1. Isolation of two cytokinin metabolites from the rhizosphere of Norway spruce seedlings (Picea abies L. Karst.)
- Author
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Klaus von Schwartzenberg, M. Bonnet-Masimbert, Patrick Doumas, Laboratoire de Biologie Cellulaire, Assistance Publique - Hôpitaux de Marseille (APHM)- Hôpital de la Timone [CHU - APHM] (TIMONE), Unité de recherche Amélioration, Génétique et Physiologie Forestières (AGPF), Institut National de la Recherche Agronomique (INRA), ProdInra, Archive Ouverte, Hôpital de la Timone [CHU - APHM] (TIMONE)-Assistance Publique - Hôpitaux de Marseille (APHM), and Unité de recherche Amélioration, Génétique et Physiologie Forestières (UAGPF)
- Subjects
0106 biological sciences ,arbre forestier ,Physiology ,gas chromatography ,Metabolite ,mass spectrum analysis ,chromatographie en phase gazeuse ,Hydroculture ,Plant Science ,résineux ,forest tree ,01 natural sciences ,cytokinin ,03 medical and health sciences ,chemistry.chemical_compound ,spectrométrie de masse ,Botany ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,[SDV.BV] Life Sciences [q-bio]/Vegetal Biology ,métabolisme ,030304 developmental biology ,EPICEA COMMUN ,0303 health sciences ,Rhizosphere ,cytokinine ,biology ,softwood forest tree ,Plant physiology ,Picea abies ,biology.organism_classification ,chemistry ,Biochemistry ,Cytokinin ,Isopentenyladenosine ,Zeatin ,picea abies ,rhizosphère ,Agronomy and Crop Science ,010606 plant biology & botany - Abstract
Roots of young Norway spruce seedlings were incubated under hydroculture conditions in a synthetic nutrient medium containing either 3H-isopentenyladenosine, isopentenyladenosine or zeatin riboside. When feeding with 3H-isopentenyladenosine a new radiaolabelled metabolite was found in the feeding solution as well as in root extracts. Isopentenyladenosine and zeatin riboside were metabolised and for both compounds an unknown metabolite was detected in the feeding solution. The metabolites were purified by solid phase extraction, HPLC and partially characterised. A major characteristic of the metabolites is their reactivity in the presence of NH4OH, which results in the formation of the cytokinin bases isopentenyladenine or zeatin, respectively. UV-spectra and the chemical characteristics indicate that the new metabolites are closely related. The GC-MS analysis revealed, that the metabolites are true derivatives of isopentenyladenine and zeatin. The biogenesis of the new metabolites is discussed with regard to plant microbial interactions.
- Published
- 1994
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