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Diabetes mellitus (DM) is a metabolic disease, which causes an increase in the proinflammatory cytokines tumor necrosis factor-? (TNF-?) and interleukin 1? (IL-1?), and also proliferation of monocyte chemotactic protein. In the present study, the potential effects of melatonin on proinflammatory cytokines, hematological values, and lymphoid tissues were investigated in diabetic rats. In the study, 36 male rats were randomly divided into 4 groups as follows: Control, Mel (melatonin), DM, and DM-Mel. For 15 days, an isotonic saline solution was given to the Control and DM groups; melatonin was administered to the Mel and DM-Mel groups intraperitoneally. At the end of the study, all animals were sacrificed by drawing the blood from their hearts under deep anesthesia. Samples of the spleen, thymus, and lymph nodes were fixed in 10 % formaldehyde for histologic analysis. Increases in proinflammatory serum cytokine concentrations, mast cells, and total white blood cell counts as well as tissue destruction in the lymphoid organs were determined in the DM group via biochemical, hematological, and histologic analyses. However, the findings for the DM-Mel group revealed decreases in serum IL-1? concentration and mast cell densities, and destructions in lymphoid tissues by the melatonin administration. The present study suggests that melatonin treatment may control immune system regulation and inhibit the production of proinflammatory cytokines and tissue mast cell accumulation by preventing the destruction of lymphoid organs in the diabetic process. [ABSTRACT FROM AUTHOR]

The aim of this study was to determine the enzyme-histochemical, histometric and hematological features of peripheral blood cells in the Sparrowhawk (Accipiter nisus). Therefore, blood samples obtained from the wings (brachial vein) of nine Sparrowhawk were used. Red blood cell (RBC) and white blood cell (WBC) counts, enumeration of leukocyte types, hematocrit (Htc) value and hemoglobin concentration were determined by hemocytometric methods on blood samples. Also, the erythrocyte indices (mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC)) were calculated. Histometric analyses were estimated with an image analyzing system. Populations of positive and negative lymphocytes were counted by the alpha naphthyl acetate esterase (ANAE) staining method. When compared to other bird species, RBC counts were high, WBC counts and percentages of WBC except percentage of eosinophil, and percentage of ANAE-positive lymphocyte were similar, and diameters of RBC and nucleus were smaller. This preliminary study contributes by broadening the hematological research on wild bird species and provides a guideline for identifying blood cells in the Sparrowhawk. [ABSTRACT FROM AUTHOR]

The article discusses a study which examined immunohistochemical changes of mucin- and serotonin-releasing cells in the small intestine of quails. The one-day-old quails included in the study were divided into five groups and fed with basal diet, prebiotics/probiotics, organic acids, a combination of prebiotics/probiotics and organic acids and antibiotic. Findings revealed an increase in the density of goblet cells in ileum in all supplemented groups except for those fed with antibiotic. Results also showed a decline in the number of intestinal serotonin positive cells in all birds, with cell depletion being lowest in those supplemented with organic acids.

The aim of this study was to evaluate clinical presentation and mortality rates, post mortem findings, histopathological and immunohistochemical patterns, route of contamination and prophylaxis of peste des petits ruminants virus (PPRV) infection in a flock of 525 sheep. On clinical examination a high body temperature, coughing, lachrymal and nasal discharge, stomatitis aphtosa and diarrhea were present. Gross post mortem examination revealed ulcerative stomatitis, interstitial pneumonia and acute catarrhal enteritis. Inclusion bodies were observed in labial tissues on histopathological investigation. Cells of the lung, intestine and labial tissues containing PPR viral antigens were observed using immunohistochemical technique by the streptavidin-biotin-peroxidase method. Mortality rate in suckling lambs was much higher (100%) than in young (24%) and adult (5%) sheep. [ABSTRACT FROM AUTHOR]

