1. Intrauterine Lipopolysaccharide-Induced Chorioamnionitis in a Sheep: Does It Affect the Auditory System?
- Author
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Simone M. Schaefer, Boris W. Kramer, Verena A.C. Lambermont, Adriana L. Smit, Luke W. G. Schoenmakers, Michelene N. Chenault, Robert J. Stokroos, Bernd Kremer, Lucien J. C. Anteunis, RS: CAPHRI School for Public Health and Primary Care, RS: MHeNs - R1 - Cognitive Neuropsychiatry and Clinical Neuroscience, MUMC+: MA Keel Neus Oorheelkunde (9), KNO, MUMC+: HZC Audiologisch Centrum Maastricht (9), Promovendi PHPC, FHML Methodologie & Statistiek, MUMC+: MA AIOS Keel Neus Oorheelkunde (9), RS: GROW - R2 - Basic and Translational Cancer Biology, RS: MHeNs - R3 - Neuroscience, RS: GROW - R4 - Reproductive and Perinatal Medicine, MUMC+: MA Medische Staf Kindergeneeskunde (9), Kindergeneeskunde, and RS: CAPHRI other
- Subjects
Lipopolysaccharides ,sheep ,medicine.medical_specialty ,Lipopolysaccharide ,medicine.medical_treatment ,Central nervous system ,Inflammation ,Chorioamnionitis ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Pregnancy ,Internal medicine ,Evoked Potentials, Auditory, Brain Stem ,Animals ,Medicine ,Saline ,hearing loss ,Fetus ,030219 obstetrics & reproductive medicine ,business.industry ,Interleukin-8 ,lipopolysaccharide ,Obstetrics and Gynecology ,auditory brain stem responses ,Perilymph ,medicine.disease ,chorioamnionitis ,Disease Models, Animal ,Endocrinology ,medicine.anatomical_structure ,chemistry ,In utero ,Immunology ,Female ,perilymph ,medicine.symptom ,business ,030217 neurology & neurosurgery - Abstract
Background: Fetal exposure to in utero inflammation such as chorioamnionitis is related to central nervous system injury. We hypothesized that chorioamnionitis can provoke inflammatory changes in the perilymph and alter hearing outcome. Methods: Pregnant ewes were randomized into 2 groups: intrauterine injection with lipopolysaccharide (LPS; n = 19) or saline (n = 21). In the first experiment, fetal perilymph samples were taken for cytokine analysis. In the second experiment, consecutive bone-conducted auditory brain stem responses were obtained from 1 to 7 months after birth. Results: Perilymph samples showed a significant elevation in interleukin 8 in the LPS group. Auditory brain stem response analysis demonstrated higher response thresholds and a prolongation of absolute peak V and interpeak intervals I to V and III to V in the LPS group compared to sham treatment. Conclusion: Our study confirms the hypothesis that an intrauterine inflammation by LPS can result in a fetal perilymphatic inflammatory response and functional impaired hearing outcomes after birth in a sheep model.
- Published
- 2016