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8 results on '"Sitalakshmi, R."'

3. Civil construction work: The unseen contributor to the occupational and global disease burden

Author

Sitalakshmi, R., Saikumar, P., Jeyachandran, P., Manoharan, Thangavel, and Thomas, Jayakar

Subjects
Construction workers -- Health aspects, Construction industry -- Labor relations, Skin lesions -- Health aspects, Cements (Building materials) -- Usage -- Health aspects, Environmental issues, Health
Abstract

Byline: R. Sitalakshmi, P. Saikumar, P. Jeyachandran, Manoharan, Thangavel, Jayakar. Thomas Background: Construction industry is the second largest employment giving industry in India with many semi-skilled or unskilled workers taking [...]

Published
2016

4. Inositol pyrophosphates regulate RNA polymerase I-mediated rRNA transcription in Saccharomyces cerevisiae

Author

Swarna Gowri Thota, C P Unnikannan, Sitalakshmi R. Thampatty, R. Manorama, and Rashna Bhandari

Subjects
WT, wild type, Transcription, Genetic, Paromomycin, IP6, inositol hexakisphosphate, Ribosome biogenesis, Biochemistry, Ribosome, 1PP-IP5/1-IP7, 1-diphosphoinositol pentakisphosphate, chemistry.chemical_compound, RNA Polymerase I, Gene Expression Regulation, Fungal, Inositol, YPD, yeast extract peptone dextrose, CDK, cyclin-dependent kinase, Protein Synthesis Inhibitors, 1,5[PP]2-IP4/IP8 bis-diphosphoinositol pentakisphosphate, Pol I, RNA polymerase I, UAF, upstream activating factor, Translation (biology), NTP, nucleoside triphosphate, ITS, internal transcribed spacer, Research Article, Signal Transduction, Saccharomyces cerevisiae Proteins, pyrophosphorylation, Inositol Phosphates, Saccharomyces cerevisiae, Biology, NTS, non-transcribed spacer, inositol hexakisphosphate kinase, 6AU, 6-azauracil, SC, synthetic complete, RNA polymerase I, Molecular Biology, 5PP-IP5/5-IP7, 5-diphosphoinositol pentakisphosphate, SC−Ura, synthetic complete medium without uracil, Phosphotransferases (Phosphate Group Acceptor), Genetic Complementation Test, IP7, diphosphoinositol pentakisphosphate, Cell Biology, Kcs1, DEPC, diethyl pyrocarbonate, biology.organism_classification, RRNA transcription, diphosphoinositol pentakisphosphate, SC−Met, synthetic complete medium without methionine, Protein Subunits, chemistry, Ribosome Subunits, inositol pyrophosphate, RNA, Ribosomal, ETS, external transcribed spacer, Protein Biosynthesis, qPCR, quantitative PCR, Hygromycin B, Ribosomes
Abstract

Ribosome biogenesis is an essential cellular process regulated by the metabolic state of a cell. We examined whether inositol pyrophosphates, energy-rich derivatives of inositol that act as metabolic messengers, play a role in ribosome synthesis in the budding yeast, Saccharomyces cerevisiae. Yeast strains lacking the inositol hexakisphosphate (IP6) kinase Kcs1, which is required for the synthesis of inositol pyrophosphates, display increased sensitivity to translation inhibitors and decreased protein synthesis. These phenotypes are reversed on expression of enzymatically active Kcs1, but not on expression of the inactive form. The kcs1Δ yeast cells exhibit reduced levels of ribosome subunits, suggesting that they are defective in ribosome biogenesis. The rate of rRNA synthesis, the first step of ribosome biogenesis, is decreased in kcs1Δ yeast strains, suggesting that RNA polymerase I (Pol I) activity may be reduced in these cells. We determined that the Pol I subunits, A190, A43 and A34.5, can accept a β-phosphate moiety from inositol pyrophosphates to undergo serine pyrophosphorylation. Although there is impaired rRNA synthesis in kcs1Δ yeast cells, we did not find any defect in recruitment of Pol I on rDNA, but observed that the rate of transcription elongation was compromised. Taken together, our findings highlight inositol pyrophosphates as novel regulators of rRNA transcription., Inositol pyrophosphates are phosphate-rich metabolic messengers that regulate many cellular processes. We observed that RNA polymerase I is pyrophosphorylated by inositol pyrophosphates, and its transcription elongation activity was reduced in budding yeast strains devoid of inositol pyrophosphates.

Published
2015

7. Study of the Effect of Stress on Skeletal Muscle Function in Geriatrics.

Author

POORNIMA, K. N., KARTHICK, N., and SITALAKSHMI, R.

Subjects
GERIATRICS, SKELETAL muscle, PHYSIOLOGICAL stress, MUSCLE strength, BONE fractures
Abstract

Background: Old age is associated with weakness of skeletal muscles and decrease in muscle functions. Usually in old-age, people undergo wasting of muscles, so they are more prone for fall and fracture. It has been stated that stress and cognition has an impact on muscle functions. This study was intended to demonstrate the effect of stress in muscle function in geriatrics. Methods: This was a cross sectional study done at a charitable home in Chennai sub urban. The geriatric males and females in the old age home were included in this study. Sixty-four subjects were included and the persons with previous history of musculo skeletal and neurological disorders were excluded. Anthropometric parameters were recorded Maximum Voluntary Contraction (MVC) and Endurance Time (ET) were measured by hand grip dynamometer. Perceived stress score was measured by perceived stress scale questionnaire. Muscle function parameters and stress score was compared. Results: Sixty-four subjects were included in this study and it was found out that there is a negative correlation between MVC & stress which was statistically significant. (r = -0.0675, P = 0.000). Age with MVC & ET showed a mild negative correlation but it was not significant. Conclusion: Hormones released during stress have a negative metabolic effect in skeletal muscle. Stress can induce earlier decline in muscle strength which will eventually lead to fall and fracture. Therefore, stress should be viewed as an independent risk factor for disability and other co morbid conditions. [ABSTRACT FROM AUTHOR]

Published
2014
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8. Predicted equations for pulmonary function in normal adolescent south Indian population.

Author

Sitalakshmi R, Shankar RP, Padmavathi R, Subhashini AS, and Thanasekaran MV

Subjects
Adolescent, Age Factors, Child, Female, Forced Expiratory Volume, Humans, Male, Vital Capacity, Lung physiology
Abstract

Pulmonary functions are affected by variables like age, sex, height, weight, and geographic location. Our study aims to establish predicted, equations for pulmonary functions in normal South Indian adolescent population. 400 subjects were grouped into pre & peripubertal (10-14 years) and pubertal (15 to 18 years) age categories. Anthropometric data collected, PFT assessed using portable data logging Spirometer MIR II. Mean FVC and FEV1 values were 2.80 L, 2.49 L in boys and 2.34 L, 2.12 L in girls respectively. Predicted equations for both adolescent age groups were generated by using linear regression analysis. PFT were significantly different in both age categories in boys and girls. PFT increased with increasing age and significantly correlated with the anthropometric parameters. Region specific and age specific predicted equations for PFT are generated from this study.

Published
2014

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