Infections of avian haemosporidian parasites are regularly identified by molecular methods including multiplex PCR, which allows researchers to distinguish mixed infections of parasites from multiple genera. Here we extend the utility of a previously designed multiplex PCR by designing a primer set specific to parasites of the subgenus Haemoproteus (genus: Haemoproteus). The updated one-step multiplex PCR protocol we describe here allows for the detection of the genera Plasmodium and Leucocytozoon and the two subgenera (Haemoproteus and Parahaemoproteus) of the genus Haemoproteus. A sensitivity analysis showed that the multiplex PCR could amplify DNA of parasites in the subgenus Haemoproteus at very low levels of infection. We used this multiplex PCR to identify haemosporidian infections in 250 adult domestic pigeons (Columba livia) in Turkey. All samples were also screened by microscopy and a widely used nested PCR to compare with the results of multiplex PCR, to detect low levels of parasitemia, and to identify possible abortive infections. In total, 71 pigeons (28.4%) were found to be infected by all three methods. The multiplex PCR protocol successfully detected and discriminated both subgenera Haemoproteus and Parahaemoproteus infections. We compared our results with previous host species records to assess the host specificity of the parasite lineages we found. Our findings provide novel data on the prevalence of avian haemosporidians in domestic pigeons and demonstrate the utility of the new one-step multiplex PCR protocol for the determination of mixed avian haemosporidian infections. We expect that this protocol will contribute to a better understanding of the distribution, epizootiology, and ecology of avian haemosporidians., (© 2022. The Author(s), under exclusive licence to Springer Nature B.V.)

Chronic burn pain persists for 6 months or longer after the wound has healed, affecting the patient's quality of life and daily activities. Pharmacological treatments are essential in the treatment of chronic pain after burns, but interventional methods come to the fore in cases where there is no response to pharmacological treatments. Radiofrequency ablation is known as a safe, effective, and minimally invasive treatment for patients with intractable chronic pain. Various pain syndromes can be successfully treated with radiofrequency ablation however, there is no publication of chronic pain management associated with burn injury. Here, we first describe the successful management of chronic burn pain using radiofrequency ablation of the medial antebrachial cutaneous nerve., (© The Author(s) 2023. Published by Oxford University Press on behalf of the American Burn Association. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

It was aimed to detect extracellular traps structures from sheep polymorphonuclear leukocytes (PMN) after being confronted with Echinococcus granulosus protoscoleces in vitro. Also, the effect of cyst fluid was examined on the development of extracellular traps. At the end of the incubation for 1 h, the extracellular traps augmented with neutrophil elastase, histone (H3) and myeloperoxidase were visualized in the protoscoleces-PMN co-culture microscopically. Some protoscoleces lysed and the chitinous hooks released were surrounded by the extracellular traps. The other protoscoleces were still intact and the extracellular trap structures were observed around them. The relationship between the extracellular DNA contents and the protoscoleces concentration was not found statistically significant (P > 0.05). The extracellular DNA amount in the co-cultures diluted in RPMI-1640 increased with the incubation time (P < 0.05). However, the time-dependent relationship was not found in the co-cultures diluted in the cyst fluid (P > 0.05). The difference in the extracellular DNA amount was detected as statistically significant (P < 0.05) between the two co-culture groups (diluted in RPMI-1640 or the cyst fluid), except for 30 min incubation. To the Author's knowledge, NETosis reaction was firstly observed in sheep PMN after being confronted with protoscoleces in vitro. The cyst fluid had some negative effects on the development of extracellular traps from sheep PMNs at the 1-h incubation time. It should be investigated which molecules are responsible for NETosis inhibition in hydatid cyst fluid. Future studies may clarify whether neutrophils fight with protoscoleces by using their different mechanisms., (Copyright © 2022 Elsevier Inc. All rights reserved.)

The aim of this study is to investigate the effect of different restoration methods applied to model teeth with a C-shaped root canal configuration on fracture strength. A total of 76 artificial tooth models were made using images of a molar tooth with a C-shaped root canal anatomy. The teeth were randomly divided into seven groups and different restorations, such as resin composite (2), bundled fiber post and resin composite (3), vertical fiber post and resin composite (4), horizontal fiber post and resin composite (5), woven fiber post and resin composite (6) and composite endocrown (7) were applied to the different groups except control group (1). The artificial teeth were embedded in acrylic blocks and subjected to fracture tests. The data were analyzed using one-way analysis of variance, Monte Carlo Pearson Chi-square, Pearson Chi-square, and Fisher's exact test (P < 0.05). All groups differed in terms of fracture strength (P = 0.001). The highest fracture strength observed in group 6, and there was a significant difference between group 6 and group 4 based on a least significant difference pairwise comparison test. In terms of fracture type, the highest reparability percentage (100%) observed in group 7. The fracture strength values of endocrown restorations and woven fiber-reinforced resin composite restorations were found to be as high as those of the intact models. Considering also fracture restorability, endocrown restorations may be recommended for teeth with C-shaped root canal anatomy. The usage of the 3D tooth models in the studies offer a valuable opportunity in terms of the standardization of the samples, particularly in teeth with anatomical variations. This study shows that using of this technology, homogeneous groups can be created and experimental studies can be improved., (© 2021. The Society of The Nippon Dental University.)

