29 results on '"Simonis N"'
Search Results
2. PP2A regulatory subunit B\u03b1 controls endothelial contractility and vessel lumen integrity via regulation of HDAC7
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Martin M, Geudens I, Bruyr J, Potente M, Bleuart A, Lebrun M, Simonis N, Deroanne C, Twizere JC, Soubeyran P, Peixoto P, Mottet D, Janssens V, Hofmann WK, Claes F, Carmeliet P, Kettmann R, Gerhardt H, and Dequiedt F.
- Published
- 2013
3. Modularity of the transcriptional response of protein complexes in yeast
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Simonis, N., Gonze, D., Orsi, C., Helden, J., Wodak, S. J., Laboratoire de Bioinformatique des Génomes et des Réseaux (BiGRe), Université libre de Bruxelles (ULB), and Spinelli, Lionel
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[SDV] Life Sciences [q-bio] ,[SDV]Life Sciences [q-bio] ,[INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM] ,ComputingMilieux_MISCELLANEOUS ,[INFO.INFO-BI] Computer Science [cs]/Bioinformatics [q-bio.QM] - Abstract
International audience; no abstract
- Published
- 2006
4. Transcriptional regulation of protein complexes in yeast
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Simonis, N., Wodak, S., Cohen, G., Jacques van Helden, Université libre de Bruxelles (ULB), and Spinelli, Lionel
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Saccharomyces cerevisiae Proteins ,Transcription, Genetic ,Macromolecular Substances ,Research ,[SDV]Life Sciences [q-bio] ,Genes, Fungal ,Computational Biology ,Biologie moléculaire ,Saccharomyces cerevisiae ,Regulon ,Nucleosomes ,[SDV] Life Sciences [q-bio] ,Predictive Value of Tests ,Gene Expression Regulation, Fungal ,Genes, Regulator ,[INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM] ,ComputingMilieux_MISCELLANEOUS ,[INFO.INFO-BI] Computer Science [cs]/Bioinformatics [q-bio.QM] - Abstract
This study shows that only a small fraction of yeast protein complexes are coregulated at the transcriptional level., Background Multiprotein complexes play an essential role in many cellular processes. But our knowledge of the mechanism of their formation, regulation and lifetimes is very limited. We investigated transcriptional regulation of protein complexes in yeast using two approaches. First, known regulons, manually curated or identified by genome-wide screens, were mapped onto the components of multiprotein complexes. The complexes comprised manually curated ones and those characterized by high-throughput analyses. Second, putative regulatory sequence motifs were identified in the upstream regions of the genes involved in individual complexes and regulons were predicted on the basis of these motifs. Results Only a very small fraction of the analyzed complexes (5-6%) have subsets of their components mapping onto known regulons. Likewise, regulatory motifs are detected in only about 8-15% of the complexes, and in those, about half of the components are on average part of predicted regulons. In the manually curated complexes, the so-called 'permanent' assemblies have a larger fraction of their components belonging to putative regulons than 'transient' complexes. For the noisier set of complexes identified by high-throughput screens, valuable insights are obtained into the function and regulation of individual genes. Conclusions A small fraction of the known multiprotein complexes in yeast seems to have at least a subset of their components co-regulated on the transcriptional level. Preliminary analysis of the regulatory motifs for these components suggests that the corresponding genes are likely to be co-regulated either together or in smaller subgroups, indicating that transcriptionally regulated modules might exist within complexes.
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- 2004
5. Autosomal recessive cerebellar ataxia of adult onset due to STUB1 Mutations
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Depondt, C., primary, Donatello, S., additional, Simonis, N., additional, Rai, M., additional, van Heurck, R., additional, Abramowicz, M., additional, D'Hooghe, M., additional, and Pandolfo, M., additional
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- 2014
- Full Text
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6. The angiographic course of minor and medium-grade coronary lesions is not influenced by high levels of lipoprotein (a)
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Bunte, T., primary, Bayerle, J.U., additional, Simonis, N., additional, Hahmann, H., additional, and Schieffer, H., additional
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- 1995
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7. Host-pathogen interactome mapping for HTLV-1 and -2 retroviruses
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Simonis Nicolas, Rual Jean-François, Lemmens Irma, Boxus Mathieu, Hirozane-Kishikawa Tomoko, Gatot Jean-Stéphane, Dricot Amélie, Hao Tong, Vertommen Didier, Legros Sébastien, Daakour Sarah, Klitgord Niels, Martin Maud, Willaert Jean-François, Dequiedt Franck, Navratil Vincent, Cusick Michael E, Burny Arsène, Van Lint Carine, Hill David E, Tavernier Jan, Kettmann Richard, Vidal Marc, and Twizere Jean-Claude
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HTLV ,Interactome ,Retrovirus ,ORFeome ,Tax ,HBZ ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Abstract Background Human T-cell leukemia virus type 1 (HTLV-1) and type 2 both target T lymphocytes, yet induce radically different phenotypic outcomes. HTLV-1 is a causative agent of Adult T-cell leukemia (ATL), whereas HTLV-2, highly similar to HTLV-1, causes no known overt disease. HTLV gene products are engaged in a dynamic struggle of activating and antagonistic interactions with host cells. Investigations focused on one or a few genes have identified several human factors interacting with HTLV viral proteins. Most of the available interaction data concern the highly investigated HTLV-1 Tax protein. Identifying shared and distinct host-pathogen protein interaction profiles for these two viruses would enlighten how they exploit distinctive or common strategies to subvert cellular pathways toward disease progression. Results We employ a scalable methodology for the systematic mapping and comparison of pathogen-host protein interactions that includes stringent yeast two-hybrid screening and systematic retest, as well as two independent validations through an additional protein interaction detection method and a functional transactivation assay. The final data set contained 166 interactions between 10 viral proteins and 122 human proteins. Among the 166 interactions identified, 87 and 79 involved HTLV-1 and HTLV-2 -encoded proteins, respectively. Targets for HTLV-1 and HTLV-2 proteins implicate a diverse set of cellular processes including the ubiquitin-proteasome system, the apoptosis, different cancer pathways and the Notch signaling pathway. Conclusions This study constitutes a first pass, with homogeneous data, at comparative analysis of host targets for HTLV-1 and -2 retroviruses, complements currently existing data for formulation of systems biology models of retroviral induced diseases and presents new insights on biological pathways involved in retroviral infection.
