1. Integrating sodium cholate-modified MOF hybrid lipase and solubilization of hydrophobic candidates into a step for liganding fishing lipase inhibitors from Nelumbinis Folium.
- Author
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Hu W, Li J, Zhang X, Lv Y, Ye H, Li C, Liu E, and Chu C
- Subjects
- Ligands, Alkaloids chemistry, Alkaloids pharmacology, Plant Extracts chemistry, Plant Extracts pharmacology, Lipase antagonists & inhibitors, Lipase chemistry, Lipase metabolism, Molecular Docking Simulation, Enzymes, Immobilized chemistry, Enzymes, Immobilized antagonists & inhibitors, Metal-Organic Frameworks chemistry, Sodium Cholate chemistry, Hydrophobic and Hydrophilic Interactions, Flavonoids chemistry, Flavonoids pharmacology, Solubility, Enzyme Inhibitors pharmacology, Enzyme Inhibitors chemistry
- Abstract
Enzyme immobilization by metal organic frameworks (MOFs) is an efficient way for screening active constituents in natural products. However, the enzyme's biocatalysis activity is usually decreased due to unfavorable conformational changes during the immobilization process. In this study, sodium cholate was firstly used as the modifier for zeolitic imidazolate framework-8 (ZIF-8) immobilized lipase to increase both the stability and activity. More importantly, with the help of solubilization of sodium cholate, a total of 3 flavonoids and 6 alkaloids candidate compounds were fished out. Their structures were identified and the enzyme inhibitory activities were verified. In addition, the binding information between the candidate compound and the enzyme was displayed by molecular docking. This study provides valuable information for the improvement of immobilized enzyme activity and functional active ingredients in complicated medicinal plant extracts., Competing Interests: Declaration of Competing Interest The authors declare that they have no conflicts of interest., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
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