1. Resistance of Gram-positive bacteria to nisin is not determined by Lipid II levels
- Author
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Kramer, NE, Smid, EJ, Kok, J, de Kruijff, B, Kuipers, OP, Breukink, E, Kramer, Naomi E., Smid, Eddy J., Groningen Biomolecular Sciences and Biotechnology, Molecular Genetics, and Faculty of Science and Engineering
- Subjects
Gram-positive bacteria ,Microbial Sensitivity Tests ,Gram-Positive Bacteria ,medicine.disease_cause ,Microbiology ,Micrococcus ,resistance ,chemistry.chemical_compound ,Polyisoprenyl Phosphates ,Listeria monocytogenes ,Bacteriocin ,Drug Resistance, Bacterial ,BINDING ,Genetics ,medicine ,polycyclic compounds ,BIOSYNTHESIS ,LISTERIA-MONOCYTOGENES ,Molecular Biology ,Nisin ,biology ,Lipid II ,food and beverages ,Lantibiotics ,biochemical phenomena, metabolism, and nutrition ,Streptococcaceae ,biology.organism_classification ,PEPTIDOGLYCAN ,Uridine Diphosphate N-Acetylmuramic Acid ,Anti-Bacterial Agents ,LANTIBIOTICS ,chemistry ,bactoprenyl-phosphate ,bacteria ,lipids (amino acids, peptides, and proteins) ,nisin ,SENSITIVITY ,MEMBRANE ,Bacteria - Abstract
Lipid II is essential for nisin-mediated pore formation at nano-molar concentrations. We tested whether nisin resistance could result from different Lipid II levels, by comparing the maximal Lipid II pool in Micrococcus flavus (sensitive) and Listeria monocytogenes (relatively insensitive) and their nisin-resistant variants, with a newly developed method. No correlation was observed between the maximal Lipid II pool and nisin sensitivity, as was further corroborated by using spheroplasts of nisin-resistant and wild-type strains of M. flavus, which were equally sensitive to nisin. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
- Published
- 2004