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3. Unveiling prey preferences of endangered wild Malayan tiger, Panthera tigris jacksoni, in Peninsular Malaysia through scat analysis via COI DNA metabarcoding

Author

Gani, Millawati, primary, Sitam, Frankie Thomas, additional, Kamarudin, Zubaidah, additional, Selamat, Siti Suzana, additional, Awang, Nik Mohd Zamani, additional, Muhd-Sahimi, Hani Nabilia, additional, Wong, Michael, additional, Selat, Baharim, additional, Abdullah-Halim, Nur Fatin Khairunnisa, additional, Yong, Lim Shu, additional, Yoke, Ling Fong, additional, Yaakop, Salmah, additional, Mohd-Ridwan, Abd Rahman, additional, and Md-Zain, Badrul Munir, additional

Published
2024
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5. The Society for Wildlife Forensic Science standards and guidelines

Author

Moore, M. Katherine, Baker, Barry W., Bauman, Tasha L., Burnham-Curtis, Mary K., Espinoza, Edgard O., Ferrell, Carolyn S., Frankham, Greta J., Frazier, Kim, Giles, Jenny L., Hawk, Deedra, Rovie-Ryan, Jeffrine J., Johnson, Rebecca N., Knott, Trey, Kornfield, Irving L., Lindquist, Christina, Lord, Wayne D., Morgan, Kelly L., O’Brien, R. Christopher, Ogden, Rob, Prigge, Tracey-Leigh, Schwenke, Piper, Sitam, Frankie T., Trail, Pepper, Wictum, Elizabeth, Wilson, Paul J., Yates, Bonnie C., and Webster, Lucy M.I.

Published
2021
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8. Phylogeography of the Sunda pangolin, Manis javanica: Implications for taxonomy, conservation management and wildlife forensics

Author

Sitam, Frankie T., primary, Salgado‐Lynn, Milena, additional, Denel, Azroie, additional, Panjang, Elisa, additional, McEwing, Ross, additional, Lightson, Amanda, additional, Ogden, Rob, additional, Maruji, Nur Alwanie, additional, Yahya, Nurhartini Kamalia, additional, Ngau, Cosmas, additional, Mohd Kulaimi, Noor Azleen, additional, Ithnin, Hartini, additional, Rovie‐Ryan, Jeffrine, additional, Abu Bakar, Mohd Soffian, additional, and Ewart, Kyle M., additional

Published
2023
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9. Unveiling prey preferences of endangered wild Malayan tiger, Panthera tigris jacksoni, in Peninsular Malaysia through scat analysis via COI DNA metabarcoding.

Author

Gani, Millawati, Sitam, Frankie Thomas, Kamarudin, Zubaidah, Selamat, Siti Suzana, Awang, Nik Mohd Zamani, Muhd-Sahimi, Hani Nabilia, Wong, Michael, Selat, Baharim, Abdullah-Halim, Nur Fatin Khairunnisa, Yong, Lim Shu, Yoke, Ling Fong, Yaakop, Salmah, Mohd-Ridwan, Abd Rahman, and Md-Zain, Badrul Munir

Subjects
TIGERS, CYTOCHROME oxidase, GENETIC barcoding, CANIDAE, DNA, NUTRITIONAL requirements
Abstract

Understanding the prey preference of Malayan tiger (Panthera tigris jacksoni) in Malaysia is important to guide conservation planning initiatives. The utilisation of DNA metabarcoding provides valuable insights, particularly in the field of carnivora diet research. This technique has been proven to be effective for identifying various species within complex mixtures such as scat materials, where visual identification is challenging. The Cytochrome c oxidase subunit I (COI) locus has been selected as it is a widely used as an effective non-invasive approach for diet studies. Hence, given this advance approach, Malayan tiger scats were collected on the basis of existing records of their presence in two types of habitats, namely, protected areas (PA) and human–tiger conflict (HTC) areas. This study aimed to identify prey species in Peninsular Malaysia, based on Malayan tiger scat samples using DNA metabarcoding. Based on the partial mitochondrial COI region, DNA metabarcoding led to the taxonomic resolution of prey DNA remnants in scats and the identification of prey species consumed by Malayan tiger, which were predominately small-to-medium-sized prey, including livestock. The dominant DNA prey detected belongs to the family Canidae, followed by Bovidae, Vespertilionidae, Homonidae, Felidae, Phasianidae and Muridae. A significant difference (p < 0.05) was observed in alpha and beta diversity using the Shannon index and PERMANOVA with regard to prey richness and evenness in two different habitat groups, namely, PA and HTC. Our finding provides insights into Malayan tiger dietary requirements, which can be used to develop conservation plans and strategies for Malayan tiger, particularly for habitat priorities. [ABSTRACT FROM AUTHOR]

Published
2024
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10. Phylogeography of the Sunda pangolin, Manis javanica: implications for taxonomy, conservation management and wildlife forensics

Author

Sitam, Frankie, primary, Salgado-Lynn, Milena, additional, Denel, Azroie, additional, Panjang, Elisa, additional, McEwing, Ross, additional, Lightson, Amanda, additional, Ogden, Rob, additional, Maruji, Nur Alwanie, additional, Yahya, Nurhartini Kamalia, additional, Ngau, Cosmas, additional, Kulaimi, Noor Azleen Mohd, additional, Ithnin, Hartini, additional, Rovie-Ryan, Jeffrine, additional, Bakar, Mohd Soffian Abu, additional, and Ewart, Kyle, additional

Published
2023
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11. The complete mitochondrial genome of Red Junglefowl (Gallus gallus spadiceus) from Peninsular Malaysia

Author

Muhd Nazmi Amir Mazlan, Mohd-Kulaimi, Noor Azleen, Sitam, Frankie Thomas, Norsyamimi Rosli, Ayub, Qasim, Yong, Lim Shu, and Badrul Munir Md-Zain

Subjects
Mitogenome, Gallus gallus spadiceus, next-generation sequencing, red junglefowl
Abstract

Complete mitochondrial genome sequences have significant relevance in the study of phylogenetic relationships, evolution, and population genetics. In this paper, we present the complete mitochondrial genome of the red junglefowl (Gallus gallus spadiceus) from Peninsular Malaysia, inferred using next-generation sequencing (NGS). The mitogenome is 16,785 bp in length with the structural organization of an avian mitochondrial arrangement compromising 13 protein-coding regions, 22 tRNAs, 2 rRNAs, and 1 control region. No internal stop codon was found in the protein-coding genes. Overall base composition is A: 30.3%, C: 32.5%, G: 13.5%, and T: 23.7%, indicating a high A + T content of 54.0%. Phylogenetic tree analysis revealed that red junglefowl from Peninsular Malaysia is grouped together with other members of Gallus gallus specifically from Southeast Asia, with 89% bootstrap value support. These research findings might be beneficial for red junglefowl genetic identification, molecular systematic studies, and conservation management interest in the future.

