280 results on '"Simopoulou, Mara"'
Search Results
2. Systemic inflammatory markers following adjunctive Nd:YAG (1064 nm) laser irradiation to step 2 of periodontal therapy: a 12-month, randomized, controlled trial
- Author
-
Markou, Nikolaos, Pepelassi, Eudoxie, Madianos, Phoebus, Simopoulou, Mara, and Karoussis, Ioannis K.
- Published
- 2023
- Full Text
- View/download PDF
3. Correction to: Systemic inflammatory markers following adjunctive Nd:YAG (1064 nm) laser irradiation to step 2 of periodontal therapy: a 12‑month, randomized, controlled trial
- Author
-
Markou, Nikolaos, Pepelassi, Eudoxie, Madianos, Phoebus, Simopoulou, Mara, and Karoussis, Ioannis K.
- Published
- 2023
- Full Text
- View/download PDF
4. Development of a predictive model for luteal phase oocyte retrieval in poor responders undergoing natural cycle IVF
- Author
-
Simopoulou, Mara, Galatis, Dionysios, Maziotis, Evangelos, Pantou, Agni, Giannelou, Polina, Grigoriadis, Sokratis, Tzonis, Panagiotis, Griva, Theodora, Zikopoulos, Athanasios, Philippou, Anastasios, Koutsilieris, Michael, Pantos, Konstantinos, and Sfakianoudis, Konstantinos
- Published
- 2022
- Full Text
- View/download PDF
5. Impact of Hysteroscopic Polypectomy on IVF Outcomes in Women with Unexplained Infertility.
- Author
-
Triantafyllidou, Olga, Korompokis, Ilias, Chasiakou, Stamatia, Bakas, Panagiotis, Kalampokas, Theodoros, Simopoulou, Mara, Tzanakaki, Despoina, Kalampokas, Emmanouil, Panagodimou, Evangelia, Xepapadaki, Maria, Christopoulos, Panagiotis, Valsamakis, Georgios, and Vlahos, Nikolaos F
- Subjects
HYSTEROSCOPIC surgery ,EMBRYO implantation ,HUMAN in vitro fertilization ,WOMEN'S hospitals ,POLYPECTOMY ,FERTILIZATION in vitro ,INDUCED ovulation - Abstract
Objective: To assess the effect of hysteroscopic polypectomy on the in vitro fertilization (IVF) results in infertile women with at least one prior negative IVF outcome. Methods: This retrospective cohort study included women who had attended the "2nd Department of Obstetrics and Gynecology of the National and Kapodistrian University of Athens" and "Iaso" Maternity Hospital from October 2019 to January 2023 for infertility treatment. The medical records of 345 women aged 18–45 years old without abnormal findings in hysterosalpingography (HSG) and with at least one previous failed IVF procedure were analyzed. The male factor was excluded, as well as a prior hysteroscopic removal of polyps. In 67 women, polyps were suspected during initial two-dimensional ultrasound (2D-US) examination. The final sample of the study comprised 40 patients, in which endometrial polyps were removed by hysteroscopy with the use of resectoscope. All patients underwent ovarian stimulation and IVF in the consecutive cycle using a short GnRh antagonist protocol. Main Results: After hysteroscopic polypectomy, 29 (72.5%) out of 40 patients had a positive pregnancy result: 26 (65%) clinical and 3 (7.5%) biochemical pregnancies were documented. There was a statistically significant difference between the number of clinical pregnancies before and after polypectomy (p < 0.001), as well as between the total number of pregnancies (p < 0.001). Secondary Results: Women with positive outcome were significantly younger and had significantly lower FSH levels (p < 0.007). They also had significantly higher AMH (p < 0.009) and peak estradiol levels (p < 0.013) and yielded more M II oocytes (p < 0.009) and embryos (p < 0.002). Conclusions: Hysteroscopic polypectomy in women with a suspected endometrial polyp using 2D ultrasound and a history of prior failed IVF attempt improves IVF outcomes in terms of the clinical and total number of pregnancies. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
6. Correction to: Assessing the practice of LuPOR for poor responders: a prospective study evaluating follicular fluid cfDNA levels during natural IVF cycles
- Author
-
Sfakianoudis, Konstantinos, Tsioulou, Petroula, Maziotis, Evangelos, Giannelou, Polina, Glava, Argyro, Grigoriadis, Sokratis, Rapani, Anna, Nezos, Andrianos, Pantou, Agni, Koutsilieris, Michael, Pantos, Konstantinos, Mastorakos, George, and Simopoulou, Mara
- Published
- 2023
- Full Text
- View/download PDF
7. PGT-A: who and when? Α systematic review and network meta-analysis of RCTs
- Author
-
Simopoulou, Mara, Sfakianoudis, Konstantinos, Maziotis, Evangelos, Tsioulou, Petroula, Grigoriadis, Sokratis, Rapani, Anna, Giannelou, Polina, Asimakopoulou, Marilena, Kokkali, Georgia, Pantou, Amelia, Nikolettos, Konstantinos, Vlahos, Nikolaos, and Pantos, Konstantinos
- Published
- 2021
- Full Text
- View/download PDF
8. The Role of One-Carbon Metabolism and Methyl Donors in Medically Assisted Reproduction: A Narrative Review of the Literature.
- Author
-
Sfakianoudis, Konstantinos, Zikopoulos, Athanasios, Grigoriadis, Sokratis, Seretis, Nikolaos, Maziotis, Evangelos, Anifandis, George, Xystra, Paraskevi, Kostoulas, Charilaos, Giougli, Urania, Pantos, Konstantinos, Simopoulou, Mara, and Georgiou, Ioannis
- Subjects
REPRODUCTIVE technology ,LITERATURE reviews ,FERTILIZATION in vitro ,METABOLISM ,VITAMIN B12 ,FOLIC acid ,ZINC supplements - Abstract
One-carbon (1-C) metabolic deficiency impairs homeostasis, driving disease development, including infertility. It is of importance to summarize the current evidence regarding the clinical utility of 1-C metabolism-related biomolecules and methyl donors, namely, folate, betaine, choline, vitamin B12, homocysteine (Hcy), and zinc, as potential biomarkers, dietary supplements, and culture media supplements in the context of medically assisted reproduction (MAR). A narrative review of the literature was conducted in the PubMed/Medline database. Diet, ageing, and the endocrine milieu of individuals affect both 1-C metabolism and fertility status. In vitro fertilization (IVF) techniques, and culture conditions in particular, have a direct impact on 1-C metabolic activity in gametes and embryos. Critical analysis indicated that zinc supplementation in cryopreservation media may be a promising approach to reducing oxidative damage, while female serum homocysteine levels may be employed as a possible biomarker for predicting IVF outcomes. Nonetheless, the level of evidence is low, and future studies are needed to verify these data. One-carbon metabolism-related processes, including redox defense and epigenetic regulation, may be compromised in IVF-derived embryos. The study of 1-C metabolism may lead the way towards improving MAR efficiency and safety and ensuring the lifelong health of MAR infants. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
9. The Effect of Open and Closed Oocyte Vitrification Systems on Embryo Development: A Systematic Review and Network Meta-Analysis.
- Author
-
Pantos, Konstantinos, Maziotis, Evangelos, Trypidi, Anna, Grigoriadis, Sokratis, Agapitou, Kristi, Pantou, Agni, Nikolettos, Konstantinos, Kokkini, Georgia, Sfakianoudis, Konstantinos, Pomeroy, Kimball O., and Simopoulou, Mara
- Subjects
VITRIFICATION ,OVUM ,FERTILITY preservation ,BLASTOCYST ,SURVIVAL rate - Abstract
Background/Objectives: Open and closed vitrification systems are commonly employed in oocyte cryopreservation; however, there is limited evidence regarding a comparison of their separate impact on oocyte competence. This study uniquely brings to the literature, data on the effect of open versus closed vitrification systems on laboratory and clinical outcomes, and the effect of cooling and warming rates. Methods: A systematic search of the literature was performed using the databases PubMed/MEDLINE and the Cochrane Central Library, limited to articles published in English up to January 2023. A network meta-analysis was conducted comparing each vitrification system versus fresh oocytes. Results: Twenty-three studies were included. When compared to fresh oocytes, both vitrification devices resulted in lower fertilization rates per MII oocyte retrieved. When comparing the two systems in terms of survival rates, no statistically significant difference was observed. However, interestingly open systems resulted in lower cleavage and blastocyst formation rates per 2 pronuclear (2PN) oocyte compared to fresh controls, while at the same time no statistically significant difference was detected when comparing closed devices with fresh oocytes. Conclusions: In conclusion, closed vitrification systems appear to exert a less detrimental impact on the oocytes' competence, which is reflected in the blastocyst formation rates. Proof of superiority of one system versus the other may lead to standardization, helping to ultimately determine optimal practice in oocyte vitrification. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
10. Navigating assisted reproduction treatment in the time of COVID-19: concerns and considerations
- Author
-
Simopoulou, Mara, Sfakianoudis, Konstantinos, Giannelou, Polina, Rapani, Anna, Siristatidis, Charalampos, Bakas, Panagiotis, Vlahos, Nikolaos, and Pantos, Konstantinos
- Published
- 2020
- Full Text
- View/download PDF
11. What is the true place of a double stimulation and double oocyte retrieval in the same cycle for patients diagnosed with poor ovarian reserve? A systematic review including a meta-analytical approach
- Author
-
Sfakianoudis, Konstantinos, Pantos, Konstantinos, Grigoriadis, Sokratis, Rapani, Anna, Maziotis, Evangelos, Tsioulou, Petroula, Giannelou, Polina, Kontogeorgi, Adamantia, Pantou, Agni, Vlahos, Nikolaos, Koutsilieris, Michael, and Simopoulou, Mara
- Published
- 2020
- Full Text
- View/download PDF
12. What will the future hold for artificial organs in the service of assisted reproduction: prospects and considerations
- Author
-
Simopoulou, Mara, Sfakianoudis, Konstantinos, Tsioulou, Petroula, Rapani, Anna, Giannelou, Polina, Kiriakopoulos, Nikolaos, Pantou, Agni, Vlahos, Nikolaos, Anifandis, George, Bolaris, Stamatis, Pantos, Konstantinos, and Koutsilieris, Michael
