28 results on '"Solanas AM"'
Search Results
2. Coupling chemical oxidation and biostimulation: Effects on the natural attenuation capacity and resilience of the native microbial community in alkylbenzene-polluted soil.
- Author
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Martínez-Pascual E, Grotenhuis T, Solanas AM, and Viñas M
- Subjects
- Bacteria genetics, Bacteria metabolism, Biodegradation, Environmental, Carbon Dioxide metabolism, DNA, Bacterial genetics, Hydrogen Peroxide chemistry, Iron chemistry, Oxidation-Reduction, Oxygen metabolism, Potassium Permanganate chemistry, RNA, Ribosomal, 16S genetics, Soil Microbiology, Water Microbiology, Benzene Derivatives chemistry, Benzene Derivatives metabolism, Soil Pollutants chemistry, Soil Pollutants metabolism, Water Pollutants, Chemical chemistry, Water Pollutants, Chemical metabolism
- Abstract
Coupling chemical oxidation with bioremediation could be a cost-effective system to cope with soil and groundwater pollution. However, the effects of chemical oxidation on autochthonous microbial communities are scarcely known. A detailed analysis that considers both the efficiency of the two technologies and the response of the microbial communities was performed on a linear alkylbenzene-polluted soil and groundwater samples. The impacts of a modified Fenton's reaction (MFR) at various dosages and of permanganate on the microbiota over 4 weeks were assessed. The permanganate and MFR negatively affected microbial abundance and activity. However, the resilience of certain microbial populations was observed, with a final increase in potential hydrocarbon-degrading populations as determined by both the alkB gene abundance and the predominance of well-known hydrocarbon-degrading phylotypes such as Rhodococcus, Ochrobactrum, Acinetobacter and Cupriavidus genera as determined by 16S rRNA-based DGGE fingerprinting. The assessment of the chemical oxidant impact on autochthonous microbiota should be considered for the optimization of coupled field remediation technologies., (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Published
- 2015
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3. Pyrosequencing reveals the effect of mobilizing agents and lignocellulosic substrate amendment on microbial community composition in a real industrial PAH-polluted soil.
- Author
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Lladó S, Covino S, Solanas AM, Petruccioli M, D'annibale A, and Viñas M
- Subjects
- Bacteria classification, Biodegradation, Environmental, Biodiversity, DNA, Ribosomal Spacer genetics, Denaturing Gradient Gel Electrophoresis, Fungi classification, Industry, RNA, Ribosomal, 16S genetics, Sequence Analysis, DNA, Soil chemistry, Surface-Active Agents chemistry, Creosote analysis, Polycyclic Aromatic Hydrocarbons analysis, Soil Microbiology, Soil Pollutants analysis
- Abstract
Bacterial and fungal biodiversity throughout different biostimulation and bioaugmentation treatments applied to an industrial creosote-polluted soil were analyzed by means of polyphasic approach in order to gain insight into the microbial community structure and dynamics. Pyrosequencing data obtained from initial creosote polluted soil (after a biopiling step) revealed that Alpha and Gammaproteobacteria were the most abundant bacterial groups, whereas Fusarium and Scedosporium were the main fungal genera in the contaminated soil. At the end of 60-days laboratory scale bioremediation assays, pyrosequencing and DGGE data showed that (i) major bacterial community shifts were caused by the type of mobilizing agent added to the soil and, to a lesser extent, by the addition of lignocellulosic substrate; and (ii) the presence of the non-ionic surfactant (Brij 30) hampered the proliferation of Actinobacteria (Mycobacteriaceae) and Bacteroidetes (Chitinophagaceae) and, in the absence of lignocellulosic substrate, also impeded polycyclic aromatic hydrocarbons (PAHs) degradation. The results show the importance of implementing bioremediation experiments combined with microbiome assessment to gain insight on the effect of crucial parameters (e.g. use of additives) over the potential functions of complex microbial communities harbored in polluted soils, essential for bioremediation success., (Copyright © 2014 Elsevier B.V. All rights reserved.)
- Published
- 2015
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4. Comparative assessment of bioremediation approaches to highly recalcitrant PAH degradation in a real industrial polluted soil.
- Author
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Lladó S, Covino S, Solanas AM, Viñas M, Petruccioli M, and D'annibale A
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- Bacteria drug effects, Bacteria metabolism, Biodegradation, Environmental, Industrial Waste, Lignin pharmacology, Manganese pharmacology, Polidocanol, Polyethylene Glycols pharmacology, RNA, Bacterial genetics, RNA, Fungal genetics, RNA, Ribosomal, 16S genetics, Soybean Oil pharmacology, Surface-Active Agents pharmacology, Lentinula metabolism, Polycyclic Aromatic Hydrocarbons metabolism, Soil Microbiology, Soil Pollutants metabolism, Trametes metabolism
- Abstract
High recalcitrant characteristics and low bioavailability rates due to aging processes can hinder high molecular weight polycyclic aromatic hydrocarbons (HMW-PAHs) bioremediation in real industrial polluted soils. With the aim of reducing the residual fraction of total petroleum hydrocarbons (TPH) and (HMW-PAHs) in creosote-contaminated soil remaining after a 180-d treatment in a pilot-scale biopile, either biostimulation (BS) of indigenous microbial populations with a lignocellulosic substrate (LS) or fungal bioaugmentation with two strains of white-rot fungi (WRF) (i.e., Trametes versicolor and Lentinus tigrinus) were comparatively tested. The impact of bivalent manganese ions and two mobilizing agents (MAs) (i.e., Soybean Oil and Brij 30) on the degradation performances of biostimulated and bioaugmented microcosms was also compared. The results reveal soil colonization by both WRF strains was clearly hampered by an active native soil microbiota. In fact, a proper enhancement of native microbiota by means of LS amendment promoted the highest biodegradation of HMW-PAHs, even of those with five aromatic rings after 60 days of treatment, but HMW-PAH-degrading bacteria were specifically inhibited when non-ionic surfactant Brij 30 was amended. Effects of bioaugmentation and other additives such as non-ionic surfactants on the degrading capability of autochthonous soil microbiota should be evaluated in polluted soils before scaling up the remediation process at field scale., (Copyright © 2013 Elsevier B.V. All rights reserved.)
- Published
- 2013
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5. A diversified approach to evaluate biostimulation and bioaugmentation strategies for heavy-oil-contaminated soil.