Published
2023
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12. Little Evidence of Zika Virus Infection in Wild Long-Tailed Macaques, Peninsular Malaysia

Author

Chua, Chong Long, Chan, Yoke Fun, Andu, Eva S.G. Soh, Rovie-Ryan, Jeffrine J., Sitam, Frankie Thomas, Verasahib, Khebir, and Sam, I-Ching

Subjects
Yellow fever -- Analysis, Primates -- Analysis, Dengue virus -- Analysis, Zika virus -- Analysis, Disease transmission -- Analysis, Antibodies -- Analysis, Mosquitoes, Genetic disorders, RNA, Health
Abstract

Zika virus, first isolated from a rhesus macaque (Macaca mulatta) in the Zika Forest in Uganda, reemerged in the Pacific Islands and Americas in 2015 and caused unprecedented outbreaks associated [...]

Published
2019

13. Genome sequence analysis of Malayan pangolin (Manis javanica) forensic samples reveals the presence of Paraburkholderia fungorum sequences.

Author

Ka Yun Tan, Siwei Deng, Tze King Tan, Hari, Ranjeev, Sitam, Frankie Thomas, Othman, Rofina Yasmin, Kum Thong Wong, Mohd Mohidin, Taznim Begam, and Siew Woh Choo

Subjects
SEQUENCE analysis, STAINS & staining (Microscopy), FETAL tissues, SKELETAL muscle, BACTERIAL DNA, BACTERIAL genomes, NUCLEOTIDE sequencing
Abstract

Background: The Malayan pangolin (Manis javanica) is a placental mammal and is listed as Critically Endangered on the IUCN Red List of Threatened Species. Most previous attempts to breed pangolins in captivity have met with little success because of dietary issues, infections, and other complications, although a previous study reported breeding pangolins in captivity to the third generation. In our previous pangolin genome sequencing data analysis, we obtained a considerable amount of bacterial DNA from a pregnant female Malayan pangolin (named "UM3"), which was likely infected by Paraburkholderia fungorum--an agent of biodegradation and bioremediation in agriculture. Methodology: Here, we further confirmed and characterized this bacterial species using PCR, histological staining, whole-genome sequencing, and bioinformatics approaches. PCR assays with in-house designed primer sets and 16S universal primers showed clear positive bands in the cerebrum, cerebellum, lung, and blood of UM3 suggesting that UM3 might have developed septicaemia. Histological staining showed the presence of Gram-negative rod-shaped bacteria in the pangolin brain and lungs, indicating the colonization of the bacteria in these two organs. In addition, PCR screening of UM3's fetal tissues revealed the presence of P. fungorum in the gastrocnemius muscle, but not in other tissues that we examined. We also sequenced and reconstructed the genome of pangolin P. fungorum, which has a genome size of 7.7 Mbps. Conclusion: Our study is the first to present detailed evidence of the presence of P. fungorum in a pangolin and her fetus (although preliminary results were presented in our previous article). Here, we raise the concern that P. fungorum may potentially infect humans, especially YOPI (young, old, pregnant, and immunocompromised) people. Therefore, caution should be exercised when using this bacterial species as biodegradation or bioremediation agents in agriculture. [ABSTRACT FROM AUTHOR]

Published
2023
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14. Complete mitochondrial genome of Red Junglefowl (Gallus gallus spadiceus) from Peninsular Malaysia.

Author

Amir Mazlan, Muhd Nazmi, Mohd-Kulaimi, Noor Azleen, Sitam, Frankie Thomas, Rosli, Norsyamimi, Ayub, Qasim, Lim Shu Yong, and Md-Zain, Badrul Munir

Subjects
CHICKENS, RIBOSOMAL RNA, MITOCHONDRIAL physiology, TRANSFER RNA
Abstract

Complete mitochondrial genome sequences have significant relevance in the study of phylogenetic relationships, evolution, and population genetics. In this paper, we present the complete mitochondrial genome of the red junglefowl (Gallus gallus spadiceus) from Peninsular Malaysia, inferred using next-generation sequencing (NGS). The mitogenome is 16,785 bp in length with the structural organization of an avian mitochondrial arrangement compromising 13 proteincoding regions, 22 tRNAs, 2 rRNAs, and 1 control region. No internal stop codon was found in the protein-coding genes. Overall base composition is A: 30.3%, C: 32.5%, G: 13.5%, and T: 23.7%, indicating a high A + T content of 54.0%. Phylogenetic tree analysis revealed that red junglefowl from Peninsular Malaysia is grouped together with other members of Gallus gallus specifically from Southeast Asia, with 89% bootstrap value support. These research findings might be beneficial for red junglefowl genetic identification, molecular systematic studies, and conservation management interest in the future. [ABSTRACT FROM AUTHOR]

Published
2023
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15. Macacine herpesvirus 1 in long-tailed Macaques, Malaysia, 2009-2011

Author

Lee, Mei-Ho, Rostal, Melinda K., Hughes, Tom, Sitam, Frankie, Lee, Ohee-Yen, Japning, Jeffrine, Harden, Mallory E., Griffiths, Anthony, Basir, Misliah, Wolfe, Nathan D., Epstein, Jonathan H., and Daszak, Peter

Subjects
Disease transmission -- Forecasts and trends, Macaques -- Diseases, Herpesvirus infections -- Diagnosis, Medical research, Health
Abstract

Macacine herpesvirus 1 (MaHVI; also known as B virus) is a zoonotic pathogen that is enzootic among macaque (Macaca spp.) populations throughout Asia (1,2). MaHVI is an [alpha]-herpesvirus related to [...]

