307 results on '"Siyi Liu"'
Search Results
2. Employee perceived overqualification and innovation performance: the roles of self-oriented perfectionism and job crafting
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Bing Jiang, Hongxin Qiu, Siyi Liu, and Ji Zhang
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perceived overqualification ,self-oriented perfectionism ,job crafting ,innovation performance ,independent self-construction ,informal status ,Psychology ,BF1-990 - Abstract
Leveraging the trait activation theory, the study constructs a model featuring moderated chain mediation to explore how perceived overqualification influences employee innovation performance. After conducting two surveys with Chinese employees, this study collects 363 valid questionnaires. The findings reveal that perceived overqualification is positively related to employee innovation performance. Both self-oriented perfectionism and job crafting are partial mediators between perceived overqualification and innovation performance, and they collectively play a chain mediating role. Furthermore, independent self-construction positively moderates the link between perceived overqualification and self-oriented perfectionism, and informal status positively moderates the relationship between job crafting and employee innovation performance. Additionally, the indirect influence of perceived overqualification on employee innovation performance is moderated by independent self-construction and informal status. This study adds to the current body of literature on perceived overqualification and offers practical implications for organizations aiming to enhance innovation performance.
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- 2024
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3. Global changes of miRNA expression indicates an increased reprogramming efficiency of induced mammary epithelial cells by repression of miR-222-3p in fibroblasts
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Mingxing Liu, Siyi Liu, Liangshan Qin, Danwei Lv, Guodong Wang, Quanhui Liu, Ben Huang, and Dandan Zhang
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MicroRNA ,Reprogramming ,Induced mammary epithelial cells ,Transcriptional regulation ,Epigenetic reprogramming ,Medicine ,Biology (General) ,QH301-705.5 - Abstract
Background Our previous studies have successfully reported the reprogramming of fibroblasts into induced mammary epithelial cells (iMECs). However, the regulatory relationships and functional roles of MicroRNAs (miRNAs) in the progression of fibroblasts achieving the cell fate of iMECs are insufficiently understood. Methods First, we performed pre-and post-induction miRNAs sequencing analysis by using high-throughput sequencing. Following that, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment studies were used to determine the primary roles of the significantly distinct miRNAs and targeted genes. Finally, the effect of miR-222-3p on iMECs fate reprogramming in vitro by transfecting. Results As a result goat ear fibroblasts (GEFs) reprogramming into iMECs activates a regulatory program, involving 79 differentially expressed miRNAs. Besides, the programming process involved changes in multiple signaling pathways such as adherens junction, TGF-β signaling pathway, GnRH secretion and the prolactin signaling pathway, etc. Furthermore, it was discovered that the expression of miR-222-3p downregulation by miR-222-3p inhibitor significantly increase the reprogramming efficiency and promoted lipid accumulation of iMECs.
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- 2024
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4. The regulatory relationship between transcription factor STAT3 and noncoding RNA
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Siyi Liu, Wentao Li, Lin Liang, Yanhong Zhou, and Yanling Li
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circRNA ,LncRNA ,microRNA ,STAT3 ,Transcription factor ,Cytology ,QH573-671 - Abstract
Abstract Signal transducer and activator of transcription 3 (STAT3), as a key node in numerous carcinogenic signaling pathways, is activated in various tumor tissues and plays important roles in tumor formation, metastasis, and drug resistance. STAT3 is considered a potential subtarget for tumor therapy. Noncoding RNA (ncRNA) is a special type of RNA transcript. Transforming from “junk” transcripts into key molecules involved in cell apoptosis, growth, and functional regulation, ncRNA has been proven to be closely related to various epithelial–mesenchymal transition and drug resistance processes in tumor cells over the past few decades. Research on the relationship between transcription factor STAT3 and ncRNAs has attracted increased attention. To date, existing reviews have mainly focused on the regulation by ncRNAs on the transcription factor STAT3; there has been no review of the regulation by STAT3 on ncRNAs. However, understanding the regulation of ncRNAs by STAT3 and its mechanism is important to comprehensively understand the mutual regulatory relationship between STAT3 and ncRNAs. Therefore, in this review, we summarize the regulation by transcription factor STAT3 on long noncoding RNA, microRNA, and circular RNA and its possible mechanisms. In addition, we provide an update on research progress on the regulation of STAT3 by ncRNAs. This will provide a new perspective to comprehensively understand the regulatory relationship between transcription factor STAT3 and ncRNAs, as well as targeting STAT3 or ncRNAs to treat diseases such as tumors.
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- 2024
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5. Spinal apolipoprotein E is involved in inflammatory pain via regulating lipid metabolism and glial activation in the spinal dorsal horn
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Siyi Liu, Shuting Yang, Xuan Zhu, Xiang Li, Xi Zhang, Xiaoqiong Zhou, Hong Cheng, Fu-Quan Huo, Qingxiang Mao, and Lingli Liang
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Apolipoprotein E ,Astrocyte ,Spinal dorsal horn ,Complete Freund's adjuvant ,Inflammatory pain ,Biology (General) ,QH301-705.5 - Abstract
Abstract Introduction Inflammation and nerve injury promote astrocyte activation, which regulates the development and resolution of pain, in the spinal dorsal horn. APOE regulates lipid metabolism and is predominantly expressed in the astrocytes. However, the effect of astrocytic APOE and lipid metabolism on spinal cellular function is unclear. This study aimed to investigate the effect of spinal Apoe on spinal cellular functions using the complete Freund's adjuvant (CFA)-induced inflammatory pain mouse model. Methods After intraplantar injection of CFA, we assessed pain behaviors in C57BL6 and Apoe knockout (Apoe −/− ) mice using von Frey and Hargreaves’ tests and analyzed dorsal horn samples (L4-5) using western blotting, immunofluorescence, quantitative real-time polymerase chain reaction, and RNA sequencing. Results The Apoe levels were markedly upregulated at 2 h and on days 1 and 3 post-CFA treatment. Apoe was exclusively expressed in the astrocytes. Apoe −/− mice exhibited decreased pain on day 1, but not at 2 h, post-CFA treatment. Apoe −/− mice also showed decreased spinal neuron excitability and paw edema on day 1 post-CFA treatment. Global transcriptomic analysis of the dorsal horn on day 1 post-CFA treatment revealed that the differentially expressed mRNAs in Apoe −/− mice were associated with lipid metabolism and the immune system. Astrocyte activation was impaired in Apoe −/− mice on day 1 post-CFA treatment. The intrathecal injection of Apoe antisense oligonucleotide mitigated CFA-induced pain hypersensitivity. Conclusions Apoe deficiency altered lipid metabolism in astrocytes, exerting regulatory effects on immune response, astrocyte activation, and neuronal activity and consequently disrupting the maintenance of inflammatory pain after peripheral inflammation. Targeting APOE is a potential anti-nociception and anti-inflammatory strategy.
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- 2023
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6. Effects of sputum bacillary load and age on GeneXpert and traditional methods in pulmonary tuberculosis: a 4-year retrospective comparative study
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Kui Li, Qianqian Hu, Jun Liu, Siyi Liu, and Yingli He
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Bacillary load ,Age factors ,GeneXpert® MTB/RIF ,Tuberculosis ,Comparative study ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background The purpose of this study was to evaluate the diagnostic value of the GeneXpert® MTB/RIF (Xpert®), Auramine O staining method, and Lowenstein-Jensen medium for bacteriologically confirmed pulmonary tuberculosis and explore the effects of the sputum bacillary load (SBL) and qRT‒PCR threshold cycle (Ct) value on the detection methods. Methods We retrospectively analysed the results in the Department of Infectious Disease for 49 months. The χ2 test was used to compare the performances of each method, receiver operating characteristic curve analysis was used to determine the optimal cut-off values, and the factors associated with a false-negative result from Xpert® were analysed by logistic regression. Results Simultaneous analysis of 980 sputum specimens showed that the positive detection rate of Xpert® did not increase with increasing SBL, and there were differences between the three when SBL ≤ 1 + (all P 34.1 cycles. Conclusions Xpert® was not affected by SBL but it was by age, and it is more advantageous when SBL ≤ 1 + . The results regarding rifampicin resistance were reliable, and the novel Ct segmentation was a practical and more clinically meaningful classification method for diagnosing rifampicin resistance. These findings will help improve physicians’ ability to accurately diagnose TB.
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- 2023
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7. Elevational Patterns of Soil Nitrogen Forms and Transformations in the Southeastern Qinghai–Xizang Plateau
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Siyi Liu, Chuanhong Li, Lin Zhang, Yi Cheng, Zhiming Zhang, Fang He, Baomin Yao, Lili Han, Yuan Ge, Baodong Chen, Guoxin Sun, Congcong Shen, and Limei Zhang
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Ecology ,QH540-549.5 - Abstract
Alpine forest soil in the southeastern Qinghai–Xizang Plateau plays a crucial role in regional and global climate and biogeochemical cycles, yet the elevational distribution of soil nitrogen (N) availability and losing risk is unresolved. In this study, we characterized soil N composition and key N transformation processes across different elevations in 3 typical mounts of the Qinghai–Xizang Plateau. In contrast to previous suggestions, soil total N and ammonium are found to accumulate in high elevation zones. This accumulation of N at higher altitudes is due to a consistent soil net N mineralization rate coupled with an extremely low net nitrification rate, which is suppressed by low pH and high soil moisture in high elevation zones. Moreover, the elevated rates of biological N fixation along the elevation further contribute to N accumulation in high elevation regions in which the acid-tolerant Bradyrhizobium, plant-associated Herbaspirillum, and Klebsiella are identified as the key diazotrophic microbial taxa responsible for active N fixation. Collectively, our results suggest that total N and NH4+-N accumulation in higher altitude zone is a ubiquitous phenomenon in the southeast Qinghai–Xizang Plateau, with lower nitrification rates and higher biological nitrogen fixation being key processes enabling this occurrence.
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- 2024
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8. A Novel Role for the Longevity-Associated Protein SLC39A11 as a Manganese Transporter
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Zhidan Xia, Biyao Tang, Xiaopeng Li, Xinran Li, Yangfan Jia, Jianwei Jiang, Jingyao Chen, Jingshu Song, Siyi Liu, Junxia Min, and Fudi Wang
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Science - Abstract
The identification of aging- and longevity-associated genes is important for promoting healthy aging. By analyzing a large cohort of Chinese centenarians, we previously found that single-nucleotide polymorphisms (SNPs) in the SLC39A11 gene (also known as ZIP11) are associated with longevity in males. However, the function of the SLC39A11 protein remains unclear. Here, we found that SLC39A11 expression is significantly reduced in patients with Hutchinson–Gilford progeria syndrome (HGPS). In addition, we found that zebrafish with a mutation in slc39a11 that significantly reduces its expression have an accelerated aging phenotype, including a shortened average lifespan, muscle atrophy and reduced swimming, impaired muscle regeneration, gut damage, and abnormal morphology in the reproductive system. Interestingly, these signs of premature aging were more pronounced in male zebrafish than in females. RNA-sequencing analysis revealed that cellular senescence may serve as a potential mechanism for driving this slc39a11 deficiency-induced phenotype in mutant zebrafish. Moreover, immunofluorescence showed significantly increased DNA damage and reactive oxygen species signaling in slc39a11 mutant zebrafish. Using inductively coupled plasma mass spectrometry (ICP-MS), we found that manganese significantly accumulates in slc39a11 mutant zebrafish, as well as in the serum of both global Slc39a11 knockout and hepatocyte-specific Slc39a11 knockout mice, suggesting that this metal transporter regulates systemic manganese levels. Finally, using cultured human fibroblasts, we found that both knocking down SLC39A11 and exposure to high extracellular manganese increased cellular senescence. These findings provide compelling evidence that SLC39A11 serves to protect against the aging process, at least in part by regulating cellular manganese homeostasis.