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- 2012
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8. An unusual presentation of de novo RAC3 variation in prenatal diagnosis.
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Meunier C, Cassart M, Kostyla K, Simonis N, Monestier O, and Tessier A
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- Pregnancy, Female, Humans, Prenatal Diagnosis, Agenesis of Corpus Callosum, Mutation, Missense, rac GTP-Binding Proteins genetics, Polymicrogyria, Nervous System Malformations, Hydrocephalus
- Abstract
Pathogenic variants in RAC3 cause a neurodevelopmental disorder with brain malformations and craniofacial dysmorphism, called NEDBAF. This gene encodes a small GTPase, which plays a critical role in neurogenesis and neuronal migration. We report a 31 weeks of gestation fetus with triventricular dilatation, and temporal and perisylvian polymicrogyria, without cerebellar, brainstem, or callosal anomalies. Trio whole exome sequencing identified a RAC3 (NM_005052.3, GRCh38) probably pathogenic de novo variant c.276 T>A p.(Asn92Lys). Eighteen patients harboring 13 different and essentially de novo missense RAC3 variants were previously reported. All the patients presented with corpus callosum malformations. Gyration disorders, ventriculomegaly (VM), and brainstem and cerebellar malformations have frequently been described. The only previous prenatal case associated with RAC3 variant presented with complex brain malformations, mainly consisting of midline and posterior fossa anomalies. We report the second prenatal case of NEDBAF presenting an undescribed pattern of cerebral anomalies, including VM and polymicrogyria, without callosal, cerebellar, or brainstem malformations. All neuroimaging data were reviewed to clarify the spectrum of cerebral malformations., (© 2024. The Author(s).)
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- 2024
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9. MME mutation in dominant spinocerebellar ataxia with neuropathy (SCA43).
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Depondt C, Donatello S, Rai M, Wang FC, Manto M, Simonis N, and Pandolfo M
- Abstract
Objective: To identify the causative gene mutation in a 5-generation Belgian family with dominantly inherited spinocerebellar ataxia and polyneuropathy, in which known genetic etiologies had been excluded., Methods: We collected DNA samples of 28 family members, including 7 living affected individuals, whose clinical records were reviewed by a neurologist experienced in ataxia. We combined linkage data of 21 family members with whole exome sequencing in 2 affected individuals to identify shared heterozygous variants mapping to potentially linked regions. Variants were screened for rarity and for predicted damaging effect. A candidate mutation was confirmed by Sanger sequencing and tested for cosegregation with the disease., Results: Affected individuals presented with late-onset sensorimotor axonal polyneuropathy; all but one also had cerebellar ataxia. We identified a variant in the MME gene, p.C143Y, that was absent from control databases, cosegregated with the phenotype, and was predicted to have a strong damaging effect on the encoded protein by all algorithms we used., Conclusions: MME encodes neprilysin (NEP), a zinc-dependent metalloprotease expressed in most tissues, including the central and peripheral nervous systems. The mutated cysteine 143 forms a disulfide bridge, which is 100% conserved in NEP and in similar enzymes. The recent identification of recessive MME mutations in 10 unrelated individuals from Japan with axonal polyneuropathy further supports the causality of the mutation, despite the dominant mode of inheritance and the presence of cerebellar involvement in our study family. Functional studies are needed to identify the mechanisms underlying these differences.
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- 2016
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10. The transcription factor ERG recruits CCR4-NOT to control mRNA decay and mitotic progression.
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Rambout X, Detiffe C, Bruyr J, Mariavelle E, Cherkaoui M, Brohée S, Demoitié P, Lebrun M, Soin R, Lesage B, Guedri K, Beullens M, Bollen M, Farazi TA, Kettmann R, Struman I, Hill DE, Vidal M, Kruys V, Simonis N, Twizere JC, and Dequiedt F
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- Aurora Kinases genetics, Aurora Kinases metabolism, Cell Line, Tumor, Fibroblasts cytology, Fibroblasts metabolism, HEK293 Cells, HeLa Cells, Humans, Osteoblasts cytology, Osteoblasts metabolism, Proto-Oncogene Protein c-fli-1 genetics, Proto-Oncogene Protein c-fli-1 metabolism, RNA Stability, RNA, Messenger metabolism, RNA, Small Interfering genetics, RNA, Small Interfering metabolism, RNA-Binding Proteins metabolism, Repressor Proteins antagonists & inhibitors, Repressor Proteins metabolism, Signal Transduction, Transcriptional Regulator ERG antagonists & inhibitors, Transcriptional Regulator ERG genetics, Transcriptional Regulator ERG metabolism, Mitosis, RNA Processing, Post-Transcriptional, RNA, Messenger genetics, RNA-Binding Proteins genetics, Repressor Proteins genetics
- Abstract
Control of mRNA levels, a fundamental aspect in the regulation of gene expression, is achieved through a balance between mRNA synthesis and decay. E26-related gene (Erg) proteins are canonical transcription factors whose previously described functions are confined to the control of mRNA synthesis. Here, we report that ERG also regulates gene expression by affecting mRNA stability and identify the molecular mechanisms underlying this function in human cells. ERG is recruited to mRNAs via interaction with the RNA-binding protein RBPMS, and it promotes mRNA decay by binding CNOT2, a component of the CCR4-NOT deadenylation complex. Transcriptome-wide mRNA stability analysis revealed that ERG controls the degradation of a subset of mRNAs highly connected to Aurora signaling, whose decay during S phase is necessary for mitotic progression. Our data indicate that control of gene expression by mammalian transcription factors may follow a more complex scheme than previously anticipated, integrating mRNA synthesis and degradation.