Published
2015
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16. Figure 3 from: Gani M, Rovie-Ryan JJ, Sitam FT, Kulaimi NAM, Zheng CC, Atiqah AN, Rahim NMA, Mohammed AA (2021) Taxonomic and genetic assessment of captive White-Handed Gibbons (Hylobates lar) in Peninsular Malaysia with implications towards conservation translocation and reintroduction programmes. ZooKeys 1076: 25-41. https://doi.org/10.3897/zookeys.1076.73262

Author

Gani, Millawati, primary, Rovie-Ryan, Jeffrine J., additional, Sitam, Frankie Thomas, additional, Kulaimi, Noor Azleen Mohd, additional, Zheng, Chew Cheah, additional, Atiqah, Aida Nur, additional, Rahim, Nur Maisarah Abd, additional, and Mohammed, Ahmad Azhar, additional

Published
2021
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17. Figure 2 from: Gani M, Rovie-Ryan JJ, Sitam FT, Kulaimi NAM, Zheng CC, Atiqah AN, Rahim NMA, Mohammed AA (2021) Taxonomic and genetic assessment of captive White-Handed Gibbons (Hylobates lar) in Peninsular Malaysia with implications towards conservation translocation and reintroduction programmes. ZooKeys 1076: 25-41. https://doi.org/10.3897/zookeys.1076.73262

Author

Gani, Millawati, primary, Rovie-Ryan, Jeffrine J., additional, Sitam, Frankie Thomas, additional, Kulaimi, Noor Azleen Mohd, additional, Zheng, Chew Cheah, additional, Atiqah, Aida Nur, additional, Rahim, Nur Maisarah Abd, additional, and Mohammed, Ahmad Azhar, additional

Published
2021
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18. Figure 1 from: Gani M, Rovie-Ryan JJ, Sitam FT, Kulaimi NAM, Zheng CC, Atiqah AN, Rahim NMA, Mohammed AA (2021) Taxonomic and genetic assessment of captive White-Handed Gibbons (Hylobates lar) in Peninsular Malaysia with implications towards conservation translocation and reintroduction programmes. ZooKeys 1076: 25-41. https://doi.org/10.3897/zookeys.1076.73262

Author

Gani, Millawati, primary, Rovie-Ryan, Jeffrine J., additional, Sitam, Frankie Thomas, additional, Kulaimi, Noor Azleen Mohd, additional, Zheng, Chew Cheah, additional, Atiqah, Aida Nur, additional, Rahim, Nur Maisarah Abd, additional, and Mohammed, Ahmad Azhar, additional

Published
2021
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19. Supplementary material 3 from: Gani M, Rovie-Ryan JJ, Sitam FT, Kulaimi NAM, Zheng CC, Atiqah AN, Rahim NMA, Mohammed AA (2021) Taxonomic and genetic assessment of captive White-Handed Gibbons (Hylobates lar) in Peninsular Malaysia with implications towards conservation translocation and reintroduction programmes. ZooKeys 1076: 25-41. https://doi.org/10.3897/zookeys.1076.73262

Author

Gani, Millawati, primary, Rovie-Ryan, Jeffrine J., additional, Sitam, Frankie Thomas, additional, Kulaimi, Noor Azleen Mohd, additional, Zheng, Chew Cheah, additional, Atiqah, Aida Nur, additional, Rahim, Nur Maisarah Abd, additional, and Mohammed, Ahmad Azhar, additional

Published
2021
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20. Taxonomic and genetic assessment of captive White-Handed Gibbons (Hylobates lar) in Peninsular Malaysia with implications towards conservation translocation and reintroduction programmes

Author

Gani, Millawati, primary, Rovie-Ryan, Jeffrine J., additional, Sitam, Frankie Thomas, additional, Kulaimi, Noor Azleen Mohd, additional, Zheng, Chew Cheah, additional, Atiqah, Aida Nur, additional, Rahim, Nur Maisarah Abd, additional, and Mohammed, Ahmad Azhar, additional

Published
2021
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21. Supplementary material 2 from: Gani M, Rovie-Ryan JJ, Sitam FT, Kulaimi NAM, Zheng CC, Atiqah AN, Rahim NMA, Mohammed AA (2021) Taxonomic and genetic assessment of captive White-Handed Gibbons (Hylobates lar) in Peninsular Malaysia with implications towards conservation translocation and reintroduction programmes. ZooKeys 1076: 25-41. https://doi.org/10.3897/zookeys.1076.73262

Author

Gani, Millawati, primary, Rovie-Ryan, Jeffrine J., additional, Sitam, Frankie Thomas, additional, Kulaimi, Noor Azleen Mohd, additional, Zheng, Chew Cheah, additional, Atiqah, Aida Nur, additional, Rahim, Nur Maisarah Abd, additional, and Mohammed, Ahmad Azhar, additional

Published
2021
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22. Supplementary material 1 from: Gani M, Rovie-Ryan JJ, Sitam FT, Kulaimi NAM, Zheng CC, Atiqah AN, Rahim NMA, Mohammed AA (2021) Taxonomic and genetic assessment of captive White-Handed Gibbons (Hylobates lar) in Peninsular Malaysia with implications towards conservation translocation and reintroduction programmes. ZooKeys 1076: 25-41. https://doi.org/10.3897/zookeys.1076.73262

Author

Gani, Millawati, primary, Rovie-Ryan, Jeffrine J., additional, Sitam, Frankie Thomas, additional, Kulaimi, Noor Azleen Mohd, additional, Zheng, Chew Cheah, additional, Atiqah, Aida Nur, additional, Rahim, Nur Maisarah Abd, additional, and Mohammed, Ahmad Azhar, additional

Published
2021
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24. Socializing One Health: an innovative strategy to investigate social and behavioral risks of emerging viral threats