- Author
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Lladó S, Solanas AM, de Lapuente J, Borràs M, and Viñas M
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- Biodegradation, Environmental, Glycolipids metabolism, Microbial Consortia physiology, Soil Microbiology, Surface-Active Agents metabolism, Toxicity Tests, Acute methods, Petroleum metabolism, Petroleum microbiology, Soil Pollutants metabolism
- Abstract
A diversified approach involving chemical, microbiological and ecotoxicity assessment of soil polluted by heavy mineral oil was adopted, in order to improve our understanding of the biodegradability of pollutants, microbial community dynamics and ecotoxicological effects of various bioremediation strategies. With the aim of improving hydrocarbon degradation, the following bioremediation treatments were assayed: i) addition of inorganic nutrients; ii) addition of the rhamnolipid-based biosurfactant M(AT10); iii) inoculation of an aliphatic hydrocarbon-degrading microbial consortium (TD); and iv) inoculation of a known hydrocarbon-degrading white-rot fungus strain of Trametes versicolor. After 200 days, all the bioremediation assays achieved between 30% and 50% total petroleum hydrocarbon (TPH) biodegradation, with the T. versicolor inoculation degrading it the most. Biostimulation and T. versicolor inoculation promoted the Brevundimonas genus concurrently with other α-proteobacteria, β-proteobacteria and Cytophaga-Flexibacter-Bacteroides (CFB) as well as Actinobacteria groups. However, T. versicolor inoculation, which produced the highest hydrocarbon degradation in soil, also promoted autochthonous Gram-positive bacterial groups, such as Firmicutes and Actinobacteria. An acute toxicity test using Eisenia fetida confirmed the improvement in the quality of the soil after all biostimulation and bioaugmentation strategies., (Copyright © 2012 Elsevier B.V. All rights reserved.)
- Published
- 2012
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6. Polyphasic approach for assessing changes in an autochthonous marine bacterial community in the presence of Prestige fuel oil and its biodegradation potential.
- Author
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Jiménez N, Viñas M, Guiu-Aragonés C, Bayona JM, Albaigés J, and Solanas AM
- Subjects
- Alphaproteobacteria isolation & purification, Alphaproteobacteria metabolism, Aquatic Organisms microbiology, Bacteria classification, Bacteria genetics, Bacteria metabolism, Bacterial Typing Techniques, Base Sequence, Biodegradation, Environmental, Gammaproteobacteria isolation & purification, Gammaproteobacteria metabolism, Microbial Consortia, Molecular Sequence Data, RNA, Ribosomal, 16S genetics, Seawater microbiology, Sequence Analysis, DNA, Fuel Oils microbiology, Hydrocarbons metabolism
- Abstract
A laboratory experiment was conducted to identify key hydrocarbon degraders from a marine oil spill sample (Prestige fuel oil), to ascertain their role in the degradation of different hydrocarbons, and to assess their biodegradation potential for this complex heavy oil. After a 17-month enrichment in weathered fuel, the bacterial community, initially consisting mainly of Methylophaga species, underwent a major selective pressure in favor of obligate hydrocarbonoclastic microorganisms, such as Alcanivorax and Marinobacter spp. and other hydrocarbon-degrading taxa (Thalassospira and Alcaligenes), and showed strong biodegradation potential. This ranged from >99% for all low- and medium-molecular-weight alkanes (C(15)-C(27)) and polycyclic aromatic hydrocarbons (C(0)- to C(2)- naphthalene, anthracene, phenanthrene, dibenzothiophene, and carbazole), to 75-98% for higher molecular-weight alkanes (C(28)-C(40)) and to 55-80% for the C(3) derivatives of tricyclic and tetracyclic polycyclic aromatic hydrocarbons (PAHs) (e.g., C(3)-chrysenes), in 60 days. The numbers of total heterotrophs and of n-alkane-, aliphatic-, and PAH degraders, as well as the structures of these populations, were monitored throughout the biodegradation process. The salinity of the counting medium affects the counts of PAH degraders, while the carbon source (n-hexadecane vs. a mixture of aliphatic hydrocarbons) is a key factor when counting aliphatic degraders. These limitations notwithstanding, some bacterial genera associated with hydrocarbon degradation (mainly belonging to α- and γ-Proteobacteria, including the hydrocarbonoclastic Alcanivorax and Marinobacter) were identified. We conclude that Thalassospira and Roseobacter contribute to the degradation of aliphatic hydrocarbons, whereas Mesorhizobium and Muricauda participate in the degradation of PAHs.
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- 2011
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7. Chemical and microbial community analysis during aerobic biostimulation assays of non-sulfonated alkyl-benzene-contaminated groundwater.
- Author
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Martínez-Pascual E, Jiménez N, Vidal-Gavilan G, Viñas M, and Solanas AM
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- Aerobiosis, Alkanesulfonic Acids chemistry, Biodegradation, Environmental, Colony Count, Microbial, Hydrocarbons chemistry, Hydrocarbons metabolism, Petrolatum adverse effects, Petrolatum metabolism, Water Pollution, Chemical, Bacteria, Aerobic metabolism, Water Microbiology, Water Pollutants, Chemical analysis
- Abstract
A chemical and microbial characterization of lab-scale biostimulation assays with groundwater samples taken from an industrial site in which the aquifer had been contaminated by linear non-sulfonate alkyl benzenes (LABs) was carried out for further field-scale bioremediation purposes. Two lab-scale biodegradability assays were performed, one with a previously obtained gas-oil-degrading consortium and another with the native groundwater flora. Results for the characterization of the groundwater microbial population of the site revealed the presence of an important LAB-degrading microbial population with a strong degrading capacity. Among the microorganisms identified at the site, the detection of Parvibaculum lavamentivorans, which have been described in other studies as alkyl benzene sulfonates degraders, is worth mentioning. Incubation of P. lavamentivorans DSMZ13023 with LABs as reported in this study shows for the first time the metabolic capacity of this strain to degrade such compounds. Results from the biodegradation assays in this study showed that the indigenous microbial population had a higher degrading capacity than the gas-oil-degrading consortium, indicating the strong ability of the native community to adapt to the presence of LABs. The addition of inorganic nutrients significantly improved the aerobic biodegradation rate, achieving levels of biodegradation close to 90%. The results of this study show the potential effectiveness of oxygen and nutrients as in situ biostimulation agents as well as the existence of a complex microbial community that encompasses well-known hydrocarbon- and LAS-degrading microbial populations in the aquifer studied.
- Published
- 2010
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8. Microbial populations related to PAH biodegradation in an aged biostimulated creosote-contaminated soil.