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- 2016
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11. Systematic interactome mapping of acute lymphoblastic leukemia cancer gene products reveals EXT-1 tumor suppressor as a Notch1 and FBWX7 common interactor.
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Daakour S, Hajingabo LJ, Kerselidou D, Devresse A, Kettmann R, Simonis N, Dequiedt F, and Twizere JC
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- Animals, Gene Expression Regulation, Neoplastic, HEK293 Cells, HeLa Cells, Humans, Mutation, Precursor Cell Lymphoblastic Leukemia-Lymphoma genetics, Protein Interaction Mapping, Signal Transduction, Transcription, Genetic, Zebrafish, N-Acetylglucosaminyltransferases metabolism, Precursor Cell Lymphoblastic Leukemia-Lymphoma metabolism, Receptor, Notch1 metabolism
- Abstract
Background: Perturbed genotypes in cancer can now be identified by whole genome sequencing of large number of diverse tumor samples, and observed gene mutations can be used for prognosis and classification of cancer subtypes. Although mutations in a few causative genes are directly linked to key signaling pathways perturbation, a global understanding of how known cancer genes drive oncogenesis in human is difficult to assess., Methods: We collected available information about mutated genes in Acute Lymphoblastic Leukemia (ALL). Validated human protein interactions (PPI) were collected from IntAct, HPRD and BioGRID interactomics databases, or obtained using yeast two-hybrid screening assay., Results: We have mapped interconnections between 116 cancer census gene products associated with ALL. Combining protein-protein interactions data and cancer-specific gene mutations information, we observed that 63 ALL-gene products are interconnected and identified 37 human proteins interacting with at least 2 ALL-gene products. We highlighted exclusive and coexistence genetic alterations in key signaling pathways including the PI3K/AKT and the NOTCH pathways. We then used different cell lines and reporter assay systems to validate the involvement of EXT1 in the Notch pathway., Conclusion: We propose that novel ALL-gene candidates can be identified based on their functional association with well-known cancer genes. We identified EXT1, a gene not previously linked to ALL via mutations, as a common interactor of NOTCH1 and FBXW7 regulating the NOTCH pathway in an FBXW7-dependend manner.
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- 2016
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12. Predicting interactome network perturbations in human cancer: application to gene fusions in acute lymphoblastic leukemia.
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Hajingabo LJ, Daakour S, Martin M, Grausenburger R, Panzer-Grümayer R, Dequiedt F, Simonis N, and Twizere JC
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- Blotting, Western, Cell Line, Tumor, Core Binding Factor Alpha 2 Subunit genetics, Core Binding Factor Alpha 2 Subunit metabolism, Fusion Proteins, bcr-abl genetics, Fusion Proteins, bcr-abl metabolism, Gene Expression Profiling, Gene Expression Regulation, Leukemic, Gene Fusion, HEK293 Cells, HeLa Cells, Humans, Microscopy, Confocal, Models, Genetic, Neoplasms metabolism, Oncogene Proteins, Fusion genetics, Oncogene Proteins, Fusion metabolism, Precursor B-Cell Lymphoblastic Leukemia-Lymphoma genetics, Precursor B-Cell Lymphoblastic Leukemia-Lymphoma metabolism, Precursor Cell Lymphoblastic Leukemia-Lymphoma genetics, Precursor Cell Lymphoblastic Leukemia-Lymphoma metabolism, Precursor Cell Lymphoblastic Leukemia-Lymphoma pathology, Protein Binding, Algorithms, Computational Biology methods, Gene Regulatory Networks genetics, Neoplasms genetics, Protein Interaction Maps genetics
- Abstract
Genomic variations such as point mutations and gene fusions are directly or indirectly associated with human diseases. They are recognized as diagnostic, prognostic markers and therapeutic targets. However, predicting the functional effect of these genetic alterations beyond affected genes and their products is challenging because diseased phenotypes are likely dependent of complex molecular interaction networks. Using as models three different chromosomal translocations-ETV6-RUNX1 (TEL-AML1), BCR-ABL1, and TCF3-PBX1 (E2A-PBX1)-frequently found in precursor-B-cell acute lymphoblastic leukemia (preB-ALL), we develop an approach to extract perturbed molecular interactions from gene expression changes. We show that the MYC and JunD transcriptional circuits are specifically deregulated after ETV6-RUNX1 and TCF3-PBX1 gene fusions, respectively. We also identified the bulk mRNA NXF1-dependent machinery as a direct target for the TCF3-PBX1 fusion protein. Through a novel approach combining gene expression and interactome data analysis, we provide new insight into TCF3-PBX1 and ETV6-RUNX1 acute lymphoblastic leukemia., (© 2014 Hajingabo et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).)
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- 2014
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13. tRNA methyltransferase homolog gene TRMT10A mutation in young onset diabetes and primary microcephaly in humans.
