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15 results on '"Singh, Rahul Mohan"'

4. Molecular evidence of Apple Stem Grooving Virusinfecting Pear in India

Author

Bhardwaj, Pooja, Singh, Rahul Mohan, and Hallan, Vipin

Abstract

Pear leaf samples exhibiting severe virus-like symptoms (such as distortion, chlorosis, chlorotic spots, marginal chlorosis and necrotic spots) were collected from the pome and stone fruit germplasm fields of CSIR-IHBT campus and Horticulture department of CSKHPKV Palampur (Himachal Pradesh). The leaf samples collected from 46 plants belonging to three different cultivars (Kashmiri pear, babhugosha and patharnakh) were subject to virus indexing by DAS ELISA, dot blot hybridization and validated by RT-PCR. Total three samples found positive were used for the characterization of complete CP gene of the virus. The three isolates characterized from the pear (HPPR-2, HPPR-3 and HPPR-46) shared 93.2–96.6% sequence identity (at amino acid level) among themselves and in the phylogenetic tree these three isolates grouped in separate clusters. The sequence identity (at amino acid level) with rest of the pear isolates characterized from other countries like South Korea, Japan, China and Taiwan was found to range from 95.7 to 97.4% in case of HPPR-2, 94-95.3% for HPPR-3 and 94.9–97.4% for HPPR-46.

Published
2023
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15. The m6A reader ECT1 drives mRNA sequestration to dampen salicylic acid-dependent stress responses in Arabidopsis.

Author

Lee KP, Liu K, Kim EY, Medina-Puche L, Dong H, Di M, Singh RM, Li M, Qi S, Meng Z, Cho J, Zhang H, Lozano-Duran R, and Kim C

Subjects
Salicylic Acid metabolism, RNA, Messenger genetics, RNA, Messenger metabolism, Gene Expression Regulation, Plant, Arabidopsis metabolism, Arabidopsis Proteins genetics, Arabidopsis Proteins metabolism, Adenine analogs & derivatives
Abstract

N 6-methyladenosine (m6A) is a common epitranscriptional mRNA modification in eukaryotes. Thirteen putative m6A readers, mostly annotated as EVOLUTIONARILY CONSERVED C-TERMINAL REGION (ECT) proteins, have been identified in Arabidopsis (Arabidopsis thaliana), but few have been characterized. Here, we show that the Arabidopsis m6A reader ECT1 modulates salicylic acid (SA)-mediated plant stress responses. ECT1 undergoes liquid-liquid phase separation in vitro, and its N-terminal prion-like domain is critical for forming in vivo cytosolic biomolecular condensates in response to SA or bacterial pathogens. Fluorescence-activated particle sorting coupled with quantitative PCR analyses unveiled that ECT1 sequesters SA-induced m6A modification-prone mRNAs through its conserved aromatic cage to facilitate their decay in cytosolic condensates, thereby dampening SA-mediated stress responses. Consistent with this finding, ECT1 overexpression promotes bacterial multiplication in plants. Collectively, our findings unequivocally link ECT1-associated cytosolic condensates to SA-dependent plant stress responses, advancing the current understanding of m6A readers and the SA signaling network., Competing Interests: Conflict of interest statement. None declared., (© The Author(s) 2023. Published by Oxford University Press on behalf of American Society of Plant Biologists. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

Published
2024
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