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2. Actin-based motility of Burkholderia thailandensis requires a central acidic domain of BimA that recruits and activates the cellular Arp2/3 complex.

Author

Sitthidet C, Stevens JM, Field TR, Layton AN, Korbsrisate S, and Stevens MP

Subjects
Bacterial Proteins genetics, Burkholderia genetics, Electrophoresis, Polyacrylamide Gel, Microfilament Proteins genetics, Microscopy, Confocal, Actins metabolism, Bacterial Proteins metabolism, Burkholderia metabolism, Microfilament Proteins metabolism
Abstract

Burkholderia species use BimA for intracellular actin-based motility. Uniquely, Burkholderia thailandensis BimA harbors a central and acidic (CA) domain. The CA domain was required for actin-based motility, binding to the cellular Arp2/3 complex, and Arp2/3-dependent polymerization of actin monomers. Our data reveal distinct strategies for actin-based motility among Burkholderia species.

Published
2010
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3. Prevalence and sequence diversity of a factor required for actin-based motility in natural populations of Burkholderia species.

Author

Sitthidet C, Stevens JM, Chantratita N, Currie BJ, Peacock SJ, Korbsrisate S, and Stevens MP

Subjects
Amino Acid Sequence, Animals, Burkholderia mallei genetics, Burkholderia pseudomallei isolation & purification, DNA, Bacterial genetics, Endemic Diseases, Humans, Melioidosis epidemiology, Melioidosis microbiology, Molecular Sequence Data, Sequence Alignment, Sequence Analysis, DNA, Burkholderia pseudomallei genetics, Locomotion, Microfilament Proteins genetics, Polymorphism, Genetic
Abstract

Actin-based motility of the melioidosis pathogen Burkholderia pseudomallei requires BimA. We report a high degree of conservation of bimA in 99 B. pseudomallei isolates from the area of endemicity. A geographically restricted subset of B. pseudomallei isolates harbored a B. mallei-like bimA allele (12.1%), confounding a differential diagnostic test based on amplification of species-specific bimA regions.

Published
2008
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