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Igoillo-Esteve M, Genin A, Lambert N, Désir J, Pirson I, Abdulkarim B, Simonis N, Drielsma A, Marselli L, Marchetti P, Vanderhaeghen P, Eizirik DL, Wuyts W, Julier C, Chakera AJ, Ellard S, Hattersley AT, Abramowicz M, and Cnop M
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- Adult, Age of Onset, Animals, Apoptosis genetics, Diabetes Mellitus, Type 2 complications, Female, Genetic Linkage, Humans, Insulin-Secreting Cells metabolism, Insulin-Secreting Cells pathology, Intellectual Disability complications, Intellectual Disability pathology, Male, Microcephaly complications, Microcephaly pathology, Mutation, Pedigree, Rats, Saccharomyces cerevisiae Proteins genetics, tRNA Methyltransferases deficiency, Diabetes Mellitus, Type 2 genetics, Intellectual Disability genetics, Methyltransferases genetics, Microcephaly genetics, tRNA Methyltransferases genetics
- Abstract
We describe a new syndrome of young onset diabetes, short stature and microcephaly with intellectual disability in a large consanguineous family with three affected children. Linkage analysis and whole exome sequencing were used to identify the causal nonsense mutation, which changed an arginine codon into a stop at position 127 of the tRNA methyltransferase homolog gene TRMT10A (also called RG9MTD2). TRMT10A mRNA and protein were absent in lymphoblasts from the affected siblings. TRMT10A is ubiquitously expressed but enriched in brain and pancreatic islets, consistent with the tissues affected in this syndrome. In situ hybridization studies showed that TRMT10A is expressed in human embryonic and fetal brain. TRMT10A is the mammalian ortholog of S. cerevisiae TRM10, previously shown to catalyze the methylation of guanine 9 (m(1)G9) in several tRNAs. Consistent with this putative function, in silico topology prediction indicated that TRMT10A has predominant nuclear localization, which we experimentally confirmed by immunofluorescence and confocal microscopy. TRMT10A localizes to the nucleolus of β- and non-β-cells, where tRNA modifications occur. TRMT10A silencing induces rat and human β-cell apoptosis. Taken together, we propose that TRMT10A deficiency negatively affects β-cell mass and the pool of neurons in the developing brain. This is the first study describing the impact of TRMT10A deficiency in mammals, highlighting a role in the pathogenesis of microcephaly and early onset diabetes. In light of the recent report that the type 2 diabetes candidate gene CDKAL1 is a tRNA methylthiotransferase, the findings in this family suggest broader relevance of tRNA methyltransferases in the pathogenesis of type 2 diabetes., Competing Interests: The authors have declared that no competing interests exist.
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- 2013
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14. FGFR1 mutations cause Hartsfield syndrome, the unique association of holoprosencephaly and ectrodactyly.
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Simonis N, Migeotte I, Lambert N, Perazzolo C, de Silva DC, Dimitrov B, Heinrichs C, Janssens S, Kerr B, Mortier G, Van Vliet G, Lepage P, Casimir G, Abramowicz M, Smits G, and Vilain C
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- Base Sequence, Binding Sites, Cleft Lip enzymology, Cleft Palate enzymology, Exome, Female, Genomics, Hand Deformities, Congenital enzymology, Holoprosencephaly enzymology, Humans, Intellectual Disability enzymology, Limb Deformities, Congenital enzymology, Male, Models, Molecular, Molecular Sequence Data, Polymorphism, Single Nucleotide, Receptor, Fibroblast Growth Factor, Type 1 chemistry, Sequence Analysis, DNA, Cleft Lip genetics, Cleft Palate genetics, Fingers abnormalities, Hand Deformities, Congenital genetics, Holoprosencephaly genetics, INDEL Mutation genetics, Intellectual Disability genetics, Limb Deformities, Congenital genetics, Receptor, Fibroblast Growth Factor, Type 1 genetics
- Abstract
Background: Harstfield syndrome is the rare and unique association of holoprosencephaly (HPE) and ectrodactyly, with or without cleft lip and palate, and variable additional features. All the reported cases occurred sporadically. Although several causal genes of HPE and ectrodactyly have been identified, the genetic cause of Hartsfield syndrome remains unknown. We hypothesised that a single key developmental gene may underlie the co-occurrence of HPE and ectrodactyly., Methods: We used whole exome sequencing in four isolated cases including one case-parents trio, and direct Sanger sequencing of three additional cases, to investigate the causative variants in Hartsfield syndrome., Results: We identified a novel FGFR1 mutation in six out of seven patients. Affected residues are highly conserved and are located in the extracellular binding domain of the receptor (two homozygous mutations) or the intracellular tyrosine kinase domain (four heterozygous de novo variants). Strikingly, among the six novel mutations, three are located in close proximity to the ATP's phosphates or the coordinating magnesium, with one position required for kinase activity, and three are adjacent to known mutations involved in Kallmann syndrome plus other developmental anomalies., Conclusions: Dominant or recessive FGFR1 mutations are responsible for Hartsfield syndrome, consistent with the known roles of FGFR1 in vertebrate ontogeny and conditional Fgfr1-deficient mice. Our study shows that, in humans, lack of accurate FGFR1 activation can disrupt both brain and hand/foot midline development, and that FGFR1 loss-of-function mutations are responsible for a wider spectrum of clinical anomalies than previously thought, ranging in severity from seemingly isolated hypogonadotropic hypogonadism, through Kallmann syndrome with or without additional features, to Hartsfield syndrome at its most severe end.
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- 2013
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15. Two novel CCDC88C mutations confirm the role of DAPLE in autosomal recessive congenital hydrocephalus.
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Drielsma A, Jalas C, Simonis N, Désir J, Simanovsky N, Pirson I, Elpeleg O, Abramowicz M, and Edvardson S
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- Adolescent, Adult, Child, Child, Preschool, Exons genetics, Female, Homozygote, Humans, Infant, Intracellular Signaling Peptides and Proteins, Male, PDZ Domains, Pedigree, Protein Binding, Radiography, Sequence Analysis, DNA, Codon, Nonsense genetics, Frameshift Mutation, Genes, Recessive, Hydrocephalus diagnostic imaging, Hydrocephalus genetics, Hydrocephalus pathology, Microfilament Proteins genetics
- Abstract
Background: Human congenital non-syndromic hydrocephalus is a vastly heterogeneous condition. A subgroup of cases are not secondary to a specific cause (eg, a neural tube defect), and within this subgroup, autosomal recessive inheritance has been described. One homozygous mutation in the DAPLE (Dvl-associating protein with a high frequency of leucine residues) protein-encoding gene CCDC88C (coiled-coil domain containing 88C) has recently been reported in a single family. The role of this gene has not been validated in another family, and no other autosomal recessive gene has been reported., Methods: We used homozygosity mapping and whole exome sequencing in two families with primary, non-syndromic congenital hydrocephalus from two different ethnic backgrounds., Results: In each family, we identified a novel homozygous mutation of CCDC88C. One mutation produced a premature stop codon at position 312 of the protein, while the second mutation induced a frameshift in the last exon, producing a stop codon that truncated the extreme C-terminus of DAPLE, including the 2026-2028 Gly-Cys-Val motif known to bind the post synaptic density protein (PSD95), Drosophila disc large tumor suppressor (Dlg1), and zonula occludens-1 protein (zo-1) (PDZ) domain of Dishevelled., Conclusions: Our data validate CCDC88C as causing autosomal recessive, primary non-syndromic congenital hydrocephalus, suggesting this gene may be an important cause of congenital hydrocephalus, and underscore the important role of the C-terminal PDZ domain-binding motif in the DAPLE protein.
