1. Single-cell profiling reveals sex diversity in human renal proximal tubules
- Author
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Jinling Liao, Xiaobo Yang, Lin Huang, Juan He, Haiying Zhang, Jiwen Cheng, Yang Chen, Siqiong Pan, and Zengnan Mo
- Subjects
Male ,0301 basic medicine ,Cell ,Organic Anion Transporters, Sodium-Independent ,Biology ,Kidney ,Kidney Tubules, Proximal ,Transcriptome ,Mice ,03 medical and health sciences ,Organic Anion Transport Protein 1 ,Sex Factors ,0302 clinical medicine ,Gene expression ,Genetics ,medicine ,Animals ,Humans ,Gene ,Sequence Analysis, RNA ,RNA ,Transporter ,General Medicine ,Cell biology ,Mice, Inbred C57BL ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Mouse Kidney ,Female ,Single-Cell Analysis - Abstract
Many anatomical regions in the kidney, including proximal tubules, differ between males and females. While such differences in renal structures and functions under various physiological and pharmacological conditions have been identified, information relating to molecular mechanisms behind this gender disparity remain unknown. To understand gene expression differences in proximal tubules from human male and female kidneys, we reported on kidney cellular landscape using single-cell RNA sequencing. Differential gene expression profiles were observed in proximal tubules, between the sexes. Interestingly, the SLC22 family of anion transporters, including SLC22A6 and SLC22A8, had different expression profiles between male and female proximal tubule clusters but not sex-dependent abundance at the protein level. Moreover, in different species, we revealed a shared and species-specific differential gene expression between human and mouse kidney proximal tubules. Taken together, at single-cell resolution, this transcriptomic map represents a baseline description of gender biased genes in human kidney proximal tubules, which provide important insights for further studies of physiological differences in kidney.
- Published
- 2020