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- 2024
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9. Dynamic immune landscape in vaccinated-BA.5-XBB.1.9.1 reinfections revealed a 5-month protection-duration against XBB infection and a shift in immune imprintingResearch in context
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Tingting Cui, Xiaoling Su, Jing Sun, Siyi Liu, Mingzhu Huang, Weidong Li, Chengna Luo, Li Cheng, Rui Wei, Tao Song, Xi Sun, Qi Luo, Juan Li, Jie Su, Shidong Deng, Jincun Zhao, Zhuxiang Zhao, Nanshan Zhong, and Zhongfang Wang
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SARS-CoV-2 ,Vaccinated-BA.5-XBB.1.9.1 reinfections ,Immune imprinting ,Protection-duration ,Medicine ,Medicine (General) ,R5-920 - Abstract
Summary: Background: The impact of previous vaccination on protective immunity, duration, and immune imprinting in the context of BA.5-XBB.1.9.1 reinfection remains unknown. Methods: Based on a 2-year longitudinal cohort from vaccination, BA.5 infection and XBB reinfection, several immune effectors, including neutralizing antibodies (Nabs), antibody-dependent cellular cytotoxicity (ADCC), virus-specific T cell immunity were measured to investigate the impact of previous vaccination on host immunity induced by BA.5 breakthrough infection and BA.5-XBB.1.9.1 reinfection. Findings: In absence of BA.5 Nabs, plasma collected 3 months after receiving three doses of inactivated vaccine (I-I-I) showed high ADCC that protected hACE2-K18 mice from fatality and significantly reduced viral load in the lungs and brain upon BA.5 challenge, compared to plasma collected 12 months after I-I-I. Nabs against XBB.1.9.1 induced by BA.5 breakthrough infection were low at day 14 and decreased to a GMT of 10 at 4 months and 28% (9/32) had GMT ≤4, among whom 67% (6/9) were reinfected with XBB.1.9.1 within 1 month. However, 63% (20/32) were not reinfected with XBB.1.9.1 at 5 months post BA.5 infection. Interestingly, XBB.1.9.1 reinfection increased Nabs against XBB.1.9.1 by 24.5-fold at 14 days post-reinfection, which was much higher than that against BA.5 (7.3-fold) and WT (4.5-fold), indicating an immune imprinting shifting from WT to XBB antigenic side. Interpretation: Overall, I-I-I can provide protection against BA.5 infection and elicit rapid immune response upon BA.5 infection. Furthermore, BA.5 breakthrough infection effectively protects against XBB.1.9.1 lasting more than 5 months, and XBB.1.9.1 reinfection results in immune imprinting shifting from WT antigen induced by previous vaccination to the new XBB.1.9.1 antigen. These findings strongly suggest that future vaccines should target variant strain antigens, replacing prototype strain antigens. Funding: This study was supported by R&D Program of Guangzhou National Laboratory (SRPG23-005), National Key Research and Development Program of China (2022YFC2604104, 2019YFC0810900), S&T Program of Guangzhou Laboratory (SRPG22-006), and National Natural Science Foundation of China (81971485, 82271801, 81970038), Emergency Key Program of Guangzhou Laboratory (EKPG21-30-3), Zhongnanshan Medical Foundation of Guangdong Province (ZNSA-2020013), and State Key Laboratory of Respiratory Disease (J19112006202304).
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- 2024
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10. Robust analyzes for longitudinal clinical trials with missing and non-normal continuous outcomes
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Siyi Liu, Yilong Zhang, Gregory T. Golm, Guanghan (Frank) Liu, and Shu Yang
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longitudinal clinical trial ,missing data ,multiple imputation ,robust regression ,sensitivity analysis ,Probabilities. Mathematical statistics ,QA273-280 - Abstract
Missing data is unavoidable in longitudinal clinical trials, and outcomes are not always normally distributed. In the presence of outliers or heavy-tailed distributions, the conventional multiple imputation with the mixed model with repeated measures analysis of the average treatment effect (ATE) based on the multivariate normal assumption may produce bias and power loss. Control-based imputation (CBI) is an approach for evaluating the treatment effect under the assumption that participants in both the test and control groups with missing outcome data have a similar outcome profile as those with an identical history in the control group. We develop a robust framework to handle non-normal outcomes under CBI without imposing any parametric modeling assumptions. Under the proposed framework, sequential weighted robust regressions are applied to protect the constructed imputation model against non-normality in the covariates and the response variables. Accompanied by the subsequent mean imputation and robust model analysis, the resulting ATE estimator has good theoretical properties in terms of consistency and asymptotic normality. Moreover, our proposed method guarantees the analysis model robustness of the ATE estimation in the sense that its asymptotic results remain intact even when the analysis model is misspecified. The superiority of the proposed robust method is demonstrated by comprehensive simulation studies and an AIDS clinical trial data application.
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- 2023
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11. Targeting the cochlin/SFRP1/CaMKII axis in the ocular posterior pole prevents the progression of nonpathologic myopia
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Chao Geng, Siyi Liu, Jindan Wang, Sennan Wang, Weiran Zhang, Hua Rong, Yunshan Cao, Shuqing Wang, Zhiqing Li, and Yan Zhang
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Biology (General) ,QH301-705.5 - Abstract
Abstract Myopia is a major public health issue. However, interventional modalities for nonpathologic myopia are limited due to its complicated pathogenesis and the lack of precise targets. Here, we show that in guinea pig form-deprived myopia (FDM) and lens-induced myopia (LIM) models, the early initiation, phenotypic correlation, and stable maintenance of cochlin protein upregulation at the interface between retinal photoreceptors and retinal pigment epithelium (RPE) is identified by a proteomic analysis of ocular posterior pole tissues. Then, a microarray analysis reveals that cochlin upregulates the expression of the secreted frizzled-related protein 1 (SFRP1) gene in human RPE cells. Moreover, SFRP-1 elevates the intracellular Ca2+ concentration and activates Ca2+/calmodulin-dependent protein kinase II (CaMKII) signaling in a simian choroidal vascular endothelial cell line, and elicits vascular endothelial cell dysfunction. Furthermore, genetic knockdown of the cochlin gene and pharmacological blockade of SFRP1 abrogates the reduced choroidal blood perfusion and prevents myopia progression in the FDM model. Collectively, this study identifies a novel signaling axis that may involve cochlin in the retina, SFRP1 in the RPE, and CaMKII in choroidal vascular endothelial cells and contribute to the pathogenesis of nonpathologic myopia, implicating the potential of cochlin and SFRP1 as myopia interventional targets.
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- 2023
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12. Special representative actions, the insurance value of audits, and investor protection: an empirical study based on the ruling against Kangmei Pharmaceutical Co. Ltd
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Daoguang Yang, Hongling Han, Yidan Mao, and Siyi Liu
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Special representative actions ,investor protection ,insurance hypothesis ,new Securities Law ,Accounting. Bookkeeping ,HF5601-5689 ,Finance ,HG1-9999 - Abstract
ABSTRACTThe ruling against the Kangmei Pharmaceutical Co., Ltd’s financial fraud was the first special representative action under China’s new Securities Law, serving as a milestone in investor protection in China. Exploiting the natural experiment provided by the ruling, we empirically test the insurance value of audits and find a negative market reaction of the client firms audited by the accounting firms that were still being sued as of the date of Kangmei ruling (i.e., the sued accounting firms). Further, this reaction is weakened when the solvency of the sued accounting firms or the client firms is high, and is intensified when the client firms have high litigation risk. The ruling also eroded investors’ trust in the financial statements for the year 2020 that were audited by the sued accounting firms. Overall, we provide important evidence on the effect of special representative actions from the perspective of audit insurance hypothesis.
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- 2023
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13. Impact of Microclimate on Perception and Physical Activities in Public Spaces of New Urban Areas in Beijing, China
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Mo Han, Yani Fang, Li Yi, and Siyi Liu
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microclimate ,public space in new urban areas ,physical activity ,spatial features ,post-occupancy evaluation ,Building construction ,TH1-9745 - Abstract
The development of new urban areas in Beijing has alleviated overcrowding in old urban centers and has ample public spaces for recreational activities. However, these public spaces are not ideally designed and have not been as successful as expected. Few studies have investigated the ineffective use of these public spaces in terms of microclimatic and thermal comfort factors. Our study investigated microclimatic factors, the subjective assessment of thermal comfort, the intensity of human activities, and the spatial features of public spaces in conjunction with surrounding buildings in a mixed commercial and residential complex in the Fangshan District, Beijing. We used a mixed-methods approach comprising microclimate measurements, questionnaires (n = 150), spatiotemporal behavior mapping, and field measurements. Our results showed that the human perception of the microclimate is related to the exposure duration and other microclimatic factors. The perception of people who spend longer periods outdoors is often inconsistent with objectively measured thermal comfort values. Activity intensity (low, medium, and high) was also related to the duration of time spent outdoors. Microclimatic factors affect the number of people at different activity intensities and the trajectory of the activities. Different spatial features cause different microclimate formations and can directly influence the human subjective assessment of thermal comfort. This study uniquely links the microclimate to human perceptions, physical activities, and spatial features in service of redesigning public spaces. We developed a comprehensive methodology that expands the post-occupancy evaluation and proposes new urban public space designs that consider microclimates. This study also provides a new perspective for promoting physical activity by enhancing the thermal comfort of the environment to achieve physical and mental health goals.