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- 2012
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16. Protein interactions of the transcription factor Hoxa1.
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Lambert B, Vandeputte J, Remacle S, Bergiers I, Simonis N, Twizere JC, Vidal M, and Rezsohazy R
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- Animals, COS Cells, Chlorocebus aethiops, Green Fluorescent Proteins metabolism, HEK293 Cells, Humans, Mice, Pre-B-Cell Leukemia Transcription Factor 1, Protein Binding, Recombinant Fusion Proteins metabolism, TNF Receptor-Associated Factor 1 metabolism, Two-Hybrid System Techniques, Homeodomain Proteins metabolism, Protein Interaction Maps, Transcription Factors metabolism
- Abstract
Background: Hox proteins are transcription factors involved in crucial processes during animal development. Their mode of action remains scantily documented. While other families of transcription factors, like Smad or Stat, are known cell signaling transducers, such a function has never been squarely addressed for Hox proteins., Results: To investigate the mode of action of mammalian Hoxa1, we characterized its interactome by a systematic yeast two-hybrid screening against ~12,200 ORF-derived polypeptides. Fifty nine interactors were identified of which 45 could be confirmed by affinity co-purification in animal cell lines. Many Hoxa1 interactors are proteins involved in cell-signaling transduction, cell adhesion and vesicular trafficking. Forty-one interactions were detectable in live cells by Bimolecular Fluorescence Complementation which revealed distinctive intracellular patterns for these interactions consistent with the selective recruitment of Hoxa1 by subgroups of partner proteins at vesicular, cytoplasmic or nuclear compartments., Conclusions: The characterization of the Hoxa1 interactome presented here suggests unexplored roles for Hox proteins in cell-to-cell communication and cell physiology.
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- 2012
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17. Proto-genes and de novo gene birth.
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Carvunis AR, Rolland T, Wapinski I, Calderwood MA, Yildirim MA, Simonis N, Charloteaux B, Hidalgo CA, Barbette J, Santhanam B, Brar GA, Weissman JS, Regev A, Thierry-Mieg N, Cusick ME, and Vidal M
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- Base Sequence, Conserved Sequence, Genetic Variation, Molecular Sequence Data, Open Reading Frames, Phylogeny, Protein Biosynthesis, Saccharomyces classification, Saccharomyces cerevisiae classification, Saccharomyces cerevisiae genetics, Sequence Alignment, Evolution, Molecular, Genes, Fungal genetics, Saccharomyces genetics
- Abstract
Novel protein-coding genes can arise either through re-organization of pre-existing genes or de novo. Processes involving re-organization of pre-existing genes, notably after gene duplication, have been extensively described. In contrast, de novo gene birth remains poorly understood, mainly because translation of sequences devoid of genes, or 'non-genic' sequences, is expected to produce insignificant polypeptides rather than proteins with specific biological functions. Here we formalize an evolutionary model according to which functional genes evolve de novo through transitory proto-genes generated by widespread translational activity in non-genic sequences. Testing this model at the genome scale in Saccharomyces cerevisiae, we detect translation of hundreds of short species-specific open reading frames (ORFs) located in non-genic sequences. These translation events seem to provide adaptive potential, as suggested by their differential regulation upon stress and by signatures of retention by natural selection. In line with our model, we establish that S. cerevisiae ORFs can be placed within an evolutionary continuum ranging from non-genic sequences to genes. We identify ~1,900 candidate proto-genes among S. cerevisiae ORFs and find that de novo gene birth from such a reservoir may be more prevalent than sporadic gene duplication. Our work illustrates that evolution exploits seemingly dispensable sequences to generate adaptive functional innovation.
- Published
- 2012
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18. Viral perturbations of host networks reflect disease etiology.
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Gulbahce N, Yan H, Dricot A, Padi M, Byrdsong D, Franchi R, Lee DS, Rozenblatt-Rosen O, Mar JC, Calderwood MA, Baldwin A, Zhao B, Santhanam B, Braun P, Simonis N, Huh KW, Hellner K, Grace M, Chen A, Rubio R, Marto JA, Christakis NA, Kieff E, Roth FP, Roecklein-Canfield J, Decaprio JA, Cusick ME, Quackenbush J, Hill DE, Münger K, Vidal M, and Barabási AL
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- Computational Biology, Disease genetics, Fanconi Anemia etiology, Fanconi Anemia genetics, Fanconi Anemia virology, Genetic Predisposition to Disease, Herpesvirus 4, Human metabolism, Herpesvirus 4, Human pathogenicity, Host-Pathogen Interactions genetics, Host-Pathogen Interactions physiology, Human papillomavirus 16 metabolism, Human papillomavirus 16 pathogenicity, Humans, Protein Interaction Maps, Viral Proteins metabolism, Disease etiology, Models, Biological, Virus Diseases complications
- Abstract
Many human diseases, arising from mutations of disease susceptibility genes (genetic diseases), are also associated with viral infections (virally implicated diseases), either in a directly causal manner or by indirect associations. Here we examine whether viral perturbations of host interactome may underlie such virally implicated disease relationships. Using as models two different human viruses, Epstein-Barr virus (EBV) and human papillomavirus (HPV), we find that host targets of viral proteins reside in network proximity to products of disease susceptibility genes. Expression changes in virally implicated disease tissues and comorbidity patterns cluster significantly in the network vicinity of viral targets. The topological proximity found between cellular targets of viral proteins and disease genes was exploited to uncover a novel pathway linking HPV to Fanconi anemia.