Author

Saylors, Karen, Wolking, David J., Hagan, Emily, Martinez, Stephanie, Francisco, Leilani, Euren, Jason, Olson, Sarah H., Miller, Maureen, Fine, Amanda E., Thanh, Nga Nguyen Thi, Tran Minh, Phuc, Kalengkongan, Jusuf D., Kusumaningrum, Tina, Latinne, Alice, Pamungkas, Joko, Safari, Dodi, Saputro, Suryo, Bamba, Djeneba, Coulibaly, Kalpy Julien, Dosso, Mireille, Laudisoit, Anne, N'guettia Jean, Kouassi Manzan, Dutta, Shusmita, Islam, Ariful, Shano, Shahanaj, Mwanzalila, Mwokozi I., Trupin, Ian P., Gbakima, Aiah, Bangura, James, Yondah, Sylvester T., Karmacharya, Dibesh, Shrestha, Rima D., Kamta, Marcelle Annie Matsida, Mouiche, Mohamed Moctar Mouliom, Ndolo, Hilarion Moukala, Niama, Fabien Roch, Onikrotin, Dionne, Daszak, Peter, Johnson, Christine K., Mazet, Jonna A. K., Abaneh, Ola, Ababneh, Mustafa, Rafia, Jum, Sukor, Abd, Abdullah, Mohd Lufti, Abedin, Josefina, Abu-Basha, Ehab, Ali, Mohamed, Beal Akoundze, Junior, Akpaki, Joel, Al Hanandeh, Sief Addeen, Al Omari, Bilal, Al Shakil, Abdullah, Al-Zghoul, Mohammed, Albart, Stephenie Ann, Alshammari, Abdullah, Amarneh, Basil H., Ampofo, William, Andrew, Victoria, Ahn, Dao Le, Ankhanbaatar, Ulaankhuu, Anthony, Simon, Antonjaya, Ungke, Araya, Kidan, Arku, Jallah, Arshat, Norsharina, Asigbee, Theodore, Aung, Ohnmar, Awuni, Joseph, Ayukebong, James, Azian, Mohammed, Aziz, Nor Adilah, Ba, Aminata, Bassan, Ganzorig, Bagato, Ola, Bamba, Aboubacar, Bamba, Djenba, Barkhasbaatar, Ariunbaatar, Barrera, June, Basaraba, Cale, Bel-nono, Samuel, Belaganahalli, Manjunatha, Belay, Desalgen, Belkharia, Jaber, Binol, Ridzki M. F., Bird, Brian, Bista, Manisha, Biswas, Pitu, Blake, Matthew, Boatemaa, Linda, Bonason, Margret, Brandful, James, Brown, Joseph, Brownstein, John, Camara, Mamadi, Camara, Salif, Chai, Daniel, Chakraborty, Debapriyo, Chale, Hannah, Chaudhary, Ashok, Chea, Sokha, Chmura, Aleksei, Goossens, Benoit, Chow, Andrew, Churchill, Carolina, Commey, Abraham, Couacy-Hymann, Emmanuel, Coulibaly, Julien Kalpy, Cranfield, Michael, Damanik, Wirda, Damdinjav, Batchullum, Danial, Norhidayah, Dasak, Peter, David, Runie, Dawson, Patrick, Dembele, Arjouma, Deme, Awa, Desmond, James, Dewantari, Arghianditya Kresno, Dhanota, Jaseet, Dhole, Tapan, Diep, Nguyen Thi, Dionkounda, Artistide, Diop, Gaye Laye, Dodd, Kimberly, Dogby, Otilia, Dorjnyam, Tumendemberel, Dosso, Mireielle, Doumbouya, Kalil, Doumbouya, Mohamed Idriss, Doyle, Megan, Dramou, Simone, Drazenovich, Tracy, Duc Ahn, Dang, Duc Luu, Bach, Duendkae, Prateep, Duoc, Vu Trong, Duong, Tran Nhu, Duong, Veasna, Dursman, Huda, Dussart, Phillipe, Ee, Tan Jun, Ekiri, Abel, El Rifay, Amira S., El Shesheny, Rabel, El Taween, Ahmed N., Emmanuel, Zena Babu, Epstein, Jonathan H., Evans, Tierra Smiley, Fahmawi, Alaa, Fahn, Simeon, Feferholtz, Yasha, Ferdous, Jinnat, Fine, Amanda, Flora, Meerjady, Fransisco, Leilani, Fui Fui, Lem, Gabourie, Taylor, Gani, Millawati, Garbo, Michael, Gardner, Nicole, Gbamele, Marie, Ge, Xingyi, Gee, Lee Heng, Genovese, Brooke, Gibson, Alexandra, Gilardi, Kirsten, Gilbert, Martin, Gillis, Amethyst, Ginsos, Andrew, Godji Gnabro, Privat, Goldstein, Tracey, Gomaa, Moktar, Gomis, Jules, Gonzalez, Kevin, Grange, Zoe, Greig, Denise, Grodus, Michael, Gueu, Kpon Kakeuma Romeo, Gutierrez, Leticia, Haba, Dan Marcelin, Hamid, Suraya, Harris, Daniel K., Hashim, Abdul Kadir Abu, Hassan, Moushumi, Hassan, Quazar Nizamuddin, He, Qun, Hemachudha, Thiravat, Henry, Helen, Herbert, Ronald, Hijazeen, Zaidoun, Hilarion, Moukala Ndolo, Hill, Rebecca, Hoa, Nguyen Thi, Horwood, Paul, Hossain, Md. Enayet, Hossain, Saddam, Htun, Moh Moh, Hu, Ben, Hughes, Tom, Hul, Vibol, Van, Vo, Hussein, Fatima, Indola, Ghislain Dzeret, Iskandriati, Diah, Islam, Md. Tarikul, Islam, Shariful, Isnaim Ismail, Mohd, Ismail, Zuhair Bani, Iyanya, Jacques, Jaimin, Joel Judson, Jambai, Amara, Japning, Jeffrine Rovie Ryan, Japrin, Alexter, Jean Louis, Frantz, Joe, Titus, Johnson, Erica, Joly, Damien, Joshi, Jyotsna, Kalengkongan, Jusuf, Kalivogui, Douokoro, Kamara-Chieyoe, Nenneh, Kamau, Joseph, Kambale Syaluha, Eddy, Kandeil, Ahmed, Kane, Yogouba, Karesh, William, Kargo, Kandeh, Kasenda, Novie, Kayali, Ghazi, Kayed, Ahmed S., Kazwala, Rudovick, Ke, Changwen, Keates, Lucy, Kebede, Nigatu, Khamphaphongphane, Bouaphanh, Kheong, Chong Chee, Kilonzo, Christopher, Koffa, Ma-Sue, Kollie, Amos G., Kondiano, Marcel Sidik, Koropo, Michel, Kouamé Kouakou, Valere, Kouassi Koffi, Eugene, Kourouma, Mariam, Koutate, Abdoulaye Ousmane, Kowel, Citra Liv, Krou, Hermann Assemien, Kumakamba, Charles, Kutkat, Omnia, Lamah, François, Lan, Nguyen Thi, Lane, Jennifer, Lange, Christian, Larmouth, Emmanuel, Le Doux, Joseph Diffo, Leasure, Elizabeth, Leasure, Katherine, LeBreton, Mat, Lee, Jimmy, Lee, Helen, Lee, Mei Ho, Leno, Amara, Li, Hongying, Liang, Eliza, Liang, Neal, Lim, Dorothy, Lipkin, W. Ian, Liu, Jun, Lo, Modou Moustafa, Lojivis, Leonoris, Long, Nguyen Van, Lucas, Ashley, Lukusa, Jean Paul, Lungay, Victor, Lushima, Shongo, Lutwama, Julius, Ma, Wenjun, Machalaba, Catherine, Maganga, Grace, Magesa, Walter Simon, Mahmoud, Sara H., Makuwa, Maria, Makweta, Asha, Mamun, Abdullah Al, Manandhar, Prajwol, Maneeorn, Patarapol, Mann, Harjeet, Maomy, Bhele, Maptue, Victorine, Mathew, Alice, Mavoungou, Yanne Vanessa, Maw, Min Thein, Mazet, Jonna, Mbala, Placide, Mbuba, Emmanuel, Mbunwe, Eric, McIver, David, Mendelsohn, Emma, Miegakanda, Valchy