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227 results on '"Siu, L. Kristopher"'

11. A 20-Year Study of Capsular Polysaccharide Seroepidemiology, Susceptibility Profiles, and Virulence Determinants of Klebsiella pneumoniae from Bacteremia Patients in Taiwan

Author

Tsai, Chun-Chou, primary, Lin, Jung-Chung, additional, Chen, Pei-Chen, additional, Liu, Esther Yip-Mei, additional, Tsai, Yu-Kuo, additional, Yu, Chia-Peng, additional, Li, Jia-Je, additional, Wang, Ching-Hsun, additional, Fung, Chang-Phone, additional, Lin, Fu-Mei, additional, Chang, Feng-Yee, additional, and Siu, L. Kristopher, additional

Published
2023
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14. Placental mesenchymal stem cells boost M2 alveolar over M1 bone marrow macrophages via IL-1β in Klebsiella-mediated acute respiratory distress syndrome.

Author

Li-Tzu Wang, Yen, B. Linju, Hsiu-Huan Wang, Ying-Yin Chao, Wei Lee, Li-Yueh Huang, Sheng-Kang Chiu, Siu, L. Kristopher, Ko-Jiunn Liu, Huey-Kang Sytwu, and Men-Luh Yen

Subjects
ADULT respiratory distress syndrome, MESENCHYMAL stem cells, KLEBSIELLA pneumoniae, MEDICAL sciences, BONE marrow, MACROPHAGES, PULMONARY fibrosis, ABRUPTIO placentae
Published
2023
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18. Placental mesenchymal stem cells boost M2 alveolar over M1 bone marrow macrophages via IL-1β in Klebsiella-mediated acute respiratory distress syndrome

Author

Wang, Li-Tzu, primary, Yen, B Linju, additional, Wang, Hsiu-Huan, additional, Chao, Ying-Yin, additional, Lee, Wei, additional, Huang, Li-Yueh, additional, Chiu, Sheng-Kang, additional, Siu, L. Kristopher, additional, Liu, Ko-Jiunn, additional, Sytwu, Huey-Kang, additional, and Yen, Men-Luh, additional

Published
2022
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24. Klebsiella pneumoniae in gastrointestinal tract and pyogenic liver abscess

Author

Fung, Chang-Phone, Lin, Yi-Tsung, Lin, Jung-Chung, Chen, Te-Li, Yeh, Kuo-Ming, Chang, Feng-Yee, Chuang, Han-Chuan, Wu, Hau-Shin, Tseng, Chih-Peng, and Siu, L. Kristopher

Subjects
Abdomen -- Abscess, Liver diseases -- Causes of -- Diagnosis -- Research, Klebsiella -- Identification and classification -- Research, Health
Abstract

Klebsiella pneumoniae has emerged as the predominant cause of pyogenic liver abscess in Taiwan and other countries in eastern and Southeast Asia (1-4). This condition is frequently associated with severe [...]

Published
2012
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26. Additional file 1 of Virulence among different types of hypervirulent Klebsiella pneumoniae with multi-locus sequence type (MLST)-11, Serotype K1 or K2 strains

Author

Wang, Tsui-Chin, Lin, Jung-Chung, Chang, Jen-Chang, Hiaso, Ya-Wen, Wang, Ching-Hsun, Chiu, Sheng‑Kung, Fung, Chang-Phone, Chang, Feng-Yee, and Siu, L. Kristopher

Abstract

Additional file 1: Tabls S1. PCR primer sets for virulence gene detection and serotyping. Table S2. Primers used for in-frame deletion of iucA and wza.

