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The goal of the current study was to develop a standardized protocol for efficient in vitroregeneration of citrus cultivars ‘Daisy’ and ‘W. Murcott’ mandarin. Treatment with 0.1% mercuric chloride (HgCl2for 5 min) and 0.5% Bavistin (10 min) effectively induced seed germination in ‘Daisy’ at 87.20%. Intermodal explants showed a high establishment rate of 98.80% in both ‘Dasiy’ and ‘W. Murcott’. Maximum shoot regeneration of 98.20% was recorded on MS fortified with 0.5 mgL−1BAP + 0.5 mgL−1Kinetin using nodal segments, followed by leaf explants (88.80%) on MS enriched with 2.0 mgL−1BAP. Highest shoot proliferation (2.6 shoots per explant) with mean shoot length of 2.3 cm was observed on MS containing BAP (2.5 mgL−1) in ‘Daisy’ mandarin whereas, highest shoot length (3.64 cm) was recorded on MS medium supplemented with 2.5 mgL−1BAP and 0.5 mgL−1GA3. However, in ‘W. Murcott’, highest shoot multiplication (2.60 shoots per explant) with 1.9 cm mean shoot length was recorded on MS + BAP (3.0 mgL−1). Whereas, maximum mean shoot length of 3.50 cm was observed on MS enriched with BAP (2.0 mgL−1) and GA3(0.5 mgL−1). Highest in vitrorooting (70.60%) with 2.3 average root number was observed on ½ MS supplemented with IBA (1.0 mgL−1) in ‘Daisy’ mandarin, in comparison to 68.40% rooting with 2.1 average number of roots in ‘W. Murcott’ on ½ MS + IBA (0.5 mgL−1). In vitrorooted plants when hardened showed 80.00% survival in ‘Daisy’ and 72.33% survival in ‘W. Murcott’ in potting mixture composed of cocopeat + vermiculite + perlite (2:1:1) after one month of hardening with no morphological variations.

Reactive blue-13 (RB-13) dye was removed using magnetic activated carbon (MAC) in the batch mode. The effect of the initial mass of the adsorbent (1–25 g/L), initial RB-13 concentration (10–50 mg/L), solution pH (2–12), temperature (298–333 K), and contact period (0–120 min) on the removal of RB-13 dye was explored. The isoelectric point study was also performed for determining the pH at which the net charge on the surface of MAC was equivalent to zero. The Langmuir adsorption isotherm showed the best correlation at all temperatures. The adsorption kinetics was best described by a pseudo-second-order model. Thermodynamic study concluded that the adsorption of RB-13 dye onto MAC took place exothermically and the process was spontaneous.