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Osteological data, such as biological sex, constitute a base for research in paleodemography and palaeopathology, as well as for understanding past socio-cultural practices. Despite extensive research efforts concerning cremated human remains over the past decades, an internationally acknowledged, standardized osteological protocol is not fully agreed upon. Furthermore, assessing cremation research practices from the literature is challenging because analysis reports are often written in the national languages of practitioners, which makes them difficult to access by an international audience. This study addresses this gap by directly reaching out to experts working with cremated human remains through an online questionnaire in Lime Survey. The aim is to identify and characterize patterns in cremation analysis practices. A particular emphasis was put on the methods of biological sex estimation. While the significance of these data is widely acknowledged, the ways in which they are obtained in practice are seldom examined. The results of this survey reveal an absence of standardization in protocols of analysis, and data collection, as well as in reporting on the cremated remains in publications and reports. Notably, the findings reveal regional preferences in methodological choices and inconsistent institutional training. A majority of respondents expressed a preference for traditional morphological methods over recently published metric and alternative methods for sex estimation. These variations underscore the complexity of establishing standardized cremation analysis protocols and highlight the importance of considering regional contexts and training in shaping future research practices. [ABSTRACT FROM AUTHOR]

SUMMARY: The circadian clock organizes physiological processes in plants to occur at specific times of the day, optimizing efficient use of resources. Nitrate is a crucial inorganic nitrogen source for agricultural systems to sustain crop productivity. However, because nitrate fertilization has a negative impact on the environment, it is important to carefully manage nitrate levels. Understanding crop biological rhythms can lead to more ecologically friendly agricultural practices. Gating responses through the circadian clock could be a strategy to enhance root nitrate uptake and to limit nitrate runoff. In Arabidopsis, the NITRATE TRANSPORTER 2.1 (NRT2.1) gene encodes a key component of the high‐affinity nitrate transporter system. Our study reveals that NRT2.1 exhibits a rhythmic expression pattern, with daytime increases and nighttime decreases. The NRT2.1 promoter activity remains rhythmic under constant light, indicating a circadian regulation. The clock‐associated transcription factor LUX ARRHYTHMO (LUX) binds to the NRT2.1 promoter in vivo. Loss‐of‐function of LUX leads to increased NRT2.1 transcript levels and root nitrate uptake at dusk. This supports LUX acting as a transcriptional repressor and modulating NRT2.1 expression in a time‐dependent manner. Furthermore, applying nitrate at different times of the day results in varying magnitudes of the transcriptional response in nitrate‐regulated genes. We also demonstrate that a defect in the high‐affinity nitrate transport system feeds back to the central oscillator by modifying the LUX promoter activity. In conclusion, this study uncovers a molecular pathway connecting the root nitrate uptake and circadian clock, with potential agro‐chronobiological applications. Significance Statement: This study highlights the connection between the circadian clock and nitrate high‐affinity transport in plant roots, with potential applications in agro‐chronobiology. The evening clock‐associated transcription factor LUX ARRHYTHMO controls the rhythmic expression pattern of NITRATE TRANSPORTER 2.1. [ABSTRACT FROM AUTHOR]

The site of San Valentino in San Vito al Tagliamento is one of the main urnfield cemeteries in northeastern Italy. Archaeological excavations carried out in the seventies brought to light a cremation cemetery consisting of mainly urn graves with pottery and metal artefacts as grave goods. These materials suggest that the individuals buried in San Valentino were not an isolated local community but had intense contacts with other north-Adriatic communities, in particular with the neighbouring Veneto area, as suggested by the close similarity of the biconical vessels with those recovered in the graves of Este. This paper provides the first osteological study of a preserved sample of individuals buried at San Valentino and uses an innovative multi-proxy approach to refine the chronology of the site through radiocarbon dating of bone apatite, investigate human mobility using strontium isotopes on calcined human remains, and reconstruct the funerary practices by combining FTIR-ATR data with carbon and oxygen isotope ratios on cremated bones. The results date the cemetery to the end of the Late Bronze Age and the Early Iron Age, with a sporadic occupation in the fourth century BC. Strontium isotopes and concentrations show the analysed individuals buried at San Valentino were a local community that exploited nearby food resources. Interestingly, variations in cremation conditions were detected between San Valentino and the contemporary sites of Velzeke, Blicquy, Grand Bois, and Herstal, located in Belgium, by using FTIR-ATR and carbon and oxygen isotope data. This multi-proxy approach applied to the study of cremated human remains can open new research possibilities, being potentially extendable to the study of many pre- and proto-historic and historic communities that practised cremation. [ABSTRACT FROM AUTHOR]

Cremation graves appear in different forms and shapes, from urns to simple pits and from single to plural graves. The challenging nature of highly fragmented cremated human remains renders the identification of multiple individuals within the same cremation grave rather complex. Osteological analyses alone are often insufficient to detect the presence of bone fragments from different individuals as they are small and diagnostic elements are often missing, although, detection of nonadult bone fragments within adult bone assemblages (or the other way around) points to the presence of at least two individuals—one adult and one nonadult—within the same grave. The combination of osteological analyses, radiocarbon dating, and strontium isotope ratios has proven to be particularly powerful. At different Belgian Metal Age sites, this novel multi-disciplinary approach enabled to identify the presence of bone fragments belonging to up to three different individuals within the same cremation grave who were cremated up to several centuries apart. Whether the presence of these two or three individuals in the same grave is intentional (e.g. curation) or not requires more in-depth analyses. This study shows the high level of complexity of cremation burial (intentionally or not) and shows the necessity to carry out all analytical measurements (i.e. radiocarbon dating, infrared, elemental and isotope analyses) on the same bone fragment to ensure the results are related to the same individual. [ABSTRACT FROM AUTHOR]