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3. Anaerobic Profile of Intra-abdominal Infections – A 23-Year Retrospective Study

Author

Meghna Chetty, Biswas Rakhi, Sistla Sujatha, and Sistla Sarath Chandra

Subjects
anaerobes, bacteroides fragilis group, peptostreptococcus sp, metronidazole, Microbiology, QR1-502
Abstract

Obligate anaerobes, which are part of normal intestinal flora are now gaining pathogenic potential by becoming more virulent and causing moderate to severe abdominal infections. Moreover, there is delay in initiation of appropriate antimicrobial therapy. The study aimed to describe and analyse 23 years data on anaerobic intra – abdominal infections in regards to the distribution and antimicrobial susceptibility patterns of the obligate anaerobes which were isolated from various intra – abdominal infections. The demographic and microbiological data was retrieved from the microbiology departmental registers. Total number of cases/specimen were 1124. Bacteroides fragilis group (238) (56%) and Peptostreptococcus sp (109) (25%) amounted to the majority of the isolates. Rare anaerobes like Clostridium sporogenes, Propionibacterium sp, Clostridium bifermentans and Fusobacterium varium were also isolated. Majority of mixed anaerobic infections were contributed by Bacteroides fragilis group and Peptostreptococcus sp (99) out of 102 mixed anaerobic infections). Chronic alcoholism was the most common predisposing condition (p value

Published
2023
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5. Global, regional, and national disease burden estimates of acute lower respiratory infections due to respiratory syncytial virus in children younger than 5 years in 2019: a systematic analysis

Author

Abram, Michael, Aerssens, Jeroen, Alafaci, Annette, Balmaseda, Angel, Bandeira, Teresa, Barr, Ian, Batinović, Ena, Beutels, Philippe, Bhiman, Jinal, Blyth, Christopher C, Bont, Louis, Bressler, Sara S, Cohen, Cheryl, Cohen, Rachel, Costa, Anna-Maria, Crow, Rowena, Daley, Andrew, Dang, Duc-Anh, Demont, Clarisse, Desnoyers, Christine, Díez-Domingo, Javier, Divarathna, Maduja, du Plessis, Mignon, Edgoose, Madeleine, Ferolla, Fausto Martín, Fischer, Thea K, Gebremedhin, Amanuel, Giaquinto, Carlo, Gillet, Yves, Hernandez, Roger, Horvat, Come, Javouhey, Etienne, Karseladze, Irakli, Kubale, John, Kumar, Rakesh, Lina, Bruno, Lucion, Florencia, MacGinty, Rae, Martinon-Torres, Federico, McMinn, Alissa, Meijer, Adam, Milić, Petra, Morel, Adrian, Mulholland, Kim, Mungun, Tuya, Murunga, Nickson, Newbern, Claire, Nicol, Mark P, Odoom, John Kofi, Openshaw, Peter, Ploin, Dominique, Polack, Fernando P, Pollard, Andrew J, Prasad, Namrata, Puig-Barberà, Joan, Reiche, Janine, Reyes, Noelia, Rizkalla, Bishoy, Satao, Shilpa, Shi, Ting, Sistla, Sujatha, Snape, Matthew, Song, Yanran, Soto, Giselle, Tavakoli, Forough, Toizumi, Michiko, Tsedenbal, Naranzul, van den Berge, Maarten, Vernhes, Charlotte, von Mollendorf, Claire, Walaza, Sibongile, Walker, Gregory, Li, You, Wang, Xin, Blau, Dianna M, Caballero, Mauricio T, Feikin, Daniel R, Gill, Christopher J, Madhi, Shabir A, Omer, Saad B, Simões, Eric A F, Campbell, Harry, Pariente, Ana Bermejo, Bardach, Darmaa, Bassat, Quique, Casalegno, Jean-Sebastien, Chakhunashvili, Giorgi, Crawford, Nigel, Danilenko, Daria, Do, Lien Anh Ha, Echavarria, Marcela, Gentile, Angela, Gordon, Aubree, Heikkinen, Terho, Huang, Q Sue, Jullien, Sophie, Krishnan, Anand, Lopez, Eduardo Luis, Markić, Joško, Mira-Iglesias, Ainara, Moore, Hannah C, Moyes, Jocelyn, Mwananyanda, Lawrence, Nokes, D James, Noordeen, Faseeha, Obodai, Evangeline, Palani, Nandhini, Romero, Candice, Salimi, Vahid, Satav, Ashish, Seo, Euri, Shchomak, Zakhar, Singleton, Rosalyn, Stolyarov, Kirill, Stoszek, Sonia K, von Gottberg, Anne, Wurzel, Danielle, Yoshida, Lay-Myint, Yung, Chee Fu, Zar, Heather J, and Nair, Harish