- Published
- 2019
- Full Text
- View/download PDF
13. Embryo Transfer Procedural Parameters Do Not Predict IVF Cycle Outcome.
- Author
-
Sfakianoudis, Konstantinos, Maziotis, Evangelos, Trypidi, Anna, Grigoriadis, Sokratis, Vaxevanoglou, Terpsithea, Angeli, Irene, Rapani, Anna, Kotsifaki, Amalia, Pistola, Kalliopi, Pantou, Agni, Dafopoulos, Konstantinos, Pantos, Konstantinos, and Simopoulou, Mara
- Subjects
EMBRYO transfer ,FERTILIZATION in vitro ,CYCLING ,MATERNAL age ,STATISTICAL significance - Abstract
Background: this study aims to assess the effect of embryo transfer (ET) performance parameters of a technical nature on IVF outcome. Methods: A total of 1417 ETs from a single IVF center were included in this prospective observational study. The parameters investigated were as follows: the presence of cervical mucus post catheter withdrawal, the presence of blood, catheter reload, the employment of a tenaculum and stylet, catheter resistance as experienced by the physician and patient discomfort. Results: When ET performance parameters were associated with clinical outcomes on a singular level, none of the ET parameters presented with any statistical significance. The evaluation of covariates indicated that the number and the quality of transferred embryos, as well as maternal age, exerted a statistically significant effect on clinical outcomes. In a multivariate analysis, only the presence of mucus along with significant catheter resistance presented with statistical significance; however, when adjusting for covariates, this combination showed no statistically significant effect on clinical outcomes. Conclusions: the results indicate that the time-consuming process of recording and analyzing ET performance parameters fails to offer any additional value in predicting the cycle's outcome, while factors like embryo quality and number, as well as maternal age, seem to be the sole robust predictive factors of an IVF cycle. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
14. Treating infertility: current affairs of cross-border reproductive care
- Author
-
Simopoulou Mara, Sfakianoudis Konstantinos, Giannelou Polina, Pierouli Aikaterini, Rapani Anna, Maziotis Evangelos, Galatis Dionysios, Bakas Panagiotis, Vlahos Nikolaos, Pantos Konstantinos, and Koutsilieris Michael
- Subjects
cbrc ,risks ,bioethics ,art ,Medicine - Abstract
Infertility patients are willing to travel abroad to receive the medical treatment of choice. A 2010 study reported that approximately 25,000 couples travel abroad annually seeking infertility treatment. The purpose of this review is to analyze the criteria and risks related to cross-border reproductive care (CBRC) from the perspective of the patients and explore the issues raised regarding the country of origin and the destination country. A computerized search was performed in PubMed employing respective keywords. The total number of published articles provided by our PubMed search was 1905. Criteria for selecting the destination country include: the economic status, legislation, quality of care and anonymity. Despite the fact that CBRC is becoming a familiar concept, it raises concerns for the practitioner and issues of a social and bioethical nature. Most of them stem from the fact that health care acquires a commercialization aspect. Medical tourism entails several risks, such as misconceptions regarding the destination country, and legal issues arising from differences in the judiciary systems. Larger studies evaluating all aspects of CBRC are imperative. Quality assurance, a consensus and a common platform of practice, along with a system of international governance based on human rights, are a necessity for CBRC patients.
- Published
- 2019
- Full Text
- View/download PDF
15. Accuracy and efficacy of embryo transfer based on the previous measurement of cervical length and total uterine length
- Author
-
Bakas, Panagiotis, Simopoulou, Mara, Giner, Maria, Tzanakaki, Despina, and Deligeoroglou, Eythimios
- Published
- 2019
- Full Text
- View/download PDF
16. Evaluating the value of day 0 of an ICSI cycle on indicating laboratory outcome
- Author
-
Maziotis, E., Sfakianoudis, K., Giannelou, P., Grigoriadis, S., Rapani, A., Tsioulou, P., Nikolettos, K., Pantou, A., Tiptiri-Kourpeti, A., Koutsilieris, M., Asimakopoulos, B., Nikolettos, N., Pantos, K., and Simopoulou, Mara
- Published
- 2020
- Full Text
- View/download PDF
17. Couples with mild male factor infertility and at least 3 failed previous IVF attempts may benefit from laparoscopic investigation regarding assisted reproduction outcome
- Author
-
Pantou, Agni, Sfakianoudis, Konstantinos, Maziotis, Evangelos, Giannelou, Polina, Grigoriadis, Sokratis, Tsioulou, Petroula, Kokkali, Georgia, Koutsilieris, Michael, Pantos, Konstantinos, and Simopoulou, Mara
- Published
- 2020
- Full Text
- View/download PDF
18. Controversy and consensus on the management of elevated sperm DNA fragmentation in male infertility: A global survey, current guidelines, and expert recommendations
- Author
-
Farkouh, Ala’a, Agarwal, Ashok, Hamoda, Taha Abo-Almagd Abdel-Meguid, Kavoussi, Parviz, Saleh, Ramadan, Zini, Armand, Arafa, Mohamed, Harraz, Ahmed M., Gul, Murat, Karthikeyan, Vilvapathy Senguttuvan, Durairajanayagam, Damayanthi, Rambhatla, Amarnath, Boitrelle, Florence, Chung, Eric, Birowo, Ponco, Toprak, Tuncay, Ghayda, Ramy Abou, Cannarella, Rossella, Phuoc, Nguyen Ho Vinh, Dimitriadis, Fotios, Russo, Giorgio I., Sokolakis, Ioannis, Mostafa, Taymour, Makarounis, Konstantinos, Ziouziou, Imad, Kuroda, Shinnosuke, Bendayan, Marion, Kaiyal, Raneen Sawaid, Japari, Andrian, Simopoulou, Mara, Rocco, Lucia, Garrido, Nicolas, Gherabi, Nazim, Bocu, Kadir, Kahraman, Oguzhan, Le, Tan V., Wyns, Christine, Tremellen, Kelton, Sarikaya, Selcuk, Lewis, Sheena, Evenson, Donald P., Ko, Edmund, Calogero, Aldo E., Bahar, Fahmi, Martinez, Marlon, Crafa, Andrea, Nguyen, Quang, Ambar, Rafael F., Colpi, Giovanni, Bakircioglu, Mustafa Emre, Henkel, Ralf, Kandil, Hussein, Serefoglu, Ege Can, Alarbid, Abdullah, Tsujimura, Akira, Kheradmand, Alireza, Anagnostopoulou, Christina, Marino, Angelo, Adamyan, Aram, Zilaitiene, Birute, Ozer, Cevahir, Pescatori, Edoardo, Vogiatzi, Paraskevi, Busetto, Gian Maria, Balercia, Giancarlo, Elbardisi, Haitham, Akhavizadegan, Hamed, Sajadi, Hesamoddin, Taniguchi, Hisanori, Park, Hyun Jun, Rosas, Israel Maldonado, Al-Marhoon, Mohamed, Gilani, Mohammad Ali Sadighi, Alhathal, Naif, Pinggera, Germar-Michael, Kothari, Priyank, Mogharabian, Nasser, Micic, Sava, Homa, Sheryl T., Darbandi, Sara, Long, Tran Quang Tien, Zohdy, Wael, Atmoko, Widi, Sabbaghian, Marjan, Ibrahim, Wael, Smith, Ryan P., Ho, Christopher Chee Kong, Rosette, Jean de la, El-Sakka, Ahmed I., Preto, Mirko, Zenoaga-Barbăroșie, Cătălina, Abumelha, Saad Mohammed, Baser, Aykut, Aydos, Kaan, Ramirez-Dominguez, Liliana, Kumar, Vijay, Ong, Teng Aik, Mierzwa, Tiago Cesar, Adriansjah, Ricky, Banihani, Saleem A., Bowa, Kasonde, Fukuhara, Shinichiro, Peña, Marcelo Rodriguez, Moussa, Mohamad, Ari, Umut Çağın, Cho, Chak-Lam, Tadros, Nicholas N., Ugur, Muhammet Rasit, Amar, Edouard, Falcone, Marco, Santer, Frederic Romain, Kalkanli, Arif, Karna, Keshab Kumar, Khalafalla, Kareim, Vishwakarma, Ranjit B., Finocchi, Federica, Giulioni, Carlo, Ceyhan, Erman, Çeker, Gökhan, Yazbeck, Chadi, Rajmil, Osvaldo, Yilmaz, Mehmet, Altay, Baris, Barrett, Trenton L., Ngoo, Kay Seong, Roychoudhury, Shubhadeep, Salvio, Gianmaria, Lin, Haocheng, Kadioglu, Ates, Timpano, Massimiliano, Avidor-Reiss, Tomer, Hakim, Lukman, Sindhwani, Puneet, Franco, Giorgio, Singh, Rajender, Giacone, Filippo, Ruzaev, Mikhail, Kosgi, Raghavender, Sofikitis, Nikolaos, Palani, Ayad, Calik, Gokhan, Kulaksız, Deniz, Jezek, Davor, Al Hashmi, Manaf, Drakopoulos, Panagiotis, Omran, Huda, Leonardi, Sofia, Celik-Ozenci, Ciler, Güngör, Nur Dokuzeylül, Ramsay, Jonathan, Amano, Toshiyasu, Sogutdelen, Emrullah, Duarsa, Gede Wirya Kusuma, Chiba, Koji, Jindal, Sunil, Savira, Missy, Boeri, Luca, Borges, Edson, Gupte, Deepak, Gokalp, Fatih, Hebrard, Guadalupe Hernández, Minhas, Suks, Shah, Rupin, Farkouh, Ala’a, Agarwal, Ashok, Hamoda, Taha Abo-Almagd Abdel-Meguid, Kavoussi, Parviz, Saleh, Ramadan, Zini, Armand, Arafa, Mohamed, Harraz, Ahmed M., Gul, Murat, Karthikeyan, Vilvapathy Senguttuvan, Durairajanayagam, Damayanthi, Rambhatla, Amarnath, Boitrelle, Florence, Chung, Eric, Birowo, Ponco, Toprak, Tuncay, Ghayda, Ramy Abou, Cannarella, Rossella, Phuoc, Nguyen Ho Vinh, Dimitriadis, Fotios, Russo, Giorgio I., Sokolakis, Ioannis, Mostafa, Taymour, Makarounis, Konstantinos, Ziouziou, Imad, Kuroda, Shinnosuke, Bendayan, Marion, Kaiyal, Raneen Sawaid, Japari, Andrian, Simopoulou, Mara, Rocco, Lucia, Garrido, Nicolas, Gherabi, Nazim, Bocu, Kadir, Kahraman, Oguzhan, Le, Tan