- Author
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Lladó S, Jiménez N, Viñas M, and Solanas AM
- Subjects
- Bacteroidetes metabolism, Base Sequence, Creosote metabolism, Molecular Sequence Data, Mycobacterium metabolism, Pseudomonas metabolism, Soil Pollutants metabolism, Benz(a)Anthracenes metabolism, Biodegradation, Environmental, Chrysenes metabolism, Polycyclic Aromatic Hydrocarbons metabolism, Soil Microbiology
- Abstract
A previous bioremediation survey on a creosote-contaminated soil showed that aeration and optimal humidity promoted depletion of three-ringed polycyclic aromatic hydrocarbons (PAHs), but residual concentrations of four-ringed benzo(a)anthracene (B(a)A) and chrysene (Chry) remained. In order to explain the lack of further degradation of heavier PAHs such as four-ringed PAHs and to analyze the microbial population responsible for PAH biodegradation, a chemical and microbial molecular approach was used. Using a slurry incubation strategy, soil in liquid mineral medium with and without additional B(a)A and Chry was found to contain a powerful PAH-degrading microbial community that eliminated 89% and 53% of the added B(a)A and Chry, respectively. It is hypothesized that the lack of PAH bioavailability hampered their further biodegradation in the unspiked soil. According to the results of the culture-dependent and independent techniques Mycobacterium parmense, Pseudomonas mexicana, and Sphingobacterials group could control B(a)A and Chry degradation in combination with several microorganisms with secondary metabolic activity.
- Published
- 2009
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9. Bacterial communities from shoreline environments (costa da morte, northwestern Spain) affected by the prestige oil spill.
- Author
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Alonso-Gutiérrez J, Figueras A, Albaigés J, Jiménez N, Viñas M, Solanas AM, and Novoa B
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- Alkenes metabolism, Bacteria genetics, Biodegradation, Environmental, Cluster Analysis, DNA Fingerprinting, DNA, Bacterial chemistry, DNA, Bacterial genetics, DNA, Ribosomal chemistry, DNA, Ribosomal genetics, Electrophoresis, Polyacrylamide Gel, Hydrocarbons, Aromatic metabolism, Mineral Oil, Molecular Sequence Data, Nucleic Acid Denaturation, Phylogeny, RNA, Ribosomal, 16S genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Spain, Bacteria classification, Bacteria isolation & purification, Biodiversity, Geologic Sediments microbiology, Water Pollution, Chemical
- Abstract
The bacterial communities in two different shoreline matrices, rocks and sand, from the Costa da Morte, northwestern Spain, were investigated 12 months after being affected by the Prestige oil spill. Culture-based and culture-independent approaches were used to compare the bacterial diversity present in these environments with that at a nonoiled site. A long-term effect of fuel on the microbial communities in the oiled sand and rock was suggested by the higher proportion of alkane and polyaromatic hydrocarbon (PAH) degraders and the differences in denaturing gradient gel electrophoresis patterns compared with those of the reference site. Members of the classes Alphaproteobacteria and Actinobacteria were the prevailing groups of bacteria detected in both matrices, although the sand bacterial community exhibited higher species richness than the rock bacterial community did. Culture-dependent and -independent approaches suggested that the genus Rhodococcus could play a key role in the in situ degradation of the alkane fraction of the Prestige fuel together with other members of the suborder Corynebacterineae. Moreover, other members of this suborder, such as Mycobacterium spp., together with Sphingomonadaceae bacteria (mainly Lutibacterium anuloederans), were related as well to the degradation of the aromatic fraction of the Prestige fuel. The multiapproach methodology applied in the present study allowed us to assess the complexity of autochthonous microbial communities related to the degradation of heavy fuel from the Prestige and to isolate some of their components for a further physiological study. Since several Corynebacterineae members related to the degradation of alkanes and PAHs were frequently detected in this and other supralittoral environments affected by the Prestige oil spill along the northwestern Spanish coast, the addition of mycolic acids to bioremediation amendments is proposed to favor the presence of these degraders in long-term fuel pollution-affected areas with similar characteristics.
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- 2009
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10. The Prestige oil spill: bacterial community dynamics during a field biostimulation assay.
- Author
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Jiménez N, Viñas M, Bayona JM, Albaiges J, and Solanas AM
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- Alphaproteobacteria genetics, Alphaproteobacteria isolation & purification, Biological Assay, DNA, Bacterial genetics, DNA, Bacterial isolation & purification, Environmental Monitoring methods, Polycyclic Aromatic Hydrocarbons analysis, RNA, Ribosomal, 16S analysis, Spain, Water Pollutants, Chemical, Alphaproteobacteria metabolism, Biodegradation, Environmental, Fertilizers, Fuel Oils, Polycyclic Aromatic Hydrocarbons metabolism
- Abstract
A field bioremediation assay using the oleophilic fertilizer S200 was carried out 12 months after the Prestige heavy fuel-oil spill on a beach on the Cantabrian coast (north Spain). This assay showed that S200-enhanced oil degradation, particularly of high-molecular-weight n-alkanes and alkylated PAHs, suggesting an increase in the microbial bioavailability of these compounds. The bacterial community structure was determined by cultivation-independent analysis of polymerase chain reaction-amplified 16S rDNA by denaturing gradient gel electrophoresis. Bacterial community was mainly composed of alpha-Proteobacteria (Rhodobacteriaceae and Sphingomonadaceae). Representatives of gamma-Proteobacteria (Chromatiales, Moraxellaceae, and Halomonadaceae), Bacteroidetes (Flavobacteriaceae), and Actinobacteria group (Nocardiaceae and Corynebacteriaceae) were also found. The addition of the fertilizer led to the appearance of the bacterium Mesonia algae in the early stages, with a narrow range of growth substrates, which has been associated with the common alga Achrosiphonia sonderi. The presence of Mesonia algae may be attributable to the response of the microbial community to the addition of N and P rather than indicating a role in the biodegradation process. The Rhodococcus group appeared in both assay plots, especially at the end of the experiment. It was also found at another site on the Galician coast that had been affected by the same spill. This genus has been associated with the degradation of n-alkanes up to C(36). Taking into account the high content of heavy alkanes in the Prestige fuel, these microorganisms could play a significant role in the degradation of such fuel. A similar bacterial community structure was observed at another site that showed a similar degree of fuel weathering.
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- 2007
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11. Bioavailability assessment and environmental fate of polycyclic aromatic hydrocarbons in biostimulated creosote-contaminated soil.
