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3. Contralateral second dose improves antibody responses to a 2-dose mRNA vaccination regimen

Author

Fazli, Sedigheh, Thomas, Archana, Estrada, Abram E., Ross, Hiro A.P., Lee, David Xthona, Kazmierczak, Steven, Slifka, Mark K., Montefiori, David, Messer, William B., and Curlin, Marcel E.

Subjects
Promega Corp., Vaccination -- Health aspects, Biotechnology industry -- Health aspects, Messenger RNA -- Health aspects, B cells -- Health aspects, Immunoglobulin G -- Health aspects, Health care industry
Abstract

BACKGROUND. Vaccination is typically administered without regard to site of prior vaccination, but this factor may substantially affect downstream immune responses. METHODS. We assessed serological responses to initial COVID-19 vaccination in baseline seronegative adults who received second-dose boosters in the ipsilateral or contralateral arm relative to initial vaccination. We measured serum SARSCoV-2 spike-specific Ig, receptor-binding domain-specific (RBD-specific) IgG, SARS-CoV-2 nucleocapsid-specific IgG, and neutralizing antibody titers against SARS-CoV-2.D614G (early strain) and SARS-CoV-2.B.1.1.529 (Omicron) at approximately 0.6, 8, and 14 months after boosting. RESULTS. In 947 individuals, contralateral boosting was associated with higher spike-specific serum Ig, and this effect increased over time, from a 1.1-fold to a 1.4-fold increase by 14 months (P < 0.001). A similar pattern was seen for RBD-specific IgG. Among 54 pairs matched for age, sex, and relevant time intervals, arm groups had similar antibody levels at study visit 2 (W2), but contralateral boosting resulted in significantly higher binding and neutralizing antibody titers at W3 and W4, with progressive increase over time, ranging from 1.3-fold (total Ig, P = 0.007) to 4.0-fold (pseudovirus neutralization to B.1.1.529, P < 0.001). CONCLUSIONS. In previously unexposed adults receiving an initial vaccine series with the BNT162b2 mRNA COVID-19 vaccine, contralateral boosting substantially increases antibody magnitude and breadth at times beyond 3 weeks after vaccination. This effect should be considered during arm selection in the context of multidose vaccine regimens. FUNDING. M.J. Murdock Charitable Trust, OHSU Foundation, NIH., Introduction In the 227 years since Edward Jenner demonstrated that inoculation with material from cowpox lesions could provide protection from symptomatic illness due to smallpox, vaccine research has advanced to [...]

Published
2024
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4. Polygenic prediction of atopic dermatitis improves with atopic training and filaggrin factors

Author

Arehart, Christopher H, Daya, Michelle, Campbell, Monica, Boorgula, Meher Preethi, Rafaels, Nicholas, Chavan, Sameer, David, Gloria, Hanifin, Jon, Slifka, Mark K, Gallo, Richard L, Hata, Tissa, Schneider, Lynda C, Paller, Amy S, Ong, Peck Y, Spergel, Jonathan M, Guttman-Yassky, Emma, Leung, Donald YM, Beck, Lisa A, Gignoux, Christopher R, Mathias, Rasika A, and Barnes, Kathleen C

Subjects
Biomedical and Clinical Sciences, Immunology, Genetics, Prevention, Clinical Research, Human Genome, Aetiology, 2.1 Biological and endogenous factors, Dermatitis, Atopic, Female, Filaggrin Proteins, Genetic Predisposition to Disease, Genome-Wide Association Study, Humans, Infant, Linkage Disequilibrium, Loss of Function Mutation, Male, Phenotype, Atopic dermatitis, polygenic risk score, atopic march, allergic disease, genetic architecture, filaggrin, disease prediction, genetic predisposition, Allergy
Abstract

BackgroundWhile numerous genetic loci associated with atopic dermatitis (AD) have been discovered, to date, work leveraging the combined burden of AD risk variants across the genome to predict disease risk has been limited.ObjectivesThis study aims to determine whether polygenic risk scores (PRSs) relying on genetic determinants for AD provide useful predictions for disease occurrence and severity. It also explicitly tests the value of including genome-wide association studies of related allergic phenotypes and known FLG loss-of-function (LOF) variants.MethodsAD PRSs were constructed for 1619 European American individuals from the Atopic Dermatitis Research Network using an AD training dataset and an atopic training dataset including AD, childhood onset asthma, and general allergy. Additionally, whole genome sequencing data were used to explore genetic scoring specific to FLG LOF mutations.ResultsGenetic scores derived from the AD-only genome-wide association studies were predictive of AD cases (PRSAD: odds ratio [OR], 1.70; 95% CI, 1.49-1.93). Accuracy was first improved when PRSs were built off the larger atopy genome-wide association studies (PRSAD+: OR, 2.16; 95% CI, 1.89-2.47) and further improved when including FLG LOF mutations (PRSAD++: OR, 3.23; 95% CI, 2.57-4.07). Importantly, while all 3 PRSs correlated with AD severity, the best prediction was from PRSAD++, which distinguished individuals with severe AD from control subjects with OR of 3.86 (95% CI, 2.77-5.36).ConclusionsThis study demonstrates how PRSs for AD that include genetic determinants across atopic phenotypes and FLG LOF variants may be a promising tool for identifying individuals at high risk for developing disease and specifically severe disease.

