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This paper aims to reveal the annual regime, time series, and long-term water temperature trends of the Danube River at Bratislava, Slovakia, between the years 1926 and 2005. First, the main factors affecting the river’s water temperature were identified. Using multiple regression techniques, an empirical relationship is derived between monthly water temperatures and monthly atmospheric temperatures at Vienna (Hohe Warte), Austria, monthly discharge of the Danube, and some other factors as well. In the second part of the study, the long-term trends in the annual time series of water temperature were identified. The following series were evaluated: 1) The average annual water temperature (To) (determined as an arithmetic average of daily temperatures in the Danube at Bratislava), 2) the weighted annual average temperature values (Toυ) (determined from the daily temperatures weighted by the daily discharge rates at Bratislava), and 3) the average heat load (Zt) at the Bratislava station. In the long run, the To series is rising; however, the trend of the weighted long-term average temperature values, Toυ, is near zero. This result indicates that the average heat load of the Danube water did not change during the selected period of 80 yr. What did change is the interannual distribution of the average monthly discharge. Over the past 25 yr, an elevated runoff of “cold” water (increase of the December–April runoff) and a lower runoff of “warm” water (decrease of the river runoff during the summer months of June–August) were observed. [ABSTRACT FROM AUTHOR]