- Author
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Sabaté J, Viñas M, and Solanas AM
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- Adsorption, Bacteria drug effects, Bacteria metabolism, Biodegradation, Environmental, Cyclodextrins chemistry, Polycyclic Aromatic Hydrocarbons chemistry, Polycyclic Aromatic Hydrocarbons metabolism, Polycyclic Aromatic Hydrocarbons toxicity, Soil Pollutants metabolism, Soil Pollutants toxicity, Creosote, Polycyclic Aromatic Hydrocarbons analysis, Soil Microbiology, Soil Pollutants analysis
- Abstract
When hydrocarbon-contaminated soil is subjected to bioremediation technology, hydrocarbon depletion is typically marked by an initially rapid reduction rate. This rate decreases over time and frequently a residual concentration remains in the soil. This kinetic has been attributed primarily to the enrichment of more recalcitrant fractions, as well as to the lack of resting hydrocarbon bioavailability. Thus, at the end of the bioremediation process, a part of the residual hydrocarbon soil concentration represents the non-bioavailable fraction, which is difficult to degrade by microbial populations and which poses a minor hazard. Therefore, determination of the bioavailable fraction in a bioremediation project represents both an estimation of the maximum level of achievable biodegradation, as well as an additional indication of the environmental health hazard. In the present study, aged creosote-contaminated soil was subjected to biostimulation processes, and the bioavailable fraction for several target polycyclic aromatic hydrocarbons (PAHs) was calculated using a mild extraction with cyclodextrines. The amount of PAH extracted corresponded to the desorbing fraction and can be regarded as the bioavailable fraction. The non-desorbing fraction data obtained from this procedure were compared to the remaining PAH concentrations following bioremediation treatment of soil microcosms. These results permitted the establishment of a theoretical biodegradation limit based on the desorbing fraction. In addition, neither accumulation of intermediate metabolites, nor the formation of bound-residues or reduced acute toxicity was observed.
- Published
- 2006
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12. The Prestige oil spill. 2. Enhanced biodegradation of a heavy fuel oil under field conditions by the use of an oleophilic fertilizer.
- Author
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Jiménez N, Viñas M, Sabaté J, Díez S, Bayona JM, Solanas AM, and Albaiges J
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- Accidents, Biodegradation, Environmental, Environmental Pollutants, Hydrocarbons analysis, Hydrocarbons metabolism, Spain, Fertilizers, Fuel Oils
- Abstract
A field bioremediation assay using the oleophilic fertilizer S200 was carried out 10 months after the Prestige heavy fuel-oil spill on a beach of the Cantabrian coast (North Spain). The field survey showed that S200 significantly enhanced the biodegradation rate, particularly of high molecular weight n-alkanes, alkylcyclohexanes, and benzenes, and alkylated PAHs, paralleling the results previously found in vitro. The most significant molecular bioremediation indicators were the depletion of diasteranes and C-27 sterane components. Enhanced isomeric selectivity was also observed within the C1-phenanthrenes and dibenzothiophenes. Through the analysis of some target aliphatic and aromatic hydrocarbons a number of chemical indicators for assessing the efficiency of field bioremediation as well as identifying the source of highly weathered samples collected in the area after the spill are defined.
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- 2006
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13. Bacterial community dynamics and polycyclic aromatic hydrocarbon degradation during bioremediation of heavily creosote-contaminated soil.
- Author
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Viñas M, Sabaté J, Espuny MJ, and Solanas AM
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- Bacteria classification, Bacteria genetics, Bacteria metabolism, Biodegradation, Environmental, Colony Count, Microbial, DNA, Bacterial analysis, Electrophoresis methods, Molecular Sequence Data, Petroleum metabolism, Phylogeny, Polymerase Chain Reaction, RNA, Ribosomal, 16S genetics, Bacteria growth & development, Creosote metabolism, Ecosystem, Polycyclic Aromatic Hydrocarbons metabolism, Soil Microbiology, Soil Pollutants metabolism
- Abstract
Bacterial community dynamics and biodegradation processes were examined in a highly creosote-contaminated soil undergoing a range of laboratory-based bioremediation treatments. The dynamics of the eubacterial community, the number of heterotrophs and polycyclic aromatic hydrocarbon (PAH) degraders, and the total petroleum hydrocarbon (TPH) and PAH concentrations were monitored during the bioremediation process. TPH and PAHs were significantly degraded in all treatments (72 to 79% and 83 to 87%, respectively), and the biodegradation values were higher when nutrients were not added, especially for benzo(a)anthracene and chrysene. The moisture content and aeration were determined to be the key factors associated with PAH bioremediation. Neither biosurfactant addition, bioaugmentation, nor ferric octate addition led to differences in PAH or TPH biodegradation compared to biodegradation with nutrient treatment. All treatments resulted in a high first-order degradation rate during the first 45 days, which was markedly reduced after 90 days. A sharp increase in the size of the heterotrophic and PAH-degrading microbial populations was observed, which coincided with the highest rates of TPH and PAH biodegradation. At the end of the incubation period, PAH degraders were more prevalent in samples to which nutrients had not been added. Denaturing gradient gel electrophoresis analysis and principal-component analysis confirmed that there was a remarkable shift in the composition of the bacterial community due to both the biodegradation process and the addition of nutrients. At early stages of biodegradation, the alpha-Proteobacteria group (genera Sphingomonas and Azospirillum) was the dominant group in all treatments. At later stages, the gamma-Proteobacteria group (genus Xanthomonas), the alpha-Proteobacteria group (genus Sphingomonas), and the Cytophaga-Flexibacter-Bacteroides group (Bacteroidetes) were the dominant groups in the nonnutrient treatment, while the gamma-Proteobacteria group (genus Xathomonas), the beta-Proteobacteria group (genera Alcaligenes and Achromobacter), and the alpha-Proteobacteria group (genus Sphingomonas) were the dominant groups in the nutrient treatment. This study shows that specific bacterial phylotypes are associated both with different phases of PAH degradation and with nutrient addition in a preadapted PAH-contaminated soil. Our findings also suggest that there are complex interactions between bacterial species and medium conditions that influence the biodegradation capacity of the microbial communities involved in bioremediation processes.
- Published
- 2005
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14. Culture-dependent and -independent approaches establish the complexity of a PAH-degrading microbial consortium.
