Search

Your search keyword '"Small, Pamela"' showing total 210 results
Start Over Author "Small, Pamela"
210 results on '"Small, Pamela"'

1. Praxis

Author

St. John, Edward P., primary, Anderson, Bart, additional, Chapin-Kjelstrom, Judith, additional, Fisher, Amy S., additional, Felder-Small, Pamela, additional, and Gasman, Marybeth, additional

Published
2022
Full Text
View/download PDF

2. Sankofa: African American Perspectives on Race and Culture in US Doctoral Education

Author

Small, Pamela Felder, Barker, Marco J., Gasman, Marybeth, Small, Pamela Felder, Barker, Marco J., and Gasman, Marybeth

Abstract

"Sankofa" reexamines doctoral education through the lens of African American and Black experiences. Drawing on the African diasporic legacy of "Sankofa" and the notion that "it is not wrong to go back for that which you have forgotten," the contributors "go back" to address legacies of exclusion in higher education and take care to center and honor the contributions of historically marginalized doctoral students. Whereas earlier studies focused largely on socialization, departmental norms, and statistical portraits of doctoral degree attachment, this book illuminates the ways African American students encounter, navigate, and make sense of their doctoral experiences and especially the impact of race and culture on those experiences. Individual chapters look at STEM programs, the intersections of race and gender, the role of HBCUs, and students' relationships with faculty and advisors. Amid growing diversity across programs and institutions, "Sankofa" provides a critical model for applying culturally based frameworks in educational research, as well as practical strategies for better understanding and responding to the needs of students of color in predominantly White contexts.

Published
2020

19. Identification of three cytotoxic early proteins of mycobacteriophage L5 leading to growth inhibition in Mycobacterium smegmatis

Author

Rybniker, Jan, Plum, Georg, Robinson, Nirmal, Small, Pamela L., and Hartmann, Pia

Subjects
Bacteriophages -- Physiological aspects, Bacteriophages -- Genetic aspects, Bacteriophages -- Research, Mycobacteria -- Physiological aspects, Mycobacteria -- Research, Mycobacterium -- Physiological aspects, Mycobacterium -- Research, Proteins -- Identification and classification, Proteins -- Research, Biological sciences
Abstract

Mycobacteriophage L5 is a temperate phage with a broad host range among the fast- and slow-growing mycobacteria such as Mycobacterium smegmatis, Mycobacterium tuberculosis, Mycobacterium avium and Mycobacterium ulcerans. L5 switches off host protein synthesis during the early stage of lytic growth, as was previously shown by protein expression profiling. Also, lethal genetic elements have been identified in L5 based on the fact that transformants could not be obtained with these genes. Using an inducible mycobacterial shuttle vector, we have identified three ORFs within an early operon of mycobacteriophage L5 which encode gene products (gp) toxic to the host M. smegmatis when expressed. These ORFs, coding for gp77, gp78 and gp79, presumably function as shut-off genes during early stages of phage replication. There is evidence that cell division is affected by one of the proteins (gp79). The transcription of the cytotoxic polypeptides is directed by a promoter situated in ORF83 and transcription control is achieved through the phage repressor gp71, which is shown by co-expression of this protein. The findings presented here should provide useful tools for the molecular genetics of mycobacteria. Further analysis of these and other mycobacteriophage-derived toxic polypeptides, together with the identification of their cellular targets, might provide a tool for the rapid identification of promising drug targets in emerging and re-emerging mycobacterial pathogens.

Published
2008

20. Giant plasmid-encoded polyketide synthases produce the macrolide toxin of Mycobacterium ulcerans

Author

Stinear, Timothy P., Mve-Obiang, Armand, Small, Pamela L.C., Frigui, Wafa, Pryor, Melinda J., Brosch, Roland, Jenkin, Grant A., Johnson, Paul D.R., Davies, John K., Lee, Richard E., Adusumilli, Sarojini, Garnier, Thierry, Haydock, Stephen F., Leadlay, Peter F., and Cole, Stewart T.