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- 2012
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19. An empirical framework for binary interactome mapping.
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Venkatesan K, Rual JF, Vazquez A, Stelzl U, Lemmens I, Hirozane-Kishikawa T, Hao T, Zenkner M, Xin X, Goh KI, Yildirim MA, Simonis N, Heinzmann K, Gebreab F, Sahalie JM, Cevik S, Simon C, de Smet AS, Dann E, Smolyar A, Vinayagam A, Yu H, Szeto D, Borick H, Dricot A, Klitgord N, Murray RR, Lin C, Lalowski M, Timm J, Rau K, Boone C, Braun P, Cusick ME, Roth FP, Hill DE, Tavernier J, Wanker EE, Barabási AL, and Vidal M
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- Databases, Protein, Humans, Protein Binding, Proteins genetics, Sensitivity and Specificity, Protein Interaction Mapping methods, Proteins analysis, Proteins metabolism
- Abstract
Several attempts have been made to systematically map protein-protein interaction, or 'interactome', networks. However, it remains difficult to assess the quality and coverage of existing data sets. Here we describe a framework that uses an empirically-based approach to rigorously dissect quality parameters of currently available human interactome maps. Our results indicate that high-throughput yeast two-hybrid (HT-Y2H) interactions for human proteins are more precise than literature-curated interactions supported by a single publication, suggesting that HT-Y2H is suitable to map a significant portion of the human interactome. We estimate that the human interactome contains approximately 130,000 binary interactions, most of which remain to be mapped. Similar to estimates of DNA sequence data quality and genome size early in the Human Genome Project, estimates of protein interaction data quality and interactome size are crucial to establish the magnitude of the task of comprehensive human interactome mapping and to elucidate a path toward this goal.
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- 2009
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20. Edgetic perturbation models of human inherited disorders.
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Zhong Q, Simonis N, Li QR, Charloteaux B, Heuze F, Klitgord N, Tam S, Yu H, Venkatesan K, Mou D, Swearingen V, Yildirim MA, Yan H, Dricot A, Szeto D, Lin C, Hao T, Fan C, Milstein S, Dupuy D, Brasseur R, Hill DE, Cusick ME, and Vidal M
- Subjects
- Alleles, Disease genetics, Humans, Mutation genetics, Genetic Diseases, Inborn genetics, Models, Genetic
- Abstract
Cellular functions are mediated through complex systems of macromolecules and metabolites linked through biochemical and physical interactions, represented in interactome models as 'nodes' and 'edges', respectively. Better understanding of genotype-to-phenotype relationships in human disease will require modeling of how disease-causing mutations affect systems or interactome properties. Here we investigate how perturbations of interactome networks may differ between complete loss of gene products ('node removal') and interaction-specific or edge-specific ('edgetic') alterations. Global computational analyses of approximately 50,000 known causative mutations in human Mendelian disorders revealed clear separations of mutations probably corresponding to those of node removal versus edgetic perturbations. Experimental characterization of mutant alleles in various disorders identified diverse edgetic interaction profiles of mutant proteins, which correlated with distinct structural properties of disease proteins and disease mechanisms. Edgetic perturbations seem to confer distinct functional consequences from node removal because a large fraction of cases in which a single gene is linked to multiple disorders can be modeled by distinguishing edgetic network perturbations. Edgetic network perturbation models might improve both the understanding of dissemination of disease alleles in human populations and the development of molecular therapeutic strategies.
- Published
- 2009
- Full Text
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21. Empirically controlled mapping of the Caenorhabditis elegans protein-protein interactome network.
- Author
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Simonis N, Rual JF, Carvunis AR, Tasan M, Lemmens I, Hirozane-Kishikawa T, Hao T, Sahalie JM, Venkatesan K, Gebreab F, Cevik S, Klitgord N, Fan C, Braun P, Li N, Ayivi-Guedehoussou N, Dann E, Bertin N, Szeto D, Dricot A, Yildirim MA, Lin C, de Smet AS, Kao HL, Simon C, Smolyar A, Ahn JS, Tewari M, Boxem M, Milstein S, Yu H, Dreze M, Vandenhaute J, Gunsalus KC, Cusick ME, Hill DE, Tavernier J, Roth FP, and Vidal M
- Subjects
- Animals, Caenorhabditis elegans genetics, Caenorhabditis elegans Proteins genetics, Cell Line, Humans, Protein Binding, Software, Caenorhabditis elegans metabolism, Caenorhabditis elegans Proteins analysis, Caenorhabditis elegans Proteins metabolism, Protein Interaction Mapping methods
- Abstract
To provide accurate biological hypotheses and elucidate global properties of cellular networks, systematic identification of protein-protein interactions must meet high quality standards.We present an expanded C. elegans protein-protein interaction network, or 'interactome' map, derived from testing a matrix of approximately 10,000 x approximately 10,000 proteins using a highly specific, high-throughput yeast two-hybrid system. Through a new empirical quality control framework, we show that the resulting data set (Worm Interactome 2007, or WI-2007) was similar in quality to low-throughput data curated from the literature. We filtered previous interaction data sets and integrated them with WI-2007 to generate a high-confidence consolidated map (Worm Interactome version 8, or WI8). This work allowed us to estimate the size of the worm interactome at approximately 116,000 interactions. Comparison with other types of functional genomic data shows the complementarity of distinct experimental approaches in predicting different functional relationships between genes or proteins
- Published
- 2009
- Full Text
- View/download PDF
22. Literature-curated protein interaction datasets.
- Author
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Cusick ME, Yu H, Smolyar A, Venkatesan K, Carvunis AR, Simonis N, Rual JF, Borick H, Braun P, Dreze M, Vandenhaute J, Galli M, Yazaki J, Hill DE, Ecker JR, Roth FP, and Vidal M
- Subjects
- Animals, Databases, Factual, Humans, Protein Binding, Proteins analysis, Proteins chemistry, Reproducibility of Results, Research Design, Databases, Protein, Proteins metabolism
- Abstract
High-quality datasets are needed to understand how global and local properties of protein-protein interaction, or 'interactome', networks relate to biological mechanisms, and to guide research on individual proteins. In an evaluation of existing curation of protein interaction experiments reported in the literature, we found that curation can be error-prone and possibly of lower quality than commonly assumed.