Published
2021
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28. Placental mesenchymal stem cells boost M2 alveolar over M1 bone marrow macrophages via IL-1β in Klebsiella-mediated acute respiratory distress syndrome

Author

Wang, Li-Tzu, Yen, B Linju, Wang, Hsiu-Huan, Chao, Ying-Yin, Lee, Wei, Huang, Li-Yueh, Chiu, Sheng-Kang, Siu, L. Kristopher, Liu, Ko-Jiunn, Sytwu, Huey-Kang, and Yen, Men-Luh

Abstract

RationaleAcute respiratory distress syndrome (ARDS) is a lethal complication of severe bacterial pneumonia due to the inability to dampen overexuberant immune responses without compromising pathogen clearance. Both of these processes involve tissue-resident and bone marrow (BM)–recruited macrophage (MΦ) populations which can be polarised to have divergent functions. Surprisingly, despite the known immunomodulatory properties of mesenchymal stem cells (MSCs), simultaneous interactions with tissue-resident and recruited BMMΦ populations are largely unexplored.ObjectivesWe assessed the therapeutic use of human placental MSCs (PMSCs) in severe bacterial pneumonia with elucidation of the roles of resident alveolar MΦs (AMΦs) and BMMΦs.MethodsWe developed a lethal, murine pneumonia model using intratracheal infection of a clinically relevant Klebsiella pneumoniae(KP) strain with subsequent intravenous human PMSC treatment. Pulmonary AMΦ and recruited BMMΦ analyses, histological evaluation, bacterial clearance and mice survival were assessed. To elucidate the role of resident AMΦs in improving outcome, we performed AMΦ depletion in the KP-pneumonia model with intratracheal clodronate pretreatment.Measurements and main resultsHuman PMSC treatment decreased tissue injury and improved survival of severe KP-pneumonia mice by decreasing the presence and function of recruited M1 BMMΦ while preserving M2 AMΦs and enhancing their antibacterial functions. Interestingly, PMSC therapy failed to rescue AMΦ-depleted mice with KP pneumonia, and PMSC-secreted IL-1β was identified as critical in increasing AMΦ antibacterial activities to significantly improve pathogen clearance—especially bacteraemia—and survival.ConclusionsHuman PMSC treatment preferentially rescued resident M2 AMΦs over recruited M1 BMMΦs with overall M2 polarisation to improve KP-related ARDS survival.

Published
2023
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31. Does Antimicrobial Therapy Affect Mortality of Patients with Carbapenem-Resistant Klebsiella pneumoniae Bacteriuria? A Nationwide Multicenter Study in Taiwan

Author

Chuang, Chien, primary, Su, Chin-Fang, additional, Lin, Jung-Chung, additional, Lu, Po-Liang, additional, Huang, Ching-Tai, additional, Wang, Jann-Tay, additional, Chuang, Yin-Ching, additional, Siu, L. Kristopher, additional, Fung, Chang-Phone, additional, and Lin, Yi-Tsung, additional

Published
2020
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34. Truncated Pneumolysin from Streptococcus pneumoniae as a TLR4-Antagonizing New Drug for Chronic Inflammatory Conditions

Author

Chang, Shun-Fu, primary, Chen, Cheng-Nan, additional, Lin, Jung-Chung, additional, Wang, Hsin-Ell, additional, Mori, Shigetarou, additional, Li, Jia-Je, additional, Yen, Chia-Kuang, additional, Hsu, Ching-Yun, additional, Fung, Chang-Phone, additional, Chong, Pele Choi-Sing, additional, Leng, Chih-Hsiang, additional, Ding, Yi-Jun, additional, Chang, Feng-Yee, additional, and Siu, L. Kristopher, additional

Published
2020
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35. Resistance mechanisms and molecular epidemiology of carbapenem-nonsusceptible Escherichia coli in Taiwan, 2012-2015

Author

Chang,Ya-Ting, Siu,L. Kristopher, Wang,Jann-Tay, Wu,Tsu-Lan, Chen,Yu-Hui, Chuang,Yin-Ching, Lin,Jung-Chung, and Lu,Po-Liang

Subjects
Infection and Drug Resistance, biochemical phenomena, metabolism, and nutrition
Abstract