Published
2022
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10. Severe acute kidney injury in a 3-year-old boy with fever and pleural effusion: Answers

Author

Chidambaram, Aakash Chandran, Krishnamurthy, Sriram, Deepthi, Bobbity, Karunakar, Pediredla, Maulik, Kaushik, Peela, Sreeram Chandra Murthy, and Sistla, Sujatha

Subjects
Children -- Diseases, Hemolytic-uremic syndrome -- Case studies -- Diagnosis -- Complications and side effects, Acute renal failure -- Case studies -- Diagnosis -- Causes of, Pediatric research, Acute renal failure in children -- Case studies -- Diagnosis -- Causes of, Health
Abstract

Author(s): Aakash Chandran Chidambaram [sup.1] , Sriram Krishnamurthy [sup.1] , Bobbity Deepthi [sup.1] , Pediredla Karunakar [sup.1] , Kaushik Maulik [sup.1] , Sreeram Chandra Murthy Peela [sup.2] , Sujatha Sistla [...]

Published
2020
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13. Global patterns in monthly activity of influenza virus, respiratory syncytial virus, parainfluenza virus, and metapneumovirus: a systematic analysis

Author

Acacio, Sozinho, Alonso, Wladimir J, Antonio, Martin, Ayora Talavera, Guadalupe, Badarch, Darmaa, Baillie, Vicky L, Barrera-Badillo, Gisela, Bigogo, Godfrey, Broor, Shobha, Bruden, Dana, Buchy, Philippe, Byass, Peter, Chipeta, James, Clara, Wilfrido, Dang, Duc-Anh, de Freitas Lázaro Emediato, Carla Cecília, de Jong, Menno, Díaz-Quiñonez, José Alberto, Do, Lien Anh Ha, Fasce, Rodrigo A, Feng, Luzhao, Ferson, Mark J, Gentile, Angela, Gessner, Bradford D, Goswami, Doli, Goyet, Sophie, Grijalva, Carlos G, Halasa, Natasha, Hellferscee, Orienka, Hessong, Danielle, Homaira, Nusrat, Jara, Jorge, Kahn, Kathleen, Khuri-Bulos, Najwa, Kotloff, Karen L, Lanata, Claudio F, Lopez, Olga, Lopez Bolaños, Maria Renee, Lucero, Marilla G, Lucion, Florencia, Lupisan, Socorro P, Madhi, Shabir A, Mekgoe, Omphile, Moraleda, Cinta, Moyes, Jocelyn, Mulholland, Kim, Munywoki, Patrick K, Naby, Fathima, Nguyen, Thanh Hung, Nicol, Mark P, Nokes, D James, Noyola, Daniel E, Onozuka, Daisuke, Palani, Nandhini, Poovorawan, Yong, Rahman, Mustafizur, Ramaekers, Kaat, Romero, Candice, Schlaudecker, Elizabeth P, Schweiger, Brunhilde, Seidenberg, Phil, Simoes, Eric A F, Singleton, Rosalyn, Sistla, Sujatha, Sturm-Ramirez, Katharine, Suntronwong, Nungruthai, Sutanto, Agustinus, Tapia, Milagritos D, Thamthitiwat, Somsak, Thongpan, Ilada, Tillekeratne, Gayani, Tinoco, Yeny O, Treurnicht, Florette K, Turner, Claudia, Turner, Paul, van Doorn, Rogier, Van Ranst, Marc, Visseaux, Benoit, Waicharoen, Sunthareeya, Wang, Jianwei, Yoshida, Lay-Myint, Zar, Heather J, Li, You, Reeves, Rachel M, Wang, Xin, Bassat, Quique, Brooks, W Abdullah, Cohen, Cheryl, Moore, David P, Nunes, Marta, Rath, Barbara, Campbell, Harry, and Nair, Harish

