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Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a rare and clinically aggressive hematologic malignancy. Although BPDCN has long been recognized as a distinct clinical entity, the nomenclature for this disease [...], * Context.--Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a rare hematologic malignancy with poor outcome. BPDCN diagnostically overlaps with entities such as acute myeloid leukemia, histiocytic/dendritic cell neoplasms, and natural killer/T-cell lymphomas. Unfortunately, large, patient-centered studies that comprehensively analyze clinical, pathologic, and other diagnostic features are lacking. As such, there is an incomplete understanding of this disease. Objective.--To better characterize BPDCN, a multicenter working group consisting of hematopathologists and dermatopathologists gathered in person and remotely to review the current understanding of BPDCN, discuss specific issues regarding the diagnosis and differential diagnosis, and perform a retrospective analysis of the literature. Data Sources.--The working group curated a database of published BPDCN patient cases (BPDCN Network literature database), and following careful discussion and review, 361 articles were identified, comprising a total of 1513 individually annotated patients. Conclusions.--By conducting an in-depth analysis, not only did we confirm known findings such as frequent skin involvement (84% of patients; 861 of 1028) and a male predominance among older patients (>60 years old; male to female ratio of 3.5:1; 617:177), but we also identified a number of underrecognized features, such as significant central nervous system involvement (38% of cases; 24 of 64), and a more equal male to female prevalence among patients younger than 40 years (male to female ratio of 1.25:1; 167:134). Furthermore, we were able to accurately summarize the immunophenotypic, cytogenetic, and molecular features of this disease. BPDCN is a complex disease with distinct morphologic, immunophenotypic, and molecular findings. Continual updates of the literature database generated here and further analysis can allow for prospective refinement of our understanding of this orphan disease. (Arch Pathol Lab Med. 2023;147:837-846; doi: 10.5858/ arpa.2021-0612-RA)

BackgroundSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2), known to be the causative agent of COVID-19, has led to a worldwide pandemic. At presentation, individual clinical laboratory blood values, such as lymphocyte counts or C-reactive protein (CRP) levels, may be abnormal and associated with disease severity. However, combinatorial interpretation of these laboratory blood values, in the context of COVID-19, remains a challenge.MethodsTo assess the significance of multiple laboratory blood values in patients with SARS-CoV-2 and develop a COVID-19 predictive equation, we conducted a literature search using PubMed to seek articles that included defined laboratory data points along with clinical disease progression. We identified 9846 papers, selecting primary studies with at least 20 patients for univariate analysis to identify clinical variables predicting nonsevere and severe COVID-19 cases. Multiple regression analysis was performed on a training set of patient studies to generate severity predictor equations, and subsequently tested on a validation cohort of 151 patients who had a median duration of observation of 14 days.ResultsTwo COVID-19 predictive equations were generated: one using four variables (CRP, D-dimer levels, lymphocyte count, and neutrophil count), and another using three variables (CRP, lymphocyte count, and neutrophil count). In adult and pediatric populations, the predictive equations exhibited high specificity, sensitivity, positive predictive values, and negative predictive values.ConclusionUsing the generated equations, the outcomes of COVID-19 patients can be predicted using commonly obtained clinical laboratory data. These predictive equations may inform future studies evaluating the long-term follow-up of COVID-19 patients.

B-cell and T-cell lymphomas and leukemias often have distinct genetic mutations that are diagnostically defining or prognostically significant. A subset of these mutations consists of specific point mutations, which can be evaluated using genetic sequencing approaches or point mutation specific antibodies. Here, we describe genes harboring point mutations relevant to B-cell and T-cell malignancies and discuss the current availability of these targeted point mutation specific antibodies. We also evaluate the possibility of generating novel antibodies against known point mutations by computationally assessing for chemical and structural features as well as epitope antigenicity of these targets. Our results not only summarize several genetic mutations and identify existing point mutation specific antibodies relevant to hematologic malignancies, but also reveal potential underdeveloped targets which merit further study.

