency of detection of certain common feline bacteria and viruses to determine any potential associations with FGS. Methods A multicentre case-control study design was conducted. In total, 72 control cats and 32 cats with FGS were included in the study. Oral swabs were cultured for bacterial identification and a PCR assay was carried out to examine the infection of feline calicivirus (FCV), feline herpesvirus-1 (FHV-1), Chlamydia felis, Mycoplasma felis and Bordetella bronchiseptica. Results There was a significant difference in age distribution between the control and the FGS group. Based on a PCR assay, the positive rate of FCV was significantly higher in FGS cats than control animals. For other infectious pathogens, including FHV-1, C felis and M felis, there was no significant difference. Bacterial culture of oral swabs revealed that Pasteurella multocida was most frequently detected, but the detection rate was significantly lower in FGS cats. In FGS cats, the incidence of Enterococcus faecalis and anaerobic bacteria were more frequently isolated than in control cats. Conclusions and relevance This study indicates that the positive rate of FCV was significantly higher in cats with FGS, and the microflora of the oral cavity of cats with FGS might be disrupted, although additional studies are required to compare the oral microbiome in cats of a variety of ages. [ABSTRACT FROM AUTHOR]

Published
2019
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39. Expression of truncated Babesia gibsoni thrombospondin-related adhesive proteins in Escherichia coli and evaluation of their diagnostic potential by enzyme-linked immunosorbent assay

Author

Narantsatsral, S., primary, Goo, Youn-Kyoung, additional, Battsetseg, B., additional, Myagmarsuren, P., additional, Terkawi, Mohamad Alaa, additional, Soma, Takehisa, additional, Luo, Yuzi, additional, Li, Yan, additional, Cao, Shinuo, additional, Yu, Longzheng, additional, Kamyingkird, Ketsarin, additional, Aboge, Gabriel Oluga, additional, Nishikawa, Yoshifumi, additional, and Xuan, Xuenan, additional

Published
2011
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44. Genetic variations of four immunodominant antigens of Babesia gibsoniisolated from dogs in southwest Japan

Author

Liu, Mingming, Cao, Shinuo, Zhou, Mo, Wang, Guanbo, Jirapattharasate, Charoonluk, Adjou Moumouni, Paul Franck, Iguchi, Aiko, Vudriko, Patrick, Suzuki, Hiroshi, Soma, Takehisa, and Xuan, Xuenan

Abstract

Babesia gibsoniis a tick-borne apicomplexan parasite of dogs that often causes fever and hemolytic anemia. Previous reports have shown that the apical membrane antigen 1 (BgAMA1), the 50kDa surface antigen (BgP50), the secreted antigen 1 (BgSA1), and the thrombospondin-related adhesive protein (BgTRAP) are promising diagnostic antigens and vaccine candidates against B. gibsoni. In the present study, we investigated the genetic polymorphisms and natural selection of these four genes of B. gibsoniisolated from dogs in southwest Japan. The prediction B-cell epitopes showed high antigenic score in the insert and indel regions of BgSA1 and BgTRAP. Sequence analyses have revealed that BgAMA1 had the highest nucleotide diversity, followed by BgP50, BgSA1 and BgTRAP. Meanwhile, the Tajima's D value test suggested balancing selection for BgAMA1 and BgP50. However, BgSA1 and BgTRAP have purifying selection making them potential vaccine candidate and diagnostic antigens since they are highly conserved. Our findings provide the genetic basis for designing and testing the efficacy of diagnostic antigens as well as vaccine candidates against B. gibsoni.

Published
2016
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