Ya-Ting Chang,1 L Kristopher Siu,2 Jann-Tay Wang,3 Tsu-Lan Wu,4 Yu-Hui Chen,5,6Yin-Ching Chuang,7,8Jung-Chung Lin,9 Po-Liang Lu1,101Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan; 2National Institutes of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli, Taiwan; 3Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan; 4Department of Clinical Pathology, Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan; 5Infection Control Center, Chi Mei Medical Center, Tainan, Taiwan; 6Department of Nursing, College of Medicine and Life Science, Chung Hwa University of Medical Technology, Tainan, Taiwan; 7Department of Internal Medicine and Medical Research, Chi Mei Medical Center, Tainan, Taiwan; 8Department of Internal Medicine, Chi Mei Medical Center, Tainan, Taiwan; 9Division of Infectious Diseases and Tropical Medicine, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan; 10College of Medicine, Kaohsiung Medical University, Kaohsiung, TaiwanPurpose: This study aimed to investigate the resistance mechanisms and molecular epidemiology of carbapenem-nonsusceptible Escherichia coli (CnsEC) in Taiwan.Patients and methods: From 2012 to 2015, 237 E. coli isolates with minimum inhibitory concentrations of imipenem or meropenem >1μg/mL were collected in a nationwide surveillance and subjected to polymerase chain reaction (PCR) for carbapenemase, AmpC-type β-lactamase, and extended spectrum β-lactamase (ESBL) genes. We evaluated outer membrane proteins (OmpF and OmpC) loss and conducted multilocus sequence typing and pulsed-field gel electrophoresis (PFGE). Isolates that were resistant to all carbapenems were designated as pan-carbapenem-resistant E. coli (pCREC) in this study.Results: The predominant resistance mechanism of CnsEC in Taiwan was the CMY-2 β-lactamase in combination with OmpF and OmpC loss. Sequence type 131 was the most prevalent type (29.2%). Among 237 CnsEC isolates, 106 (44.7%) isolates were pCREC and 18 (7.59%) produced carbapenemase. The prevalence of carbapenemases increased from 6% in 2012 to 11.36% in 2015. Various carbapenemases including KPC-2, IMP-8, NDM-1, NDM-5, VIM-1, OXA-48, and OXA-181 were identified, with NDM-1 being the most common (38.9%) carbapenemase. Comparison between pCREC and non-pCREC among the non-carbapenemase-producing CnsEC isolates revealed SHV, CMY, co-carriage of SHV and CTX-M and concurrent loss of both OmpF and OmpC were more commonly detected in the pCREC group. PFGE revealed no nationwide clonal spread of carbapenemase-producing E. coli.Conclusion: NDM-1 was the most common carbapenemase and combination of CMY-2 and concurrent OmpF and OmpC porin loss was the most prevalent resistance mechanism in CnsEC in Taiwan.Keywords: multidrug resistance, carbapenemase, Enterobacteriaceae, epidemiology

Published
2019

36. Seroepidemiology of Klebsiella pneumoniae colonizing the intestinal tract of healthy chinese and overseas chinese adults in Asian countries

Author

Lin Yi-Tsung, Siu L Kristopher, Lin Jung-Chung, Chen Te-Li, Tseng Chih-Peng, Yeh Kuo-Ming, Chang Feng-Yee, and Fung Chang-Phone

Subjects
Microbiology, QR1-502
Abstract

Abstract Background Capsular serotypes K1 and K2 of Klebsiella pneumoniae are thought to the major virulence determinants responsible for liver abscess. The intestine is one of the major reservoirs of K. pneumoniae, and epidemiological studies have suggested that the majority of K. pneumoniae infections are preceded by colonization of the gastrointestinal tract. The possibility of fecal-oral transmission in liver abscess has been raised on the basis of molecular typing of isolates. Data on the serotype distribution of K. pneumoniae in stool samples from healthy individuals has not been previously reported. This study investigated the seroepidemiology of K. pneumoniae isolates from the intestinal tract of healthy Chinese in Asian countries. Stool specimens from healthy adult Chinese residents of Taiwan, Japan, Hong Kong, China, Thailand, Malaysia, Singapore, and Vietnam were collected from August 2004 to August 2010 for analysis. Results Serotypes K1/K2 accounted for 9.8% of all K. pneumoniae isolates from stools in all countries. There was no significant difference in the prevalence of K1/K2 isolates among the countries excluding Thailand and Vietnam. The antimicrobial susceptibility pattern was nearly the same in K. pneumoniae isolates. The result of pulsed-field gel electrophoresis revealed no major clonal cluster of serotype K1 isolates. Conclusions The result showed that Chinese ethnicity itself might be a major factor predisposing to intestinal colonization by serotype K1/K2 K. pneumoniae isolates. The prevalent serotype K1/K2 isolates may partially correspond to the prevalence of K. pneumoniae liver abscess in Asian countries.