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Viñas M, Sabaté J, Guasp C, Lalucat J, and Solanas AM
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- Ascomycota classification, Ascomycota genetics, Ascomycota isolation & purification, Ascomycota metabolism, Bacteria genetics, Bacteria metabolism, Bacteroides classification, Bacteroides genetics, Bacteroides isolation & purification, Bacteroides metabolism, Biodegradation, Environmental, Cytophaga classification, Cytophaga genetics, Cytophaga isolation & purification, Cytophaga metabolism, DNA Fingerprinting, DNA, Bacterial chemistry, DNA, Bacterial genetics, DNA, Fungal chemistry, DNA, Fungal genetics, DNA, Ribosomal chemistry, DNA, Ribosomal genetics, Electrophoresis, Polyacrylamide Gel, Flexibacter classification, Flexibacter genetics, Flexibacter isolation & purification, Flexibacter metabolism, Fungi genetics, Fungi metabolism, Molecular Sequence Data, Phylogeny, Proteobacteria classification, Proteobacteria genetics, Proteobacteria isolation & purification, Proteobacteria metabolism, RNA, Ribosomal, 16S genetics, RNA, Ribosomal, 18S chemistry, RNA, Ribosomal, 18S genetics, Sequence Homology, Nucleic Acid, Soil Microbiology, Bacteria classification, Bacteria isolation & purification, Biodiversity, Fungi classification, Fungi isolation & purification, Polycyclic Aromatic Hydrocarbons metabolism
- Abstract
A microbial consortium (AM) obtained by sequential enrichment in liquid culture with a polycyclic aromatic hydrocarbon (PAH) mixture of three- and four-ringed PAHs as a sole source of carbon and energy was examined using a triple-approach method based on various cultivation strategies, denaturing gradient gel electrophoresis (DGGE), and the screening of 16S and 18S rRNA gene clone libraries. Eleven different sequences by culture-dependent techniques and seven by both DGGE and clone libraries were obtained. The comparison of three variable regions (V3-V5) of the 16S rRNA gene between the sequences obtained yielded 19 different microbial components. Proteobacteria were the dominant group, representing 83% of the total, while the Cytophaga-Flexibacter-Bacteroides group (CFB) was 11% and the Ascomycota fungi 6%. Beta-proteobacteria were predominant in the DGGE and clone library methods, whereas they were a minority in culturable strains. The highest diversity and number of noncoincident sequences were achieved by the cultivation method that showed members of the alpha-, beta-, and gamma-Proteobacteria; CFB bacterial group; and Ascomycota fungi. Only six of the 11 strains isolated showed PAH-degrading capability. The bacterial strain (AMS7) and the fungal strain (AMF1), which were similar to Sphingomonas sp. and Fusarium sp., respectively, achieved the greatest PAH depletion. The results indicate that polyphasic assessment is necessary for a proper understanding of the composition of a microbial consortium.
- Published
- 2005
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15. The prestige oil spill. I. Biodegradation of a heavy fuel oil under simulated conditions.
- Author
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Díez S, Sabatté J, Viñas M, Bayona JM, Solanas AM, and Albaigés J
- Subjects
- Alkanes chemistry, Benzene chemistry, Chromatography, Cyclohexanes chemistry, Environmental Monitoring methods, Environmental Pollution, Fertilizers, Gas Chromatography-Mass Spectrometry, Hydrocarbons chemistry, Ions, Models, Chemical, Naphthalenes chemistry, Oils, Phenanthrenes chemistry, Polycyclic Aromatic Hydrocarbons chemistry, Terpenes chemistry, Time Factors, Triterpenes chemistry, Biodegradation, Environmental, Fuel Oils, Water Pollutants, Chemical
- Abstract
In vitro biodegradation of the Prestige heavy fuel oil has been carried out using two microbial consortia obtained by enrichment in different substrates to simulate its environmental fate and potential utility for bioremediation. Different conditions, such as incubation time (i.e., 20 or 40 d), oil weathering, and addition of an oleophilic fertilizer (S200), were evaluated. Weathering slowed down the degradation of the fuel oil, probably because of the loss of lower and more labile components, but the addition of S200 enhanced significantly the extension of the biodegradation. n-Alkanes, alkylcyclohexanes, alkylbenzenes, and the two- to three-ring polycyclic aromatic hydrocarbons (PAHs) were degraded in 20 or 40 d of incubation of the original oil, whereas the biodegradation efficiency decreased for higher PAHs and with the increase of alkylation. Molecular markers were degraded according to the following sequence: Acyclic isoprenoids > diasteranes > C27-steranes > betabeta-steranes > homohopanes > monoaromatic steranes > triaromatic steranes. Isomeric selectivity was observed within the C1- and C2-phenanthrenes, dibenzothiophenes, pyrenes, and chrysenes, providing source and weathering indices for the characterization of the heavy oil spill. Acyclic isoprenoids, C27-steranes, C1- and C2-naphthalenes, phenanthrenes, and dibenzothiophenes were degraded completely when S200 was used. The ratios of the C2- and C3-alkyl homologues of fluoranthene/pyrene and chrysene/benzo[a]anthracene are proposed as source ratios in moderately degraded oils. The 4-methylpyrene and 3-methylchrysene were refractory enough to serve as conserved internal markers in assessing the degradation of the aromatic fraction in a manner similar to that of hopane, as used for the aliphatic fraction.
- Published
- 2005
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16. Enhanced biodegradation of Casablanca crude oil by a microbial consortium in presence of a rhamnolipid produced by Pseudomonas aeruginosa AT10.
- Author
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Abalos A, Viñas M, Sabaté J, Manresa MA, and Solanas AM
- Subjects
- Biodegradation, Environmental, Culture Media, Emulsions metabolism, Glycolipids chemistry, Glycolipids metabolism, Petroleum analysis, Polycyclic Aromatic Hydrocarbons metabolism, Soil Microbiology, Soil Pollutants metabolism, Terpenes metabolism, Time Factors, Glycolipids biosynthesis, Petroleum metabolism, Pseudomonas aeruginosa metabolism
- Abstract
The biodegradation of oil products in the environment is often limited by their low water solubility and dissolution rate. Rhamnolipids produced by Pseudomonas aeruginosa AT10 were investigated for their potential to enhance bioavailability and hence the biodegradation of crude oil by a microbial consortium in liquid medium. The characterization of the rhamnolipids produced by strain AT10 showed the effectiveness of emulsification of complex mixtures. The addition of rhamnolipids accelerates the biodegradation of total petroleum hydrocarbons from 32% to 61% at 10 days of incubation. Nevertheless, the enhancement of biosurfactant addition was more noticeable in the case of the group of isoprenoids from the aliphatic fraction and the alkylated polycyclic aromatic hydrocarbons (PHAS) from the aromatic fraction. The biodegradation of some targeted isoprenoids increased from 16% to 70% and for some alkylated PAHs from 9% to 44%.
- Published
- 2004
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17. Isolation and taxonomic and catabolic characterization of a 3,6-dimethylphenanthrene-utilizing strain of Sphingomonas sp.