- Published
- 2009
- Full Text
- View/download PDF
23. High-quality binary protein interaction map of the yeast interactome network.
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Yu H, Braun P, Yildirim MA, Lemmens I, Venkatesan K, Sahalie J, Hirozane-Kishikawa T, Gebreab F, Li N, Simonis N, Hao T, Rual JF, Dricot A, Vazquez A, Murray RR, Simon C, Tardivo L, Tam S, Svrzikapa N, Fan C, de Smet AS, Motyl A, Hudson ME, Park J, Xin X, Cusick ME, Moore T, Boone C, Snyder M, Roth FP, Barabási AL, Tavernier J, Hill DE, and Vidal M
- Subjects
- Computational Biology, Gene Regulatory Networks, Mass Spectrometry, Metabolic Networks and Pathways, Protein Array Analysis, Protein Binding, Proteome metabolism, Proteomics, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae Proteins genetics, Saccharomyces cerevisiae Proteins isolation & purification, Signal Transduction, Transcription Factors metabolism, Two-Hybrid System Techniques, Protein Interaction Mapping methods, Protein Interaction Mapping standards, Saccharomyces cerevisiae metabolism, Saccharomyces cerevisiae Proteins metabolism
- Abstract
Current yeast interactome network maps contain several hundred molecular complexes with limited and somewhat controversial representation of direct binary interactions. We carried out a comparative quality assessment of current yeast interactome data sets, demonstrating that high-throughput yeast two-hybrid (Y2H) screening provides high-quality binary interaction information. Because a large fraction of the yeast binary interactome remains to be mapped, we developed an empirically controlled mapping framework to produce a "second-generation" high-quality, high-throughput Y2H data set covering approximately 20% of all yeast binary interactions. Both Y2H and affinity purification followed by mass spectrometry (AP/MS) data are of equally high quality but of a fundamentally different and complementary nature, resulting in networks with different topological and biological properties. Compared to co-complex interactome models, this binary map is enriched for transient signaling interactions and intercomplex connections with a highly significant clustering between essential proteins. Rather than correlating with essentiality, protein connectivity correlates with genetic pleiotropy.
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- 2008
- Full Text
- View/download PDF
24. A protein domain-based interactome network for C. elegans early embryogenesis.
- Author
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Boxem M, Maliga Z, Klitgord N, Li N, Lemmens I, Mana M, de Lichtervelde L, Mul JD, van de Peut D, Devos M, Simonis N, Yildirim MA, Cokol M, Kao HL, de Smet AS, Wang H, Schlaitz AL, Hao T, Milstein S, Fan C, Tipsword M, Drew K, Galli M, Rhrissorrakrai K, Drechsel D, Koller D, Roth FP, Iakoucheva LM, Dunker AK, Bonneau R, Gunsalus KC, Hill DE, Piano F, Tavernier J, van den Heuvel S, Hyman AA, and Vidal M
- Subjects
- Animals, Cell Division, Protein Interaction Domains and Motifs, Proteome, Two-Hybrid System Techniques, Caenorhabditis elegans embryology, Embryo, Nonmammalian metabolism, Embryonic Development, Protein Interaction Mapping
- Abstract
Many protein-protein interactions are mediated through independently folding modular domains. Proteome-wide efforts to model protein-protein interaction or "interactome" networks have largely ignored this modular organization of proteins. We developed an experimental strategy to efficiently identify interaction domains and generated a domain-based interactome network for proteins involved in C. elegans early-embryonic cell divisions. Minimal interacting regions were identified for over 200 proteins, providing important information on their domain organization. Furthermore, our approach increased the sensitivity of the two-hybrid system, resulting in a more complete interactome network. This interactome modeling strategy revealed insights into C. elegans centrosome function and is applicable to other biological processes in this and other organisms.
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- 2008
- Full Text
- View/download PDF
25. Revisiting the Saccharomyces cerevisiae predicted ORFeome.
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Li QR, Carvunis AR, Yu H, Han JD, Zhong Q, Simonis N, Tam S, Hao T, Klitgord NJ, Dupuy D, Mou D, Wapinski I, Regev A, Hill DE, Cusick ME, and Vidal M
- Subjects
- Bayes Theorem, Conserved Sequence, Genomics, Proteomics, Open Reading Frames, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae Proteins genetics
- Abstract
Accurately defining the coding potential of an organism, i.e., all protein-encoding open reading frames (ORFs) or "ORFeome," is a prerequisite to fully understand its biology. ORFeome annotation involves iterative computational predictions from genome sequences combined with experimental verifications. Here we reexamine a set of Saccharomyces cerevisiae "orphan" ORFs recently removed from the original ORFeome annotation due to lack of conservation across evolutionarily related yeast species. We show that many orphan ORFs produce detectable transcripts and/or translated products in various functional genomics and proteomics experiments. By combining a naïve Bayes model that predicts the likelihood of an ORF to encode a functional product with experimental verification of strand-specific transcripts, we argue that orphan ORFs should still remain candidates for functional ORFs. In support of this model, interstrain intraspecies genome sequence variation is lower across orphan ORFs than in intergenic regions, indicating that orphan ORFs endure functional constraints and resist deleterious mutations. We conclude that ORFs should be evaluated based on multiple levels of evidence and not be removed from ORFeome annotation solely based on low sequence conservation in other species. Rather, such ORFs might be important for micro-evolutionary divergence between species.