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- 2024
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14. Genomic prediction in pigs using data from a commercial crossbred population: insights from the Duroc x (Landrace x Yorkshire) three-way crossbreeding system
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Siyi Liu, Tianxiong Yao, Dong Chen, Shijun Xiao, Liqing Chen, and Zhiyan Zhang
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Animal culture ,SF1-1100 ,Genetics ,QH426-470 - Abstract
Abstract Background Genomic selection is widely applied for genetic improvement in livestock crossbreeding systems to select excellent nucleus purebred (PB) animals and to improve the performance of commercial crossbred (CB) animals. Most current predictions are based solely on PB performance. Our objective was to explore the potential application of genomic selection of PB animals using genotypes of CB animals with extreme phenotypes in a three-way crossbreeding system as the reference population. Using real genotyped PB as ancestors, we simulated the production of 100,000 pigs for a Duroc x (Landrace x Yorkshire) DLY crossbreeding system. The predictive performance of breeding values of PB animals for CB performance using genotypes and phenotypes of (1) PB animals, (2) DLY animals with extreme phenotypes, and (3) random DLY animals for traits of different heritabilities ( $${h}^{2}$$ h 2 = 0.1, 0.3, and 0.5) was compared across different reference population sizes (500 to 6500) and prediction models (genomic best linear unbiased prediction (GBLUP) and Bayesian sparse linear mixed model (BSLMM)). Results Using a reference population consisting of CB animals with extreme phenotypes showed a definite predictive advantage for medium- and low-heritability traits and, in combination with the BSLMM model, significantly improved selection response for CB performance. For high-heritability traits, the predictive performance of a reference population of extreme CB phenotypes was comparable to that of PB phenotypes when the effect of the genetic correlation between PB and CB performance ( $${r}_{pc}$$ r pc ) on the accuracy obtained with a PB reference population was considered, and the former could exceed the latter if the reference size was large enough. For the selection of the first and terminal sires in a three-way crossbreeding system, prediction using extreme CB phenotypes outperformed the use of PB phenotypes, while the optimal design of the reference group for the first dam depended on the percentage of individuals from the corresponding breed that the PB reference data comprised and on the heritability of the target trait. Conclusions A commercial crossbred population is promising for the design of the reference population for genomic prediction, and selective genotyping of CB animals with extreme phenotypes has the potential for maximizing genetic improvement for CB performance in the pig industry.
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- 2023
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15. Dissecting the molecular trajectory of fibroblast reprogramming to chemically induced mammary epithelial cells
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Liangshan Qin, Dandan Zhang, Siyi Liu, Quanhui Liu, Mingxing Liu, and Ben Huang
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reprogramming ,small-molecule compounds ,induced mammary epithelial cells ,molecular trajectory ,transdifferentiation ,Biology (General) ,QH301-705.5 - Abstract
Introduction: The plasticity of cell identity allows cellular reprogramming that manipulates the lineage of cells to generate the target cell types, bringing new avenues for disease modeling and autologous tailored cell therapy. Previously, we had already successfully established a technical platform for inducing fibroblast reprogramming to chemically induced mammary epithelial cells (CiMECs) by small-molecule compounds. However, exactly how the molecular mechanism driving the lineage conversion remains unknown.Methods: We employ the RNA-sequencing technology to investigate the transcriptome event during the reprogramming process and reveal the molecular mechanisms for the fate acquisition of mammary lineage.Results: The multi-step reprogramming process first overcomes multiple barriers, including the inhibition of mesenchymal characteristics, pro-inflammatory and cell death signals, and then enters an intermediate plastic state. Subsequently, the hormone and mammary development genes were rapidly activated, leading to the acquisition of the mammary program together with upregulation of the milk protein synthesis signal. Moreover, the gene network analyses reveal the potential relationship between the TGF-β signaling pathway to mammary lineage activation, and the changes in the expression of these genes may play important roles in coordinating the reprogramming process.Conclusion: Together, these findings provide critical insights into the molecular route and mechanism triggered by small-molecule compounds that induce fibroblast reprogramming into the fate of mammary epithelial cells, and they also laid a foundation for the subsequent research on the development and differentiation of mammary epithelial cells and lactation.
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- 2023
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16. The Evolution of Primate Short-Term Memory
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ManyPrimates, Géraud Aguenounon, Matthias Allritz, Drew M. Altschul, Sébastien Ballesta, Alice Beaud, Manuel Bohn, Sally L. Bornbusch, Angela Brandão, James Brooks, Thomas Bugnyar, Judith M. Burkart, Léa Bustamante, Josep Call, Charlotte Canteloup, Chuangshi Cao, Kai R. Caspar, Diana da Silva, Alexandra A. de Sousa, Sarah E. DeTroy, Shona Duguid, Timothy M. Eppley, Claudia Fichtel, Julia Fischer, Chi Gong, James A. Grange, Nicholas M. Grebe, Daniel Hanus, Daniel Haun, Lou M. Haux, Yseult Héjja-Brichard, Annabella Helman, Istvan Hernadi, R. Adriana Hernandez-Aguilar, Esther Herrmann, Lydia M. Hopper, Lauren H. Howard, Lei Huang, Sarah M. Huskisson, Ivo Jacobs, Zhiyong Jin, Marine Joly, Fumihiro Kano, Stefanie Keupp, Evelin Kiefer, Balázs Knakker, Katalin Kóczán, Larissa Kraus, Sze Chai Kwok, Marie Lefrançois, Laura Lewis, Siyi Liu, Miquel Llorente, Elizabeth Lonsdorf, Louise Loyant, Katarzyna Majecka, Luke Maurits, Hélène Meunier, Flávia Mobili, Luca Morino, Alba Motes-Rodrigo, Vincent Nijman, Caroline Nkov Ihomi, Tomas Persson, Dariusz Pietraszewski, Juan Felipe Reátiga Parrish, Anthony Roig, Alejandro Sánchez-Amaro, Yutaro Sato, Gabriela-Alina Sauciuc, Allie E. Schrock, Manon K. Schweinfurth, Amanda Seed, Caroline L. Shearer, Vedrana Šlipogor, Yanjie Su, Kirsten Sutherland, Jingzhi Tan, Derry Taylor, Camille A. Troisi, Christoph J. Völter, Elizabeth Warren, Julia Watzek, and Pauline Zablocki-Thomas
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cognitive evolution ,short-term memory ,primate cognition ,phylogenetic analysis ,Zoology ,QL1-991 - Abstract
Short-term memory is implicated in a range of cognitive abilities and is critical for understanding primate cognitive evolution. To investigate the effects of phylogeny, ecology and sociality on short-term memory, we tested the largest and most diverse primate sample to date (421 non-human primates across 41 species) in an experimental delayed-response task. Our results confirm previous findings that longer delays decrease memory performance across species and taxa. Our analyses demonstrate a considerable contribution of phylogeny over ecological and social factors on the distribution of short-term memory performance in primates; closely related species had more similar short-term memory abilities. Overall, individuals in the branch of Hominoidea performed better compared to Cercopithecoidea, who in turn performed above Platyrrhini and Strepsirrhini. Interdependencies between phylogeny and socioecology of a given species presented an obstacle to disentangling the effects of each of these factors on the evolution of short-term memory capacity. However, this study offers an important step forward in understanding the interspecies and individual variation in short-term memory ability by providing the first phylogenetic reconstruction of this trait’s evolutionary history. The dataset constitutes a unique resource for studying the evolution of primate cognition and the role of short-term memory in other cognitive abilities.
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- 2022
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17. Physiological, Cytological, and Transcriptomic Analysis of Magnesium Protoporphyrin IX Methyltransferase Mutant Reveal Complex Genetic Regulatory Network Linking Chlorophyll Synthesis and Chloroplast Development in Rice
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Youming Yao, Hongyu Zhang, Rong Guo, Jiangmin Fan, Siyi Liu, Jianglin Liao, Yingjin Huang, and Zhaohai Wang
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magnesium protoporphyrin IX methyltransferase (ChlM) ,chlorophyll synthesis ,chloroplast development ,hormone ,rice ,Botany ,QK1-989 - Abstract
Functional defects in key genes for chlorophyll synthesis usually cause abnormal chloroplast development, but the genetic regulatory network for these key genes in regulating chloroplast development is still unclear. Magnesium protoporphyrin IX methyltransferase (ChlM) is a key rate-limiting enzyme in the process of chlorophyll synthesis. Physiological analysis showed that the chlorophyll and carotenoid contents were significantly decreased in the chlm mutant. Transmission electron microscopy demonstrated that the chloroplasts of the chlm mutant were not well developed, with poor, loose, and indistinct thylakoid membranes. Hormone content analysis found that jasmonic acid, salicylic acid, and auxin accumulated in the mutant. A comparative transcriptome profiling identified 1534 differentially expressed genes (DEGs) between chlm and the wild type, including 876 up-regulated genes and 658 down-regulated genes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that these DEGs were highly involved in chlorophyll metabolism, chloroplast development, and photosynthesis. Protein−protein interaction network analysis found that protein translation played an essential role in the ChlM gene-regulated process. Specifically, 62 and 6 DEGs were annotated to regulate chlorophyll and carotenoid metabolism, respectively; 278 DEGs were predicted to be involved in regulating chloroplast development; 59 DEGs were found to regulate hormone regulatory pathways; 192 DEGs were annotated to regulate signal pathways; and 49 DEGs were putatively identified as transcription factors. Dozens of these genes have been well studied and reported to play essential roles in chlorophyll accumulation or chloroplast development, providing direct evidence for the reliability of the role of the identified DEGs. These findings suggest that chlorophyll synthesis and chloroplast development are actively regulated by the ChlM gene. And it is suggested that hormones, signal pathways, and transcription regulation were all involved in these regulation processes. The accuracy of transcriptome data was validated by quantitative real-time PCR (qRT-PCR) analysis. This study reveals a complex genetic regulatory network of the ChlM gene regulating chlorophyll synthesis and chloroplast development. The ChlM gene’s role in retrograde signaling was discussed. Jasmonic acid, salicylic acid, or their derivatives in a certain unknown state were proposed as retrograde signaling molecules in one of the signaling pathways from the chloroplast to nucleus.
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- 2023
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18. Yellow-Green Leaf 19 Encoding a Specific and Conservative Protein for Photosynthetic Organisms Affects Tetrapyrrole Biosynthesis, Photosynthesis, and Reactive Oxygen Species Metabolism in Rice
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Qiang Wang, Hongyu Zhang, Lingxia Wei, Rong Guo, Xuanzhi Liu, Miao Zhang, Jiangmin Fan, Siyi Liu, Jianglin Liao, Yingjin Huang, and Zhaohai Wang
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yellow-green leaf 19 (ygl19) ,tetrapyrrole biosynthesis ,photosynthesis ,ROS metabolism ,rice (Oryza sativa) ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Chlorophyll is the main photosynthetic pigment and is crucial for plant photosynthesis. Leaf color mutants are widely used to identify genes involved in the synthesis or metabolism of chlorophyll. In this study, a spontaneous mutant, yellow-green leaf 19 (ygl19), was isolated from rice (Oryza sativa). This ygl19 mutant showed yellow-green leaves and decreased chlorophyll level and net photosynthetic rate. Brown necrotic spots appeared on the surface of ygl19 leaves at the tillering stage. And the agronomic traits of the ygl19 mutant, including the plant height, tiller number per plant, and total number of grains per plant, were significantly reduced. Map-based cloning revealed that the candidate YGL19 gene was LOC_Os03g21370. Complementation of the ygl19 mutant with the wild-type CDS of LOC_Os03g21370 led to the restoration of the mutant to the normal phenotype. Evolutionary analysis revealed that YGL19 protein and its homologues were unique for photoautotrophs, containing a conserved Ycf54 functional domain. A conserved amino acid substitution from proline to serine on the Ycf54 domain led to the ygl19 mutation. Sequence analysis of the YGL19 gene in 4726 rice accessions found that the YGL19 gene was conserved in natural rice variants with no resulting amino acid variation. The YGL19 gene was mainly expressed in green tissues, especially in leaf organs. And the YGL19 protein was localized in the chloroplast for function. Gene expression analysis via qRT-PCR showed that the expression levels of tetrapyrrole synthesis-related genes and photosynthesis-related genes were regulated in the ygl19 mutant. Reactive oxygen species (ROS) such as superoxide anions and hydrogen peroxide accumulated in spotted leaves of the ygl19 mutant at the tillering stage, accompanied by the regulation of ROS scavenging enzyme-encoding genes and ROS-responsive defense signaling genes. This study demonstrates that a novel yellow-green leaf gene YGL19 affects tetrapyrrole biosynthesis, photosynthesis, and ROS metabolism in rice.
