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Your search keyword '"Skinner, John M."' showing total 40 results
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40 results on '"Skinner, John M."'

1. AMX – the highly automated macromolecular crystallography (17-ID-1) beamline at the NSLS-II

Author

Schneider, Dieter K., primary, Soares, Alexei S., additional, Lazo, Edwin O., additional, Kreitler, Dale F., additional, Qian, Kun, additional, Fuchs, Martin R., additional, Bhogadi, Dileep K., additional, Antonelli, Steve, additional, Myers, Stuart S., additional, Martins, Bruno S., additional, Skinner, John M., additional, Aishima, Jun, additional, Bernstein, Herbert J., additional, Langdon, Thomas, additional, Lara, John, additional, Petkus, Robert, additional, Cowan, Matt, additional, Flaks, Leonid, additional, Smith, Thomas, additional, Shea-McCarthy, Grace, additional, Idir, Mourad, additional, Huang, Lei, additional, Chubar, Oleg, additional, Sweet, Robert M., additional, Berman, Lonny E., additional, McSweeney, Sean, additional, and Jakoncic, Jean, additional

Published
2022
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3. FMX – the Frontier Microfocusing Macromolecular Crystallography Beamline at the National Synchrotron Light Source II

Author

Schneider, Dieter K., primary, Shi, Wuxian, additional, Andi, Babak, additional, Jakoncic, Jean, additional, Gao, Yuan, additional, Bhogadi, Dileep K., additional, Myers, Stuart F., additional, Martins, Bruno, additional, Skinner, John M., additional, Aishima, Jun, additional, Qian, Kun, additional, Bernstein, Herbert J., additional, Lazo, Edwin O., additional, Langdon, Thomas, additional, Lara, John, additional, Shea-McCarthy, Grace, additional, Idir, Mourad, additional, Huang, Lei, additional, Chubar, Oleg, additional, Sweet, Robert M., additional, Berman, Lonny E., additional, McSweeney, Sean, additional, and Fuchs, Martin R., additional

Published
2021
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6. Synchrotron X-ray-induced photoreduction of ferric myoglobin nitrite crystals gives the ferrous derivative with retention of the O-bonded nitrite ligand

Author

Jun Yi, Orville, Allen M., Skinner, John M., Skinner, Michael J., and Richter-Addo, George B.

Subjects
Crystallography -- Usage, Iron -- Chemical properties, Iron -- Atomic properties, Myoglobin -- Structure, Myoglobin -- Chemical properties, Myoglobin -- Optical properties, Nitrites -- Chemical properties, Synchrotron radiation -- Usage, Biological sciences, Chemistry
Abstract

Correlated single-crystal spectroscopy with crystallography was employed to characterize the single crystal of the nitrite adduct of ferric myoglobin (Mb) exposed to high-intensity synchrotron X-ray radiation. The 1.55 [Angstrom] resolution crystal structure of the photoproduct reveals retention of the O-binding mode for binding of nitrite to the iron center.

Published
2010

9. Solvent minimization induces preferential orientation and crystal clustering in serial micro-crystallography on micro-meshes,in situplates and on a movable crystal conveyor belt

Author

Soares, Alexei S., primary, Mullen, Jeffrey D., additional, Parekh, Ruchi M., additional, McCarthy, Grace S., additional, Roessler, Christian G., additional, Jackimowicz, Rick, additional, Skinner, John M., additional, Orville, Allen M., additional, Allaire, Marc, additional, and Sweet, Robert M., additional

Published
2014
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10. Macromolecular crystallography beamline X25 at the NSLS

Author

Héroux, Annie, primary, Allaire, Marc, additional, Buono, Richard, additional, Cowan, Matthew L., additional, Dvorak, Joseph, additional, Flaks, Leon, additional, LaMarra, Steven, additional, Myers, Stuart F., additional, Orville, Allen M., additional, Robinson, Howard H., additional, Roessler, Christian G., additional, Schneider, Dieter K., additional, Shea-McCarthy, Grace, additional, Skinner, John M., additional, Skinner, Michael, additional, Soares, Alexei S., additional, Sweet, Robert M., additional, and Berman, Lonny E., additional

Published
2014
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21. Solvent minimization induces preferential orientation and crystal clustering in serial micro-crystallography on micro-meshes, in situ plates and on a movable crystal conveyor belt.

Author

Soares, Alexei S., Mullen, Jeffrey D., Parekh, Ruchi M., McCarthy, Grace S., Roessler, Christian G., Jackimowicz, Rick, Skinner, John M., Orville, Allen M., Allaire, Marc, and Sweet, Robert M.