Published
2022

5. Multiethnic genome-wide and HLA association study of total serum IgE level

Author

Daya, Michelle, Cox, Corey, Acevedo, Nathalie, Boorgula, Meher P, Campbell, Monica, Chavan, Sameer, Cho, Michael H, David, Gloria L, Kachroo, Priyadarshini, Lasky-Su, Jessica, Li, Xingnan, McHugh, Caitlin P, Qiao, Dandi, Rafaels, Nicholas, Beck, Lisa A, Bleecker, Eugene R, Caraballo, Luis, Cupples, Adrienne L, Figueiredo, Camila A, Gallo, Richard L, Hanifin, Jon, Hansel, Nadia N, Hata, Tissa R, Hersh, Craig P, Knight-Madden, Jennifer, Leung, Donald YM, Guttman-Yassky, Emma, Meyers, Deborah A, O’Connor, George, Ober, Carole, Ong, Peck Y, Ortega, Victor E, Paller, Amy S, Putcha, Nirupama, Reed, Robert M, Schneider, Lynda C, Silverman, Edwin K, Slifka, Mark K, Spergel, Jonathan M, Vasan, Ramachandran S, Viaud-Martinez, Karine A, Watson, Harold, Weiss, Scott T, Consortium, NHLBI Trans-Omics for Precision Medicine, Ruczinski, Ingo, Beaty, Terri H, Mathias, Rasika A, and Barnes, Kathleen C

Subjects
Clinical Research, Genetics, Lung, Asthma, Human Genome, Aetiology, 2.1 Biological and endogenous factors, Inflammatory and immune system, Good Health and Well Being, Adolescent, Adult, Aged, Child, Child, Preschool, Dermatitis, Atopic, Ethnicity, Female, Gene Frequency, Genetic Predisposition to Disease, Genome-Wide Association Study, Genotype, HLA-A2 Antigen, HLA-DQ beta-Chains, Humans, Immunoglobulin E, Male, Middle Aged, National Heart, Lung, and Blood Institute (U.S.), United States, Whole Genome Sequencing, Young Adult, Total serum IgE, human leukocyte antigen, genome-wide association study, atopic dermatitis, asthma, multiethnic, NHLBI Trans-Omics for Precision Medicine (TOPMed) Consortium, Immunology, Allergy
Abstract

BackgroundTotal serum IgE (tIgE) is an important intermediate phenotype of allergic disease. Whole genome genetic association studies across ancestries may identify important determinants of IgE.ObjectiveWe aimed to increase understanding of genetic variants affecting tIgE production across the ancestry and allergic disease spectrum by leveraging data from the National Heart, Lung and Blood Institute Trans-Omics for Precision Medicine program; the Consortium on Asthma among African-ancestry Populations in the Americas (CAAPA); and the Atopic Dermatitis Research Network (N = 21,901).MethodsWe performed genome-wide association within strata of study, disease, and ancestry groups, and we combined results via a meta-regression approach that models heterogeneity attributable to ancestry. We also tested for association between HLA alleles called from whole genome sequence data and tIgE, assessing replication of associations in HLA alleles called from genotype array data.ResultsWe identified 6 loci at genome-wide significance (P < 5 × 10-9), including 4 loci previously reported as genome-wide significant for tIgE, as well as new regions in chr11q13.5 and chr15q22.2, which were also identified in prior genome-wide association studies of atopic dermatitis and asthma. In the HLA allele association study, HLA-A∗02:01 was associated with decreased tIgE level (Pdiscovery = 2 × 10-4; Preplication = 5 × 10-4; Pdiscovery+replication = 4 × 10-7), and HLA-DQB1∗03:02 was strongly associated with decreased tIgE level in Hispanic/Latino ancestry populations (PHispanic/Latino discovery+replication = 8 × 10-8).ConclusionWe performed the largest genome-wide association study and HLA association study of tIgE focused on ancestrally diverse populations and found several known tIgE and allergic disease loci that are relevant in non-European ancestry populations.

Published
2021

6. Growth faltering regardless of chronic diarrhea is associated with mucosal immune dysfunction and microbial dysbiosis in the gut lumen

Author

Rhoades, Nicholas S, Hendrickson, Sara M, Prongay, Kamm, Haertel, Andrew, Gill, Leanne, Edwards, Robert A, Garzel, Laura, Slifka, Mark K, and Messaoudi, Ilhem

Subjects
Pediatric, Clinical Research, Infectious Diseases, Foodborne Illness, Vaccine Related, Prevention, Digestive Diseases, Biodefense, Oral and gastrointestinal, Good Health and Well Being, Animals, Biodiversity, Biomarkers, Biopsy, Chronic Disease, Diarrhea, Disease Models, Animal, Disease Susceptibility, Dysbiosis, Gastrointestinal Microbiome, Growth Disorders, Immunity, Mucosal, Immunohistochemistry, Intestinal Mucosa, Lymphocyte Count, Macaca mulatta, Metagenome, Metagenomics, Transcriptome, Biological Sciences, Medical and Health Sciences, Immunology
Abstract