Subjects
Polyketides -- Research, Science and technology
Abstract

Mycobacterium ulcerans (MU), an emerging human pathogen harbored by aquatic insects, is the causative agent of Buruli ulcer, a devastating skin disease rife throughout Central and West Africa. Mycolactone, an unusual macrolide with cytotoxic and immunosuppressive properties, is responsible for the massive s.c. tissue destruction seen in Buruli ulcer. Here, we show that MU contains a 174-kb plasmid, pMUM001, bearing a cluster of genes encoding giant polyketide synthases (PKSs), and polyketide-modifying enzymes, and demonstrate that these are necessary and sufficient for mycolactone synthesis. This is a previously uncharacterized example of plasmid-mediated virulence in a Mycobacterium, and the emergence of MU as a pathogen most likely reflects the acquisition of pMUM001 by horizontal transfer. The 12-membered core of mycolactone is produced by two giant, modular PKSs, MLSA1 (1.8 MDa) and MLSA2 (0.26 MDa), whereas its side chain is synthesized by MLSB (1.2 MDa), a third modular PKS highly related to MLSA1. There is an extreme level of sequence identity within the different domains of the MLS cluster (>97% amino acid identity), so much so that the 16 ketosynthase domains seem functionally identical. This is a finding of significant consequence for our understanding of polyketide biochemistry. Such detailed knowledge of mycolactone will further the investigation of its mode of action and the development of urgently needed therapeutic strategies to combat Buruli ulcer.

Published
2004

21. Antifungal activity of two oxadiazole compounds for the paracoccidioidomycosis treatment

Author

Rodrigues-Vendramini, Franciele Abigail Vilugron, Faria, Daniella Renata, Arita, Glaucia Sayuri, Capoci, Isis Regina Grenier, Sakita, Karina Mayumi, Caparroz-Assef, Silvana Martins, Becker, Tania Cristina Alexandrino, de Souza Bonfim-Mendonça, Patrícia, Felipe, Maria Sueli, Svidzinski, Terezinha Inez Estivalet, Maigret, Bernard, Kioshima, Erika Seki, Small, Pamela, Universidade Estadual de Maringá [Maringá] (UEM), University of Brasilia [Brazil] (UnB), Computational Algorithms for Protein Structures and Interactions (CAPSID), Inria Nancy - Grand Est, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Department of Complex Systems, Artificial Intelligence & Robotics (LORIA - AIS), Laboratoire Lorrain de Recherche en Informatique et ses Applications (LORIA), Institut National de Recherche en Informatique et en Automatique (Inria)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche en Informatique et en Automatique (Inria)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Laboratoire Lorrain de Recherche en Informatique et ses Applications (LORIA), Institut National de Recherche en Informatique et en Automatique (Inria)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Department of Cellular Biology [Brasilia], Universidade de Brasilia [Brasília] (UnB), and The University of Tennessee [Knoxville]

Subjects
Male, 0301 basic medicine, Fungal Structure, Antifungal Agents, RC955-962, Colony Count, Microbial, Pharmacology, Pathology and Laboratory Medicine, Toxicology, Paracoccidioides, chemistry.chemical_compound, Fluorescence Microscopy, 0302 clinical medicine, Arctic medicine. Tropical medicine, Medicine and Health Sciences, Immune Response, Lung, Fungicides, Microscopy, Mice, Inbred BALB C, Oxadiazoles, Antimicrobials, Histocytochemistry, Paracoccidioidomycosis, Histopathological analysis, Fungal Diseases, Drugs, Light Microscopy, Eukaryota, Agriculture, Drug Synergism, 3. Good health, Fungicide, Treatment Outcome, Infectious Diseases, Toxicity, Public aspects of medicine, RA1-1270, Agrochemicals, Research Article, Neglected Tropical Diseases, Antifungal, medicine.drug_class, Immunology, 030231 tropical medicine, Oxadiazole, Mycology, Microbial Sensitivity Tests, Research and Analysis Methods, Microbiology, 03 medical and health sciences, Signs and Symptoms, Pharmacotherapy, Diagnostic Medicine, Microbial Control, Amphotericin B, medicine, Animals, Inflammation, Antifungals, Microbial Viability, Organisms, Fungi, Public Health, Environmental and Occupational Health, Biology and Life Sciences, Tropical Diseases, medicine.disease, Disease Models, Animal, 030104 developmental biology, chemistry, [INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM]
Abstract