V., Wyns, Christine, Tremellen, Kelton, Sarikaya, Selcuk, Lewis, Sheena, Evenson, Donald P., Ko, Edmund, Calogero, Aldo E., Bahar, Fahmi, Martinez, Marlon, Crafa, Andrea, Nguyen, Quang, Ambar, Rafael F., Colpi, Giovanni, Bakircioglu, Mustafa Emre, Henkel, Ralf, Kandil, Hussein, Serefoglu, Ege Can, Alarbid, Abdullah, Tsujimura, Akira, Kheradmand, Alireza, Anagnostopoulou, Christina, Marino, Angelo, Adamyan, Aram, Zilaitiene, Birute, Ozer, Cevahir, Pescatori, Edoardo, Vogiatzi, Paraskevi, Busetto, Gian Maria, Balercia, Giancarlo, Elbardisi, Haitham, Akhavizadegan, Hamed, Sajadi, Hesamoddin, Taniguchi, Hisanori, Park, Hyun Jun, Rosas, Israel Maldonado, Al-Marhoon, Mohamed, Gilani, Mohammad Ali Sadighi, Alhathal, Naif, Pinggera, Germar-Michael, Kothari, Priyank, Mogharabian, Nasser, Micic, Sava, Homa, Sheryl T., Darbandi, Sara, Long, Tran Quang Tien, Zohdy, Wael, Atmoko, Widi, Sabbaghian, Marjan, Ibrahim, Wael, Smith, Ryan P., Ho, Christopher Chee Kong, Rosette, Jean de la, El-Sakka, Ahmed I., Preto, Mirko, Zenoaga-Barbăroșie, Cătălina, Abumelha, Saad Mohammed, Baser, Aykut, Aydos, Kaan, Ramirez-Dominguez, Liliana, Kumar, Vijay, Ong, Teng Aik, Mierzwa, Tiago Cesar, Adriansjah, Ricky, Banihani, Saleem A., Bowa, Kasonde, Fukuhara, Shinichiro, Peña, Marcelo Rodriguez, Moussa, Mohamad, Ari, Umut Çağın, Cho, Chak-Lam, Tadros, Nicholas N., Ugur, Muhammet Rasit, Amar, Edouard, Falcone, Marco, Santer, Frederic Romain, Kalkanli, Arif, Karna, Keshab Kumar, Khalafalla, Kareim, Vishwakarma, Ranjit B., Finocchi, Federica, Giulioni, Carlo, Ceyhan, Erman, Çeker, Gökhan, Yazbeck, Chadi, Rajmil, Osvaldo, Yilmaz, Mehmet, Altay, Baris, Barrett, Trenton L., Ngoo, Kay Seong, Roychoudhury, Shubhadeep, Salvio, Gianmaria, Lin, Haocheng, Kadioglu, Ates, Timpano, Massimiliano, Avidor-Reiss, Tomer, Hakim, Lukman, Sindhwani, Puneet, Franco, Giorgio, Singh, Rajender, Giacone, Filippo, Ruzaev, Mikhail, Kosgi, Raghavender, Sofikitis, Nikolaos, Palani, Ayad, Calik, Gokhan, Kulaksız, Deniz, Jezek, Davor, Al Hashmi, Manaf, Drakopoulos, Panagiotis, Omran, Huda, Leonardi, Sofia, Celik-Ozenci, Ciler, Güngör, Nur Dokuzeylül, Ramsay, Jonathan, Amano, Toshiyasu, Sogutdelen, Emrullah, Duarsa, Gede Wirya Kusuma, Chiba, Koji, Jindal, Sunil, Savira, Missy, Boeri, Luca, Borges, Edson, Gupte, Deepak, Gokalp, Fatih, Hebrard, Guadalupe Hernández, Minhas, Suks, and Shah, Rupin
- Abstract
Purpose Sperm DNA fragmentation (SDF) has been associated with male infertility and poor outcomes of assisted reproductive technology (ART). The purpose of this study was to investigate global practices related to the management of elevated SDF in infertile men, summarize the relevant professional society recommendations, and provide expert recommendations for managing this condition. Materials and Methods An online global survey on clinical practices related to SDF was disseminated to reproductive clinicians, according to the CHERRIES checklist criteria. Management protocols for various conditions associated with SDF were captured and compared to the relevant recommendations in professional society guidelines and the appropriate available evidence. Expert recommendations and consensus on the management of infertile men with elevated SDF were then formulated and adapted using the Delphi method. Results A total of 436 experts from 55 different countries submitted responses. As an initial approach, 79.1% of reproductive experts recommend lifestyle modifications for infertile men with elevated SDF, and 76.9% prescribe empiric antioxidants. Regarding antioxidant duration, 39.3% recommend 4–6 months and 38.1% recommend 3 months. For men with unexplained or idiopathic infertility, and couples experiencing recurrent miscarriages associated with elevated SDF, most respondents refer to ART 6 months after failure of conservative and empiric medical management. Infertile men with clinical varicocele, normal conventional semen parameters, and elevated SDF are offered varicocele repair immediately after diagnosis by 31.4%, and after failure of antioxidants and conservative measures by 40.9%. Sperm selection techniques and testicular sperm extraction are also management options for couples undergoing ART. For most questions, heterogenous practices were demonstrated. Conclusions This paper presents the results of a large global survey on the management of infertile men with elevat
- Published
- 2023
19. Efficient treatment of chronic endometritis through a novel approach of intrauterine antibiotic infusion: a case series
- Author
-
Sfakianoudis, Konstantinos, Simopoulou, Mara, Nikas, Yorgos, Rapani, Anna, Nitsos, Nikolaos, Pierouli, Katerina, Pappas, Athanasios, Pantou, Agni, Markomichali, Christina, Koutsilieris, Michael, and Pantos, Konstantinos
- Published
- 2018
- Full Text
- View/download PDF
20. Commercially Available Molecular Approaches to Evaluate Endometrial Receptivity: A Systematic Review and Critical Analysis of the Literature
- Author
-
Maziotis, Evangelos, primary, Kalampokas, Theodoros, additional, Giannelou, Polina, additional, Grigoriadis, Sokratis, additional, Rapani, Anna, additional, Anifantakis, Marios, additional, Kotsifaki, Amalia, additional, Pantou, Agni, additional, Triantafyllidou, Olga, additional, Tzanakaki, Despoina, additional, Neofytou, Spyridoula, additional, Vogiatzi, Paraskevi, additional, Bakas, Panagiotis, additional, Simopoulou, Mara, additional, and Vlahos, Nikolaos, additional
- Published
- 2022
- Full Text
- View/download PDF
21. Consensus and Diversity in the Management of Varicocele for Male Infertility: Results of a Global Practice Survey and Comparison with Guidelines and Recommendations
- Author
-
Shah, Rupin, Agarwal, Ashok, Kavoussi, Parviz, Rambhatla, Amarnath, Saleh, Ramadan, Cannarella, Rossella, Harraz, Ahmed M., Boitrelle, Florence, Kuroda, Shinnosuke, Hamoda, Taha Abo-Almagd Abdel-Meguid, Zini, Armand, Ko, Edmund, Çalık, Gökhan, Toprak, Tuncay, Kandil, Hussein, Gül, Murat, Bakırcıoğlu, Mustafa Emre, Parekh, Neel, Russo, Giorgio Ivan, Tadros, Nicholas, Kadıoğlu, Ateş, Arafa, Mohamed, Chung, Eric, Rajmil, Osvaldo, Dimitriadis, Fotios, Malhotra, Vineet, Salvio, Gianmaria, Henkel, Ralf, Le, Tan V., Sogutdelen, Emrullah, Vij, Sarah, Alarbid, Abdullah, Güdeloğlu, Ahmet, Tsujimura, Akira, Calogero, Aldo E., El Meliegy, Amr, Crafa, Andrea, Kalkanlı, Arif, Baser, Aykut, Hazır, Berk, Giulioni, Carlo, Cho, Chak-Lam, Ho, Christopher C.K., Salzano, Ciro, Zylbersztejn, Daniel Suslik, Tien, Dung Mai Ba, Pescatori, Edoardo, Borges, Edson, Saïs-Hamza, Eminej, Huyghe, Eric, Ceyhan, Erman, Caroppo, Ettore, Castiglioni, Fabrizio, Bahar, Fahmi, Gökalp, Fatih, Lombardo, Francesco, Gadda, Franco, Duarsa, Gede Wirya Kusuma, Pinggera, Germar-Michael, Busetto, Gian Maria, Balercia, Giancarlo, Cito, Gianmartin, Blecher, Gideon, Franco, Giorgio, Liguori, Giovanni, Elbardisi, Haitham, Keskin, Hakan, Lin, Haocheng, Taniguchi, Hisanori, Park, Hyun Jun, Ziouziou, Imad, de la Rosette, Jean J. M. C. H., Hotaling, Jim, Ramsay, Jonathan, Molina, Juan Manuel Corral, Lo, Ka Lun, Böcü, Kadir, Khalafalla, Kareim, Bowa, Kasonde, Okada, Keisuke, Nagao, Koichi, Chiba, Koji, Hakim, Lukman, Makarounis, Konstantinos, Hehemann, Marah, Peña, Marcelo Rodriguez, Falcone, Marco, Bendayan, Marion, Martinez, Marlon, Timpano, Massimiliano, Altan, Mesut, Fode, Mikkel, Al-Marhoon, Mohamed S., Gilani, Mohammad Ali Sadighi, Soebadi, Mohammad Ayodhia, Gherabi, Nazim, Sofikitis, Nikolaos, Kahraman, Oğuzhan, Birowo, Ponco, Kothari, Priyank, Sindhwani, Puneet, Javed, Qaisar, Ambar, Rafael F., Kosgi, Raghavender, Ghayda, Ramy Abou, Adriansjah, Ricky, Condorelli, Rosita Angela, La Vignera, Sandro, Micic, Sava, Kim, Shannon Hee Kyung, Fukuhara, Shinichiro, Ahn, Sun Tae, Mostafa, Taymour, Ong, Teng Aik, Takeshima, Teppei, Amano, Toshiyasu, Barrett, Trenton, Arslan, Umut, Karthikeyan, Vilvapathy Senguttuvan, Atmoko, Widi, Yumura, Yasushi, Yuan, Yiming, Kato, Yuki, Jezek, Davor, Cheng, Bryan Kwun-Chung, Hatzichristodoulou, Georgios, Dy, Jun, Castañé, Eduard Ruiz, El-Sakka, Ahmed I., Nguyen, Quang, Sarıkaya, Selçuk, Boeri, Luca, Tan, Ronny, Moussa, Mohamad A., El-Assmy, Ahmed, Alali, Hamed, Alhathal, Naif, Osman, Yasser, Perovic, Dragoljub, Sajadi, Hesamoddin, Akhavizadegan, Hamed, Vučinić, Miroslav, Kattan, Said, Kattan, Mohamed S., Mogharabian, Nasser, Phuoc, Nguyen Ho Vinh, Ngoo, Kay Seong, Alkandari, Mohammad H., Alsuhaibani, Shaheed, Sokolakis, Ioannis, Babaei, Mehdi, King, Mak Siu, Diemer, Thorsten, Gava, Marcelo M., Henrique, Raphael, Spinola e Silva, Rodrigo, Paul, Gustavo Marquesine, Mierzwa, Tiago Cesar, Glina, Sidney, Siddiqi, Kashif, Wu, Han, Wurzacher, Jana, Farkouh, Ala'a, Son, Hwancheol, Minhas, Suks, Lee, Joe, Magsanoc, Nikko, Capogrosso, Paolo, Albano, German Jose, Lewis, Sheena E.M., Jayasena, Channa N., Alvarez, Juan