Despite the impact of childhood diarrhea on morbidity and mortality, our understanding of its sequelae has been significantly hampered by the lack of studies that examine samples across the entire intestinal tract. Infant rhesus macaques are naturally susceptible to human enteric pathogens and recapitulate the hallmarks of diarrheal disease such as intestinal inflammation and growth faltering. Here, we examined intestinal biopsies, lamina propria leukocytes, luminal contents, and fecal samples from healthy infants and those experiencing growth faltering with distant acute or chronic active diarrhea. We show that growth faltering in the presence or absence of active diarrhea is associated with a heightened systemic and mucosal pro-inflammatory state centered in the colon. Moreover, polyclonal stimulation of colonic lamina propria leukocytes resulted in a dampened cytokine response, indicative of immune exhaustion. We also detected a functional and taxonomic shift in the luminal microbiome across multiple gut sites including the migration of Streptococcus and Prevotella species between the small and large intestine, suggesting a decompartmentalization of gut microbial communities. Our studies provide valuable insight into the outcomes of diarrheal diseases and growth faltering not attainable in humans and lays the groundwork to test interventions in a controlled and reproducible setting.

Published
2021

7. State-of-the-Science of human papillomavirus vaccination in women with human immunodeficiency Virus: Summary of a scientific workshop

Author

Schuind, Anne E., Rees, Helen, Schiller, John, Mugo, Nelly, Dull, Peter, Barnabas, Ruanne, Clifford, Gary M., Liu, Gui, Madhi, Shabir A., Morse, Rebecca B., Moscicki, Anna-Barbara, Palefsky, Joel M., Plotkin, Stanley, Sierra, Mónica S., Slifka, Mark K., Vorsters, Alex, Kreimer, Aimée R., and Didierlaurent, Arnaud M.

Published
2023
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8. Loss of Preexisting Immunological Memory Among Human Immunodeficiency Virus–Infected Women Despite Immune Reconstitution With Antiretroviral Therapy

Author

Thomas, Archana, Hammarlund, Erika, Gao, Lina, Holman, Susan, Michel, Katherine G, Glesby, Marshall, Villacres, Maria C, Golub, Elizabeth T, Roan, Nadia R, French, Audrey L, Augenbraun, Michael H, and Slifka, Mark K

Subjects
Medical Microbiology, Biomedical and Clinical Sciences, Immunology, Emerging Infectious Diseases, Vaccine Related, Prevention, HIV/AIDS, Infectious Diseases, Sexually Transmitted Infections, Rare Diseases, Immunization, Clinical Research, Immunotherapy, Pediatric, Infection, Inflammatory and immune system, Good Health and Well Being, Adult, Anti-HIV Agents, Antibodies, Viral, CD3 Complex, CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Cohort Studies, Female, HIV Infections, Humans, Immune Reconstitution, Immunologic Memory, Lymphocyte Activation, Smallpox Vaccine, Time Factors, Vaccinia virus, HIV, ART, antiretroviral therapy, smallpox, vaccination, immunological memory, Biological Sciences, Medical and Health Sciences, Microbiology, Biological sciences, Biomedical and clinical sciences, Health sciences
Abstract

BackgroundIt is unclear whether human immunodeficiency virus (HIV) infection results in permanent loss of T-cell memory or if it affects preexisting antibodies to childhood vaccinations or infections.MethodsWe conducted a matched cohort study involving 50 pairs of HIV-infected and HIV-uninfected women. Total memory T-cell responses were measured after anti-CD3 or vaccinia virus (VV) stimulation to measure T cells elicited after childhood smallpox vaccination. VV-specific antibodies were measured by means of enzyme-linked immunosorbent assay (ELISA).ResultsThere was no difference between HIV-infected and HIV-uninfected study participants in terms of CD4+ T-cell responses after anti-CD3 stimulation (P = .19) although HIV-infected participants had significantly higher CD8+ T-cell responses (P = .03). In contrast, there was a significant loss in VV-specific CD4+ T-cell memory among HIV-infected participants (P = .04) whereas antiviral CD8+ T-cell memory remained intact (P > .99). VV-specific antibodies were maintained indefinitely among HIV-uninfected participants (half-life, infinity; 95% confidence interval, 309 years to infinity) but declined rapidly among HIV-infected participants (half-life; 39 years; 24-108 years; P = .001).ConclusionsDespite antiretroviral therapy-associated improvement in CD4+ T-cell counts (nadir, 350/μL after antiretroviral therapy), antigen-specific CD4+ T-cell memory to vaccinations or infections that occurred before HIV infection did not recover after immune reconstitution, and a previously unrealized decline in preexisting antibody responses was observed.