Published
2019
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16. Structure Elucidation and Interaction Dynamics of MefA-MsrD Efflux Proteins in Streptococcus pneumoniae: Impact on Macrolide Susceptibility

Author

Peela, Sreeram Chandra Murthy, primary, Basu, Soumya, additional, Sharma, Jyoti, additional, AlAsmari, Abdullah F., additional, AlAsmari, Fawaz, additional, Alalmaee, Sultan, additional, Ramaiah, Sudha, additional, Sistla, Sujatha, additional, Livingstone, Paul, additional, and Anbarasu, Anand, additional

Published
2023
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28. Clinical profile, visual outcome and root cause analysis of post-operative cluster endophthalmitis due to Burkholderia cepacia complex

Author

Deb, Amit, Chavan, Pratima, Kaliaperumal, Subashini, Sistla, Sujatha, Madigubba, Haritha, Sarkar, Sandip, and Neena, Aswathi

Subjects
Opportunistic infections -- Drug therapy -- Causes of -- Patient outcomes, Endophthalmitis -- Care and treatment -- Patient outcomes -- Causes of -- Drug therapy, Gram-negative bacterial infections -- Patient outcomes -- Causes of -- Drug therapy, Health
Abstract

Byline: Amit. Deb, Pratima. Chavan, Subashini. Kaliaperumal, Sujatha. Sistla, Haritha. Madigubba, Sandip. Sarkar, Aswathi. Neena Purpose: To present varied clinical presentations, surveillance reports, and final visual outcomes of a rare [...]

Published
2022

29. Effect of combined colistin and meropenem against meropenem resistant Acinetobacter baumannii and Pseudomonas aeruginosa by checkerboard method: A cross sectional analytical study

Author

Gunalan, Anitha, Sarumathi, Dhandapani, Sastry, Apurba, Ramanathan, Venkateswaran, Rajaa, Sathish, and Sistla, Sujatha

Subjects
Colistin -- Properties, Drug resistance in microorganisms -- Analysis, Acinetobacter baumannii -- Identification and classification -- Physiological aspects, Pseudomonas aeruginosa -- Identification and classification -- Physiological aspects, Health
Abstract

Byline: Anitha. Gunalan, Dhandapani. Sarumathi, Apurba. Sastry, Venkateswaran. Ramanathan, Sathish. Rajaa, Sujatha. Sistla BACKGROUND: Meropenem-resistant Acinetobacter baumannii and Pseudomonas aeruginosa are the two most common nosocomial pathogens causing ventilator-associated pneumonia. [...]

Published
2021

34. Worrisome trends in rising minimum inhibitory concentration values of antibiotics against methicillin resistant Staphylococcus aureus – Insights from a tertiary care center, South India

Author

Nagasundaram Niveditha and Sistla Sujatha

Subjects
Infectious and parasitic diseases, RC109-216, Microbiology, QR1-502
Abstract