G., Teo, Colin, Smith, Ryan P., Chua, Jo Ben M., Jensen, Christian Fuglesang S., Parekattil, Sijo, Finelli, Renata, Durairajanayagam, Damayanthi, Karna, Keshab Kumar, Ahmed, Abdelkareem, Evenson, Don, Umemoto, Yukihiro, Puigvert, Ana, Çeker, Gökhan, Colpi, Giovanni M., Rolitsky, Sarah, Bouzouita, Abderrazak, Shokeir, Ahmed, Aşçı, Ahmet, Bouker, Amin, Adamyan, Aram, Avoyan, Armen E., Palani, Ayad, Aghamajidi, Azin, Eze, Balantine, Noegroho, Bambang Sasongko, Purnomo, Basuki, Erkan, Bircan Kolbaşı, Zilaitiene, Birute, Kulaksız, Deniz, Kafetzis, Dimitrios, Lee, Dong Sup, Stember, Doron, Evgeni, Evangelini, Alhajeri, Faisal, Finocchi, Federica, Colombo, Fulvio, Tsangaris, George, Sallam, Hassan N., Acosta, Herik, Rosas, Israel Maldonado, Kirkman-Brown, Jackson, Shin, Jae Il, Sonksen, Jens, Dong, Jie, Marmar, Joel, Moreno-Sepulveda, Jose, Seo, Ju Tae, Aydos, Kaan, Kesari, Kavindra Kumar, Trost, Landon, Jenkins, Lawrence, Rocco, Lucia, Darbandi, Mahsa, Simopoulou, Mara, Alves, Marco, Sabbaghian, Marjan, Tavalaee, Marziyeh, Razi, Mazdak, Duran, Mesut Berkan, Nago, Mitsuru, Elkhouly, Mohamed, Khalili, Mohamed, Nasr-Esfahani, Mohammad Hossein, Kamath, Mohan S., Uğur, Muhammet Raşit, Park, Nam Cheol, Cruz, Natalio, Garrido, Nicolas, Sodeifi, Niloofar, Al Khalidi, Noora, Shoshany, Ohad, Satyagraha, Paksi, Drakopoulos, Panagiotos, Vogiatzi, Paraskevi, Dolati, Parisa, Das, Partha, Chiu, Peter Ka-Fung, Tsioulou, Petroula A., Patel, Premal, Singh, Rajender, Kaiyal, Raneen Sawaid, Santos, Ferreira, Dada, Rima, Brodjonegoro, Sakti, Banihani, Saleem Ali, Schon, Samantha, Darbandi, Sara, Güneş, Sezgin, Homa, Sheryl, Mutambirwa, Shingai, Roychoudhury, Shubhadeep, Diaz, Sofia Ines Leonardi, Gopalakrishnan, Sreelatha, Krawetz, Stephen, Jindal, Sunil, Avidor-Reiss, Tomer, Lin, Tsung Yen, Kumar, Vijay, Ibrahim, Wael, Kerkeni, Walid, Woo, Wongi, Morimoto, Yoshiharu, Cheng, Yu-Sheng, Shah, Rupin, Agarwal, Ashok, Kavoussi, Parviz, Rambhatla, Amarnath, Saleh, Ramadan, Cannarella, Rossella, Harraz, Ahmed M., Boitrelle, Florence, Kuroda, Shinnosuke, Hamoda, Taha Abo-Almagd Abdel-Meguid, Zini, Armand, Ko, Edmund, Calik, Gokhan, Toprak, Tuncay, Kandil, Hussein, Gül, Murat, Bakırcıoğlu, Mustafa Emre, Parekh, Neel, Russo, Giorgio Ivan, Tadros, Nichola, Kadioglu, Ate, Arafa, Mohamed, Chung, Eric, Rajmil, Osvaldo, Dimitriadis, Fotio, Malhotra, Vineet, Salvio, Gianmaria, Henkel, Ralf, Le, Tan V., Sogutdelen, Emrullah, Vij, Sarah, Alarbid, Abdullah, Gudeloglu, Ahmet, Tsujimura, Akira, Calogero, Aldo E., Meliegy, Amr El, Crafa, Andrea, Kalkanli, Arif, Baser, Aykut, Hazir, Berk, Giulioni, Carlo, Cho, Chak-Lam, Ho, Christopher C. K., Salzano, Ciro, Zylbersztejn, Daniel Suslik, Tien, Dung Mai Ba, Pescatori, Edoardo, Borges, Edson, Serefoglu, Ege Can, Sas-Hamza, Emine, Huyghe, Eric, Ceyhan, Erman, Caroppo, Ettore, Castiglioni, Fabrizio, Bahar, Fahmi, Gokalp, Fatih, Lombardo, Francesco, Gadda, Franco, Duarsa, Gede Wirya Kusuma, Pinggera, Germar-Michael, Busetto, Gian Maria, Balercia, Giancarlo, Cito, Gianmartin, Blecher, Gideon, Franco, Giorgio, Liguori, Giovanni, Elbardisi, Haitham, Keskin, Hakan, Lin, Haocheng, Taniguchi, Hisanori, Park, Hyun Jun, Ziouziou, Imad, Rosette, Jean de la, Hotaling, Jim, Ramsay, Jonathan, Molina, Juan Manuel Corral, Lo, Ka Lun, Bocu, Kadir, Khalafalla, Kareim, Bowa, Kasonde, Okada, Keisuke, Nagao, Koichi, Chiba, Koji, Hakim, Lukman, Makarounis, Konstantino, Hehemann, Marah, Peña, Marcelo Rodriguez, Falcone, Marco, Bendayan, Marion, Martinez, Marlon, Timpano, Massimiliano, Altan, Mesut, Fode, Mikkel, Al-Marhoon, Mohamed S., Gilani, Mohammad Ali Sadighi, Soebadi, Mohammad Ayodhia, Gherabi, Nazim, Sofikitis, Nikolao, Kahraman, Oğuzhan, Birowo, Ponco, Kothari, Priyank, Sindhwani, Puneet, Javed, Qaisar, Ambar, Rafael F., Kosgi, Raghavender, Ghayda, Ramy Abou, Adriansjah, Ricky, Condorelli, Rosita Angela, Vignera, Sandro La, Micic, Sava, Kim, Shannon Hee Kyung, Fukuhara, Shinichiro, Ahn, Sun Tae, Mostafa, Taymour, Ong, Teng Aik, Takeshima, Teppei, Amano, Toshiyasu, Barrett, Trenton, Arslan, Umut, Karthikeyan, Vilvapathy Senguttuvan, Atmoko, Widi, Yumura, Yasushi, Yuan, Yiming, Kato, Yuki, Jezek, Davor, Cheng, Bryan Kwun-Chung, Hatzichristodoulou, Georgio, Dy, Jun, Castañé, Eduard Ruiz, El-Sakka, Ahmed I., Nguyen, Quang, Sarikaya, Selcuk, Boeri, Luca, Tan, Ronny, Moussa, Mohamad A., El-Assmy, Ahmed, Alali, Hamed, Alhathal, Naif, Osman, Yasser, Perovic, Dragoljub, Sajadi, Hesamoddin, Akhavizadegan, Hamed, Vučinić, Miroslav, Kattan, Said, Kattan, Mohamed S., Mogharabian, Nasser, Phuoc, Nguyen Ho Vinh, Ngoo, Kay Seong, Alkandari, Mohammad H., Alsuhaibani, Shaheed, Sokolakis, Ioanni, Babaei, Mehdi, King, Mak Siu, Diemer, Thorsten, Gava, Marcelo M., Henrique, Raphael, Silva, Rodrigo Spinola e, Paul, Gustavo Marquesine, Mierzwa, Tiago Cesar, Glina, Sidney, Siddiqi, Kashif, Wu, Han, Wurzacher, Jana, Farkouh, Ala’A, Son, Hwancheol, Minhas, Suk, Lee, Joe, Magsanoc, Nikko, Capogrosso, Paolo, Albano, German Jose, Lewis, Sheena E. M., Jayasena, Channa N., Alvarez, Juan G., Teo, Colin, Smith, Ryan P., Chua, Jo Ben M., Jensen, Christian Fuglesang S., Parekattil, Sijo, Finelli, Renata, Durairajanayagam, Damayanthi, Karna, Keshab Kumar, Ahmed, Abdelkareem, Evenson, Don, Umemoto, Yukihiro, Puigvert, Ana, Çeker, Gökhan, Forum, Giovanni M Colpi, Shah, R., Agarwal, A., Kavoussi, P., Rambhatla, A., Saleh, R., Cannarella, R., Harraz, A. M., Boitrelle, F., Kuroda, S., Hamoda, T. A. -A. A. -M., Zini, A., Ko, E., Calik, G., Toprak, T., Kandil, H., Gul, M., Bakircioglu, M. E., Parekh, N., Russo, G. I., Tadros, N., Kadioglu, A., Arafa, M., Chung, E., Rajmil, O., Dimitriadis, F., Malhotra, V., Salvio, G., Henkel, R., Le, T. V., Sogutdelen, E., Vij, S., Alarbid, A., Gudeloglu, A., Tsujimura, A., Calogero, A. E., El Meliegy, A., Crafa, A., Kalkanli, A., Baser, A., Hazir, B., Giulioni, C., Cho, C. -L., Ho, C. C. K., Salzano, C., Zylbersztejn, D. S., Tien, D. M. B., Pescatori, E., Borges, E., Serefoglu, E. C., Sais-Hamza, E., Huyghe, E., Ceyhan, E., Caroppo, E., Castiglioni, F., Bahar, F., Gokalp, F., Lombardo, F., Gadda, F., Duarsa, G. W. K., Pinggera, G. -M., Busetto, G. M., Balercia, G., Cito, G., Blecher, G., Franco, G., Liguori, G., Elbardisi, H., Keskin, H., Lin, H., Taniguchi, H., Park, H. J., Ziouziou, I., de la Rosette, J., Hotaling, J., Ramsay, J., Molina, J. M. C., Lo, K. L., Bocu, K., Khalafalla, K., Bowa, K., Okada, K., Nagao, K., Chiba, K., Hakim, L., Makarounis, K., Hehemann, M., Pena, M. R., Falcone, M., Bendayan, M., Martinez, M., Timpano, M., Altan, M., Fode, M., Al-Marhoon, M. S., Gilani, M. A. S., Soebadi, M. A., Gherabi, N., Sofikitis, N., Kahraman, O., Birowo, P., Kothari, P., Sindhwani, P., Javed, Q., Ambar, R. F., Kosgi, R., Ghayda, R. A., Adriansjah, R., Condorelli, R. A., La Vignera, S., Micic, S., Kim, S. H. K., Fukuhara, S., Ahn, S. T., Mostafa, T., Ong, T. A., Takeshima, T., Amano, T., Barrett, T., Arslan, U., Karthikeyan, V. S., Atmoko, W., Yumura, Y., Yuan, Y., Kato, Y., Jezek, D., Cheng, B. K. -C., Hatzichristodoulou, G., Dy, J., Castane, E. R., El-Sakka, A. I., Nguyen, Q., Sarikaya, S., Boeri, L., Tan, R., Moussa, M. A., El-Assmy, A., Alali, H., Alhathal, N., Osman, Y., Perovic, D., Sajadi, H., Akhavizadegan, H., Vucinic, M., Kattan, S., Kattan, M. S., Mogharabian, N., Phuoc, N. H. V., Ngoo, K. S., Alkandari, M. H., Alsuhaibani, S., Sokolakis, I., Babaei, M., King, M. S., Diemer, T., Gava, M. M., Henrique, R., Spinola e Silva, R., Paul, G. M., Mierzwa, T. C., Glina, S., Siddiqi, K., Wu, H., Wurzacher, J., Farkouh, A., Son, H., Minhas, S., Lee, J., Magsanoc, N., Capogrosso, P., Albano, G. J., Lewis, S. E. M., Jayasena, C. N., Alvarez, J. G., Teo, C., Smith, R. P., Chua, J. B. M., Jensen, C. F. S., Parekattil, S., Finelli, R., Durairajanayagam, D., Karna, K. K., Ahmed, A., Evenson, D., Umemoto, Y., Puigvert, A., Ceker, G., Colpi, G. M., Rolitsky, S., Bouzouita, A., Shokeir, A., Asci, A., Bouker, A., Adamyan, A., Avoyan, A. E., Palani, A., Aghamajidi, A., Eze, B., Noegroho, B. S., Purnomo, B., Erkan, B. K., Zilaitiene, B., Kulaksiz, D., Kafetzis, D., Lee, D. S., Stember, D., Evgeni, E., Alhajeri, F., Finocchi, F., Colombo, F., Tsangaris, G., Sallam, H. N., Acosta, H., Rosas, I. M., Kirkman-Brown, J., Shin, J. I., Sonksen, J., Dong, J., Marmar, J., Moreno-Sepulveda, J., Seo, J. T., Aydos, K., Kesari, K. K., Trost, L., Jenkins, L., Rocco, L., Darbandi, M., Simopoulou, M., Alves, M., Sabbaghian, M., Tavalaee, M., Razi, M., Duran, M. B., Nago, M., Elkhouly, M., Khalili, M., Nasr-Esfahani, M. H., Kamath, M. S., Ugur, M. R., Park, N. C., Cruz, N., Garrido, N., Sodeifi, N., Al Khalidi, N., Shoshany, O., Satyagraha, P., Drakopoulos, P., Vogiatzi, P., Dolati, P., Das, P., Chiu, P. K. -F., Tsioulou, P. A., Patel, P., Singh, R., Kaiyal, R. S., Santos, F., Dada, R., Brodjonegoro, S., Banihani, S. A., Schon, S., Darbandi, S., Gunes, S., Homa, S., Mutambirwa, S., Roychoudhury, S., Diaz, S. I. L., Gopalakrishnan, S., Krawetz, S., Jindal, S., Avidor-Reiss, T., Lin, T. Y., Kumar, V., Ibrahim, W., Kerkeni, W., Woo, W., Morimoto, Y., Cheng, Y. -S., and Tıp Fakültesi