Bel-Bebi, Minh, Phan Quang, Mkali, Happy, Moatasim, Yassmin, Mombouli, Jean Vivien, Monagin, Corina, Montecino-Latorre, Diego, Mossoun Mossoun, Arsene, Mostafa, Ahmed, Mouiche, Moctar, Mpassi, Romain Bagamboula, Msigwa, Alphonce, Mudakikwa, Antoine, Mugok, Laura Benedict, Mulembakani, Prime, Murray, Suzan, Musa, Fakhrul Hatta, Musabimana, Pacifique, Mutura, Samson, Mwamlima, Tunu, Mwanzanilla, Mwokozi, Myaing, Tin Tin, Myat, Theingi Win, Myo Chit, Aung, N’faly, Magassouba, N’Guettia, Manzan Jean, N’télo, Anatole, Nakimera, Sylivia, Nam, Vu Sinh, Napit, Rajindra, Nathan, Senthilvel K. S. S., Navarrete-Macias, Isamara, Ndebe, Kortu M., Ndiaye, Amadou, Ndiaye, Daouda, Negash, Yohannes, Nga, Nguyen Thi Thanh, Ngay, Ipos, Ngoc, Pham Thi Bich, Niama, Fabien, Nina, Rock Aimé, Niyonzima, Schadrack, Nkom, Felix, Nkoua, Cynthia, Noordin, Noorliza, Noviana, Rachmitasari, Nwobegahay, Julius, Nziza, Julius, O’Rourke, Daniel, O’Rourke, Tammie, Obodai, Evangeline, Okello Okwir, Ricky, Olival, Kevin, Olson, Sarah, Olva, Onkirotin Dionne, Ontiveros, Victoria, Opook, Fernandes, Panchadcharam, Chandrawathani, Pandit, Pranav, Parra, Henri-Joseph, Phuc, Tran Minh, Phuong, Nguyen Thanh, Poultolnor, Jackson Y., Pradhan, Saman, Preston, Eunah Cho, Pruvot, Mathieu, Purevtseren, Dulam, Puri, Dhiraj, Quang, Le Tin Vinh, Rachmitasari, Novie, Rahman, Kaisar, Rahman, Mizanur, Rahman, Mohammed Ziaur, Rahman, Mustafizur, Ramirez, Diana, Randhawa, Nistara, Raut, Samita, Rosario, Joseph, Ross, Albert, Ross, Noam, Rostal, Melinda, Roualdes, Pamela, Rubin, Eddy, Rumi, Aftab Uddin, Rundi, Christina, Sackie, Melkor, Sajali, Zikankuba, Samuels, Sandra G, Sango, Mathias, Saptu, Ammar Rafidah, Saraka, Daniel N’guessan, Sartee, Alvis A., Sayandouno, Sia Alida, Seck, Mame Cheikh, Sedor, Victoria, Sharma, Ajay Narayan, Sharminie, Velsri, Shehata, Mahmoud M., Sheikh, Gafur, Shi, Zhengli, Shiilegdamba, Enkhtuvshin, Shrestha, Bishwo, Shrestha, Rima, Sidibey, Mohammed, Silithammavong, Soubanh, Simon, Daniel, Sion, Emilly, Sipangkui, Symphorosa, Sitam, Frankie Thomas, Smith, Brett, Smith, Bridgette, Smith, Woutrina, Sodnom, Batsikhan, Ssebide, Benard, Suleiman, Maria, Sullivan, Ava, Sungif, Nur Amirah, Suu-Ire, Richard, Sy, Mouhamed, Takuo, Jean Michel, Talafha, Hani, Tamoufe, Ubald, Tetteh, Emmanuel, Than Toe, Aung, Thanda, Lanash, Thanh Long, Ngo, Thein, Wai Zin, Theppangna, Watthana, Thinh, Nguyen Duc, Thuy, Hoang Bich, Thuy, Nguyen Thu, Togami, Eri, Tolno, Moise Bendoua, Tolovou, Kevin, Topani, Rahmat, Tremeau-Bravard, Alexandre, Trupin, Ian, Tumushime, Jean Claude, Tun, Kyaw Yan Naing, Turay, Joseph, Uddin, Helal, Uhart, Marcela, Ureda, Nicole, Valitutto, Marc, Verasahib, Khebir, Vodzak, Megan, Wacharapluesadee, Supaporn, Wahad, Mohammad Yuery Wazlan Abdul, Watson, Brooke, Wells, Heather, White, Allison, Willoughby, Anna, Wiyatno, Ageng, Wolking, David, Yang, Xinglou, Yao, Lim Ming, Yombouno, Sayon, Young, Cristin, Zambrana-Torrelio, Carlos, Zeid, Zahidah Izzati, Zghoul, Ghadeer, Zhang, Libiao, Zhang, Yunzhi, Zhu, Guangjian, Zimmerman, Dawn, Zoumarou, Daba, Aguirre, Alonso, Aguirre, Luis, Akongo, Mark-Joel, Alandia Robles, Erika, Ambu, Laurentius, Ayala Aguilar, Glenda, Barcena, Luis, Barradas, Rosario, Basir, Misliah Mohamad, Bogich, Tiffany, Bounga, Gerard, Buchy, Philippe, Bunn, David, Byaruba, Denis, Cameron, Ken, Carroll, Dennis, Cavero, Nancy, Cespedes, Manuel, Che, Xiaoyu, Chiu, Charles, Chor, Kimashalen, Clements, Andrew, Dary Acevedo, Luz, de Almeida Campos, Angelica, De La Puente, Micaela, de Lamballerie, Xavier, de Paula, Catia, Delwart, Eric, Diffo Le Doux, Joseph, Doyle-Capitman, Catherine, Durigon, Edison, Fair, Joseph, Ferrer-Paris, José R., Formenty, Pierre, Galarza, Isabel, Garcia, Joel, Grard, Gilda, Greatorex, Zoe, Harris, Laurie, Hitchens, Peta, Ho, Mei, Hosseini, Parviez, In, Samath, Iñíguez, Volga, Jain, Komal, Jamaluddin, Abd. Aziz, Johnson, Christine, Jones, Kate, Joyner, Priscilla, Kaba, Serge, Kambale, Eddy, Kataregga, Abdulhameed, Kelly, Terra, Khammavong, Kongsy, Kilpatrick, A. Marm, Laimun, Samsir, Lee, Mei-Ho, LeRoy, Eric, Levinson, Jordan, Levy, Marc, Limachi, Rolando, Loh, Elizabeth, Lowenstine, Linda J., Luis Mollericona, José, Maganga, Ruth, Malakalinga, Joseph, Manhas, Melissa, Marra, Pete, Mbabazi, Rachael, Medellín, Rodrigo, Mendoza, Patricia, Miller, Sireeda, Miranda, Flavia, Mitchell, Megan, Mohamed, Ramlan, Mollard, Debbie, Morse, Stephen, Mouellet, Wivine, Moya, Isabel, Murillo, Yovanna, Murray, Kris, Muyembe Tamfum, Jean-Jacques, Nassar, Fernando, Nathan, Sen, Nsengimana, Olivier, Ogg Keatts, Lucy, Ojeda-Flore, Rafael, Okwir Okello, Ricky, Ondzie, Alain, Paweska, Janusz, Pereira, Alisa, Pereira, Victoria, Perez, Alberto, Perez, Jocelyn, Phon, Simorn, Reed, Patricia, Rejmanek, Dan, Rico, Oscar, Rivera, Rosario, Romero, Monica, Roy, Celina, Saepuloh, Uus, Schneider, Brad, Schwind, Jessica, Singhalath, Sinpakhome, Smith, Kristine, Suárez, Fabiola, Suzan, Gerardo, Thanh Nga, Nguyen Thi, Thomas, Kate, Ticona, Herminio, VanWormer, Elizabeth, Villar, Sandra, Weisman, Wendy, Westfall, Michael, Whittier, Chris, Wicker, Leanne, Wolfe, Nathan, Yang, Angela, Zariquiey, Carlos, Zhang, Shu-Yi, Zorine Nkouants, Baudelaire, Zainuddin, Zainal, Chrisman, Cara, Pabst, August, Shek, Amalhin, and Trostle, Murray