Introduction: Appearance of isolated reports of resistance to anti-methicillin-resistant Staphylococcus aureus (MRSA) drugs is worrisome underscoring the need to continuously monitor the susceptibility of clinical MRSA isolates to these drugs. Hence, the present study is conducted to determine the susceptibility of MRSA isolates to various classes of anti-MRSA drugs such as vancomycin (glycopeptide), daptomycin (lipopeptide), tigecycline (glycylcycline), and linezolid (oxazolidinone) to determine the MIC50 and MIC90 values, and to observe MIC creep over a three year period, if any, with respect to these drugs. Methods: A total of 200 isolates of MRSA obtained from clinical specimens were included. MIC was determined by E-test for anti-MRSA antibiotics vancomycin, linezolid, daptomycin, and tigecycline. Non-parametric methods (Kruskal–Wallis and Chi-square test) were used to assess MIC trends over time. In addition, MIC50 and MIC90 values were also calculated. Results: No isolate was found resistant to vancomycin, daptomycin, or linezolid; five isolates were resistant to tigecycline. Seven VISA isolates were encountered with the MIC value for vancomycin of 4 μg/mL. MIC values for vancomycin, tigecycline, linezolid showed a definite increase over a 3-year period which was statistically significant with p-values

Published
2015
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35. SERUM LEVELS OF COPPER AND IRON IN DENGUE FEVER

Author

Rajendiran SOUNDRAVALLY, Jacob SHERIN, Balakrishna Pillai AGIESHKUMAR, Mariya Samadanam DAISY, Cherupanakkal CLEETUS, Parameswaran NARAYANAN, Tamilarasu KADHIRAVAN, Sistla SUJATHA, and Kottyen Thazhath HARICHANDRAKUMAR

Subjects
Copper, Iron, Trace elements, Dengue severity, Dengue fever, Arctic medicine. Tropical medicine, RC955-962, Infectious and parasitic diseases, RC109-216
Abstract

SUMMARY The role of trace elements in dengue virulence is not yet known. The present study assessed the serum levels of two micronutrients, copper and iron, in cases of dengue fever. The study involved 96 patients of whom 48 had either severe or non-severe forms of dengue (with and without warning signs), and the remaining 48 were patients with other febrile illnesses (OFI), used as controls. Serum levels of copper and iron were evaluated at admission and by the time of defervescence using commercially available kits. At admission, no difference in the level of serum copper was observed between cases and controls. In the group of dengue cases, the copper level was found to be significantly decreased in severe and non-severe cases with warning signs, compared to non-severe cases without warning signs. In contrast, by the time of defervescence the copper level was found to be increased in all dengue cases compared to OFI controls, but no difference was observed among dengue cases. Unlike OFI controls, dengue cases showed an increasing pattern of copper levels from admission until defervescence. On the other hand, no such significant differences were observed in the serum level of iron in the clinical groups, except for a decreased iron level found in severe cases, compared to non-severe dengue without warning signs. The results show that copper is associated with dengue severity and this finding emphasizes the need to investigate the involvement of trace elements in disease severity so as to improve the prognosis of dengue.

Published
2015
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41. Endophthalmitis due to Delftia acidovorans: An unusual ocular pathogen

Author

Deb, Amit, Chavhan, Pratima, Chowdhury, Sushmita, Sistla, Sujatha, Sugumaran, Radha, and Panicker, Gayathri

Subjects
Gram-negative bacterial infections -- Case studies -- Complications and side effects -- Care and treatment, Endophthalmitis -- Case studies -- Development and progression -- Care and treatment, Health
Abstract

Byline: Amit. Deb, Pratima. Chavhan, Sushmita. Chowdhury, Sujatha. Sistla, Radha. Sugumaran, Gayathri. Panicker Endophthalmitis is a dreaded postoperative complication of cataract surgery. Delftia acidovorans is usually nonpathogenic and an unusual [...]