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- 2023
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19. Case report: Acute right ventricular dysfunction after surgery in a pregnant patient with congenital heart disease and aortic dissection
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Junhai Hao, Siyi Liu, Tucheng Sun, and Liming Lei
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acute right ventricular dysfunction ,cardiac surgery ,aortic dissection ,pregnant patient ,congenital heart disease ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Pregnant women with aortic dissection are hemodynamically outmost complex patients. The two major diagnoses that should be considered in pregnant patients with congenital heart disease (CHD) and acute type A aortic dissection presenting with postoperative right ventricular dysfunction are pulmonary thromboembolism and right ventricular infarction. We present a rare case of postoperative right ventricular dysfunction in pregnant women with CHD and acute aortic dissection, which was diagnosed by pulmonary computed tomography angiography and treated by percutaneous coronary intervention.
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- 2023
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20. Research on Lightweight Model for Rapid Identification of Chunky Food Based on Machine Vision
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Zhongfeng Guo, Junlin Yang, and Siyi Liu
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deep learning ,YOLOv5 algorithm ,lightweight model ,machine vision ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
To meet the demands of the food industry for automatic sorting of block-shaped foods using DELTA robots, a machine vision detection method capable of quickly identifying such foods needs to be studied. This paper proposes a lightweight model that incorporates the CBAM attention mechanism into the YOLOv5 model, replaces ordinary convolution with ghost convolution, and replaces the position loss function with SIoU loss. The resulting YOLOv5-GCS model achieves a mAP increase from 95.4% to 97.4%, and a reduction in parameter volume from 7.0 M to 6.2 M, compared to the YOLOv5 model. Furthermore, the first 17 layers of the MobileNetv3-large network are replaced with the CSPDarkNet53 network in YOLOv5-GCS, resulting in the YOLOv5-MGCS lightweight model, with a high FPS of 83, which is capable of fast identification of block-shaped foods.
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- 2023
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21. Quantitative, multiplexed, targeted proteomics for ascertaining variant specific SARS-CoV-2 antibody response
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Ivan Doykov, Tomas Baldwin, Justyna Spiewak, Kimberly C. Gilmour, Joseph M. Gibbons, Corinna Pade, Catherine J. Reynolds, Áine McKnight, Mahdad Noursadeghi, Mala K. Maini, Charlotte Manisty, Thomas Treibel, Gabriella Captur, Marianna Fontana, Rosemary J. Boyton, Daniel M. Altmann, Tim Brooks, Amanda Semper, James C. Moon, Kevin Mills, Wendy E. Heywood, Hakam Abbass, Aderonke Abiodun, Mashael Alfarih, Zoe Alldis, Oliver E. Amin, Mervyn Andiapen, Jessica Artico, João B. Augusto, Georgina L. Baca, Sasha N.L. Bailey, Anish N. Bhuva, Alex Boulter, Ruth Bowles, Olivia V. Bracken, Ben O’Brien, Natalie Bullock, David K. Butler, Olivia Carr, Nicola Champion, Carmen Chan, Aneesh Chandran, Tom Coleman, Jorge Couto de Sousa, Xose Couto-Parada, Eleanor Cross, Teresa Cutino-Moguel, Silvia D’Arcangelo, Rhodri H. Davies, Brooke Douglas, Cecilia Di Genova, Keenan Dieobi-Anene, Mariana O. Diniz, Anaya Ellis, Karen Feehan, Malcolm Finlay, Nasim Forooghi, Sasha Francis, David Gillespie, Derek Gilroy, Matt Hamblin, Gabrielle Harker, Georgia Hemingway, Jacqueline Hewson, Wendy Heywood, Lauren M. Hickling, Bethany Hicks, Aroon D. Hingorani, Lee Howes, Ivie Itua, Victor Jardim, Wing-Yiu Jason Lee, Melaniepetra Jensen, Jessica Jones, Meleri Jones, George Joy, Vikas Kapil, Caoimhe Kelly, Hibba Kurdi, Jonathan Lambourne, Kai-Min Lin, Siyi Liu, Aaron Lloyd, Sarah Louth, Vineela Mandadapu, Katia Menacho, Celina Mfuko, Sebastian Millward, Oliver Mitchelmore, Christopher Moon, James Moon, Diana Muñoz Sandoval, Sam M. Murray, Ashley Otter, Susana Palma, Ruth Parker, Kush Patel, Mihaela Pawarova, Steffen E. Petersen, Brian Piniera, Franziska P. Pieper, Lisa Rannigan, Alicja Rapala, Amy Richards, Matthew Robathan, Joshua Rosenheim, Cathy Rowe, Matthew Royds, Jane Sackville West, Genine Sambile, Nathalie M. Schmidt, Hannah Selman, Andreas Seraphim, Mihaela Simion, Angelique Smit, Michelle Sugimoto, Leo Swadling, Stephen Taylor, Nigel Temperton, Stephen Thomas, George D. Thornton, Thomas A. Treibel, Art Tucker, Ann Varghese, Jessry Veerapen, Mohit Vijayakumar, Tim Warner, Sophie Welch, Hannah White, Theresa Wodehouse, Lucinda Wynne, and Dan Zahedi
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complement: immunoglobulin ,SARS-CoV-2 ,vaccination ,proteomics ,COVID-19 ,variant of concern ,Biotechnology ,TP248.13-248.65 ,Biochemistry ,QD415-436 ,Science - Abstract
Summary: Determining the protection an individual has to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) variants of concern (VoCs) is crucial for future immune surveillance, vaccine development, and understanding of the changing immune response. We devised an informative assay to current ELISA-based serology using multiplexed, baited, targeted proteomics for direct detection of multiple proteins in the SARS-CoV-2 anti-spike antibody immunocomplex. Serum from individuals collected after infection or first- and second-dose vaccination demonstrates this approach and shows concordance with existing serology and neutralization. Our assays show altered responses of both immunoglobulins and complement to the Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.1) VoCs and a reduced response to Omicron (B1.1.1529). We were able to identify individuals who had prior infection, and observed that C1q is closely associated with IgG1 (r > 0.82) and may better reflect neutralization to VoCs. Analyzing additional immunoproteins beyond immunoglobulin (Ig) G, provides important information about our understanding of the response to infection and vaccination. Motivation: Assays for measuring serum antibody responses are typically limited to measurement of a total or single immunoglobulin isotype. The antibody response is far more complex, with multiple immunoglobulin classes, isotypes, and complement factors involved. This is a potential wealth of information that is typically understudied and missed by existing tests. The global COVID-19 pandemic has highlighted the need to understand better the immune response in respect to vaccine development and emerging new severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) variants. Using the ability of tandem mass spectrometry to multiplex and directly and accurately measure the antibody complex, we devised an alternative assay to capture this valuable information.
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- 2022
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22. Gray matter volume alterations in subjects with overweight and obesity: Evidence from a voxel-based meta-analysis
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Lei Li, Hua Yu, Ming Zhong, Siyi Liu, Wei Wei, Yajing Meng, Ming-li Li, Tao Li, and Qiang Wang
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overweight and obesity ,BMI ,gray matter volume ,voxel-based morphometry ,meta-analysis ,Psychiatry ,RC435-571 - Abstract
BackgroundObesity is a multi-systemic disease with complex etiology. And consistent evidence indicated obesity or overweight subjects render brain structure changes. Increasing evidence indicates these subjects have shown widespread structural brain gray matter volume (GMV) changes. However, results from other neuroimaging studies have been inconsistent. Consequently, the question remains whether body mass index (BMI), a gold standard to define obesity/overweight, is associated with brain structural changes.MethodsThis study will apply an updated meta-analysis of voxel-based GMV studies to compare GMV changes in overweight and obese subjects. Online databases were used to build on relevant studies published before May 2022. The updated Seed-based d Mapping with Permutation of Subject Images (SDM-PSI) explores GMV changes in individuals with overweight and obesity and further examines the correlation between GMV and obesity-related variables, specifically body mass index (BMI).ResultsThis research included fourteen studies and provided a whole-brain analysis of GMV distribution in overweight and obese individuals. It revealed lower GMV in brain regions, including the left putamen and right precentral gyrus, in individuals with overweight and obesity compared to lean controls. Further, meta-regression analyses revealed GMV in the left middle occipital gyrus was negatively correlated with the BMI of the whole sample.ConclusionGMV decreased was reported in reward circuit processing areas and sensorimotor processing areas of individuals with overweight and obesity diagnoses, suggesting an underlying structural basis for reward processing and sensorimotor processing dysregulation in overweight and obese subjects. Our results also suggest that GMV in occipital gyrus, a key region for food visual and gustatory encoding, is negatively associated with BMI. These results provide further evidence for the dysregulated reward circuit in individuals with overweight and obesity.
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- 2022
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23. An outbreak of extensively drug-resistant and hypervirulent Klebsiella pneumoniae in an intensive care unit of a teaching hospital in Southwest China
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Siyi Liu, Yinhuan Ding, Yifei Xu, Zhaoyinqian Li, Zhangrui Zeng, and Jinbo Liu
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Klebsiella pneumoniae ,extensively drug-resistant ,hypervirulent ,tigecycline resistance ,outbreak ,Microbiology ,QR1-502 - Abstract
Extensively drug-resistant and hypervirulent Klebsiella pneumoniae (XDR-hvKp) is a new problem for patients in Intensive Care Unit (ICU) and can become an even more severe threat if resistant to tigecycline, considered one of the ‘last lines of defense’ drugs. This study collected seven non-replicated tigecycline-resistant XDR-hvKp from seven patients and performed genome analysis and epidemiological investigation using whole genome equencing (WGS) and other methods. All strains in this study were identified as ST11-KL64 and showed high resistance to antibiotics such as β-lactams, aminoglycosides, quinolones, and tigecycline, and one strain was also resistant to colistin. All strains were determined to be hvKp by the results of serum resistance assay and Galleria mellonella infection models. All strains had resistance genes blaCTX-M-65,blaKPC-2,blaLAP-2,blaTEM-1B, rmtB, and qnrS1 and virulence factors such as rmpA, rmpA2, and aerobactin (iucABCD, iutA). The expression of the AcrAB-TolC efflux pump was upregulated in all strains, and the expression levels of the gene pmrK was significantly upregulated in colistin-resistant strain DP compared to colistin-sensitive strain WT in this study. In conclusion, we described an outbreak caused by tigecycline-resistant XDR-hvKp in the ICU of a teaching hospital in southwest China. The spread of these superbugs poses a great threat to patients and therefore requires us to closely monitor these XDR-hvKp and develop relevant strategies to combat them.