Paracoccidioidomycosis (PCM) is a neglected disease present in Latin America with difficulty in treatment and occurrence of serious sequelae. Thus, the development of alternative therapies is imperative. In the current work, two oxadiazole compounds (LMM5 and LMM11) presented fungicidal activity against Paracoccidioides spp. The minimum inhibitory and fungicidal concentration values ranged from 1 to 32 μg/mL, and a synergic effect was observed for both compounds when combined with Amphotericin B. LMM5 and LMM11 were able to reduce CFU counts (≥2 log10) on the 5th and 7th days of time-kill curve, respectively. The fungicide effect was confirmed by fluorescence microscopy (FUN-1/FUN-2). The hippocratic screening and biochemical analysis were performed in Balb/c male mice that received a high dose of each compound, and the compounds showed no in vivo toxicity. The treatment of experimental PCM with the new oxadiazoles led to significant reduction in CFU (≥1 log10). Histopathological analysis of the groups treated exhibited control of inflammation, as well as preserved lung areas. These findings suggest that LMM5 and LMM11 are promising hits structures, opening the door for implementing new PCM therapies., Author summary Paracoccidioidomycosis (PCM) is a granulomatous fungal infection with clinically severe forms and serious pulmonary sequelae. The current limited arsenal and prolonged treatment regimen demonstrate the need for new antifungals. This study reveals two fungicidal oxadiazole compounds for PCM treatment. The in vitro assay showed the antifungal activity and the synergic effect with Amphotericin B. The high doses administration in mice showed absence of toxicity, which allowed to demonstrate the in vivo antifungal activity.

Published
2019

25. Interaction of Mycobacterium ulcerans with mosquito species: implications for transmission and trophic relationships

Author

Wallace, John R., Gordon, Matthew C., Hartsell, Lindsey, Mosi, Lydia, Benbow, M. Eric, Merritt, Richard W., and Small, Pamela L.C.

Subjects
Mycobacteria -- Environmental aspects, Mycobacteria -- Research, Mycobacterium -- Environmental aspects, Mycobacterium -- Research, Buruli ulcer -- Environmental aspects, Buruli ulcer -- Research, Biological sciences
Abstract

A study was conducted to examine the potential of mosquitoes to serve as biological or mechanical vectors or as environmental reservoirs for Mycobacterium ulcerans which is the causative agent of Buruli ulcer, a severe necrotizing skin disease. The ingested wild-type M. ulcerans ingested by the mosquitoes larvae which remain infected throughout larval development but not carried over into the pupae or adult mosquitoes indicated an unlikely role for mosquitoes as biological vectors.

Published
2010

26. Large sequence polymorphisms unveil the phylogenetic relationship of environmental and pathogenic mycobacteria related to Mycobacterium ulcerans

Author

Kaser, Michael, Hauser, Julia, Small, Pamela, and Pluschke, Gerd

Subjects
Cladistic analysis -- Usage, DNA testing -- Analysis, Genetic polymorphisms -- Analysis, Mycobacteria -- Genetic aspects, Mycobacteria -- Physiological aspects, Mycobacterium -- Genetic aspects, Mycobacterium -- Physiological aspects, Biological sciences
Abstract

The genetic fingerprinting analyses have helped in disclosing the phylogenetic relatedness and the dispersal between environmental and pathogenic mycolactone producers and the identification of genetic prerequisites that has enabled lateral gene transfer of such plasmids. Dynamic chromosomal loci identified in Mycobacterium ulcerans is used for characterizing large sequence polymorphisms for the phylogenetic analysis of mycolactone-producing mycobacteria (MPMs).

Published
2009

27. Landscape and environmental influences on Mycobacterium ulcerans distribution among aquatic sites in Ghana

Author

Pileggi, Shannon M., primary, Jordan, Heather, additional, Clennon, Julie A., additional, Whitney, Ellen, additional, Benbow, M. Eric, additional, Merritt, Richard, additional, McIntosh, Mollie, additional, Kimbirauskas, Ryan, additional, Small, Pamela, additional, Boakye, Daniel, additional, Quaye, Charles, additional, Qi, Jiaguo, additional, Campbell, Lindsay, additional, Gronseth, Jenni, additional, Ampadu, Edwin, additional, Opare, William, additional, and Waller, Lance A., additional

Published
2017
Full Text
View/download PDF

28. Insights from the complete genome sequence of Mycobacterium marinum on the evolution of Mycobacterium tuberculosis

Author

Stinear, Timothy P., Seemann, Torsten, Harrison, Paul F., Jenkin, Grant A., Davies, John K., Johnson, Paul D.R., Abdellah, Zahra, Arrowsmith, Claire, Chillingworth, Tracey, Churcher, Carol, Clarke, Kay, Cronin, Ann, Davis, Paul, Goodhead, Ian, Holroyd, Nancy, Jagels, Kay, Lord, Angela, Moule, Sharon, Mungall, Karen, Norbertczak, Halina, Quail, Michael A., Rabbinowitsch, Ester, Walker, Danielle, White, Brian, Whitehead, Sally, Small, Pamela L.C., Brosch, Roland, Ramakrishnan, Lalita, Fischbach, Michael A., Parkhill, Julian, and Cole, Stewart T.