- Author
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Sabaté J, Viñas M, Bayona JM, and Solanas AM
- Subjects
- Alkanes metabolism, Biodegradation, Environmental, Creosote chemistry, Creosote metabolism, Culture Media, Hydrocarbons, Aromatic analysis, Molecular Structure, Polycyclic Aromatic Hydrocarbons analysis, Soil Microbiology, Phenanthrenes metabolism, Sphingomonas classification, Sphingomonas growth & development, Sphingomonas isolation & purification, Sphingomonas metabolism
- Abstract
A bacterial strain capable of utilizing 3,6-dimethylphenanthrene (3,6-DMP) as its sole source of carbon and energy was isolated from a creosote-contaminated soil. The isolate was identified as a strain of Sphingomonas sp. and was designated strain JS1. Utilization of 3,6-DMP was demonstrated by an increase in bacterial biomass concomitant with a decrease in 3,6-DMP in a liquid mineral medium with this compound as its sole source of carbon and energy. Strain JS1 showed a high specificity in the use of the most abundant alkylderivatives of crude oils, such as alkylnaphthalenes and other alkylphenanthrenes, as the sole source of carbon and energy. It can also use several polycyclic aromatic hydrocarbons of three and four rings and their alkylated derivatives as growth substrates or transform them. The identification of several intermediate metabolites points to extensive metabolic activity, including the following: (i) aromatic ring oxidation and cleavage, (ii) methyl group oxidations, and (iii) methylenic oxidations. The metabolic actions of Sphingomonas sp. JS1 on the aromatic fraction extracted from a creosote-contaminated soil are also examined.
- Published
- 2003
- Full Text
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18. Biodegradation of a crude oil by three microbial consortia of different origins and metabolic capabilities.
- Author
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Viñas M, Grifoll M, Sabaté J, and Solanas AM
- Subjects
- Biodegradation, Environmental, Gas Chromatography-Mass Spectrometry, Hydrocarbons metabolism, Kinetics, Soil Microbiology, Substrate Specificity, Time Factors, Bacteria metabolism, Environmental Pollution prevention & control, Petroleum metabolism
- Abstract
Microbial consortia were obtained three by sequential enrichment using different oil products. Consortium F1AA was obtained on a heavily saturated fraction of a degraded crude oil; consortium TD, by enrichment on diesel and consortium AM, on a mixture of five polycyclic aromatic hydrocarbons [PAHs]. The three consortia were incubated with a crude oil in order to elucidate their metabolic capabilities and to investigate possible differences in the biodegradation of these complex hydrocarbon mixtures in relation to their origin. The efficiency of the three consortia in removing the saturated fraction was 60% (F1AA), 48% (TD) and 34% (AM), depending on the carbon sources used in the enrichment procedures. Consortia F1AA and TD removed 100% of n-alkanes and branched alkanes, whereas with consortium AM, 91% of branched alkanes remained. Efficiency on the polyaromatic fraction was 19% (AM), 11% (TD) and 7% (F1AA). The increase in aromaticity of the polyaromatic fraction during degradation of the crude oil by consortium F1AA suggested that this consortium metabolized the aromatic compounds primarily by oxidation of the alkylic chains. The 500-fold amplification of the inocula from the consortia by subculturing in rich media, necessary for use of the consortia in bioremediation experiments, showed no significant decrease in their degradation capability.
- Published
- 2002
- Full Text
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19. Identification of a novel metabolite in the degradation of pyrene by Mycobacterium sp. strain AP1: actions of the isolate on two- and three-ring polycyclic aromatic hydrocarbons.
- Author
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Vila J, López Z, Sabaté J, Minguillón C, Solanas AM, and Grifoll M
- Subjects
- Biodegradation, Environmental, Environmental Pollution, Mycobacterium classification, Mycobacterium growth & development, Mycobacterium isolation & purification, Petroleum, Polycyclic Aromatic Hydrocarbons chemistry, Pyrenes chemistry, Silicon Dioxide analysis, Mycobacterium metabolism, Polycyclic Aromatic Hydrocarbons metabolism, Pyrenes metabolism
- Abstract
Mycobacterium sp. strain AP1 grew with pyrene as a sole source of carbon and energy. The identification of metabolites accumulating during growth suggests that this strain initiates its attack on pyrene by either monooxygenation or dioxygenation at its C-4, C-5 positions to give trans- or cis-4,5-dihydroxy-4,5-dihydropyrene, respectively. Dehydrogenation of the latter, ortho cleavage of the resulting diol to form phenanthrene 4,5-dicarboxylic acid, and subsequent decarboxylation to phenanthrene 4-carboxylic acid lead to degradation of the phenanthrene 4-carboxylic acid via phthalate. A novel metabolite identified as 6,6'-dihydroxy-2,2'-biphenyl dicarboxylic acid demonstrates a new branch in the pathway that involves the cleavage of both central rings of pyrene. In addition to pyrene, strain AP1 utilized hexadecane, phenanthrene, and fluoranthene for growth. Pyrene-grown cells oxidized the methylenic groups of fluorene and acenaphthene and catalyzed the dihydroxylation and ortho cleavage of one of the rings of naphthalene and phenanthrene to give 2-carboxycinnamic and diphenic acids, respectively. The catabolic versatility of strain AP1 and its use of ortho cleavage mechanisms during the degradation of polycyclic aromatic hydrocarbons (PAHs) give new insight into the role that pyrene-degrading bacterial strains may play in the environmental fate of PAH mixtures.
- Published
- 2001
- Full Text
- View/download PDF
20. Photolysis of PAHs in aqueous phase by UV irradiation.
- Author
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Sabaté J, Bayona JM, and Solanas AM
- Subjects
- Kinetics, Oxidants, Photochemical chemistry, Oxidation-Reduction, Photolysis, Titanium chemistry, Ultraviolet Rays, Environmental Pollutants analysis, Polycyclic Aromatic Hydrocarbons chemistry
- Abstract
The photooxidation of polycyclic aromatic hydrocarbons (PAHs) was investigated in an aqueous ethanolic solution irradiated with a medium-pressure mercury lamp in laboratory photoreactors equipped with a quartz immersion well. Degradation photolysis of fluorene was more efficient than sensitized photolytic oxidation in the presence of TiO2 suspensions. Photolysis kinetics was dependent on molecular weight and the presence and type of substituents. During the photolytic degradation of fluorene and its derivatives, 9-fluorenone and its corresponding derivatives, which were more resistant to photolysis, were formed.