Published
2020

9. Contributors

Author

Abzug, Mark J., primary, Acharya, Priyamvada, additional, Acosta, Anna M., additional, Ahmed, S. Sohail, additional, Amanna, Ian J., additional, Anderson, Annaliesa S., additional, Asturias, Edwin J., additional, Bachmann, Martin F., additional, Bahl, Sunil, additional, Bailey, Justin R., additional, Baker, Carol J., additional, Balfour, Henry H., additional, Baric, Ralph S., additional, Barnett, Elizabeth D., additional, Barrett, Alan D.T., additional, Belser, Jessica A., additional, Berzofsky, Jay A., additional, Bethony, Jeffrey M., additional, Brewer-Jensen, Paul D., additional, Bubak, Andrew N., additional, Burns, Cara C., additional, Caplan, Arthur L., additional, Cavaleri, Marco, additional, Chandran, Aruna, additional, Cherian, Thomas, additional, Clemens, John D., additional, Cochino, Emil, additional, Cohet, Catherine, additional, Cohn, Amanda, additional, Cortese, Margaret M., additional, Crowcroft, Natasha S., additional, Curtis, Nigel, additional, Damron, F. Heath, additional, Danchin, Margie, additional, Debbink, Kari, additional, Desai, Sachin N., additional, Davis, Emily H., additional, Decker, Michael D., additional, Denison, Mark R., additional, DeStefano, Frank, additional, Douglas, R. Gordon, additional, Dreher-Lesnick, Sheila M., additional, Eberhardt, Christiane S., additional, Edmunds, W. John, additional, Edwards, Caitlin E., additional, Edwards, Kathryn M., additional, Eggers, Rudolf, additional, Ellis, Ronald, additional, Erdman, Dean D., additional, Ertl, Hildegund C.J., additional, Estivariz, Concepcion F., additional, Fine, Paul E.M., additional, Finn, Theresa M., additional, Fisher, Allison M., additional, Fitzwater, Sean Patrick, additional, Freedman, Mark S., additional, Friede, Martin, additional, Friedlander, Arthur M., additional, Frumento, Nicole, additional, Fry, Alicia M., additional, Garçon, Nathalie, additional, Geris, Jennifer M., additional, Gershon, Anne A., additional, Gervier, Regis, additional, Gessner, Bradford D., additional, Gilbert, Peter B., additional, Gomez, Phillip Louis, additional, Ginsberg, Ann M., additional, Grabenstein, John D., additional, Graham, Rachel L., additional, Graham, Barney S., additional, Granoff, Dan M., additional, Gray, Gregory C., additional, Greenberg, David P., additional, Grohskopf, Lisa A., additional, Gruber, Marion F., additional, Guerena, Fernando B., additional, Havelange, Nicolas, additional, Halstead, Scott B., additional, Hanekom, Willem A., additional, Harrison, Lee H., additional, Hawn, Thomas R., additional, Haynes, Barton F., additional, Healy, C. Mary, additional, Hills, Susan L., additional, Hirabayashi, Kuniko, additional, Holmgren, Jan, additional, Hombach, Joachim M., additional, Hotez, Peter Jay, additional, Howe, Barbara J., additional, Hunegnaw, Ruth, additional, Izopet, Jacques, additional, Jamieson, Denise J., additional, Jansen, Kathrin, additional, Jarrahian, Courtney, additional, Johansen, Kari, additional, Kahn, Geoffrey D., additional, Karron, Ruth A., additional, Katz, Jacqueline M., additional, Kennedy, Richard B., additional, Broojerdi, Alireza Khadem, additional, Khetsuriani, Nino, additional, Khudyakov, Yury, additional, Klugman, Keith P., additional, Kocher, Jacob F., additional, Kollaritsch, Herwig, additional, Kotloff, Karen L., additional, Kozarsky, Phyllis E., additional, Kreimer, Aimée R., additional, Kroger, Andrew T., additional, Kwong, Peter D., additional, Lal, Manjari, additional, Levin, Myron J., additional, Levine, Myron M., additional, Lindesmith, Lisa C., additional, Lindstrand, Ann, additional, Ljungman, Per, additional, Lowy, Douglas R., additional, Lundgren, Anna, additional, Lydon, Patrick, additional, Macklin, Grace R., additional, Maeng, Hoyoung M., additional, Mahalingam, Ravi, additional, Malley, Richard, additional, Mallory, Michael L., additional, Marfin, Anthony A., additional, Markowitz, Lauri E., additional, Marshall, CDR Valerie, additional, McCollum, Andrea, additional, Meyer, Sarah, additional, McNamara, Lucy A., additional, Menning, Lisa, additional, Messacar, Kevin, additional, Miller, Mark A., additional, Milutinovic, Pavle, additional, Modlin, John F., additional, Monath, Thomas P., additional, Morabito, Kaitlyn M., additional, Moss, William J., additional, Mulholland, Kim, additional, Mura, Manuela, additional, Musher, Daniel M., additional, Nagel, Maria A., additional, Nair, G. Balakrish, additional, Nelson, Noele P., additional, Netea, Mihai G., additional, Neuzil, Kathleen Maletic, additional, Niemeyer, Christy S., additional, Nohynek, Hanna, additional, Norheim, Gunnstein, additional, Nussbaum, Lauren, additional, O’Brien, Katherine L., additional, O’Leary, Sean, additional, Ockenhouse, Christian, additional, Offit, Paul A., additional, Okwo-Bele, Jean-Marie, additional, Olkhanud, Purevdorj B., additional, Omer, Saad B., additional, Orenstein, Walter A., additional, Oyston, Petra C.F., additional, Parashar, Umesh D., additional, Patel, Manish M., additional, Payne, Daniel C., additional, Pebody, Richard, additional, Pecetta, Simone, additional, Perlman, Stanley, additional, Pierson, Benjamin, additional, Pierson, Theodore C., additional, Pittet, Laure F., additional, Pittman, Phillip R., additional, Plotkin, Stanley A., additional, Plotkin, Susan L., additional, Poland, Gregory A., additional, Pollard, Sir Andrew John, additional, Poovorawan, Yong, additional, Proctor, Richard A., additional, Qadri, Firdausi, additional, Rao, Agam, additional, Rappuoli, Rino, additional, Reef, Susan E., additional, Rogalewicz, Joseph A., additional, Robinson, James Michael, additional, Roesel, Sigrun, additional, Rubin, Steven A., additional, Rupprecht, Charles E., additional, Rutter, Paul, additional, Samant, Vijay B., additional, Sambhara, Suryaprakash, additional, Samies, Nicole L., additional, Santosham, Mathuram, additional, Saunders, Kevin O., additional, Schiller, John T., additional, Schleiss, Mark R., additional, Schuerman, Lode, additional, Schwab, Jennifer, additional, Schwartz, Jason L., additional, Scobie, Heather M., additional, Scott, J. Anthony, additional, Shapiro, Eugene D., additional, Shenoy, Erica S., additional, Shimabukuro, Tom T., additional, Shouval, Daniel, additional, Siegrist, Claire-Anne, additional, Sitrin, Robert D., additional, Skinner, Nicole E., additional, Slifka, Mark K., additional, Sodha, Samir V., additional, Soeters, Heidi M., additional, Solomon, Tom, additional, Speiser, Daniel E., additional, Staples, J. Erin, additional, Steffen, Robert, additional, Stephens, David S., additional, Strebel, Peter M., additional, Subbarao, Kanta, additional, Sullivan, Nancy J., additional, Takashima, Yoshihiro, additional, Tate, Jacqueline E., additional, Tayman, Alice, additional, Telford, Sam R., additional, Thombley, Melisa, additional, Thomsen, Isaac, additional, Tohme, Rania A., additional, Trabold, Malin, additional, El-Turabi, Aadil, additional, Vaughn, David W., additional, Verardi, Raffaello, additional, Vicari, Andrea S., additional, Vidor, Emmanuel J., additional, Villafana, Tonya, additional, Vogt, Matthew R., additional, Walker, Mark J., additional, Walsh, Nick M., additional, Wanlapakorn, Nasamon, additional, Ward, John W., additional, Wassilak, Steven G.F., additional, Watts, Lisa A., additional, Weber, David J., additional, Weiner, David B., additional, Weng, Mark K., additional, Wexler, Deborah L., additional, Wharton, Melinda, additional, Whitley, Richard J., additional, Whitney, Cynthia G., additional, Wiehe, Kevin, additional, Williamson, E. Diane, additional, Wormser, Gary P., additional, Xia, Ningshao, additional, Yildirim, Inci, additional, Zehrung, Darin, additional, and Zweigart, Mark R., additional

