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17 results on '"Snoo, C. Bastiaan"'

3. The genomic landscape of meiotic crossovers and gene conversions in Arabidopsis thaliana

Author

Wijnker, Erik, James, Geo Velikkakam, Ding, Jia, Becker, Frank, Klasen, Jonas R., Rawat, Vimal, Rowan, Beth A., de Jong, Daniel F., de Snoo, C. Bastiaan, Zapata, Luis, Huettel, Bruno, de Jong, Hans, Ossowski, Stephan, Weigel, Detlef, Koornneef, Maarten, Keurentjes, Joost J. B., and Schneeberger, Korbinian

Subjects
Quantitative Biology - Genomics
Abstract

Knowledge of the exact distribution of meiotic crossovers (COs) and gene conversions (GCs) is essential for understanding many aspects of population genetics and evolution, from haplotype structure and long-distance genetic linkage to the generation of new allelic variants of genes. To this end, we resequenced the four products of 13 meiotic tetrads along with 10 doubled haploids derived from Arabidopsis thaliana hybrids. GC detection through short reads has previously been confounded by genomic rearrangements. Rigid filtering for misaligned reads allowed GC identification at high accuracy and revealed an ~80-kb transposition, which undergoes copy-number changes mediated by meiotic recombination. Non-crossover associated GCs were extremely rare most likely due to their short average length of ~25-50 bp, which is significantly shorter than the length of CO associated GCs. Overall, recombination preferentially targeted non-methylated nucleosome-free regions at gene promoters, which showed significant enrichment of two sequence motifs., Comment: 44 pages, 5 figures with figure supplements

Published
2013

6. Meiotic crossover reduction by virus‐induced gene silencing enables the efficient generation of chromosome substitution lines and reverse breeding inArabidopsis thaliana

Author

Calvo‐Baltanás, Vanesa, primary, Wijnen, Cris L., additional, Yang, Chao, additional, Lukhovitskaya, Nina, additional, Snoo, C. Bastiaan, additional, Hohenwarter, Linus, additional, Keurentjes, Joost J. B., additional, Jong, Hans, additional, Schnittger, Arp, additional, and Wijnker, Erik, additional

Published
2020
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7. Meiotic crossover reduction by virus‐induced gene silencing enables the efficient generation of chromosome substitution lines and reverse breeding in Arabidopsis thaliana.

Author

Calvo‐Baltanás, Vanesa, Wijnen, Cris L., Yang, Chao, Lukhovitskaya, Nina, Snoo, C. Bastiaan, Hohenwarter, Linus, Keurentjes, Joost J. B., Jong, Hans, Schnittger, Arp, and Wijnker, Erik

Subjects
GENE silencing, ARABIDOPSIS thaliana, PLANT hybridization, HOMOLOGOUS chromosomes, PLANT breeding, HAPLOIDY
Abstract

Summary: Plant breeding applications exploiting meiotic mutant phenotypes (like the increase or decrease of crossover (CO) recombination) have been proposed over the last years. As recessive meiotic mutations in breeding lines may affect fertility or have other pleiotropic effects, transient silencing techniques may be preferred. Reverse breeding is a breeding technique that would benefit from the transient downregulation of CO formation. The technique is essentially the opposite of plant hybridization: a method to extract parental lines from a hybrid. The method can also be used to efficiently generate chromosome substitution lines (CSLs). For successful reverse breeding, the two homologous chromosome sets of a heterozygous plant must be divided over two haploid complements, which can be achieved by the suppression of meiotic CO recombination and the subsequent production of doubled haploid plants. Here we show the feasibility of transiently reducing CO formation using virus‐induced gene silencing (VIGS) by targeting the meiotic gene MSH5 in a wild‐type heterozygote of Arabidopsis thaliana. The application of VIGS (rather than using lengthy stable transformation) generates transgene‐free offspring with the desired genetic composition: we obtained parental lines from a wild‐type heterozygous F1 in two generations. In addition, we obtained 20 (of the 32 possible) CSLs in one experiment. Our results demonstrate that meiosis can be modulated at will in A. thaliana to generate CSLs and parental lines rapidly for hybrid breeding. Furthermore, we illustrate how the modification of meiosis using VIGS can open routes to develop efficient plant breeding strategies. [ABSTRACT FROM AUTHOR]

Published
2020
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11. Hybrid recreation by reverse breeding in Arabidopsis thaliana

Author

Wijnker, Erik, primary, Deurhof, Laurens, additional, van de Belt, Jose, additional, de Snoo, C Bastiaan, additional, Blankestijn, Hetty, additional, Becker, Frank, additional, Ravi, Maruthachalam, additional, Chan, Simon W L, additional, van Dun, Kees, additional, Lelivelt, Cilia L C, additional, de Jong, Hans, additional, Dirks, Rob, additional, and Keurentjes, Joost J B, additional

Published
2014
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12. The genomic landscape of meiotic crossovers and gene conversions in Arabidopsis thaliana