Introduction: In preeclampsia (PE), human decidua mesenchymal stromal cells (hDMSCs) are exposed to abnormally high levels of oxidative stress and inflammatory factors circulating in the maternal blood. MicroRNAs (miRNAs) have been shown to have a significant impact on the differentiation, maturation and function of mesenchymal stromal cells (MSCs). Our aim in the present study is firstly to investigate differentially expressed miRNA levels to be used as a biomarker in the early detection of PE and secondly to investigate whether those differentially expressed miRNAs in hDMSCs have an effect on the pathogenesis of PE., Methods: This study covers miRNA expression analysis of hDMSCs from 7 PE patient and 7 healthy pregnant women and is a preliminary study to investigate putative biomarkers. After cell culture and cell sorting, total RNA including miRNAs were isolated from hDMSCs. Let-7b-3p, let-7f-1-3p, miR-191-3p, miR-550a-5p, miR-33b-3p and miR-425-3p were used for miRNA analysis and U6 snRNA was used for normalization of the samples. MiRNA analysis was performed by droplet digital polymerase chain reaction (ddPCR) method and obtained results were evaluated statistically., Results: As a result of the analysis, it was observed that the levels of hsa-miR-33b-3p significantly (AUC: 0.93, p = 0.04, fold change: 4.5) increased in hDMSC of PE patients compared to healthy controls. However, let-7b-3p, let-7f-1-3p, miR-191-3p, miR-550a-5p, and miR-425-3p were not considered as significant because they did not meet the p < 0,05 requirement., Discussion: Within the scope of the study, it is predicted that miR-33b-3p (p = 0.004, AUC = 0.93) can be used as a biomarker in detecting PE., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Aim: Sexual health needs are one of the holistic care needs. Nurses need to help individuals identify and meet these needs. However, sometimes their perception of sexuality can affect their attitudes towards care. The aim of this study determine the effect of sexual self-schema and sexual embarrassment on attitudes and beliefs related to sexual health in nursing students., Background: The sexual self-schema, which emphasizes the cognitive aspect of sexuality, is defined as cognitive generalizations that a person does for her/his own sexuality. Sexual self-schemas are known to affect individuals' feelings, beliefs and attitudes towards sexuality. Those with a positive sexual self-schema experience less embarrassment and more positive attitudes towards sexuality. Those with a negative sexual self-schema experience more sexual embarrassment. Those who experience sexual embarrassment have an avoidance of sexual interaction and talking about sexual issues. Nurses and student nurses have embarrassed to talk about sexuality with their patients. It is thought that sexual attitudes and beliefs are effective on this situation., Design: This study was conducted in a cross-sectional descriptive type. Structural equality model was established to test the hypotheses of the research. In the model, the direct effects of the sexual self-schema and the sexual embarrassment on sexual attitudes and beliefs were examined. In addition, the mediating effect of sexual embarrassment between sexual schemas and sexual attitudes and beliefs is also included in the model., Methods: The study population was the students who attended to and conducted clinical practice at the second, third and fourth study year at a Faculty of Health Sciences. For data collection, the Introductory Information Form which questioned socio-demographic characteristics of individuals, Sexual Self-Schema Scale (SSS), Sexual Embarrassment Scale (SES), and Sexual Attitudes and Beliefs Survey for Sexual Health (SABS) were used. The data were collected online with voluntary participation., Results: Students' average scale scores were: SSS: 121.57 ± 21.23, SES: 12.16 ± 5.74 and SABS: 35.62 ± 7.53. It was also found that sexual self-schemas and sexual embarrassment effected sexual attitudes and beliefs., Conclusion: It could be recommended that these results be tested with different studies, that the course content on sexual issues be reviewed be addressed in a broader context., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Introduction: This study aimed to evaluate the effect of calcium hydroxide and bioceramics used in perforation repair on the bonding strength of fiber posts via a push-out test., Methodology: This study used 106 extracted single-rooted human mandibular premolar teeth. Root canal preparations were performed with a rotary file system and perforations were created in the middle third of each tooth. The samples were randomized into two main experimental groups, one with calcium hydroxide and one without. Each group had four subgroups in which different bioceramic cements were applied ( n  = 11) and a control group ( n  = 9). The root canals perforations were repaired using MTA, Biodentine, Bioaggregate, and Endosequence BC root repair material. A fiber post was applied to each tooth and a push-out test was performed. The samples were examined at 40× magnification with a digital microscope in order to identify fracture type., Results: Bonding strength was calculated in MPa. A statistical analysis showed that the calcium hydroxide had no effect on the bonding strength of the fiber posts. A comparison of the perforation repair materials revealed that Biodentine in the calcium hydroxide group and Bioaggregate in both groups decreased the bonding strength compared to the other materials ( p  < 0.05). The most common failure type was adhesive failure between the dentin and resin cement (38.16%)., Conclusions: The use of different perforation repair materials can affect the bonding strength of fiber posts. Therefore, the choice of perforation repair material should be made on an individual basis.