Published
2020

42. Poor Sensorium at the Time of Intubation Predicts Polymicrobial Ventilator Associated Pneumonia

Author

Natarajan,Ramachandran, Ramanathan,Venkateswaran, Sistla,Sujatha, Natarajan,Ramachandran, Ramanathan,Venkateswaran, and Sistla,Sujatha

Abstract

Ramachandran Natarajan,1 Venkateswaran Ramanathan,1 Sujatha Sistla2 1Department of Medicine, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India; 2Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, IndiaCorrespondence: Venkateswaran Ramanathan, Department of Medicine, Jawaharlal Institute of Postgraduate Medical Education and Research, Dhanvantri Nagar, Puducherry, 605006, India, Email drvenks@gmail.comBackground: Ventilator-associated pneumonia (VAP) is the most common nosocomial infection in the intensive care unit and is associated with a high mortality rate.Aim: The study was conducted to estimate the frequency, outcomes, and predictors of polymicrobial VAP.Methods: A prospective observational study was conducted in mechanically ventilated adult patients in the medical intensive care unit in a tertiary care hospital in India from July 2016 to July 2018 with a 30-day follow-up period. The patients were grouped into monomicrobial and polymicrobial VAP. We compared the 30-day outcome parameters such as discharge from hospital, in-hospital stay, death, and complications such as catheter associated urinary tract infection (CAUTI), central line associated blood stream infection (CRBSI), bacteremia and collapse of lung. The predictors of polymicrobial VAP were identified by multiple logistic regression.Results: Out of 301 patients clinically diagnosed with VAP, 151 patients were excluded, and the remaining 150 developed 186 episodes of VAP during the study period. The incidence of polymicrobial VAP was 62.9%. Out of 150 patients, 51 patients had monomicrobial VAP, and 99 had polymicrobial VAP. On univariate analysis, diabetes mellitus and poor sensorium (Glasgow Coma Scale [GCS] score < 8) during endotracheal intubation; 30-day outcome, mean days of mechanical ventilation after VAP diagnosis and days in ICU; and CAUTI were significantly associated with polymicrobial VAP. On

Published
2022

43. Virulence factors in clinical and commensal isolates of Enterococcus species

Author

Praharaj Ira, Sistla Sujatha, and Parija Subhash Chandra

Subjects
Biofilms, Enterococcus, hemolysin, gelatinase, polymerase chain reaction, virulence, Pathology, RB1-214, Microbiology, QR1-502
Abstract

Background: Enterococci have emerged as important nosocomial pathogens and have been found to possess many virulence factors, some of which are considered very important in the pathogenesis of diseases caused by them. The following study was carried out to evaluate some of the virulence determinants elaborated by strains of enterococci in our setup and to ascertain if these strains differ considerably from commensal strains of enterococci in the expression of these virulence determinants. Materials and Methods: One hundred and fifty-seven isolates of Enterococcus species from clinical specimens were evaluated for the presence of virulence determinants like hemolysin production, gelatinase production and biofilm formation by phenotypic tests. The presence of enterococcal surface protein (esp) gene in the isolates was detected using polymerase chain reaction (PCR). Thirty strains of Enterococcus isolated from fecal samples of patients admitted to the hospital were also tested for the presence of these virulence factors. Strains of Enterococcus from clinical specimens and those present as commensals were compared with respect to the elaboration of virulence factors using Fisher′s exact test. Results: The association between biofilm formation and presence of the "esp" gene was not found to be statistically significant. Among the virulence determinants studied, gelatinase production and the "esp" gene were found to be significantly more common in clinical isolates than commensal strains of Enterococcus species. Conclusion: Among the virulence factors, gelatinase and the "esp" gene were more common in clinical isolates than commensal strains. The association between biofilm formation and the presence of "esp" gene was not found to be statistically significant.