Author

Wijnker, Erik, primary, Velikkakam James, Geo, additional, Ding, Jia, additional, Becker, Frank, additional, Klasen, Jonas R, additional, Rawat, Vimal, additional, Rowan, Beth A, additional, de Jong, Daniël F, additional, de Snoo, C Bastiaan, additional, Zapata, Luis, additional, Huettel, Bruno, additional, de Jong, Hans, additional, Ossowski, Stephan, additional, Weigel, Detlef, additional, Koornneef, Maarten, additional, Keurentjes, Joost JB, additional, and Schneeberger, Korbinian, additional

Published
2013
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13. Author response: The genomic landscape of meiotic crossovers and gene conversions in Arabidopsis thaliana

Author

Wijnker, Erik, primary, Velikkakam James, Geo, additional, Ding, Jia, additional, Becker, Frank, additional, Klasen, Jonas R, additional, Rawat, Vimal, additional, Rowan, Beth A, additional, de Jong, Daniël F, additional, de Snoo, C Bastiaan, additional, Zapata, Luis, additional, Huettel, Bruno, additional, de Jong, Hans, additional, Ossowski, Stephan, additional, Weigel, Detlef, additional, Koornneef, Maarten, additional, Keurentjes, Joost JB, additional, and Schneeberger, Korbinian, additional

Published
2013
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14. Stable plastid transformation in lettuce (Lactuca sativa L.)

Author

Lelivelt, Cilia L.C., McCabe, Matthew S., Newell, Christine A., de Snoo, C. Bastiaan, van Dun, Kees M.P., Birch-Machin, Ian, Gray, John C., Mills, Kingston H.G., Nugent, Jacqueline M., Lelivelt, Cilia L.C., McCabe, Matthew S., Newell, Christine A., de Snoo, C. Bastiaan, van Dun, Kees M.P., Birch-Machin, Ian, Gray, John C., Mills, Kingston H.G., and Nugent, Jacqueline M.

Abstract

Although plastid transformation in higher plants was first demonstrated in the early 1990s it is only recently that the technology is being extended to a broader range of species. To date, the production of fertile transplastomic plants has been reported for tobacco, tomato, petunia, soybean, cotton and Lesquerella fendleri (Brassicaceae). In this study we demonstrate a polyethylene glycol-mediated plastid transformation system for lettuce that generates fertile, homoplasmic, plastid-transformed lines. Transformation was achieved using a vector that targets genes to the trnA/trnI intergenic region of the lettuce plastid genome employing the aadA gene as a selectable marker against spectinomycin. Spectinomycin resistance and heterologous gene transcription were shown in T(1) plants derived from self-pollinated primary regenerants demonstrating transmission of the plastid-encoded transgene to the first seed generation. Crossing with male sterile wild-type lettuce showed that spectinomycin resistance was not transmitted via pollen. Constructs containing the gfp gene showed plastid-based expression of green fluorescent protein. The lettuce plastid could have potential both as a production and a delivery system for edible human therapeutic proteins.

Published
2005

16. Reverse breeding: a novel breeding approach based on engineered meiosis

Author

Dirks, Rob, primary, van Dun, Kees, additional, de Snoo, C. Bastiaan, additional, van den Berg, Mark, additional, Lelivelt, Cilia L. C., additional, Voermans, William, additional, Woudenberg, Leo, additional, de Wit, Jack P. C., additional, Reinink, Kees, additional, Schut, Johan W., additional, van der Zeeuw, Eveline, additional, Vogelaar, Aat, additional, Freymark, Gerald, additional, Gutteling, Evert W., additional, Keppel, Marina N., additional, van Drongelen, Paul, additional, Kieny, Matthieu, additional, Ellul, Philippe, additional, Touraev, Alisher, additional, Ma, Hong, additional, de Jong, Hans, additional, and Wijnker, Erik, additional

Published
2009
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17. Plastid transformation in lettuce (Lactuca sativa L.) by polyethylene glycol treatment of protoplasts.

Author

Lelivelt CL, van Dun KM, de Snoo CB, McCabe MS, Hogg BV, and Nugent JM

Subjects
Anti-Bacterial Agents pharmacology, Cells, Cultured, DNA, Intergenic genetics, Drug Resistance genetics, Genetic Vectors, Lactuca enzymology, Nucleotidyltransferases genetics, Plant Leaves genetics, Plants, Genetically Modified genetics, Protoplasts cytology, Spectinomycin pharmacology, Surface-Active Agents pharmacology, Transgenes genetics, Chloroplasts genetics, Lactuca genetics, Polyethylene Glycols pharmacology, Transfection methods, Transformation, Genetic
Abstract

A detailed protocol for PEG-mediated plastid transformation of Lactuca sativa cv. Flora, using leaf protoplasts, is described. Successful plastid transformation using protoplasts requires a large number of viable cells, high plating densities, and an efficient regeneration system. Transformation was achieved using a vector that targets genes to the trnI/trnA intergenic region of the lettuce plastid genome. The aadA gene, encoding an adenylyltransferase enzyme that confers spectinomycin resistance, was used as a selectable marker. With the current method, the expected transformation frequency is 1-2 spectinomycin-resistant cell lines per 10(6) viable protoplasts. Fertile, diploid, homoplasmic, plastid-transformed lines were obtained. Transmission of the plastid-encoded transgene to the T1 generation was demonstrated.

Published
2014
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