- Published
- 2001
- Full Text
- View/download PDF
21. Evaluation of acute toxicity and genotoxicity of liquid products from pyrolysis of Eucalyptus grandis wood.
- Author
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Pimenta AS, Bayona JM, García MT, and Solanas AM
- Subjects
- Animals, Biological Assay, Daphnia drug effects, Enzyme Activation, Gas Chromatography-Mass Spectrometry, Hot Temperature, Liver drug effects, Liver enzymology, Molecular Structure, Mutagenicity Tests, Phenols chemistry, Phenols toxicity, Polycyclic Aromatic Hydrocarbons toxicity, Rats, Vibrio drug effects, Wood, Eucalyptus chemistry, Plants, Medicinal
- Abstract
Slow pyrolysis of Eucalyptus grandis wood was performed in an oven laboratory, and smoke was trapped and condensed to yield liquid products. Polycyclic aromatic hydrocarbons (PAHs) and phenolic fractions were isolated from the former liquid products using adsorption column chromatography (ACC) and identified by GC/MS. Concentrations of PAH and phenolic fractions in total pyrolysis liquids were respectively 48.9 microg/g and 8.59% (w/w). Acute toxicity of total samples of pyrolysis liquids and the phenolic fraction was evaluated by means of two bioassays, namely, 24-h immobilization bioassay with Daphnia magna and Microtox bioassays, the latter employing the luminescent bacteria Photobacterium phosphoreum. Total pyrolysis liquids and the PAH fraction were evaluated for genotoxicity by the Microtox bioassay conducted using rehydrated freeze-dried dark mutant of the luminescent bacteria Vibrio fisheri strain M169. Total pyrolysis liquids and the phenolic fraction, respectively, in concentrations of 170 and 68 mg/L were able to immobilize 50% (EC(50)) of the D. magna population following 24-h exposure. Concentrations of 19 and 6 mg/L, respectively, for total pyrolysis liquids and phenolic fraction were the effective concentrations that resulted in a 50% (EC(50)) reduction in light produced by bacteria in the Microtox bioassay. Accordingly, the Microtox bioassay was more sensitive to toxic effects of both kind of samples than the D. magna bioassay, particularly for the phenolic fraction. Regarding to the genotoxicity evaluation, the results achieved by Microtox bioassay showed that total pyrolysis liquids had no genotoxic effects with and without exogenous metabolic activation using rat liver homogenate (S9). However, the PAH fraction showed toxic effects with rat liver activation and had a dose-response number (DRN) equal to 1.6, being in this way suspected genotoxic. The lowest detected concentration (LDC) of the PAH fraction able to cause genotoxic effects was 375 microg/L.
- Published
- 2000
- Full Text
- View/download PDF
22. New metabolites in the degradation of fluorene by Arthrobacter sp. strain F101.
- Author
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Casellas M, Grifoll M, Bayona JM, and Solanas AM
- Subjects
- Chromatography, High Pressure Liquid, Gas Chromatography-Mass Spectrometry, Arthrobacter metabolism, Fluorenes metabolism
- Abstract
Identification of new metabolites and demonstration of key enzyme activities support and extend the pathways previously reported for fluorene metabolism by Arthrobacter sp. strain F101. Washed-cell suspensions of strain F101 with fluorene accumulated 9-fluorenone, 4-hydroxy-9-fluorenone, 3-hydroxy-1-indanone, 1-indanone, 2-indanone, 3-(2-hydroxyphenyl) propionate, and a compound tentatively identified as a formyl indanone. Incubations with 2-indanone produced 3-isochromanone. The growth yield with fluorene as a sole source of carbon and energy corresponded to an assimilation of about 34% of fluorene carbon. About 7.4% was transformed into 9-fluorenol, 9-fluorenone, and 4-hydroxy-9-fluorenone. Crude extracts from fluorene-induced cells showed 3,4-dihydrocoumarin hydrolase and catechol 2,3-dioxygenase activities. These results and biodegradation experiments with the identified metabolites indicate that metabolism of fluorene by Arthrobacter sp. strain F101 proceeds through three independent pathways. Two productive routes are initiated by dioxygenation at positions 1,2 and 3,4, respectively. meta cleavage followed by an aldolase reaction and loss of C-1 yield the detected indanones. Subsequent biological Baeyer-Villiger reactions produce the aromatic lactones 3,4-dihydrocoumarin and 3-isochromanone. Enzymatic hydrolysis of the former gives 3-(2-hydroxyphenyl) propionate, which could be a substrate for a beta oxidation cycle, to give salicylate. Further oxidation of the latter via catechol and 2-hydroxymuconic semialdehyde connects with the central metabolism, allowing the utilization of all fluorene carbons. Identification of 4-hydroxy-9-fluorenone is consistent with an alternative pathway initiated by monooxygenation at C-9 to give 9-fluorenol and then 9-fluorenone. Although dioxygenation at 3,4 positions of the ketone apparently occurs, this reaction fails to furnish a subsequent productive oxidation of this compound.
- Published
- 1997
- Full Text
- View/download PDF
23. Bioassay-directed chemical analysis of genotoxic components in urban airborne particulate matter from Barcelona (Spain).
- Author
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Casellas M, Fernandez P, Bayona JM, and Solanas AM
- Subjects
- Chromatography, Liquid methods, Environmental Monitoring methods, Gas Chromatography-Mass Spectrometry methods, Mutagenicity Tests methods, Salmonella typhimurium genetics, Spain, Air Pollutants analysis, Mutagens analysis
- Abstract
Organic extracts of airborne particulate matter, collected in the city of Barcelona, were subjected to three-level, bioassay-directed, chemical fractionation, including gel permeation chromatography (GPC) and normal-phase (NP) and reversed-phase (RP) liquid chromatography (LC). The chemical characterization, directed by the Salmonella microsome mutagenicity assay (TA98, TA98NR and TA98/1,8DNP6 +/- S9), was carried out by capillary GC (CGC) coupled to selective detection systems, and by GC-MS techniques. The results obtained with the nitroreductase deficient strains show the important contribution of nitroaromatic compounds. Detailed chemical analysis of the mutagenic fractions led to the identification of 82 aromatic compounds and revealed the large contribution of chemical classes that are more polar than polycyclic aromatic hydrocarbons such as aromatic ketones, quinones and aldehydes.