The aim of this study was to evaluate the physical properties of calcium-silicate based cements as retrograde filling in different pH and blood conditions using micro-CT. Eighty-four teeth were obturated and after root-end resection, 3 mm-deep root-end cavity was ultrasonically prepared. The samples were divided into four test groups (n=21). Cements were freshly prepared and analyzed with micro-CT before and after exposed. The test groups were exposed for four days to environments containing acid, alkali, or blood. An acidic pH significantly reduced the volume of all materials after four days and differed from the other environments. Biodentine has the highest percentage of loss in volume and density after treatment in an acidic environment. Porosity formation in the acidic medium was observed in Biodentine amongst all materials. The three-dimensional structures of all materials changed after exposed to an acidic pH, while fewer changes were observed in the structures of materials treated with blood and alkali.

This study examined the relationship between sexual dysfunction and marital adjustment in women with hypertension and the effect of the cultures on the sexual problems of women. Mixed method was used in this study. The study was conducted as quantitative (in descriptive type) to examine sexual dysfunction and marital adjustment in women with hypertension and as qualitative to examine the effect of culture on the views of individuals on sexual problems. The study included 102 women who met inclusion criteria (who were on medication and had active sexual life for 1 year). Patient information form, Arizona Sexual Experience Scale (Female), and Marital Adjustment Test (MAT) were used to collect the data. The mean age of the subjects was found to be 55.10 ± 9.13, the mean duration of hypertension diagnosis was 8.66 ± 6.43, and the mean time to start was 7.87 ± 6.30. The mean duration of marriage for the women with hypertension was 34.08 ± 12.507. There was a negative correlation between sexual orientation, orgasm and sexual satisfaction from the subscales of ASEX and the overall scale (p < 0.05). Accordingly, as the adaptation in marriage diminished, sexual problems increased in women. As a result of the study, it was determined that HT had a negative effect on sexual functioning problems and marital adjustment, but women accepted this situation because of their culture, "women do not have the right to sexuality" and naturally accept the absence of sexuality after a certain age.

Automated external defibrillators are pervasive computing devices designed for the treatment and management of acute sudden cardiac arrest. This study aims to explain users' actual use behavior in teams formed by different professions taken after a short time span of interaction with automated external defibrillator. Before the intervention, all the participants were certified with the American Heart Association Basic Life Support for healthcare providers. A statistically significant difference was revealed in mean individual automated external defibrillator technical skills between uniprofessional and interprofessional groups. The technical automated external defibrillator team scores were greater for groups with interprofessional than for those with uniprofessional education. The nontechnical automated external defibrillator skills of interprofessional and uniprofessional teams revealed differences in advantage of interprofessional teams. Students positively accept automated external defibrillators if well-defined and validated training opportunities to use them expertly are available. Uniprofessional teams were successfully supported by their members and, thereby, used automated external defibrillator effectively. Furthermore, the interprofessional approach resulted in as much effective teamwork as the uniprofessional approach.

