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2. Embryo Quality Conditions the Secretory Profile of Tolerogenic Dendritic Cell DC‐10 During the Peri‐Implantation Period.

Author

Gori, Soledad, Fernández, Laura, Soczewski, Elizabeth, Schafir, Ana, Castagnola, Lara, Grasso, Esteban, Martínez, Gustavo, Leirós, Claudia Pérez, and Ramhorst, Rosanna

Subjects
EMBRYO implantation, MYELOID cells, DENDRITIC cells, STROMAL cells, MEDROXYPROGESTERONE
Abstract

Problem: The decidualization process conditions monocytes to the immunosuppressive and tolerogenic dendritic cell (DC)‐10 profile, a DC subset with high IL‐10 production. Since the implantation process implies an embryo‐endometrium‐immune crosstalk, here we focused on the ability of embryonic soluble factors to modify decidual DC conditioning accordingly with its quality. Method of Study: Human endometrial stromal cell line (HESC) decidualized with medroxyprogesterone and dibutyryl‐cAMP (Dec) was stimulated with human embryo‐conditioned media (ECM), classified as normal (ND) or impaired developed (ID) for 48 h (n = 18/group). Monocytes isolated from six healthy women were differentiated to DCs with rhGM‐CSF+rhIL‐4 in the presence/absence of conditioned media (CM) from decidualized cells stimulated with ECM or nontreated. Results: We found that decidualized cells stimulated with ECM sustain a myeloid regulatory cell profile on monocyte‐derived culture with increased frequency of CD1a‐CD14+ and CD83+CD86low cells. ND‐Dec sustained the higher expression of the DC‐10 markers, HLA‐G and IL‐10 whereas ID‐Dec diminished IL‐10 production (ID‐Dec: 135 ± 37.4 vs. Dec: 223.3 ± 49.9 pg/mL, p < 0.05). The treatment with ECM‐Dec sustained a higher IL‐10 production and prevented the increase of CD83/CD86 after LPS challenge regardless of embryo quality. Notably, TNF‐α production increased in ID‐Dec cultures (ID‐Dec: 475.1 ± 134.7 vs. Dec: 347.5 ± 98 pg/mL, p < 0.05). Conclusions: Although remaining in a tolerogenic profile compatible with DC‐10, DCs can differentially respond to decidual secreted factors based on embryo quality, changing their secretome. These results suggest that in the presence of arrested embryo, DCs could differentially shape the immunological microenvironment, contributing to arrested embryo clearance during the menstrual phase. [ABSTRACT FROM AUTHOR]

Published
2024
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5. miRNAs associated to endoplasmic reticulum stress and unfolded protein response during decidualization

Author

Soczewski, Elizabeth, primary, Murrieta-Coxca, José Martin, additional, Miranda, Lucas, additional, Fuentes-Zacarías, Paulina, additional, Gutiérrez-Samudio, Ruby, additional, Grasso, Esteban, additional, Marti, Marcelo, additional, Leirós, Claudia Pérez, additional, Morales-Prieto, Diana, additional, Markert, Udo R., additional, and Ramhorst, Rosanna, additional

Published
2023
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6. Endoplasmic reticulum stress induces an inflammatory response: Potential regulation by miRNAs

Author

Soczewski, Elizabeth, primary, Murrieta-Coxca, José Martin, additional, Zacarías-Fuentes, Paulina, additional, Gutiérrez-Samudio, Ruby, additional, Miranda, Lucas, additional, Grasso, Esteban, additional, Martí, Marcelo, additional, Morales-Prieto, Diana, additional, Leirós, Claudia Pérez, additional, Market, Udo, additional, and Ramhorst, Rosanna, additional

Published
2022
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7. Understanding the natural selection of human embryos: Blastocyst quality modulates the inflammatory response during the peri‐implantation period

Author

Fernández, Laura, primary, Grasso, Esteban, additional, Soczewski, Elizabeth, additional, Gori, Soledad, additional, Calo, Guillermina, additional, Hauk, Vanesa, additional, Sabbione, Florencia, additional, Gallino, Lucila, additional, Martínez, Gustavo, additional, Irigoyen, Marcela, additional, Bestach, Yesica, additional, Pérez Leirós, Claudia, additional, and Ramhorst, Rosanna, additional

Published
2021
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8. Placental extracellular vesicles: their potential as biomarkers of gestational complications

Author

Lara, Brenda Ayelen, Merech, Fátima Isabel, Vota, Daiana Marina, Calo, Guillermina, Soczewski, Elizabeth Victoria, Grasso, Esteban Nicolas, Perez Leiros, Claudia, and Hauk, Vanesa Cintia

Subjects
placenta, patologias gestacionales, purl.org/becyt/ford/3 [https], purl.org/becyt/ford/3.1 [https], vesiculas extracelulares
Abstract