- Published
- 1995
- Full Text
- View/download PDF
24. Sources and seasonal variability of mutagenic agents in the Barcelona City aerosol.
- Author
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Bayona JM, Casellas M, Fernández P, Solanas AM, and Albaigés J
- Subjects
- Air Pollutants toxicity, Mutagenicity Tests, Polycyclic Compounds toxicity, Salmonella typhimurium drug effects, Spain, Vehicle Emissions, Air Pollutants analysis, Polycyclic Compounds analysis, Seasons
- Abstract
Organic extracts (dichloromethane) isolated from airborne particulate matter, collected in two sampling sites located in the Barcelona City, were mutagenic in the Salmonella typhimurium (TA98 +/-S9) bioassay. The highest direct-acting mutagenicity (69-78 rev m-3) was detected during fall and spring, which corresponds to the highest levels of mutagenic nitroarenes (248 to 350 pg m-3). On the other hand, the highest level of indirect-acting mutagenicity was obtained in summer, paralleling with the highest concentrations of polycyclic aromatic ketones and polycyclic aromatic quinones. Furthermore, the sources of PAH in the urban particulate matter were estimated from the ratio of the less reactive components (i.e. benzofluranthenes/benzo[e]pyrene, indeno[1,2,3-cd]pyrene/benzo[ghi]perylene, methylphenantherenes/phenanthrene) and reflected a predominance of pyrolytic mobile sources (i.e. vehicular emissions). Nevertheless, a contribution of stationary sources in winter was also apparent. Finally, the seasonal variability of polycyclic aromatic ketones, quinones, aromatic lactones and aldehydes reflected a major contribution of the atmospheric transformation processes from related PAH rather than a direct emission from combustion sources.
- Published
- 1994
- Full Text
- View/download PDF
25. Isolation and characterization of a fluorene-degrading bacterium: identification of ring oxidation and ring fission products.
- Author
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Grifoll M, Casellas M, Bayona JM, and Solanas AM
- Subjects
- Arthrobacter isolation & purification, Arthrobacter ultrastructure, Biodegradation, Environmental, Oxidation-Reduction, Water Microbiology, Arthrobacter metabolism, Fluorenes metabolism
- Abstract
An Arthrobacter sp. strain, F101, able to use fluorene as the sole source of carbon and energy, was isolated from sludge from an oil refinery wastewater treatment plant. During growth in the presence of fluorene, four major metabolites were detected and isolated by thin-layer chromatography and high-performance liquid chromatography. 9-Fluorenol, 9H-fluoren-9-one, and 3,4-dihydrocoumarin were identified by UV spectra, mass spectrometry, and 300-MHz proton nuclear magnetic resonance. The fourth metabolite has been characterized, but precise identification was not possible. Since strain F101 is not able to grow with fluorenone, two different pathways of fluorene biodegradation are suggested: one supports cell growth and produces 3,4-dihydrocoumarin as an intermediate and probably the unidentified metabolite, and the other produces 9-fluorenol and 9H-fluoren-9-one and appears to be a dead-end route.
- Published
- 1992
- Full Text
- View/download PDF
26. Bioassay-directed chemical characterization of genotoxic agents in the dissolved and particulate water phases of the Besos and Llobregat Rivers (Barcelona, Spain).
- Author
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Grifoll M, Solanas AM, and Bayona JM
- Subjects
- Biological Assay, Solubility, Spain, Fresh Water chemistry, Mutagens chemistry, Water Pollutants, Chemical toxicity
- Abstract
Particulate (greater than 0.22 microns) and dissolved phases of water concentrates (600 mL) of Llobregat and Besos Rivers (Barcelona, Spain), were tested in the Salmonella/microsome assay, tester strains TA98 and TA100. Most of them showed significant mutagenic activity. However, independently of the application of exogenous metabolic activation, the dimethylsulfoxide extracts of the particulate matter exhibited a stronger mutagenic activity than the dissolved phase. This indicated that both rivers are chronically polluted by frameshift and base-pair substitution mutagens and promutagens. In order to investigate their identity, a bioassay-directed column chromatography fractionation of the base-neutrals isolated from the dissolved and particulate phases of Besos river water (7 L) was carried out. The mutagenic activity (TA98) was higher in presence of S9 and was recovered in the more polar fractions, where several mutagenic agents were identified by capillary GC-MS in the negative ion chemical ionization mode (NICI). Among them, o-tolidine, nitroquinoline, nitroaniline, dichlorobenzidine and several aromatic quinones were candidates for fraction mutagenicity.
- Published
- 1992
- Full Text
- View/download PDF
27. Characterization of genotoxic components in sediments by mass spectrometric techniques combined with Salmonella/microsome test.
- Author
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Grifoll M, Solanas AM, and Bayona JM
- Subjects
- Gas Chromatography-Mass Spectrometry, Mutagenicity Tests, Salmonella drug effects, Spain, Soil Pollutants toxicity, Water Pollutants toxicity, Water Pollutants, Chemical toxicity
- Abstract
The application of Salmonella/microsomal mammalian tests to column chromatography fractions isolated from river and marine sediments collected in the vicinity of Barcelona city, Spain, demonstrated a positive response (TA98 + S9 mix) among the polar fractions. Chemical analysis by high resolution gas chromatography coupled to negative ion chemical ionization mass spectrometry (HRGC-NICI MS) provided sensitivity and selectivity to detect several mutagenic chemical classes. Among them, nitrated PAHs, azaarenes, aromatic amines, anhydrides, and ketones were identified. A total of 116 compounds were tentatively identified, 22 for the first time, of which 16 possessed mutagenic activity. However, a lack of correlation between chemical composition and fraction mutagenicity in the medium polarity fractions, especially in the river sediment, was evidenced. The occurrence of multiple interactions between components in spiked organic extracts is demonstrated.
- Published
- 1990
- Full Text
- View/download PDF
28. A comparative study of chemical and microbiological monitoring of pollutant hydrocarbons in urban aquatic environments.
- Author
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Solanas AM, Parés R, Marfil C, and Albaigés J
- Subjects
- Seasons, Seawater analysis, Spain, Hydrocarbons analysis, Water Microbiology, Water Pollutants analysis, Water Pollutants, Chemical analysis
- Abstract
Conventional chemical and microbiological methods-aromatics by UV-fluorescence and the number of oil-degrading microorganisms, respectively-have been used for the monitoring of pollutant hydrocarbons in three different aquatic systems: two rivers, one harbour and three marine coastal areas. An evaluation of the first year data of such study is presented. Relative populations of total heterotrophic microorganisms and those of degrading hydrocarbons correlate satisfactorily with hydrocarbon concentrations in marine areas, where chronic pollution situations occur, whereas unreliable results were obtained in the river systems. The water temperature seems to have a positive influence on the response of microorganisms to oil pollution.
- Published
- 1982
- Full Text
- View/download PDF
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