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- 2022
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24. The gene regulatory role of non-coding RNAs in non-obstructive azoospermia
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Guanqing Zhou, Mimi Zhang, Jingzhi Zhang, Yaofeng Feng, Zhishen Xie, Siyi Liu, Detu Zhu, and Yumei Luo
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non-obstructive azoospermia ,non-coding RNA ,microRNA ,PIWI-interacting RNA ,long non-coding RNA ,circular RNA ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
Non-coding RNAs are classified as small non-coding RNAs, long non-coding RNAs and circular RNAs, which are involved in a variety of biological processes, including cell differentiation, proliferation, apoptosis and pathological conditions of various diseases. Many studies have shown that non-coding RNAs are related to spermatogenesis, maturation, apoptosis, function, etc. In addition, the expression of non-coding RNAs in testicular tissue and semen of patients with non-obstructive azoospermia was different. However, the role of non-coding RNAs in the pathogenesis of non-obstructive azoospermia has not been fully elucidated, and the role of non-coding RNAs in non-obstructive azoospermia is rarely reviewed. Here we summarize the research progress of non-coding RNAs in the pathogenesis of non-obstructive azoospermia.
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- 2022
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25. Identification of AP2/ERF transcription factors in Tetrastigma hemsleyanum revealed the specific roles of ERF46 under cold stress
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Zhuomi Xie, Chuyun Yang, Siyi Liu, Mingjie Li, Li Gu, Xin Peng, and Zhongyi Zhang
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Tetrastigma hemsleyanum Diels at Gilg ,cold stress ,expression profiling ,AP2/ERF transcription factor (TFs) ,functional analysis (FA) ,Plant culture ,SB1-1110 - Abstract
Tetrastigma hemsleyanum (T. hemsleyanum) is a traditional medicinal plant that is widely used in China. Cultivated T. hemsleyanum usually encounters cold stress, limiting its growth and quality at key developmental stages. APETALA2 (AP2)/ethylene-responsive factor (ERF) transcription factors (TFs) comprise one of the largest gene superfamilies in plants and are widely involved in biotic and abiotic stresses. To reveal the roles of AP2/ERF TFs during T. hemsleyanum development, 70 AP2/ERF TFs were identified in T. hemsleyanum. Among them, 18 and 2 TFs were classified into the AP2 and RAV families, respectively. The other 50 TFs belonged to the ERF family and were further divided into the ERF and (dehydration reaction element binding factor) DREB subfamilies. The ERF subfamily contained 46 TFs, while the DREB subfamily contained 4 TFs. Phylogenetic analysis indicated that AP2/ERF TFs could be classified into five groups, in which 10 conserved motifs were confirmed. Several motifs were group- or subgroup-specific, implying that they were significant for the functions of the AP2/ERF TFs of these clades. In addition, 70 AP2/ERF TFs from the five groups were used for an expression pattern analysis under three low-temperature levels, namely, –4, 0, and 4°C. The majority of these AP2/ERF TFs exhibited a positive response to cold stress conditions. Specifically, ThERF5, ThERF31, ThERF46, and ThERF55 demonstrated a more sensitive response to cold stress. Moreover, AP2/ERF TFs exhibited specific expression patterns under cold stress. Transient overexpression and RNA interference indicated that ThERF46 has a specific tolerance to cold stress. These new insights provide the basis for further studies on the roles of AP2/ERF TFs in cold stress tolerance in T. hemsleyanum.
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- 2022
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26. Predicting the Risk of Diabetic Foot Ulcers From Diabetics With Dysmetabolism: A Retrospective Clinical Trial
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Mingyang Jiang, Fu Gan, Meishe Gan, Huachu Deng, Xuxu Chen, Xintao Yuan, Danyi Huang, Siyi Liu, Baoyu Qin, Yanhong Wei, Shanggui Su, and Zhandong Bo
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diabetic foot ulcer (DFU) ,nomogram ,risk factor ,prediction model ,dysmetabolism ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
BackgroundDiabetic foot ulcer (DFU) in patients with type 2 diabetes mellitus (T2D) often leads to amputation. Early intervention to prevent DFU is urgently necessary. So far, there have been no studies on predictive models associated with DFU risk factors. Our study aimed to quantify the predictive risk value of DFU, promote health education, and further develop behavioral interventions to reduce the incidence of DFU.MethodsData from 973 consecutive patients with T2D was collected from two hospitals. Patients from the Guangxi Medical University First Affiliated Hospital formed the training cohort (n = 853), and those from the Wuming Hospital of Guangxi Medical University formed the validation cohort (n = 120). Independent variable grouping analysis and multivariate logistic regression analysis were used to determine the risk factors of DFUs. The prediction model was established according to the related risk factors. In addition, the accuracy of the model was evaluated by specificity, sensitivity, predictive value, and predictive likelihood ratio.ResultsIn total, 369 of the 853 patients (43.3%) and 60 of the 120 (50.0%) were diagnosed with DFUs in the two hospitals. The factors associated with DFU were old age, male gender, lower body mass index (BMI), longer duration of diabetes, history of foot disease, cardiac insufficiency, no use of oral hypoglycemic agent (OHA), high white blood cell count, high platelet count, low hemoglobin level, low lymphocyte absolute value, and high postprandial blood glucose. After incorporating these 12 factors, the nomogram drawn achieved good concordance indexes of 0.89 [95% confidence interval (CI): 0.87 to 0.91] in the training cohort and 0.84 (95% CI: 0.77 to 0.91) in the validation cohort in predicting DFUs and had well-fitted calibration curves. Patients who had a nomogram score of ≥180 were considered to have a low risk of DFU, whereas those having ≥180 were at high risk.ConclusionsA nomogram was constructed by combining 12 identified risk factors of DFU. These 12 risk factors are easily available in hospitalized patients, so the prediction of DFU in hospitalized patients with T2D has potential clinical significance. The model provides a reliable prediction of the risk of DFU in patients with T2D.
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- 2022
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27. Patterns of Convergence and Divergence Between Bipolar Disorder Type I and Type II: Evidence From Integrative Genomic Analyses
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Yunqi Huang, Yunjia Liu, Yulu Wu, Yiguo Tang, Mengting Zhang, Siyi Liu, Liling Xiao, Shiwan Tao, Min Xie, Minhan Dai, Mingli Li, Hongsheng Gui, and Qiang Wang
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bipolar disorder ,genome-wide association studies ,transcriptome-wide association analysis ,Mendelian randomization ,bipolar type I ,bipolar type II ,Biology (General) ,QH301-705.5 - Abstract
Aim: Genome-wide association studies (GWAS) analyses have revealed genetic evidence of bipolar disorder (BD), but little is known about the genetic structure of BD subtypes. We aimed to investigate the genetic overlap and distinction of bipolar type I (BD I) & type II (BD II) by conducting integrative post-GWAS analyses.Methods: We utilized single nucleotide polymorphism (SNP)–level approaches to uncover correlated and distinct genetic loci. Transcriptome-wide association analyses (TWAS) were then approached to pinpoint functional genes expressed in specific brain tissues and blood. Next, we performed cross-phenotype analysis, including exploring the potential causal associations between two BD subtypes and lithium responses and comparing the difference in genetic structures among four different psychiatric traits.Results: SNP-level evidence revealed three genomic loci, SLC25A17, ZNF184, and RPL10AP3, shared by BD I and II, and one locus (MAD1L1) and significant gene sets involved in calcium channel activity, neural and synapsed signals that distinguished two subtypes. TWAS data implicated different genes affecting BD I and II through expression in specific brain regions (nucleus accumbens for BD I). Cross-phenotype analyses indicated that BD I and II share continuous genetic structures with schizophrenia and major depressive disorder, which help fill the gaps left by the dichotomy of mental disorders.Conclusion: These combined evidences illustrate genetic convergence and divergence between BD I and II and provide an underlying biological and trans-diagnostic insight into major psychiatric disorders.
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- 2022
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28. Competition between Hydration Shell and Ordered Water Chain Induces Thickness-Dependent Desalination Performance in Carbon Nanotube Membrane
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Siyi Liu, Liya Wang, Jun Xia, Ruijie Wang, Chun Tang, and Chengyuan Wang
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thickness effect ,carbon nanotube ,nanoporous monolayer graphene ,ion dehydration ,desalination performance ,Chemical technology ,TP1-1185 ,Chemical engineering ,TP155-156 - Abstract
Exploring new reverse osmosis (RO) membranes that break the permeability-selectivity trade-off rule is the ultimate goal in seawater desalination. Both nanoporous monolayer graphene (NPG) and carbon nanotube (CNT) channels have been proposed to be promising candidates for this purpose. From the perspective of membrane thickness, both NPG and CNT can be classified into the same category, as NPG is equivalent to the thinnest CNT. While NPG has the advantage of a high water flux rate and CNT is excellent at salt rejection performance, a transition is expected in practical devices when the channel thickness increases from NPG to infinite-sized CNTs. By employing molecular dynamics (MD) simulations, we find that as the thickness of CNT increases, the water flux diminishes but the ion rejection rate increases. These transitions lead to optimal desalination performance around the cross-over size. Further molecular analysis reveals that this thickness effect originates from the formation of two hydration shells and their competition with the ordered water chain structure. With the increase in CNT thickness, the competition-dominated ion path through CNT is further narrowed. Once above this cross-over size, the highly confined ion path remains unchanged. Thus, the number of reduced water molecules also tends to stabilize, which explains the saturation of the salt rejection rate with the increasing CNT thickness. Our results offer insights into the molecular mechanisms of the thickness-dependent desalination performance in a one-dimensional nanochannel, which can provide useful guidance for the future design and optimization of new desalination membranes.