Subjects
0301 basic medicine, Behavioral risk, medicine.medical_specialty, Behavioural sciences, Disease, Community integration, Basic Behavioral and Social Science, 03 medical and health sciences, 0302 clinical medicine, Clinical Research, 2.3 Psychological, Behavioral and Social Science, Agency (sociology), medicine, 2.2 Factors relating to the physical environment, GE1-350, Aetiology, One health, Social science research, business.industry, Prevention, Research, Public health, PREDICT Consortium, Public relations, Focus group, Environmental sciences, Good Health and Well Being, 030104 developmental biology, One Health, Multi-disciplinary surveillance, social and economic factors, Public aspects of medicine, RA1-1270, Infection, Psychology, International development, business, 030217 neurology & neurosurgery
Abstract

In an effort to strengthen global capacity to prevent, detect, and control infectious diseases in animals and people, the United States Agency for International Development’s (USAID) Emerging Pandemic Threats (EPT) PREDICT project funded development of regional, national, and local One Health capacities for early disease detection, rapid response, disease control, and risk reduction. From the outset, the EPT approach was inclusive of social science research methods designed to understand the contexts and behaviors of communities living and working at human-animal-environment interfaces considered high-risk for virus emergence. Using qualitative and quantitative approaches, PREDICT behavioral research aimed to identify and assess a range of socio-cultural behaviors that could be influential in zoonotic disease emergence, amplification, and transmission. This broad approach to behavioral risk characterization enabled us to identify and characterize human activities that could be linked to the transmission dynamics of new and emerging viruses. This paper provides a discussion of implementation of a social science approach within a zoonotic surveillance framework. We conducted in-depth ethnographic interviews and focus groups to better understand the individual- and community-level knowledge, attitudes, and practices that potentially put participants at risk for zoonotic disease transmission from the animals they live and work with, across 6 interface domains. When we asked highly-exposed individuals (ie. bushmeat hunters, wildlife or guano farmers) about the risk they perceived in their occupational activities, most did not perceive it to be risky, whether because it was normalized by years (or generations) of doing such an activity, or due to lack of information about potential risks. Integrating the social sciences allows investigations of the specific human activities that are hypothesized to drive disease emergence, amplification, and transmission, in order to better substantiate behavioral disease drivers, along with the social dimensions of infection and transmission dynamics. Understanding these dynamics is critical to achieving health security--the protection from threats to health-- which requires investments in both collective and individual health security. Involving behavioral sciences into zoonotic disease surveillance allowed us to push toward fuller community integration and engagement and toward dialogue and implementation of recommendations for disease prevention and improved health security. Supplementary Information The online version contains supplementary material available at 10.1186/s42522-021-00036-9.

Published
2021

25. Chikungunya virus in macaques, Malaysia

Author

Sam, I-Ching, Chua, Chong Long, Rovie-Ryan, Jeffrine J., Fu, Jolene Y.L., Tong, Charmaine, Sitam, Frankie Thomas, and Chan, Yoke Fun

Subjects
Health
Abstract

To the Editor: In the past 10 years, chikungunya virus (CHIKV) has caused global epidemics of fever, rash, and arthralgia affecting millions of humans, most recently in the Americas (1). [...]

Published
2015

26. Rickettsial infections in monkeys, Malaysia

Author

Tay, Sun Tee, Koh, Fui Xian, Kho, Kai Ling, and Sitam, Frankie Thomas

Subjects
Rhesus monkey -- Diseases and pests -- Research, Rickettsial diseases -- Risk factors -- Genetic aspects -- Distribution -- Research, Company distribution practices, Health
Abstract

To the Editor: The cynomolgus monkey (Macaca fascicularis), also known as the long-tailed macaque or crabeating monkey, is commonly found in the Southeast Asia region (1). The macaque has been [...]