- Subjects
Aging ,Consensus ,Urology ,INGUINAL VARICOCELECTOMY ,Disease management ,Male infertility ,Survey ,Varicocele ,Consensu ,Global Andrology Forum ,Endocrinology & Metabolism ,NONOBSTRUCTIVE AZOOSPERMIA ,ASSISTED REPRODUCTIVE TECHNOLOGY ,Male Infertility ,Pharmacology (medical) ,Andrology ,IMPAIRED SEMEN QUALITY ,EUROPEAN ASSOCIATION ,Science & Technology ,UROLOGY GUIDELINES ,Health Policy ,Public Health, Environmental and Occupational Health ,Disease Management ,Urology & Nephrology ,SUBCLINICAL VARICOCELE ,Psychiatry and Mental health ,Health Care Sciences & Services ,MICROSURGICAL SUBINGUINAL VARICOCELECTOMY ,Reproductive Medicine ,SPERM MORPHOLOGY ,UNTREATED VARICOCELE ,Life Sciences & Biomedicine - Abstract
Purpose: Varicocele is a common problem among infertile men. Varicocele repair (VR) is frequently performed to improve semen parameters and the chances of pregnancy. However, there is a lack of consensus about the diagnosis, indications for VR and its outcomes. The aim of this study was to explore global practice patterns on the management of varicocele in the context of male infertility. Materials and Methods: Sixty practicing urologists/andrologists from 23 countries contributed 382 multiple-choice-questions pertaining to varicocele management. These were condensed into an online questionnaire that was forwarded to clinicians involved in male infertility management through direct invitation. The results were analyzed for disagreement and agreement in practice patterns and, compared with the latest guidelines of international professional societies (American Urological As- sociation [AUA], American Society for Reproductive Medicine [ASRM], and European Association of Urology [EAU]), and with evidence emerging from recent systematic reviews and meta-analyses. Additionally, an expert opinion on each topic was provided based on the consensus of 16 experts in the field. Results: The questionnaire was answered by 574 clinicians from 59 countries. The majority of respondents were urologists/ uro-andrologists. A wide diversity of opinion was seen in every aspect of varicocele diagnosis, indications for repair, choice of technique, management of sub-clinical varicocele and the role of VR in azoospermia. A significant proportion of the re- sponses were at odds with the recommendations of AUA, ASRM, and EAU. A large number of clinical situations were identi- fied where no guidelines are available. Conclusions: This study is the largest global survey performed to date on the clinical management of varicocele for male in- fertility. It demonstrates: 1) a wide disagreement in the approach to varicocele management, 2) large gaps in the clinical prac- tice guidelines from professional societies, and 3) the need for further studies on several aspects of varicocele management in infertile men.
- Published
- 2022
22. Reporting on the Value of Artificial Intelligence in Predicting the Optimal Embryo for Transfer: A Systematic Review including Data Synthesis
- Author
-
Sfakianoudis, Konstantinos, primary, Maziotis, Evangelos, additional, Grigoriadis, Sokratis, additional, Pantou, Agni, additional, Kokkini, Georgia, additional, Trypidi, Anna, additional, Giannelou, Polina, additional, Zikopoulos, Athanasios, additional, Angeli, Irene, additional, Vaxevanoglou, Terpsithea, additional, Pantos, Konstantinos, additional, and Simopoulou, Mara, additional
- Published
- 2022
- Full Text
- View/download PDF
23. The Role of Interleukins in Recurrent Implantation Failure: A Comprehensive Review of the Literature
- Author
-
Pantos, Konstantinos, primary, Grigoriadis, Sokratis, additional, Maziotis, Evangelos, additional, Pistola, Kalliopi, additional, Xystra, Paraskevi, additional, Pantou, Agni, additional, Kokkali, Georgia, additional, Pappas, Athanasios, additional, Lambropoulou, Maria, additional, Sfakianoudis, Konstantinos, additional, and Simopoulou, Mara, additional
- Published
- 2022
- Full Text
- View/download PDF
24. Assessing the practice of LuPOR for poor responders: a prospective study evaluating follicular fluid cfDNA levels during natural IVF cycles
- Author
-
Rapani Anna, Tsioulou Petroula, Evangelos Maziotis, Koutsilieris Michael, Mastorakos George, Nezos Andrianos, Pantos Konstantinos, Sfakianoudis Konstantinos, Giannelou Polina, Pantou Agni, Grigoriadis Sokratis, Simopoulou Mara, and Glava Argyro
- Subjects
Adult ,0301 basic medicine ,media_common.quotation_subject ,Oocyte Retrieval ,Fertilization in Vitro ,Luteal Phase ,Luteal phase ,Cryopreservation ,Andrology ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Ovarian Follicle ,Ovulation Induction ,Alu Elements ,Follicular phase ,Genetics ,medicine ,Humans ,Blastocyst ,Assisted Reproduction Technologies ,Survival rate ,Genetics (clinical) ,Menstrual cycle ,media_common ,030219 obstetrics & reproductive medicine ,business.industry ,Obstetrics and Gynecology ,General Medicine ,Oocyte ,Follicular fluid ,Follicular Fluid ,030104 developmental biology ,medicine.anatomical_structure ,Follicular Phase ,Reproductive Medicine ,Oocytes ,Female ,business ,Cell-Free Nucleic Acids ,Infertility, Female ,Developmental Biology - Abstract
PURPOSE: The aim of this study is to provide data on the practice of Luteal Phase Oocyte Retrieval (LuPOR). The authors assess cell-free DNA levels in follicular fluid (ff cfDNA) from poor responders undergoing natural cycles, and comparing it to respective data originating from follicular phase oocyte retrievals. METHODS: Forty-seven women were eligible for this prospective study. Participants were classified as poor responders based on Bologna criteria while being detected with a second follicular wave. Follicular fluid was collected and prepared for cfDNA extraction. Levels of cfDNA were quantified via Q-PCR employing the ALU115 and ALU247 primers. These primers are associated with apoptotic and necrotic events. Levels of ff cfDNA resulting from follicular phase oocyte retrieval (FoPOR) and LuPOR-performed in a single menstrual cycle were associated with the number and maturation status of yielded oocytes and the number and fertilization status of resulting zygotes. Survival rate following thawing of cryopreserved zygotes, along with the resulting number of cleavage stage and blastocyst stage embryos are provided. RESULTS: Mean levels of ALU115 were significantly lower during FoPOR when compared to LuPOR (0.79 ± 0.72 vs 1.46 ± 1.59 ng/μl, p = 0.02). Regarding the FoPOR group, a significant positive correlation of serum estradiol and ALU115 concentration (p = 0.04) was revealed. A significant negative correlation between serum estradiol and cfDNA integrity was observed both during FoPOR (p = 0.03) and LuPOR (p = 0.03). A significant lower number of retrieved (1.09 ± 0.28 vs 1.29 ± 0.58, p = 0.02) and MII oocytes (0.77 ± 0.55 vs 1.08 ± 0.61, p = 0.02) was observed when comparing the FoPOR to LuPOR groups respectively. The integrity of cfDNA was observed to be higher in FoPOR originating embryos that arrested either prior to cleavage (0.28 ± 0.13 vs 0.17 ± 0.10, p = 0.006) or prior to blastocyst formation (0.28 ± 0.12 vs 0.13 ± 0.06, p = 0.04). In the case of LuPOR originating embryos, cfDNA integrity was observed to be higher in embryos that arrested only prior to the blastocyst stage (0.27 ± 0.20 vs 0.11 ± 0.07, p = 0.008). Similarly, cfDNA integrity was observed to be lower in top quality blastocysts originating from FoPOR (0.07 ± 0.04 vs 0.17 ± 0.05, p = 0.03) and in top quality cleavage stage embryos (0.09 ± 0.06 vs 0.31 ± 0.22, p = 0.01) and blastocysts (0.06 ± 0.02 vs 0.14 ± 0.06, p = 0.02) originating from LuPOR. CONCLUSIONS: Our results indicate that ff originating from LuPOR presents with higher levels of cfDNA. The higher cfDNA levels are attributed to mainly apoptotic events, as the ALU247 levels and DNA integrity did not differ statistically significantly between FoPOR and LuPOR. The absolute mean level of ALU247 corresponding to necrotic events was higher in LuPOR. Regarding embryological data, cfDNA integrity was correlated with both number and quality of cleavage stage embryos in both FoPOR and LuPOR, along with blastocyst stage embryos in LuPOR. Necrotic events were associated with poorer blastocyst formation rate and blastocyst quality in LuPOR. As the comparison between FoPOR and LuPOR results to similar IVF laboratory data, the practice of LuPOR may stand as a promising approach for poor responders, while it merits further investigation.
