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7. Plant hemoglobin gene expression adjusts Arabidopsis susceptibility to Pseudomonas synringae and Botrytis cinerea though scavenging of nitric oxide

Author

Sivakumaran, Anushen, Hebelstrup, Kim, Cristescu, Simona, Harren, Frans, Hall, Michael A, and Mur, Luis A J

Abstract

NO has earlier been shown to influence ethylene production during Pseudomonas syringae elicited hypersensitive response in tobacco. In this work Arabidopsis plants with silencing or null mutation of hemoglobin genes (glb1 and glb2) and transgenic lines over-expressing Glb1 and Glb2 demonstrated a causal link between NO generation, hemoglobin-dependent NO scavenging, the production of ethylene and resistance to Botrytis or Pseudomonas. NO has earlier been shown to influence ethylene production during Pseudomonas syringae elicited hypersensitive response in tobacco. In this work Arabidopsis plants with silencing or null mutation of hemoglobin genes (glb1 and glb2) and transgenic lines over-expressing Glb1 and Glb2 demonstrated a causal link between NO generation, hemoglobin-dependent NO scavenging, the production of ethylene and resistance to Botrytis or Pseudomonas.

Published
2011

9. ABA Suppresses Botrytis cinerea Elicited NO Production in Tomato to Influence H2O2 Generation and Increase Host Susceptibility.

Author

Sivakumaran, Anushen, Akinyemi, Aderemi, Mandon, Julian, Cristescu, Simona M., Hall, Michael A., Harren, Frans J. M., Mur, Luis A. J., Staal, Jens, and Kachroo, Aardra

Subjects
ABSCISIC acid, ETHYLENE compounds, NITRIC-oxide synthases
Abstract

Abscisic acid (ABA) production has emerged a susceptibility factor in plant-pathogen interactions. This work examined the interaction of ABA with nitric oxide (NO) in tomato following challenge with the ABA-synthesizing pathogen, Botrytis cinerea. Trace gas detection using a quantum cascade laser detected NO production within minutes of challenge with B. cinerea whilst photoacoustic laser detection detected ethylene production - an established mediator of defense against this pathogen - occurring after 6 h. Application of the NO generation inhibitor N-Nitro-L-arginine methyl ester (L-NAME) suppressed both NO and ethylene production and resistance against B. cinerea. The tomato mutant sitiens fails to accumulate ABA, shows increased resistance to B. cinerea and we noted exhibited elevated NO and ethylene production. Exogenous application of L-NAME or ABA reduced NO production in sitiens and reduced resistance to B. cinerea. Increased resistance to B. cinerea in sitiens have previously been linked to increased reactive oxygen species (ROS) generation but this was reduced in both L-NAME and ABA-treated sitiens. Taken together, our data suggests that ABA can decreases resistance to B. cinerea via reduction of NO production which also suppresses both ROS and ethylene production. [ABSTRACT FROM AUTHOR]

Published
2016
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