Published
2023
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12. Successful Vaccines

Author

Amanna, Ian J., Slifka, Mark K., Ahmed, Rafi, Series Editor, Akira, Shizuo, Series Editor, Aktories, Klaus, Series Editor, Casadevall, Arturo, Series Editor, Compans, Richard W, Series Editor, Galan, Jorge E, Series Editor, Garcia-Sastre, Adolfo, Series Editor, Malissen, Bernard, Series Editor, Rappuoli, Rino, Series Editor, Hangartner, Lars, editor, and Burton, Dennis R., editor

Published
2020
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13. T-bet+ B cells are induced by human viral infections and dominate the HIV gp140 response

Author

Knox, James J, Buggert, Marcus, Kardava, Lela, Seaton, Kelly E, Eller, Michael A, Canaday, David H, Robb, Merlin L, Ostrowski, Mario A, Deeks, Steven G, Slifka, Mark K, Tomaras, Georgia D, Moir, Susan, Moody, M Anthony, and Betts, Michael R

Subjects
Medical Microbiology, Biomedical and Clinical Sciences, Immunology, Prevention, HIV/AIDS, Infectious Diseases, Immunization, Emerging Infectious Diseases, Sexually Transmitted Infections, Vaccine Related (AIDS), Vaccine Related, 2.1 Biological and endogenous factors, 2.2 Factors relating to the physical environment, Aetiology, Infection, Good Health and Well Being, AIDS/HIV, Biomedical and clinical sciences, Health sciences
Abstract

Humoral immunity is critical for viral control, but the identity and mechanisms regulating human antiviral B cells are unclear. Here, we characterized human B cells expressing T-bet and analyzed their dynamics during viral infections. T-bet+ B cells demonstrated an activated phenotype, a distinct transcriptional profile, and were enriched for expression of the antiviral immunoglobulin isotypes IgG1 and IgG3. T-bet+ B cells expanded following yellow fever virus and vaccinia virus vaccinations and also during early acute HIV infection. Viremic HIV-infected individuals maintained a large T-bet+ B cell population during chronic infection that was associated with increased serum and cell-associated IgG1 and IgG3 expression. The HIV gp140-specific B cell response was dominated by T-bet-expressing memory B cells, and we observed a concomitant biasing of gp140-specific serum immunoglobulin to the IgG1 isotype. These findings suggest that T-bet induction promotes antiviral immunoglobulin isotype switching and development of a distinct T-bet+ B cell subset that is maintained by viremia and coordinates the HIV Env-specific humoral response.