This study was carried out to assess the effects of reminiscence therapy that was supported with internet-based videos on the cognitive condition and apathy levels of older people with mild dementia living in nursing homes. This randomized controlled experimental study was conducted between May 15, 2016, and August 25, 2016, in two nursing homes in Adana, Turkey. A total of 32 people participated in this study, 16 individuals were in the intervention group and 16 individuals were in the control group. The data were collected using a Personal Information Form, the Standardized Mini-Mental State Examination (SMMSE) test, and the Apathy Rating Scale (ARS) Self-assessment Form. The individuals in the intervention group received group reminiscence therapy involving internet-based videos for 60 minutes once a week for 3 months. During this period, 25 to 30 minutes of unstructured interviews were carried out with individuals in the control group. The data were analyzed using descriptive statistics, Student t test, the Mann-Whitney U test, and Wilcoxon signed rank test. In this study, a significant difference was found between experimental and control groups' posttest mean scores on the SMMSE test and ARS ( P < .01). The experimental group's SMMSE test and ARS posttest mean scores were higher than those of the control group ( P < .01). This study found that group reminiscence therapy using internet-based videos improved the cognitive functions and apathy levels of older people with mild dementia of the Alzheimer type. It is recommended that group reminiscence therapy studies with Internet-based videos be conducted in care institutions that are responsible for managing older people with mild dementia of the Alzheimer type.

Aim: The objective of this study was to determine the neuroprotective effects of 2-aminoethyl diphenyl-borinate (2-APB) on the brains of rats with experimentally-induced severe acute pancreatitis., Materials and Methods: Thirty Spraque-Dawley male rats with an average weight of 200-250 grams were randomly divided into three groups. Group 1: Sham group, Group 2: Severe acute pancreatitis group, Group 3: Treatment group with severe acute pancreatitis, given 2 mg/kg 2-APB before pancreatitis onset. In Groups 2 and 3, severe acute pancreatitis was induced by intraperitoneal administration of 1.5 g/kg L-arginine with a 1-hour interval. Tumor necrosis factor-α, interleukin 6, pancreatic amylase were all measured. Brain tissue samples were evaluated histopathologically. TUNEL staining method was used to visualize apoptotic cells., Results: In Group 3, it was determined that the density of TUNEL-positive cells in the cerebral cortex has decreased, while the number of Bcl-2-positive cells had increased. In Group 3, it was observed that glial aggregation areas were diminished and histopathological changes were decreased as compared to Group 2. In Group 2, on the other hand, it was observed that in areas with glial cell aggregation, the density of TUNEL-positive glial cells had increased, while Bcl-2-positive cell reaction has been feeble., Conclusions: It was observed that 2-APB decreases neuronal apoptosis and glial cell aggregation (Tab. 2, Fig. 3, Ref. 21).

Cardiopulmonary resuscitation training is an essential element of clinical skill development for healthcare providers. The International Liaison Committee on Resuscitation has described issues related to cardiopulmonary resuscitation and emergency cardiovascular care education. Educational interventions have been initiated to try to address these issues using a team-based approach and simulation technologies that offer a controlled, safe learning environment. The aim of the study is to review and synthesize published studies that address the primary question "What are the features and effectiveness of educational interventions related to simulation-enhanced, team-based cardiopulmonary resuscitation training?" We conducted a systematic review focused on educational interventions pertaining to cardiac arrest and emergencies that addressed this main question. The findings are presented together with a discussion of the effectiveness of various educational interventions. In conclusion, student attitudes toward interprofessional learning and simulation experiences were more positive. Research reports emphasized the importance of adherence to established guidelines, adopting a holistic approach to training, and that preliminary training, briefing, deliberate practices, and debriefing should help to overcome deficiencies in cardiopulmonary resuscitation training., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