Water hyacinth (Pontederia crassipes) invasion is a growing global issue which poses a threat to aquatic life. As in the global context, the water hyacinth invasion is increasing rapidly in the lakes of Pokhara valley which is listed in Ramsar sites. Its effect on aquatic life is little known. This study examined the effects of water hyacinth invasion on the diversity and abundance of fishes in lakes of Pokhara Valley. We estimated the seasonal fish diversity and abundance in water hyacinth presence (HP) and absence (HA) habitats between 2019 and 2020. The study found that water hyacinth invasion has a negative impact on fish diversity and abundance in the lakes of Pokhara Valley. Twenty fish species were recorded, with family Cyprinidae being the most abundant. Fish abundance was higher in the winter season and in habitats with water hyacinth presence. The invasive Oreochromis niloticus was the most abundant fish in both habitats, but more so in habitats with water hyacinth presence. The exotic fish species Ctenopharyngodon idella had greater abundance in habitats with water hyacinth presence while Ciprinus carpio had greater abundance in habitats without water hyacinth. Exotic fish species abundance was positively correlated with water hyacinth coverage. The findings suggest that water hyacinth invasion negatively impacts native fish species, with the diversity of native species being low in habitats with water hyacinth presence. The study recommends regular stock enhancement programs for native fish species, control and management of invasive weeds, and further study to support the conservation of native fisheries resources and the livelihood of the fishery-dependent community.

Transcranial direct current stimulation (tDCS) is a non-invasive and painless technique of brain neuromodulation that applies a low-intensity galvanic current to the scalp with the aim of stimulating specific areas of the brain. Preliminary investigations have indicated the potential therapeutic efficacy of multisession tDCS applied to the auditory cortex (AC) in the treatment of chronic tinnitus. The aim of this study was to explore the therapeutic effects of repeated sessions of bilateral tDCS targeting the AC on chronic tinnitus. A double-blinded randomized placebo-controlled trial was conducted on patients (n = 48) with chronic intractable tinnitus (>2 years duration). Participants were randomly allocated to two groups: one receiving tDCS (n = 26), with the anode/cathode placed over the left/right AC, and the other receiving a placebo treatment (n = 22). A 20 min daily session of 2 mA current was administered for five consecutive days per week over two consecutive weeks, employing 35 cm2 electrodes. Tinnitus handicap inventory (THI) scores, tinnitus loudness, and tinnitus distress were measured using a visual analogue scale (VAS), and were assessed before intervention, immediately after, and at one-month follow-up. Anodal tDCS significantly reduced THI from 72.93 ± 10.11 score to 46.40 ± 15.36 after the last session and 49.68 ± 14.49 at one-month follow-up in 18 out of 25 participants (p < 0.001). The risk ratio (RR) of presenting an improvement of ≥20 points in the THI after the last session was 10.8 in patients treated with tDCS. Statistically significant reductions were observed in distress VAS and loudness VAS (p < 0.001). No statistically significant differences in the control group were observed. Variables such as age, gender, duration of tinnitus, laterality of tinnitus, baseline THI scores, and baseline distress and loudness VAS scores did not demonstrate significant correlations with treatment response. Repeated sessions of bilateral AC tDCS may potentially serve as a therapeutic modality for chronic tinnitus.

Stake voting is a voting system where the vote cast by a voter is proportional to their account balance/asset. We propose the first implementation of an efficient, fully-decentralized, universally-verifiable stake voting system with perfect voter privacy. Our contribution is two-fold. First, we design a zero knowledge proof (ZKP) for equality of plaintexts of 2 different ciphertexts encrypted under different encryption schemes, one under ElGamal encryption scheme and another under Paillier encryption scheme. We believe that ours is the first ZKP design for this problem statement. Second, we implement a publicly-verifiable Stake voting system using the ZKP design by enforcing perfect privacy to voters and correctness of election results. Our voting system is applicable to many situations like shareholder/stockholder voting in companies. We have also provided the financial and computational breakdown feasibility of our implementation on Ganache local blockchain. [ABSTRACT FROM AUTHOR]