Published
2017

18. Development of a novel serology assay for the detection of IgG antibodies to identify exposures to orthopoxviruses

Author

Lee, William Thomas, primary, Hunt, Danielle T, additional, Kulas, Karen E, additional, Howard, Kelly M, additional, Carson, Kyle, additional, Lamson, Theresa, additional, Chakravarthi, Sandhya, additional, Chave, Karen, additional, Bievenue, Rachel, additional, Rock, Jean M, additional, Styer, Linda M, additional, Noone, Philip, additional, Hogrefe, Wayne, additional, Cai, Gianna Y, additional, Bermúdez-González, Maria C, additional, Kleiner, Giulio, additional, Srivastava, Komal, additional, Simon, Viviana, additional, Feihel, Dennis, additional, McGowan, Joseph P, additional, Slifka, Mark K, additional, and Yates, Jennifer L, additional

Published
2023
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19. Development of a Novel Serological Assay for the Detection of Mpox Infection in Vaccinated Populations

Author

Yates, Jennifer L, primary, Hunt, Danielle T., additional, Kulas, Karen E., additional, Chave, Karen, additional, Styer, Linda, additional, Chakravarthi, Sandhya T., additional, Cai, Gianna Y., additional, Bermudez-Gonzalez, Maria C., additional, Kleiner, Giulio, additional, Altman, Deena R L, additional, Srivastava, Komal, additional, Study Group, PVI, additional, Simon, Viviana, additional, Feihel, Dennis, additional, McGowan, Joseph, additional, Hogrefe, Wayne, additional, Noone, Philip, additional, Egan, Christina, additional, Slifka, Mark K., additional, and Lee, William, additional

Published
2023
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20. Therapeutic neutralizing monoclonal antibody administration protects against lethal yellow fever virus infection

Author

Ricciardi, Michael J., primary, Rust, Lauren N., additional, Pedreño-Lopez, Nuria, additional, Yusova, Sofiya, additional, Biswas, Sreya, additional, Webb, Gabriela M., additional, Gonzalez-Nieto, Lucas, additional, Voigt, Thomas B., additional, Louw, Johan J., additional, Laurino, Fernanda D., additional, DiBello, John R., additional, Raué, Hans-Peter, additional, Barber-Axthelm, Aaron M., additional, Chun, Kimberly, additional, Uttke, Samantha, additional, Raphael, Lidiane M. S., additional, Yrizarry-Medina, Aaron, additional, Rosen, Brandon C., additional, Agnor, Rebecca, additional, Gao, Lina, additional, Labriola, Caralyn, additional, Axthelm, Michael, additional, Smedley, Jeremy, additional, Julander, Justin G., additional, Bonaldo, Myrna C., additional, Walker, Laura M., additional, Messaoudi, Ilhem, additional, Slifka, Mark K., additional, Burton, Dennis R., additional, Kallas, Esper G., additional, Sacha, Jonah B., additional, Watkins, David I., additional, and Burwitz, Benjamin J., additional

Published
2023
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22. Development of a Novel Serological Assay for the Detection of Mpox Infection in Vaccinated Populations

Author

Yates, Jennifer L., Hunt, Danielle T., Kulas, Karen E., Chave, Karen, Styer, Linda, Chakravarthi, Sandhya T., Cai, Gianna Y., Bermúdez-González, Maria C., Kleiner, Giulio, Altman, Deena, Srivastava, Komal, Simon, Viviana, Feihel, Dennis, McGowan, Joseph, Hogrefe, Wayne, Noone, Philip, Egan, Christina, Slifka, Mark K., and Lee, William T.

Subjects
Article
Abstract

In 2022 the World Health Organization declared a Public Health Emergency for an outbreak of mpox, the zoonotic Orthopoxvirus (OPV) affecting at least 103 non-endemic locations world-wide. Serologic detection of mpox infection is problematic, however, due to considerable antigenic and serologic cross-reactivity among OPVs and smallpox-vaccinated individuals. In this report, we developed a high-throughput multiplex microsphere immunoassay (MIA) using a combination of mpox-specific peptides and cross-reactive OPV proteins that results in the specific serologic detection of mpox infection with 93% sensitivity and 98% specificity. The New York State Non-Vaccinia Orthopoxvirus Microsphere Immunoassay is an important diagnostic tool to detect subclinical mpox infection and understand the extent of mpox spread in the community through retrospective analysis.

