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156 results on '"Slooff, Maarten J.H."'

2. LPS-induced downregulation of MRP2 and BSEP in human liver is due to a posttranscriptional process

Author

Elferink, Marieke G.L., Olinga, Peter, Draaisma, Annelies L., Merema, Marjolijn T., Faber, Klaas Nico, Slooff, Maarten J.H., Meijer, Dirk K.F., and Groothuis, Geny M.M.

Subjects
Cytokines -- Research, Liver -- Research, Biological sciences
Abstract

LPS-induced downregulation of MRP2 and BSEP in human liver is due to a posttranscriptional process. Am J Physiol Gastrointest Liver Physiol 287: G1008-G1016, 2004. First published June 17, 2004; doi: 10.1152/ajpgi.00071.2004.--Endotoxin-induced cholestasis in rodents is caused by hepatic downregulation of transporters, including the basolateral [Na.sup.+]-dependent taurocholate transporter (ntcp) and the canalicular bile salt export pump (bsep) and multidrug resistance-associated protein 2 (mrp2). Details about the regulation of the human transporter proteins during this process are lacking. We used precision-cut human and rat liver slices to study the regulation of transporter expression during LPS-induced cholestasis. We investigated the effect of LPS on nitrate/nitrite and cytokine production in relation to the expression of inducible nitric oxide synthase, NTCP, BSEP, and MRP2 both at the level of mRNA with RT-PCR and protein using immunofluorescence microscopy. In liver slices from both species, LPS-induced expression of inducible nitric oxide synthase was detected within 1-3 h and remained increased over 24 h. In rat liver slices, this was accompanied by a significant decrease of rat ntcp and mrp2 mRNA levels, whereas bsep levels were not affected. These results are in line with previous in vivo studies and validate our liver slice technique. In LPS-treated human liver slices, NTCP mRNA was downregulated and showed an inverse correlation with the amounts of TNF-[alpha] and Il-1[beta] produced. In contrast, MRP2 and BSEP mRNA levels were not affected under these conditions. However, after 24-h LPS challenge, both proteins were virtually absent in human liver slices, whereas marker proteins remained detectable. In conclusion, we show that posttranscriptional mechanisms play a more prominent role in LPS-induced regulation of human MRP2 and BSEP compared with the rat transporter proteins. human liver slices; endotoxin-induced cholestasis, transporter expression; cytokines; immunofluorescence microscopy

Published
2004

34. Expression of Heme Oxygenase‐1 in Human Livers Before Transplantation Correlates with Graft Injury and Function After Transplantation

Author

Geuken, Erwin, primary, Buis, Carlijn I., additional, Visser, Dorien S., additional, Blokzijl, Hans, additional, Moshage, Han, additional, Nemes, Balazs, additional, Leuvenink, Henri G.D., additional, De Jong, Koert P., additional, Peeters, Paul M.J.G., additional, Slooff, Maarten J.H., additional, and Porte, Robert J., additional

Published
2005
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45. DONOR-SPECIFIC HYPOREACTIVITY AFTER LIVER TRANSPLANTATION

Author

de Haan, Aalzen, primary, van den Berg, Arie P., additional, Hepkema, Bouke G., additional, van Dijk, Elinda, additional, Haagsma, Elizabeth B., additional, The, T. Hauw, additional, Slooff, Maarten J.H., additional, Lems, Simon P.M., additional, de Leij, Lou F.M.H., additional, and Prop, Jochum, additional

Published
1998
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47. Splenic artery aneurysms in liver transplant patients

Author

Kóbori, László, primary, van der Kolk, Marjan J., additional, de Jong, Koert P., additional, Peeters, Paul M.J.G., additional, Klompmaker, Ids J., additional, Kok, Theo, additional, Haagsma, Elizabeth B., additional, and Slooff, Maarten J.H., additional

Published
1997
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