Subjects
X-ray diffraction, SYNCHROTRONS, LIGHT sources, LYSOZYMES, SINGLE crystals, CONVEYOR belts
Abstract

X-ray diffraction data were obtained at the National Synchrotron Light Source from insulin and lysozyme crystals that were densely deposited on three types of surfaces suitable for serial micro-crystallography: MiTeGen MicroMeshes2, Greiner Bio-One Ltd in situ micro-plates, and a moving kapton crystal conveyor belt that is used to deliver crystals directly into the X-ray beam. 6 wedges of data were taken from 100 crystals mounted on each material, and these individual data sets were merged to form nine complete data sets (six from insulin crystals and three from lysozyme crystals). Insulin crystals have a parallelepiped habit with an extended flat face that preferentially aligned with the mounting surfaces, impacting the data collection strategy and the design of the serial crystallography apparatus. Lysozyme crystals had a cuboidal habit and showed no preferential orientation. Preferential orientation occluded regions of reciprocal space when the X-ray beam was incident normal to the datacollection medium surface, requiring a second pass of data collection with the apparatus inclined away from the orthogonal. In addition, crystals measuring less than 20 mm were observed to clump together into clusters of crystals. Clustering required that the X-ray beam be adjusted to match the crystal size to prevent overlapping diffraction patterns. No additional problems were encountered with the serial crystallography strategy of combining small randomly oriented wedges of data from a large number of specimens. Highquality data able to support a realistic molecular replacement solution were readily obtained from both crystal types using all three serial crystallography strategies. [ABSTRACT FROM AUTHOR]

Published
2014
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24. Autism and Law Enforcement

Author

Skinner, John M.

Subjects
Autism and Law Enforcement (Video recording) -- Debbaudt, Dennis -- Legacy, Dave, Video recordings -- Video recording reviews, Law
Abstract

Autism and Law Enforcement, produced by Dennis Debbaudt and directed by Dave Legacy, April 2004. Chief executives in the 21st century face incredible challenges meeting training needs of their respective [...]

Published
2005

28. Single-crystal Raman spectroscopy and X-ray crystallography at beamline X26-C of the NSLS.

Author

Stoner-Ma, Deborah, Skinner, John M., Schneider, Dieter K., Cowan, Matt, Sweet, Robert M., and Orville, Allen M.

Subjects
*X-ray crystallography, *RAMAN effect, *RAMAN spectroscopy, *X-ray diffraction, *SYSTEM integration
Abstract

The article presents a study on the use of x-ray crystallography and single-crystal Raman spectroscopy in beamline X26-C of the National Synchrotron Light Source (NSLS). It states that Raman spectroscopy and x-ray diffraction were applied to set beamline X26-C by aligning the focal points of a camera and integrating software to collect data and Raman spectra. Furthermore, findings show that the combined use of x-ray and Raman resulted to specific vibrational mode shifting of crystals.

Published
2011
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30. Integrated software for macromolecular crystallography synchrotron beamlines II: revision, robots and a database.

Author

Cowan, Matt, Skinner, John M., Buono, Rick, Nolan, William, Bosshard, Heinz, Robinson, Howard H., Soares, Alexei S., Schneider, Dieter K., Héroux, Annie, and Sweet, Robert M.

Subjects
*INTEGRATED software, *CRYSTALLOGRAPHY, *X-ray diffractometers, *ROBOT control systems, *SYNCHROTRONS, *APPLICATION software, *INFORMATION storage & retrieval systems, *AUTOMATION
Abstract

This manuscript chronicles the evolution of software used originally to control a diffractometer at a macromolecular crystallography beamline. The system has been augmented and rewritten. A modular and carefully organized suite of programs now handles the whole experimental environment from a single vantage point. It provides automatic logging of the experiment and communication with the user, all the way from an initial proposal to perform the work to the end of data collection. This has included construction of a relational database to organize all details of the experiment and incorporation of a robotic specimen changer to provide automation for high-throughput applications. [ABSTRACT FROM AUTHOR]

Published
2006
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31. Production of a polyspecific human monoclonal antibody reacting with an epidermal antigen.

Author

Hohmann, Arthur W., Co1eman, Mark, Comacchio, Raffaela M., Skinner, John M., and Bradley, John

Subjects
CLONE cells, ANTIGENS, SKIN tumors, MONOCLONAL antibodies, IMMUNOGLOBULIN M, AUTOANTIBODIES
Abstract

A clone of cells secreting an antibody to an epidermal antigen was generated from a patient with a blistering skin lesion. Although produced by fusion of human lymphocytes to a HAT-sensitive myeloma. this clone of cells did not have characteristics of a hybridoma. A true hybridoma was produced by fusion of this clone to a HAT rouabainr myeloma line. The IgM antibody secreted by this clone reacted with the intercellular region of the epidermis of normal human skin in a manner similar to pemphigus autoantibodies. In addition, in normal human kidney the antibody hound to glomeruli and tubules. It also reacted with an antigen present in the cytoplasm of a wide variety of cell lines including epithelial, lymphoid and myeloid types. No reaction was found with the surface of any of the cell lines. nor with DNA or phospholipid antigens. This monoclonal antibody may define an autoantibody specificity which mediates some autoimmune skin lesions. its polyspecificity is reminiscent of some other human hybridoma autoantibodies. and its reaction with components of the kidney suggests an alternative pathology for renal disease in such patients. [ABSTRACT FROM AUTHOR]

Published
1988
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