- Published
- 2020
25. Sperm vitality and necrozoospermia: Diagnosis, management, and results of a global survey of clinical practice
- Author
-
Agarwal, Ashok, Sharma, Rakesh K., Gupta, Sajal, Boitrelle, Florence, Finelli, Renata, Parekh, Neel, Durairajanayagam, Damayanthi, Saleh, Ramadan, Arafa, Mohamed, Cho, Chak Lam, Farkouh, Ala’a, Rambhatla, Amarnath, Henkel, Ralf, Vogiatzi, Paraskevi, Tadros, Nicholas, Kavoussi, Parviz, Ko, Edmund, Leisegang, Kristian, Kandil, Hussein, Palani, Ayad, Salvio, Gianmaria, Mostafa, Taymour, Rajmil, Osvaldo, Banihani, Saleem Ali, Schon, Samantha, Le, Tan V., Birowo, Ponco, Çeker, Gökhan, Alvarez, Juan, Molina, Juan Manuel Corral, Ho, Christopher C.K., Calogero, Aldo E., Khalafalla, Kareim, Duran, Mesut Berkan, Kuroda, Shinnosuke, Colpi, Giovanni M., Zini, Armand, Anagnostopoulou, Christina, Pescatori, Edoardo, Chung, Eric, Caroppo, Ettore, Dimitriadis, Fotios, Pinggera, Germar-Michael, Busetto, Gian Maria, Balercia, Giancarlo, Elbardisi, Haitham, Taniguchi, Hisanori, Park, Hyun Jun, Rosas, Israel Maldonado, de la Rosette, Jean J. M. C. H., Ramsay, Jonathan, Bowa, Kasonde, Simopoulou, Mara, Rodriguez, Marcelo Gabriel, Sabbaghian, Marjan, Martinez, Marlon, Gilani, Mohamed Ali Sadighi, Al-Marhoon, Mohamed S., Kosgi, Raghavender, Cannarella, Rossella, Micic, Sava, Fukuhara, Shinichiro, Parekattil, Sijo, Jindal, Sunil, Abdel-Meguid, Taha Abo-Almagd, Morimoto, Yoshiharu, and Shah, Rupin
- Subjects
Nigrosin ,endocrine system ,urogenital system ,Asthenozoospermia ,Infertility ,Eosine Yellowish-(YS) ,Vitality ,Spermatozoa - Abstract
Sperm vitality testing is a basic semen examination that has been described in the World Health Organization (WHO) Laboratory Manual for the Examination and Processing of Human Semen from its primary edition, 40 years ago. Several methods can be used to test sperm vitality, such as the eosin-nigrosin (E-N) stain or the hypoosmotic swelling (HOS) test. In the 6th (2021) edition of the WHO Laboratory Manual, sperm vitality assessment is mainly recommended if the total motility is less than 40%. Hence, a motile spermatozoon is considered alive, however, in certain conditions an immotile spermatozoon can also be alive. Therefore, the differentiation between asthenozoospermia (pathological decrease in sperm motility) and necrozoospermia (pathological decrease in sperm vitality) is important in directing further investigation and management of infertile patients. The causes leading to necrozoospermia are diverse and can either be local or general, testicular or extra -testicular. The andrological management of necrozoospermia depends on its etiology. However, there is no standardized treatment available presently and practice varies among clinicians. In this study, we report the results of a global survey to understand current practices regarding the physician order of sperm vitality tests as well as the management practices for necrozoospermia. Laboratory and clinical scenarios are presented to guide the reader in the management of necrozoospermia with the overall objective of establishing a benchmark ranging from the diagnosis of necrozoospermia by sperm vitality testing to its clinical management. American Center for Reproductive Medicine
- Published
- 2021
26. Sperm Vitality and Necrozoospermia: Diagnosis, Management, and Results of a Global Survey of Clinical Practice
- Author
-
Agarwal, Ashok, primary, Sharma, Rakesh K., additional, Gupta, Sajal, additional, Boitrelle, Florence, additional, Finelli, Renata, additional, Parekh, Neel, additional, Durairajanayagam, Damayanthi, additional, Saleh, Ramadan, additional, Arafa, Mohamed, additional, Cho, Chak Lam, additional, Farkouh, Ala'a, additional, Rambhatla, Amarnath, additional, Henkel, Ralf, additional, Vogiatzi, Paraskevi, additional, Tadros, Nicholas, additional, Kavoussi, Parviz, additional, Ko, Edmund, additional, Leisegang, Kristian, additional, Kandil, Hussein, additional, Palani, Ayad, additional, Salvio, Gianmaria, additional, Mostafa, Taymour, additional, Rajmil, Osvaldo, additional, Banihani, Saleem Ali, additional, Schon, Samantha, additional, Le, Tan V., additional, Birowo, Ponco, additional, Çeker, Gökhan, additional, Alvarez, Juan, additional, Molina, Juan Manuel Corral, additional, Ho, Christopher C.K., additional, Calogero, Aldo E., additional, Khalafalla, Kareim, additional, Duran, Mesut Berkan, additional, Kuroda, Shinnosuke, additional, Colpi, Giovanni M., additional, Zini, Armand, additional, Anagnostopoulou, Christina, additional, Pescatori, Edoardo, additional, Chung, Eric, additional, Caroppo, Ettore, additional, Dimitriadis, Fotios, additional, Pinggera, Germar-Michael, additional, Busetto, Gian Maria, additional, Balercia, Giancarlo, additional, Elbardisi, Haitham, additional, Taniguchi, Hisanori, additional, Park, Hyun Jun, additional, Maldonado Rosas, Israel, additional, de la Rosette, Jean, additional, Ramsay, Jonathan, additional, Bowa, Kasonde, additional, Simopoulou, Mara, additional, Rodriguez, Marcelo Gabriel, additional, Sabbaghian, Marjan, additional, Martinez, Marlon, additional, Gilani, Mohamed Ali Sadighi, additional, Al-Marhoon, Mohamed S., additional, Kosgi, Raghavender, additional, Cannarella, Rossella, additional, Micic, Sava, additional, Fukuhara, Shinichiro, additional, Parekattil, Sijo, additional, Jindal, Sunil, additional, Abdel-Meguid, Taha Abo-Almagd, additional, Morimoto, Yoshiharu, additional, and Shah, Rupin, additional
- Published
- 2022
- Full Text
- View/download PDF
27. A Comprehensive Guide to Sperm Recovery in Infertile Men with Retrograde Ejaculation
- Author
-
Gupta, Sajal, primary, Sharma, Rakesh, additional, Agarwal, Ashok, additional, Parekh, Neel, additional, Finelli, Renata, additional, Shah, Rupin, additional, Kandil, Hussein, additional, Saleh, Ramadan, additional, Arafa, Mohamed, additional, Ko, Edmund, additional, Simopoulou, Mara, additional, Zini, Armand, additional, Rajmil, Osvaldo, additional, Kavoussi, Parviz, additional, Singh, Keerti, additional, Ambar, Rafael F., additional, Elbardisi, Haitham, additional, Sengupta, Pallav, additional, Martinez, Marlon, additional, Boitrelle, Florence, additional, Alves, Marco G., additional, Khalafalla, Kareim, additional, Roychoudhury, Shubhadeep, additional, Busetto, Gian Maria, additional, Gosalvez, Jaime, additional, Tadros, Nicholas, additional, Palani, Ayad, additional, Rodriguez, Marcelo Gabriel, additional, Anagnostopoulou, Christina, additional, Micic, Sava, additional, Rocco, Lucia, additional, Mostafa, Taymour, additional, Alvarez, Juan G., additional, Jindal, Sunil, additional, Sallam, Hassan, additional, Rosas, Israel Maldonado, additional, Lewis, Sheena E. M., additional, AlSaid, Sami, additional, Altan, Mesut, additional, Park, Hyun Jun, additional, Ramsay, Jonathan, additional, Parekattil, Sijo, additional, Sabbaghian, Marjan, additional, Tremellen, Kelton, additional, Vogiatzi, Paraskevi, additional, Gilani, Mohammad Ali Sadighi, additional, Evenson, Donald P., additional, and Colpi, Giovanni M., additional
- Published
- 2022
- Full Text
- View/download PDF
28. Sperm Morphology Assessment in the Era of Intracytoplasmic Sperm Injection: Reliable Results Require Focus on Standardization, Quality Control, and Training
- Author
-
Agarwal, Ashok, primary, Sharma, Rakesh, additional, Gupta, Sajal, additional, Finelli, Renata, additional, Parekh, Neel, additional, Panner Selvam, Manesh Kumar, additional, Henkel, Ralf, additional, Durairajanayagam, Damayanthi, additional, Pompeu, Camila, additional, Madani, Sarah, additional, Belo, Andrea, additional, Singh, Neha, additional, Covarrubias, Simryn, additional, Darbandi, Sara, additional, Sadeghi, Raha, additional, Darbandi, Mahsa, additional, Vogiatzi, Paraskevi, additional, Boitrelle, Florence, additional, Simopoulou, Mara, additional, Saleh, Ramadan, additional, Arafa, Mohamed, additional, Majzoub, Ahmad, additional, Kandil, Hussein, additional, Zini, Armand, additional, Ko, Edmund, additional, Alvarez, Juan G., additional, Martinez, Marlon, additional, Ramsay, Jonathan, additional, Jindal, Sunil, additional, Busetto, Gian Maria, additional, Sallam, Hassan, additional, Maldonado, Israel, additional, Anagnostopoulou, Christina, additional, Alves, Marco G., additional, Sengupta, Pallav, additional, Gilany, Kambiz, additional, Evenson, Donald P., additional, Lewis, Sheena E.M., additional, Gosalvez, Jaime, additional, Ambar, Rafael F., additional, and Shah, Rupin, additional
- Published
- 2022
- Full Text
- View/download PDF
29. Post-Vasectomy Semen Analysis: Optimizing Laboratory Procedures and Test Interpretation through a Clinical Audit and Global Survey of Practices
- Author
-
Agarwal, Ashok, primary, Gupta, Sajal, additional, Sharma, Rakesh K., additional, Finelli, Renata, additional, Kuroda, Shinnosuke, additional, Vij, Sarah C., additional, Boitrelle, Florence, additional, Kavoussi, Parviz, additional, Rambhatla, Amarnath, additional, Saleh, Ramadan, additional, Chung, Eric, additional, Mostafa, Taymour, additional, Zini, Armand, additional, Ko, Edmund, additional, Parekh, Neel, additional, Martinez, Marlon, additional, Arafa, Mohamed, additional, Tadros, Nicholas, additional, de la Rosette, Jean, additional, Le, Tan V., additional, Rajmil, Osvaldo, additional, Kandil, Hussein, additional, Blecher, Gideon, additional, Liguori, Giovanni, additional, Caroppo, Ettore, additional, Ho, Christopher C.K., additional, Altman, Andrew, additional, Bajic, Petar, additional, Goldfarb, David, additional, Gill, Bradley, additional, Zylbersztejn, Daniel Suslik, additional, Molina, Juan Manuel Corral, additional, Gava, Marcello M., additional, Cardoso, Joao Paulo Greco, additional, Kosgi, Raghavender, additional, Çeker, Gökhan, additional, Zilaitiene, Birute, additional, Pescatori, Edoardo, additional, Borges, Edson, additional, Duarsa, Gede Wirya Kusuma, additional, Pinggera, Germar-Michael, additional, Busetto, Gian Maria, additional, Balercia, Giancarlo, additional, Franco, Giorgio, additional, Çalik, Gökhan, additional, Sallam, Hassan N., additional, Park, Hyun Jun, additional, Ramsay, Jonathan, additional, Alvarez, Juan, additional, Khalafalla, Kareim, additional, Bowa, Kasonde, additional, Hakim, Lukman, additional, Simopoulou, Mara, additional, Rodriguez, Marcelo Gabriel, additional, Sabbaghian, Marjan, additional, Elbardisi, Haitham, additional, Timpano, Massimiliano, additional, Altan, Mesut, additional, Elkhouly, Mohamed, additional, Al-Marhoon, Mohamed S., additional, Sadighi Gilani, Mohammad Ali, additional, Soebadi, Mohammad Ayodhia, additional, Nasr-Esfahani, Mohammad Hossein, additional, Garrido, Nicolas, additional, Vogiatzi, Paraskevi, additional, Birowo, Ponco, additional, Patel, Premal, additional, Javed, Qaisar, additional, Ambar, Rafael F., additional, Adriansjah, Ricky, additional, AlSaid, Sami, additional, Micic, Sava, additional, Lewis, Sheena E., additional, Mutambirwa, Shingai, additional, Fukuhara, Shinichiro, additional, Parekattil, Sijo, additional, Ahn, Sun Tae, additional, Jindal, Sunil, additional, Takeshima, Teppei, additional, Puigvert, Ana, additional, Amano, Toshiyasu, additional, Barrett, Trenton, additional, Toprak, Tuncay, additional, Malhotra, Vineet, additional, Atmoko, Widi, additional, Yumura, Yasushi, additional, Morimoto, Yoshiharu, additional, Lima, Thiago Fernandes Negris, additional, Kunz, Yannic, additional, Kato, Yuki, additional, Umemoto, Yukihiro, additional, Colpi, Giovanni M., additional, Durairajanayagam, Damayanthi, additional, and Shah, Rupin, additional