Published
2012
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37. Genotypes and virulence in serotype K2 Klebsiella pneumoniae from liver abscess and non-infectious carriers in Hong Kong, Singapore and Taiwan

Author

Lin, Jung-Chung, Koh, Tse Hsien, Lee, Nelson, Fung, Chang-Phone, Chang, Feng-Yee, Tsai, Yu-Kuo, Ip, Margaret, and Siu, L Kristopher

Subjects
Health
Abstract

In Klebsiella pneumoniae liver abscess (KP-LA), K. pneumonia e K2 is the most frequently isolated serotype after K1, but this serotype has been much less studied. In the present study, the molecular types sequences type (MLST) of serotype K2 isolates from three different regions in Asia were identified and the virulence of these isolates was investigated. Eight different MLSTs were found among 26 isolates (ST 65, 66, 86, 373, 374, 375, 380, and 434). There were two major MLST groups, ST-65-like (42%) and ST86-like (46%). No isolates contained allS while all isolates contained rmpA. The prevalence of aerobactin gene and kfu were 25/26 (96%) and 3/26 (11.5%) respectively. Although liver abscess isolates were generally more resistant (11/15 isolates) to serum killing, there was no specific distribution of serum killing resistant or susceptible ST types between stool carriage and liver abscess isolates. Neutrophil phagocytosis showed that the liver abscess and carriage isolates varied in their susceptibility to phagocytosis. Strains with resistance to both neutrophil phagocytosis and serum killing were generally hypervirulent with lethality at LD.sub.50 < 10.sup.3 colony forming units by intraperitoneal injection. In conclusion, Anti-phagocytosis and resistance to serum killing are two parameters that most predict hyperviurlence in serotype K2 isolates. Unlike serotype K1 KP-LA that mainly belong to ST-23, ST-65-like and -86-like are the two major MLST types among serotype K2 isolates from Singapore, Hong Kong and Taiwan. Keywords: Liver abscess, Klebsiella pneumoniae, MLST, Author(s): Jung-Chung Lin[sup.1] , Tse Hsien Koh[sup.2] , Nelson Lee[sup.3] , Chang-Phone Fung[sup.4] , Feng-Yee Chang[sup.1] , Yu-Kuo Tsai[sup.5] , Margaret Ip[sup.6] and L Kristopher Siu[sup.1,5,7] Introduction Klebsiella pneumoniae liver [...]

Published
2014
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39. Appropriate Treatment for Bloodstream Infections Due to Carbapenem-Resistant Klebsiella pneumoniae and Escherichia coli: A Nationwide Multicenter Study in Taiwan

Author

Lin, Yi-Tsung, primary, Su, Chin-Fang, additional, Chuang, Chien, additional, Lin, Jung-Chung, additional, Lu, Po-Liang, additional, Huang, Ching-Tai, additional, Wang, Jann-Tay, additional, Chuang, Yin-Ching, additional, Siu, L Kristopher, additional, and Fung, Chang-Phone, additional

Published
2018
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42. Treatment outcome of non-carbapenemase-producing carbapenem-resistant Klebsiella pneumoniae infections: a multicenter study in Taiwan

Author

Su, Chin-Fang, primary, Chuang, Chien, additional, Lin, Yi-Tsung, additional, Chan, Yu-Jiun, additional, Lin, Jung-Chung, additional, Lu, Po-Liang, additional, Huang, Ching-Tai, additional, Wang, Jann-Tay, additional, Chuang, Yin-Ching, additional, Siu, L. Kristopher, additional, and Fung, Chang-Phone, additional

Published
2017
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