Published
2015

29. No evidence of coronaviruses or other potentially zoonotic viruses in Sunda pangolins (Manis javanica) entering the wildlife trade via Malaysia

Author

Lee, Jimmy, primary, Hughes, Tom, additional, Lee, Mei-Ho, additional, Field, Hume, additional, Rovie-Ryan, Jeffrine Japning, additional, Sitam, Frankie Thomas, additional, Sipangkui, Symphorosa, additional, Nathan, Senthilvel K.S.S., additional, Ramirez, Diana, additional, Kumar, Subbiah Vijay, additional, Lasimbang, Helen, additional, Epstein, Jonathan H., additional, and Daszak, Peter, additional

Published
2020
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31. Loxodonta Localizer: A Software Tool for Inferring the Provenance of African Elephants and Their Ivory Using Mitochondrial DNA

Author

Zhao, Kai, primary, Ishida, Yasuko, additional, Green, Cory E, additional, Davidson, Alexis G, additional, Sitam, Frankie A T, additional, Donnelly, Cassidy L, additional, De Flamingh, Alida, additional, Perrin-Stowe, Tolulope I N, additional, Bourgeois, Stéphanie, additional, Brandt, Adam L, additional, Mundis, Stephanie J, additional, Van Aarde, Rudi J, additional, Greenberg, Jonathan A, additional, Malhi, Ripan S, additional, Georgiadis, Nicholas J, additional, Mcewing, Ross, additional, and Roca, Alfred L, additional

Published
2019
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32. Taxonomic and genetic assessment of captive White-Handed Gibbons (Hylobates lar) in Peninsular Malaysia with implications towards conservation translocation and reintroduction programmes.

Author

Gani, Millawati, Rovie-Ryan, Jeffrine J., Sitam, Frankie Thomas, Kulaimi, Noor Azleen Mohd, Chew Cheah Zheng, Atiqah, Aida Nur, Rahim, Nur Maisarah Abd, and Mohammed, Ahmad Azhar

Subjects
BAYESIAN field theory, MITOCHONDRIAL DNA, KRA, SEQUENCE analysis, SUBSPECIES
Abstract

Conservation translocation and reintroduction for the purpose of repopulating and reinforcing extirpated or depleted populations has been recognised as an important conservation tool, particularly for gibbon conservation in the immediate future. Feasibility assessments involving multiple factors, including taxonomic and genetic assessment of rescued and captive gibbons, are imperative prior to translocation and reintroduction programmes. In this study, we attempt to determine the subspecies and origin of captive Hylobates lar, White-handed gibbons, from Peninsular Malaysia to assist in future translocation and reintroduction programmes. A total of 12 captive and rescued H. lar samples were analysed using the control region segment of mitochondrial DNA. Sequence analyses and phylogenetic trees constructed using neighbour-joining, maximum likelihood, Bayesian inference, and network methods congruently differentiate all 12 captive individuals used in this study from other H. lar subspecies suggesting that these individuals belong to the H. lar lar subspecies. In addition, two populations of H. l. lar were observed: (1) a southern population consisting of all 12 individuals from Peninsular Malaysia, and (2) a possible northern population represented by three individuals (from previous studies), which might have originated from the region between the Isthmus of Kra, Surat Thani-Krabi depression, and Kangar-Pattani. Our findings suggest that the complete control region segment can be used to determine the subspecies and origin of captive H. lar. [ABSTRACT FROM AUTHOR]

Published
2021
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33. The complete mitochondrial genome of Malayan Gaur (Bos gaurus hubbacki) from Peninsular Malaysia

Author

Rosli, Norsyamimi, Sitam, Frankie Thomas, Rovie-Ryan, Jeffrine Japning, Gan, Han Ming, Lee, Yin Peng, Ithnin, Hartini, Gani, Millawati, Abdul Razak, Mohd Firdaus Ariff, Md-Zain, Badrul Munir, Abdullah, Mohd Tajuddin, Rosli, Norsyamimi, Sitam, Frankie Thomas, Rovie-Ryan, Jeffrine Japning, Gan, Han Ming, Lee, Yin Peng, Ithnin, Hartini, Gani, Millawati, Abdul Razak, Mohd Firdaus Ariff, Md-Zain, Badrul Munir, and Abdullah, Mohd Tajuddin

Published
2019

38. Phylogeographic Evidence for 2 Genetically Distinct Zoonotic Plasmodium knowlesi Parasites, Malaysia.

Author

Yusof, Ruhani, Ahmed, Md Atique, Jelip, Jenarun, Hie Ung Ngian, Mustakim, Sahlawati, Hussin, Hani Mat, Mun Yik Fong, Mahmud, Rohela, Thomas Sitam, Frankie Anak, Japning, J. Rovie-Ryan, Snounou, Georges, Escalante, Ananias A., Yee Ling Lau, Ngian, Hie Ung, Fong, Mun Yik, Sitam, Frankie Anak Thomas, and Lau, Yee Ling

Subjects
PLASMODIUM, MALARIA, PROTOZOAN diseases, NESTED clade analysis, CYTOCHROME oxidase, METALLOENZYMES
Abstract

Infections of humans with the zoonotic simian malaria parasite Plasmodium knowlesi occur throughout Southeast Asia, although most cases have occurred in Malaysia, where P. knowlesi is now the dominant malaria species. This apparently skewed distribution prompted an investigation of the phylogeography of this parasite in 2 geographically separated regions of Malaysia, Peninsular Malaysia and Malaysian Borneo. We investigated samples collected from humans and macaques in these regions. Haplotype network analyses of sequences from 2 P. knowlesi genes, type A small subunit ribosomal 18S RNA and cytochrome c oxidase subunit I, showed 2 genetically distinct divergent clusters, 1 from each of the 2 regions of Malaysia. We propose that these parasites represent 2 distinct P. knowlesi types that independently became zoonotic. These types would have evolved after the sea-level rise at the end of the last ice age, which separated Malaysian Borneo from Peninsular Malaysia. [ABSTRACT FROM AUTHOR]

Published
2016
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39. De novo sequencing, assembly and analysis of eight different transcriptomes from the Malayan pangolin

Author

Mohamed Yusoff, Aini, primary, Tan, Tze King, additional, Hari, Ranjeev, additional, Koepfli, Klaus-Peter, additional, Wee, Wei Yee, additional, Antunes, Agostinho, additional, Sitam, Frankie Thomas, additional, Rovie-Ryan, Jeffrine Japning, additional, Karuppannan, Kayal Vizi, additional, Wong, Guat Jah, additional, Lipovich, Leonard, additional, Warren, Wesley C., additional, O’Brien, Stephen J., additional, and Choo, Siew Woh, additional

Published
2016
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40. Pangolin genomes and the evolution of mammalian scales and immunity