- Published
- 2022
- Full Text
- View/download PDF
30. Sperm DNA Fragmentation: A Critical Assessment of Clinical Practice Guidelines
- Author
-
Agarwal, Ashok, primary, Farkouh, Ala'a, additional, Parekh, Neel, additional, Zini, Armand, additional, Arafa, Mohamed, additional, Kandil, Hussein, additional, Tadros, Nick, additional, Busetto, Gian Maria, additional, Ambar, Rafael, additional, Parekattil, Sijo, additional, Boitrelle, Florence, additional, Sallam, Hassan, additional, Jindal, Sunil, additional, Ko, Edmund, additional, Simopoulou, Mara, additional, Park, Hyun Jun, additional, Sadighi, Mohammad Ali, additional, Saleh, Ramadan, additional, Ramsay, Jonathan, additional, Martinez, Marlon, additional, Elbardisi, Haitham, additional, Alvarez, Juan, additional, Colpi, Giovanni, additional, Gosalvez, Jaime, additional, Evenson, Donald, additional, and Shah, Rupin, additional
- Published
- 2022
- Full Text
- View/download PDF
31. Relevance of Leukocytospermia and Semen Culture and Its True Place in Diagnosing and Treating Male Infertility
- Author
-
Sharma, Rakesh, primary, Gupta, Sajal, additional, Agarwal, Ashok, additional, Henkel, Ralf, additional, Finelli, Renata, additional, Parekh, Neel, additional, Saleh, Ramadan, additional, Arafa, Mohamed, additional, Ko, Edmund, additional, Zini, Armand, additional, Tadros, Nicholas, additional, Shah, Rupin, additional, Ambar, Rafael F., additional, Elbardisi, Haitham, additional, Sengupta, Pallav, additional, Martinez, Marlon, additional, Boitrelle, Florence, additional, Simopoulou, Mara, additional, Vogiatzi, Paraskevi, additional, Gosalvez, Jaime, additional, Kavoussi, Parviz, additional, Kandil, Hussein, additional, Palani, Ayad, additional, Peña, Marcelo Rodriguez, additional, Rajmil, Osvaldo, additional, Busetto, Gian Maria, additional, Anagnostopoulou, Christina, additional, Micic, Sava, additional, Alves, Marco G., additional, Rocco, Lucia, additional, Mostafa, Taymour, additional, Alvarez, Juan G., additional, Jindal, Sunil, additional, Sallam, Hassan N., additional, Rosas, Israel Maldonado, additional, Lewis, Sheena E.M., additional, AlSaid, Sami, additional, Altan, Mesut, additional, Park, Hyun Jun, additional, Ramsay, Jonathan, additional, Parekattil, Sijo, additional, Sabbaghian, Marjan, additional, Tremellen, Kelton, additional, Khalafalla, Kareim, additional, Durairajanayagam, Damayanthi, additional, and Colpi, Giovanni M., additional
- Published
- 2022
- Full Text
- View/download PDF
32. Insights into the Role of Telomeres in Human Embryological Parameters. Opinions Regarding IVF
- Author
-
Anifandis, George, primary, Samara, Maria, additional, Simopoulou, Mara, additional, Messini, Christina I., additional, Chatzimeletiou, Katerina, additional, Thodou, Eleni, additional, Daponte, Alexandros, additional, and Georgiou, Ioannis, additional
- Published
- 2021
- Full Text
- View/download PDF
33. The Role of Uterine Natural Killer Cells on Recurrent Miscarriage and Recurrent Implantation Failure: From Pathophysiology to Treatment
- Author
-
Sfakianoudis, Konstantinos, primary, Rapani, Anna, additional, Grigoriadis, Sokratis, additional, Pantou, Agni, additional, Maziotis, Evangelos, additional, Kokkini, Georgia, additional, Tsirligkani, Chrysanthi, additional, Bolaris, Stamatis, additional, Nikolettos, Konstantinos, additional, Chronopoulou, Margarita, additional, Pantos, Konstantinos, additional, and Simopoulou, Mara, additional
- Published
- 2021
- Full Text
- View/download PDF
34. The Current Evidence Regarding COVID-19 and Pregnancy: Where Are We Now and Where Should We Head to Next?
- Author
-
Kalampokas, Theodoros, primary, Rapani, Anna, additional, Papageorgiou, Maria, additional, Grigoriadis, Sokratis, additional, Maziotis, Evangelos, additional, Anifandis, George, additional, Triantafyllidou, Olga, additional, Tzanakaki, Despoina, additional, Neofytou, Spyridoula, additional, Bakas, Panagiotis, additional, Simopoulou, Mara, additional, and Vlahos, Nikolaos, additional
- Published
- 2021
- Full Text
- View/download PDF
35. Empty Zona Pellucida Only Case: A Critical Review of the Literature
- Author
-
Siristatidis, Charalampos, primary, Tzanakaki, Despoina, additional, Simopoulou, Mara, additional, Vaitsopoulou, Christina, additional, Tsioulou, Petroula, additional, Stavros, Sofoklis, additional, Papapanou, Michail, additional, Drakakis, Peter, additional, Bakas, Panagiotis, additional, and Vlahos, Nikolaos, additional
- Published
- 2021
- Full Text
- View/download PDF
36. Molecular Drivers of Developmental Arrest in the Human Preimplantation Embryo: A Systematic Review and Critical Analysis Leading to Mapping Future Research
- Author
-
Sfakianoudis, Konstantinos, primary, Maziotis, Evangelos, additional, Karantzali, Eleni, additional, Kokkini, Georgia, additional, Grigoriadis, Sokratis, additional, Pantou, Amelia, additional, Giannelou, Polina, additional, Petroutsou, Konstantina, additional, Markomichali, Christina, additional, Fakiridou, Maria, additional, Koutsilieris, Michael, additional, Asimakopoulos, Byron, additional, Pantos, Konstantinos, additional, and Simopoulou, Mara, additional
- Published
- 2021
- Full Text
- View/download PDF
37. Introducing intrauterine antibiotic infusion as a novel approach in effectively treating chronic endometritis and restoring reproductive dynamics: a randomized pilot study
- Author
-
Pantos, Konstantinos, primary, Simopoulou, Mara, additional, Maziotis, Evangelos, additional, Rapani, Anna, additional, Grigoriadis, Sokratis, additional, Tsioulou, Petroula, additional, Giannelou, Polina, additional, Nitsos, Nikolaos, additional, Tzonis, Panagiotis, additional, Koutsilieris, Michael, additional, and Sfakianoudis, Konstantinos, additional
- Published
- 2021
- Full Text
- View/download PDF
38. Investigating the Role of the microRNA-34/449 Family in Male Infertility: A Critical Analysis and Review of the Literature
- Author
-
Pantos, Konstantinos, primary, Grigoriadis, Sokratis, additional, Tomara, Penelope, additional, Louka, Ioanna, additional, Maziotis, Evangelos, additional, Pantou, Agni, additional, Nitsos, Nikolaos, additional, Vaxevanoglou, Terpsithea, additional, Kokkali, Georgia, additional, Agarwal, Ashok, additional, Sfakianoudis, Konstantinos, additional, and Simopoulou, Mara, additional
- Published
- 2021
- Full Text
- View/download PDF
39. SARS-CoV-2 vs. human gametes, embryos and cryopreservation
- Author
-
Anifandis, George, primary, Messini, Christina I., additional, Simopoulou, Mara, additional, Sveronis, George, additional, Garas, Antonios, additional, Daponte, Alexandros, additional, and Messinis, Ioannis E., additional
- Published
- 2021
- Full Text
- View/download PDF
40. Evaluating different strategies for poor ovarian response management: a retrospective cohort study and literature review
- Author
-
Pantou, Agni, primary, Giannelou, Polina, additional, Grigoriadis, Sokratis, additional, Maziotis, Evangelos, additional, Tzonis, Panagiotis, additional, Koutsouni, Anastasia, additional, Pappa, Charikleia, additional, Philippou, Anastassios, additional, Koutsilieris, Michael, additional, Pantos, Konstantinos, additional, Simopoulou, Mara, additional, and Sfakianoudis, Konstantinos, additional
- Published
- 2021
- Full Text
- View/download PDF
41. The International Fertility Education Initiative: research and action to improve fertility awareness
- Author
-
Harper, Joyce C. Hammarberg, Karin Simopoulou, Mara Koert, Emily Pedro, Juliana Massin, Nathalie Fincham, Anita and Balen, Adam Int Fertility Educ Initiative
- Published
- 2021
42. SARS-CoV-2 vs. human gametes, embryos and cryopreservation
- Author
-
Anifandis, George Messini, Christina I. Simopoulou, Mara and Sveronis, George Garas, Antonios Daponte, Alexandros and Messinis, Ioannis E.
- Abstract
The COVID-19 pandemic, caused by the SARS-CoV-2 virus, is an unprecedented global situation, and all countries have adopted their own measurements to mitigate the spread of the virus in the first as well as in the subsequent waves of infection. All measures, especially in the first wave of the pandemic, were in combination with recommendations provided by professional and scientific organizations. Similar measures were applied to specific procedures, such as the management of infertility, including in vitro fertilization-embryo transfer (IVF-ET) treatments. Although there is no clear scientific evidence yet that the SARS-CoV-2 may exert negative effects on IVF outcome, especially at the early stages, several clinical reports indicate that the virus may impact male fertility through specific receptors presented at the somatic cells of the testis and used by the virus in order to gain entry to the respective cells. Nevertheless, it is not unreasonable to suspect that the virus may affect sperm function as well as oocyte performance directly through specific receptors or indirectly through other signaling pathways. Despite the good practice of IVF laboratory techniques, culture media may also be contaminated during equilibration when airborne virus's particles can contaminate culture media from an already infected embryology area or staff. Furthermore, although there is no clinical evidence, liquid nitrogen could be a route of infection for gametes and embryos when it has been contaminated during production or transportation. Therefore, cryopreservation of gametes and embryos must be virus-free. This communication aims to provide some aspects of the possible impact of the virus on gametes and embryos and how it may affect the cryopreservation procedures.