Subjects
Genomics -- Analysis, Mycobacteria -- Genetic aspects, Mycobacterium -- Genetic aspects, Tuberculosis -- Genetic aspects, Tuberculosis -- Causes of, Health
Abstract

The genome sequence of Mycobacterium marnium is determined and it's very close genetic relationship with Mycobacterium tuberculosis is exploited to provide further insight into the multiple changes that shapes Mycobacterium tuberculosis into a major human pathogen from environmental origins. The study provides a foundation for using Mycobacterium marinum to better understand the determinants of pathogenesis of tuberculosis.

Published
2008

29. Reductive evolution and niche adaptation inferred from the genome of Mycobacterium ulcerans, the causative agent of Buruli ulcer

Author

Stinear, Timothy P., Seemann, Torsten, Frigui, Wafa; Reysset, Gilles, Garnier, Thierry, Meurice, Guillaume, Simon, David, Pidot, Sacha, Bouchier, Christiane, Tichit, Magali, Porter, Jessica L., Ryan, Janine, Ma, Laurence, Johnson, Paul D.R., Jenkin, Grant A., Davies, John K., Small, Pamela L.C., and Jones, Louis M.

Subjects
Mycobacteria -- Genetic aspects, Mycobacteria -- Physiological aspects, Mycobacterium -- Genetic aspects, Mycobacterium -- Physiological aspects, Nucleotide sequence -- Research, Health
Abstract

A complete 5.8-Mb genome sequence of Mycobacterium ulcerans, which causes Buruli ulcer in humans, is described, showing that it comprises two circular replicons, a chromosome of 5632 kb and a virulence plasmid of 174 kb. The data indicate that M. ulcerans has evolved via lateral gene transfer and reductive evolution from the generalist, more rapid-growing environmental species M. marinum to become a niche-adapted specialist.

Published
2007

31. Comparative Sigma Factor-mRNA Levels in Mycobacterium marinum under Stress Conditions and during Host Infection

Author

Pettersson, B. M. Fredrik, primary, Das, Sarbashis, additional, Behra, Phani Rama Krishna, additional, Jordan, Heather R., additional, Ramesh, Malavika, additional, Mallick, Amrita, additional, Root, Kate M., additional, Cheramie, Martin N., additional, de la Cruz Melara, Irma, additional, Small, Pamela L. C., additional, Dasgupta, Santanu, additional, Ennis, Don G., additional, and Kirsebom, Leif A., additional

Published
2015
Full Text
View/download PDF

39. LETTERS

Author

Jordan, Kim, Beltrami, John, Finn, Linda, Small, Pamela, Tips, Nancy, Smith, Pamela, Jackson, Charles, Mccarsky, Drew, Martin, James, Thomas, Pat, Berger, Corey, Honicker, Dolph, Lerner, Jonathan, Sandin, Tammy, Allen, Jim, Weil, Carole, Wagner, Debbie, and Frasier, Brian

Subjects
General interest, News, opinion and commentary
Abstract

Dove shoot item a joke, right? Is it possible that the item about a dove shoot to raise funds for Karen Floyd, South Carolina's Republican nominee for education superintendent, is [...]

Published
2006

50. Distribution of Mycobacterium ulcerans in Buruli Ulcer Endemic and Non-Endemic Aquatic Sites in Ghana

Author

Williamson, Heather R., primary, Benbow, Mark E., additional, Nguyen, Khoa D., additional, Beachboard, Dia C., additional, Kimbirauskas, Ryan K., additional, McIntosh, Mollie D., additional, Quaye, Charles, additional, Ampadu, Edwin O., additional, Boakye, Daniel, additional, Merritt, Richard W., additional, and Small, Pamela L. C., additional

Published
2008
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results