Author

Choo, Siew Woh, primary, Rayko, Mike, additional, Tan, Tze King, additional, Hari, Ranjeev, additional, Komissarov, Aleksey, additional, Wee, Wei Yee, additional, Yurchenko, Andrey A., additional, Kliver, Sergey, additional, Tamazian, Gaik, additional, Antunes, Agostinho, additional, Wilson, Richard K., additional, Warren, Wesley C., additional, Koepfli, Klaus-Peter, additional, Minx, Patrick, additional, Krasheninnikova, Ksenia, additional, Kotze, Antoinette, additional, Dalton, Desire L., additional, Vermaak, Elaine, additional, Paterson, Ian C., additional, Dobrynin, Pavel, additional, Sitam, Frankie Thomas, additional, Rovie-Ryan, Jeffrine J., additional, Johnson, Warren E., additional, Yusoff, Aini Mohamed, additional, Luo, Shu-Jin, additional, Karuppannan, Kayal Vizi, additional, Fang, Gang, additional, Zheng, Deyou, additional, Gerstein, Mark B., additional, Lipovich, Leonard, additional, O'Brien, Stephen J., additional, and Wong, Guat Jah, additional

Published
2016
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42. Phylogeographic Evidence for 2 Genetically Distinct ZoonoticPlasmodium knowlesiParasites, Malaysia

Author

Yusof, Ruhani, primary, Ahmed, Md Atique, additional, Jelip, Jenarun, additional, Ngian, Hie Ung, additional, Mustakim, Sahlawati, additional, Hussin, Hani Mat, additional, Fong, Mun Yik, additional, Mahmud, Rohela, additional, Sitam, Frankie Anak Thomas, additional, Japning, J. Rovie-Ryan, additional, Snounou, Georges, additional, Escalante, Ananias A., additional, and Lau, Yee Ling, additional

Published
2016
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46. Little Evidence of Zika Virus Infection in Wild Long-Tailed Macaques, Peninsular Malaysia.

Author

Chong Long Chua, Yoke Fun Chan, Soh Andu, Eva S. G., Rovie-Ryan, Jeffrine J., Sitam, Frankie Thomas, Verasahib, Khebir, I.-Ching Sam, Chua, Chong Long, Chan, Yoke Fun, Andu, Eva S G Soh, Verasahib, Khebir 1st, Sam, Ching, and Sam, I Ching

Subjects
ZIKA virus infections, KRA
Abstract

We tested a sample of 234 wild long-tailed macaques (Macaca fascicularis) trapped in Peninsular Malaysia in 2009, 2010, and 2016 for Zika virus RNA and antibodies. None were positive for RNA, and only 1.3% were seropositive for neutralizing antibodies. Long-tailed macaques are unlikely to be reservoirs for Zika virus in Malaysia. [ABSTRACT FROM AUTHOR]

Published
2019
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47. Y-chromosomal gene flow of Macaca fascicularis (Cercopithecidae) between the insular and mainland peninsula of Penang state, Malaysia

Author

Japning, Jeffrine Rovie Ryan, Abdullah, Mohd Tajuddin, Sitam, Frankie Thomas, Zainol Abidin, Zaaba, Tan, Soon Guan, Japning, Jeffrine Rovie Ryan, Abdullah, Mohd Tajuddin, Sitam, Frankie Thomas, Zainol Abidin, Zaaba, and Tan, Soon Guan

Abstract

DNA sequencing of the Y-chromosome testis-specific protein locus (TSPY) and sex-determining region (SRY) was conducted on 27 Macaca fascicularis individuals from eight insular and two peninsula localities of Penang State, Malaysia. Five haplotypes were discovered from the Penang samples, of which four were unique for that population. These haplotypes constituted of related individuals as observed in the Y-chromosomal gene flow within and between the insular and mainland regions. The occurrence of a dominant haplotype shared amongst M. fascicularis from southern Thailand, the Malay Peninsula, and Sumatra could be the result of a recent dispersal event from the common Pleistocene refugia, which had most likely been located in the Malay Peninsula. A combination of nucleotides at 12 sites distinguished the M. fascicularis from the northern region of the Isthmus of Kra from those of the southern region including our samples. The molecular phylogenetic tree confirmed that, unlike conspecific populations from regions north of the Isthmus of Kra, M. fascicularis from the southern region of the isthmus were free of introgression of Y-chromosome from M. mulatta. We dated the last common ancestors shared by the fascicularis group at approximately 1.5 million years ago (mya). Also, we estimated the bifurcation between the insular and the continental lineages of M. fascicularis as approximately 0.7 mya, which had been estimated as 0.4 mya in a previous study. Finally, similarly to the observations on various taxa by previous authors we recognise the role of the Isthmus of Kra area as a genetic barrier to the dispersal of and to gene flow in M. fascicularis.

Published
2013

48. Is There a Risk of Suburban Transmission of Malaria in Selangor, Malaysia?

Author

Braima, Kamil A., primary, Sum, Jia-Siang, additional, Ghazali, Amir-Ridhwan M., additional, Muslimin, Mustakiza, additional, Jeffery, John, additional, Lee, Wenn-Chyau, additional, Shaker, Mohammed R., additional, Elamin, Alaa-Eldeen M., additional, Jamaiah, Ibrahim, additional, Lau, Yee-Ling, additional, Rohela, Mahmud, additional, Kamarulzaman, Adeeba, additional, Sitam, Frankie, additional, Mohd-Noh, Rosnida, additional, and Abdul-Aziz, Noraishah M., additional

Published
2013
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49. Chikungunya Virus in Macaques, Malaysia.

Author

I-Ching Sam, Chong Long Chua, Rovie-Ryan, Jeffrine J., Jolene Y.L. Fu, Charmaine Tong, Sitam, Frankie Thomas, and Yoke Fun Chan

Subjects
CHIKUNGUNYA virus, CHIKUNGUNYA, MACAQUES, SERUM, DISEASES
Abstract

This article presents a study on the chikungunya virus (CHIKV) in long-tailed macaques in Malaysia. It discusses the population of this species in the country, collection of serum samples from macaques, the use of a seroneutralization assay to screen for CHIKV neutralizing antibodies, and findings on the seroprevalence rates.

Published
2015
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50. Rickettsial Infections in Monkeys, Malaysia.

Author

Sun Tee Tay, Fui Xian Koh, Kai Ling Kho, and Sitam, Frankie Thomas

Subjects
KRA, RICKETTSIA, RICKETTSIAL diseases, ANAPLASMA, ANIMAL diseases
Abstract

The article presents a study which examined Malaysian cynomolgus monkeys, Macaca fascicularis, with Rickettsia felis-type strain URRWXCal2. Rickettsial infection was confirmed in these animals after blood analysis, DNA extraction from the blood samples and polymerase chain reaction (PCR) selective for the ricketssial citrate synthase gene. The researchers identified Candidatus Rickettsia asemboensis and Rickettsia spp. clone 4G/JP 102 and 11TP21, and Anaplasma bovis.

Published
2015
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