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- 2023
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29. Impact of Microclimate on People’s Experiences and Behaviours in the Cultural Consumption Space: A Case Study of Panjiayuan Antique Market in Beijing, China
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Mo Han, Bing Han, Siyi Liu, and Ziwen Sun
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microclimate ,urban experience ,walking behaviour ,spatiotemporal patterns ,Post-occupancy Evaluation (POE) ,Building construction ,TH1-9745 - Abstract
Antique and cultural consumption spaces make a great contribution to urban vitality where numerous people walk, stay, and trade. However, how these people’s experiences and behaviours are affected by the microclimate of such spaces has not been studied till now. To address this gap, and using the concept of Post-occupancy Evaluation (POE) as a basis, our study investigated microclimate factors, subjective experiences, and spatiotemporal behaviour patterns in Panjiayuan Antique Market in Beijing, China. Using a mixed-methods approach comprising subjective questionnaires (n = 101), face-to-face interviews (n = 81), spatiotemporal behaviour mapping (n = 8455), and on-site observations, our results showed that microclimates impact people’s experiences and behaviours, with visibility and noise being the two primary impact factors. Most female visitors are more sensitive to the microclimate than male visitors. Furthermore, vendors in the Antique Market preferred to amend their nearby environments to increase the microclimate quality to offer a better experience to visitors around them. This study developed a comprehensive methodology that expands POE in relation to microclimatic factors in the context of cultural consumption spaces. These findings suggest that microclimates have different impacts on people’s experiences and behaviours in different spaces, which should be considered when designing and renewing urban antique markets in the future.
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- 2023
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30. Application of Wavelet Transform for the Detection of Cetacean Acoustic Signals
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Ruilin He, Yang Dai, Siyi Liu, Yuhao Yang, Yingdong Wang, Wei Fan, and Shengmao Zhang
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wavelet transform ,short-time Fourier transform ,hydrophone ,Cetacean ,acoustic signal ,signal detection ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
Cetaceans are an important part of the ocean ecosystem and are widely distributed in seas across the world. Cetaceans are heavily reliant on acoustic signals for communication. Some Odontoceti can perceive their environments using their sonar system, including the detection, localization, discrimination, and recognition of objects. Acoustic signals are one of the most commonly used types of data for Cetacean research, and it is necessary to develop Cetacean acoustic signal detection methods. This study compared the performance of a manual method, short-time Fourier transform (STFT), and wavelet transform (WT) in Cetacean acoustic signal detection. The results showed that WT performs better in click detection. According to this research, we propose using STFT for whistle and burst-pulse marking and WT for click marking in dataset building. This research will be helpful in facilitating research on the habits and behaviors of groups and individuals, thus providing information to develop methods for protecting species and developing biological resources.
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- 2023
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31. The efficacy and safety of fast track surgery (FTS) in patients after hip fracture surgery: a meta-analysis
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Mingyang Jiang, Siyi Liu, Huachu Deng, Xuzhi Liang, and Zhandong Bo
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Fast track surgery (FTS) ,Enhanced recovery after surgery (ERAS) ,Hip replacement ,Meta-analysis ,Orthopedic surgery ,RD701-811 ,Diseases of the musculoskeletal system ,RC925-935 - Abstract
Abstract Background Fast track surgery (FTS) has been gradually applied in perioperative management of orthopedic surgery, but there still some research suspected that the prognosis of patients is not as expected and the cost is high, the effect of the FTS still urgently needed for support by evidence-based medicine. Methods We retrieved RCTs from medical research literature databases. Risk ratios (RR), standard mean difference (SMD), and 95% confidence intervals (CI) were calculated to compare the primary and safety endpoints. Results Overall, a total of 8886 patients were retrieved from 57 articles, of which 4448 patients (50.06%) were randomized to experimental group whereas 4438 patients (49.94%) were randomized to control group. The result showed that FTS could significantly shorten the length of stay (LOS), decrease the visual analog scale (VAS), reduce the leaving bed time and the hospitalization costs, and improve Harris hip joint function score. The incidence of complications such as respiratory system infection, urinary system infection, venous thrombus embolism (VTE), pressure sore, incision infection, constipation, and prosthesis dislocation also has been decreased significantly. Meanwhile, FTS improved patients’ satisfaction apparently. Conclusions This meta-analysis reveals that FTS could significantly shorten the length of stay, alleviate the pain, reduce the leaving bed time and the hospitalization costs, and improve hip function. The incidence of complications also has been decreased significantly. Meanwhile, FTS has been spoken highly in patients in terms of nursing satisfaction. Its efficacy and safety were proved to be reliable.
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- 2021
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32. Identification of Distinct Clinical Phenotypes of Heterogeneous Mechanically Ventilated ICU Patients Using Cluster Analysis
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Xuanhui Chen, Jiaxin Li, Guangjian Liu, Xiujuan Chen, Shuai Huang, Huixian Li, Siyi Liu, Dantong Li, Huan Yang, Haiqing Zheng, Lianting Hu, Lingcong Kong, Huazhang Liu, Abdelouahab Bellou, Liming Lei, and Huiying Liang
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mechanical ventilation ,cluster analysis ,clinical phenotypes ,critical care ,Medicine - Abstract
This retrospective study aimed to derive the clinical phenotypes of ventilated ICU patients to predict the outcomes on the first day of ventilation. Clinical phenotypes were derived from the eICU Collaborative Research Database (eICU) cohort via cluster analysis and were validated in the Medical Information Mart for Intensive Care (MIMIC-IV) cohort. Four clinical phenotypes were identified and compared in the eICU cohort (n = 15,256). Phenotype A (n = 3112) was associated with respiratory disease, had the lowest 28-day mortality (16%), and had a high extubation success rate (~80%). Phenotype B (n = 3335) was correlated with cardiovascular disease, had the second-highest 28-day mortality (28%), and had the lowest extubation success rate (69%). Phenotype C (n = 3868) was correlated with renal dysfunction, had the highest 28-day mortality (28%), and had the second-lowest extubation success rate (74%). Phenotype D (n = 4941) was associated with neurological and traumatic diseases, had the second-lowest 28-day mortality (22%), and had the highest extubation success rate (>80%). These findings were validated in the validation cohort (n = 10,813). Additionally, these phenotypes responded differently to ventilation strategies in terms of duration of treatment, but had no difference in mortality. The four clinical phenotypes unveiled the heterogeneity of ICU patients and helped to predict the 28-day mortality and the extubation success rate.
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- 2023
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33. Dendrobine Alleviates Cellular Senescence and Osteoarthritis via the ROS/NF-κB Axis
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Haitao Chen, Ming Tu, Siyi Liu, Yinxian Wen, and Liaobin Chen
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osteoarthritis ,dendrobine ,chondrocyte senescence ,reactive oxygen species ,NF-κB pathway ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Osteoarthritis (OA) is a degenerative joint disease characterized by low-grade inflammation and cartilage degradation. Dendrobine (DEN) is reported to inhibit inflammation and oxidative stress in some diseases, but its role in chondrocyte senescence and OA progress has not yet been elucidated. Our study aimed to explore the protective effects of DEN on OA both in vitro and in vivo. We found that DEN inhibited extracellular matrix (ECM) degradation and promoted ECM synthesis. Meanwhile, DEN inhibited senescence-associated secretory phenotype (SASP) factors expression and senescence phenotype in IL-1β-treated chondrocytes. Furthermore, DEN improved mitochondrial function and reduced the production of intracellular reactive oxygen species (ROS). Also, DEN suppressed IL-1β-induced activation of the NF-κB pathway. Further, using NAC (ROS inhibitor), we found that DEN might inhibit NF-κB cascades by reducing ROS. Additionally, X-ray, micro-CT, and histological analyses in vivo demonstrated that DEN significantly alleviated cartilage inflammation, ECM degradation, and subchondral alterations in OA progression. In conclusion, DEN inhibits SASP factors expression and senescence phenotype in chondrocytes and alleviated the progression of OA via the ROS/NF-κB axis, which provides innovative strategies for the treatment of OA.
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- 2023
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34. Cellular assays identify barriers impeding iron-sulfur enzyme activity in a non-native prokaryotic host
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Francesca D'Angelo, Elena Fernández-Fueyo, Pierre Simon Garcia, Helena Shomar, Martin Pelosse, Rita Rebelo Manuel, Ferhat Büke, Siyi Liu, Niels van den Broek, Nicolas Duraffourg, Carol de Ram, Martin Pabst, Emmanuelle Bouveret, Simonetta Gribaldo, Béatrice Py, Sandrine Ollagnier de Choudens, Frédéric Barras, and Gregory Bokinsky
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iron-sulfur enzyme ,horizontal gene transfer ,microbial engineering ,electron transfer protein ,Medicine ,Science ,Biology (General) ,QH301-705.5 - Abstract
Iron-sulfur (Fe-S) clusters are ancient and ubiquitous protein cofactors and play irreplaceable roles in many metabolic and regulatory processes. Fe-S clusters are built and distributed to Fe-S enzymes by dedicated protein networks. The core components of these networks are widely conserved and highly versatile. However, Fe-S proteins and enzymes are often inactive outside their native host species. We sought to systematically investigate the compatibility of Fe-S networks with non-native Fe-S enzymes. By using collections of Fe-S enzyme orthologs representative of the entire range of prokaryotic diversity, we uncovered a striking correlation between phylogenetic distance and probability of functional expression. Moreover, coexpression of a heterologous Fe-S biogenesis pathway increases the phylogenetic range of orthologs that can be supported by the foreign host. We also find that Fe-S enzymes that require specific electron carrier proteins are rarely functionally expressed unless their taxon-specific reducing partners are identified and co-expressed. We demonstrate how these principles can be applied to improve the activity of a radical S-adenosyl methionine(rSAM) enzyme from a Streptomyces antibiotic biosynthesis pathway in Escherichia coli. Our results clarify how oxygen sensitivity and incompatibilities with foreign Fe-S and electron transfer networks each impede heterologous activity. In particular, identifying compatible electron transfer proteins and heterologous Fe-S biogenesis pathways may prove essential for engineering functional Fe-S enzyme-dependent pathways.
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- 2022
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35. Volatility Risk Premium, Return Predictability, and ESG Sentiment: Evidence from China’s Spots and Options’ Markets
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Zhaohua Liu, Susheng Wang, Siyi Liu, Haixu Yu, and He Wang
- Subjects
Electronic computers. Computer science ,QA75.5-76.95 - Abstract
This study investigates the volatility risk premium on the emerging financial market. We also consider the expected return and ESG sentiment. Based on the SSE 50 ETF 5-minute high-frequency spots and daily options data from 2016 to 2021, we adopt nonparametric model-free approaches to calculate realized and implied volatilities. And the volatility risk premium is constructed by subtracting these volatility series. We examine the relations between the volatility risk premium and future excess returns as well as ESG sentiment through multifactor specifications. We find that the volatility risk premium also exists in the Chinese market and is significantly negative. In addition, the statistically positive correlation between the volatility risk premium and aggregate returns is an outlier compared to the empirically negative pattern in developed markets. At last, ESG sentiment is positively associated with the volatility risk premium, especially the impact of environmental and social. This evidence supports the agency theory, which indicates that investors perceive ESG investments as waste resources in a short term and become potentially risky.
