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8 results on '"Smeehuijzen, Lisa"'

1. Immunometabolic Signatures of Circulating Monocytes in Humans with Obesity and Insulin Resistance

Author

Smeehuijzen, Lisa, primary, Gijbels, Anouk, additional, Nugteren-Boogaard, Joline P., additional, Vrieling, Frank, additional, Boudjadja, Mehdi Boutagouga, additional, Trouwborst, Inez, additional, Jardon, Kelly M., additional, Hul, Gabby B., additional, Feskens, Edith J.M., additional, Blaak, Ellen E., additional, Goossens, Gijs H., additional, Afman, Lydia A., additional, and Stienstra, Rinke, additional

Published
2024
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3. Triglyceride breakdown from lipid droplets regulates the inflammatory response in macrophages

Author

Dierendonck, Xanthe A.M.H. van, Vrieling, Frank, Smeehuijzen, Lisa, Deng, Lei, Boogaard, Joline P., Croes, Cresci-Anne, Kersten, Sander, Stienstra, R., Dierendonck, Xanthe A.M.H. van, Vrieling, Frank, Smeehuijzen, Lisa, Deng, Lei, Boogaard, Joline P., Croes, Cresci-Anne, Kersten, Sander, and Stienstra, R.

Abstract

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Published
2022

4. Triglyceride breakdown from lipid droplets regulates the inflammatory response in macrophages

Author

van Dierendonck, Xanthe A.M.H., Vrieling, Frank, Smeehuijzen, Lisa, Deng, Lei, Boogaard, Joline P., Croes, Cresci Anne, Temmerman, Lieve, Wetzels, Suzan, Biessen, Erik, Kersten, Sander, Stienstra, Rinke, van Dierendonck, Xanthe A.M.H., Vrieling, Frank, Smeehuijzen, Lisa, Deng, Lei, Boogaard, Joline P., Croes, Cresci Anne, Temmerman, Lieve, Wetzels, Suzan, Biessen, Erik, Kersten, Sander, and Stienstra, Rinke

Abstract

In response to inflammatory activation by pathogens, macrophages accumulate triglycerides in intracellular lipid droplets. The mechanisms underlying triglyceride accumulation and its exact role in the inflammatory response of macrophages are not fully understood. Here, we aim to further elucidate the mechanism and function of triglyceride accumulation in the inflammatory response of activated macrophages. Lipopolysaccharide (LPS)-mediated activation markedly increased triglyceride accumulation in macrophages. This increase could be attributed to up-regulation of the hypoxia-inducible lipid droplet–associated (HILPDA) protein, which down-regulated adipose triglyceride lipase (ATGL) protein levels, in turn leading to decreased ATGL-mediated triglyceride hydrolysis. The reduction in ATGL-mediated lipolysis attenuated the inflammatory response in macrophages after ex vivo and in vitro activation, and was accompanied by decreased production of prostaglandin-E2 (PGE2) and interleukin-6 (IL-6). Overall, we provide evidence that LPS-mediated activation of macrophages suppresses lipolysis via induction of HILPDA, thereby reducing the availability of proinflammatory lipid precursors and suppressing the production of PGE2 and IL-6.

Published
2022

6. CENCAT enables immunometabolic profiling by measuring protein synthesis via bioorthogonal noncanonical amino acid tagging

Author

Vrieling, Frank, van der Zande, Hendrik J.P., Naus, Britta, Smeehuijzen, Lisa, van Heck, Julia I.P., Ignacio, Bob J., Bonger, Kimberly M., Van den Bossche, Jan, Kersten, Sander, and Stienstra, Rinke

Abstract

Cellular energy metabolism significantly contributes to immune cell function. To further advance immunometabolic research, novel methods to study the metabolism of immune cells in complex samples are required. Here, we introduce CENCAT (cellular energetics through noncanonical amino acid tagging). This technique utilizes click labeling of alkyne-bearing noncanonical amino acids to measure protein synthesis inhibition as a proxy for metabolic activity. CENCAT successfully reproduced known metabolic signatures of lipopolysaccharide (LPS)/interferon (IFN)γ and interleukin (IL)-4 activation in human primary macrophages. Application of CENCAT in peripheral blood mononuclear cells revealed diverse metabolic rewiring upon stimulation with different activators. Finally, CENCAT was used to analyze the cellular metabolism of murine tissue-resident immune cells from various organs. Tissue-specific clustering was observed based on metabolic profiles, likely driven by microenvironmental priming. In conclusion, CENCAT offers valuable insights into immune cell metabolic responses, presenting a powerful platform for studying cellular metabolism in complex samples and tissues in both humans and mice.

Published
2024
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