Published
2023

23. Contributors

Author

Abrignani, Sergio, primary, Ahmed, S. Sohail, additional, Amanna, Ian J., additional, Anderson, Teresa A., additional, Arlett, Peter R., additional, Atkinson, William L., additional, Averhoff, Francisco M., additional, Aylward, R. Bruce, additional, Bachmann, Martin F., additional, Baker, Carol J., additional, Balfour, Henry H., additional, Ballou, W. Ripley, additional, Baric, Ralph S., additional, Barrett, Alan D.T., additional, Barnett, Elizabeth D., additional, Belgharbi, Lahouari, additional, Berinstein, Elliot M., additional, Berinstein, Neil L., additional, Bethony, Jeffrey M., additional, Bogaerts, Hugues, additional, Bot, Adrian, additional, Brachman, Philip S., additional, Bresee, Joseph S., additional, Broojerdi, Alireza Khadem, additional, Caplan, Arthur L., additional, Cavaleri, Marco, additional, Cherian, Thomas, additional, Choi-Sing, Pele, additional, Clemens, John D., additional, Cochi, Stephen L., additional, Cohn, Amanda, additional, Cortese, Capt. Margaret M., additional, Cox, Nancy J., additional, Cutts, Felicity, additional, Dagan, Ron, additional, Dalton, Harry R., additional, Daum, Robert S., additional, Davey, Andrea Sudell, additional, De Francesco, Raffaele, additional, Debbink, Kari, additional, Decker, Michael D., additional, Desai, Sachin N., additional, DeStefano, Frank, additional, Douglas, R. Gordon, additional, Dubischar, Katrin, additional, Edmunds, W. John, additional, Edwards, Kathryn M., additional, Egan, William, additional, Eggers, Rudolf, additional, Ehmann, Falk, additional, Ellis, Ronald W., additional, El-Turabi, Aadil, additional, Erdman, Dean D., additional, Ertl, Hildegund, additional, Fine, Paul E.M., additional, Finn, Theresa M., additional, Fisher, Allison, additional, Friede, Martin, additional, Friedlander, Arthur M., additional, Fry, Alicia M., additional, Garçon, Nathalie, additional, Gastañaduy, Paul A., additional, Gershman, Mark D., additional, Gershon, Anne A., additional, Gessner, Bradford D., additional, Gilbert, Peter, additional, Ginsberg, Ann M., additional, Girard, Marc P., additional, Gomez, Phillip L., additional, Goodson, James L., additional, Goodwin, Robert R., additional, Gordon, Lance K., additional, Grabenstein, John D., additional, Graham, Barney S., additional, Graham, Rachel L., additional, Granoff, Dan M., additional, Gray, Gregory C., additional, Gruber, Marion F., additional, Halstead, Scott B., additional, Hanekom, Willem, additional, Harrison, Lee H., additional, Hawn, Thomas R., additional, Healy, C. Mary, additional, Henderson, Donald A., additional, Hildesheim, Allan, additional, Hills, Susan L., additional, Holmgren, Jan, additional, Hombach, Joachim, additional, Hotez, Peter J., additional, Houghton, Michael, additional, Houston, Avril Melissa, additional, Howe, Barbara J., additional, Izopet, Jacques, additional, Jamieson, Denise J., additional, Jarrahian, Courtney, additional, Johansen, Kari, additional, Karron, Ruth A., additional, Kennedy, Richard B., additional, Kew, Olen M., additional, Khudyakov, Yury, additional, Klein, Michel, additional, Klugman, Keith P., additional, Kocher, Jacob F., additional, Koff, Wayne C., additional, Kollaritsch, Herwig, additional, Kotloff, Karen L., additional, Kozarsky, Phyllis E., additional, Kroger, Andrew T., additional, Kurz, Xavier, additional, Lakdawala, Seema S., additional, Lane, J. Michael, additional, Leigh, Kendra, additional, Levin, Myron J., additional, Levine, Emily Marcus, additional, Levine, Myron M., additional, Lindesmith, Lisa C., additional, Ljungman, Per, additional, Lowy, Douglas R., additional, Luke, Catherine J., additional, Lundgren, Anna, additional, Lydon, Patrick, additional, Malley, Richard, additional, Marin, Mona, additional, Markowitz, Lauri E., additional, Marshall, Lieut. Valerie B., additional, Miller, Mark A., additional, Monath, Thomas P., additional, Moss, William J., additional, Mulholland, Kim, additional, Musher, Daniel M., additional, Nabel, Gary J., additional, Nagarajan, Thirumeni, additional, Nair, GB, additional, Nanduri, Srinivas Acharya, additional, Neddermann, Petra, additional, Nelson, Noele P., additional, Offit, Paul A., additional, Okwo-Bele, Jean-Marie, additional, Omer, Saad B., additional, Orenstein, Walter A., additional, Oyston, Petra C.F., additional, Papania, Mark J., additional, Parashar, Umesh D., additional, Pfeifer, Dina, additional, Pickering, Larry K., additional, Pittman, Phillip R., additional, Ploquin, Aurélie, additional, Plotkin, Stanley A., additional, Plotkin, Susan L., additional, Poland, Gregory A., additional, Pollard, Andrew J., additional, Qadri, Firdausi, additional, Quirk, Mary R., additional, Rao, Raman D.S.V., additional, Rappuoli, Rino, additional, Reef, Susan E., additional, Ridpath, Alison D., additional, Robinson, James M., additional, Rodewald, Lance E., additional, Rodriguez-Hernandez, Carmen A., additional, Roper, Martha H., additional, Rubin, Steven A., additional, Rupprecht, Charles E., additional, Rutala, William A., additional, Salisbury, David, additional, Samant, Vijay B., additional, Sambhara, Suryaprakash, additional, Santosham, Mathuram, additional, Schiller, John T., additional, Schleiss, Mark R., additional, Schuchat, Anne, additional, Schwartz, Jason L., additional, Scobie, Heather M., additional, Scott, J. Anthony, additional, Seward, Jane F., additional, Shouval, Daniel, additional, Siegrist, Claire-Anne, additional, Slifka, Mark K., additional, Sodha, Samir V., additional, Stanberry, Lawrence R., additional, Staples, J. Erin, additional, Steere, Allen C., additional, Steffen, Robert, additional, Strebel, Peter M., additional, Subbarao, Kanta, additional, Sullivan, Nancy J., additional, Sutcliffe, Catherine G., additional, Sutherland, Andrea R., additional, Sutter, Roland W., additional, Thomas, Stephen J., additional, Tiwari, Tejpratap S.P, additional, Tsai, Theodore F., additional, Van Damme, Pierre, additional, Vekemans, Johan, additional, Vidor, Emmanuel, additional, Ward, John W., additional, Wassilak, Steven G.F., additional, Weber, David J., additional, Weiner, David B., additional, Wexler, Deborah L., additional, Wharton, Melinda, additional, Whitney, Cynthia G., additional, Williamson, E. Diane, additional, Wood, David J., additional, Xia, Ningshao, additional, Xu, Zhi Yi, additional, Zanetti, Alessandro, additional, and Zehrung, Darin, additional