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- 2022
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36. Effects of dietary sweeteners supplementation on growth performance, serum biochemicals, and jejunal physiological functions of broiler chickens
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Jingle Jiang, Siyi Liu, Tuniyaz Jamal, Tengxin Ding, Lina Qi, Zengpeng Lv, Debing Yu, and Fangxiong Shi
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sweetener ,broiler ,stevioside ,sucralose ,saccharin sodium ,Animal culture ,SF1-1100 - Abstract
The objective of this study was to investigate the effects of dietary 3 kinds of sweeteners supplementation on growth performance, serum biochemicals, and jejunal physiological functions of broiler chickens for 21 D. A total of one hundred ninety-two 1-day-old male Ross 308 broiler chicks were randomly divided into 4 treatments with 6 replicates for each treatment. The treatments were basal diet (CON), a basal diet supplemented with 250 mg/kg stevioside (STE), a basal diet supplemented with 100 mg/kg sucralose (SUC), and a basal diet supplemented with 600 mg/kg saccharin sodium (SAC). All birds were housed in 3-level battery cages. The results showed that dietary STE supplementation increased (P
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- 2020
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37. Reconstruction of a Distal Foot Skin Defect Using an Intermediate Dorsal Neurocutaneous Flap
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Siyi Liu, Jinhai Tan, Shengxiang Tao, Yong Duan, Xiang Hu, and Zonghuan Li
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Cutaneous arteries ,Distal foot defect ,Neurocutaneous flap ,Reconstruction ,Orthopedic surgery ,RD701-811 - Abstract
Objective To present the use of an intermediate dorsal neurocutaneous flap for the reconstruction of defects on the distal foot. Methods From September 2016 to October 2018, five patients (mean age at operation 33.8 years; range, 7–70 years; female/male = 2/3) with skin defects on one of their feet caused by road‐traffic accidents, electrical injury, and syndactyly correction were retrospectively reviewed. The size of the defects ranged from 2.0 cm × 1.0 cm to 5.0 cm × 3.5 cm. All patients had undergone a reconstruction surgery using intermediate dorsal neurocutaneous flap. One patient underwent a syndactyly correction, and four patients first experienced aggressive debridement. The sizes of the flaps were between 5.0 cm × 2.0 cm and 6.0 cm × 4.0 cm. The function, appearance, and pain of the injured foot were assessed using the Chinese Manchester Foot Pain and Disability Index and visual analogue scale. Results These five patients were systematically followed up for a mean of 15.8 months (range, 12–20 months). The donor sites were closed primarily in two cases, and skin grafts were performed in three cases. All the flaps survived with a success rate of 100%; the wounds healed well, and the color matches were excellent. Partial superficial flap necrosis occurred in one of five flaps, which was treated by dressing change using a hypertonic saline gauze. No significant problems were found at the donor site in any patient immediately afterwards or at follow‐up. There were no problems in any patients associated with wearing shoes. Based on the Chinese Manchester Foot Pain and Disability Index, four patients were strongly satisfied and one was satisfied with the recovery of physical function; all the patients were strongly satisfied with the appearance of the injured foot; all five patients had an excellent score of pain intensity subscale. Except for one patient who reported mild pain, all the other patients reported no pain based on the visual analogue scale. Two typical cases are presented in this paper. Conclusions The intermediate dorsal neurocutaneous flap is an alternative and effective technique that can reliably cover minor‐ to medium‐sized defects on the distal foot, toes, and web spaces. This surgical method leads to satisfactory functional recovery with minimal donor site morbidity, and no major vessels need to be sacrificed. This procedure offers an advisable option for orthopaedic surgeons to treat defects on the distal foot.
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- 2020
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38. Serum C-reactive protein to albumin ratio and mortality associated with peritoneal dialysis
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Siyi Liu, Panlin Qiu, Laimin Luo, Lei Jiang, Yanbing Chen, Caixia Yan, and Xiaojiang Zhan
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serum c-reactive protein to albumin ratio ,peritoneal dialysis ,mortality ,serum c-reactive protein ,albumin ,Diseases of the genitourinary system. Urology ,RC870-923 - Abstract
Background Serum C-reactive protein to albumin ratio (CAR) was recently identified as a poor marker of prognosis among various populations. The current study aimed to examine the association between CAR and all-cause mortality among patients undergoing peritoneal dialysis (PD). Methods A total of 758 patients with PD were included in this study during the period from 1 November 2005 to 28 February 2017 and followed up until 31 May 2017. The primary outcome was all-cause mortality. We used multivariate Cox proportional hazard models and Kaplan-Meier survival curves to assess the relationship between CAR and all-cause mortality in these patients. Results Among 758 participants, mean age was 49.1 ± 14.2 years, with 56% males and 18.6% prevalence of diabetes. Median CAR was 0.13 (interquartile range [IQR], 0.07–0.34). After 27 months (IQR, 14–40 months) of follow-up, 157 deaths had been reported. After adjusting for confounding factors, we found a significant association between serum CAR and all-cause mortality among those in the highest CAR group (hazard ratio 1.91, 95% confidence interval 1.05– 3.47, p = 0.034). Conclusions In patients undergoing PD, an increase in serum CAR is independently associated with increased risk for all-cause mortality.
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- 2020
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39. Synthesis and Characterization of an Analgesic Potential Conotoxin Lv32.1
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Siyi Liu, Cheng Li, Shen You, Qinghui Yan, Sulan Luo, and Ying Fu
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analgesic conotoxin ,Lv32.1 ,Conus lividus ,α9α10 nAChR ,Nav1.8 channel ,Organic chemistry ,QD241-441 - Abstract
In our work of screening analgesic peptides from the conotoxin libraries of diverse Conus species, we decoded a peptide sequence from Conus lividus and named it Lv32.1 (LvXXXIIA). The folding conditions of linear Lv32.1 on buffer, oxidizing agent, concentration of GSH/GSSG and reaction time were optimized for a maximum yield of (34.94 ± 0.96)%, providing an efficient solution for the synthesis of Lv32.1. Its disulfide connectivity was identified to be 1–3, 2–6, 4–5, which was first reported for the conotoxins with cysteine framework XXXII and different from the common connectivities established for conotoxins with six cysteines. The analgesic effect of Lv32.1 was determined by a hot plate test in mice. An evident increase in the pain threshold with time illustrated that Lv32.1 exhibited analgesic potency. The effects on Nav1.8 channel and α9α10 nAChR were detected, but weak inhibition was observed. In this work, we highlight the efficient synthesis, novel disulfide linkage and analgesic potential of Lv32.1, which laid a positive foundation for further development of conotoxin Lv32.1 as an analgesic candidate.
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- 2022
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40. Anti-Ovarian Cancer Conotoxins Identified from Conus Venom
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Shuang Ju, Yu Zhang, Xijun Guo, Qinghui Yan, Siyi Liu, Bokai Ma, Mei Zhang, Jiaolin Bao, Sulan Luo, and Ying Fu
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anti-ovarian cancer ,disulfide connectivity ,disulfide-rich conotoxins ,voltage-gated sodium channel ,Conus venom ,Organic chemistry ,QD241-441 - Abstract
Conotoxins constitute a treasury of drug resources and have attracted widespread attention. In order to explore biological candidates from the marine cone snail, we isolated and identified three novel conopeptides named as Vi14b, Vi002, Vi003, three conotoxin variants named as Mr3d.1, Mr3e.1, Tx3a.1, and three known conotoxins (Vi15a, Mr3.8 and TCP) from crude venoms of Conus virgo, Conus marmoreus and Conus texile. Mr3.8 (I-V, II-VI, III-IV) and Tx3a.1 (I-III, II-VI, IV-V) both showed a novel pattern of disulfide connectivity, different from that previously established for the µ- and ψ-conotoxins. Concerning the effect on voltage-gated sodium channels, Mr3e.1, Mr3.8, Tx3a.1, TCP inhibited Nav1.4 or Nav1.8 by 21.51~24.32% of currents at semi-activated state (TP2) at 10 μmol/L. Certain anti-ovarian cancer effects on ID-8 cells were exhibited by Tx3a.1, Mr3e.1 and Vi14b with IC50 values of 24.29 µM, 54.97 µM and 111.6 µM, respectively. This work highlights the role of conotoxin libraries in subsequent drug discovery for ovarian cancer treatment.
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- 2022
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41. Author Correction: Human eye-inspired soft optoelectronic device using high-density MoS2-graphene curved image sensor array
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Changsoon Choi, Moon Kee Choi, Siyi Liu, Minsung Kim, Ok Kyu Park, Changkyun Im, Jaemin Kim, Xiaoliang Qin, Gil Ju Lee, Kyoung Won Cho, Myungbin Kim, Eehyung Joh, Jongha Lee, Donghee Son, Seung-Hae Kwon, Noo Li Jeon, Young Min Song, Nanshu Lu, and Dae-Hyeong Kim
- Subjects
Science - Published
- 2022
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42. A new type of DNA phosphorothioation-based antiviral system in archaea
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Lei Xiong, Siyi Liu, Si Chen, Yao Xiao, Bochen Zhu, Yali Gao, Yujing Zhang, Beibei Chen, Jie Luo, Zixin Deng, Xiangdong Chen, Lianrong Wang, and Shi Chen
- Subjects
Science - Abstract
The prokaryote defence system Dnd relies on phosphorothioation (PT) of DNA backbone to distinguish between self and non-self DNA. Here, Xiong et al. describe a previously uncharacterized PT-based antiviral system that is independent of the canonical Dnd and is widespread in Archaea and Bacteria.
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- 2019
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43. Serum alkaline phosphatase levels correlate with long-term mortality solely in peritoneal dialysis patients with residual renal function
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Xiaojiang Zhan, Yuting Yang, Yanbing Chen, Xin Wei, Jun Xiao, Li Zhang, Caixia Yan, Panlin Qiu, Siyi Liu, Qinglan Hu, Qinkai Chen, and Yu Wang
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alkaline phosphatase ,residual renal function ,long-term mortality ,peritoneal dialysis ,Diseases of the genitourinary system. Urology ,RC870-923 - Abstract
Introduction: Increased serum alkaline phosphatase (ALP) is predictive of a higher mortality in patients with end-stage renal disease. However, it remains unknown whether residual renal function (RRF) influences the outcome-association of serum ALP among peritoneal dialysis (PD) patients. Methods: A total of 650 incident PD patients receiving PD catheter implantation in an institute between 1 November 2005 and 28 February 2017 were retrospectively enrolled. These patients were divided into groups with and without RRF (RRF and non-RRF groups) and those with serum ALP levels in tertiles. The Kaplan–Meier method and multivariate Cox proportional hazard models were used to analyze their outcomes based on RRF and serum ALP levels. Results: These 650 patients had a mean age of 49.4 ± 14.0 years old, their median ALP level was 74 U/L (interquartile range (IQR): 59–98). After 28-month (IQR: 14–41) follow-up, 80 patients in RRF group and 40 patients in non-RRF group died. PD patients with the highest serum ALP tertile had significant lower survival (p = .014), when compared to other patients in the RRF group. However, this relationship was not observed in patients in the non-RRF group. After multivariate adjustment, in the RRF group, patients with the highest ALP tertile had a significantly higher risk of mortality (hazard ratio (HR): 2.26, 95% confidence interval (CI): 1.06–4.82, p = .034). Each 10-U/L increase in ALP level was associated with a 4% (HR: 1.04, 95% CI: 1.00–1.08, p = .045) higher mortality risk. Conclusions: Higher serum ALP level is associated with increased mortality solely in PD patients with RRF.