In this study, we investigated the protective effect of selenium (Se) on cisplatin (Cis) induced testicular damage using histopathological, immunohistochemical and biochemical approaches. Twenty-one male Wistar rats were equally divided into three groups of seven rats each: control (C), Cis, and Cis+Se. Cis and Cis+Se group rats received Cis at a dose of 12mg/kg b.w./day, intraperitoneally for 3 consecutive days. Cis+Se group rats received selenium via oral gavage 3mg/kg/day (twice-a day as 1.5mg/kg) until 11th consecutive days starting at 5 days before cisplatin injection. C group received only 0.9% NaCl intraperitoneally and orally at same time and at equal volume. After the treatment, the histopathological, immunohistochemical and biochemical examinations were performed. In seminiferous tubules of Cis treated rats were observed the most consistent findings characterized with vacuolization, desquamation, disorganization, and also was a considerable reduction in elongated spermatids, however the Cis+Se group exhibited improved histopathologic changes. In the immunohistochemical examinations, caspase-3 immunopositive cells displayed higher in the Cis group according to C and Cis+Se groups. Bcl-2 and NF-κB staining revealed a moderate number in the C group and significantly fewer in the Cis group compared to the Cis+Se groups. Additionally, MDA levels were also significantly increased in the Cis group in comparison to Control group, but pretreatment with selenium prevented elevation of MDA levels significantly in Cis+Se group rats. This study indicates that Cis-treatment induced testicular apoptosis and lipid peroxidation, and combined treatment with selenium prevented severity of the toxicity in rats., (Copyright © 2016 Elsevier GmbH. All rights reserved.)

To investigate the levels of seven elements using an inductively coupled plasma-mass spectrometry (ICP-MS) method in rat organs after the implantation of Micro Mega Mineral Trioxide Aggregate (MM-MTA), Bioaggregate (BA) and Biodentine (BD) materials. MM-MTA, BA and BD were implanted into the subcutaneous tissue of 15 Wistar albino rats; three control animals had no operation. After 45 days, the rats were sacrificed and their brains, kidneys and livers were removed. The ICP-MS analysis was used to determine trace elements. Data were analysed using the Kruskal-Wallis and Connover post hoc tests. There was no significant difference between the control groups and the MM-MTA, BA and BD groups according to the concentration of aluminum, calcium, arsenic and lead in the rats' organs. Beryllium was not detected in all tissue samples. Chromium levels of these materials were higher than the control group in brain and kidney samples (P = 0.038 and P = 0.037); magnesium levels were higher than the control group in kidney and liver samples (P = 0.030 and P = 0.008). MM-MTA, BA and BD were nontoxic according to trace element levels in brain, kidney and liver samples of rats. Further investigation is required to understand the systemic effects of these materials.

Background: Chemotherapeutic agents may lead to serious neurological side effects, which in turn can deteriorate the quality of life and cause dose limiting. Direct toxic effect or metabolic derangement of chemotherapeutic agents may cause these complications. Cabazitaxel is a next generation semi-synthetic taxane derivative, which is effective in both preclinical models of human tumors sensitive or resistant to chemotherapy and in patients with progressive prostate cancer despite docetaxel treatment., Aim: The primary aim of this study was to investigate the central nervous system toxicity of Cabazitaxel. Secondary aim was to investigate the safety dose of Cabazitaxel for the central nervous system., Methods: A total of 24 adult male Wistar-Albino rats were equally and randomly divided into four groups as follows: group 1 (Controls), group 2 (Cabazitaxel 0.5mg/kg), group 3 (Cabazitaxel 1.0mg/kg) and group 4 (Cabazitaxel 1.5mg/kg). Cabazitaxel (Jevtana, Sanofi-Aventis USA) was intraperitoneally administered to groups 2, 3 and 4 at 0.5, 1.0 and 1.5mg/kg (body-weight/week) doses, respectively for four consecutive weeks. Beside this, group 1 received only i.p. saline at the same volume and time. At the end of the study, animals were sacrificed and bilateral brain hemispheres were removed for biochemical, histopathological and immunohistochemical examinations., Results: Intraperitoneal administration of Cabazitaxel has exerted neurotoxic effect on rat brain. We have observed that biochemical and immunohistochemical results became worse in a dose dependent manner., Conclusion: Our findings have suggested that Cabazitaxel may be a neurotoxic agent and can trigger apoptosis in neuron cells especially at high doses., (Copyright © 2015 Elsevier B.V. All rights reserved.)