Published
2018
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27. Signaling sphingolipids are biomarkers for atopic dermatitis prone to disseminated viral infections

Author

Berdyshev, Evgeny, primary, Goleva, Elena, additional, Bronova, Irina, additional, Bronoff, Anna Sofia, additional, Streib, Joanne E., additional, Vang, Kathryn A., additional, Richers, Brittany N., additional, Taylor, Patricia, additional, Beck, Lisa, additional, Villarreal, Miguel, additional, Johnson, Keli, additional, David, Gloria, additional, Slifka, Mark K., additional, Hanifin, Jon, additional, and Leung, Donald Y.M., additional

Published
2022
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36. Therapeutic neutralizing monoclonal antibody administration protects against lethal viscerotropic Yellow Fever infection

Author

Ricciardi, Michael J., primary, Rust, Lauren N., additional, Pedreño-Lopez, Nuria, additional, Yusova, Sofiya, additional, Biswas, Sreya, additional, Webb, Gabriela M., additional, Fischer, Miranda, additional, Gonsales-Nieto, Lucas, additional, Voigt, Thomas B., additional, Louw, Johan J., additional, Laurino, Fernanda D., additional, DiBello, John R., additional, Raué, Hans-Peter, additional, Raphael, Lidiane M.S., additional, Yrizarry-Medina, Aaron, additional, Rosen, Brandon C., additional, Agnor, Rebecca, additional, Gao, Lina, additional, Labriola, Caralyn, additional, Axthelm, Michael, additional, Smedley, Jeremy, additional, Julander, Justin G., additional, Bonaldo, Myrna C., additional, Walker, Laura M., additional, Slifka, Mark K., additional, Messaoudi, Ilhem, additional, Burton, Dennis R., additional, Kallas, Esper G., additional, Sacha, Jonah B., additional, Watkins, David I., additional, and Burwitz, Benjamin J., additional

Published
2022
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37. Immunogenicity of Pfizer mRNA COVID-19 Vaccination Followed by J&J Adenovirus COVID-19 Vaccination in Two Patients with Chronic Lymphocytic Leukemia

Author

Lyski, Zoe L., Kim, Myung Sun, Xthona Lee, David, Sampson, David, Raué, Hans-Peter, Raghunathan, Vikram, Ryan, Debbie, Brunton, Amanda E., Slifka, Mark K., Messer, William B., and Spurgeon, Stephen E.

Subjects
Article Subject
Abstract

Individuals with chronic lymphocytic leukemia (CLL) have significant immune disfunction, often further disrupted by treatment. While currently available COVID-19 vaccinations are highly effective in immunocompetent individuals, they are often poorly immunogenic in CLL patients. It is important to understand the role a heterologous boost would have in patients who did not respond to the initial two-dose mRNA vaccine series. SARS-CoV-2 specific immune responses, including antibodies and memory B-cells, CD4 and CD8 T-cells were assessed prior to vaccination, as well as postinitial vaccination series and post-third dose in two subjects. One subject seroconverted, had RBD-specific memory B-cells and spike-specific CD4 T-cells while the other did not. Both subjects had a spike-specific CD8 T-cell response after the original mRNA vaccination series that was further boosted after the third dose or remained stable. The results of this study, however small, are especially promising to CLL individuals who did not seroconvert following the initial mRNA vaccination series.

Published
2022
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40. Analysis of COVID-19 Transmission: Low Risk of Presymptomatic Spread?

Author

Slifka, Mark K., Messer, William B., and Amanna, Ian J.

Subjects
Disease transmission -- Observations, Epidemics -- Control -- United States, Medical care quality -- Evaluation, COVID-19 -- Control, Health
Abstract

To the Editor.--More than 6 million confirmed cases of coronavirus disease 2019 (COVID-19) have been identified worldwide, and a number of case reports (1-5) have indicated that COVID-19 has the [...]

Published
2020
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