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- 2019
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44. Perceived Benefits and Forest Tourists Consumption Intention: Environmental Protection Attitude and Resource Utilization Attitude as Mediators
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Bin Zhou, Siyi Liu, Hu Yu, Dongfang Zhu, and Qihao Xiong
- Subjects
forest tourism ,perceived benefits ,consumption intention ,environmental protection attitude ,resource utilization attitude ,Siming Mountain ,Plant ecology ,QK900-989 - Abstract
Establishing a relationship model between environmental protection and resource utilization attitude and consumption intention is the key to promoting the sustainable development of forest tourism. From the standpoint of the Stimulus–Organism–Response (SOR) framework, the purpose of this study is to explore the complex causal relationships between perceived benefits, attitudes toward environmental protection, resource utilization attitudes, and consumption intentions in the context of forest tourism. The research data have been collected using a questionnaire survey of 436 tourists at Siming Mountain in the suburbs of Ningbo city, China. Furthermore, it is analyzed by structural equation modeling. The results indicate a positive correlation between the perceived benefits and tourists’ consumption intention that is mediated by the tourists’ attitude toward resource utilization. Although the independent mediating effect of environmental protection attitude is not supported in this study, both attitudes have played a chain-mediating role between perceived benefit and consumption intention. This study contributes to the existing knowledge by measuring the impact of perceived benefits and environmental attitudes of forest tourists on consumption intentions.
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- 2022
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45. The complete mitochondrial genome of Cimbex luteus (Hymenoptera: Cimbicidae) and phylogenetic analysis
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Yuchen Yan, Ke Li, Siyi Liu, Gengyun Niu, and Meicai Wei
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mitogenome ,gene rearrangement ,phylogeny ,cimbicidae ,cimbex ,Genetics ,QH426-470 - Abstract
The complete mitochondrial genome of Cimbex luteus was sequenced with 15,127 bp in length. The mitogenome includes 13 protein-coding genes, 22 transfer RNAs, two ribosomal RNAs genes, and an AT-rich region. The nucleotide compositions of C. luteus (43.7% A, 38.0% T, 7.4% G, and 10.9% C) were biased toward A and T. Based on Bayesian inference (BI) and Maximum Likelihood (ML) analyses, C. luteus was identified as one of the basal lineages of family Cimbicidae.
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- 2021
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46. Chemical Composition of Plant Residues Regulates Soil Organic Carbon Turnover in Typical Soils with Contrasting Textures in Northeast China Plain
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Siyi Liu, Jiangye Li, Aizhen Liang, Yan Duan, Haibin Chen, Zhuyun Yu, Ruqin Fan, Haiyang Liu, and Hong Pan
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CO2 emission ,crop residues chemistry ,soil textures ,soil organic carbon ,Agriculture - Abstract
Soil organic carbon (SOC) turnover plays a pivotal role in achieving C neutrality, promoting C retention and increasing soil fertility. Residue biochemistry and soil texture essentially determine SOC distribution (including CO2 mineralization and stock in soil) in farmland. However, less is known about allocation of residue-C with contrasting biochemistry and the fate of residue-C in soil under two different textures. This study was conducted in a 61-day aerobic incubation with two Black soils with distinct texture (clay loam vs. sandy loam) in Northeast China. Chemical composition of seven residue parts (soybean roots, leaves, and stems and maize roots, leaves and top and bottom stem parts) was characterized using solid-state 13C nuclear magnetic resonance spectroscopy. The results showed that leaves of both two crops contained significantly higher nitrogen (N), carbonyl and aryl concentrations and lower carbon (C) and lignin concentrations than other parts, resulted in faster decomposition in soils, especially in the clay loam. Stems contained higher O-alkyl and di-O-alkyl concentrations, C/N and lignin/N, while roots contained higher aromaticity. Maize top stem parts with larger slow C pool and longer half-life had higher contribution to SOC accumulation than other parts. Soil textures also induced great impact on SOC turnover. The clay loam favored SOC sequestration due to significantly longer half-life of slow C pool than the sandy loam. Generally, the alkyl/O-alkyl ratio showed the most significant correlation with SOC, CO2 emission and soil biochemical factors in the clay loam; whereas in sandy loam, the lignin/N was the pivotal indicator for SOC accumulation. This study provides insights into the differences in chemical composition among various residue parts, and highlights the significant effects of both residue chemical composition and soil texture on residue decomposition and SOC accumulation.
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- 2022
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47. Research on Reservoir Optimal Operation Based on Long-Term and Mid-Long-Term Nested Models
- Author
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Chongxun Mo, Shutan Zhao, Yuli Ruan, Siyi Liu, Xingbi Lei, Shufeng Lai, Guikai Sun, and Zhenxiang Xing
- Subjects
reservoir optimal operation ,nested model ,dynamic programming ,genetic algorithm ,index evaluation ,Hydraulic engineering ,TC1-978 ,Water supply for domestic and industrial purposes ,TD201-500 - Abstract
In order to solve the problem that the existing optimal operation model of reservoirs cannot coordinate the contradiction between long-term and short-term benefits, the paper nested the long-term optimal operation and mid-long-term optimal operations of reservoirs and established the multi-objective optimal operation nested model of reservoirs. At the same time, based on this model, the optimal control mode is determined when there are errors in the predicted runoff. In the optimal scheduling nested model, the dynamic programming algorithm is used to determine the long-term optimal scheduling solution, and the genetic algorithm is used to solve the mid-long-term optimal scheduling. The optimal control mode is determined by three indicators: power generation benefit, water level over limit risk rate and the not-exploited water volume. The results show that, on the premise of meeting the flood control objectives, the nested model optimal dispatching plan has higher benefits than the long-term optimal dispatching plan and the actual dispatching plan, which verifies the superiority of the nested model in the reservoir optimal dispatching problem. When there is error in predicting runoff, among the water level control mode, flow control mode and output control mode, the average power generation benefit of output control mode is 150.05 GW·h, the low-risk rate of water level overrun is 0.29, and the not-exploited water volume is 39,270 m3. Compared with the water level control mode and the flow control mode, the output control mode has the advantages of higher power generation efficiency, lower water level over limit risk rate and less not-exploited water volume. Therefore, from the perspective of economic benefit and risk balance, the output control mode in the optimization scheduling nested mode is the optimal control mode.
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- 2022
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48. Apolipoprotein B/apolipoprotein A1 ratio and mortality among incident peritoneal dialysis patients
- Author
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Xiaojiang Zhan, Yanbing Chen, Caixia Yan, Siyi Liu, Lijuan Deng, Yuting Yang, Panlin Qiu, Dan Pan, Bingxiang Zeng, and Qinkai Chen
- Subjects
Apolipoprotein ,Cardiovascular event ,Cohort study ,Mortality ,Peritoneal dialysis ,Nutritional diseases. Deficiency diseases ,RC620-627 - Abstract
Abstract Background To investigate the association between the ratio of apolipoprotein B (apo B) / apolipoprotein A1 (apo A1) with all-cause mortality and cardiovascular events in peritoneal dialysis (PD) patients. Methods Eight hundred and sixty incident PD patients were enrolled from November 1, 2005, to February 28, 2017, and followed until May 31, 2017. Outcomes were all-cause mortality and cardiovascular events. Associations between the apo B/apo A1 ratio with all-cause mortality and cardiovascular events were evaluated using multivariable-adjusted Cox models. Results Of the 860 patients, the mean age was 49.9 ± 14.5 years, 57.6% were men, and 19.3% were diabetic patients. The median apo B/apo A1 ratio was 0.65 (range: 0.22–2.24). During a median follow-up period of 27 months (interquartile range, 13 – 41 months), 202 deaths, and 145 cardiovascular events were recorded. After adjustment for age, sex, body mass index, diabetes, cardiovascular disease, systolic blood pressure, total Kt/V, estimated glomerular filtration rate, hemoglobin level, neutrophil to lymphocyte ratio and albumin, triglyceride, and cholesterol, as well as the use of lipid-lowering agents, the highest apo B/apo A1 ratio tertile was significantly associated with a hazard ratio for all-cause mortality of 1.60 (95% CI: 1.02 to 2.49, P = 0.040) and for cardiovascular events of 2.04 (95% CI: 1.21 to 3.44, P = 0.008). Conclusion An increased apo B/apo A1 ratio was independently associated with all-cause mortality and cardiovascular events in PD patients.
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- 2018
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49. Human eye-inspired soft optoelectronic device using high-density MoS2-graphene curved image sensor array
- Author
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Changsoon Choi, Moon Kee Choi, Siyi Liu, Min Sung Kim, Ok Kyu Park, Changkyun Im, Jaemin Kim, Xiaoliang Qin, Gil Ju Lee, Kyoung Won Cho, Myungbin Kim, Eehyung Joh, Jongha Lee, Donghee Son, Seung-Hae Kwon, Noo Li Jeon, Young Min Song, Nanshu Lu, and Dae-Hyeong Kim
- Subjects
Science - Abstract
Soft and flexible optoelectronic devices may provide effective routes toward retinal implants for enhanced visual functions. Here, the authors fabricate a curved array of flexible MoS2-graphene photodetectors and demonstrate its potential application as ophthalmic imaging element in mouse models.
- Published
- 2017
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50. Mode identification of low‐frequency oscillations in power systems based on fourth‐order mixed mean cumulant and improved TLS‐ESPRIT algorithm
- Author
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Tao Jin, Siyi Liu, and Rodolfo C.C. Flesch
- Subjects
low‐frequency oscillation mode identification ,power systems ,TLS‐ESPRIT algorithm ,wide area monitoring systems ,WAMS ,Gaussian white noise ,Distribution or transmission of electric power ,TK3001-3521 ,Production of electric energy or power. Powerplants. Central stations ,TK1001-1841 - Abstract
Wide area monitoring systems (WAMS) provide effective support for online identification of low‐frequency oscillations in power systems. The WAMS signal is sensitive to the surrounding environment, and it contains Gaussian white noise. The Gaussian white noise will produce Gaussian coloured noise through the filter, which will bring some errors to the mode identification. In order to solve the above problem, this study proposes a method to identify the low‐frequency oscillation modes of a single‐channel measurement of the power system based on the combination of the fourth‐order mixed mean cumulant and the improved TLS‐ESPRIT (total least square‐estimation parameter space rotation invariant technique). The actual signal is replaced by the processed signal using the fourth‐order mixed mean cumulant mathematical statistical method, thereby the Gaussian coloured noise is suppressed effectively; then, TLS‐ESPRIT algorithm is used to identify the signal, which is used to identify the low‐frequency oscillation of each mode. A novel relative change rate of singular value was also introduced in order to avoid the artificially threshold‐setting error. The simulation results show that the proposed method has better anti‐noise performance and higher accuracy of fitting than other methods reported in literature, besides being easy to be implemented and suitable for performing on‐line identification.
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- 2017
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