- Published
- 2021
43. Abnormal fertilization in ICSI and its association with abnormal semen parameters: a retrospective observational study on 1855 cases
- Author
-
Pantos, Konstantinos Sfakianoudis, Konstantinos Maziotis, Evangelos Rapani, Anna Karantzali, Eleni and Gounari-Papaioannou, Artemis Vaxevanoglou, Terpsithea and Koutsilieris, Michael Simopoulou, Mara
- Abstract
Intracytoplasmic sperm injection (ICSI) efficiently addresses male factor infertility. However, the occurrence of abnormal fertilization, mainly characterized by abnormal pronuclei (PN) patterns, merits investigation. To investigate abnormal fertilization patterns following ICSI and identify their respective associations with abnormal parameters in semen analysis (SA), a retrospective observational study including 1855 cycles was performed. Male infertility diagnosis relied on the 2010 WHO criteria. The population was divided into groups based on their SA results. The presence of 2PNs and extrusion of the second polar body (PB) indicated normal fertilization. A Kruskal-Wallis test along with a Wilcoxon post hoc evaluation and Bonferroni correction was employed for comparison among the groups. For the pregnancy rate, logistic regression was employed. No correlation was established between the SA abnormalities and the 1PN or 3PN formation rates. The highest and lowest 0PN rates were reported for the oligoasthenoteratozoospermic and normal groups, respectively. The lowest cleavage formation rates were identified in the oligoasthenozoospermic and oligoasthenoteratozoospermic groups. The aforementioned groups along with the oligoteratozoospermic group similarly presented the lowest blastocyst formation rates. For the clinical pregnancy rate, no statistically significant difference was observed. In conclusion, the incidence of two or more abnormal SA parameters - with the common denominator being oligozoospermia - may jeopardize normal fertilization, cleavage, and blastocyst rates. Once the developmental milestone of achieving blastocyst stage status was achieved, only oligoasthenozoospermia and oligoasthenoteratozoospermia were associated with lower rates. Interestingly, following adjustment for the number of blastocysts, no statistically significant differences were observed.
- Published
- 2021
44. Empty Zona Pellucida Only Case: A Critical Review of the Literature
- Author
-
Siristatidis, Charalampos Tzanakaki, Despoina Simopoulou, Mara and Vaitsopoulou, Christina Tsioulou, Petroula Stavros, Sofoklis and Papapanou, Michail Drakakis, Peter Bakas, Panagiotis and Vlahos, Nikolaos
- Subjects
endocrine system ,embryonic structures - Abstract
The presence of empty zona pellucida (EZP) in oocytes following oocyte retrieval (OR) during an in vitro fertilization (IVF) cycle presents a major clinical and laboratory challenge in assisted reproduction. It has been attributed to several factors such as the ovarian stimulation protocol employed, the damaging of the follicles during oocyte retrieval (OR) mainly through the high aspiration pressure, during the denudation technique, and the degeneration of oolemma within the zona pellucida (ZP) through apoptosis. The role of ZP is pivotal from the early stages of follicular development up to the preimplantation embryo development and embryo hatching. Polymorphisms or alterations on the genes that encode ZP proteins may contribute to EZP. We present a critical review of the published literature hitherto on EZP and available options when encountered with the phenomenon of EZP. Concerning the former, we found that there is rare data on this phenomenon that merits documentation. The latter includes technical, genetic, and pathophysiological perspectives, along with specific treatment options. In conclusion, we identify the lack of a definitive management proposal for couples presenting with this phenomenon, we underline the need for an algorithm, and indicate the questions raised that point towards our goal for a strategy when addressing a previous finding of EZP.
- Published
- 2021
45. Introducing intrauterine antibiotic infusion as a novel approach in effectively treating chronic endometritis and restoring reproductive dynamics: a randomized pilot study
- Author
-
Pantos, Konstantinos Simopoulou, Mara Maziotis, Evangelos and Rapani, Anna Grigoriadis, Sokratis Tsioulou, Petroula and Giannelou, Polina Nitsos, Nikolaos Tzonis, Panagiotis and Koutsilieris, Michael Sfakianoudis, Konstantinos
- Abstract
The chronic nature of Chronic Endometritis (CE) along with the challenging management and infertility entailed, call for cutting-edge therapeutic approaches. This study introduces the novel treatment of intrauterine antibiotic infusion (IAI) combined with oral antibiotic administration (OAA), and it assesses respective performance against the gold standard treatment of OAA. Data sourced herein reports on treatment efficiency and fertility restoration for both patients aiming to conceive naturally or via In Vitro fertilization. Eighty CE patients, 40 presenting with recurrent implantation failure, and 40 with recurrent pregnancy loss, were enrolled in the IVF and the natural conception arm respectively. Treatment was subjected to randomization. Effectively treated patients proceeded with either a single IVF cycle or were invited to conceive naturally over a 6-month period. Combination of IAI and OAA provided a statistically significant enhanced effectiveness treatment rate (RR 1.40; 95%CI 1.07-1.82; p=0.01). No statistically significant difference was observed regarding the side-effects rate (RR 1.33; 95%CI 0.80-2.22; p=0.52). No statistically significant difference was observed for either arm regarding live-birth rate. Following an intention-to-treat analysis, employment of IAI corresponds to improved clinical pregnancy rate-albeit not reaching statistical significance. In conclusion, complimentary implementation of IAI could provide a statistically significant enhanced clinical treatment outcome.
- Published
- 2021
46. Investigating the Role of the microRNA-34/449 Family in Male Infertility: A Critical Analysis and Review of the Literature
- Author
-
Pantos, Konstantinos Grigoriadis, Sokratis Tomara, Penelope and Louka, Ioanna Maziotis, Evangelos Pantou, Agni Nitsos, Nikolaos Vaxevanoglou, Terpsithea Kokkali, Georgia Agarwal, Ashok Sfakianoudis, Konstantinos Simopoulou, Mara
- Abstract
There is a great body of evidence suggesting that in both humans and animal models the microRNA-34/449 (miR-34/449) family plays a crucial role for normal testicular functionality as well as for successful spermatogenesis, regulating spermatozoa maturation and functionality. This review and critical analysis aims to summarize the potential mechanisms via which miR-34/449 dysregulation could lead to male infertility. Existing data indicate that miR-34/449 family members regulate ciliogenesis in the efferent ductules epithelium. Upon miR-34/449 dysregulation, ciliogenesis in the efferent ductules is significantly impaired, leading to sperm aggregation and agglutination as well as to defective reabsorption of the seminiferous tubular fluids. These events in turn cause obstruction of the efferent ductules and thus accumulation of the tubular fluids resulting to high hydrostatic pressure into the testis. High hydrostatic pressure progressively leads to testicular dysfunction as well as to spermatogenic failure and finally to male infertility, which could range from severe oligoasthenozoospermia to azoospermia. In addition, miR-34/449 family members act as significant regulators of spermatogenesis with an essential role in controlling expression patterns of several spermatogenesis-related proteins. It is demonstrated that these microRNAs are meiotic specific microRNAs as their expression is relatively higher at the initiation of meiotic divisions during spermatogenesis. Moreover, data indicate that these molecules are essential for proper formation as well as for proper function of spermatozoa per se. MicroRNA-34/449 family seems to exert significant anti-oxidant and anti-apoptotic properties and thus contribute to testicular homeostatic regulation. Considering the clinical significance of these microRNAs, data indicate that the altered expression of the miR-34/449 family members is strongly associated with several aspects of male infertility. Most importantly, miR-34/449 levels in spermatozoa, in testicular tissues as well as in seminal plasma seem to be directly associated with severity of male infertility, indicating that these microRNAs could serve as potential sensitive biomarkers for an accurate individualized differential diagnosis, as well as for the assessment of the severity of male factor infertility. In conclusion, dysregulation of miR-34/449 family detrimentally affects male reproductive potential, impairing both testicular functionality as well as spermatogenesis. Future studies are needed to verify these conclusions.
- Published
- 2021
47. Insights into the Role of Telomeres in Human Embryological Parameters. Opinions Regarding IVF
- Author
-
Anifandis, George Samara, Maria Simopoulou, Mara Messini, Christina I. Chatzimeletiou, Katerina Thodou, Eleni Daponte, Alexandros Georgiou, Ioannis
- Abstract
Telomeres promote genome integrity by protecting chromosome ends from the activation of the DNA damage response and protecting chromosomes from the loss of coding sequences due to the end replication problem. Telomere length (TL) is progressively shortened as age progresses, thus resulting in cellular senescence. Therefore, TL is in strong adverse linear correlation with aging. Mounting evidence supports the notion that telomeres and male/female infertility are in a close relationship, posing the biology of telomeres as a hot topic in the era of human-assisted reproduction. Specifically, the length of sperm telomeres is gradually increasing as men get older, while the telomere length of the oocytes seems not to follow similar patterns with that of sperm. Nonetheless, the telomere length of the embryos during the cleavage stages seems to have a paternal origin, but the telomere length can be further extended by telomerase activity during the blastocyst stage. The latter has been proposed as a new molecular biomarker with strong predictive value regarding male infertility. As far as the role of telomeres in assisted reproduction, the data is limited but the length of telomeres in both gametes seems to be affected mainly by the cause of infertility rather than the assisted reproductive therapy (ART) procedure itself. The present review aims to shed more light into the role of telomeres in human embryological parameters, including gametes and embryos and also presents opinions regarding the association between telomeres and in vitro fertilization (IVF).
- Published
- 2021
48. The International Fertility Education Initiative:research and action to improve fertility awareness
- Author
-
Harper, Joyce C, Hammarberg, Karin, Simopoulou, Mara, Koert, Emily, Pedro, Juliana, Massin, Nathalie, Fincham, Anita, Balen, Adam, Harper, Joyce C, Hammarberg, Karin, Simopoulou, Mara, Koert, Emily, Pedro, Juliana, Massin, Nathalie, Fincham, Anita, and Balen, Adam
- Published
- 2021
49. Getting to Know Endometriosis-Related Infertility Better: A Review on How Endometriosis Affects Oocyte Quality and Embryo Development
- Author
-
Simopoulou, Mara, primary, Rapani, Anna, additional, Grigoriadis, Sokratis, additional, Pantou, Agni, additional, Tsioulou, Petroula, additional, Maziotis, Evangelos, additional, Tzanakaki, Despina, additional, Triantafyllidou, Olga, additional, Kalampokas, Theodoros, additional, Siristatidis, Charalampos, additional, Bakas, Panagiotis, additional, and Vlahos, Nikolaos, additional
- Published
- 2021
- Full Text
- View/download PDF
50. New insights in addressing endometrial dysfunction: the potential role of growth hormone
- Author
-
Grigoriadis, Sokratis Maziotis, Evangelos Vlahos, Nikolaos and Simopoulou, Mara
- Published
- 2020
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.