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- 2008
- Full Text
- View/download PDF
26. Confirmation of organized modularity in the yeast interactome.
- Author
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Bertin N, Simonis N, Dupuy D, Cusick ME, Han JD, Fraser HB, Roth FP, and Vidal M
- Subjects
- Saccharomyces cerevisiae metabolism, Computational Biology methods, Databases, Genetic, Evolution, Molecular, Protein Interaction Mapping, Research Design, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae Proteins metabolism
- Abstract
Competing Interests: Competing interests. The authors have declared that no competing interests exist.
- Published
- 2007
- Full Text
- View/download PDF
27. Modularity of the transcriptional response of protein complexes in yeast.
- Author
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Simonis N, Gonze D, Orsi C, van Helden J, and Wodak SJ
- Subjects
- Cluster Analysis, DNA-Binding Proteins genetics, DNA-Binding Proteins metabolism, Databases, Protein, Down-Regulation, Gene Expression Profiling, Multiprotein Complexes, Nuclear Pore chemistry, Oligonucleotide Array Sequence Analysis, RNA Polymerase II genetics, RNA Polymerase II metabolism, Saccharomyces cerevisiae Proteins genetics, Up-Regulation, Gene Expression Regulation, Fungal, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae metabolism, Saccharomyces cerevisiae Proteins metabolism, Transcription, Genetic
- Abstract
A comprehensive study is performed on the condition-dependent expression of genes coding for the components of hand curated multi-protein complexes of the yeast Saccharomyces cerevisiae, in order to identify coherent transcriptional modules within these complexes. Such modules are defined as groups of genes within complexes whose expression profiles under a common set of experimental conditions allow us to discriminate them from random sets of genes. Our analysis reveals that complexes such as the cytoplasmic ribosome, the proteasome and the respiration chain complexes previously characterized as "stable" or "permanent" represent transcriptional modules that are coherently up or down-regulated in many different conditions. Overall however, some level of coherent expression is detected only in 71 out of the total of 113 complexes with at least five different protein components that could be reliably analyzed. Of these, 26 behave as coherently expressed transcriptional modules encompassing all the components of the complex. In another 15, at least half of the components make up such modules and in ten, few or no modules are detected. In an additional 20 complexes coherent expression is detected, but in too few conditions to enable reliable module detection. Interestingly, the transcriptional modules, when detected, often correspond to one or more known sub-complexes with specific functions. Furthermore, detected modules are generally consistent with transcriptional modules identified on the basis of predicted cis-regulatory sequence motifs. Also, groups of genes shared between complexes that carry out related functions tend to be part of overlapping transcriptional modules identified in these complexes. Together these findings suggest that transcriptional modules may represent basic functional and evolutionary building blocs of protein complexes.
- Published
- 2006
- Full Text
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28. Assessing computational tools for the discovery of transcription factor binding sites.
- Author
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Tompa M, Li N, Bailey TL, Church GM, De Moor B, Eskin E, Favorov AV, Frith MC, Fu Y, Kent WJ, Makeev VJ, Mironov AA, Noble WS, Pavesi G, Pesole G, Régnier M, Simonis N, Sinha S, Thijs G, van Helden J, Vandenbogaert M, Weng Z, Workman C, Ye C, and Zhu Z
- Subjects
- Amino Acid Motifs, Animals, Binding Sites, Databases, Protein, Drosophila, Fungal Proteins chemistry, Humans, Internet, Mice, Reproducibility of Results, Software, Computational Biology methods, Gene Expression, Transcription, Genetic
- Abstract
The prediction of regulatory elements is a problem where computational methods offer great hope. Over the past few years, numerous tools have become available for this task. The purpose of the current assessment is twofold: to provide some guidance to users regarding the accuracy of currently available tools in various settings, and to provide a benchmark of data sets for assessing future tools.
- Published
- 2005
- Full Text
- View/download PDF
29. Transcriptional regulation of protein complexes in yeast.
- Author
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Simonis N, van Helden J, Cohen GN, and Wodak SJ
- Subjects
- Computational Biology, Gene Expression Regulation, Fungal genetics, Genes, Fungal genetics, Genes, Regulator genetics, Macromolecular Substances, Nucleosomes chemistry, Nucleosomes metabolism, Predictive Value of Tests, Regulon genetics, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae Proteins genetics, Transcription, Genetic genetics
- Abstract
Background: Multiprotein complexes play an essential role in many cellular processes. But our knowledge of the mechanism of their formation, regulation and lifetimes is very limited. We investigated transcriptional regulation of protein complexes in yeast using two approaches. First, known regulons, manually curated or identified by genome-wide screens, were mapped onto the components of multiprotein complexes. The complexes comprised manually curated ones and those characterized by high-throughput analyses. Second, putative regulatory sequence motifs were identified in the upstream regions of the genes involved in individual complexes and regulons were predicted on the basis of these motifs., Results: Only a very small fraction of the analyzed complexes (5-6%) have subsets of their components mapping onto known regulons. Likewise, regulatory motifs are detected in only about 8-15% of the complexes, and in those, about half of the components are on average part of predicted regulons. In the manually curated complexes, the so-called 'permanent' assemblies have a larger fraction of their components belonging to putative regulons than 'transient' complexes. For the noisier set of complexes identified by high-throughput screens, valuable insights are obtained into the function and regulation of individual genes., Conclusions: A small fraction of the known multiprotein complexes in yeast seems to have at least a subset of their components co-regulated on the transcriptional level. Preliminary analysis of the regulatory motifs for these components suggests that the corresponding genes are likely to be co-regulated either together or in smaller subgroups, indicating that transcriptionally regulated modules might exist within complexes.
- Published
- 2004
- Full Text
- View/download PDF
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