Las vesículas extracelulares son importantes mediadores de la comunicación intercelular. Durante la gestación,diversos tipos celulares placentarios como no placentariossecretan vesículas extracelulares a la circulación. Se ha demostrado que las células trofoblásticas liberan permanentemente a la circulación materna vesículas extracelularescargadas con diversas moléculas que modulan la función delas células trofoblásticas, inmunes y vasculares, entre otras.Las complicaciones gestacionales que afectan la placentación, como la restricción del crecimiento intrauterino(RCIU) y la preeclampsia (PE), son un problema de saludpública mundial, ya que son condiciones que contribuyen,en alto grado, a la morbimortalidad materna y neonatal.Hasta ahora, no se dispone de pruebas clínicas quepermitan anticipar el diagnóstico y/o proveer indicadorespredictivos para identificar a las madres con mayor riesgode desarrollar estas complicaciones. En los últimos años,se ha demostrado el potencial de las vesículas extracelulares y su contenido como potenciales biomarcadoresde distintas patologías, por lo que su aplicación despiertaespecial interés en las complicaciones del embarazo. Extracellular vesicles are significant mediators of cellto-cell communication. During pregnancy, placental and non-placental cell types release extracellular vesicles into circulation. Is has been demonstrated that trophoblast cells are continuously releasing vesicles loaded with factors modulating trophoblast, immune and vascular functions. Gestational complications associated to impaired placentation, such as intrauterine growth restriction (IUGR) and preeclampsia (PE), are a global public health problem since they are major contributors to maternal and neonatal morbidity and mortality. Currently, there are no clinical tests that allow early diagnosis and/or provide predictive markers to identify mothers at higher risk of developing these complications. The potential of extracellular vesicles and their content as biomarkers for different pathologies has been demonstrated. For this reason nowadays their application in gestational complications is of special interest. Fil: Lara, Brenda Ayelen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina Fil: Merech, Fátima Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina Fil: Vota, Daiana Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina Fil: Calo, Guillermina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina Fil: Soczewski, Elizabeth Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina Fil: Grasso, Esteban Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina Fil: Perez Leiros, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina Fil: Hauk, Vanesa Cintia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina

Published
2020

11. Understanding the natural selection of human embryos: Blastocyst quality modulates the inflammatory response during the peri‐implantation period.

Author

Fernández, Laura, Grasso, Esteban, Soczewski, Elizabeth, Gori, Soledad, Calo, Guillermina, Hauk, Vanesa, Sabbione, Florencia, Gallino, Lucila, Martínez, Gustavo, Irigoyen, Marcela, Bestach, Yesica, Pérez Leirós, Claudia, and Ramhorst, Rosanna

Subjects
HUMAN embryos, NATURAL selection, REGULATORY T cells, INFLAMMATION, BLASTOCYST
Abstract

Problem: Decidualized cells display an active role during embryo implantation sensing blastocyst quality, allowing the implantation of normal developed blastocysts and preventing the invasion of impaired developed ones. Here, we characterized the immune microenvironment generated by decidualized cells in response to soluble factors secreted by blastocysts that shape the receptive milieu. Method of Study: We used an in vitro model of decidualization based on the Human Endometrial Stromal Cells line (HESC) differentiated with medroxiprogesterone and dibutyryl‐cAMP, then treated with human blastocysts‐conditioned media (BCM) classified according to their quality. Results: Decidualized cells treated with BCM from impaired developed blastocysts increased IL‐1β production. Next, we evaluated the ability of decidualized cells to modulate other mediators associated with menstruation as chemokines. Decidualized cells responded to stimulation with BCM from impaired developed blastocysts increasing CXCL12 expression and CXCL8 secretion. The modulation of these markers was associated with the recruitment and activation of neutrophils, while regulatory T cells recruitment was restrained. These changes were not observed in the presence of BCM from normal developed blastocysts. Conclusion: Soluble factors released by impaired developed blastocysts induce an exacerbated inflammatory response associated with neutrophils recruitment and activation, providing new clues to understand the molecular basis of the embryo‐endometrial dialogue. [ABSTRACT FROM AUTHOR]

Published
2022
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12. VIP Promotes Recruitment of Tregs to the Uterine–Placental Interface During the Peri-Implantation Period to Sustain a Tolerogenic Microenvironment

Author

Gallino, Lucila, primary, Hauk, Vanesa, additional, Fernández, Laura, additional, Soczewski, Elizabeth, additional, Gori, Soledad, additional, Grasso, Esteban, additional, Calo, Guillermina, additional, Saraco, Nora, additional, Berensztein, Esperanza, additional, Waschek, James A., additional, Pérez Leirós, Claudia, additional, and Ramhorst, Rosanna, additional

Published
2020
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15. The decidualization by VIP conditions endometrial receptivity and immune microenvironment through Reticular Stress and Unfolded Protein Response

Author

Gori, Soledad, primary, Soczewski, Elizabeth, additional, Grasso, Esteban, additional, Fernandez, Laura, additional, Gallino, Lucila, additional, Irigoyen, Marcela, additional, Lobo, Thalita F., additional, Salamone, Gabriela, additional, Mattar, Rosiane, additional, Daher, Silvia, additional, Leiros, Claudia Perez, additional, and Ramhorst, Rosanna, additional

Published
2019
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