Published
2013
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45. Global, regional, and national disease burden estimates of acute lower respiratory infections due to respiratory syncytial virus in children younger than 5 years in 2019: a systematic analysis

Author

Li, You, primary, Wang, Xin, additional, Blau, Dianna M, additional, Caballero, Mauricio T, additional, Feikin, Daniel R, additional, Gill, Christopher J, additional, Madhi, Shabir A, additional, Omer, Saad B, additional, Simões, Eric A F, additional, Campbell, Harry, additional, Pariente, Ana Bermejo, additional, Bardach, Darmaa, additional, Bassat, Quique, additional, Casalegno, Jean-Sebastien, additional, Chakhunashvili, Giorgi, additional, Crawford, Nigel, additional, Danilenko, Daria, additional, Do, Lien Anh Ha, additional, Echavarria, Marcela, additional, Gentile, Angela, additional, Gordon, Aubree, additional, Heikkinen, Terho, additional, Huang, Q Sue, additional, Jullien, Sophie, additional, Krishnan, Anand, additional, Lopez, Eduardo Luis, additional, Markić, Joško, additional, Mira-Iglesias, Ainara, additional, Moore, Hannah C, additional, Moyes, Jocelyn, additional, Mwananyanda, Lawrence, additional, Nokes, D James, additional, Noordeen, Faseeha, additional, Obodai, Evangeline, additional, Palani, Nandhini, additional, Romero, Candice, additional, Salimi, Vahid, additional, Satav, Ashish, additional, Seo, Euri, additional, Shchomak, Zakhar, additional, Singleton, Rosalyn, additional, Stolyarov, Kirill, additional, Stoszek, Sonia K, additional, von Gottberg, Anne, additional, Wurzel, Danielle, additional, Yoshida, Lay-Myint, additional, Yung, Chee Fu, additional, Zar, Heather J, additional, Nair, Harish, additional, Abram, Michael, additional, Aerssens, Jeroen, additional, Alafaci, Annette, additional, Balmaseda, Angel, additional, Bandeira, Teresa, additional, Barr, Ian, additional, Batinović, Ena, additional, Beutels, Philippe, additional, Bhiman, Jinal, additional, Blyth, Christopher C, additional, Bont, Louis, additional, Bressler, Sara S, additional, Cohen, Cheryl, additional, Cohen, Rachel, additional, Costa, Anna-Maria, additional, Crow, Rowena, additional, Daley, Andrew, additional, Dang, Duc-Anh, additional, Demont, Clarisse, additional, Desnoyers, Christine, additional, Díez-Domingo, Javier, additional, Divarathna, Maduja, additional, du Plessis, Mignon, additional, Edgoose, Madeleine, additional, Ferolla, Fausto Martín, additional, Fischer, Thea K, additional, Gebremedhin, Amanuel, additional, Giaquinto, Carlo, additional, Gillet, Yves, additional, Hernandez, Roger, additional, Horvat, Come, additional, Javouhey, Etienne, additional, Karseladze, Irakli, additional, Kubale, John, additional, Kumar, Rakesh, additional, Lina, Bruno, additional, Lucion, Florencia, additional, MacGinty, Rae, additional, Martinon-Torres, Federico, additional, McMinn, Alissa, additional, Meijer, Adam, additional, Milić, Petra, additional, Morel, Adrian, additional, Mulholland, Kim, additional, Mungun, Tuya, additional, Murunga, Nickson, additional, Newbern, Claire, additional, Nicol, Mark P, additional, Odoom, John Kofi, additional, Openshaw, Peter, additional, Ploin, Dominique, additional, Polack, Fernando P, additional, Pollard, Andrew J, additional, Prasad, Namrata, additional, Puig-Barberà, Joan, additional, Reiche, Janine, additional, Reyes, Noelia, additional, Rizkalla, Bishoy, additional, Satao, Shilpa, additional, Shi, Ting, additional, Sistla, Sujatha, additional, Snape, Matthew, additional, Song, Yanran, additional, Soto, Giselle, additional, Tavakoli, Forough, additional, Toizumi, Michiko, additional, Tsedenbal, Naranzul, additional, van den Berge, Maarten, additional, Vernhes, Charlotte, additional, von Mollendorf, Claire, additional, Walaza, Sibongile, additional, and Walker, Gregory, additional

Published
2022
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