Purpose: To evaluate the central nervous system toxicity of cisplatin and neuroprotective effect of selenium., Methods: Twenty-one male Wistar albino rats were divided into three groups: control (C), cisplatin (CS), cisplatin and selenium (CSE, n=7 in each group). Cisplatin (12 mg/kg/day, i.p.) was administered to CS and CSE groups for three days. Furthermore, CSE group received 3mg/kg/day (twice-a-day as 1.5 mg/kg) selenium via oral gavage five days before cisplatin injection and continued for 11 consecutive days. The same volumes of saline were administered to C group intraperitoneally and orally at same time., Results: Heterochromatic and vacuolated neurons and dilated capillary vessels in the brain were observed in the histochemical examinations of cisplatin treated group. Rats that were given a dose of 3mg/kg/day selenium decreased the cisplatin induced histopathological changes in the brain, indicating a protective effect. In addition, cytoplasmic staining of the cell for bcl-2, both cytoplasmic and nuclear staining for bax were determined to be positive in the all groups. Bax positive cells were increased in the CS group compared to C group, in contrast to decreased bcl-2 positivity., Conclusion: Selenium limited apototic activity and histological changes due to the cisplatin related central neurotoxicity.

This study was aimed to use micro-computed tomography (μ-CT) to evaluate the canal shaping properties of three nickel-titanium instruments, Self-Adjusting File (SAF), Reciproc, and Revo-S rotary file, in maxillary first molars. Thirty maxillary molars were scanned preoperatively by using micro-computed tomography (μ-CT) scans at 13,68 μm resolution. The teeth were randomly assigned to three groups (n = 10). The root canals were shaped with SAF, Reciproc, and Revo-S, respectively. The shaped root canals were rescanned. Changes in canal volumes and surface areas were compared with preoperative values. The data were analyzed using Kruskal-Wallis and Conover's post hoc tests, with p < .05 denoting a statistically significant difference. Preoperatively canal volumes and surface area were statistically similar among the three groups (p > .05). There were statistically significant differences in all measures comparing preoperative and postoperative canal models (p = 0.0001). These differences occurred after instrumentation among the three experimental groups showed no statistically significant difference for volume (p > .05). Surface area showed the similar activity in buccal canals in each of the three techniques whereas no statistically significant difference was detected among surface area, the SAF, and the Revo-S in the palatal (P) canal. Each of three shaping system showed the similar volume activity in all canals, but SAF and Revo-S provided more effectively root planning in comparison with Reciproc in P canal., (© Wiley Periodicals, Inc.)

Objective: The objective was to evaluate the subcutaneous biocompatibility of two root canal sealers., Materials and Methods: Thirty female rats were distributed into two groups of 15 animals. Each received subcutaneous dorsal implants: Silicone tubes filled with AH Plus or Obtuseal. After the 11(th), 14(th), and 45(th) days, tissues were collected for biopsy and fixed and processed for histologic evaluation. Observations of the cellular inflammatory components, such as lymphocytes and macrophages, were made. The data were analyzed using Kruskal-Wallis and Connover tests (P < 0.05)., Results: By the 7(th) day, lymphocyte infiltration increased in both groups (P = 0.0104). However, macrophage infiltration increased only in the AH Plus group (P = 0.0011). By the 14(th) and 45(th) days, lymphocyte and macrophage infiltrations were reduced. At the end of the experimental period, no statistically significant differences were found between the AH Plus group and the Obtuseal group (P > 0.05)., Conclusion: After 45 days, Obtuseal yielded a satisfactory tissue reaction; it was biocompatible when tested in subcutaneous rat tissue. On the basis of the obtained results, it was concluded that Obtuseal root canal sealer caused a satisfactory tissue reaction as AH plus, because it was biocompatible when tested in the subcutaneous tissue of rats.

Purpose: This study was descriptively conducted to explore attitudes of nursing students related to caring for dying patients in central Anatolia., Methods: The study was completed with 222 third-year students of nursing school originating from three universities in Turkey., Result: It was explored that Frommelt Attitudes Toward Care of the Dying Scale (FATCOD) scores of the students who had previously cared for terminal patients at the clinics were significantly higher, and the scores of those who regarded themselves as nonbeliever in terms of religion were significantly lower (p < .05)., Conclusion: It was seen that nursing students' FATCOD scores were at a moderate level., Implications for Nursing Practice: We are of the opinion that it will be helpful that nursing students should be trained about the care for dying patients during their formal education., (© 2014 NANDA International, Inc.)